Occurrence of DNA methylation in Daphnia magna and influence of multigeneration Cd exposure

Most of the research on the epigenetic phenomenon of DNA methylation has been performed with vertebrates and plants. Knowledge on DNA methylation in Daphnia magna, a key test organism in aquatic toxicology, is completely lacking. Through epigenetic inheritance, effects of transient chemical exposure could be transferred to non-exposed generations, which could have a major impact on ecological risk assessment procedures. In this study, we determined if CpG methylation occurs in D. magna and if this can be influenced by exposure to toxic substances. Homologs of human DNA methyltransferases DNMT1, DNMT2 and DNMT3A were found in the partially available D. magna genome. Using an optimized “Amplification of Intermethylated Sites (AIMS)” technique, two methylated fragments were discovered in D. magna DNA. No homology was found for these sequences. The methylation and the D. magna origin of the fragments were confirmed with Southern analysis. This optimized AIMS technique was then applied to DNA of D. magna which were exposed to 180 µg/L Cd for two generations. Exposure resulted in a significant decrease in reproduction. The same methylated fragments with the same band intensity were observed in DNA of both non-exposed and exposed daphnids. As such, it could not be demonstrated that Cd exposure altered DNA methylation. However, the presence of DNA methylation in D. magna shows that potentially epigenetic effects may occur in this species.     

Site Tenacity in the Willow Ptarmigan (<Emphasis Type="Italic">Lagopus lagopus</Emphasis> L.) in the Northern Yamal Peninsula

The return of willow ptarmigans to the sites in which they nested the previous year has been studied using color banding. No factors influencing the return index have been revealed, except for its dependence in males on the time when spring begins in a given year. In northernYamal, in contrast to the temperate zone, some surviving birds fail to return to their former nesting sites. Polymorphism of individuals with respect to territorial behavioral strategy is proposed for L. lagopus populations at the northern boundary of the species range.__________Translated from Ekologiya, No. 3, 2005, pp. 215–221.Original Russian Text Copyright © 2005 by Tarasov.     

Irregularity in frequency of mast seed years in <Emphasis Type="Italic">Quercus floribunda</Emphasis> a late successional species of Central Himalaya

The mid altitudinal oak, Quercus floribunda forms predominantly evergreen forest in Central Himalaya between 2000–2400 m. It is late successional, mature phase species that has limited regeneration on disturbance prone sites. This oak produces mast seed crops at an interval of 2–3 years. During masting in Q. floribunda the seed fall and germination is upto ten times greater than in normal years, emphasizing the importance of mast year crop in forest maintenance. However, no mast year in this species since the last nine years (1997–2005) is a matter of serious concern. The rise in the summer and winter temperature over a period of 15 years appear to have affected the frequency of masting in this oak. The importance of masting can be adjudged from the fact that 97% of the surviving seedlings m⁻² are of the mast year crop.     

Variations in breeding success and daily nest survival of Whiskered Tern (<Emphasis Type="Italic">Chlidonias hybrida</Emphasis>) at two Iranian colonies

Variations in breeding success of the Whiskered Terns (Chlidonias hybrida) were studied in two wetlands; Anzali (2005 and 2008) and Zarivar (2007–2008) in north and west Iran. Nesting success was modeled and compared using information-theoretic approach implemented in program Mark in order to assess effects of colony locations, years and nest initiation dates on the probability of daily nest survival. Clutch size did not vary between colonies and years. Breeding success varied between colonies (Zarivar > Anzali), and years only in Anzali (2005 > 2008). Overall daily nest survival was 0.977. (95% LCI 0.973, UCI 0.981), equivalent to a Mayfield nest survival of 0.317. (95% LCI 0.255, UCI 0.387). Egg-laying periods and colony site were important covariates affecting the daily survival rates (Σω _i = 0.86 and Σω _i = 0.83, respectively), while, the support for the effect of year was less (Σω _i = 0.41). We concluded that Lake Zarivar provides a more favorable nesting location for Whiskered Terns probably because of little changes in rainfall and water depth during the breeding seasons which influence breeding performance.     

