Model microgravity enhances endothelium differentiation of mesenchymal stem cells

Mesenchymal stem cells (MSCs) are capable of differentiation into multilineage cell types under certain induction conditions. Previous studies have demonstrated that physical environments and mechanical force can influence MSC fate, indicating that these factors may be favorable inducers for clinical treatment. Our previous study found that MSCs are spread with a spindle shape when cultured in normal gravity (NG), and under modeled microgravity (MMG) for 72 h, they become unspread and round and their cytoskeleton fibers are reorganized. These morphological changes affected the function of MSCs through the activity of RhoA. We examined the responses of MSCs under MMG stimulation, followed with VEGF differentiation. We found that MSCs under MMG for 72 h were differentiated into endothelial-like cells by detecting the expression of endothelial-specific molecules (Flk-1 and vWF), which were also able to form a capillary network. Their endothelial differentiation potential was improved under MMG compared with that under NG. We believe that this method is a novel choice of MMG stimulation for neovascularization. This phenomenon may increase the potential of MSC differentiation, which might be a new strategy for the treatment of various vascular diseases and improve vascularization in tissue engineering.     

Neue Ergebnisse zur Linearität der Dosis/Wirkung-Beziehung strahleninduzierter Mutationen

Mutations induced by ionizing radiation in germ cells may affect future generations; mutations induced in somatic cells may damage the irradiated persons themselves, because radiation carcinogenesis is assumed to result from genetic damage induced in somatic cells. Since we are exposed mainly to low doses of ionizing radiation, both from natural and artificial sources, especially the dose dependence of radiation-induced mutations in the low-dose range is of interest. A review of recent studies on the induction of mutations by X-rays in human cells (in vitro) favors the hypothesis that in the low-dose range the dose dependence is linear, without a "threshold."     

Cell-free fetal DNA coming in all sizes and shapes

Cell-free fetal DNA analysis has an established role in prenatal assessments. It serves as a source of fetal genetic material that is accessible non-invasively from maternal blood. Through the years, evidence has accumulated to show that cell-free fetal DNA molecules are derived from placental tissues, are mainly of short DNA fragments and have rapid post-delivery clearance profiles. But questions regarding how they come to being short molecules from placental cells and in which physical forms do they exist remained largely unanswered until recently. We now know that the distributions of ending sites of cell-free DNA molecules are non-random across the genome and bear correlations with the chromatin structures of cells from which they have originated. Such an insight offers ways to deduce the tissue-of-origin of these molecules. Besides, the physical nature and sequence characteristics of the ends of each cell-free DNA molecule provide tell-tale signs of how the DNA fragmentation processes are orchestrated by nuclease enzymes. These realizations offered opportunities to develop methods for enriching cell-free fetal DNA to facilitate non-invasive prenatal diagnostics. Here we aimed to collate what is known about the biological and physical characteristics of cell-free fetal DNA into one article and explain the implications of these observations.     

Aggressive behavior of the male parent predicts brood sex ratio in a songbird

Brood sex ratio is often affected by parental or environmental quality, presumably in an adaptive manner that is the sex that confers higher fitness benefits to the mother is overproduced. So far, studies on the role of parental quality have focused on parental morphology and attractiveness. However, another aspect, the partner’s behavioral characteristics, may also be expected to play a role in brood sex ratio adjustment. To test this hypothesis, we investigated whether the proportion of sons in the brood is predicted by the level of territorial aggression displayed by the father, in the collared flycatcher (Ficedula albicollis). The proportion of sons in the brood was higher in early broods and increased with paternal tarsus length. When controlling for breeding date and body size, we found a higher proportion of sons in the brood of less aggressive fathers. Male nestlings are more sensitive to the rearing environment, and the behavior of courting males may often be used by females to assess their future parental activity. Therefore, adjusting brood sex ratio to the level of male aggression could be adaptive. Our results indicate that the behavior of the partner could indeed be a significant determinant in brood sex ratio adjustment, which should not be overlooked in future studies.     

