Effects of increased elevation and macro- and micronutrient additions onSpartina alterniflora transplant success in salt-marsh dieback areas in Louisiana

Spartina alterniflora was transplanted into dieback areas of a salt marsh in southeast Louisiana at two elevations (ambient and +30 cm) with and without macro- (N, P, and K) and micronutrient (Fe, Mn, Cu, and Zn) additions to determine if transplant success is dependent on increasing elevation or nutrients.Spartina alterniflora transplanted into elevated plots had more than twice the above- and belowground biomass as compared to nonelevated plots after three months of growth. Additionally, there was significantly more vegetative reproduction (greater culm density and number of newly produced culms) in elevated plots as compared to plots at ambient elevation. Macronutrient additions increased culm densities only in elevated plots.Spartina alterniflora transplanted into nonelevated plots had lower survival rates even when transplants received nutrient additions. These results suggest thatS. alterniflora may be transplanted successfully into degraded salt-marsh areas if elevation is increased. The addition of nutrients without a concomitant increase in elevation is not sufficient for transplant success.     

An Approach Toward Understanding Wildlife-Vehicle Collisions

Among the most conspicuous environmental effects of roads are vehicle-related mortalities of wildlife. Research to understand the factors that contribute to wildlife-vehicle collisions can be partitioned into several major themes, including (i) characteristics associated with roadkill hot spots, (ii) identification of road-density thresholds that limit wildlife populations, and (iii) species-specific models of vehicle collision rates that incorporate information on roads (e.g., proximity, width, and traffic volume) and animal movements. We suggest that collision models offer substantial opportunities to understand the effects of roads on a diverse suite of species. We conducted simulations using collision models and information on Blanding’s turtles (Emydoidea blandingii), bobcats (Lynx rufus), and moose (Alces alces), species endemic to the northeastern United States that are of particular concern relative to collisions with vehicles. Results revealed important species-specific differences, with traffic volume and rate of movement by candidate species having the greatest influence on collision rates. We recommend that future efforts to reduce wildlife-vehicle collisions be more proactive and suggest the following protocol. For species that pose hazards to drivers (e.g., ungulates), identify collision hot spots and implement suitable mitigation to redirect animal movements (e.g., underpasses, fencing, and habitat modification), reduce populations of problematic game species via hunting, or modify driver behavior (e.g., dynamic signage that warns drivers when animals are near roads). Next, identify those species that are likely to experience additive (as opposed to compensatory) mortality from vehicle collisions and rank them according to vulnerability to extirpation. Then combine information on the distribution of at-risk species with information on existing road networks to identify areas where immediate actions are warranted.     

Cumulative Impacts of Dock Shading on <Emphasis Type="Italic">Spartina alterniflora</Emphasis> in South Carolina Estuaries

Salt marshes dominated by Spartina alterniflora and the associated networks of tidal creeks that drain them are characteristic geographical features of southeastern estuaries, important nursery habitat areas, and preferred sites for residential development. As the size of the coastal population increases, so has the number of requests for dock permits. With each new request for a dock permit, public concerns about the cumulative environmental impacts of dock proliferation on the coastal environment have increased. The objective of this particular study was to evaluate the impacts of shading by dock structures on stem densities of S. alterniflora in South Carolina coastal marshes. Shading impacts under individual docks were extrapolated to the tidal creek (local), county, and statewide scales. Dock structures were sampled both under and next to the walkway in the Charleston Harbor area of South Carolina. The density of S. alterniflora under docks was significantly lower than that which occurred next to the docks (i.e., 5 m away) for the short-form, tall-form, and both forms combined. We estimated that shading effects from dock structures in South Carolina decreased the stem density of S. alterniflora by 71%. Dock shading effects were small when evaluated from the perspective of the amount of marsh that occurs within specific tidal creeks (0.03–0.72%), in coastal counties at a maximum dock length (0.01–0.98%), or statewide (0.01–0.13%) at a maximum dock length. However, approximately 7,000 docks have been permitted over the last decade, resulting in a loss of salt marsh equivalent to 60 ha.1Denise M. Sangers present address: Office of Ocean and Coastal Resource Management, South Carolina Department of Health and Environmental Control, 1362 McMillan Avenue, Suite 400 Charleston, South Carolina 29405, USA. 2 A. Frederick Hollands present address: Hollings Marine Laboratory, National Oceanic and Atmospheric Administration, 331 Fort Johnson Road, Charleston, South Carolina 29412, USA.     

