The use of the shanny Lipophrys pholis for pollution monitoring: a new sentinel species for the northwestern European marine ecosystems

The contamination of aquatic ecosystems by organic contaminants such as polycyclic aromatic hydrocarbons (PAHs) is a matter of great concern. Mussels have been extensively used as sentinel species in a large number of monitoring programs. However, the use of bivalves as the sole species has some limitations, because they are not as responsive as fish to Aryl Hydrocarbon Receptor agonists. Hence, for many marine areas, there is the need to validate new sentinel fish species that can be used in the assessment of pollution by organic contaminants. The shanny Lipophrys pholis is an intertidal fish that combines many characteristics required in a sentinel species: is abundant and easy to catch, has a wide geographical distribution and restrict home range. After larvae recruitment to the intertidal rocky shores, they remain in the same area for the rest of the life-cycle, thus reflecting local pollutants exposure. In order to evaluate the species sensitivity to organic contaminants under field conditions, L. pholis were collected at six sites reflecting different degrees of anthropogenic contamination. The induction of two biomarkers extensively validated in the assessment of PAHs contamination ethoxyresorufin-O-deethylase activity (EROD) and Fluorescent Aromatic Compounds (FACs) was evaluated. In parallel, mussels were collected at the same locations and levels of 16 PAHs and selected heavy metals determined. Overall, the specimens collected in the urban areas showed a significant induction of EROD and FACs (up to a six-fold induction) if compared with the reference sites. Additionally, a positive correlation was observed between the biomarkers and PAHs levels in mussel tissues. Even though further validation is currently in progress, the available data indicate that L. pholis is responsive to organic contaminants such as PAHs, suggesting its future integration in monitoring programmes designed to evaluate the presence of these contaminants in European marine ecosystems.     

New Aspect in Understanding the Biocenotic Role of Domestic Ungulates in the Life of Wolf (<Emphasis Type="Italic">Canis lupus</Emphasis> L.)

The dynamics of wolf attacks on domestic ungulates at the levels of population group (the region of Central Forest Nature Reserve) and population (Tver oblast) have been analyzed over the period of 1971 to 2015. It has been found that the frequency of attacks increases during the phase of predator population growth under the effect of qualitative changes within the population. An increase in the frequency of attacks and the number of sheep and calves killed per attack also takes place in the summer–autumn period, as a consequence of training wolf cubs in methods of killing prey. Therefore, domestic animals are not only a source of food for the wolf but also an object for training the young.     

Assessing field-scale migration of radionuclides at the Nevada Test Site: “mobile” species

Many long-lived radionuclides are present in groundwater at the Nevada Test Site (NTS) as a result of 828 underground nuclear weapons tests conducted between 1951 and 1992. In conjunction with a comprehensive geochemical review of radionuclides (³H, ¹⁴C, ³⁶Cl, ⁹⁹Tc and ¹²⁹I) that are presumably mobile in the subsurface, we synthesized a body of radionuclide activity data measured from groundwater samples collected at 18 monitoring wells, to qualitatively assess their migration at the NTS over distances of hundreds of meters and over timescales of decades. Tritium and ³⁶Cl showed little evidence of retardation, while the transport of ¹⁴C may have been retarded by its isotopic exchange with carbonate minerals in the aquifer. Observed local reducing conditions (either natural or test-induced) will impact the mobility of certain redox-sensitive radionuclides (especially ⁹⁹Tc) that were otherwise soluble and readily transported under oxidizing conditions. Conversely, strongly oxidizing conditions may impact the mobility of ¹²⁹I which is mobile under reducing conditions. The effect of iodine speciation on its transport deserves further attention. Indication of delayed transport of some “mobile” radionuclides (especially ⁹⁹Tc) in the groundwater at the NTS suggested the importance of redox conditions of the natural system in controlling the fate and transport of radionuclides, which has implications in the enhanced performance of the potential Yucca Mountain repository, located adjacent to the NTS, to store high-level nuclear wastes as well as management of radionuclide contamination in legacy nuclear operations facilities.     