Policy and technological constraints to implementation of greenhouse gas mitigation options in agriculture

A recent assessment of agricultural greenhouse gas (GHG) emissions has demonstrated significant potential for mitigation, but suggests that the full mitigation will not be realized due to significant barriers to implementation. In this paper, we explore the constraints and barriers to implementation important for GHG mitigation in agriculture. We also examine how climate and non-climate policy in different regions of the world has affected agricultural GHG emissions in the recent past, and how it may affect emissions and mitigation implementation in the future. We examine the links between mitigation and adaptation and drives for sustainable development and the potential for agricultural GHG mitigation in the future.We describe how some countries have initiated climate and non-climate policies believed to have direct effects or synergistic effects on mitigating GHG emissions from agriculture. Global sharing of innovative technologies for efficient use of land resources and agricultural chemicals, to eliminate poverty and malnutrition, will significantly mitigate GHG emissions from agriculture.Previous studies have shown that as less than 30% of the total biophysical potential for agricultural GHG mitigation might be achieved by 2030, due to price- and non-price-related barriers to implementation. The challenge for successful agricultural GHG mitigation will be to remove these barriers by implementing creative policies. Identifying policies that provide benefits for climate, as well as for aspects of economic, social and environmental sustainability, will be critical for ensuring that effective GHG mitigation options are widely implemented in the future.     

How to kick off system innovation: a Rotterdam case study of the transition to a fuel cell transport system

Fuel cells in combination with hydrogen are expected to play an important role in a future sustainable transport system. Recent studies have provided insight into how technological transitions may come about and may be managed. However, there is a lack of methodologies aimed at starting off transitions or system innovations, in practice. This paper addresses this issue by presenting a methodology characterized by a bottom–up approach to generate a roadmap for short-term projects within a long-term perspective. The methodology is applied in a Rotterdam case study of the transition to a fuel cell transport system. The outcome of this case study leads to recommendations for setting up a framework to facilitate and stimulate kicking off system innovation.     

Assessing potential future urban heat island patterns following climate scenarios,socio-economic developments and spatial planning strategies

Climate change and urban development will exacerbate current urban heat island effects. While most studies acknowledge the importance of projected temperature increases for raising urban temperatures, little attention is paid to the impacts of future changes in urbanisation patterns. Yet, steering urban development may be an effective strategy to further limit increases in the intensity and spreading of the urban heat island effect. We describe a method that allows exploring the impact of urban development scenarios on the urban heat island effect. This paper starts with a basic analysis of the strength of this effect in a temperate climate under relatively favourable conditions based on data from amateur weather stations and own observations. It explains local variation in observed temperatures and quantifies how the urban heat island effect may develop in the coming 30 years. Using the obtained relations, we assess potential future changes building on existing scenarios of climatic and socio-economic changes and a land use simulation model. Our measurements for the Amsterdam region in the Netherlands indicate that the urban heat island effect induces maximum temperature differences with the surrounding countryside of over 3 °C on moderately warm summer days. The simulations of potential future changes indicate that strong local temperature increases are likely due to urban development. Climate change will, on average, have a limited impact on these changes. Large impacts can, however, be expected from the combination of urban development and potentially more frequent occurrences of extreme climatic events such as heat waves. Spatial planning strategies that reduce the lateral spread of urban development will thus greatly help to limit a further increase in urban heat island values.     

Modelling economic impacts and adaptation to extreme events: Insights from European case studies

