洞庭湖区湿地生态系统存在的问题及其对策研究

洞庭湖是我国最大的淡水湖泊湿地景观生态系统之一,湿地资源丰富.由于泥沙淤积,洞庭湖区调蓄容积减少、洪水位不断抬升、江湖关系改变.在加重湖区的防洪负担、造成严重的洪涝灾害的同时,也降低了湖泊水体对各种污染物的稀释自净能力和水环境承载能力,导致湖泊湿地生态系统严重退化.本文分析了洞庭湖区湿地生态系统的功能,找出了导致洞庭湖区湿地生态系统退化的原因,并针对原因提出了一系列对策,以期恢复其原有功能.     

哈尔滨市松江湿地保护现状及生态修复策略研究

松江湿地是中国面积最大的河流型城市湿地.然而,随着城市化进程加快,生态功能正在逐步退化.目前,从实地调研情况看,松花江哈尔滨段下游湿地保护较好;而上游湿地保护工作不到位,大量湿地丧失,生态系统退化严重;整体看来,松江湿地生态系统受人为干扰严重,湿地片段化、斑块化现象严重.针对这一现状,列出了湿地目前存在的问题并分析退化和受损的原因,同时,筛选出了适合松江湿地的修复技术及生态恢复方案.     

我国湿地补水生态补偿机制探析

近年来,由于全球气候变化及社会经济发展的双重影响,我国许多重要湿地面临着严重的缺水威胁.许多湿地的生态功能严重退化或丧失,湿地生态系统稳定性受到严重破坏,一部分国际重要湿地正面临被列入《湿地公约》“黑名单”的危险,湿地生态补水迫在眉睫.2011年中央政府工作报告中提出要“继续实施重大生态修复工程,加强重点生态功能区保护和管理……加强湿地保护与恢复”.     

退化湿地生态系统的生态恢复与管理——以美国Hackensack湿地保护区为例

受城市化、工业化和地球温室效应等人类活动与自然因素的影响,美国Hackensack湿地保护区正面临着面积不断减少、景观破碎化、外来物种入侵、河水污染增加、盐碱化程度增加和范围扩大等生态胁迫。稳定湿地面积,重建湿地的复合景观,丰富生物多样性,恢复潮水的正常规律,减少水体污染,控制外来种的入侵与扩张,促进湿地原有植物的定居与扩增及原有植被的恢复是Hackensack湿地恢复的关键,也是恢复工程设计与实施的核心。退化湿地生态恢复的管理则以植被的生长、芦苇的扩散方式及动态、鱼类和蟹类的种群动态、鸟类的种类及其对生境地的利用情况对生态系统功能恢复状况进行生物检验,有效监控退化湿地生态系统的恢复动态,研究其恢复规律,进而采取相应的管理措施,科学调控生态恢复进程。     

加强湿地保护改善生态环境

湿地生态系统是地球生态系统的大规模重要组成部分,是一个丰富的自然生物多样性中主要的居住环境和人文景观、水质、水净化、防洪,同时保持生态系统,如气候变化,不能被其他生态功能取代。本文介绍了湿地保护对策和湿地生态恢复技术,并提出为了实现人与自然而和谐均衡发展,保持生态系统的平衡,改善生态状况的湿地主要研究技术提供参考。     

鄱阳湖湿地生态系统退化的社会经济根源研究

鄱阳湖湿地生态系统是一个复杂、开放的交互系统。近些年,鄱阳湖湿地生态系统退化有加剧的趋势,其中有自然的原因,但更重要的是在社会经济发展过程中引起自然环境的破坏和生态规律的波动。人口持续增长、不合理的经济发展模式、湿地保护相关政策的缺失等都加剧了湿地生态系统的退化。加强湿地管理与保护、调整区域经济发展方式、构建环鄱阳湖生态经济区等措施,对保护和开发鄱阳湖湿地有重要的作用。     