Comparative Sensitivity to the Insecticides Deltamethrin and Esfenvalerate of Some Aquatic Insect Larvae (Ephemeroptera and Odonata) and Daphnia magna

Sensitivity to the pyrethroids deltamethrin and esfenvalerate (aqueous solution) and LC₅₀ have been determined in acute (96-h) toxicological tests on mayfly larvae (Cloeon dipterum and Caenis miliaria), damselfly larvae (Lestes sponsa and Cordulia aenea), and juveniles from a laboratory culture of Daphnia magna. Sensitivity to deltamethrin increases in the series C. aenea (Odonata) < D. magna (Cladocera) < L. sponsa (Odonata) < C. miliaria(Ephemeroptera) < C. dipterum (Ephemeroptera), and that to esfenvalerate, in the series C. aenea < D. magna < L. sponsa C. miliaria C. dipterum. The values of LC₅₀ about 0.01 g/l determined for mayfly larvae are below those known for various hydrobionts from the literature, indicating a very high sensitivity of these insects to pyrethroids.     

Exoautometabolic regulation of transgenes in microorganisms

Data on the existence of a specific system regulating the growth, size, and structure of enterobacterial populations are first summarized in terms of the chemical ecology concept formulated by S.S. Shvarts. The role of this system in transgene functioning is described using the example of the lux operon integrated into Escherichia coli. A relatively simple test for estimating such interactions is proposed.Translated from Ekologiya, No. 2, 2005, pp. 91–96.Original Russian Text Copyright © 2005 by Pshenichnov, Maslennikova, Nikitina.     

The Effect of Ambient Temperature on Intraspecific Interactions Controlling Heat Tolerance in the Oligochaetes Enchytraeus albidus and Tubifex tubifex

A signal (nonlethal) increase in ambient temperature (to 26 and 31°C for the oligochaetes Enchytraeus albidus and Tubifex tubifex, respectively) induces intraspecific interactions mediated by the release of pheromones into the medium. The pheromones cause physiological changes that persist after the cessation of either these interactions or pheromone action and are expressed as a considerable increase in the heat tolerance of individual E. albidus and T. tubifex.     

Phenogenetic monitoring of the weeping birch (<Emphasis Type="Italic">Betula pendula</Emphasis> Roth.) in the Middle Urals: testing a new method for assessing developmental instability in higher plants

Variation of bilateral leaf structures in Middle Ural cenopopulations of the weeping birch (Betula pendula Roth.) growing in gradients of industrial pollution was studied to test the efficiency of a new method of population and individual phenogenetic monitoring allowing an indirect assessment of developmental destabilization by segregating the variance of total asymmetry (TA _R ²) into its additive components, the variances of fluctuating asymmetry (FA _R ²) and directional asymmetry (DA _R ²). The method was tested in the impact zones of two copper-smelting plants in the Middle Urals. The degree of impact was characterized by the index of technogenic pollution (ITP) reflecting the average total contents of 15 water-soluble pollutants in snow samples. The level of asymmetry was estimated from the numbers of denticles with incoming veins (dentovenal elements) on the left and right leaf margins. Spearman’s coefficient of rank correlation between the group values of variance in fluctuating asymmetry (FA _R ²) and corresponding ITP values reached r _S = 0.914 (p < 0.001), providing evidence that the method is highly appropriate for ecological indication of the phenogenetic response of trees to environmental pollution.     

Bioindication-based Estimation of Technogenic Impact on <Emphasis Type="Italic">Pinus sylvestris</Emphasis> L. Populations in the Vicinity of a Radioactive Waste Storage Facility

The spectrum and frequency of cytogenetic aberrations in the reproductive (seeds) and vegetative (foliage) organs of Scotch pine (Pinus sylvestris L.) have been studied in the vicinity of the LSK Radon facility for radioactive waste storage and processing and in a 30-km zone around the Chernobyl Nuclear Power Plant. The results indicate that the pine populations of these regions are exposed to mutagenic factors. In contrast to the 30-km Chernobyl zone, the increased environmental mutagenicity in the vicinity of LSK Radon and in the center of the city of Sosnovyi Bor is mainly accounted for by chemical factors. The results of additional acute γ-irradiation have shown an increased radiation resistance of Scotch pine seeds from the LSK Radon and Sosnovyi Bor populations. Regression analysis demonstrated a significant increase in the cytogenetic aberration rate in plants from the experimental plots throughout the study period (1997–2002).__________Translated from Ekologiya, No. 4, 2005, pp. 275–285.Original Russian Text Copyright © 2005 by Geras’kin, Vasil’ev, Dikarev, Udalova, Evseeva, Dikareva, Zimin.     

