首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 31 毫秒
1.
The effect of irradiance, prey concentration and pH on the growth and grazing responses of the mixotrophic prymnesiophyte Chrysochromulina ericina under N-and P-replete conditions was studied using the pedinophyte Marsupiomonas pelliculata as prey. The two organisms were inoculated in monocultures and in mixed cultures at different predator: prey ratios at three irradiances and allowed to grow for 4–7 days. All cultures were non-axenic. Algal densities and pH were monitored throughout the experiments and growth and grazing rates were measured. An increase in growth of C. ericina cultures at irradiances of 25 and 70 μmol photons m−2 s−1 was observed after the addition of prey, while growth of C. ericina cultures at the high irradiance (150 μmol photons m−2 s−1) was unaffected by the addition of prey. However, although the growth of C. ericina increased at low irradiance (25 μmol photons m−2 s−1), it did not reach the same level as monocultures at the high irradiance (150 μmol photons m−2 s−1), suggesting that phagotrophy can only partly replace photosynthesis in C. ericina. Maximum growth rates of C. ericina at irradiances of 25 and 70 μmol photons m−2 s−1 were obtained at concentrations of > 0.15–0.3×105 M. pelliculata ml−1, corresponding to 50–100 μg C 1−1. Ingestion of M. pelliculata cells by C. ericina did not generally follow Michaelis—Menten kinetics. Deviation from the expected saturation kinetics was especially pronounced at irradiances of 70 and 150 μmol photons m−2 s−1. At these irradiances ingestion of M. pelliculata cells by C. ericina decreased at high concentrations of M. pelliculata, indicating an increased uptake of bacterial prey in these cultures. The growth rate of C. ericina was affected in both monocultures and in mixed cultures when pH increased above 8.6, and growth stopped around pH 9. The prey alga M. pelliculata tolerated high pH better and, consequently, took over in the mixed cultures when pH exceeded 9. The ecological significance of mixotrophy in the genus Chrysochromulina is discussed. Published online: 4 July 2002  相似文献   

2.
The influence of naturally occurring uraniferous black shales on cadmium, molybdenum and selenium concentrations in soils and plants is examined. The possible implications of element concentrations to animal and human health are considered for the Deog-Pyoung area. Geochemical surveys have been undertaken within 13 river tributary valleys in the area underlain by uraniferous black shales and black slates or grey chlorite schists. Sampling of rocks, soils and plants has been carried out along transect lines within each valley. Samples were analysed for trace elements by Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) and for uranium by Neutron Activation Analysis (NAA). Soil pH, cation exchange capacity, loss on ignition and particle size distribution have been measured for selected samples. Average trace element concentrations of the Okchon uraniferous black shales were 6.3 μg g−1 Cd, 136 μg g−1 Mo and 8.6 μg g−1 Se. Soils derived from these rocks tend to reflect their extreme geochemical composition. Trace element concentrations in alluvial soils derived in part from these black shales averaged 1.2 μg g−1 Cd, 20 μg g−1 Mo and 1.5 μg g−1 Se. Trace element concentrations in plants were found to be influenced by those of soils. Cadmium accumulated in tobacco leaves up to 46 μg g−1 (D.M.) and leafy plants such as lettuce contain up to 0.5 μg g−1 Se (D.M.). In addition to total concentrations in soils, soil pH is a major factor influencing uptake of Mo into crop plants and soil texture for Se. Concentrations of trace elements in plants also varied between plant species. The relative concentrations of Cd were found to vary in the order tobacco > lettuce > red pepper > rice grain. Elevated concentrations of Cd in crop plants and in tobacco may possibly have deleterious effects on human health in this area. The low Cu:Mo ratio in rice stalk of 2.65:1 may be associated with disturbed Cu metabolism in ruminant animals which regularly consume this material.  相似文献   

3.
The kinetics of ammonium assimilation was investigated in Ulva pertusa (Chlorophyceae, Ulvales) from northeastern New Zealand. Ammonium assimilation exhibited Michaelis–Menten kinetics with a maximum rate of assimilation (V max) of 54 ± 5 μmol g−1 dry weight h−1 and half-saturation constant (K m) of 23 ± 8 μM. In contrast, values for ammonium uptake were considerably higher with a V max of 316 ± 59 μmol g−1 dry weight h−1 and K m of 135 ± 46 μM. At environmentally relevant ammonium concentrations (5 μM), assimilation accounted for most (70%) of the ammonium taken up. Darkness decreased the maximum rate of ammonium assimilation by 83%. We investigated the hypothesis that rates of biosynthetic processes are greater in the early part of the day in Ulva. Consistent with this hypothesis, the maximum rate of ammonium assimilation in U. pertusa peaked in the morning and coincided with low levels of the photosynthetic product sucrose, which peaked in the afternoon. There was a diurnal cycle in the rate of ammonium uptake and assimilation in light and dark, but the amplitude was much greater for assimilation than uptake. Moreover, our data suggest that net ammonium assimilation only occurs during the day in U. pertusa. We suggest that two major roles for diurnal cycles are minimisation of interspecific competition for resources and metabolic costs.  相似文献   

