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1.
以乙酸钠为碳源,分别考察了以石英砂和生物陶粒为滤料的2种反硝化滤池在不同C/N比条件下的脱氮效果,并对生物膜量和微生物种群进行了分析。结果表明:2种滤池对硝酸盐和TN都有较好的去除效果,且生物陶粒滤池要明显优于石英砂滤池;从经济性考虑,C/N比为4时的碳源投加量是最佳的,此时石英砂池和生物陶粒池出水TN平均浓度分别低于10 mg·L-1和5 mg·L-1,亚硝酸盐含量在0.5 mg·L-1以下。2柱的生物膜量沿着水流方向都逐渐减少,且生物陶粒池的生物膜量明显高于石英砂池。高通量测序结果表明,2滤池系统的物种多样性相差不大,在门的分类水平上,2个系统的主要优势菌群都属于Proteobacteria(变形菌门),含量在94%以上;在属的分类水平上,2柱的反硝化功能菌都主要是Dechloromonas(脱氯单胞菌属)和Thauera(陶厄氏菌属),但含量上存在一定差异。 相似文献
2.
Background, aim, and scope Polysaccharides are renewable resources representing an important class of polymeric materials of biotechnological interest,
offering a wide variety of potentially useful products to mankind. Exopolysaccharides (EPSs) of microbial origin with a novel
functionality, reproducible physico-chemical properties, stable cost and supply, became a better alternative to polysaccharides
of algal origin. EPSs are believed to protect bacterial cells from desiccation, heavy metals or other environmental stresses,
including hostimmune responses, and to produce biofilms, thus enhancing the cells chances of colonising special ecological
niches. One of the most important stress factor is salt stress for microorganisms. The present investigation is aimed to determine
correlation between salt resistance and EPS production by three cyanobacterial isolates ( Synechocystis sp. BASO444, Synechocystis sp. BASO507 and Synechocystis sp. BASO511). It is also aimed to investigate the effect of salt concentrations on EPS production by cyanobacteria and effect
of salt on monosaccharide composition of EPS. 相似文献
3.
ABSTRACT The use of activated carbon fiber (ACF) filters for the capture of particulate matter and elemental Hg is demonstrated. The pressure drop and particle collection efficiency characteristics of the ACF filters were established at two different face velocities and for two different aerosols: spherical NaCl and combustion-generated silica particles. The clean ACF filter specific resistance was 153 kg m -2 sec -1. The experimental specific resistance for cake filtration was 1.6 × 10 6 sec -1 and 2.4 × 10 5 sec -1 for 0.5- and 1.5-μm mass median diameter particles, respectively. The resistance factor R was approximately 2, similar to that for the high-efficiency particulate air filters. There was a discrepancy in the measured particle collection efficiencies and those predicted by theory. The use of the ACF filter for elemental Hg capture was illustrated, and the breakthrough characteristic was established. The capacity of the ACF filter for Hg capture was similar to other powdered activated carbons. 相似文献
4.
活性污泥中的胞外聚合物EPS含黏性蛋白类物质并高度亲水,调控污泥中溶解性EPS和溶解性蛋白类物质是改善污泥脱水的有效途径。通过使用不同炭材料调理污泥的方法来观察污泥脱水性能的变化,深入解析污泥絮体及EPS含量和组分的变化特征。结果表明:炭材料调理污泥可以改善其脱水性,且炭材料吸附容量越大对污泥脱水性改善效果越好;不同炭材料对污泥絮体影响效果不同,经过未改性活性炭(AC-0)、HNO3改性活性炭(AC-1)和石墨调理的污泥絮体粒径变大,经酸碱交替改性活性炭(AC-5)调理的污泥絮体粒径变小;炭材料调理后EPS中蛋白类的荧光峰A、B和腐殖酸、富里酸的荧光峰C、D都得到有效降低,且其对腐殖酸和富里酸的吸附效果比蛋白类物质要好,对低分子质量物质和中分子质量物质的吸附效果要好于对大分子质量物质的吸附效果。 相似文献
5.