Effects of acute γ-irradiation on the aquatic microbial microcosm in comparison with chemicals

Effects of acute γ-irradiation were investigated in the aquatic microcosm consisting of green algae (Chlorella sp. and Scenedesmus sp.) and a blue–green alga (Tolypothrix sp.) as producers; an oligochaete (Aeolosoma hemprichi), rotifers (Lecane sp. and Philodina sp.) and a ciliate protozoan (Cyclidium glaucoma) as consumers; and more than four species of bacteria as decomposers. At 100 Gy, populations were not affected in any taxa. At 500–5000 Gy, one or three taxa died out and populations of two or three taxa decreased over time, while that of Tolypothrix sp. increased. This Tolypothrix sp. increase was likely an indirect effect due to interspecies interactions. The principal response curve analysis revealed that the main trend of the effects was a dose-dependent population decrease. For a better understanding of radiation risks in aquatic microbial communities, effect doses of γ-rays compared with copper, herbicides and detergents were evaluated using the radiochemoecological conceptual model and the effect index for microcosm.     

Characteristics of Cajander Larch (<Emphasis Type="Italic">Larix cajanderi</Emphasis> Mayr) on Dredging Waste Dumps in the Amur Region

Former dredging sites were studied in the taiga zone of the Amur region (in Khabarovsk Krai). The involvement of Larix cajanderi in plant succession on dredging waste dumps was revealed. Some morphological-anatomical parameters of L. cajanderi needles were compared in trees growing on the dumps and in adjacent open larch stands on waterlogged peaty soils.__________Translated from Ekologiya, No. 4, 2005, pp. 259–263.Original Russian Text Copyright © 2005 by Osipov, Burundukova.     

Effects of the <Emphasis Type="Italic">Astacus astacus</Emphasis> L. Population on Biomass of Macrophytes in a Fresh Water Body

In experiments performed in aquariums, the daily consumption of Chara vulgaris alga by crayfish (Astacus astacus L.) has been determined. These quantitative data have been used to make a prognosis of the effect of the A. astacus population on the biomass of macrophytes in Lake Berezovo (Pskov oblast). The density of the crayfish population and the biomass of higher aquatic vegetation in the lake have been determined in field studies. Extrapolation of the results of laboratory experiments to a natural water body has shown that crayfish are capable of controlling no less than 40% of submersed macrophytes in the area used by their population.__________Translated from Ekologiya, No. 4, 2005, pp. 300–305.Original Russian Text Copyright © 2005 by Kholodkevich, Shumilova, Fedotov, Zhuravlev.     

On the Nexus of SO<Subscript>2</Subscript> and CO<Subscript>2</Subscript> emissions in China: the ancillary benefits of CO<Subscript>2</Subscript> emission reductions

This paper examines the issue of ancillary benefits by linking sulfur dioxide (SO₂) emission to CO₂ emission using a panel of 29 Chinese provinces over the period 1995–2007. In the presence of non-stationarity and cointegrating properties of these two data series, this paper applies the panel cointegration techniques to examine both the long-run and short-run elasticities of SO₂ with respect to CO₂. The major findings are that: (1) there exhibits a stable long-run equilibrium relationship between the SO₂ and CO₂ emission with the long-run elasticity being 2.15; (2) there exists a short-run relationship between these two emissions with the short-run elasticity being 0.04. In addition, following an exogenous shock that causes a deviation from the long-run equilibrium, it would take approximately 15 years for SO₂ emission to revert toward the long-run equilibrium path with an average annual convergence rate of 6.5%; (3) the derived ancillary benefits that is generated from one metric ton of CO₂ emission reduction, are 11.77 Yuan (approximately US1.7) in the short run and 196.16 Yuan (US 1.7) in the short run and 196.16 Yuan (US 30) in the long run. These findings are not only crucial from the econometric modeling perspective, but also have important policy implications.     