4.
Movement rate, oxygen consumption, and respiratory tree ammonium concentration were measured in situ in the holothurians Pearsonothuria graeffei and Holothuria edulis in the Agan-an Marine Reserve, Sibulan, Philippines (9°20′30″N, 123°18′31″E). Measurements were made both day and night for both species during April–July 2005. P. graeffei had significantly higher movement rate during the day than at night (1.14 and 0.27 m h−1, respectively; three-way ANOVA, P < 0.05) while H. edulis had higher movement rate at night compared to the day (0.83 and 0.07 m h−1, respectively), spending the daylight hours sheltering under coral. More than 80% of H. edulis had movement rate of zero during the day. Oxygen consumption of P. graeffei was significantly higher during the day than at night (1.61 and 0.83 μmol O2 g−1 h−1, respectively; two-way ANCOVA, P < 0.05), but the reduction at night was not as pronounced as the reduction in movement. H. edulis had a 75% reduction in oxygen consumption during the day compared to night (0.51 and 1.96 μmol O2 g−1 h−1, respectively), matching this species’ reduced movement rates during the day. Ammonium concentration in water withdrawn from the respiratory trees of P. graeffei during the day (12.0 μM) was three times higher than in respiratory tree water sampled at night (4.3 μM) and 15 times higher than ambient seawater (0.8 μM; three-way ANOVA, P < 0.05). Ammonium concentration in the respiratory tree water of H. edulis was six times higher at night (14.6 μM) than during the day (2.2 μM) and 16 times higher than that of ambient seawater (0.9 μM). Even though H. edulis and P. graeffei are found within the same coral reef environment, they may affect different substrates and reef organisms due to their different habitats and distinct but opposite diel cycles.  相似文献   

5.
Ammonium concentrations of ∼1 M are commonly cited as being the threshold for inhibition of NO3 uptake, but the applicability of this threshold to phytoplankton from different taxonomic classes has rarely been examined. Additionally, little is known about the influence of environmental variables (e.g. growth temperature) on the interaction between ambient NH4 + and NO3 uptake. Four species of estuarine phytoplankton, two diatom [Chaetoceros sp., and Thalassiosira weissflogii (Grunow) Fryxell et Hasle] and two dinoflagellate [Prorocentrum minimum (Pavillard) Schiller, and Gyrodinium uncatenum Hulburt], were grown on NO3 at several different temperatures (4, 10, 15, or 20 °C), and the impact of NH4 + additions on NO3 uptake/assimilation (non-TCA-extracted) and assimilation (TCA-extracted) was assessed. For all species at all temperatures, NO3 uptake/assimilation and assimilation rates decreased in a roughly exponential manner with increasing NH4 + concentrations but were not completely inhibited even at elevated NH4 + concentrations of 200 μM. Estimated half-inhibition concentrations (K i) were significantly greater in the diatom species (mean ± SE; 2.70 ± 0.67 μM) than in the dinoflagellate species (1.26 ± 0.55 μM). Half-inhibition constants were positively related to temperature-limited relative growth rate although not significantly. The observed inhibition of NO3 uptake and assimilation, as a percentage of NO3 uptake in the absence of NH4 +, averaged about 80% and ranged from 49 to 100%. For all species, a significant (P < 0.001) positive correlation was found between percent inhibition of NO3 assimilation and temperature-limited relative growth rate. Two experiments on Chesapeake Bay phytoplankton during an April 1998 diatom bloom showed that in short-term (∼1 h) temperature manipulation experiments, percent inhibition of NO3 uptake/assimilation was also positively related (P = 0.05) to experimental temperature. The observed relationships between temperature-limited relative growth rate and percent inhibition of NO3 assimilation rates for the species tested suggest that at the enzyme level, the inhibitory mechanism of NO3 assimilation is similar among species, but at the whole cell level may be regulated by species-specific differences in the accumulation of internal metabolites. These findings add not only to our understanding of species-specific variability and the role of growth temperature, but also provide additional data with which to evaluate current models of NH4 + and NO3 interactions. Received: 31 August 1998 / Accepted: 7 December 1998  相似文献   