Managing Tree-of-Heaven (Ailanthus altissima) in Parks and Protected Areas: A Case Study of Rondeau Provincial Park (Ontario, Canada)

The Carolinian Life Zone in southwestern Ontario, Canada is valued because it represents an almost disjunct ecosystem (i.e., one that is typical of the mid-Atlantic United States, rather than the rest of Canada or the nearby states in the United States). The landscape of the Carolinian Life Zone has undergone dramatic transformation, especially in recent decades as agriculture, urbanization, and recreation have intensified. One of the most apparent changes is the invasion of exotic plant species that exacerbates the need for mass restoration efforts. Within the Carolinian Life Zone, Rondeau Provincial Park has experienced an influx of nonindigenous, invasive species in recent years. Tree-of-heaven (Ailanthus altissima) is one example. The infestation is still relatively localized to (mainly) the park, slowly spreading, and manageable as long as something is done immediately. We examined the effects of hand-pulling and mulching, cut stump and glyphosate application, cut stump alone, and the EZJect Capsule Injection System (using glyphosate) on the management of A. altissima within the park. Cut stump and glyphosate treatment was most effective and efficient in its control of young A. altissima shoots because it limits disturbance and has acceptable capital and operating costs. Hand-pulling and mulching was the second choice, mainly because of the risk of additional disturbance that increased shoot densities 1 year after treatment. Cut stump alone was not effective, worsened the infestation, and is not recommended for this species. The EZJect system was effective at managing mature, seed-producing shoots, although the somewhat higher capital costs mean that the system probably should be purchased for management of several invasive tree species to make it more cost-effective.     

Diversity, Seasonality, and Context of Mammalian Roadkills in the Southern Great Plains

Thousands of mammals are killed annually from vehicle collisions, making the issue an important one for conservation biologists and environmental managers. We recorded all readily identifiable kills on or immediately adjacent to roads in the southern Great Plains from March 2004–March 2007. We also recorded distance traveled, whether a road was paved or divided, the number of lanes, and prevailing habitat. Surveys were opportunistic and were conducted by car during conditions of good visibility. Over our 239 surveys and >16,500 km traveled, we recorded 1412 roadkills from 18 different mammal species (size ranged from Sciurus squirrels to the white-tailed deer, Odocolieus virginianus). The overall kill rate was 8.50 / 100 km. Four species were prone to collisions: the Virginia opossum (Didelphis virginiana), nine-banded armadillo (Dasypus novemcinctus), striped skunk (Mephitis mephitis), and northern raccoon (Procyon lotor). Together they accounted for approximately 85% (1198) of all roadkills. Mortality rate differed significantly between 2- and 4-lane roads (8.39 versus 7.79 / 100 km). Kill rates were significantly higher on paved versus unpaved roads (8.60 versus 3.65 / 100 km), but did not depend on whether a road was divided. Roadkills were higher in spring than in fall (1.5×), winter (1.4×), or summer (1.3×). The spring peak (in kills / 100 km) was driven chiefly by the armadillo (2.76 in spring/summer versus 0.73 in autumn/winter) and opossum (2.65 versus 1.47). By contrast, seasonality was dampened by a late winter/early spring peak in skunk mortalities, for which 41% occurred in the 6-week period of mid-February through March. The raccoon did not exhibit a strong seasonal pattern. Our data are consistent with dispersal patterns of these species. Our results underscore the high rate of highway mortality in the southern plains, as well as differences in seasonality and road type that contribute to mortality. Conservation and management efforts should focus on creating underpasses or using other means to reduce roadkill rates.     

