Assessment of cadmium accumulation,toxicity, and tolerance in Brassicaceae and Fabaceae plants—implications for phytoremediation

This study, based on a greenhouse pot culture experiment conducted with 15-day-old rapeseed (Brassica campestris L. cv. Pusa Gold; family Brassicaceae) and moong bean (Vigna radiata L. Wilczek cv. Pusa Ratna; family Fabaceae) plants treated with cadmium (Cd) concentrations (0, 50, and 100 mg kg^?1 soil), investigates their potential for Cd accumulation and tolerance, and dissects the underlying basic physiological/biochemical mechanisms. In both species, plant dry mass decreased, while Cd concentration of both root and shoot increased with increase in soil Cd. Roots harbored a higher amount of Cd (vs. shoot) in B. campestris, while the reverse applied to V. radiata. By comparison, root Cd concentration was higher in B. campestris than in V. radiata. The high Cd concentrations in B. campestris roots and V. radiata shoots led to significant elevation in oxidative indices, as measured in terms of electrolyte leakage, H₂O₂ content, and lipid peroxidation. Both plants displayed differential adaptation strategies to counteract the Cd burden-caused anomalies in their roots and shoots. In B. campestris, increasing Cd burden led to a significantly decreased reduced glutathione (GSH) content but a significant increase in activities of GSH reductase (GR), GSH peroxidase (GPX), and GSH sulfotransferase (GST). However, in V. radiata, increasing Cd burden caused significant increase in GSH content and GR activity, but a significant decline in activities of GPX and GST. Cross talks on Cd burden of tissues and the adapted Cd tolerance strategies against Cd burden-accrued toxicity indicated that B. campestris and V. radiata are good Cd stabilizer and Cd extractor, respectively, wherein a fine tuning among the major components (GR, GPX, GST, GSH) of the GSH redox system helped the plants to counteract differentially the Cd load-induced anomalies in tissues. On the whole, the physiological/biochemical characterization of the B. campestris and V. radiata responses to varying Cd concentrations can be of great help in elaborating the innovative plant-based remediation technologies for metal/metalloid-contaminated sites.     

Evolution of particles in the plumes of coal-fired power plants—II. A numerical model and comparisons with field measurements

A three-dimensional numerical model is described which considers the effects of advection, diffusion, coagulation, gravitational settling, gas-to-particle (g-to-p) conversion and interactions with the ambient air on the evolution with time of particle size distributions in power plant plumes. From inputs of an atmospheric temperature, humidity and wind sounding, and the concentrations of particles and trace gases emitted from a power plant and in the ambient air, the model provides predictions of the particle size distributions, total particle concentrations, concentrations of trace gases, g-to-p conversion rates and the light-scattering coefficient of the particles at points in the plume downwind of the stack.Comparisons of the model predictions with airborne measurements obtained in the plumes from two coal-fired power plants show that the model is capable of predicting many of the principal features of the evolution of the particle size distributions and related parameters for transit distances up to at least 30 km. The model predicts maximum g-to-p conversion rates at the edges of the plumes where the predicted g-to-p conversion rates are comparable to values derived from field measurements. Elsewhere in the plumes the predicted g-to-p conversion rates are about an order of magnitude lower than the average values deduced from field measurements.     

Socio-economic and environmental impacts of COVID-19 pandemic in Pakistan—an integrated analysis

Environmental Science and Pollution Research - The COVID-19 pandemic has affected about 210 countries with more than 67 million confirmed cases and over 1.5 million deaths across the globe...     

Trends in atmospheric sulfur and nitrogen species in the eastern United States for 1989–1995

Emission reductions were mandated in the Clean Air Act Amendments of 1990 with the expectation that they would result in major reductions in the concentrations of atmospherically transported pollutants. This paper investigates the form and magnitude of trends from 1989 to 1995 in atmospheric concentrations of sulfur dioxide, sulfate, and nitrogen at 34 rural sites in the eastern US. Across all sites, there is strong evidence of statistically significant declining trends in sulfur dioxide (median change of -35%) and sulfate concentrations (median change of -26%). In general, trends in nitrogen concentrations were not as pronounced (median change of -8%) as trends in the sulfur compounds. A regional estimate of trend for a cluster of sites in the Ohio River valley showed close correspondence between declining sulfur dioxide concentrations (-35%) and changes in sulfur dioxide emissions (-32%) in this region.     

