基于地理探测器的流域土壤磷流失影响因素分析

基于修正的通用土壤流失方程(RUSLE)和地理探测器,定量分析了甘肃白龙江流域土壤磷流失的空间分异及其影响因素.结果表明:①2014年流域平均土壤磷流失量为0.214 t·km~(-2)·a~(-1),流失总量为3945.38 t·a~(-1);②磷流失空间分布较为破碎,热点区集中于迭部北部和舟曲东北部等高山区及舟曲、文县和武都三区县交界的低山河谷区,冷点区集聚于宕昌北部耕作区和文县南部森林保护区;③流域磷流失的空间分异主要受土地利用和地形的影响,农牧业生产也发挥了一定作用,磷流失整体表现为随海拔和坡度的抬升而增加,草地林地耕地;④流域及其内部冷热区影响因素差异较大,这是由各区地理环境协同差异所致,最大交互因子分别为土壤类型∩景观破碎度、土壤类型∩植被覆盖度、降水∩第一产业从业人口比重.在流域磷流失治理中,应合理安排农牧业生产,在提高植被覆盖度的同时降低景观破碎度.     

三江源植被保持土壤能力的时空变化

为深入了解三江源区植被保持土壤的能力,以RS和GIS为技术支撑,在对三江源区植被覆盖度动态变化特征分析的基础上,利用SL190—2007《土壤侵蚀分类分级标准》推荐的中国土壤侵蚀模型CSLE(Chinese soil loss equation)估算了2000—2010年三江源区植被的土壤保持能力,并分析其时空动态变化特征. 结果表明:①2000—2010年三江源区年均植被覆盖度为43%~50%;植被平均土壤保持量为849~955 t/km2,并且空间差异很大,总体上呈自西北向东南增加的空间分布格局. ②2000—2010年植被的土壤保持能力呈逐渐增加趋势,其中2000—2005年各流域的土壤保持能力平均增加了约29 t/km2,并以大夏河与洮河流域土壤保持能力的增幅(约700 t/km2)最大,其次为玛曲至龙羊峡(约300 t/km2),羌塘高原区植被的土壤保持能力增幅(仅2.08 t/km2)最小;2005—2010年各流域的土壤保持能力呈现轻微增长,平均增加了约9 t/km2,其中增幅较大的是柴达木盆地东和直门达至石鼓流域(约16 t/km2),增幅最小的是羌塘高原区(仅3.90 t/km2). ③三江源植被土壤保持能力随植被覆盖度的增加呈非线性增长,可通过指数或幂函数形式表达. 研究显示,增加植被覆盖度有助于提高三江源区的土壤保持能力、控制区域土壤侵蚀.      

门头沟生态系统土壤保持功能及其生态经济价值分析

在遥感和地理信息系统的支持下,利用通用土壤流失方程(USLE)和风力侵蚀模型,研究了门头沟生态系统土壤保持能力,并评价了其生态经济价值.结果表明:门头沟生态系统每年可以减少土壤的水蚀量为14.93×104 t/a,风蚀量为2 254.38×104 t/a;土壤保持能力在空间上呈现很大的差异性,总体上植被覆盖度高且坡度小的地区土壤保持能力最强,而植被覆盖度低且坡度较大的山地土壤保持能力最弱.土壤保持功能总价值为58 198.83×104　元/a,其中减少水蚀功能价值为442.83×104　元/a,减少风蚀功能价值为57 756×104　元/a.      

不同利用方式对草甸草原抗风蚀能力的影响

为了解不同利用方式对呼伦贝尔草甸草原抗风蚀能力的影响,采用植被凋查结合风洞实验的方法对不同利用方式条件下的草甸草原植被变化和草甸草原原状土的抗风蚀能力进行了研究.结果表明,草甸草原不同利用强度条件下临界风蚀风速(v)随植被盖度(Cover)的变化基本服从二次幂函数关系.随着利用强度的增加,植被盖度降低,在同样风速条件下(大于临界风蚀风速),风蚀率迅速增加.较轻的利用强度下,当植被盖度保持在63%时,在风速为25 m/s条件下风蚀率也很小,而当植被盖度<35%时,在风速为20～25 m/s条件下,风蚀率随植被盖度的下降迅速上升.草甸草原区的免耕农田由于完全失去植被的保护,每分钟狂风(25 m/s)造成的土壤风蚀量达到682.1 kg/hm2,接近1 a的土壤平均生成量(1 000 kg/hm2).     