Estimation of the possibility of cladoceran invasion and survival under conditions of competition in mesotrophic Lake Glubokoe

A possibility of introducing alien cladoceran species into the pelagial zone of mesotrophic Lake Glubokoe has been estimated. For this purpose, observations have been performed on the cohorts of alien species (Daphnia magna and Simocephalus exspinosus) and aboriginal species (Daphnia hyalina and Diaphanosoma brachyurum). The results show that competition is a factor preventing the introduction of weak competitors, such as D. magna, but admitting stronger competitors, e.g., S. exspinosus. Competition in the lake may influence not only the species structure of the zooplanktonic community, but also its vertical distribution.     

Ecological and genetic characteristics of the distribution of <Emphasis Type="Italic">Bradybaena fruticum</Emphasis> Mull. (mollusca,gastropoda, pulmonata) in a forest-steppe landscape

The distribution pattern of the gastropod Bradybaena fruticum has been studied in a forest-steppe landscape. Analysis of the frequencies of polymorphic biochemical and conchological characters has yielded preliminary results on the characteristics of the species population structure whose formation was determined by a set of natural and anthropogenic factors.Translated from Ekologiya, No. 1, 2005, pp. 39–47.Original Russian Text Copyright © 2005 by Snegin.     

Relationship between morphological disparity and taxonomic diversity in rodent communities in the zone of influence from the Eastern Ural Radioactive Trace in the Southern Urals

To test the hypothesis that morphological disparity increases while taxonomic diversity decreases in a radioactively contaminated environment, the relationship of these parameters was studied in communities (taxocenes) of rodents living in the Southern Urals in the zone of influence from the Eastern Ural Radioactive Trace (EURT) (2003–2008). Young of the year of six rodent species (98 samples) were examined in the background (control) area (0.2 Ci/km²) and in the impact area polluted with radionuclides from the EURT (750 Ci/km²). The dynamics of morphological disparity (MD), assessed with regard to average values of five exterior characters, was compared between the control and impact taxocenes. Taxonomic (taxocenotic) diversity was estimated with the Shannon index (H). Morphological disparity was found to increase significantly at low rodent abundance (in conditionally unfavorable years) and decrease at high abundance, with the value of MD in the impact taxocene being significantly higher. A negative correlation between MD and H was revealed (r = −0.70), in conformity with the above hypothesis. Discordance (difference in direction) of annual changes in the test parameters, with H increasing while MD decreases (or vice versa) was observed in unfavorable years; it can be used as an indicator of conditions unfavorable for rodent taxocenes.     

Expansion of Evergreen Conifers to the Larch-Dominated Zone and Climatic Trends

The expansion of so-called evergreen conifers (EGCs), including Siberian stone pine, spruce, and fir, along the transect oriented from the boundary of the larch-dominated zone (LDZ; mixed forests of the Yenisei Ridge) to its center has been studied. The normalized dispersal coefficient calculated as K _i = (n _i – N _i )/(n _i + N _i ), where n _i and N _i are the relative numbers of the ith species in the undergrowth and the upper layer, respectively, serves as an indicator of the expansion. It has been found that the K _i values for EGCs (and birch) are higher than the K _i of larch even in the zone absolutely dominated by larch, where the relative numbers of EGCs in the upper layer is less than 1%. The EGC undergrowth has mainly been formed during the past 20–30 years, which is correlated with the trend of summer temperatures The spread of EGCs in the LDZ depends on the frequency of forest fires. The decrease in the time intervals between fires in the 20th century to 65 years (versus 100 years in the 19th century) may have prevented the expansion of competing species in the LDZ. The results obtained indicate that EGCs and birch penetrate into the zone traditionally dominated by larch, which is related to climatic changes during the past three decades. At the same time, tree stand density is increasing in the forest-tundra ecotone, and larch is spreading further into the tundra zone.__________Translated from Ekologiya, No. 3, 2005, pp. 186–193.Original Russian Text Copyright © 2005 by Kharuk, Dvinskaya, Ranson, Im.     

