The purpose of this work is to study the co-cultivation of Chlorella sp. and wastewater wild algae under different cultivation conditions (i.e. CO2, light intensity, cultivation time, and inoculation ratio) for enhanced algal biomass and lipid productivity in wastewater medium using Response Surface Methodology (RSM). The results show that mixed cultures of Chlorella sp. and wastewater wild algae increase biomass and lipid yield. Additionally, findings indicate that CO2, light intensity and cultivation time significantly affect algal productivity. Furthermore, CO2 concentration and light intensity, and CO2 concentration and algal composition, have an interactive effect on biomass productivity. Under different cultivation conditions, the response of algal biomass, cell count, and lipid productivity ranges from 2.5 to 10.2 mg/mL, 1.1 × 106 to 8.2 × 108 cells/mL, and 1.1 × 1012 to 6.8 × 1012 total fluorescent units/mL, respectively. The optimum conditions for simultaneous biomass and lipid accumulation are 3.6% of CO2 (v/v), 160 µmol/m2/s of light intensity, 1.6/2.4 of inoculation ratio (wastewater-algae/Chlorella), and 8.3 days of cultivation time. The optimal productivity is 9.8 (g/L) for dry biomass, 8.6 E + 08 (cells/mL) for cell count, and 6.8 E + 12 (Total FL units per mL) for lipid yield, achieving up to four times, eight times, and seven times higher productivity compared to nonoptimized conditions. Provided is a supportive methodology to improve mixed algal culture for bioenergy feedstock generation and to optimize cultivation conditions in complex wastewater environments. This work is an important step forward in the development of sustainable large-scale algae cultivation for cost-efficient generation of biofuel.
We investigated the effects of ethyl 2-methyl acetoacetate (EMA) on growth of the marine diatom algae Phaeodactylum tricornutum (P. tricornutum) and Skeletonema costatum (S. costatum). Growth of P. tricornutum was significantly inhibited by the minimum concentration (3.5 mmol·L?1) of EMA at lower initial algal densities (IADs) (3.6×104 and 3.3×105 cells·mL ?1). However, at the highest IAD, significant growth inhibition was found at above 7 mmol·L ?1 of EMA exposure. In S. costatum, EMA concentrations of 10.5 mmol·L ?1 or more significantly inhibited growth at lower IAD (3×104 and 1.8×105 cells·mL ?1); at the highest IAD, only EMA concentrations above 14 mmol·L ?1 obviously inhibited the growth of S. costatum. Changes in specific growth rates and pigment were consistent with algal growth, but only at higher EMA concentrations or lower IAD values was the ratio of chlorophyll a (Chla) to carotenoid significantly lower than the control. Medium effective concentration (EC 50) values were in the order 4.07, 8.03 and 12.27 mmol·L ?1 for P. tricornutum and 7.48, 11.92 and 17.22 mmol·L ?1 for S. costatum. All these results show that the effect of EMA on the growth of algae was species specific and mainly depended on IAD, which might be an important factor to influence algal growth. 相似文献
To provide information on reclamation of multi-heavy metal polluted soils with conception of phytostabilization, a field survey on the uptake and accumulation of potentially toxic elements such as antimony (Sb), arsenic (As), lead (Pb), cadmium (Cd), copper (Cu), and zinc (Zn) in colonized plant species around the world’s largest antimony mine area, China, was conducted. Samples including leaves and shoots (including roots and stems) of colonized plants as well as rhizospheric soils were collected from eight sampling zones in the studied area. The results showed that the contents of Cu, Zn, and Pb in rhizospheric soils below plants were comparable to the corresponding background values of Hunan province, otherwise Sb, Cd, and As contents were extremely high (17–106, 17–87, and 3–7 times of the corresponding background values). The highest concentration of Sb was found in Aster subulatus (410 mg kg?1); Cd, As, and Zn were in Herba bidentis bipinnatae (10.9, 264, and 265 mg kg?1, respectively); and Cu was in Artemisia lavandulaefolia (27.1 mg kg?1). It also exhibited that all the contents of As in leaves were several times of those in shoots of plants, Cd and other heavy metals showed in a similar pattern in several studied species, implying that the uptake route of these heavy metals via foliar might contribute to the accumulation. With high bioconcentration factors of heavy metals (more than 1, except for Zn), together with the growth abundance, Herba bidentis bipinnatae was considered as the most suitable colonized species for phytostabilization of the multi-heavy metal pollution in soils on this antimony mine area. 相似文献