Development of an In-Line System for the Analysis of 4,4′-DDT in Water

An in-line system for trace persistent organic pollutants (POPs) in water was developed by using a laboratory-made hollow fiber membrane (HFM) unit connected with a high-resolution gas chromatograph-mass spectrometer (HRGC-MS). The semivolatile organic compound, 4,4′-Dichlorodiphenyl trichloroethane (4,4′-DDT), was chosen as a representative of a persistent organic compound. The synthetic water contaminated with 4,4′-DDT was passed through the HFM unit, the extraction occurred by the analyte pervaporated and permeated, then stripped into HRGC-MS. Several factors were investigated for the high extraction efficiency. The best performance was obtained at sample and stripping gas flow rates of 6 and 9 mLmin^?1, respectively, and desorption temperature of 60°C. At this temperature, the diffusion rate was enhanced by 15 times over 25°C. A wide linear dynamic range was obtained, i.e., 0.10–1.0 mgL^?1, with a limit of detection (LOD) of 90 μgL^?1. The extraction efficiency of 4,4′-DDT in real water samples was in the range of 83–94%. Real water samples were analyzed and 0.6 μgL^?1 of 4,4′-DDT was found in unregistered bottled water and 7.0 μgL^?1 in tap water.     

Seawater intrusion vulnerability in the coastal aquifers of southern India—an appraisal of the GALDIT model,parameters’ sensitivity,and hydrochemical indicators

An appraisal of seawater intrusion into the coastal aquifers is one of the major issues for groundwater resource management. The GALDIT model applies to the analysis of multiple parameters using systematic GIS techniques for mapping and assessment of seawater intrusion vulnerability. It demarcates the mapping of potential vulnerability that shows a higher vulnerability to seawater intrusion in various parts of the coast and the estimated vulnerability index value of 7.50 and 9.64. An area of 33.0 km² spread in the low-lying coastal area comprising estuaries, salt marshes, and saltpans shows the high vulnerability condition with an estimated vulnerability value of 6.42–7.50. An area of 73.20 km² spread over coastal and alluvial plains experiences moderate vulnerability (temporal salinity in the groundwater sources) with an estimated vulnerability index value of 5.46–6.42. Aquifers underlying coastal uplands (hard rock formations) and some parts of accretionary beaches (2.05 km²) are relatively protected fresh groundwater sources, wherein the estimated vulnerability index is 4.55–5.46. The vulnerability mapping of the GALDIT model using hydrochemical analysis of primary groundwater parameters such as TDS, Cl^?, HCO₃, and Cl^?/HCO₃ ratio is validated. Higher concentration of TDS (2637–4162 mg/l) and Cl^? (1268–2347 mg/l) is taken for the areas falling under higher vulnerability to seawater intrusion, especially in the placer mining sites and coastal areas facing erosion. Similarly, the groundwater sources of the low-lying areas including estuaries, salt marshes, saltpans, and backwater were noted to have higher values of Cl^?/HCO₃ with a rationality of 9.87–12.18. Hydrological facies shows the highest concentration of NaCl in the groundwater sources within the proximity of eroded beaches, saltwater bodies, and sand mining areas. A hydrochemical facies evolution (HFE) diagram represents the hydrochemical facies of groundwater elements that shows an intrusion of seawater into the coastal aquifers underlying the very high vulnerable zones. Higher bicarbonate concentration (233–318 mg/l) is noticed in the upland areas and some parts of dunes and accreted beaches, sandy coasts, and uplands. Vulnerability analysis reveals that those areas near saltwater bodies and eroding coasts are prone to lateral and vertical diffusion of saltwater. The geodatabase developed through such modeling studies can help in planning and developing activities for sustainable groundwater resource management in coastal areas.

     

Is bark pH more important than tree species in determining the composition of nitrophytic or acidophytic lichen floras?