Exclusion by Inclusion? On Difficulties with Regard to an Effective Ethical Assessment of Patenting in the Field of Agricultural Bio-Technology

In order to take ethical considerations of patenting biological material into account, the so-called “ordre public or morality clause” was implemented as Article 6 in the EC directive on the legal protection of biotechnological inventions, 98/44/EC. At first glance, this seems to provide a significant advantage to the European patent system with respect to ethics. The thesis of this paper argues that the ordre public or morality clause does not provide sufficient protection against ethically problematic uses of the patent system within the area of life. On the contrary, there are worrisome obstacles to any effective and comprehensive critical analysis of the ethical aspects of bio-patenting, especially in the field of agriculture. These obstacles can be seen as indirect consequences of the implementation of ethical considerations in form of the ordre public and morality clause in the EC Directive. Therefore, Article 6 of the EC Directive on the legal protection of biotechnological inventions seems to ultimately weaken the position of ethics in the debate concerning bio-patenting because the ordre public and morality clause is usually interpreted in an exclusively bio-ethical way in the sense of an “intrinsic ethics,” which is primarily interested in questions regarding the moral status of particular entities. It is argued that an important cause of this phenomenon is that the decisive reasons against bio-patenting are concerns of social ethics, and not bio-ethics.     

Conversion of temperate forests into heaths: Role of ecosystem disturbance and ericaceous plants

Fire and logging in nutrient-poor temperate forests with certain ericaceous understory plants may convert the forests into heaths. The process of disturbance-induced heath formation is documented by using examples ofCalluna in western Europe,Kalmia in Newfoundland, andGaultheria (salal) in coastal British Columbia. In a cool, temperate climate, rapid vegetative growth ofCalluna, Kalmia, and salal following disturbance results in increasing organic accumulation (paludification), nutrient sequestration, soil acidification, and allelochemicals. These are thought to be the main reasons to conifer regeneration failure in disturbed habitats. If continuation in forest is a land-use objective, then temperate forests with an ericaceous understory should not be logged unless effective silvicultural methods are devised to control the ericaceous plants and restore forest regeneration. Preharvest vegetation control may be considered as an option. Failure to control the understory plants may lead to a long-term vegetation shift, from forest to heathland, particularly in nutrient-poor sites. Successful methods of controllingKalmia andGaultheria, however, have yet to be developed. While theKalmia- andGaultheria- dominated heathlands are undesirable in Canada and the Pacific Northwest, a wide range ofCalluna heathlands of western Europe are being conserved as natural and seminatural vegetation.     

Evaluation of tidal marsh restoration: Comparison of selected macroinvertebrate populations on a restored impounded valley marsh and an unimpounded valley marsh within the same salt marsh system in Connecticut,USA

Macroinvertebrates were examined on an impounded valley marsh in Stonington, Connecticut, that has changed from aTypha-dominated system to one with typical salt-marsh vegetation during 13 years following the reintroduction of tidal exchange. Animal populations on this restored impounded marsh were evaluated by comparing them with populations on a nearby unimpounded valley marsh of roughly the same size. Populations of the high marsh snail,Melampus bidentatus Say, were quantitatively sampled along transects that extended from the water-marsh edge to the upland; those of the ribbed mussel,Geukensia demissa Dillwyn, were sampled in low marsh areas on transects along the banks of creeks and mosquito ditches. The occurrence of other marsh invertebrates also was documented, but their abundance was not measured. The mean density ofMelampus was 332±39.6 SE/m² on the restored impounded marsh and 712±56.0 SE/m² on the unimpounded marsh. However, since snails were larger on the restored impounded marsh, the difference in snail biomass was less pronounced than the difference in snail density. MeanMelampus biomass was 4.96±0.52 SE g dry wt/m² on the restored impounded marsh and 6.96±0.52 SE g dry wt/m² on the unimpounded marsh. On the two marshes, snail density and biomass varied in relation to plant cover and other factors. The density and biomass ofGeukensia at the edge of the marsh were comparable on the restored impounded and unimpounded marshes. Mean mussel densities ranged from 80 to 240/m² and mean mussel biomass varied from 24.8–64.8 g dry wt/m² in different low marsh areas. In contrast, below the impoundment dike, meanGeukensia density was 1100±96.4 SE/m² and meanGeukensia biomass was 303.6±33.28 SE g dry wt/m². A consideration of all available evidence leads to the conclusion that the impounded marsh is in an advanced phase of restoration.     

