Enumeration of waterborne Escherichia coli with petrifilm plates: comparison to standard methods

Escherichia coli is often monitored in environmental waters as an indicator of the possible presence of human pathogens associated with feces. Petrifilm E. coli/coliform count plates (3M, Minneapolis, MN), previously validated for enumerating E. coli in food, were tested for monitoring E. coli in environmental water. Escherichia coli counts in environmental water samples enumerated with Petrifilm were significantly correlated (R > 0.9; slope = 0.9-1.0; p < 0.001) with counts obtained with three commonly used methods, mTEC (Becton Dickinson, Sparks, MD), m-ColiBlue (Hach, Loveland, CO), and Colilert-18/IDEXX Quanti-Tray 2000 (IDEXX, Westbrook, ME). Blue colonies on Petrifilm plates were most reliably identified as E. coli when accompanied by gas formation, as determined by characterization of the colonies on MacConkey agar plates (PML Microbiologicals, Mississauga, ON, Canada) and by polymerase chair reaction (PCR) with E. coli-specific primers. The main disadvantage of Petrifilm plates for environmental water testing is the small volume (1 mL per sample) that can be tested; however, the plates appear to be suitable for screening and locating sites that exceed criteria for total body and partial body contact. Simplicity of use and storage, reliability, and relatively low cost make Petrifilm plates suitable for volunteer-based and educational water quality monitoring applications, particularly when used as a preliminary screening method to identify problem sites.     

Assessing potential bioavailability of metals in sediments: A proposed approach

Due to anthropogenic inputs, elevated concentrations of metals frequently occur in aquatic sediments. In order to make defensible estimates of the potential risk of metals in sediments and/or develop sediment quality criteria for metals, it is essential to identify that fraction of the total metal in the sediments that is bioavailable. Studies with a variety of benthic invertebrates indicate that interstitial (pore) water concentrations of metals correspond very well with the bioavailability of metals in test sediments. Many factors may influence pore water concentrations of metals; however, in anaerobic sediments a key phase controlling partitioning of several cationic metals (cadmium, nickel, lead, zinc, copper) into pore water is acid volatile sulfide (AVS). In this paper, we present an overview of the technical basis for predicting bioavailability of cationic metals to benthic organisms based on pore water metal concentrations and metal-AVS relationships. Included are discussions of the advantages and limitations of metal bioavailability predictions based on these parameters, relative both to site-specific assessments and the development of sediment quality criteria.     

A practical scientific approach to riparian vegetation rehabilitation in Australia

The clearance of indigenous riparian vegetation and removal of large woody debris (LWD) from streams combined with the planting of exotic plant species has resulted in widespread detrimental impacts on the fluvial geomorphology and aquatic ecology of Australian rivers. Vegetation exerts a significant influence on fluvial geomorphology by affecting resistance to flow, bank strength, sediment storage, bed stability and stream morphology and is important for aquatic ecosystem function. As the values of indigenous riparian vegetation are becoming better recognised by Australian river managers, large amounts of money and resources are being invested in the planting of indigenous riparian vegetation as part of river rehabilitation programs. This paper summarises the results of an investigation into the survival, growth and regeneration rates of a series of trial native riparian vegetation plantings on in-channel benches in the Hunter Valley of southeastern Australia. The trials were poorly designed for statistical analysis and the paper highlights a number of shortcomings in the methods used. As a result, a new approach to riparian vegetation rehabilitation is outlined that promotes the use of scientific principles and understanding. Appropriate species should be selected using a combination of remnant vegetation surveys, historical records, palynology and field trials. A number of important factors should be considered in the rehabilitation of riparian vegetation to achieve worthwhile results. These include flood disturbance, vegetation zonation, vegetation succession, substrate composition, corridor planting width, planting techniques, native plant regeneration, LWD recruitment and adaptive ecosystem management. This approach, if adopted, revised and improved by river managers, should result in greater success than has been achieved by previous riparian vegetation rehabilitation efforts in Australia.     