Permeable reactive barriers (PRBs) are an alternative technology to treat mine drainage containing sulfate and heavy metals. Two column experiments were conducted to assess the suitability of an organic carbon (OC) based reactive mixture and an Fe 0-bearing organic carbon (FeOC) based reactive mixture, under controlled groundwater flow conditions. The organic carbon mixture contains about 30% (volume) organic carbon (composted leaf mulch) and 70% (volume) sand and gravel. The Fe 0-bearing organic carbon mixture contains 10% (volume) zero-valent iron, 20% (volume) organic carbon, 10% (volume) limestone, and 60% (volume) sand and gravel. Simulated groundwater containing 380 ppm sulfate, 5 ppm As, and 0.5 ppm Sb was passed through the columns at flow rates of 64 (the OC column) and 62 (the FeOC column) ml d − 1, which are equivalent to 0.79 (the OC column) and 0.78 (the FeOC column) pore volumes (PVs) per week or 0.046 m d − 1 for both columns. The OC column showed an initial sulfate reduction rate of 0.4 µmol g (OC) − 1 d − 1 and exhausted its capacity to promote sulfate reduction after 30 PVs, or 9 months of flow. The FeOC column sustained a relatively constant sulfate reduction rate of 0.9 µmol g (OC) − 1 d − 1 for at least 65 PVs (17 months). In the FeOC column, the δ34S values increase with the decreasing sulfate concentration. The δ34S fractionation follows a Rayleigh fractionation model with an enrichment factor of 21.6‰. The performance decline of the OC column was caused by the depletion of substrate or electron donor. The cathodic production of H 2 by anaerobic corrosion of Fe probably sustained a higher level of SRB activity in the FeOC column. These results suggest that zero-valent iron can be used to provide an electron donor in sulfate reducing PRBs. A sharp increase in the δ13C value of the dissolved inorganic carbon and a decrease in the concentration of HCO 3− indicate that hydrogenotrophic methanogenesis is occurring in the first 15 cm of the FeOC column. 相似文献
6.
Samples of humic substances were obtained from a waterworks at Fuhrberg, Germany. The material had a bimodal molecular size distribution with 40% of the total carbon in the 50,000–100,000-D (nominal molecular weight, NMW, in daltons) size fraction and 50% of the carbon in the <10,000-D (NMW) size fraction. The fulvic and humic acids isolated from the bulk humic substances were low in nitrogen content and had low H/C atomic ratios. Furthermore, the fulvic and humic acids had very similar elemental, spectral and copper binding characteristics. Over 70% of the carbon in both the fulvic and humic acids was present in aromatic or aliphatic groups, with 13C NMR analyses indicating approximately even distribution among the two types. Competitive elemental binding studies indicated that Ca 2+, Mg 2+, Al 3+ and Fe 3+ do not effectively compete for copper binding sites on these compounds. In humic acids, these cations are predominantly bond by carboxylic groups. 相似文献
7.
It is necessary to understand the bioflocculation, settling and dewatering characteristics in the activated sludge process in order to establish more efficient operational strategies. The influences of carbon source on the extracellular polymeric substances (EPS) and flocculation, settling and dewatering properties of the activated sludge were investigated. Laboratory-scale completely mixed activated sludge processes were used to grow the activated sludge with different carbon sources of starch, glucose and sodium acetate. The sludge fed with acetate had highest loosely bound EPS (LB-EPS) and that fed with starch lowest. The amount of tightly bound EPS (TB-EPS), protein content in LB-EPS, polysaccharide content and protein contents in TB-EPS, were independent of the influent carbon source. The polysaccharide content in LB-EPS of the activated sludge fed with sodium acetate was lower slightly than those of starch and glucose. The sludge also had a nearly consistent flocs size and the sludge volume index (SVI) value. ESS content of the sludge fed with sodium acetate was higher initially, although it was similar to those fed with glucose and starch finally. However, the specific resistance to filtration and normalized capillary suction time fluctuated first, but finally were stable at around 5.0 × 10 8 m kg −1 and 3.5 s L g −1 SS, respectively. Only the protein content in LB-EPS weakly correlated with the flocs size and SVI of the activated sludge. But there was no correlation between any other EPS contents or components and the physicochemical properties of the activated sludge. 相似文献
8.