Intrapopulation Differentiation by the Rates of Rosette Growth and Individual Plant Development in Narrow-Leaved Hawk’s Beard (<Emphasis Type="Italic">Crepis tectorum</Emphasis> L.): Aftereffects of Long-Term Stress

Aftereffects of exposure to long-term chemical pollution with fluorine and sulfur compounds on intrapopulation differentiation of the rates of growth and development has been studied on Crepis tectorum L. progenies obtained from seeds collected in a pollution gradient and grown in a greenhouse under uniform ecological conditions. The results have shown that long-term stress (exposure to toxic gaseous pollutants) leads to significant changes in the intrapopulation structure, which are manifested in an increase in the proportion of plants with a low rate of development, a low relative rate of the linear growth of metameres (leaves), but a higher relative rate of their formation.__________Translated from Ekologiya, No. 4, 2005, pp. 243–251.Original Russian Text Copyright © 2005 by Trubina.     

Priority and emerging flame retardants in rivers: Occurrence in water and sediment,Daphnia magna toxicity and risk assessment

The occurrence, partitioning and risk of eight polybrominated diphenyl ethers (PBDEs), nine new brominated (NBFRs) and ten organophosphorus flame retardants (OPFRs) were evaluated in three Spanish rivers suffering different anthropogenic pressures (Nalón, Arga and Besòs). OPFRs were ubiquitous contaminants in water (ΣOPFRs ranging from 0.0076 to 7.2 μg L^− 1) and sediments (ΣOPFRs ranging 3.8 to 824 μg kg^− 1). Brominated flame retardants were not detected in waters, whereas ΣPBDEs ranged from 88 to 812 μg kg^− 1 and decabromodiphenyl ethane (DBDPE) reached 435 μg kg^− 1 in sediments from the River Besòs, the most impacted river. The occurrence of flame retardants in river water and sediment was clearly associated with human activities, since the highest levels occurred near urban and industrial zones and after wastewater treatment plants discharge. Daphnia magna toxicity was carried out for OPFRs, the most ubiquitous flame retardants, considering individual compounds and mixtures. Toxicity of nine tested OPFRs differed largely among compounds, with EC₅₀ values ranging over three magnitude orders (0.31–381 mg L^− 1). Results evidenced that these compounds act by non-polar narcosis, since their toxicity was proportional to their lipophilicity (K_ow). Furthermore, their joint toxicity was additive, which means that single and joint toxicity can be predicted knowing their concentration levels in water using quantitative structure activity relationships (QSARs) and predictive mixture models. Based on these results, a risk assessment considering joint effect was performed calculating and summing risk quotients (RQs) for the water and sediment samples. No significant risk to D. magna (ΣRQs < 1) was observed for any of the monitored rivers.     

Air pollution and diabetes association: Modification by type 2 diabetes genetic risk score

Exposure to ambient air pollution (AP) exposure has been linked to type 2 diabetes (T2D) risk. Evidence on the impact of T2D genetic variants on AP susceptibility is lacking. Compared to single variants, joint genetic variants contribute substantially to disease risk. We investigated the modification of AP and diabetes association by a genetic risk score (GRS) covering 63 T2D genes in 1524 first follow-up participants of the Swiss cohort study on air pollution and lung and heart diseases in adults. Genome-wide data and covariates were available from a nested asthma case-control study design. AP was estimated as 10-year mean residential particulate matter < 10 μm (PM₁₀). We computed count-GRS and weighted-GRS, and applied PM₁₀ interaction terms in mixed logistic regressions, on odds of diabetes. Analyses were stratified by pathways of diabetes pathology and by asthma status. Diabetes prevalence was 4.6% and mean exposure to PM₁₀ was 22 μg/m³. Odds of diabetes increased by 8% (95% confidence interval: 2, 14%) per T2D risk allele and by 35% (− 8, 97%) per 10 μg/m³ exposure to PM₁₀. We observed a positive interaction between PM₁₀ and count-GRS on diabetes [OR_interaction = 1.10 (1.01, 1.20)], associations being strongest among participants at the highest quartile of count-GRS [OR: 1.97 (1.00, 3.87)]. Stronger interactions were observed with variants of the GRS involved in insulin resistance [(OR_interaction = 1.22 (1.00, 1.50)] than with variants related to beta-cell function. Interactions with count-GRS were stronger among asthma cases. We observed similar results with weighted-GRS. Five single variants near GRB14, UBE2E2, PTPRD, VPS26A and KCNQ1 showed nominally significant interactions with PM₁₀ (P < 0.05). Our results suggest that genetic risk for T2D may modify susceptibility to air pollution through alterations in insulin sensitivity. These results need confirmation in diabetes cohort consortia.