To study the pH preference of epiphytic lichens, the bark pH of Fraxinus, Tilia, Quercus and Ulmus trees in an urban environment was measured using a flat surface electrode. The total number of trees was 253. A survey was made of the lichens in a 40 × 40 cm quadrat surrounding the pH measurement point. Our data analysis using multivariate and univariate statistical techniques indicates that the tree species is the most important factor influencing lichen colonisation, and that bark pH alone is of less importance. We hypothesize that the changed pollution climate, with strong decreases in both sulphur dioxide and ammonia concentrations over the past two decades and a concomitant general increase in bark pH, has made epiphytes less sensitive to pH.     

Assessment of Needs for Technical Information in EPA’s Hazardous and Solid Waste Programs

Headquarters managers and Regional staff in the U.S. Environmental Protection Agency’s hazardous and solid waste programs were surveyed to determine priorities for technical information and guidance among EPA Regional staff, state hazardous waste management staff, EPA contractors, and the regulated community. The survey also examined delivery systems effective for EPA Regional staff. The fifteen highest ranked technical needs for RCRA, Superfund, and UST reflect a pervasive interest in hazardous waste remediation. Top priority technical needs focus on establishing cleanup levels, subsurface fate and transport, field monitoring and quality assurance, remedy selection, and most importantly, which remedies work and which do not in specific situations. Technical needs of non- EPA audiences are quite similar to those for EPA field staff. Preferences for technology transfer delivery systems are generally for conventional distribution methods (such as print materials and workshops) rather than electronic or video media. Regional staff report serious problems in utilizing technology transfer because of time constraints, insufficient knowledge of available products, insufficient travel funds, and limited access to and training on computers.     

Distribution,persistence, and fate of mexacarbate in the aquatic environment of a mixed‐wood boreal forest

Abstract

The distribution and persistence of aerially applied mexacarbate were studied in a New Brunswick aquatic forest environment after spraying twice at a dosage of 70 g A.l./ha using a fixed‐wing aircraft. Average droplet density (drops/cm²) and ground deposition (g A.1./ha) between the two applications differed considerably. The values for the first and second applications were 1.7 and 0.73, and 5.2 and 2.0, respectively; but the average NMD (20 μm) and VMD (36 μm) for both applications were nearly the same. The maximum 1‐h postspray concentrations of mexacarbate in the stream and pond waters were 0.73 and 18.74 ppb, respectively. Concentrations fell rapidly to below detection limits within 12 h in stream and within 3 d in pond water. Cattails (Typha latifolia), manna grass (Glyceria borealis) and bog moss (Sphagnum sp.) collected from the pond contained peak 1‐h postspray concentrations of 720, 482 and 81 ppb, respectively. The concentration levels decreased rapidly and the average half‐lives of the chemical in them were about 3.9, 8.5 and 2.0 h. Bog moss, stream moss (Fontinalis sp.), watercress (Nasturtium officinalis), buttercup (Ranunculus aquatilis) and green alga (Drapamaldia sp.) sampled from the stream sites did not contain measurable levels of mexacarbate. Also, caged and wild tadpoles (Rana clamitans melanota) from the pond, and brook trout (Salvelinus fontinalis) (caged and wild), Atlantic salmon (Salmo salar) (wild) and mayfly nymphs (Ephemeralla sp.) collected from the stream did not contain any of the material. Mexacarbate was not detected in stream and pond sediments. The demethylated products, 4‐methylamino and 4‐amino‐3,5‐xylyl methylcarbamates and the phenol, 4‐dimethylamino‐3,5‐xylenol, were frequently detected as metabolites in water and in the aquatic plants. The presence of these compounds showed that demethylation and hydrolytic routes are the major metabolic pathways for the dissipation of mexacarbate from these substrates.     

Conservation and development in Amazonian extractive reserves: the case of Alto Juruá

Extractive reserves constitute an innovative approach to match conservation and development objectives, which were originally envisaged as part of a land struggle by forest dwellers in Brazil. In spite of the idea's popularity and the attempts to apply the concept to different tropical regions, there has been little analysis of the combined conservation and development performance of extractive reserve programs. We present a detailed analysis of deforestation and demographic and socioeconomic changes in Alto Juruá, the first extractive reserve created in Brazil in 1990. Forest cover has remained fairly stable. Population has declined slightly, with some internal displacements. The cash economy base has shifted from the original rubber production to a diversified portfolio of agriculture and livestock, and there has been a dramatic rise in nonagrarian income. We conclude that the Reserve represents a very dynamic setting with positive conservation and development outcomes during its first decade.     