Measuring forest ecosystem sustainability: A resource accounting approach

A new environmental paradigm has emerged, reflecting a change in the public's understanding of resource sustainability. Forest policy makers need to be better informed about such changes to achieve economic, social, and environmental objectives in a manner that balances human needs and aspirations with ecosystem constraints. As an aid to this task, a forest resource accounting system based on the key concept of natural capital could help reshape forest policies to provide an even wider spectrum of benefits for both present and future generations by maintaining and enhancing the productive capacity of forest capital. Such a resource accounting system would provide a tool for integrating multidimensional information requirements in measuring the health of both forest ecosystems and economic systems. This paper outlines some of the features of this accounting system and proposes and framework that would integrate economic and ecological characteristics of natural resources. Forest resource accounting is urgently needed to achieve the sustainability goals of ecosystem management.     

Responding to Rio: a local authority approach

The concern for sustainable development which has been accentuated by the United Nations Conference on Environment and Development (UNCED), is now being reflected at national and local government levels. In particular, Agenda 21, which was endorsed at the conference, has led to the implementation of a Local Agenda 21 programme. In Britain, extensive guidance and support on the interpretation of sustainable development has been provided by the Local Government Management Board and others. This paper reviews the general response to Local Agenda 21 in Britain and describes one local authority's approach.     

Energy and entropy: a thermodynamic approach to sustainability

Thermodynamics is a basic science that formulates the rules for the conversion of energy and matter from one form into another. It sets the physical limits for the evolution of and the developments in the world around us. In contradiction to the mechanical approach, thermodynamics indicates that economic growth leads to increasing disorder. More specifically, increasing the flows of energy and matter through society, as happens in the process of ongoing industrialization, leads to progressive depletion of available energy and matter or, otherwise stated, to increased entropy. Excessive entropy production is reflected in natural disorders such as the greenhouse effect, ozone holes, environmental pollution, etc. Sustainable development can only be approached by imposing a close to steady-state lifestyle on mankind.     

A biotest for evaluating copper bioavailability to plants in a contaminated soil

This work aimed at defining the optimal conditions for a novel ecotoxicological test designed for evaluating the bioavailability and phytotoxicity of metals to plants. This biotest, which provided easy access to roots, shoots, and rhizosphere soil, was applied to a vineyard calcareous soil that had been contaminated by the application of Cu fungicides. A preliminary hydroponic experiment comparing various levels of solution Cu concentration enabled us to determine the no observable adverse effects concentration (NOAEC), which was in the range 5 to 20 microM total Cu (0.01-0.06 microM free Cu ion) for rape (Brassica napus L. cv. Goeland). For the biotest, rape was grown in hydroponic conditions for 21 d in pots designed so that plants developed a planar mat of roots at the surface of a polyamide mesh. By then, the plants were transferred for 4 or 8 d onto a 1- or 3-mm-thick soil layer that was separated from the root mat by the mesh and connected to a reservoir of nutrient solution or deionized water via a filter paper wick. An 8-d period was the best option as it enabled plant growth to be significant. The use of 1-mm soil thickness was recommended if the biotest aimed at investigating root-induced changes in the rhizosphere. Although it may cause some artifacts, compared with deionized water, nutrient solution provided better standardized conditions for comparing widely differing soil samples. The studied soil did not induce any Cu phytotoxicity in spite of its fairly large total Cu content.     

Generating sustainable towns from Chinese villages: a system modeling approach

The great majority of China's developing towns will be extensions of already existing villages. With the prospect of hundreds of millions of Chinese farmers projected to leave their villages to become industrial workers in new and expanded towns within the next few years, new challenges will be faced. As expansion and modernization progress, this development moves from the traditional village model that operates not far from resource sustainability to increasingly unsustainable patterns of commerce, urban development, and modern life. With such an unprecedented mass migration and transformation, how can Chinese culture survive? What is to become of the existing million plus agricultural villages? How can these massively unsustainable new industrial towns survive? In the European Commission sponsored research program SUCCESS, researchers worked from the scale of the Chinese village to find viable answers to these questions.     