Model-Based Assessment of Aspen Responses to Elk Herbivory in Rocky Mountain National Park,USA

In Rocky Mountain National Park (RMNP), aspen (Populus tremuloides Michx.) has been observed to be declining on elk (Cervus elaphus nelsoni) winter range for many decades. To support elk management decisions, the SAVANNA ecosystem model was adapted to explore interactions between elk herbivory and aspen dynamics. The simulated probability of successful vegetative regeneration for senescent aspen stands declines sharply when elk densities reach levels of 3–5 elk/km², depending on model assumptions for the seasonal duration of elk foraging activities. For aspen stands with a substantial component of younger trees, the simulated regeneration probability declines more continuously with increasing elk density, dropping below 50% from densities at 8–14 elk/km².At the landscape scale, simulated aspen regeneration probability under a scenario of extensive seasonal use was little affected by elk population level, when this level was above 300–600 elk (25%–50% current population) over the ca. 107 km² winter range. This was because elk distribution was highly aggregated, so that a high density of elk occupied certain areas, even at low population levels overall. At approximately current elk population levels (1000–1200 elk), only 35%–45% of senescent aspen stands are simulated as having at least a 90% probability of regeneration, nearly all of them located on the periphery of the winter range. Successful management for aspen persistence on core winter range will likely require some combination of elk population reduction, management of elk distribution, and fencing to protect aspen suckers from elk browsing.     

Photosensitized Inactivation of Cyst Forms of the Freshwater Amoeba <Emphasis Type="Italic">Vahlkampfia hartmanni</Emphasis> by Aluminium (III) Phthalocyanine Tetrasulfonate

Summary In this work we attained a successful cysticidal effect against Vahlkampfia hartmanni cysts using mild to moderate experimental conditions, regarding light energy delivered and concentrations of aluminium phthalocyanine tetrasulfonate (AlPCS4). The dark and phototoxic activity of AlPCS4 towards the cyst forms of Vahlkampfia hartmanni, a freshwater amoeba previously isolated from keratitic patients, were tested. The photosensitized deactivation of the cyst form of this amoeba depends mainly on the concentration of AlPCS4 and the light fluence rate as well as the total fluence delivered during the irradiation process. Upon irradiation of the cyst suspension at 100 mW/cm² for 10 minutes in the presence of 3 μ M AlPCS4, a complete photodamaging effect on the cysts was induced. The photodamaging effect on Vahlkampfia cysts induces a state of inability of the cysts to excyst, vacuolation of the inner contents as well as a significant damage of the outer cyst wall upon microscopic examination.     

Effect of stocking density and food quality on the growth and fecundity of an epigeic earthworm (<Emphasis Type="Italic">Eisenia fetida</Emphasis>) during vermicomposting

Physico-chemical characteristics of the feed and optimum worm density are important parameters for the efficient working of a vermicomposting system. Overcrowding of worms can affect the efficiency of a vermicomposting system even if all other parameters have been optimized. This article reports the effect of stocking density and feed quality on the growth and fecundity of Eisenia fetida under laboratory conditions. The feed mixtures contained cow dung and textile mill wastewater sludge in different ratios. Three feed mixtures and five stocking rates (1, 2, 4, 8, and 12) were tested for 12 weeks. The results showed that E. fetida growth rate was faster at higher stocking densities; however, biomass gain per worm was faster at lower stocking densities. Sexual maturity was attained earlier at higher stocking densities. Growth rate was highest in 100% cow dung at all the stocking densities when compared to textile mill wastewater sludge containing feed mixtures. A worm population of 27–53 worms per kg of feed was found to be the most favorable stocking density.     

