Assessing vulnerability to invasion by nonnative plant species at multiple spatial scales

Basic information on where nonnative plant species have successfully invaded is lacking. We assessed the vulnerability of 22 vegetation types (25 sets of four plots in nine study areas) to nonnative plant invasions in the north–central United States. In general, habitats with high native species richness were more heavily invaded than species-poor habitats, low-elevation areas were more invaded than high-elevation areas, and riparian zones were more invaded than nearby upland sites. For the 100 1000-m² plots (across all vegetation types), 50% of the variation in nonnative species richness was explained by longitude, latitude, native plant species richness, soil total percentage nitrogen, and mean maximum July temperature (n = 100 plots; P < 0.001). At the vegetation-type scale (n = 25 sets of four 1000-m² plots/type), 64% of the variation in nonnative species richness was explained by native plant species richness, elevation, and October to June precipitation (P < 0.001). The foliar cover of nonnative species (log) was strongly positively correlated with the nonnative species richness at the plot scale (r = 0.77, P < 0.001) and vegetation-type scale (r = 0.83, P < 0.001). We concluded that, at the vegetation-type and regional scales in the north–central United States, (1) vegetation types rich in native species are often highly vulnerable to invasion by nonnative plant species; (2) where several nonnative species become established, nonnative species cover can substantially increase; (3) the attributes that maintain high native plant species richness (high light, water, nitrogen, and temperatures) also help maintain nonnative plant species richness; and (4) more care must be taken to preserve native species diversity in highly vulnerable habitats.     

Soil-erosion and runoff prevention by plant covers in a mountainous area (se spain): Implications for sustainable agriculture

In the Mediterranean region the intensities and amounts of soil loss and runoff on sloping land are governed by rainfall pattern and vegetation cover. Over a two-year period (1998–1999), six wild species of aromatic and mellipherous plants (Thymus serpylloides subsp. Gadorensis, Thymus baeticus Boiss, Salvia lavandulifolia Vahl., Santolina rosmarinifolia L., Lavandula stoechas L. and Genista umbellata Poiret) were selected for erosion plots to determine their effectiveness in reducing water erosion on hillslopes of the Sierra Nevada Mountain (SE Spain). The erosion plots (including a bare-soil plot as control), located at 1,345 m in altitude, were 2 m² (2 m × 1 m) in area and had 13% incline. The lowest runoff and soil erosion rates, ranging from 9 to 26 mm yr⁻¹ and from 0.01 to 0.31 Mg ha⁻¹ yr⁻¹, respectively, over the entire study period, were measured under the Thymus serpylloides. Lavandula stoechas L. registered the highest rates among the plant covers tested, runoff ranging from 77 to 127 mm yr⁻¹ and erosion from 1.67 to 3.50 Mg ha⁻¹ yr⁻¹. In the bare-soil plot, runoff ranged from 154 to 210 mm yr⁻¹ and erosion from 4.45 to 7.82 Mg ha⁻¹ yr⁻¹. According to the results, the lowest-growing plant covers (Thymus serpylloides and Salvia lavandulifolia Vahl.) discouraged the soil erosion and runoff more effectively than did the taller and open medium-sized shrubs (Santolina rosmarinifolia L., Genista umbellata Poiret, Thymus baeticus Boiss and Lavandula stoechas L.). Monitoring allowed more direct linkage to be made between plant covers and the prevention of erosion, with implications for sustainable mountain agriculture and environmental protection.     

Earthworm density,casting activities and its impact on canopy soil nutrient profile under different aboveground vegetations