6.
The relative contribution of dissolved nitrogen (ammonium and dissolved free amino acids DFAAs) to the nitrogen budget of the reef-building coral Pocillopora damicornis was assessed for colonies growing on control and ammonium-enriched reefs at One Tree Island (southern Great Barrier Reef) during the ENCORE (Enrichment of Nutrient on Coral Reef; 1993 to 1996) project. P. damicornis acquired ammonium at rates of between 5.1 and 91.8 nmol N cm−2 h−1 which were not affected by nutrient treatment except in the case of one morph. In this case, uptake rates decreased from 80.5 to 42.8 nmol cm−2 h−1 (P < 0.05) on exposure to elevated ammonium over 12 mo. The presence or absence of light during measurement did not influence the uptake of ammonium ions. Nitrogen budgets revealed that the uptake of ammonium from concentrations of 0.11 to 0.13 μM could completely satisfy the demand of growing P. damicornis for new nitrogen. P. damicornis also took up DFAAs at rates ranging from 4.9 to 9.8 nmol N cm−2 h−1. These rates were higher in the dark than in the light (9.0 vs 5.1 nmol m−2 h−1, P < 0.001). Uptake rates were highest for the amino acids serine, arginine and alanine, and lowest for tyrosine. DFAA concentrations within the ENCORE microatolls that received ammonium were undetectable, whereas they ranged up to 100 nM within the control microatolls. The contribution of DFAAs to the nitrogen budget of P. damicornis constituted only a small fraction of the nitrogen potentially contributed by ammonium under field conditions. Even at the highest field concentrations measured during this study, DFAAs could contribute only ≃11.3% of the nitrogen demand of P.␣damicornis. This contribution, however, may be an important source of nitrogen when other sources such as ammonium are scarce or during periods when high concentrations of DFAAs become sporadically available (e.g. cell breakage during fish-grazing). Received: 22 April 1998 / Accepted: 3 November 1998  相似文献   

7.
Seasonal variations and the effect of reproductive development on resource acquisition by two intertidal fucoid species, the iteroparous Fucus serratus L. and the semelparous Himanthalia elongata (L.) S. F. Gray were examined. The oxygen-exchange characteristics of vegetative apical tissue of both non-fertile and fertile plants and receptacle tissue were compared at monthly intervals throughout reproductive development. Respiratory rates in non-fertile F. serratus varied seasonally between 1.5 and 8.0 μmol g−1 fresh wt h−1; in fertile plants the receptacle had a significantly lower respiratory rate than the vegetative tissue. The respiratory rate of the vegetative button of fertile H. elongata displayed less seasonal variation and was lower than that of the receptacle, which varied from a maximum of 9.5 μmol g−1 fresh wt h−1 at receptacle initiation in October to a minimum of 2.0 μmol g−1 fresh wt h−1 in February. The maximum photosynthetic rate (P max) of non-fertile plants of both species did not vary in a distinct seasonal manner (∼60 μmol g−1 fresh wt h−1 for F. serratus and ∼12 μmol g−1 fresh wt h−1 for H. elongata). In fertile plants, the P max of the receptacle tissue was (∼50% lower in F. serratus, and at its peak three times higher in H. elongata, than that of vegetative tissue. The stable carbon-isotope ratio (δ13C) did not differ between different tissue types in F. serratus, but values did vary seasonally, being less negative in the summer than in the winter (−13.5‰ compared to −18‰). The receptacle tissue of H. elongata also displayed a distinct seasonal variation in δ13C values (−12‰ in summer, −16‰ in winter), whilst the δ13C of the vegetative button did not vary seasonally. The rate of uptake of inorganic nitrogen by the vegetative thallus was lower in H. elongata than in F. serratus. The receptacle tissue of F. serratus had lower uptake rates than the vegetative tissue, whilst the uptake rate by H. elongata receptacle tissue was higher than that of the vegetative button. Received: 14 March 1997 / Accepted: 22 April 1997  相似文献   