Effectiveness of Management Interventions to Control Invasion by Rhododendron ponticum

Rhododendron ponticum is an invasive species in many countries, including the United Kingdom, Ireland, Belgium, and France. It poses a serious threat to native flora and fauna, as it is capable of altering entire seminatural communities through its vigorous spread. Control is essential if the conservation value of some communities, such as oak woodland and lowland heath, are to be successfully maintained. Commonly used interventions are herbicide application, herbicide application postcut, and cutting (manual or mechanical) alone. Various techniques have been developed to apply these interventions, but often retreatment of the area is required, increasing the cost of control. Here, we evaluate the effectiveness of some commonly used interventions for R. ponticum control using a systematic review methodology. Eleven studies provided data for statistical analysis. Meta-analyses of captured data show that postcut application of the herbicide Glyphosate or applying the herbicides Metsulfuron-methyl or Imazapyr (no cut) can effectively reduce a R. ponticum stand. There is insufficient available experimental evidence for effectiveness of any other intervention. The systematic review process has demonstrated the lack of replicated studies with controls or long-term monitoring and increases the call for more rigorous monitoring of all conservation management interventions. The quality of experimental evidence of the effectiveness of some interventions contrasts with the acceptance of their use through dissemination of experience. The collection and objective review of experience will require active collaboration of organizations concerned with R. ponticum control.     

Use of sequential sampling of amphipod abundance to classify the biotic integrity of acid-sensitive lakes

A sequential sampling program using previously published zoobenthos data is described for ubiquitous, cost-effective biomonitoring of the effects of lake acidification. Spring densities of the littoral amphipodHyalella azteca are quantitatively sorted into five abundance catagories. An essential step in the a priori definition of decision criteria is the stratification of proportional densities per unit macrophyte biomass in relation to aqueous total phosphorus concentrations. Density rankings were related to lake acidity and to detailed lake-specific information on patterns ofHyalella life history and acid tolerance. Incorporation ofHyalella abundance as a potential metric for the assessment of the biological integrity of acid-sensitive waters is recommended. The temporal integration of transient changes in spring meltwater chemistry is an important rationale for the development of such biomonitoring procedures.     

Sequential sampling: A cost-effective approach for monitoring benthic macroinvertebrates in environmental impact assessments

Sequential sampling is a method for monitoring benthic macroinvertebrates that can significantly reduce the number of samples required to reach a decision, and consequently, decrease the cost of benthic sampling in environmental impact assessments.Rather than depending on a fixed number of samples, this analysis cumulatively compares measured parameter values (for example, density, community diversity) from individual samples, with thresholds that are based on specified degrees of precision.In addition to reducing sample size, a monitoring program based on sequential sampling can provide clear-cut decisions as to whethera priori-defined changes in the measured parameter(s) have or have not occurred. As examples, sequential sampling programs have been developed to evaluate the impact of geothermal energy development on benthic macroinvertebrate diversity at The Geysers, California, and for monitoring the impact of crude oil contamination on chironomid midge [Cricotopus bicinctus (Meigen) andC. mackenziensis Oliver] population densities in the Trail River, Northwest Territories, Canada.     

Influence of Landfill Gas on the Microdistribution of Grass Establishment Through Natural Colonization

Many revegetated landfills have poor cover including bare areas where plants do not grow. This study, on the Bisasar Road Landfill site in South Africa, assessed grass species preferences to microhabitat conditions in a mosaic of patches of well-established grassed areas and bare, nonvegetated areas. Factors, including soil CO₂, CH₄, O₂, nutrients, and other general soil conditions, were measured in relation to species distribution and grass biomass in the field. Cynodon dactylon was the dominant grass in the established grass areas but was less abundant in the areas bordering the bare areas where Paspalum paspalodes and Sporobolus africanus were common. A number of soil factors measured were significantly correlated with grass biomass and these included Mg, Ca, Zn, Mn, K, temperature, moisture, and CO₂. However, a laboratory bioassay using the growth of C. dactylon with soils removed from the landfill indicated that there were no differences in the soils from the bare areas and those that supported high plant biomass. Thus, no nutrient deficiency or chemical toxicity was inherent in the soil in the laboratory. The results of the field investigation and bioassay indicated that soil CO₂ as a result of landfill gas infiltration into the root zone was probably the main factor causing bare areas on the landfill where no grass species could colonize and grow and that C. dactylon was more sensitive to elevated soil CO₂ than other grass species such as P. paspalodes and S. africanus.     