Dissolved heavy metal concentrations of the Kralkızı, Dicle and Batman dam reservoirs in the Tigris River basin,Turkey

Water samples were collected at monthly intervals during 1 year of monitoring from Kralk?z?, Dicle and Batman dam reservoirs in the Tigris River basin to assess the concentrations of dissolved heavy metals and to determine their spatial and seasonal variations. The results indicated that dissolved heavy metal concentrations in the reservoirs were very low, reflecting the natural background levels. The lowest total metal concentrations in the three dam reservoirs were detected at sampling sites close to the dam wall. However, the highest total concentrations were observed at sites, which are located at the entrance of the streams to the reservoirs. Fe, Cr and Ni were the most abundant elements in the reservoirs, whereas Cd and As were the less abundant. The mean concentrations of dissolved metals in the dam reservoirs never exceeded the maximum permitted concentrations established by EC (European Community), WHO and USEPA drinking water quality guidelines. All heavy metals showed significant seasonal variations. As, Cd, Cr, Cu, Fe, Ni and Pb displayed higher values in the dry season, while higher values for Zn in the wet season. Cluster analysis grouped all ten sampling sites into three clusters. Clusters 1 and 2, and cluster 3 corresponded to relatively low polluted and moderate polluted regions, respectively. PCA/FA demonstrated the dissolved metals in the dam reservoirs controlled by natural sources.     

Metabolism of acetaminophen (paracetamol) in plants—two independent pathways result in the formation of a glutathione and a glucose conjugate

Background, aim, and scope  Pharmaceuticals and their metabolites are detected in the aquatic environment and our drinking water supplies. The need for high quality drinking water is one of the most challenging problems of our times, but still only little knowledge exists on the impact of these compounds on ecosystems, animals, and man. Biological waste water treatment in constructed wetlands is an effective and low-cost alternative, especially for the treatment of non-industrial, municipal waste water. In this situation, plants get in contact with pharmaceutical compounds and have to tackle their detoxification. The mechanisms for the detoxification of xenobiotics in plants are closely related to the mammalian system. An activation reaction (phase I) is followed by a conjugation (phase II) with hydrophilic molecules like glutathione or glucose. Phase III reactions can be summarized as storage, degradation, and transport of the xenobiotic conjugate. Until now, there is no information available on the fate of pharmaceuticals in plants. In this study, we want to investigate the fate and metabolism of N-acetyl-4-aminophenol (paracetamol) in plant tissues using the cell culture of Armoracia rusticana L. as a model system. Materials and methods  A hairy root culture of A. rusticana was treated with acetaminophen in a liquid culture. The formation and identification of metabolites over time were analyzed using HPLC-DAD and LC–MSⁿ techniques. Results  With LC–MS technique, we were able to detect paracetamol and identify three of its metabolites in root cells of A. rusticana. Six hours after incubation with 1 mM of acetaminophen, the distribution of acetaminophen and related metabolites in the cells resulted in 18% paracetamol, 64% paracetamol–glucoside, 17% paracetamol glutathione, and 1% of the corresponding cysteine conjugate. Discussion  The formation of two independently formed metabolites in plant root cells again revealed strong similarities between plant and mammalian detoxification systems. The detoxification mechanism of glucuronization in mammals is mirrored by glucosidation of xenobiotics in plants. Furthermore, in both systems, a glutathione conjugate is formed. Due to the existence of P450 enzymes in plants, the formation of the highly reactive NAPQI intermediate is possible. Conclusions  In this study, we introduce the hairy root cell culture of A. rusticana L. as a suitable model system to study the fate of acetaminophen in plant tissues. Our first results point to the direction of plants being able to take up and detoxify the model substrate paracetamol. These first findings underline the great potential of using plants for waste water treatments in constructed wetlands. Recommendations and perspectives  This very first study on the detoxification of a widely used antipyretic agent in plant tissues again shows the flexibility of plant detoxification systems and their potential in waste water treatment facilities. This study covers only the very first steps of acetaminophen detoxification in plants; still, there is no data on long-term exposure as well as the possible impact of pharmaceuticals on the plant health and stress defense. Long-term experiments need to be performed to follow the fate of acetaminophen in root and leaf cells in a whole plant system, and to evaluate possible usage of plants for the remediation of acetaminophen from waste water.     