Mortality and recruitment of mountain birch (<Emphasis Type="Italic">Betula pubescens</Emphasis> ssp. <Emphasis Type="Italic">czerepanovii</Emphasis>) in the impact zone of a copper-nickel smelter in the period of significant reduction of emissions: The results of 15-year monitoring

Long-term monitoring of mountain birch populations (1992–2006) was performed in 14 test plots located at distances of 1 to 63 km from the copper-nickel smelter in Monchegorsk (Murmansk oblast) and differing in the degree of disturbance. In the period from 1999 to 2006, atmospheric emissions of sulfur dioxide and heavy metals amounted to only one-third of those between 1992 and 1998, but birch mortality in heavily polluted areas (with nickel concentrations in leaves exceeding 160 mg/kg) remained at the same level, being absent (as previously) in less polluted areas. Throughout the observation period, birch recruitment was observed only in areas where nickel concentrations in the leaves were below 160 mg/kg; i.e., this concentration proved to be the threshold with respect to both mortality and recruitment of mountain birch. The course of demographic processes in its populations has remained unchanged after the reduction of emissions, confirming the hypothesis of the “inertial“ effect of industrial emissions on ecosystems. In some areas of industrial barrens, mountain birch may perish completely within the next decade.     

Assessment of the reproductive capacity of <Emphasis Type="Italic">Pinus sylvestris</Emphasis> trees growing under conditions of chronic exposure to radionuclides of uranium and thorium series

The reproductive capacity of Scots pine (Pinus sylvestris L.) has been estimated in trees from the population growing in an area used to store tailings from uranium ore processing and radium production. Under such conditions, 12- to 15-year-old trees produce seeds of a quality inferior to that in areas with a normal background radiation level. As the weighted absorbed dose rate (at a weighting coefficient of 5) for generative organs increases from 0.002 ± 0.0003 to 0.344 ± 0.059 mGy/day, a proportional increase is observed in the proportion of empty seeds and the frequency of cytogenetic disturbances in the root meristem of seedlings, while the survival rate of seedlings decreases. The minimum absorbed dose rate leading to these changes in the pine population is 0.027 mGy/day, i.e., 12 times as high as the control value.     

Genogeography of <Emphasis Type="Italic">Pinus sylvestris</Emphasis> L. populations in the Greater Caucasus and Crimea

Allozyme analysis of 18 Pinus sylvestris L. populations from seven landscape-geographic groups has been performed in the Greater Caucasus, Crimea, and Russian Plain. The results show that populations of the Mt. Elbrus region (isolated by high mountain ridges) are characterized by lower polymorphism and most distinct differentiation (at the level of geographic race) from other populations, which are differentiated from each other at the level of geographic groups. Genetic gradients (boundaries) between populations reach a maximum on transects across the Greater Caucasus Range, and Transcaucasian populations are more similar to populations of the Russian Plain, compared to North Caucasian populations, which confirms the hypothesis of ancestral connections between their gene pools.     

MANAGING POLLUTION FROM SSI<Emphasis Type="SmallCaps">s</Emphasis> – DESIGNING FOR A SUSTAINABLE INSTITUTION

The common effluent treatment plants (CETPs) are being propagated as the policy response to circumvent the pollution problem from the small-scale industries (SSIs), which abound in developing countries. The formation of the CETPs has been hailed as a collective action or co-operative solution to a common problem involving all the agents responsible for pollution. This paper investigated the desired criteria for designing an institution aimed at solving a common resource problem and factors ensuring the smooth functioning or sustainability of the institution. The design criteria and factors are then tested on a CETP set up in the Indian state of Haryana. The CETP, which was commissioned in 1997 had to be converted to a sewage treatment plant (STP) as the collective arrangement collapsed. The criteria used in the present analysis are that of Ostrom’s [Governing the Commons. Cambridge, UK, Cambridge University Press, 1990] design principles, whereas the factors considered for sustainability are the one suggested by Rawls [A Theory of Justice. USA, Harvard University Press, 1971]. The analysis indicates that the Kundli CETP failed in both counts as neither all the design principles were met nor did the institution fulfil different sustainability criteria. The paper concludes with the lessons that can be learnt from Kundli’s failure. Readers should send their comments on this paper to: BhaskarNath@aol.com within 3 months of publication of this issue.     