水府庙水库流域土壤保持功能生态价值评估

在GIS和RS的支持下,基于改进的通用土壤流失方程(RUSLE)、影子价格法、机会成本法和替代工程法,以水府庙水库流域为研究对象,对该区域的土壤保持功能及价值的空间分布特征进行分析。研究表明,水府庙水库流域生态系统土壤保持总量为1.69×106t;生态总价值为91 829.72万元,其中生态系统土壤减少养分损失的价值为65 403.65万元、因防止土壤侵蚀而减少废弃地的价值为5 670.38万元、减轻泥沙淤积灾害方面的价值为20 755.69万元;土壤保持功能及其价值在空间上表现出很大的差异性,从流域中部地区向四周递增;土壤保持功能价值与植被覆盖度相关性较强,合理增加植被盖度对防治土壤侵蚀和增加土壤保持功能价值可以起到良好效果。     

焉耆盆地天然植被与地下水关系研究

根据不同水分、盐分梯度,将焉耆盆地分为河畔区、荒漠区和湖畔区。调查不同区天然植被种类、数量、盖度等因子与不同水分、盐分的关系,结果表明：河畔区从第一分水枢纽到开都河下游70km范围内,土壤水分无明显梯度变化,土壤盐分、地下水矿物质含量则呈明显增加趋势;随盐分梯度变化,乔、灌、草植被盖度与土壤含盐量呈指数关系;适宜植被生长的地下水埋深范围为0.5~2.4m,当地下水矿物质含量为0.62g/L时,河畔区植被生长最好。荒漠区（西区、南区、北区）从西北到东南方向,土壤水分、盐分均呈增加趋势;不同盐分条件对植被盖度有明显影响,当土壤含盐量在6~10g/kg之间时,随土壤含盐量增加,植被覆盖度降低,植被种类明显减少。湖畔区离湖1~3km范围,随离湖距离增加,土壤水分减小,盐分增大;随土壤含盐量增加,灌、草植被盖度逐渐减小,适宜植被生长的地下水埋深范围为0.5~5.7m;当地下水矿物质含量0.5g/L左右时,湖畔区植被生长最佳;地下水矿物质含量3~5g/L时,盐漠植被仍生长良好。     

安徽北部农村地区地下水重金属健康风险评价

为了解安徽北部农村地区地下水重金属污染对人类健康产生危害的风险,采集了26个农村地下水样品,测试分析了Mn、Zn、Cu、Pb、Ni、Cr和Cd七种重金属,并采用美国环境保护局(USEPA)推荐的风险评价模型对健康风险进行了初步评价。结果表明:非致癌污染物Mn、Zn、Cu、Pb和Ni的平均个人年风险分别为1.21×10~(-10)a~(-1)、8.46×10~(-11)a~(-1)、2.93×10~(-10)a~(-1)、8.29×10~(-10)a~(-1)和2.89×10~(-10)a~(-1),均远低于国际辐射防护委员会(ICRP)推荐的最大可接受值(5.0×10~(-5)a~(-1))和USEPA推荐的健康风险等级标准(1×10~(-4)a~(-1)),基本不会对暴露人群构成明显危害;致癌污染物Cd和Cr饮水途径健康危害平均个人年风险分别为1.44×10~(-6)a~(-1)和1.93×10~(-5)a~(-1),超出瑞典环境保护署、荷兰建设与环境部和英国皇家协会推荐标准(1×10~(-6)a~(-1)),Cr甚至超出19倍,为研究区首要污染物,应引起环境卫生部门的重视。     