This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 104 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost. 相似文献
When symbiotic dinoflagellate algae (Symbiodinium sp., isolated from the coral Plesiastrea versipora) were incubated with NaH14CO3 in the light in seawater, they released 22.69±9.16 nmol carbon/106 algae. Release of photosynthetically fixed carbon was stimulated more than six-fold for algae incubated in host-tissue homogenate
(148.54±97.03 nmol C/106 algae) and more than four-fold (102.00±49.16 nmol C/106 algae) for algae incubated in a low molecular weight fraction (≤1 000 Mr) prepared from host homogenate. Soluble released 14C-labelled products, as determined by chromatography and autoradiography, were the same when algae were incubated in either
host homogenate or the low molecular weight fraction. After 4 h incubation in the light (300 mol photons m−2 s−1),␣intracellular␣glycerol increased in algae incubated with the low molecular weight fraction (an increase of 0.39 to␣0.67 nmol glycerol/106 algae) compared with little or no increase in algae incubated in seawater (0 to 0.12 nmol glycerol/106 algae). Partial inhibition of triglyceride synthesis (up to 51%) was also observed when algae were incubated in the low molecular
weight fraction. All these effects are the same as those observed when algae were incubated in host homogenate. These data
indicate that the “host release-factor” activity of P.␣versipora is a compound of low molecular weight.
Received: 13 February 1997 / Accepted: 24 October 1997 相似文献
Iodine-129:iodine-127 ratios were determined using accelerator mass spectrometry in 34 Arctic marine algae collected between
1930 and 1993. A smaller set (5) of marine algae were also analyzed mass spectrometrically to determine plutonium-isotope
ratios. The 129I:127I ratio increased as much as three orders of magnitude from a mean of <1×10−11 (atom/atom) in the pre-nuclear era (before 1945) to nearly 1000×10−11 in 1993 for marine algae collected from the Novaya Zemlya archipelago separating the Barents and Kara Seas. The predominant
basis for the higher ratios in the Novaya Zemlya kelps appears to be upcurrent sources of 129I from nuclear fuel-reprocessing facilities at Sellafield (UK) and La Hague (France). Relatively high 241Pu:239Pu ratios (compared to observed bomb fallout at boreal latitudes, decay corrected to the date of collection) also corroborate
the influence of non-fallout sources. The small size of the data set precludes determining if there are significant contributions
of 129I and other radionuclides from Russian sources. In contrast, marine algae collected between 1969 and 1993 in the Bering, Beaufort
and East Siberian Seas had much lower 129I:127I ratios (mean=14.04×10−11±3.15 SD) than those observed in the European Arctic. The narrow range of ratios in Ameriasian Arctic kelps, and the modest
change over that time period, indicate that there were no major contributions of non-fallout 129I to North American Arctic surface waters at the time that the algae were collected. The potential for timing the transport
of fuel-reprocessed 129I through analysis of additional archived samples is outlined.
Received: 10 November 1997 / Accepted: 24 February 1998 相似文献
Xijiang River is an important drinking water source in Guangxi Province, China. Along the Xijiang River and surrounding tributary, the pollution profile of three important groups of semi-volatile organic compounds, including polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs) and phthalate esters (PAEs), was analyzed. Relatively low levels of PAHs (64–3.7 × 102 ng L?1) and OCPs (16–70 ng L?1), but high levels of PAEs (7.9 × 102–6.8 × 103 ng L?1) occurred in the water. Comparatively, low levels of OCPs (39–1.8 × 102 ng g?1) and PAEs (21–81 ng g?1), but high levels of PAHs (41–1.1 × 103 ng g?1) were found in sediment. Principal component analyses for source identification indicated petroleum-derived residues or coal and biomass combustion, and vehicular emission was the main sources for PAHs. The OCPs sources of each category were almost independent, whereas the new input of HCHs and p,p′-DDTs probably existed in some areas. PAEs were mainly originated from personal care products of urban sewage, plastic and other industrial sources. Ecological risk through the risk quotient analysis indicated a small or significant potential adverse effect on fish, daphnia and green algae. Nevertheless, the integrated risk of all pollutants should be taken into account in future study. 相似文献