Efforts were made to assess the earthworm’s density and their casting activity under different surface plant stands (Citrus reticulata, Rosa indica, Citrus + Rosa, Tabernaemontana divaricata, Dalbergia sissoo, and Cynodon dactylon) at few natural and planted sites of a semiarid part of Rajasthan, India. The role of earthworm community in nutrient dynamics of different layers of canopy soils was also measured during this study. Results suggested a direct impact of aboveground vegetation cover on soil microclimatic conditions and earthworm density. The maximum earthworm population density and casts production was under mixed plantation, i.e., Citrus + Rosa (119.2 ind. m⁻² and 2,127.27 gm m², respectively), while T. divaricata supported the minimum earthworm density and casting activities. The population density and total casts production was in the order: C. reticulata + R. indica > R. indica > D. sissoo > C. dactylon > C. reticulata > T. divaricata. Earthworm casts and canopy soils (0–10 and 10–20 cm depth) under different plant stand were analyzed for organic C, total N, available P, and exchangeable cations (K⁺, Ca²⁺ and Mg²⁺). Earthworm casts collected under Citrus + Rosa showed the maximum level of organic C, total N, available P, and exchangeable cations (K⁺ and Mg²⁺). It is concluded that resource input by standing plant community directly affects the canopy soil quality and, thereby nutrient level in earthworm casts. This study suggested that aboveground vegetation pattern plays an important role to improve the nutrient level of canopy soils and belowground earthworm activities.     

Agave salmiana Plant Communities in Central Mexico as Affected by Commercial Use

Agave salmiana is a native plant species harvested for the commercial production of mezcal (Agave spirits) in the highlands of central Mexico. The objective of this study was to identify vegetation changes in natural communities where A. salmiana has been differentially harvested for commercial purposes. Three plant community categories were identified in the state of Zacatecas based on their history of A. salmiana utilization: short (less than 10 years of use), moderate (about 25 years), and long (60 or more years). Species cover, composition, and density were evaluated in field surveys by use category. A gradient of vegetation structure of the communities parallels the duration of A. salmiana use. A. salmiana density was greatest (3,125 plants ha⁻¹) in the short-use areas and less (892 plants ha⁻¹) in the moderate-use areas, associated with markedly greater density of shrubs (200%) and Opuntia spp. (50%) in moderate-use areas. The main shrubs were Larrea tridentata, Mimosa biuncifera, Jatropha dioica and Buddleia scordioides while the main Opuntia species were Opuntia leucotricha and Opuntia robusta. A. salmiana density was least (652 plants ha⁻¹) in the long-use areas where shrubs were less abundant but Opuntia spp. density was 25% higher than in moderate-use areas. We suggest that shrubs may increase with moderate use creating an intermediate successional stage that facilitates the establishment of Opuntia spp. Long-term Agave use is generating new plant communities dominated by Opuntia spp. (nopaleras) as a replacement of the original communities dominated by A. salmiana (magueyeras).     

The Effects of Long-Term Grazing Exclosures on Range Plants in the Central Anatolian Region of Turkey

Over the last fifty years, almost half of the steppe rangeland in the Central Anatolian Region of Turkey (CAR) has been converted to cropland without an equivalent reduction in grazing animals. This shift has led to heavy grazing pressure on rangeland vegetation. A study was initiated in June 2003 using 6 multiscale Modified-Whittaker plots to determine differences in plant composition between areas that have not been grazed in 27 years with neighboring grazed plant communities. A total of 113 plant species were identified in the study area with the ungrazed plots containing 32 plants more than the grazed plots. The major species were Astragalus acicularis, Bromus tomentellus, Festuca valesiaca, Genista albida, Globularia orientalis, Poa bulbosa, and Thymus spyleus ssp rosulans. Grazing impacts on forbs were more pronounced than for grasses and shrubs. Based on Jaccard’s index, there was only a 37% similarity of plant species between the two treatments. Our study led to four generalizations about the current grazing regime and long-term exclosures in the steppe rangeland around the study area: (1) exclosures will increase species richness, (2) heavy grazing may have removed some plant species, (3) complete protection from grazing for a prolonged period of time after a long history of grazing disturbance may not lead to an increase in desirable plant species with a concomitant improvement in range condition, and (4) research needs to be conducted to determine how these rangelands can be improved.     

Allelopathic response of <Emphasis Type="Italic">Vetiveria zizanioides</Emphasis> (L.) Nash on members of the family Enterobacteriaceae and <Emphasis Type="Italic">Pseudomonas</Emphasis> spp.