The use of vegetation,bees, and snails as important tools for the biomonitoring of atmospheric pollution—a review

The continuous discharge of diverse chemical products in the environment is nowadays of great concern to the whole world as some of them persist in the environment leading to serious diseases. Several sampling techniques have been used for the characterization of this chemical pollution, although biomonitoring using natural samplers has recently become the technique of choice in this field due to its efficiency, specificity, and low cost. In fact, several living organisms known as biomonitors could accumulate the well-known persistent environmental pollutants allowing their monitoring in the environment. In this work, a review on environmental biomonitoring is presented. The main sampling techniques used for monitoring environmental pollutants are first reported, followed by an overview on well-known natural species used as passive samplers and known as biomonitors. These species include conifer needles, lichen, mosses, bees and their byproducts, and snails, and were widely used in recent research as reliable monitors for environmental pollution.

     

Trace elements exposure influences proximate body composition and antioxidant enzyme activities of the species tilapia and catfish in Burullus Lake—Egypt: human risk assessment for the consumers

The trace elements concentration in the fish estimates the contamination degree in the aquatic environment. These toxic trace elements are transported into the human through the consumption of polluted fish. This study estimated the effect of Cd, Hg, Pb, As, and Al on tilapia species and catfish Clarias gariepinus (six for each species of fish) inhabiting Burullus Lake—Egypt 30° 33′–31° 08′ E and 30° 22′–31° 35′ N. The highest Pb concentrations recorded in the muscle of C. gariepinus 2.29 ± 0.29 μg/g while S. galilaeus was estimated the lowest Hg concentration of 0.54 ± 0.02 μg/g which indicated the presence of contaminants exceeded the limits permitted by FAO/WHO and EC. The maximum mean carbohydrate, lipid, and protein recorded in O. niloticus 18.66, 16.33, and 58.16 mg/g, respectively; moisture in O. aureus 67.33%; and ash 16.41% in O. niloticus. The lowest amount of carbohydrate was recorded in the T. zillii 14.1 mg/g, lipid, and ash in C. gariepinus 11.65 mg/g and 3.375%, respectively. Protein and moisture in the S. galilaeus were 53.75 mg/g and 60.75%, respectively. The results recorded a marked insignificant (P > 0.05) decrease in CAT, GR, and GPx activity in O. niloticus. GSH and SOD activity was an insignificant (P > 0.05) decrease in C. gariepinus. The results concluded that the trace elements concentrations exceed the maximum permissible limits recommended in fish samples set by Egypt, FAO, WHO, and EC. The estimated weekly intake of all elements through consumption of studied fish species inhabiting Burullus Lake by a child (15 kg) in Egypt is well above the PTWI recommended by FAO/WHO, whereas it is well below the PTWI for human consumption by young people (40 kg) and adult person (70 kg), at least in respect of residual levels of studied elements excluding Cd and Hg. Thus, for consumer protection, these fish species are unsafe and have hazardous effects for children, and about youth and adult consumption, caution must be taken to consider individuals eating significant amounts of fish.