8.
In situ measurements of seagrass photosynthesis in relation to inorganic carbon (Ci) availability, increased pH and an inhibitor of extracellular carbonic anhydrase were made using an underwater pulse amplitude modulated (PAM) fluorometer. By combining the instrument with a specially designed Perspex chamber, we were able to alter the water surrounding a leaf without removing it from the growing plant. Responses to Ci within the chamber showed that subtidal plants of the seagrasses Cymodocea serrulata and Halophila ovalis had photosynthetic rates that were limited by the ambient Ci concentration depending on the irradiance that was available during short-term photosynthesis–irradiance trials. Relative electron transport rates (RETRs) at light saturation (up to 500 μ mol photons m−2 s−1) increased by 66–100% when the Ci concentration was increased from ca. 2.2 to 6.2 mM. On the other hand, intertidal plants of the same species exhibited a much lesser limitation of photosynthesis by Ci at any irradiance (up to 1500 μ mol photons m−2 s−1). Both species were able to use HCO 3 efficiently, and there was stronger evidence for direct uptake of HCO 3 rather than extracellular dehydration of HCO 3 to CO2 prior to Ci uptake. Subtidally, H. ovalis and C. serrulata grew to 10 and 12 m, respectively, where ambient irradiances were approximately 16 and 11% of those at the surface. Maximum RETRs (at light saturation) were lower for these deep-growing plants than for the intertidally growing ones. For both species, the onset of light saturation of photosynthesis (E k) occurred at approximately 100 μ mol photons m−2 s−1 for the deep water populations, which was four and two times lower than for the shallow populations of C. serrulata and H. ovalis, respectively. This, and the differences in maximal photosynthetic rates (RETR max), reflects an acclimation of the deep-growing populations to the lower light environment. The results presented here show that photosynthesis, as measured in situ, was limited by the availability of Ci for the deeper growing plants in Zanzibar, while the intertidally growing plants photosynthesised at close to Ci saturation. The latter result is contrary to previous conclusions regarding Ci limitations for these intertidal plants, and, in general, our findings highlight the need for performing similar experiments in situ rather than under laboratory conditions. Received: 4 April 2000 / Accepted: 31 August 2000  相似文献   

9.
Great scallop, Pecten maximus, and blue mussel, Mytilus edulis, clearance rate (CR) responses to low natural seston concentrations were investigated in the laboratory to study (1) short-term CR variations in individual bivalves exposed to a single low seston diet, and (2) seasonal variations in average CR responses of bivalve cohorts to natural environmental variations. On a short temporal scale, mean CR response of both species to 0.06 μg L−1 chlorophyll a (Chl a) and 0.23 mg L−1 suspended particulate matter (SPM) remained constant despite large intra-individual fluctuations in CR. In the seasonal study, cohorts of each species were exposed to four seston treatments consisting of ambient and diluted natural seston that ranged in mean concentration from 0.15 to 0.43 mg L−1 SPM, 0.01 to 0.88 μg L−1 Chl a, 36 to 131 μg L−1 particulate organic carbon and 0.019 to 0.330 mm3 L−1 particle volume. Although food abundance in all treatments was low, the nutritional quality of the seston was relatively high (e.g., mean particulate organic content ranged from 68 to 75%). Under these low seston conditions, a high percentage of P. maximus (81–98%) and M. edulis (67–97%) actively cleared particles at mean rates between 9 and 12 and between 4 and 6 L g−1 h−1, respectively. For both species, minimum mean CR values were obtained for animals exposed to the lowest seston concentrations. Within treatments, P. maximus showed a greater degree of seasonality in CR than M. edulis, which fed at a relatively constant rate despite seasonal changes in food and temperature. P. maximus showed a non-linear CR response to increasing Chl a levels, with rates increasing to a maximum at approximately 0.4 μg L−1 Chl a and then decreasing as food quantity continued to increase. Mean CR of M. edulis also peaked at a similar concentration, but remained high and stable as the food supply continued to increase and as temperatures varied between 4.6 and 19.6°C. The results show that P. maximus and M. edulis from a low seston environment, do not stop suspension-feeding at very low seston quantities; a result that contradicts previous conclusions on the suspension-feeding behavior of bivalve mollusks and which is pertinent to interpreting the biogeographic distribution of bivalve mollusks and site suitability for aquaculture.  相似文献   

10.
To investigate copepod nauplii ingestion rates on phytoplankton, we have adapted the traditional gut fluorescence technique as it can be used with lower gut pigment concentrations. With the improved technique, laboratory experiments were performed to estimate functional responses for nauplii of Calanus helgolandicus and Centropages typicus. Nauplii were raised from eggs to copepodites and the experiments were performed with stages NIV-NV. Gut evacuation rates and ingestion rates were measured on Isochrysis galbana at different concentrations. Specific ingestion rates ranged between 0.038–0.244 μg C μg−1 nauplii C d−1 for C. typicus and 0.041–1.412 μg C μg−1 nauplii C d−1 for C. helgolandicus. Both species showed a type III functional response, reaching a saturation concentration at around 600 μgC l−1 for C. typicus and 800 μgC l−1 for C. helgolandicus. An erratum to this article can be found at  相似文献   