The biological removal of phosphates was carried as a part of treatment strategy. Vetiveria zizanioides (L.) Nash belonging to the family Poaceae was used for biological removal of biostimulants. Vetiver reportedly has mycorrhizal association; besides having potential for removal of PO₄ ⁻³ also showed allelopathic affect on the microorganisms present in the water. In fact after a period of 96 h old roots of this plant have been found to have killing effects on the E. coli, Enterobacter spp. Pseudomonas spp. belonging to the family Enterobacteriaceae. The paper is opening a new face of study.     

Effects of herbage removal on productivity of selected high-sierra meadow community types

We investigated the effects of herbage removal on three subalpine meadow plant communities in the Rock Creek drainage of Sequoia National Park, California, USA. In the xericCarex exserta Mkze. (short-hair sedge) type, annual aboveground productivity averaged 19 g/m² in control plots (clipped once after plant senescence in late September) over a five-year period. Annual aboveground productivity was enhanced about 30%–35% when plots in this community type were clipped more frequently (i.e., additional herbage removal in the early, mid, and late seasons) during each of four treatment years but was reduced by 13%–19% during a fifth (recovery) year in which all but late September clipping was suspended. In a moderately mesicEleocharis pauciflora (Lightf.) Link. (few-flowered spike rush)-Calamagrostis breweri Thurb. (short-hair grass) type, control plot productivity averaged 115 g/m²/yr and was reduced by 20–30% by the additional herbage removal. A more mesicDeschampsia caespitosa (L.) Beauv. (tufted hairgrass)-Carex rostrata Stokes, (beaked sedge) type had the greatest mean above-ground productivity (169 g/m²/yr) but also showed damage (i.e., decrease in productivity by 15%–20%) caused by the additional herbage removal. These data suggest that longterm, intensive herbage removal may be more detrimental to moderately mesic and mesic subalpine meadow community types than to xeric types.     

Effect of spent engine oil on the growth parameters and chlorophyll content of <Emphasis Type="Italic">Corchorus olitorius</Emphasis> Linn

The effect of spent engine oil on the height, leaf number, leaf area, stem girth, chlorophyll, and moisture contents of Corchorus olitorius grown on 0, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 2.0%, and 3.0% (v/w oil/soil) oil-contaminated soil was investigated. The engine oil at all concentrations delayed the germination of C. olitorius by 2 days (compared to control) and there was a general significant reduction in all the growth parameters in plants grown on contaminated soil compared to control plants. The highest leaf area of 26.8 cm² was found in the control plant and least was found in the 0.6% soil (0.11 cm²) after 3 weeks while no values were recorded on the 0.8–3.0% engine-oil-contaminated soil after 5 weeks of experiment. The highest chlorophyll content was also found in the control plant (11.5 mg/l). This showed that spent engine oil has an adverse effect on the growth of C. olitorius plant.     

NITROGEN ACCUMULATION IN A CONSTRUCTED WETLAND FOR DAIRY WASTEWATER TREATMENT1

ABSTRACT: A free water surface (FWS) constructed wetland was installed at a dairy in Glendale, Arizona, to study the potential of such a wetland to remove nitrogen (N) from wastewater. The study objectives were: (1) to determine N removal from the wastewater, and (2) to evaluate N accumulation in soil and plant tissues. The system consisted of eight cells (70 × 9 × 1.5 m) planted with Typha domingensis, Scirpus validus, and Phragmites australis. The four cells in series were lined with plastic, and the four cells in a parallel series were lined with clay. Cells received approximately 180 m³/d of partially treated dairy effluent. Plant tissues and soil samples were collected above and below ground from 24 locations during one year. Total N removal from wastewater was about 17 percent. Clay‐lined cells accumulated more N in the soil and less N in plant biomass compared with plastic lined cells. Plant biomass accounted for approximately 60 percent of total N accumulated in cells with dense plant communities. Ninety percent of accumulated soil N was organic. Total N accumulated in soil reached a maximum (1,100 mg/kg) eight months after the introduction of wastewater.     