Adaptation to climate change in Europe has only recently become a true policy concern with the management of extreme events one priority item. Irrespective of future climatic changes increasing the need for systematic evaluation and management of extremes, weather-related disasters already today pose substantial burdens for households, businesses and governments. Research in the ADAM project identified substantial direct risks in terms of potential crop and asset losses due to combined drought and heatwave, as well as flood hazards in Southern and Eastern Europe, respectively. This paper focuses on the indirect, medium to longer term economic risks triggered by the direct risks and mediated by policy responses. We present a selection of three economic impact and adaptation assessments and modelling studies undertaken on extreme event adaptation in Europe. Responding to a need for more economically based adaptation assessments, we address some relatively unresearched issues such as the understanding of past adaptation, the role of market response to impacts as well as government’s ability to plan for and share out extreme event risks. The first analysis undertakes an empirical exploration of observed impacts and adaptation in the agricultural sector in the UK comparing the impact of consecutive extreme events over time in order to determine whether adaptation has occurred in the past and whether this can be used to inform future estimates of adaptation rates. We find that farmers and the agricultural sector clearly have adapted to extreme events over time, but whether this rate can be maintained into the future is unclear, as some autonomous adaptation enacted seemed rather easy to be taken. Markets may mediate or amplify impacts and in the second analysis, we use an economic general equilibrium model to assess the economic effects of a reduction in agricultural production due to drought and heatwave risk in exposed regions in Spain. The analysis suggests that modelled losses to the local economy are more serious in a large-scale scenario when neighbouring provinces are also affected by drought and heatwave events. This is due to the supply-side induced price increase leading to some passing on of disaster costs to consumers. The simulation highlights the importance of paying particular attention to the spatial and distributional effects weather extremes and possibly changes therein induced by climate change may incur. Finally, we discuss how national governments may better plan their disaster liabilities resulting from a need to manage relief and reconstruction activities post event. We do so using a risk based economic planning model assessing the fiscal consequences associated with the coping with natural extremes. We identify large weather-related disaster contingent liabilities, particularly in the key flood hot spot countries Austria, Romania, and Hungary. Such substantial disaster liabilities (“hidden disaster deficits”) when interacting with weak fiscal conditions may lead to substantial additional stress on government budgets and reduced fiscal space for funding other relevant public investment projects. Overall, our paper suggests the importance of respecting the specific spatial and temporal characteristics of extreme event risk when generating information on adaptation decisions. As our adaptation decisions considered, such as using sovereign risk financing instruments are associated with a rather short time horizon, the analysis largely focuses on the management of today’s extreme events and does not discuss in detail projections of risks into a future with climate change. Such projections raise important issues of uncertainty, which in some instances may actually render future projections non-robust, a constraint to be kept in mind when addressing longer term decisions, which at the same time should account for both climate and also socioeconomic change.     

To drink or grasp? How bullet ants (Paraponera clavata) differentiate between sugars and proteins in liquids

Flexibility in behavior can increase the likelihood that a forager may respond optimally in a fluctuating environment. Nevertheless, physiological or neuronal constraints may result in suboptimal responses to stimuli. We observed foraging workers of the giant tropical ant (also referred to as the “bullet ant”), Paraponera clavata, as they reacted to liquid solutions with varying concentrations of sugar and protein. We show that when protein/sucrose concentration is high, many bullet ants will often try to grasp at the droplet, rather than gather it by drinking. Because P. clavata actively hunt for prey, fixed action patterns and rapid responses to protein may be adaptively important, regardless of the medium in which it is presented. We conclude that, in P. clavata, food-handling decisions are made in response to the nutrient content of the food rather than the texture of the food. Further, we suggest that colonies that maintain a mixture of individuals with consistent fixed or flexible behavioral responses to food-handling decisions may be better adapted to fluctuating environmental conditions, and we propose future studies that could address this.     

Soil biodiversity for agricultural sustainability

We critically highlight some evidence for the importance of soil biodiversity to sustaining (agro-)ecosystem functioning and explore directions for future research. We first deal with resistance and resilience against abiotic disturbance and stress. There is evidence that soil biodiversity does confer stability to stress and disturbance, but the mechanism is not yet fully understood. It appears to depend on the kind of stress and disturbance and on the combination of stress and disturbance effects. Alternatively, community structure may play a role. Both possible explanations will guide further research. We then discuss biotic stress. There is evidence that soil microbial diversity confers protection against soil-borne disease, but crop and soil type and management also play a role. Their relative importance as well as the role of biodiversity in multitrophic interactions warrant further study. Henceforth, we focus on the effects of plant and soil biodiversity on nutrient and water use efficiencies as important ecological functions in agroecosystems. The available evidence suggests that mycorrhizal diversity positively contributes to nutrient and, possibly, water use efficiency. Soil fauna effects on nutrient and water use efficiencies are also apparent, but diversity effects may be indirect, through effects on soil structure. We present a conceptual diagram relating plant and soil biodiversity with soil structure and water and nutrient use efficiencies as a framework for future studies. We then consider how cropping systems design and management are interrelated and how management options might be interfaced with farmers’ knowledge in taking management decisions. Finally, we attempt to express some economic benefits of soil biodiversity to society as part of a wider strategy of conserving and using agrobiodiversity.     