11.
The toxicity of fenitrothion was determined in larvae (nauplii, Zoeae 1 to 3, Mysis 1 to 3), postlarvae (PL stages) and juvenile shrimp (Penaeus japonicus Bate), in two media, seawater (SW) and diluted seawater (DSW) (1100 and 550 mosM kg−1, ≃ 37 and 19‰ S). The effects of fenitrothion on the osmoregulatory capacities (OC) of juveniles were recorded. A gill and epipodite histopathological study was also conducted. For larvae in seawater, 24 and 48 h LC50s ranged from 32.9 μg l−1 (Zoeae 2) to 10.7 μg l−1 (Mysis 3), and from 3.9 μg l−1 (Zoeae 3) to 2.0 μg l−1 (Mysis 3), respectively; 48 and 96 h  LC50s in postlarvae (PL) at the same salinity ranged from 1.8 μg l−1 (PL1) to 0.6 μg l−1 (PL5), and from 0.3 μg l−1 (PL7) to 0.4 μg l−1 (PL15). In juveniles, 96 h LC50s were 0.8 μg l−1 in seawater and 1.5 μg l−1 in diluted seawater. From hatching to juvenile stages, the overall trend was a rapid decrease (from nauplii to PL5–PL7) followed by a slight increase (from PL7 to PL15 and juveniles) in the shrimp's ability to tolerate the insecticide. In juveniles kept in seawater and in diluted seawater, fenitrothion decreased the osmoregulatory capacity (OC = difference between the hemolymph osmotic pressure and the osmotic pressure of the medium) at both lethal and sublethal concentrations. This effect was time- and dose-dependent. In SW, the decrease in hypo-OC was ˜ 25% at sublethal concentrations and ˜ 35% at the 96 h LC50. In DSW, the decrease in hyper-OC was ˜ 10 to 15% at sublethal concentrations. In SW, shrimp were able to recover their OC in less than 48 h when transferred to water free of pesticide. In DSW, recovery at 48 h was only possible after exposure to the lowest tested sublethal concentration. Haemocytic congestions (thrombosis) of the gills, lamellae necrosis and other alterations of gills and epipodites (breakage of the cuticle, reduction of the hemolymph lacunae) were noted in juveniles exposed to lethal and sublethal concentrations of fenitrothion. Received: 7 October 1996 / Accepted: 13 November 1996  相似文献   

12.
Balanus amphitrite, an acorn barnacle, is distinctly euryhaline, eurythermal and a dominant fouling organism found in warm and temperate waters throughout the world. In this study, the influence of temperature and food concentration on the reproductive biology of this species collected from a tropical habitat was evaluated. Adult barnacles were maintained at 20, 25 and 30°C temperatures at different concentrations of food (50, 100, 150 and 200 Artemia ind−1 day−1). In this previously believed obligatory cross-fertilizing hermaphrodite, self-fertilization was observed. The rise in temperature from 20 to 30°C resulted in a longer interbreeding interval (6–7 days, 200 Artemia ind−1 day−1; 11–13 days, 50 Artemia ind−1 day−1). Computed carbon gained through feeding during the interbreeding interval indicated an inverse relationship to the temperature. At 20°C, although a greater amount of carbon was gained through feeding, the numbers of larvae produced were fivefold less when compared to those raised at 30°C. At 20°C, 2.3 μg C was required to produce a single larva, whereas at 30°C it was 0.4 μg C. A rise in rearing temperature also influenced the molting rate positively. Observations on temporal variation in the gonad development of this species in a tropical coastal environment influenced by the monsoons indicated gonad development to be positively related to chlorophyll a concentration.  相似文献   