A PRELIMINARY QUANTIFICATION OF THE IMPACTS OF ASPEN TO CONIFER SUCCESSION ON WATER YIELD - I. HEAT PULSE METHODOLOGY FOR MODEL CALIBRATION1

ABSTRACT: Twenty-six aspen (Populus tremuloides Michx.), 20 subalpine fir (Abies lasiocarps (Hook.) Nutt.), and 20 Engelmann spruce (Pices engelmanil (Parry) Engelm.) of various sizes were cut under water and suspended in permanent reserviors at a northern Utah site. The reservoirs were asealed so that all water loss was due to consumption by the trees. Sap velocities, as computed from heat pulse velocities, were related to conducting areas of the tree trunks. Computed transpiration volumes were then correlated with actual water losses from the reservoirs. Coefficients of determination (R²) of 0.87, 0.86, and 0.82 were obtained for the fir, aspen, and sprucs, respectively. Reservoir water loss for each species for each season was then used to adjust a plant activity index for computing transpiration within ASPCON, a model describing the hydrology of aspen to conifer succession. The plant activity index reflects the variation in the capability of a plant community to transpire water over the year. Assumptions and limitations of the heat pulse velocity technique are also outlined.     

Evaluation of tidal marsh restoration: Comparison of selected macroinvertebrate populations on a restored impounded valley marsh and an unimpounded valley marsh within the same salt marsh system in Connecticut,USA

Macroinvertebrates were examined on an impounded valley marsh in Stonington, Connecticut, that has changed from aTypha-dominated system to one with typical salt-marsh vegetation during 13 years following the reintroduction of tidal exchange. Animal populations on this restored impounded marsh were evaluated by comparing them with populations on a nearby unimpounded valley marsh of roughly the same size. Populations of the high marsh snail,Melampus bidentatus Say, were quantitatively sampled along transects that extended from the water-marsh edge to the upland; those of the ribbed mussel,Geukensia demissa Dillwyn, were sampled in low marsh areas on transects along the banks of creeks and mosquito ditches. The occurrence of other marsh invertebrates also was documented, but their abundance was not measured. The mean density ofMelampus was 332±39.6 SE/m² on the restored impounded marsh and 712±56.0 SE/m² on the unimpounded marsh. However, since snails were larger on the restored impounded marsh, the difference in snail biomass was less pronounced than the difference in snail density. MeanMelampus biomass was 4.96±0.52 SE g dry wt/m² on the restored impounded marsh and 6.96±0.52 SE g dry wt/m² on the unimpounded marsh. On the two marshes, snail density and biomass varied in relation to plant cover and other factors. The density and biomass ofGeukensia at the edge of the marsh were comparable on the restored impounded and unimpounded marshes. Mean mussel densities ranged from 80 to 240/m² and mean mussel biomass varied from 24.8–64.8 g dry wt/m² in different low marsh areas. In contrast, below the impoundment dike, meanGeukensia density was 1100±96.4 SE/m² and meanGeukensia biomass was 303.6±33.28 SE g dry wt/m². A consideration of all available evidence leads to the conclusion that the impounded marsh is in an advanced phase of restoration.     

Comparison of biosorbents with inorganic sorbents for removing copper(II) from aqueous solutions

In comparison with several other reported inorganic sorbents, Camellia tree leaf and primary sludge obtained from a settling tank as a pretreatment to the activated sludge system in a Hong Kong sewage treatment plant were evaluated for removing Cu(II) from aqueous solutions. Experimental data were modeled by the Langmuir isotherm equation to estimate the maximum sorption capacity (q_max). Results show that, at pH 5.6, biosorbents, Camellia tree leaf and primary sludge in particular, exert higher sorption capacities (q_max > 40 mg g⁻¹) than inorganic sorbents, Na-montmorillonite (q_max = 33.3 mg g⁻¹), fly ash (q_max = 18.8 mg g⁻¹), and goethite powder (10.3 mg g⁻¹). Furthermore, a pseudo second-order kinetic model was found to properly describe the experimental data for both bio- and inorganic sorbents. Sorption of Cu(II) on the Camellia tree leaf and primary sludge were much faster than that on the inorganic sorbents. In addition, desorption tests revealed that the desorption capacities of the two biomaterials are higher than the other selected materials; and much more Cu(II) can be retrieved from the Cu(II)-loaded biosorbents. Finally, increasing solution pH was found to greatly increase q_max and accelerate sorption processes.     