孔雀河流域天然植被生态需水量估算

生态需水量的正确估算是实现流域水资源科学管理、合理配置的关键前提。本文综合多种方法计算了孔雀河流域天然植被不同保育目标和保护范围下的生态需水量。结果表明:若要保护流域内的所有天然植被,每年需要最低生态需水量为4.10×10~8m~3,其中林地需水0.4×10~8m~3,草地需水3.7×10~8m~3;若仅保护重点保护区内的天然植被,则每年需生态需水量为1.91×10~8m~3,其中林地需水量0.37×10~8m~3,草地需水量1.54×10~8m~3;若实施生态输水抢救工程,以保护"抢救"保护范围的天然植被,则每年需生态输水量为1.32×10~8m~3,其中良好林地需水0.18×10~8m~3,良好草地需水1.14×10~8m~3。     

黄土高原不同生态系统水土保持服务功能评价

以土壤保持量为评估指标,应用修正通用土壤流失方程,评估了黄土高原水土保持生态系统服务功能,分析了近20 a 来的黄土高原土壤保持量的空间分布及其动态变化,对于揭示全球气候变化背景下黄土高原林草植被建设的生态成效具有重要的科学价值和现实意义。结果表明：1990-2010 年黄土高原平均单位面积土壤保持量为305 t·hm^-2·a^-1,年均土壤保持总量为190×10⁸ t。1990-2000 年农田、草地和林地生态系统平均单位面积土壤保持量分别为249、285 和640 t·hm^-2·a^-1,2000-2010 年平均单位面积土壤保持量分别增加了14.6%、2.9%和7.4%。黄土高原草地和林地的土壤保持率分别为83%~88%和94%~97%。农田生态系统土壤保持量的空间分布特征表现为黄土丘陵沟壑区和黄土高塬沟壑区较大,农灌区和河谷平原区偏低;草地和林地生态系统土壤保持量的空间分布特征表现为沿东南向西北减少的变化趋势。与1990-2000 年不同,2000-2010 年农田、草地和林地生态系统土壤保持量的空间变化特征表现为较为明显的增长趋势,尤其是黄土丘陵沟壑区陕西榆林、延安地区和山西吕梁山区一带。     

五道梁高寒草原土壤水分和植被盖度空间异质性的地统计分析

青藏高原高寒草原区,由于长期冻融和地下冰的存在形成了独特的生态水文结构,土壤水分是控制高寒草原生态过程的关键因子。利用地统计学对多年冻土区高寒草原土壤表层含水量和植被盖度的空间变异性进行研究,结果表明,高寒草原生态系统浅层剖面(0~50 cm)土壤水分和植被盖度均符合正态分布,土壤含水量沿垂直方向逐渐增大,介于19.43%~25.37%之间,变异系数介于23.77%~40.92%;植被盖度具有强变异性,变异系数为47.99%。0~50 cm土壤含水量具有高度的空间异质性,其中91.1%的空间异质性是由空间自相关部分引起的,主要体现在10~190 m的中尺度上;植被盖度在研究尺度上具有中等程度的空间自相关,植被盖度随机部分的空间变异性占总空间变异性的比例为34.2%,主要体现在10 m的尺度内。各向异性分析表明,土壤水分和植被盖度具有明显的各向异性,其空间格局有明显的差异。     

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis （DGGE） and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity （both in gene richness and diversity index values） and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor （50 mg/kg） recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos （150 mg/kg） caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos （250 mg/kg） produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes （bands） responded differently to the impact of agrochemicals： four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the influence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affiliated with the alpha- and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.     

Arsenic uptake by arbuscular mycorrhizal maize （Zea mays L.） grown in an arsenic-contaminated soil with added phosphorus