Because iron is not available generally in oxygenated sea water, it may be a limiting factor in marine primary production. This hypothesis was tested in the context of Davies Reef, Latitude 18°50′S (one of the coral reefs in the central region of the Great Barrier Reef system). Samples were collected for study in the period August, 1980 to March, 1981. Sea water around the reef contained ≦2x10-6M Fe, surface sediments from the reef contained 66±26 (1 SD) ppm total Fe, and interstitial water near the surface contained ≧5x10-7M Fe. Thus, Fe constituted a trace component of the reef environment, but limited Fe should be available to algae associated with the sediments. Specific biochemical analyses to test the Fe status of benthic photosynthetic organisms were carried out with a common blue-green alga, Phormidium sp., and a ubiquitous symbiotic dinoflagellate, Gymnodinium microadriaticum (zooxanthellae). The blue-green alga contained the electron transport protein, flavodoxin, which is found only in Fe-deficient organisms. Supporting evidence for Fe stress in this organism included chlorosis in the presence of plentiful biliprotein, and very low extractable photosynthetic cytochrome, c-553. The latter observations were shown to be the result of Fe deficiency in laboratory cultures of a blue-green alga, Synechococcus sp. These cultures showed that production of flavodoxin is not a universal response of algae to Fe stress, but that lowered cellular concentrations of Fe-containing proteins involved in photosynthesis probably is universal. The zooxanthellae from a soft coral, Sinularia sp., had three-fold lower total Fe and ferredoxin (an electron transport protein), than the same alga from a clam, Tridacna maxima. Thus, some algae in symbiotic associations may also suffer Fe-deficiency. It was concluded that the degree and extent of Fe-stress in primary producers on a coral reef may influence growth rates, biomass, and distribution of species. 相似文献
The capability of several macroalgal species to protect photosynthesis from excessive irradiance by dynamic photoinhibition was investigated relative to their depth distribution in summer 1995 in the Kongsfjord (79°N; 12°E, Ny Ålesund, Spitsbergen, Norway). Photoinhibition of photosynthesis was induced by exposure of algae from different water depths to a high photon fluence rate of 500?μmol?m?2?s?1 for 2?h. Changes in optimal quantum yield (Fv/Fm) were measured during the inhibition phase. Recovery of photosynthesis was subsequently induced by dim white light (10?μmol?m?2?s?1) and observed as changes in the variable fluorescence. With a newly developed mathematical model different parameters of the response kinetics of inhibition and recovery were calculated and related to the depth distribution of each algal species. It is shown that two components with slow and fast reaction kinetics, respectively, are involved in photoinhibition and recovery of photosynthesis. Their possible molecular bases are discussed. The half-life time (τ) of the inhibition and recovery phases, i.e. the time necessary to reach half maximal response, is clearly related to the depth distribution of the investigated species. Algae collected close to the water surface show a fast reaction of both photoinhibition and recovery and, hence, have a low τ. With increasing depth the reactions become slower and τ increases. τ was highest in deep water algae. Further analysis of the reaction kinetics in Laminaria saccharina shows that the relative proportion of the two kinetics involved change with the collection depth. In contrast, a significant difference in the reaction rates of both kinetics was not observed. 相似文献
Paracentrotus lividus (Lamarck) larvae were reared to metamorphosis. The larvae were fed on the haptophycean Hymenomonas elongata Droop (Braarud) at three concentrations: 9 to 14×105, 24 to 37×105, 43 to 61×105 μm3 cells ml-1 d-1. Optimum growth took place at a density of 24 to 37×105 μm3 algal cells. Growth of the plutei was estimated in terms of weight increases in protein, carbohydrate and lipid; growth equations are given. The relationship between growth and the food ingested was calculated for the different larval stages. Earlier field data for the bay of Villefranche have shown the mean biovolume of nanoflagellates to be 0.65×105 μm3 ml-1; at such in situ food concentrations, P. lividus larvae would metamorphose only after one month of planktotrophic life. Chemoreception by larvae could lead to prey selection, thereby altering the amounts of protein, carbohydrate and lipid ingested, and hence the duration of larval life. 相似文献