13.
A total of 12 feeding experiments were conducted in the northern Gulf of Aqaba during spring (March/April) and autumn (September/October) 2002 at the Marine Science Station (MSS) in Aqaba. Females of three species of clausocalanids were selected: Clausocalanus farrani, C. furcatus and Ctenocalanus vanus. Natural occurring particle (NOP) larger than 5 μm were investigated as food source. The ambient chlorophyll a concentration at sampling depth (∼70 m) ranged between 0.15 and 1.00 μg chl a l−1 and NOP concentrations ranged between 1.78 and 14.0 × 103 cells l−1 during the sampling periods. The division of particles into five size classes (5–10, 10–20, 20–50, 50–100 and >100 μm) revealed that most of the particles were found in the size classes below 50 μm (81–98%), while most of the natural occurring carbon (NOC) was concentrated in the size classes larger than 20 μm (70–95%). Ingestion rates were food density dependent rather than size dependent ranging between 0.02 and 1.65 × 103 NOP ind−1 day−1 and 0.01 and 0.41 μg NOC ind−1 day−1, respectively, equivalent to a body carbon (BC) uptake between 0.4 and 51.8% BC day−1. The share of the size classes to the total ingestion resembled in most cases the size class composition of the natural particle community.  相似文献   

14.
The storage of nitrate by phytoplankton cells during the early phases of upwelling was studied in coastal stations off northern Spain (southern Bay of Biscay) between 1990 and 1994. In this region, a persistent upwelling during summer is characterised by intermittent pulses of variable intensity, and increased nutrient concentrations in the surface layer. The main effect of an upwelling pulse on phytoplankton distribution is the shifting of the chlorophyll a and primary production maxima to near the surface. When the upwelling relaxes, thermal stratification of the water column occurs, and a distinct subsurface chlorophyll maximum develops below the production maximum. An accumulation of intracellular nitrate characterized the early phases of upwelling (mean = 2.73 μmol N m−3), maximum concentrations being attained at depths where biomass and production values were moderate. In contrast, phytoplankton cells from non-upwelling situations contained significantly lower concentrations of intracellular nitrate (mean = 0.17 μmol N m−3). The variations in the intracellular pool of nitrate may result from the differential allocation of resources within the cell as a result of variations in the energy available, since the uptake and assimilation of nitrate is a relatively expensive process involving several enzymatic systems. We hypothesize that nitrate storage by phytoplankton cells is characteristic of early phases of upwelling and is linked to patterns of carbon fixation. Average nitrogen budgets for upwelling and non-upwelling situations indicate that intracellular nitrate reserves are not responsible for maintaining high phytoplankton growth rates, since they only account for <2% of daily primary production during upwelling events. Received: 28 August 1996 / Accepted 3 December 1996  相似文献   

15.
 The physico-chemical microenvironment of larger benthic foraminifera was studied with microsensors for O2, CO2, pH, Ca2+ and scalar irradiance. Under saturating light conditions, the photosynthetic activity of the endosymbiotic algae increased the O2 up to 183% air saturation and a pH of up to 8.6 was measured at the foraminiferal shell surface. The photosynthetic CO2 fixation decreased the CO2 at the shell down to 4.7 μM. In the dark, the respiration of host and symbionts decreased the O2 level to 91% air saturation and the CO2 concentration reached up to 12 μM. pH was lowered relative to the ambient seawater pH of 8.2. The endosymbionts responded immediately to changing light conditions, resulting in dynamic changes of O2, CO2 and pH at the foraminiferal shell surface during experimentally imposed light–dark cycles. The dynamic concentration changes demonstrated for the first time a fast exchange of metabolic gases through the perforate, hyaline shell of Amphistegina lobifera. A diffusive boundary layer (DBL) limited the solute exchange between the foraminifera and the surrounding water. The DBL reached a thickness of 400–700 μm in stagnant water and was reduced to 100–300 μm under flow conditions. Gross photosynthesis rates were significantly higher under flow conditions (4.7 nmol O2 cm−3 s−1) than in stagnant water (1.6 nmol O2 cm −3 s−1), whereas net photosynthesis rates were unaffected by flow conditions. The Ca2+ microprofiles demonstrated a spatial variation in sites of calcium uptake over the foraminiferal shells. Ca2+ gradients at the shell surface showed total Ca2+ uptake rates of 0.6 to 4.2 nmol cm−2 h−1 in A. lobifera and 1.7 to 3.6 nmol cm−2 h−1 in Marginopora vertebralis. The scattering and reflection of the foraminiferal calcite shell increased the scalar irradiance at the surface up to 205% of the incident irradiance. Transmittance measurements across the calcite shell suggest that the symbionts are shielded from higher light levels, receiving approximately 30% of the incident light for photosynthesis. Received: 6 July 1999 / Accepted: 28 April 2000  相似文献   