The effects of arbuscular mycorrhizal (AM) fungus (Glomus mosseae) and phosphorus (P) addition (100 mg/kg soil) on arsenic (As) uptake by maize plants (Zea mays L.) from an As-contaminated soil were examined in a glasshouse experiment.Non-mycorrhizal and zero-P addition controls were included.Plant biomass and concentrations and uptake of As,P,and other nutrients,AM colonization,root lengths,and hyphal length densities were determined.The results indicated that addition of P significantly inhibited root colonization and development of extraradical mycelium.Root length and dry weight both increased markedly with mycorrhizal colonization under the zero-P treatments,but shoot and root biomass of AM plants was depressed by P application.AM fungal inoculation decreased shoot As concentrations when no P was added,and shoot and root As concentrations of AM plants increased 2.6 and 1.4 times with P addition,respectively.Shoot and root uptake of P,Mn,Cu,and Zn increased,but shoot Fe uptake decreased by 44.6%,with inoculation, when P was added.P addition reduced shoot P,Fe,Mn,Cu,and Zn uptake of AM plants,but increased root Fe and Mn uptake of the nonmycorrhizal ones.AM colonization therefore appeared to enhance plant tolerance to As in low P soil,and have some potential for the phytostabilization of As-contaminated soil,however,P application may introduce additional environmental risk by increasing soil As mobility.     

Impact of atrazine and nitrogen fertilizers on the sorption of chlorotoluron in soil and model sorbents

Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior of chlorotoluron. At the same concentration of N, sorption of chlorotoluron decreased as the concentration of atrazine increased on the day 0 and 6 in soil, respectively. The sorption of chlorotoluron increased from 0 to 6 d when soils were preincubated with deionized water, ammonium sulfate and urea solution for 6 d. That indicated incubation time was one of the most important factors for the sorption of chlorotoluron in nitrogen fertilizers treatments. The individual sorption isotherms of chlorotoluron in rubbery polymer and silica were strictly linear in single solute system, but there were competition sorption between pesticides or between pesticides and nitrogen fertilizers. That indicated the sorption taken place by concurrent solid-phase dissolution mechanism and sorption on the interface of water-organic matter or water-mineral matter.     

Degradation of metabolites accumulated of benzo[a]pyrene by coupling Penicillium Chrysogenum with KMnO4

Several main metabolites of benzo[a]pyrene （BaP） formed by Penicillium chrysogenum, Benzo[a]pyrene-1,6-quinone （BP 1,6- quinone）, trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene （BP 7,8-diol）, 3-hydroxybenzo[a]pyrene （3-OHBP）, were identified by high-performance liquid chromatography （HPLC）. The three metabolites were liable to be accumulated and were hardly further metabolized because of their toxicity to microorganisms. However, their further degradation was essential for the complete degradation of BaP. To enhance their degradation, two methods, degradation by coupling Penicillium chrysogenum with KMnO4 and degradation only by Penicillium chrysogenum, were compared; Meanwhile, the parameters of degradation in the superior method were optimized. The results showed that （1） the method of coupling Penicillium chrysogenum with KMnO4 was better and was the first method to be used in the degradation of BaP and its metabolites; （2） the metabolite, BP 1,6-quinone was the most liable to be accumulated in pure cultures; （3） the effect of degradation was the best when the concentration of KMnO4 in the cultures was 0.01% （w/v）, concentration of the three compounds was 5 mg/L and pH was 6.2. Based on the experimental results, a novel concept with regard to the bioremediation of BaP-contaminated environment was discussed, considering the influence on environmental toxicity of the accumulated metabolites.     

Potential of plant polyphenol oxidases in the decolorization and removal of textile and non-textile dyes

In this study an effort has been made to use plant polyphenol oxidases; potato （Solanum tuberosum） and brinjal （Solanum melongena）, for the treatment of various important dyes used in textile and other industries. The ammonium sulphate fractionated enzyme preparations were used to treat a number of dyes under various experimental conditions. Majority of the treated dyes were maximally decolorized at pH 3.0. Some of the dyes were quickly decolorized whereas others were marginally decolorized. The initial first hour was sufficient for the maximum decolorization of dyes. The rate of decolorization was quite slow on long treatment of dyes. Enhancement in the dye decolorization was noticed on increasing the concentration of enzymes. The complex mixtures of dyes were treated with both preparations of polyphenol oxidases in the buffers of varying pH values. Potato polyphenol oxidase was significantly more effective in decolorizing the dyes to higher extent as compared to the enzyme obtained from brinjal polyphenol oxidase. Decolorization of dyes and their mixtures, followed by the formation of an insoluble precipitate, which could be easily removed simply by centrifugation.     