东部平原地区湖泊湿地的恢复与可持续发展对策

湖泊湿地是我国的重要湿地生态系统,具有许多经济价值和社会服务功能,合理开发和利用湖泊湿地资源有利于促进我国广大湖区的可持续发展。结合有关东部平原地区湖泊湿地的研究,提出该区湖泊湿地的恢复与可持续发展对策,指出湖泊湿地的恢复应坚持生态、经济和社会可持续发展的原则,并根据具体的生态环境状况来进行,旨在为恢复与保护湖泊湿地的实际操作提供科学依据。     

新疆艾比湖周边居民湿地生态系统健康保护意识调查分析

采用问卷调查的方法,试图真实反映艾比湖周边居民对艾比湖湿地生态系统退化的认知及对艾比湖湿地生态系统健康保护意识的强弱.结果表明：（1）艾比湖周边居民对湿地生态系统退化带来的危害有较为清晰地认识,99％被调查者意识到了艾比湖湿地生态系统退化对周边环境会带来不同程度的危害,78.2％调查对象认为艾比湖湿地生态系统的退化主要是由人类活动引发的,通过正规渠道获取艾比湖湿地生态系统退化信息不够通畅;（2）调查对象对艾比湖湿地生态系统退化情况持有较高的关注度,但支持度不高,85.6％的被调查者对退化湿地的生态恢复有支付意愿;（3）65.5％调查对象对艾比湖湿地生态恢复有信心,但对当地政府宣传和保护艾比湖湿地的力度满意度不高,因此政府部门还需进一步加强对艾比湖的宣传和保护力度.     

盘锦市湿地资源利用与恢复生态功能探讨

分析了盘锦市湿地由于不合理的开发利用,导致生态功能受损状况;为防止湿地资源的过度开发,保护湿地生态系统的结构、物种、功能效应,使湿地资源永续利用,提出了具有可操作性的恢复措施。     

把好湿地保护开发“平衡秤”

正今年是我国加入《湿地公约》22周年,湿地作为重要的自然资源和生态系统,具有不可替代的生态效益、经济效益和社会效益。然而,我国对湿地的保护与管理,在重视程度上,还不足以与当前建设"生态文明"的迫切要求相匹配,湿地面积减少、功能退化的趋势尚未得到根本遏制,湿地仍是最脆弱、最容易遭到侵占和破坏的生态系统。进一步重视和加强对我国有限湿地资源的保护,已是当务之急。要不断完善湿地保护规划,强化立法与考核,改变目前我国湿地保护零碎、分散的局面,在制度上规范湿地管理行为;理顺各方利益关系,平衡湿地保护与开发,妥善处理好     

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis （DGGE） and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity （both in gene richness and diversity index values） and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor （50 mg/kg） recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos （150 mg/kg） caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos （250 mg/kg） produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes （bands） responded differently to the impact of agrochemicals： four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the influence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affiliated with the alpha- and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.     

Arsenic uptake by arbuscular mycorrhizal maize （Zea mays L.） grown in an arsenic-contaminated soil with added phosphorus

The effects of arbuscular mycorrhizal (AM) fungus (Glomus mosseae) and phosphorus (P) addition (100 mg/kg soil) on arsenic (As) uptake by maize plants (Zea mays L.) from an As-contaminated soil were examined in a glasshouse experiment.Non-mycorrhizal and zero-P addition controls were included.Plant biomass and concentrations and uptake of As,P,and other nutrients,AM colonization,root lengths,and hyphal length densities were determined.The results indicated that addition of P significantly inhibited root colonization and development of extraradical mycelium.Root length and dry weight both increased markedly with mycorrhizal colonization under the zero-P treatments,but shoot and root biomass of AM plants was depressed by P application.AM fungal inoculation decreased shoot As concentrations when no P was added,and shoot and root As concentrations of AM plants increased 2.6 and 1.4 times with P addition,respectively.Shoot and root uptake of P,Mn,Cu,and Zn increased,but shoot Fe uptake decreased by 44.6%,with inoculation, when P was added.P addition reduced shoot P,Fe,Mn,Cu,and Zn uptake of AM plants,but increased root Fe and Mn uptake of the nonmycorrhizal ones.AM colonization therefore appeared to enhance plant tolerance to As in low P soil,and have some potential for the phytostabilization of As-contaminated soil,however,P application may introduce additional environmental risk by increasing soil As mobility.     