There has been a recent increase in the use of silver nanoparticles (Ag NPs) in a wide range of consumer products due to their highly effective antimicrobial properties. However, Ag NPs give cause for concern since their wide use makes them likely to be released into aquatic ecosystems and potentially affect natural bacterial communities. In this study marine biofilms were grown in situ in a coastal site (Singapore Harbour) and exposed in the laboratory for a further 24 h to 0-2000 μg L −1 of well characterised Ag NPs. Increasing concentrations of Ag NPs caused a significant decrease in biofilm volume and biomass, and Ag uptake by biofilms per unit of volume was also dependent on concentration. Terminal fragment length polymorphisms and subsequent cluster and phylogenetic analysis showed the presence of major bacterial groups in biofilms irrespective of treatment with Ag NPs. This implies that even at the highest concentrations studied these taxonomic groups were not displaced. Nevertheless, biofilm succession was impeded on Ag NP treated biofilms, affecting the relative abundance of major bacterial groups in the biofilm community, with potential longer term effects on biofilm development and function. 相似文献
9.
以嘉兴市某水厂沉后水为进水进行实验,研究炭砂滤池对污染物的去除性能,以及炭砂滤池取代活性炭+砂滤池的可能性,探讨了短流程工艺的适用性。结果表明,炭砂滤池能有效去除水中的浊度、氨氮、CODMn和铁、锰等污染物,与活性炭柱相比,炭砂滤池在降低出水浊度和水中颗粒数方面具有一定优势,即砂垫层对控制出水浊度和保障微生物安全能够起到积极作用。在实验条件下,增加活性炭层厚度或者降低滤速,在一定程度上有利于提高对CODMn的去除率。在设计滤速为9~10 m/h,CODMn〈4.5 mg/L时,可直接以炭砂滤池取代活性炭池+砂滤池,仍然能保证出水CODMn〈3 mg/L。 相似文献
10.
It is quite rare to find biodegradation in rapid sand filtration for drinking water treatment. This might be due to frequent backwashes and low substrate levels. High chlorine concentrations may inhibit biofilm development, especially for plants with pre-chlorination. However, in tropical or subtropical regions, bioactivity on the sand surface may be quite significant due to high biofilm development—a result of year-round high temperature. The objective of this study is to explore the correlation between biodegradation and chlorine concentration in rapid sand filters, especially for the water treatment plants that practise pre-chlorination. In this study, haloacetic acid (HAA) biodegradation was found in conventional rapid sand filters practising pre-chlorination. Laboratory column studies and field investigations were conducted to explore the association between the biodegradation of HAAs and chlorine concentrations. The results showed that chlorine residual was an important factor that alters bioactivity development. A model based on filter influent and effluent chlorine was developed for determining threshold chlorine for biodegradation. From the model, a temperature independent chlorine concentration threshold (Cl threshold) for biodegradation was estimated at 0.46-0.5 mg L −1. The results imply that conventional filters with adequate control could be conducive to bioactivity, resulting in lower HAA concentrations. Optimizing biodegradable disinfection by-product removal in conventional rapid sand filter could be achieved with minor variation and a lower-than-Cl threshold influent chlorine concentration. Bacteria isolation was also carried out, successfully identifying several HAA degraders. These degraders are very commonly seen in drinking water systems and can be speculated as the main contributor of HAA loss. 相似文献
11.
This study discovered that biofilm extracellular polymeric substances (EPS) are biodegradable by their own producers and by other microorganisms when they are starved. The study was performed in a comparative fashion to examine the biodegradability of biofilm EPS by the microorganisms from the original biofilm (its own producers) and from activated sludge (other microorganisms). Four distinctive phases were observed during EPS biodegradation. In the first phase, instantaneous concentration increases of carbohydrate and protein in the test solutions were observed when EPS was added; in the second phase, easily biodegradable EPS from the added EPS was quickly utilized; in the third phase, microorganisms began to produce soluble EPS, using the minimally biodegradable EPS left from the previously added EPS; in the fourth phase, cells consumed the newly produced EPS and microbial activity gradually stopped. This study suggests that EPS can be used as a substrate, and that the EPS carbohydrate can be utilized faster than the EPS protein. The EPS utilization rates (including carbohydrate and protein) in the activated sludge suspension were greater than those in the biofilm suspension. It may take microorganisms longer to get acclimated to a new nutrient environment if they are in a starved state. 相似文献
12.