Effects of two sludge application on fractionation and phytotoxicity of zinc and copper in the soil

The potential harm of heavy metals is a primary concern in application of sludge to the agricultural land. A pot experiment was conducted to evaluate the effect of two sludges on fractionation of Zn and Cu in soil and their phytotoxicity to pakchoi. The loamy soil was mixed with 0%, 20%, 40%, 60% and 80% (by weight) of digested sewage sludge (SS) and composted sludge (SC). The additions of both sludges caused a significant raise in all fractions, resulting in that exchangeable (EXCH) and organic bound (OM) became predominance of Zn and organic bound Cu occupied the largest portion. There was more available amount of Zn and Cu in SS treatments than SC treatments. During the pot experiment, the concentration of Zn in EXCH, carbonate (CAR) and OM and Cu in EXCH and OM fractions decreased in all treatments, so their bioavailability reduced. Germination rate and plant biomass decreased when the addition rate was high and the best yield appeared in 20% mixtures at the harvest of pakchoi. The two sludges increased tissue contents of Zn and Cu especially in the SS treatments. Zn in pakchoi was not only in relationship to ΔEXCH and ΔCAR forms but also in ΔOM forms in the sludge-soil mixtures. Tissue content of Cu in pakchoi grown on SC-soils could not be predicted by ΔEXCH. These correlation rates between Zn and Cu accumulation in pakchoi and variation of different fractions increased with time, which might indicate that sludges represented stronger impacts on the plant in long-term land application.     

Intraspecific differences in effects of co-contamination of cadmium and

A hydroponic experiment was carried out to study intraspecific differences in the effects of different concentrations of cadmium (Cd)(0-10 mg/L) and arsenate (As(V)) (0-8 mg/L) on the growth parameters and accumulation of Cd and As in six wheat varieties Jing-9428, Duokang-1, Jingdong-11, Jing-411, Jingdong-8 and Zhongmai-8. The endpoints of wheat seedlings, including seed germination,biomass, root length and shoot height, decreased with increasing the Cd and As concentrations. Significant differences in seed germination, biomass, root length, shoot height and the accumulation of Cd and As were observed between the treatments and among the varieties (p ＜ 0.05). The lethal dosage 50% were about 20, 80, 60, 60, 80 and 20 mg As/L for Jing-9428, Duokang-1, Jingdong-11,Jing-411, Jingdong-8 and Zhongmai-8, respectively, and the corresponding values for Cd were about 30, 80, 20, 40, 60 and 10 mg Cd/L, respectively. Among the six varieties, Duokang-1 was found to be the most resistant to Cd and As toxicity, and Zhongmai-8 was the most sensitive to Cd and As co-contamination. The resistance of the six varieties was found dependant on the seedling uptake of Cd and As. Duokang-1 was the most suitable for cultivation in Cd and As co-contaminated soils.     

Removal of heavy metals from sewage sludge by low costing chemical method and recycling in agriculture

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Isolation and preliminary characterization of a 3-chlorobenzoate degrading bacteria

A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several （halo）aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no （chloro）catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.     

Single and joint effects of pesticides and mercury on soil urease

The influence of two pesticides including chlorimuron-ethyl and furadan and mercury （Hg） on urease activity in 4 soils （meadow burozem and phaeozem） was investigated. The soils were exposed to various concentrations of the two pesticides and Hg individually and simultaneously. Results showed that there was a close relationship between urease activity and organic matter content in soil. Chlorimuron-ethyl and furadan could both activate urease in the 4 soils. The maximum increment of urease activity by chlorimuronethyl was up to 14%-18%. There was almost an equal increase （up to 13%-21%） in the urease activity by furadan. On the contrary, Hg markedly inhibited soil urease activity. A logarithmic equation was used to describe the relationship （P〈0.05） between the concentration of Hg and the activity of soil urease in the 4 tested soils. Semi-effect dose （ED50） values by the stress of Hg based on the inhibition of soil urease in the 4 soils were 88, 5.5, 24 and 20 mg/kg, respectively, according to the calculation of the corresponding equations. The interactive effect of chlorimuron-ethyl or furadan with metal Hg on soil urease was mainly synergic at the highest tested concentrations.