Fetal tissue involvement in the late infantile type of neuronal ceroid lipofuscinosis

Prenatal diagnosis in a pregnancy at risk for late infantile neuronal ceroid lipofuscinosis (Batten's disease) was undertaken at 17 weeks' gestation by ultrastructural examination of amniotic fluid cells. The presence of curvilinear profiles indicated an affected fetus and the diagnosis was confirmed, after the pregnancy was terminated, by the finding of many typical curvilinear profiles in multiple tissues which included skin, amnion, umbilical vessels, blood, liver, and brain. Comparison between the involved cells in the amniotic fluid and fetal tissues suggests that these cells are probably derived from the periderm, and possibly also from the amnion. The prominent presence of cytosomes in the periderm and intermediate cells of the fetal epidermis and occasionally also in the endothelial cells of the dermis suggests that fetal skin may be a useful alternative site for assessing fetal involvement. Control specimens of the amniotic fluid, fetal skin, amnion, and liver showed no similar cytosomes. However, some control amniotic fluid samples did contain cells with large collections of irregular trilaminar membranes, and these could be open to misinterpretation. It is important that only typical curvilinear profiles are considered as an indication of an affected pregnancy.     

Syndromic associations with congenital anomalies of the fetal thorax and abdomen

Anomalies of the thorax and abdomen can be found in a number of genetic syndromes. Whilst it may not be possible to make a definitive diagnosis before birth, knowledge of the potential associations can be useful for the prenatal diagnostician when examining the fetus and counselling the parents. In this article, we describe conditions where other features may be detectable using prenatal ultrasound. We describe the features, potential diagnostic aids and prognosis. The tables list other potential features that may be identified. The range of conditions that can occur emphasises the value of genetic input in the management of a fetus with an apparently normal karyotype and multiple anomalies, the need to save material for future molecular analysis and the requirement of a detailed examination after delivery. These are needed in order to make accurate diagnoses and advise parents with regard to recurrence risks and the potential for prenatal diagnosis in future pregnancies. Copyright © 2008 John Wiley & Sons, Ltd.     

Plenty of water,not enough strategy: How inadequate accessibility,poor governance and a volatile government can tip the balance against ensuring water security: The case of Nepal

Water security is arguably the single most important factor regarding the future sustainability of our planet. Without water we have no life, and with depleting freshwater resources conflict can easily ensue. The intersection between hydrology and politics requires a delicate balancing from decision-makers to ensure policy is well-informed and science is well communicated. In this paper, we discuss water issues currently faced by Nepal, a nation where freshwater resources are abundant yet political pressures are threatening future water security. We argue that despite adequate water supplies a nation may still experience severe water insecurity, particularly if effective governance and equitable access are not prioritised. We explore potential policy options necessary to achieve a holistic framework for water resources management, which we suggest, need to consider water resource reliability, accessibility and governance as fundamental pillars for ensuring water security.     

Biomolecular rods and tubes in nanotechnology

Biomolecules are vitally important elements in nanoscale science and also in future nanotechnology. Their shape and their chemical and physical functionality can give them a big advantage over inorganic and organic substances. While this becomes most obvious in proteins and peptides, with their complicated, but easily controlled chemistry, other biomolecular substances such as DNA, lipids and carbohydrates can also be important. In this review, the emphasis is on one-dimensional molecules and on molecules that self-assemble into linear structures, and on their potential applications. An important aspect is that biomolecules can act as templates, i.e. their shape and chemical properties can be employed to arrange inorganic substances – such as metals or metal compounds – on the nanometre scale. In particular, rod- and tube-like nanostructures can show physical properties that are different from those of the bulk material, and thus these structures are likely to be a basis for new technology.     