Degradation of metabolites accumulated of benzo[a]pyrene by coupling Penicillium Chrysogenum with KMnO4

Several main metabolites of benzo[a]pyrene （BaP） formed by Penicillium chrysogenum, Benzo[a]pyrene-1,6-quinone （BP 1,6- quinone）, trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene （BP 7,8-diol）, 3-hydroxybenzo[a]pyrene （3-OHBP）, were identified by high-performance liquid chromatography （HPLC）. The three metabolites were liable to be accumulated and were hardly further metabolized because of their toxicity to microorganisms. However, their further degradation was essential for the complete degradation of BaP. To enhance their degradation, two methods, degradation by coupling Penicillium chrysogenum with KMnO4 and degradation only by Penicillium chrysogenum, were compared; Meanwhile, the parameters of degradation in the superior method were optimized. The results showed that （1） the method of coupling Penicillium chrysogenum with KMnO4 was better and was the first method to be used in the degradation of BaP and its metabolites; （2） the metabolite, BP 1,6-quinone was the most liable to be accumulated in pure cultures; （3） the effect of degradation was the best when the concentration of KMnO4 in the cultures was 0.01% （w/v）, concentration of the three compounds was 5 mg/L and pH was 6.2. Based on the experimental results, a novel concept with regard to the bioremediation of BaP-contaminated environment was discussed, considering the influence on environmental toxicity of the accumulated metabolites.     

Impact of atrazine and nitrogen fertilizers on the sorption of chlorotoluron in soil and model sorbents

Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior of chlorotoluron. At the same concentration of N, sorption of chlorotoluron decreased as the concentration of atrazine increased on the day 0 and 6 in soil, respectively. The sorption of chlorotoluron increased from 0 to 6 d when soils were preincubated with deionized water, ammonium sulfate and urea solution for 6 d. That indicated incubation time was one of the most important factors for the sorption of chlorotoluron in nitrogen fertilizers treatments. The individual sorption isotherms of chlorotoluron in rubbery polymer and silica were strictly linear in single solute system, but there were competition sorption between pesticides or between pesticides and nitrogen fertilizers. That indicated the sorption taken place by concurrent solid-phase dissolution mechanism and sorption on the interface of water-organic matter or water-mineral matter.     

Potential of plant polyphenol oxidases in the decolorization and removal of textile and non-textile dyes

In this study an effort has been made to use plant polyphenol oxidases; potato （Solanum tuberosum） and brinjal （Solanum melongena）, for the treatment of various important dyes used in textile and other industries. The ammonium sulphate fractionated enzyme preparations were used to treat a number of dyes under various experimental conditions. Majority of the treated dyes were maximally decolorized at pH 3.0. Some of the dyes were quickly decolorized whereas others were marginally decolorized. The initial first hour was sufficient for the maximum decolorization of dyes. The rate of decolorization was quite slow on long treatment of dyes. Enhancement in the dye decolorization was noticed on increasing the concentration of enzymes. The complex mixtures of dyes were treated with both preparations of polyphenol oxidases in the buffers of varying pH values. Potato polyphenol oxidase was significantly more effective in decolorizing the dyes to higher extent as compared to the enzyme obtained from brinjal polyphenol oxidase. Decolorization of dyes and their mixtures, followed by the formation of an insoluble precipitate, which could be easily removed simply by centrifugation.     