Microalgae‐based biodiesel production in open raceway ponds using coal thermal flue gas: A case of West Bengal,India

India is one of the most populous countries and is the third largest greenhouse gas–emitting nation. Energy security is a serious issue for India as it relies heavily on fossil fuel imports. Biodiesel production using microalgae as feedstock can address both of these issues. In this study, the technical feasibility of microalgae‐based biodiesel production is carried out for a coal thermal power plant (i.e., Budge Budge Thermal Station) in the state of West Bengal, India, using a generic methodology. An oleaginous microalgae species that is tolerant toward flue gas was identified (i.e., Nannochloropsis sp). A 75‐acre open raceway microalgae production plant was designed keeping the costs, energy demand, and CO₂ emissions low. The open raceway pond can use 38 tons of CO₂, produce 19 tons of algal biomass, and treat 9320 m³ of wastewater per acre annually.     

Possibility of converting indigenous Salvadora persica L. seed oil into biodiesel in Pakistan

In this research study, biodiesel has been successfully produced from vegetable seed oil of an indigenous plant Salvadora persica L. that meets the international biodiesel standard (ASTM D6751). The biodiesel yield was 1.57 g/5 g (31.4% by weight) and the in-situ transesterification ester content conversion was 97.7%. The produced biodiesel density was 0.894 g/mL, its kinematic viscosity 5.51 mm²/s, HHV 35.26 MJ/kg, flash point 210°C, cetane no. 61, and sulfur content 0.0844%. Thermal analysis of the biodiesel showed that 97% weight loss was achieved at 595°C with total oxidation of the biodiesel. The production energy efficiency was 0.46% with a lab scale setup, assuming the volume fraction ratio (volume of the sample/total volume of the equipment used). The results revealed that single-step in-situ transesterification method is suitable for the production of biodiesel from S. persica seed oil.     

Evaluation of the European Space Agency Climate Change Initiative Soil Moisture Product over China Using Variance Reduction Factor

In this study, we evaluated the European Space Agency Climate Change Initiative soil moisture product v02.1 (ESA CCI SM v02.1) using in situ observations collected at 547 stations in China from 1991 to 2013. A conventional validation was first conducted, and the triple collocation errors of ESA CCI SM and the European Centre for Medium Range Weather Forecasting reanalysis data were approximately 0.053 and 0.050 m³/m³, respectively. To obtain more reliable validation results, the average soil moisture of the in situ observations per ESA CCI SM pixel was also used as the validation sites. Variance reduction factor (VRF) was adopted to quantify the accuracy of the soil moisture validation sites, and the average VRF was estimated at 4.88%. The validation results were enhanced by excluding validation sites with VRF errors greater than 5% from the statistical analysis. Although the ESA CCI SM underestimated the in situ observations with a Bias of 0.05 m³/m³, a moderately high correlation coefficient of 0.44 and a relatively small unbiased root‐mean‐square difference of 0.05 m³/m³ were observed. This study provides information on the utilization of ESA CCI SM for ecological protection, climate change, and hydrological forecasting. It also suggests the adoption of VRF for future error corrections of satellite‐based products.     

How Green is Too Green? Public Opinion of What Constitutes Undesirable Algae Levels in Streams1