Publicly funded scientific entrepreneurs are not entitled to profit from their discoveries

Journal of Agricultural and Environmental Ethics -     

Community-based participatory research: Changing scientific practice from research on communities to research with and for communities

This paper examines community based participatory research from the viewpoint of the author, a doctoral student seeking to understand the concepts and framework of successful participatory research as a complement to traditional discipline driven research. Challenges and progress of CBPR within academia are examined, including acceptance, funding, training, and development of equal partnerships between university researchers and communities. Case studies (Latowsky, 2003; Ammerman et al., 2003; Stratford et al., 2003) focus on CBPR in different environmental justice communities, revealing the need to foster trust and communication in order to execute culturally competent and relevant research projects.     

Community-based participatory research: Changing scientific practice from research on communities to research with and for communities

The Agenda 21 treaty adopted at the 1992 United Nations Conference on Environment and Development set out new goals for the provision and management of information in a sustainable society. New information and communications technologies (ICTs) have the potential to be used as tools for managing information and consequently have a role in sustainable development. This paper considers the use of ICTs to manage local information areas and the extent to which these new technologies are compatible with the broader goals of Agenda 21 such as social inclusion and public participation in decision making. The use of the World Wide Web by local authorities in the UK to manage local information and promote Agenda 21 is explored through a critique of website contents. It is concluded that, although the use of ICTs is not incompatible with the broader aims of Agenda 21, and has many possible positive applications, current use of the World Wide Web by local government in the UK does not yet fulfil this potential.     

Diet modification to reduce phosphorus surpluses: a mass balance approach

Diet modification to reduce phosphorus (P) concentrations in manures has been developed in response to environmental concerns over P losses from animal agriculture to surface waters. We used USDA-NASS statistics on animal numbers and crop production to calculate county scale mass balances for manure P production, P removed in harvested portion of crops, and the potential effects of diet modification. Although spreading manure evenly over all crop acreage within a county is unlikely to occur, these calculations give a good indication as to the impact diet modification to reduce P can have at a regional or national scale. There was a high degree of regional variability in manure P surpluses (e.g., with the large crop acreages in the grain belt leading to large P offtake in crops preventing most P surpluses). In 89% of counties, there was a deficit of manure P relative to crop P removal; therefore there was a manure P surplus in 11% of counties. Diet modification decreased the percentage of states with a manure P surplus from 11 to 8%, a decrease of approximately 27%. Diet modification decreased the percentage of counties with the greatest surpluses of manure P (>30 kg ha(-1)) from 3% of all counties to 1%. Diet modification to decrease manure P is an important part of strategies to alleviate environmental concerns associated with surplus manure P in many areas, but additional strategies to deal with manure P surpluses are needed in some areas.     

Modelling visitor groups' intentions to displace from an urban trail: a combined stated preference and video monitoring approach

Depending on gender and activity, overcrowding and safety concerns may detract from urban green space use. Differences in use displacement intentions from a recreational trail in a forest in Vienna were investigated for male and female walkers as well as male and female dog-walkers (N = 425) using an image-based stated preference model. The trail scenarios were depicted as digitally calibrated images that systematically displayed combinations of levels of crowding with different mixes of user types and visitor behaviour. Visitors were asked whether the presented recreation scenario was so unacceptable that they would shift their use away from the presented trail. Hours and days of stated non-use of the recreation trail by various user groups are estimated by integrating the results of the behavioural model with annual visitation data. Use displacement intentions differed by gender and activity. High use levels were a greater concern for all respondents than very low use levels, particularly for female dog-walkers. However, only a small proportion of the recorded hours were heavily used. Safety concerns about very low use levels reduced the potential times of forest use for female walkers without a dog.     