16.
New-production (nitrate uptake) rates in the equatorial Pacific were estimated by parallel measurements of nitrate disappearance from sea water using a colorimetric method and of 15N-labelled nitrate (15NO3 ) incorporation into particulate organic nitrogen (PON) collected on GF/F filters (net nitrate uptake, conventional 15N-tracer method) and Anopore (0.2 μm) membranes. Regression analyses of 74 sample pairs gathered during 12 and 24 h productivity experiments revealed a significant positive relationship between decreasing nitrate level and 15NO3 accumulation into PON retained on GF/F filters, but the slopes of Model I and Model II regression lines were 1.18 and 1.29, respectively, suggesting that 15 to 22% of 15NO3 removed from the dissolved fraction were lost to another N-pool. Two possible avenues for the missing 15NO3 have been examined: uptake by submicron particles passed through the GF/F filters, and loss as dissolved organic nitrogen (DON). Nitrate uptake by small cells not recovered on GF/F filters, could be safely eliminated as a cause of loss, since 15NO3 uptake rates obtained from 15N entering PON collected on GF/F filters agreed well with those obtained from 15N entering PON collected on Anopore membranes (32 sample pairs). Inspection of the DON pool of 0.2 μm filtrates for excess-15N enrichment (20 samples) revealed that in nitrate-rich waters (equatorial upwelling between 1°N and 10°S), loss of 15NO3 as DO15N accounted for <5% of net nitrate uptake. In samples from subtropical oligotrophic waters (from 11°S southward), however, 15NO3 loss as DO15N represented up to 20% of net NO3 uptake. These results, as well as experimental considerations concerning the use of colorimetric and isotopic methods to measure new production show that: (1) earlier reported high discrepancies between nitrate decreases (ΔNO3 ) and 15NO3 incorporation into filterable particles (ΔNO3 /15NO3 incorporation >2) were probably erroneous; (2) the use of GF/F filters does not result in an underestimation of new production, although it was found to underestimate PON concentrations by up to 60%; (3) in the equatorial upwelling area (1°N to 10°S), which has high ambient nitrate levels (>2000 nmol l−1) but only slight changes in concentration (0 to 80 nmol l−1 d−1), new production is more accurately estimated by the isotopic method than by the chemical method; (4) in subtropical oligotrophic waters (from 11°S southward) with low ambient nitrate levels (0 to 100 nmol l−1), both procedures are appropriate as long as nitrate removal per incubation period is >3 nmol l−1 (lower rates are only detectable with the isotopic method); (5) the traditional 15N-tracer technique does not substantially underestimate net new-production in the equatorial Pacific, and failure to account for the loss of 15NO3 as DON, i.e. to estimate gross nitrate uptake (gross uptake = net uptake + 15N loss) tends to underestimate new production on an average by only 10%. Overall, the apparent low level of new production in the nitrate-rich area of the central equatorial Pacific seems to be a fact, and may be ascribable to other nutrient (macro and micro) deficiencies and/or to intense in situ recycling of ammonium and nitrate (regenerated production) rather than to inaccurate nitrate uptake rates measured with the classical 15N-tracer technique. Received: 24 November 1998 / Accepted 10 March 2000  相似文献   

17.
Morphology, elemental content and isotopic composition of leaves of the seagrasses Posidonia oceanica and Cymodocea nodosa were highly variable across the Illes Balears, a Spanish archipelago in the western Mediterranean, and varied seasonally at one site in the study area. The data presented in this paper generally expand the reported ranges of nitrogen, phosphorus, iron and arsenic content and δ13C and δ15N for these species. Nitrogen and phosphorus content of P. oceanica leaves also showed significant seasonal variability; on an annual basis, P. oceanica leaves averaged 1.55% N and 0.14% P at this monitoring site. Both N and P were more concentrated in the leaves in winter than in summer, with winter maxima of 1.76% N and 0.17% P and summer minima of 1.34% N and 0.11% P. There was no significant annual pattern observed in the δ13C of P. oceanica leaves, but there was a repeated 0.6‰ seasonal fluctuation in δ15N. Mean annual δ15N was 4.0‰; δ15N was lowest in May and it increased through the summer and autumn to a maximum in November. Over the geographic range of our study area, there were interspecific differences in the carbon, nitrogen and phosphorus content of the two species. Posidonia oceanica N:P ratios were distributed around the critical value of 30:1 while the ratios for C. nodosa were lower than this value, suggesting P. oceanica we collected was not consistently limited by N or P while C. nodosa tended toward nitrogen limitation. Nutrient content was significantly correlated to morphological indicators of plant vigor. Fe content of P. oceanica leaves varied by a factor of 5×, with a minimum of 31.1 μg g−1 and a maximum of 167.7 μg g−1. Arsenic was present in much lower tissue concentrations than Fe, but the As concentrations were more variable; the maximum concentration of 1.60 μg g−1 was eight times as high as the minimum of 0.20 μg g−1. There were interspecific differences in δ13C of the two species; C. nodosa was consistently more enriched (δ13C = −7.8 ± 1.7‰) than P. oceanica (−13.2 ± 1.2‰). The δ13C of both species decreased significantly with increasing water depth. Depth related and regional variability in the δ13C and δ15N of both species were marked, suggesting that caution needs to be exercised when applying stable isotopes in food web analyses.  相似文献   