Extracellular polymeric substances (EPS) are an extracellular matrix found in sludge which plays a crucial role in flocculation by interacting with the organic solids. Therefore, to enhance pretreatment of sludge, EPS have to be removed. In this study, EPS were removed with a chemical extractant, NaOH, to enhance the bacterial pretreatment. A lysozyme secreting bacterial consortium was isolated from the waste activated sludge (WAS). The result of density gradient gel electrophoresis (DGGE) analysis revealed that the isolated consortium consists of two strains. The two novel strains isolated were named as Jerish03 (NCBI accession number KC597266) and Jerish 04 (NCBI accession number KC597267) and they belong to the genus Bacillus. Pretreatment with these novel strains enhances the efficiency of the aerobic digestion of sludge. Sludge treated with the lysozyme secreting bacterial consortium produced 29 % and 28.5 % increase in suspended solids (SS) reduction and chemical oxygen demand (COD) removal compared to the raw activated sludge (without pretreatment) during aerobic digestion. It is specified that these two novel strains had a high potential to enhance WAS degradation efficiency in aerobic digestion. 相似文献
13.
To develop a bacterial bioaugmentation system for fluorine-containing industrial wastewater treatment, optimal conditions for 4-fluoroaniline (4-FA) degradation and autoinducer release in Acinetobacter sp. TW were determined. Quorum sensing in biofilms of strain TW was also investigated. Different optimal conditions exist for 4-FA degradation and autoinducer release, particularly with regard to pH. Quorum sensing modulates extracellular polymeric substance (EPS) secretion and biofilm formation in the strain but plays no role in 4-FA degradation. Under optimal conditions for 4-FA degradation, the release of N-3-oxo-hexanoyl-homoserine lactone (3-oxo-C 6-HSL) and N-hexanoyl-homoserine lactone (C 6-HSL) in strain TW was significantly lower than required for quorum sensing. Under optimal conditions for autoinducer release, on the other hand, 3-oxo-C 6-HSL and C 6-HSL levels exceeded the quorum sensing thresholds, thereby inducing EPS secretion and biofilm formation. We conclude that the optimal conditions for autoinducer release (25 °C, pH 5, 800 mg L ?1 4-FA, and 0 % NaCl) are suitable for bacterial colonization in bioaugmentation, while those for 4-FA degradation (25–30 °C, pH 8 and 800 mg L ?1 4-FA) maximize the system performance after colonization. 相似文献
14.
Twenty-one samples were collected during the dry season (26 January–28 February 2004) at 12 sites in and around Addis Ababa, Ethiopia and analyzed for particulate matter with aerodynamic diameter <10 μm (PM 10) mass and composition. Teflon-membrane filters were analyzed for PM 10 mass and concentrations of 40 elements. Quartz-fiber filters were analyzed for chloride, sulfate, nitrate, and ammonium ions as well as elemental carbon (EC) and organic carbon (OC) content. Measured 24-h PM 10 mass concentrations were <100 and 40 μg m −3 at urban and suburban sites, respectively. PM 10 lead concentrations were <0.1 μg m −3 for all samples collected, an important finding because the government of Ethiopia had stopped the distribution of leaded gasoline a few months prior to this study. Mass concentrations reconstructed from chemical composition indicated that 34–66% of the PM 10 mass was due to geologically derived material, probably owing to the widespread presence of unpaved roads and road shoulders. At urban sites, EC and OC compounds contributed between 31% and 60% of the measured PM 10 while at suburban sites carbon compounds contributed between 24% and 26%. Secondary sulfate aerosols were responsible for <10% of the reconstructed mass in urban areas but as much as 15% in suburban sites, where PM 10 mass concentrations were lower. Non-volatile particulate nitrate, a lower limit for atmospheric nitrate, constituted <5% and 7% of PM 10 at the urban and suburban sites, respectively. At seven of the 12 sites, real-time PM 10 mass, real-time carbon monoxide (CO), and instantaneous ozone (O 3) concentrations were measured with portable nephelometers, electrochemical analyzers, and indicator test sticks, respectively. Both PM 10 and CO concentrations exhibited daily maxima around 7:00 and secondary peaks in the late afternoon and evening, suggesting that those pollutants were emitted during periods associated with motor-vehicle traffic, food preparation, and heating of homes. The morning concentration maxima were likely accentuated by stable atmospheric conditions associated with overnight surface temperature inversions. Ozone concentrations were measured near mid-day on filter sample collection days and were in all cases <45 parts per billion. 相似文献
15.