The role of interactions between individual animals in the response to heat stress in the freshwater crustacean <Emphasis Type="Italic">Daphnia magna</Emphasis>

The thermal resistance of Daphnia magna Straus is a trait depending on the interaction between individuals. The group effect is expressed in D. magna as both a decrease in thermal resistance and a marked individual differentiation with respect to thermal resistance. Two types of interactions between individual D. magna have been found: (1) the release of an unidentified factor decreasing thermal resistance (without noticeably changing the phenotypic variability of this trait) into the medium and (2) a decrease in the thermal resistance of individuals poorly adapted to heat stress, with the thermal resistance of well-adapted individuals (determined by the dependence of thermal resistance on interactions between individuals) remaining the same or even slightly increasing.Translated from Ekologiya, No. 2, 2005, pp. 126–131.Original Russian Text Copyright © 2005 by Kalinnikova, Krasnoperova, T. Gainutdinov, Sadykova, M. Gainutdinov.     

Faunal diversity and dynamics of species richness and dominance of parasite communities in age series of the perch (<Emphasis Type="Italic">Perca fluviatilis</Emphasis>)

Changes in parameters of species richness and dominance in parasite communities of perch have been analyzed in the age series of hosts from two water bodies, Lake Gusinoe and Chivyrkui Bay (Lake Baikal), at the levels of a host individual (infracommunity) and a host age group (set of infracommunities). The species richness of parasite infracommunities has been found to increase in both age series due to decreasing proportions of fish affected by no more than three to four parasite species and increasing proportion of fish parasitized by more than four to five species.     

Influence of silts on the growth,reproduction and chlorophyll fluorescence of <Emphasis Type="Italic">Potamogeton crispus</Emphasis> in turbid water

Turions of Potamogeton crispus were planted in several turbid waters. Some parameters of growth, reproduction and chlorophyll fluorescence were measured. The results were as follows: (1) Sprouting rates in all groups reached to 100.0% on the 17^th day. (2) The parameters of the growth (stem and leaf) and chlorophyll fluorescence (Φ_PSII, qP and qN) in 120 and 150NTU waters were inhibited heavily 60 days later, and their survival time was only 140 days. However, the plants in 30 to 90NTU waters could grow near water surface, their survival time was about 190 days. (3) After 150 days, the saturating irradiance intensity and rETR_max of the leaves in 30 to 90NTU waters markedly decreased with an increase in the amount of attached silts, and the number and fresh mass of turions (propagula) in 60 and 90NTU waters were significantly lower than the control. In summary, P. crispus in 0.7-metre-deep turbid water with suspended silts (not more than 90NTU) could sprout and grow normally, and produce the turions.     

Biosphere modelling for the assessment of radioactive waste repositories; the development of a common basis by the BIOMOVS II reference biospheres working group

Performance criteria for radioactive waste repositories are often expressed in terms of dose or risk. The characteristics of biosphere modelling for performance assessment are that: (a) potential release occurs in the distant future, (b) reliable predictions of human behaviour at the time of release are impracticable, and (c) the biosphere is not considered to be a barrier as the geosphere and the engineered barriers. For these and other reasons, differences have arisen in the approaches to biosphere modelling for repository dose and risk assessment. The BIOMOVS II Reference Biospheres Working Group has developed (a) a recommended methodology for biosphere model development, (b) a structured list of features, events and processes (FEPs) which the model should describe, and (c) an illustrative example of the recommended methodology. The Working Group has successfully tested the Interaction Matrix (or Rock Engineering Systems, RES) approach for developing conceptual models. The BIOMOVS II Working Groups on Reference Biospheres and Complementary Studies have laid the basis for considerable harmonisation in approaches to biosphere modelling of long term radionuclide releases.     

Soil respiration in model plantations under conditions of elevated CO<Subscript>2</Subscript> in the atmosphere (Hokkaido Island,Japan)

The influence of long-term exposure of model plantations at elevated atmospheric CO₂ (550 ppm) on soil respiration under natural conditions has been studied using an automated Free-Air CO₂ Enrichment (FACE) system at the Hokkaido University, Japan. In the course of the experiment, an attempt has been made to simulate the effect of forthcoming climate change on the process of CO₂ emission from different soil types.     