Irregular brood patterns and worker reproduction in social wasps

The potential for reproductive conflict among colony members exists in all social insect societies. For example, queens and workers may be in conflict over the production of males within colonies. Kin selection theory predicts that in a colony headed by a multiply-mated queen, worker reproduction is prevented by worker policing in the form of differential oophagy. However, few studies have demonstrated that workers actually lay eggs within queenright colonies. The purpose of this study was to determine if workers laid male eggs within unmanipulated queen-right colonies of the polyandrous social wasps Vespula maculifrons and V. squamosa. We focused our analysis on an unusual brood pattern within colonies, multiple egg cells. We were primarily interested in determining if individuals reared in these irregular circumstances were queen or worker offspring. To address this question, we genotyped 318 eggs from eight V. maculifrons and two V. squamosa colonies. No worker‑reproduction was detected in any of the queenright colonies; all of the eggs found in multiple egg cells were consistent with being queen‑produced. However, the frequency of multiple egg cells differed among colonies, suggesting that queens vary in the frequency of errors they make when laying eggs within cells. Finally, we suggest that workers may not be laying eggs within queenright colonies and that worker reproduction may be controlled through mechanisms other than differential oophagy in polyandrous Vespula wasps.     

The potential and economics of bioenergy in Finland

This article summarises the results from four studies on the potential of bioenergy, and one study on the economic feasibility of distributed renewable energy production, in Ostrobothnia, Finland. The scope of the studies was to explore the degree of energy self-sufficiency that could be achieved, and the economic implications and benefits.The results suggest that a great majority of rural areas have the potential for being self-sufficient in their energy production through bioenergy alone, but other renewable energy sources (RES), including for example wind and solar power, will make an even greater contribution in the future. This applies to areas especially outside the largest population centres and most energy intensive industries. Indeed, in peripheral regions the renewable energy potential far exceeds the energy demand, and the spatial coverage of energy self-sufficiency is huge. Other European and global studies also show tremendous RES potential.The economics of small scale power generation units, here represented by biogas plants, are already feasible, despite the technology still being at an early developmental phase. Diffusion of these technologies depends on a number of factors, but single and separate units are emerging all the time. The adoption of RES based energy management systems will however be a long social process.The results give rise to an expectation for more RES based energy generation in Ostrobothnia and Finland and even in Europe and globally. In a time frame of 20–30 years, this development may result in structural changes across the whole energy sector. This comprehensive movement can only be understood and supported by multi- and cross-scientific approaches, and it will in future be a challenge for forthcoming research.     

Carotenoid-dependent coloration of male American kestrels predicts ability to reduce parasitic infections

The signaling function of sexually selected traits, such as carotenoid-dependent avian plumage coloration, has received a great deal of recent attention especially with respect to parasitism and immunocompetence. We argue that parasite-mediated models of sexual selection may have an implicit temporal component that many researchers have ignored. For example, previous studies have demonstrated that carotenoid-dependent traits can signal past parasite exposure, current levels of parasitism, or the ability of individuals to manage parasitic infections in the future. We examined repeated measures of carotenoid-dependent skin color and blood parasitism in American kestrels (Falco sparverius) to distinguish whether coloration might signal current parasitism or the potential to deal with infections in the future. We found no evidence that coloration was related to current levels of parasitism in either sex. However, coloration of males significantly predicted their response to parasitism; males with bright orange coloration during prelaying, when mate choice is occurring, were more likely than dull yellow males to reduce their levels of infection by the time incubation began. Coloration during prelaying may advertise a male’s health later in the breeding season. For kestrels, the ability to predict future health would be highly beneficial given the male’s role in providing food to his mate and offspring. Coloration of females was not a significant predictor of parasitism in the future, and we provide several possible explanations for this result.