Up-flow biological activated carbon (UBAC) filter has been widely used in waterworks due to its less hydraulic loss, stronger biodegradation ability, and the prevention of excessive biomass growth relative to down-flow BAC treatment. In this study, the different removal efficiency (DRE) of disinfection byproduct precursors between dichloroacetonitrile (DCAN) and dichloroacetamide (DCAcAm) was evaluated when UBAC filter was used as advanced treatment process. Results showed that the UBAC filter with approximately 36 months of usage time had a poor performance in the removal of DCAcAm formation potential (FP) (i.e. 9.3–19.1%) compared to DCAN FP (i.e., 22.5–34.1%). After chlorination of UBAC effluent, the hydrolysis of DCAN to form DCAcAm only partly contributed to the DRE variations of both DCAN FP and DCAcAm FP. Using the high-throughput sequencing technology and the redundancy analysis (RDA), the second dominant genus Bacillus in UBAC filter, which may transform precursors of DCAN into inorganic matters, could be another reason that led to the DRE in DCAN and DCAcAm FP. The formation and leakage of soluble microbial products (SMPs) was identified by excitation-emission matrix (EEM) peak intensities as well as variation of biological index (BIX). The SMPs released into UBAC effluent, favoring the formation of DCAcAm, also contributed to the precursors of both DCAN and DCAcAm, causing a poor removal performance in DCAcAm FP by UBAC filter. 相似文献
16.
实验通过对传统臭氧-生物活性炭工艺的调整,采用臭氧-曝气生物活性炭滤池-砂滤池工艺,对比研究了4mm柱状炭、4 mm破碎炭和1.5 mm柱状炭3种不同类型活性炭用于给水深度处理的运行效果。结果表明,粒径为1.5 mm的柱状炭对有机物和氨氮的去除效果以及出水浊度方面优于其他2种活性炭,但炭滤池水头损失偏大,且不够稳定,适当增加柱状炭的粒径可稳定和降低滤池的水头损失。 相似文献
17.
This study performed a workplace evaluation of emission control using available air sampling filters and characterized the emitted particles captured in filters. Characterized particles were contained in the exhaust gas released from carbon nanotube (CNT) synthesis using chemical vapor deposition (CVD). Emitted nanoparticles were collected on grids to be analyzed using transmission electron microscopy (TEM). CNT clusters in the exhaust gas were collected on filters for investigation. Three types of filters, including Nalgene surfactant-free cellulose acetate (SFCA), Pall A/E glass fiber, and Whatman QMA quartz filters, were evaluated as emission control measures, and particles deposited in the filters were characterized using scanning transmission electron microscopy (STEM) to further understand the nature of particles emitted from this CNT production. STEM analysis for collected particles on filters found that particles deposited on filter fibers had a similar morphology on all three filters, that is, hydrophobic agglomerates forming circular beaded clusters on hydrophilic filter fibers on the collecting side of the filter. CNT agglomerates were found trapped underneath the filter surface. The particle agglomerates consisted mostly of elemental carbon regardless of the shapes. Most particles were trapped in filters and no particles were found in the exhaust downstream from A/E and quartz filters, while a few nanometer-sized and submicrometer-sized individual particles and filament agglomerates were found downstream from the SFCA filter. The number concentration of particles with diameters from 5 nm to 20 µm was measured while collecting particles on grids at the exhaust piping. Total number concentration was reduced from an average of 88,500 to 700 particle/cm 3 for the lowest found for all filters used. Overall, the quartz filter showed the most consistent and highest particle reduction control, and exhaust particles containing nanotubes were successfully collected and trapped inside this filter.Implications: As concern for the toxicity of engineered nanoparticles grows, there is a need to characterize emission from carbon nanotube synthesis processes and to investigate methods to prevent their environmental release. At this time, the particles emitted from synthesis were not well characterized when collected on filters, and limited information was available about filter performance to such emission. This field study used readily available sampling filters to collect nanoparticles from the exhaust gas of a carbon nanotube furnace. New agglomerates were found on filters from such emitted particles, and the performance of using the filters studied was encouraging in terms of capturing emissions from carbon nanotube synthesis. 相似文献
18.