Phosphorus desorption dynamics in soil and the link to a dynamic concept of bioavailability

Soils under intensive livestock farming and heavily fertilized with animal manure may have elevated soil phosphorus (P) contents. We determined P desorption kinetics in batch experiments using soils from a pot experiment where grass was cropped on a P-rich noncalcareous sandy soil without P addition, to lower the soil P content. A diffusion model was used to describe P desorption kinetics from a spherical aggregate. The model was calibrated with data from the batch experiments. Simulation results show that in the pot experiment, P desorption from the solid phase of the inner layers was initially far from equilibrium with the rest of the aggregate, but desorption came closer to equilibrium as the soil P content decreased further. A simple tool is presented, referred to as the dynamic bioavailability index (DBI), to determine whether kinetics of P desorption limits plant uptake. This tool is the dimensionless ratio of the modeled maximal diffusive flux from soil aggregates to solution and the plant uptake rate measured in the pot experiment. The DBI was initially much larger than one; the maximal possible P desorption rate exceeded the uptake rate, so uptake was not limited by desorption. The DBI stabilized at a value somewhat larger than one after a while, due to soil transport limitations. This decrease coincided with a large decrease of the P content in the grass to a value (far) below what is considered as optimal; the supply rate of P from soil to the root cannot meet the demand needed for optimal P uptake. The DBI could be seen as a promising onset to a new dynamic approach of bioavailability.     

Euro-trash: searching Europe for a more sustainable approach to waste management

How an economically affordable, environmentally effective and socially acceptable municipal solid waste management system can be developed is currently unclear. Considerable research has been carried out on the practical aspects of municipal waste management (i.e. transport, treatment and disposal) and how citizens feel about source separation, recycling, incineration and landfill but the perspective of the waste manager within the context of long term planning is often ignored. In this study, waste managers from 11 different leading-edge European municipal solid waste programs in nine different countries were interviewed. The economic, social, political, environmental, legal and technical factors of their specific programs were explored and analyzed. The transition of municipal solid waste management to urban resources management was observed and key ‘system drivers’ for more sustainable waste management practices were identified. Programs visited were: Brescia (I), Copenhagen (DK), Hampshire (UK), Helsinki (FI), Lahn-Dill-Kreis (D), Malmö (SE), Pamplona (E), Prato (I), Saarbrücken (D), Vienna (A), and Zürich (CH).     

Collaborative environmental governance and transaction costs in partnerships: evidence from a social network approach to water management in France

Several studies attempt to explain how collaborative environmental governance processes operate, but the question of why collaboration relationships form has received much less attention. Motivated by this need, this paper provides insights to the broad question: why does collaborative river basin management in France depend so heavily on partnerships made up around a few actors? Accordingly, our analytical framework develops a transaction cost explanation for the extent to which participatory procedures help stakeholders to identify partners and initiate collaboration, and for the causal link between the attributes of these stakeholders and their partnerships. The p2 model is implemented to investigate partnership networks of the key actors that govern the management of the Gironde estuary, the study case. The results provide evidence that environmental institutions bring together heterogeneous actors who might not be ready for collaboration, thereby actors’ perceived power similarity; their geographical proximity and co-presence in formal fora limit transaction costs.     

Urban ecological systems: scientific foundations and a decade of progress

Urban ecological studies, including focus on cities, suburbs, and exurbs, while having deep roots in the early to mid 20th century, have burgeoned in the last several decades. We use the state factor approach to highlight the role of important aspects of climate, substrate, organisms, relief, and time in differentiating urban from non-urban areas, and for determining heterogeneity within spatially extensive metropolitan areas. In addition to reviewing key findings relevant to each state factor, we note the emergence of tentative "urban syndromes" concerning soils, streams, wildlife and plants, and homogenization of certain ecosystem functions, such as soil organic carbon dynamics. We note the utility of the ecosystem approach, the human ecosystem framework, and watersheds as integrative tools to tie information about multiple state factors together. The organismal component of urban complexes includes the social organization of the human population, and we review key modes by which human populations within urban areas are differentiated, and how such differentiation affects environmentally relevant actions. Emerging syntheses in land change science and ecological urban design are also summarized. The multifaceted frameworks and the growing urban knowledge base do however identify some pressing research needs.     

Valuing wildlife: a review of methods

The paper analyses the costs and benefits of conserving wildlife and reports part of a wider research project which aims to estimate the benefits of certain ecological types and habitats, and to compare the outcome of different techniques used to value wildlife. The authors outline a range of philosophical, technical and methodological problems in deriving cardinal measures of costs and benefits.