Abstract: A public opinion survey was carried out in Montana to ascertain if the public identifies a level of benthic (bottom‐attached) river and stream algae that is undesirable for recreation. The survey had two parts; an On‐River survey and a By‐Mail survey. The On‐River survey was conducted via 44 trips randomly scheduled throughout the state during which recreators were interviewed in‐person at the stream. Selection of stream segments and survey dates/times was based on known, statewide recreational use patterns. By‐Mail survey forms were sent to 2,000 individuals randomly selected from Montana’s Centralized Voter File (CVF) available from the Montana Secretary of State. The CVF was current through 2004 and represented over 85% of the state’s eligible voting population. In both surveys, eight randomly ordered photographs depicting varying levels of stream benthic algae were presented, and participants were asked if the algae level shown was desirable or undesirable for recreation. Survey form design, selection of photographs, and pretesting followed acceptable protocols that limited unintentional bias through survey execution. There were 433 returned forms (389 complete) for the By‐Mail survey, while the On‐River survey documented 563 interviews. In both surveys, as benthic algal chlorophyll a (Chl a) levels increased, desirability for recreation decreased. (Other measures of benthic algae biomass are presented as well.) For the public majority, mean benthic Chl a levels ≥200 mg/m² were determined to be undesirable for recreation, whereas mean levels ≤150 mg Chl a/m² were found to be desirable. Error rates were within the survey’s statistical design criteria (≤5%). The largest potential error source was nonresponse in the By‐Mail survey; however, the population represented by nonrespondents would have to exhibit profoundly different perceptions of river and stream algae to meaningfully alter the results. Results support earlier work in the literature suggesting 150 mg Chl a/m² represents a benthic algae nuisance threshold.     

Release,Dispersion, and Resuspension of Escherichia coli From Direct Fecal Deposits Under Controlled Flows1

Abstract: Water‐quality standards have been placed on fecal indicator organisms such as Escherichia coli in an attempt to limit the concentrations in water bodies. Cattle can be a significant source of bacteria to water systems, particularly when they are allowed direct access to streams. A flume study was conducted to quantify the effect and understand the transport of E. coli from directly deposited cattle manure. Five steady‐state flows, ranging from 0.00683 to 0.0176 m³/s, were studied and loads from a single cowpie exceeded the U.S. Environmental Protection Agency’s recommended water‐quality standards (235 CFU/100 ml) at each flow over the hour study period. Average E. coli concentrations ranged from 10² to 10⁵ CFU/100 ml over the hour sampling period for all flows. High spatial variations in E. coli concentrations were often seen at each sampling time, with higher concentrations typically at the bottom of the flume. E. coli resuspension was initially greater at 0.5 min after deposition, for the lowest flow (10⁵ CFU/m²/s); however, resuspension rates became similar over time, on the order of 10³ CFU/m²/s. This study demonstrates that the concentrations of E. coli can vary over the water column, and therefore grab samples may inaccurately measure bacteria concentrations and loads in streams. In addition, resuspension rates were often high, so the incorporation of this process into water‐quality models is important for bacteria prediction.     

Effects of spent engine oil on the growth parameters, chlorophyll and protein levels of Amaranthus hybridus L.

The susceptibility of Amaranthus hybridus L. seedlings to spent engine oil was investigated in soil supplemented with concentrations of oil ranging from 1–5 percent v/w. Parameters considered were relative growth rate (RGR), leaf area ratio (LAR), whole plant height, leaf area, leaf number, chlorophyll and protein levels. A relationship was found to exist between the inhibitory effects and the treatment concentrations. After seventy days growth in the treated soils, the mean height and leaf area of plants in soils treated with 5 percent spent engine oil were 27.0±1.25 cm and 5.63±0.36 cm². These were significantly different (at p=0.05) from the respective values of 41.4±0.8 cm and 13.44±0.22 cm² for the control plants. Levels of total chlorophyll (per gram fresh weight of leaves) and protein (per gram dry weight of whole plant) were higher in the control plants compared with those grown in oil treated soil. Results obtained from the growth analysis showed the inhibitory effects of spent engine oil on Amaranthus hybridus L.     