A system of recirculating channels was used in this study to examine the long-term effects (29 d) of environmentally realistic concentrations of the herbicide diuron (from 0.07 to 7 μg L −1) on biofilm communities. The autotrophic activity of biofilms was affected by this herbicide, as reflected by a marked decrease in the photosynthetic efficiency. Diuron exposure also increased chlorophyll- a content and reduced the biovolume of diatom taxa at low concentrations. The effects on bacteria were also remarkable. Bacterial abundance was reduced after a week of exposure to the herbicide at a range of concentrations. Effects were on the number of live bacteria and on the increase in the leucine-aminopeptidase activity. It is suggested that inputs of herbicides to the river ecosystem at low concentrations may cause a chain of effects in the biofilm, which include inhibitory effects on algae but also indirect effects on the relationships between biofilm components. 相似文献
19.
利用活性污泥-生物膜一体化反应器处理含苯酚废水,考察了反应器对苯酚和COD的处理效果以及反应器运行中生物膜干质量(SS)、挥发性干质量(VSS)、活性生物量、脱氢酶活性(DHA)、胞外聚合物(EPS)的变化,探究了生物膜特性与废水处理效果之间的关系。结果表明:在进水苯酚质量浓度由50 mg·L −1逐步提高到500 mg·L −1的过程中,苯酚和COD去除率均呈先降后升的趋势;当进水苯酚质量浓度为250 mg·L −1时,反应器能适应苯酚冲击,苯酚和COD去除率分别稳定在97%和60%以上;当进水苯酚质量浓度为500 mg·L −1时,苯酚去除率可达到99%,同期SS、VSS、活性生物量及DHA(22.03~57.07 mg·g −1)的变化亦反映出生物膜性能的提升,说明反应器对苯酚质量浓度变化的适应能力较强。此外,EPS质量分数为42.99~310.51 mg·g −1,蛋白质(PN)与多糖(PS)的质量比为0.67~1.39,且当初始苯酚质量浓度为250 mg·L −1时,PN/PS值最高,EPS亲水性低,生物膜可高效降解苯酚。以上研究结果表明,逐渐提高进水苯酚质量浓度能有效提高活性污泥-生物膜一体化反应器对苯酚的适应性和降解率。 相似文献
20.
Extracellular polymeric substances (EPS) are, along with microbial cells, the main components of the biological sludges used in wastewater treatment and natural biofilms. EPS play a major role in removing pollutants from water by means of sorption. The ability of soluble EPS (S-EPS) and bound EPS (B-EPS) derived from various bacterial aggregates (flocs, granules, biofilms) to bind at pH 7.0?±?0.1 to two pharmaceutical substances, acetaminophen (ACE) and erythromycin ethylsuccinate (ERY), has been investigated using the fluorescence quenching method. Two intense fluorescence peaks, A (Ex/Em range, 200–250/275–380 nm) and B (Ex/Em range, 260–320/275–360 nm), corresponding respectively to the aromatic protein region and soluble microbial by-product-like region, were identified in a three-dimensional excitation-emission matrix of EPS samples. The fluorescence peak, which corresponds to humic-like substances, was also identified though at low intensity. The ability of EPS to bind ACE was found to exceed that for ERY. The aromatic protein fraction of EPS displays a slightly higher affinity for drugs than that shown by the soluble microbial by-product-like fraction. The S-EPS and B-EPS present the same affinity for ACE and ERY. The effective quenching constants (log K) derived from the Stern–Volmer Equation equaled at peak A (with S-EPS): 3.7?±?0.2 to 4.0?±?0.1 for ACE and 2.1?±?0.3 to 2.7?±?0.1 for ERY. With B-EPS, these values were 3.9?±?0.1 to 4.0?±?0.1 for ACE and 2.0?±?0.2 to 2.6?±?0.1 for ERY. Our results suggest that the weaker EPS affinity for ERY than for ACE serves to partially explain why only about 50–80 % of ERY is removed from wastewater at the treatment plant. Moreover, this work demonstrates that EPS from natural river biofilms are able to bind drugs, which in turn may limit the mobility of drugs in natural waters. 相似文献
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