18.
The water chemistry of 20 municipal water treatment plants in southern Sweden, representing various bedrock situations, and water qualities, were investigated. Four water samples, raw and treated, were collected from each plant and analyzed by predominantly ICP-OES and ICP-MS at four occasions from June to December, 2001. The concentrations of Ca, Mg, K, Na, HCO3 and a number of micronutrients, varied considerably in treated waters from the studied plants (ranges; Ca: 9.1–53.7 mg L−1, Mg: 1.4–10.9 mg L−1, K: 1.1–4.8 mg L−1, Na; 5.4–75.6 mg L−1, HCO3: 27–217 mg L−1). The elimination of Fe and Mn from raw water was efficient in all treatments investigated, giving concentrations in treated waters below the detection limits at some plants. Softening filters gave waters with Ca-concentrations comparable to the softest waters in this study. Adjustment of pH by use of chemicals like lye, soda or lime, modified the consumer water composition significantly, besides raising the pH. It was estimated that drinking water contributed to approximately 2.2–13% of the daily Ca uptake, if the gastrointestinal uptake efficiency from food and water was estimated to be around 50%. The corresponding figures for Mg was 1.0–7% and for F 0–59%. None of the studied elements showed any significant time trends in raw or treated waters during the follow-up period. The concentrations of potentially toxic metals such as Al, Pb and U were low and did not indicate risks for adverse health effects (ranges; Al: 0.5–2.3 μg L−1, Pb: 0–0.3 μg L−1, U: 0.2.5 μg L−1).  相似文献   

19.
An assessment of exposure to mercury in Changchun city has been undertaken. We estimated Hg exposure to members of the general population based on currently available information and our research. We also studied the Hg concentrations in scalp hair of adults. Adults have an estimated intake of all Hg species via all routes of 6.780 μg day−1 (excluding dental amalgam), which equates to an absorbed dose of 1.718 μg day−1. Fish consumption was the most important exposure route (12% of intake, 43% of absorbed dose). Furthermore, air, cereals and vegetables were important exposure routes, and these exposure were estimated for absorbed dosed at 0.296, 0.209 and 0.318 μg day−1, respectively. The mean Hg concentration in hair was 0.448 μg g−1 (range 0.092–10.463 μg g−1). Hg concentration in the hair of males was 0.422 μg g−1 (0.105–2.665 μg g−1), and was 0.474 μg g−1(0.092–10.463 μg g1) in the hair of females. Neither place of residence nor age had any significant effect on hair Hg concentrations.  相似文献   

20.
The time-course of uptake and elimination of benzo(a)pyrene (BaP) for the Pacific oyster, Crassostrea gigas and reproduction damage and reproductive outputs were studied. Sexually immature C. gigas broodstock were fed for 28 days with live algae grown in four BaP solutions of 0, 50, 500, and 5,000 μg L−1 (hereafter, control, 50, 500, and 5,000 oysters) and were subsequently conditioned to maturation by a feeding with BaP-free live algae under temperature manipulation for another 28 days. The 5,000 μg L−1 oysters gained a steady state concentration, around 30,000 ng g−1 d.w. for digestive gland, a week earlier compared to the 500 μg L−1 oysters. The earlier gain or longer persistence of the steady state concentration influenced elimination of BaP, with an eliminating trend for 500 μg L−1 oysters, while no elimination for 5,000 μg L−1 oysters. The maternal persistence of the steady state concentration resulted in significant damages in the reproductive success and their reproductive outputs in terms of the hatching rate and larval growth, survival, and settlement. The 50 μg L−1 oysters remained far below the steady state concentration, and showed a manifest eliminating behavior during the subsequent BaP-free 28 day maturation period. The reproductive success and initial larval events of 50 μg L−1 oysters were comparable to those of control. However, the damage potential of the 50 μg L−1 oysters might be more significant if their maternal exposure continued beyond 28 days, since the accumulation profile at this dose was linear.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号