The Use of Carbon and Nitrogen Isotopes to Study Watershed Erosion Processes1

Abstract:  Tracer studies are needed to better understand watershed soil erosion and calibrate watershed erosion models. For the first time, stable nitrogen and carbon isotopes (δ¹⁵N and δ¹³C) and the carbon to nitrogen atomic ratio (C/N) natural tracers are used to investigate temporal and spatial variability of erosion processes within a sub‐watershed. Temporal variability was assessed by comparing δ¹⁵N, δ¹³C, and C/N of eroded‐soils from a non‐equilibrium erosion event immediately following freezing and thawing of surface soils with two erosion events characterized by equilibrium conditions with erosion downcutting. Spatial variability was assessed for the equilibrium events by using the δ¹⁵N and δ¹³C signatures of eroded‐soils to measure the fraction of eroded‐soil derived from rill/interrill erosion on upland hillslopes as compared to headcut erosion on floodplains. In order to perform this study, a number of tasks were carried out including: (1) sampling source‐soils from upland hillslopes and floodplains, (2) sampling eroded‐soils with an in situ trap in the stream of the sub‐watershed, (3) isotopic and elemental analysis of the samples using isotope ratio mass spectrometry, (4) fractioning eroded‐soil to its upland rill/interrill and floodplain headcut end‐members using an unmixing model within a Bayesian Markov Chain Monte Carlo framework, and (5) evaluating tracer unmixing model results by comparison with process‐based erosion prediction models for rill/interrill and headcut erosion processes. Results showed that finer soil particles eroded during the non‐equilibrium event were enriched in δ¹⁵N and δ¹³C tracers and depleted in C/N tracer relative to coarser soil particles eroded during the equilibrium events. Correlation of tracer signature with soil particle size was explainable based on known biogeochemical processes. δ¹⁵N and δ¹³C were also able to distinguish between upland rill/interrill erosion and floodplain headcut erosion, which was due to different plant cover at the erosion sources. Results from the tracer unmixing model highlighted future needs for coupling rill/interrill and headcut erosion prediction models.     

Evidential role of municipal solid waste and liquid effluent on environment and public health

Cities in Bangladesh produce large amounts of solid waste (SW) through various human activities which severely pollutes our native environment. As a result, SW pollutes the three basic environmental elements (air, water, and soil) by increasing pathogenic microbial load, which might be hazardous to public health directly or indirectly. In this study, we conducted 30 samples (i.e., soil, water, and air) collected from areas where municipal solid wastes are dumped (Tangail Sadar Upazila, Bangladesh). All the samples were analyzed to assess bacteriological quality for presumptive viable and coliform count using different agar media. We performed serial dilution 10⁻³–10⁻¹⁰ times for soil and water samples, and the diluted samples were spread on Mac-Conkey agar and nutrient agar plates. For the air sample, the sterile media containing petri-dish was placed adjacent to the dumpsite of the municipal waste and kept for an hour. Then all the samples were incubated at 37°C overnight for total viable count (TVC) and total coliform count (TCC). Biochemical tests and PCR were performed for the identification of these microorganisms. The antibiogram study was performed to reveal their (identified bacteria) susceptibility against clinically used antibiotics according to the standard disk diffusion technique. The highest bacterial loads were found in the air: TVC 3.273 × 10³ and TCC 1.059 × 10³ CFU/plate; tube-well water: TVC 8.609 × 10³, and TCC 8.317 × 10³ CFU/mL; in surface water: TVC 6.24 × 10¹³ CFU/mL and TCC 2.2 × 10¹² CFU/mL; in soil: TVC 2.88 × 10¹¹ and TCC 1.02 × 10¹¹ CFU/g, respectively. Microbes from SW can be transmitted through air, dust particles, or flies, and here we found an average of 1120 microbes spread over 63.61 cm² area per hour. Eight bacterial isolates (Pseudomonas spp., Klebsiella spp., E. coli, Proteus spp., V. cholera, Salmonella spp., Shigella spp., and Vibrio spp.) were identified by the biochemical test. Among them, E. coli and Shigella spp. were further ensured by PCR targeting bfpA and ipaH genes. Antibiotic susceptibility test results showed that E. coli isolates were highly resistant to erythromycin (80%); Shigella spp. were resistant to nalidixic acid (90%), whereas Salmonella spp. was found resistant to kanamycin (90%). Vibrio spp. were also resistant to azithromycin (80%) and erythromycin (80%), which should be a great concern for us. A semi-structured survey revealed that 63% of respondents suffered from different clinical conditions (intestinal diseases) due to SW pollution. So, steps should be taken to improve the proper management and disposal of solid waste and liquid effluent to save our environment and public health.