An Analysis of Spatial Clustering and Implications for Wildlife Management: A Burrowing Owl Example

Analysis tools that combine large spatial and temporal scales are necessary for efficient management of wildlife species, such as the burrowing owl (Athene cunicularia). We assessed the ability of Ripley’s K-function analysis integrated into a geographic information system (GIS) to determine changes in burrowing owl nest clustering over two years at NASA Ames Research Center. Specifically, we used these tools to detect changes in spatial and temporal nest clustering before, during, and after conducting management by mowing to maintain low vegetation height at nest burrows. We found that the scale and timing of owl nest clustering matched the scale and timing of our conservation management actions over a short time frame. While this study could not determine a causal link between mowing and nest clustering, we did find that Ripley’s K and GIS were effective in detecting owl nest clustering and show promise for future conservation uses.     

Riverbanks and the law: The arbitrary nature of river boundaries in New South Wales,Australia

Key aspects of environmental management exist within a legislative framework. The Rivers and Foreshores Improvement Act 1948 (NSW) and several Regional Environmental Plans created under the Environmental Planning and Assessment Act 1979 (NSW) make reference to ‘the top of the bank’ for defining areas of protected land adjacent to rivers, within which development consent may be required. It is an arbitrary term and its use within the Rivers and Foreshores Improvement Act 1948 (NSW) leads to confusion. This paper examines the range of definitions of ‘the top of the bank’ in respect of natural watercourses and aims to provide a more lucid and effective definition that will clarify existing ambiguities in legal interpretation. The paper examines the historical origins of the phrase ‘top of the bank’, finding that stereotyped Eurocentric views of what a river ‘should look like’ have impaired the legal definition for Australian rivers, thereby influencing common law and the development of statutory definitions. Judicial applications of the phrase ‘top of the bank’ are examined from a geomorphological perspective, demonstrating the misconceptions of the term in a legal context. The paper identifies the existence of widespread support for the need to protect land adjacent to rivers in the interests of environmental, economic and social sustainability. It concludes by calling for legislative reform that is both tailored to the individual site and consistent with overarching goals at the catchment scale.     

Short- and Longer-Term Effects of Fire and Herbivory on Sagebrush Communities in South-Central Montana

To better understand the role of herbivory and fire as potential disturbance processes in sagebrush communities, we examined responses of a grazing ungulate, elk (Cervus elaphus), following prescribed burning of sagebrush (Artemisia tridentata ssp. vaseyana) in south-central Montana (USA.) with concurrent monitoring of changes in plant production, nutritional quality, and community diversity from 1989–1999. Burning transformed low-diversity, sagebrush-dominated communities into high-diversity, graminoid-forb communities that persisted for 10 years without significant reestablishment of sagebrush. Elk increased use of burned sites one year after burning, but elk use returned to pre-burn levels over the next two to nine years. Forage biomass and nutritional quality declined after initial increases that coincided with increased elk use. Increases in elk use appeared to be influenced by increases in combined graminoid and forb production and changes in structural vegetation characteristics that permitted greater foraging efficiency. Declines in use were associated with loss of nutritional enhancement and declines in combined graminoid and forb production. Managers may observe only short-term responses from grazing ungulates to prescribed fire in sagebrush communities, but can expect longer-term increases in plant diversity and establishment of graminoid-forb communities.     

Use of Artificial Roost Structures by Bats at the Indianapolis International Airport

From 1992–1996, 3204 artificial roosts of 9 types were placed in woodlots near Indianapolis International Airport in an effort to provide habitat for the federally-endangered Indiana myotis (Myotis sodalis) and to determine the feasibility of using these structures to manage bats in a rapidly developing suburban area. We surveyed these structures at least annually during 1992–1999 and found only northern myotis (Myotis septentrionalis) regularly using the structures. Four other species were occasionally found using structures including big brown bats (Eptesicus fuscus, n = 14 individuals), little brown myotis (Myotis lucifugus, n = 2), Indiana myotis (Myotis sodalis, n = 2), and one silver-haired bat (Lasionycteris noctivagans). Single, triple, and Missouri-style batboxes were almost always used, rather than the six other types of experimental roosts that had been in place. However, after 10 years in place, it appears that Indiana bats are acclimated to boxes, as 6 of them were being used rather regularly by Indiana myotis. Bat boxes can provide roosting habitat for some species under conditions where few suitable roosts exist, but assuring an abundance of natural habitats is usually more desirable for conservation of tree-roosting bats.