A performance appraisal of size dependent reproduction and reproductive allocation: A case study of two <Emphasis Type="Italic">Inula</Emphasis> species from Kashmir Himalaya

In this study we investigated the size-dependent reproductive pattern of Inula racemosa and I. royleana (Asteraceae) growing at different reaches in the environs of the Kashmir Himalaya. Size effects on reproductive pattern were evaluated by determining the size-dependency of flowering probability and reproductive effort. The results showed that the probability of flowering increased significantly with the size of the plant in all populations, indicating that individuals do not flower until they reach a threshold size and considerable between-site differences were found in the slope and the intercept of the regression between plant size and flower production. In I. racemosa, populations at high altitudes had significantly lower threshold sizes for reproduction and showed sharp increase in flowering probability with plant size, compared to other populations at lower altitudes. However, no pattern in size-dependent flower production was found relative to the altitude in I. royleana, as flower production at some sites, at high altitudes, increased more steeply with plant size than at other sites. For both taxa, reproductive effort decreased allometrically as adults grew, as can be interpreted from the allometric relationship between reproductive and vegetative biomass. Further studies are required to determine whether population differentiation in size-dependent reproductive pattern is maintained by selection.     

Analysis of rodent populations in technogenically transformed areas (with reference to <Emphasis Type="Italic">Apodemus</Emphasis> (<Emphasis Type="Italic">S.</Emphasis>) <Emphasis Type="Italic">uralensis</Emphasis> from the EURT zone)

It is shown that the response of rodent populations to acute and chronic irradiation depends on its functional structure, i.e., on specific features of animals with two alternative types of ontogenetic development. Upon acute irradiation, sexually immature young of the year (animals with the second type of ontogeny) are most radioresistant. Exposure to chronic irradiation, as in the zone of the Eastern Ural Radioactive Trace (EURT), leads to an increase in the proportion of mature young of the year (animals with the first type of ontogeny), which are the most radiosensitive part of the population. The abundance and fecundity of mice in the impact zone are consistently higher than in the background zone, which improves the adaptive potential of the population. The role of species ecological specialization and configuration of the contaminated zone in the formation of migrant rodent population is emphasized. It is concluded that a high migration activity allows the pigmy wood mouse (a radiosensitive species) to avoid long-term radiation exposure.     

Phenotypic diversity in populations of the common blue butterfly <Emphasis Type="Italic">Polyommatus icarus</Emphasis> Rott. As a trend in the ecocenotic strategy of the species

Phenotypic diversity in populations of Polyommatus icarus Rott. (Lepidoptera, Lycaenidae) is regarded as a component of the ecocenotic strategy of the species, trends in which may serve as markers characterizing the state of natural populations.     

Analysis of monitoring data on the Russian desman (<Emphasis Type="Italic">Desmana moschata</Emphasis>) in the Oka state biosphere reserve in relation to the problem of species’ population decline

Data on the effect of environmental factors on the Russian desman population of the Oka Reserve (Ryazan oblast) have been analyzed over the period from 1938 to 2004. Against the background of global climate warming, the number of desmans has decreased by a factor of 2.75 as a consequence of hydrotechnical amelioration in the early 1960s. Prior to amelioration, relatively dry, low-water, and cool years were more favorable for these animals; after amelioration, their demand for more abundant moistening has increased.     

Characteristics of the contact zone of three chromosome races of the common shrew <Emphasis Type="Italic">Sorex araneus</Emphasis> L. (Mammalia) as indices of interpopulation competition

The distribution of three chromosome races (Moscow, Western Dvina, and Seliger) of the common shrew Sorex araneus L. in the zone of contact between their ranges in the Valdai Heights (32°00′ E, 57°00′ N) is discussed. The area co-inhabited by all the three races is extremely small. Representatives of all the three races have been captured on only one trap line, although there are no obstacles to the expansion of shrews. The extremely small area co-inhabited by the three races, as well as the strictly parapatric distribution of the Moscow and Western Dvina races, may be related with competition forms such as avoidance of contacts and separation of ecological niches.