     

Derivation of soil values for the path ‘Soil-Soil Organisms’ for metals and selected organic compounds using species sensitivity distributions

BACKGROUND, AIMS AND SCOPE: According to the German Federal Soil Protection Act, the natural function of soil as a habitat for human beings, animals, plants and soil organisms is, among other things, to be protected by deriving soil values for important chemicals regarding their amounts in the environment, their persistence and/or their toxicity. This contribution presents the results of the mathematical derivation of such values for nine metals and ten organic substances from soil ecotoxicological effect values available in the literature for microbial processes, plants and soil invertebrates. MATERIAL AND METHODS: Ecotoxicological data were mostly extracted from published papers and reports and had to originate from valid studies that were performed according to internationally standardised guidelines (e.g. ISO) or were otherwise well documented, plausible and performed according to accepted laboratory practice. As test results, both structural (i.e., effects on mortality, growth or reproduction) and functional (i.e., effects on microbial activity or organic matter breakdown) parameters were included. The derivation of soil values was performed using the distribution based extrapolation model (DIBAEX) and EC(50)s (Effective Concentration) as input data. RESULTS: For 19 compounds, soil values could be calculated. In 18 of these 19 cases clear laboratory ecotoxicological effects (i.e., EC50 values) below the calculated soil value have been found in the literature. DISCUSSION: In those few cases where a comparison with field studies is possible, effects have been observed in the same order of magnitude as the calculated soil values. A comparison with other similar approaches confirmed the plausibility of the calculated values. CONCLUSIONS: The DIBAEX-method is a feasible and widely accepted method for deriving soil values from ecotoxicological input data. Data availability was already satisfactory for some substances, but other substances, especially organics, were only poorly covered. The soil values presented here were based on EC50 input data. However, depending on the protection level aimed at by using soil values in legislation, it might be appropriate to use other input data such as NOECs in the derivation process. RECOMMENDATIONS AND PERSPECTIVES: It is recommended to generate an appropriate number of data for further relevant substances by means of a test battery or multi-species approaches such as terrestrial model ecosystems. These tests should also consider the influence of the bioavailability of substances. A final recommendation for legally binding soil values demands a plausibility check of the mathematically derived values. This should include a comparison with natural background concentrations, soil values for other pathways and soil values used in legislation of other countries. Finally, expert judgement always has to be considered.     

Isoprene and monoterpene emission from the coniferous species Abies Borisii-regis—implications for regional air chemistry in Greece

The emission of isoprene has been studied from a forest of Abies Borisii-regis, a Mediterranean fir species previously thought to emit only monoterpenes. Emission studies from two independent enclosure experiments indicated a standardised isoprene emission rate of (18.4±3.8) μg g_dry-weight⁻¹ h⁻¹, similar in magnitude to species such as eucalyptus and oak which are considered to be strong isoprene emitters. Isoprene emission depended strongly on both leaf temperature (2°C–34°C) and photosynthetically active radiation (PAR) below 250 μmol m⁻² s⁻¹, becoming saturated with respect to PAR above this value. The annual isoprene emission rate was estimated to be (132±29) kT yr⁻¹ for those trees growing within Greece, comparable to current estimates of the total isoprene budget of Greece as a whole, and contributing significantly to regional ozone and carbon monoxide budgets. Monoterpene emission exhibited exponential temperature dependence, with 1,8-cineole, α-pinene, β-pinene and limonene forming the primary emissions. A standardised total monoterpene emission rate of (2.7±1.1) μg g_dry-weight⁻¹ h⁻¹ was calculated, corresponding to an annual monoterpene emission rate of (24±12) kT yr⁻¹. Research was conducted as part of the AEROBIC’97 (AEROsol formation from BIogenic organic Carbon) series of field campaigns.     

Is tourism damaging ecosystems in the Andes? Current knowledge and an agenda for future research

Despite the popularity of tourism and recreation in the Andes in South America and the regions conservation value, there is limited research on the ecological impacts of these types of anthropogenic use. Using a systematic quantitative literature review method, we found 47 recreation ecology studies from the Andes, 25 of which used an experimental design. Most of these were from the Southern Andes in Argentina (13 studies) or Chile (eight studies) with only four studies from the Northern Andes. These studies documented a range of impacts on vegetation, birds and mammals; including changes in plant species richness, composition and vegetation cover and the tolerance of wildlife of visitor use. There was little research on the impacts of visitors on soils and aquatic systems and for some ecoregions in the Andes. We identify research priorities across the region that will enhance management strategies to minimise visitor impacts in Andean ecosystems.

Electronic supplementary material

The online version of this article (doi:10.1007/s13280-014-0550-7) contains supplementary material, which is available to authorized users.