Effects of two sludge application on fractionation and phytotoxicity of zinc and copper in the soil

The potential harm of heavy metals is a primary concern in application of sludge to the agricultural land. A pot experiment was conducted to evaluate the effect of two sludges on fractionation of Zn and Cu in soil and their phytotoxicity to pakchoi. The loamy soil was mixed with 0%, 20%, 40%, 60% and 80% (by weight) of digested sewage sludge (SS) and composted sludge (SC). The additions of both sludges caused a significant raise in all fractions, resulting in that exchangeable (EXCH) and organic bound (OM) became predominance of Zn and organic bound Cu occupied the largest portion. There was more available amount of Zn and Cu in SS treatments than SC treatments. During the pot experiment, the concentration of Zn in EXCH, carbonate (CAR) and OM and Cu in EXCH and OM fractions decreased in all treatments, so their bioavailability reduced. Germination rate and plant biomass decreased when the addition rate was high and the best yield appeared in 20% mixtures at the harvest of pakchoi. The two sludges increased tissue contents of Zn and Cu especially in the SS treatments. Zn in pakchoi was not only in relationship to ΔEXCH and ΔCAR forms but also in ΔOM forms in the sludge-soil mixtures. Tissue content of Cu in pakchoi grown on SC-soils could not be predicted by ΔEXCH. These correlation rates between Zn and Cu accumulation in pakchoi and variation of different fractions increased with time, which might indicate that sludges represented stronger impacts on the plant in long-term land application.     

Intraspecific differences in effects of co-contamination of cadmium and

A hydroponic experiment was carried out to study intraspecific differences in the effects of different concentrations of cadmium (Cd)(0-10 mg/L) and arsenate (As(V)) (0-8 mg/L) on the growth parameters and accumulation of Cd and As in six wheat varieties Jing-9428, Duokang-1, Jingdong-11, Jing-411, Jingdong-8 and Zhongmai-8. The endpoints of wheat seedlings, including seed germination,biomass, root length and shoot height, decreased with increasing the Cd and As concentrations. Significant differences in seed germination, biomass, root length, shoot height and the accumulation of Cd and As were observed between the treatments and among the varieties (p ＜ 0.05). The lethal dosage 50% were about 20, 80, 60, 60, 80 and 20 mg As/L for Jing-9428, Duokang-1, Jingdong-11,Jing-411, Jingdong-8 and Zhongmai-8, respectively, and the corresponding values for Cd were about 30, 80, 20, 40, 60 and 10 mg Cd/L, respectively. Among the six varieties, Duokang-1 was found to be the most resistant to Cd and As toxicity, and Zhongmai-8 was the most sensitive to Cd and As co-contamination. The resistance of the six varieties was found dependant on the seedling uptake of Cd and As. Duokang-1 was the most suitable for cultivation in Cd and As co-contaminated soils.     

Removal of heavy metals from sewage sludge by low costing chemical method and recycling in agriculture

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Isolation and preliminary characterization of a 3-chlorobenzoate degrading bacteria

A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several （halo）aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no （chloro）catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.     

Single and joint effects of pesticides and mercury on soil urease

The influence of two pesticides including chlorimuron-ethyl and furadan and mercury （Hg） on urease activity in 4 soils （meadow burozem and phaeozem） was investigated. The soils were exposed to various concentrations of the two pesticides and Hg individually and simultaneously. Results showed that there was a close relationship between urease activity and organic matter content in soil. Chlorimuron-ethyl and furadan could both activate urease in the 4 soils. The maximum increment of urease activity by chlorimuronethyl was up to 14%-18%. There was almost an equal increase （up to 13%-21%） in the urease activity by furadan. On the contrary, Hg markedly inhibited soil urease activity. A logarithmic equation was used to describe the relationship （P〈0.05） between the concentration of Hg and the activity of soil urease in the 4 tested soils. Semi-effect dose （ED50） values by the stress of Hg based on the inhibition of soil urease in the 4 soils were 88, 5.5, 24 and 20 mg/kg, respectively, according to the calculation of the corresponding equations. The interactive effect of chlorimuron-ethyl or furadan with metal Hg on soil urease was mainly synergic at the highest tested concentrations.