Effects of copper sulphate on a marine food chain were investigated in large tanks at Loch Ewe on the west coast of Scotland. The food chain consisted of phytoplankton, the bivalve Tellina tenuis da Costa, and O-group plaice (Pleuronectes platessa L). During initial months after settlement, T. tenuis siphons are an important food for juvenile plaice, which feed by grazing the siphon tips; these subsequently regenerate. Copper dose rates of 10, 30 and 100 μg Cu/1 were investigated; the distribution of added copper was monitored and its metabolic effects were determined. Copper levels in water, sand, in algae on tank walls, in T. tenuis shell and flesh, and in plaice muscle and viscera were measured by atomic absorption spectrophotometry. Copper accumulated in sand, T. tenuis flesh and shell, and plaice viscera. Accumulations were dose-dependent and in no case was a plateau concentration reached. Most of the added dose was taken up by the sand; accumulations in T. tenuis shell were small — less than twice background in all cases. High levels were found in T. tenuis flesh, where concentrations after 100 days were 270, 470 and 1100 μg Cu/g dry flesh for dose concentrations of 10, 30 and 100 μg Cu/1, respectively. The mean control concentration was less than 50 μg Cu/g dry flesh. There was no accumulation of copper in plaice muscle. Visceral accumulations after 100 days were 71, 147 and 567 μg Cu/g dry flesh for dose concentrations of 10, 30 and 100 μg Cu/1, respectively. The mean control level was 30 μg Cu/g dry flesh. The effect of copper on phytoplankton metabolism was investigated by measuring plant pigment levels and rates of primary photosynthetic fixation of C14-labelled bicarbonate. All dose concentrations reduced both the standing crop and the rate of photosynthesis per unit of chlorophyll a. The effects of copper on growth and condition of T. tenuis and P. platessa were investigated. All dose concentrations adversely affected T. tenuis condition (dry flesh weight for standard individual). The effect was most marked during deposition of winter reserves. In the absence of plaice predation, there was a decrease in the mean siphon weight for T. tenuis exposed to 30 and 100 μg Cu/1. For plaice, all dose concentrations of copper resulted in reduced growth, but there was no significant change in condition or biochemical composition. The ash weight of fish exposed to copper was significantly higher than for controls. 相似文献
Net photosynthetic O2 evolution by five marine macroalgae:Ulva lactuca L.,Enteromorpha sp.,Ceramium strictum Harvey,Fucus serratus L., andF. vesiculosus L., collected from Danish waters in the summer of 1983 was followed at increasing O2 and with pH either fixed close to pH 7, 8 or 9, or drifting upwards during photosynthesis in a closed chamber to determine the effects of changing O2, pH and DIC (dissolved inorganic carbon) on photosynthesis. Increasing O2, increasing pH and decreasing DIC together limited O2 evolution. Raising the O2 concentration with pH and DIC held constant resulted in less inhibition of net-O2 evolution than when all three factors acted together. The O2 inhibition of photosynthesis was similar to the reported O2 inhibition of ribulose 1,5-bisphosphate carboxylase isolated from lower and higher plants. Net-O2 evolution as a function of the molar ratio of O2 to HCO–3 + CO2 in solution provided a general, linear relationship (r2 = 0.72 to 0.84), predicting inhibition of photosynthesis based on O2 pH and DIC changing together. Slopes of this relationship, representing competition between O2 and carbon based on external concentrations, were similar for the five taxonomically different algae, suggesting that similar processes act to reduce net-O2 evolution. 相似文献
Roxithromycin, as widely used medicine and livestock growth promoter, arouses concern because its occurrence and persistence in soil environments. However, effects of roxithromycin in higher plants are still vague. Accordingly, we hypothesized that roxithromycin-contaminated soil may exhibits ecotoxicological effects in wheat (Triticum aestivum). In this study, effects induced by a gradient concentration of roxithromycin stress (0.01, 0.1, 1, 10, and 100 mg·kg–1) was investigated in a 7-d soil test in T. aestivum. Results indicated that the specific activity of cytochrome P450 (CYP450) monooxygenase was decreased dramatically with the concentration of roxithromycin in soil. The IC50 value was 8.78 mg·kg–1 of roxithromycin. On the contrary, the growth related endpoints (i.e., the germination percentage, the biomass and the height), the content related endpoints (i.e., soluble protein content and CYP450 content), and the superoxide dismutase (SOD) activity failed to reveal the roxithromycin-induced effects. Further analysis revealed that the CYP450 monooxygenase specific activity reduction was enzymatic mechanism mediated, other than oxidative stress induced. We conclude that the soil roxithromycin declined the CYP450 monooxygenase activity in T. aestivum by the inhibition of the enzymatic mechanism. Further efforts can include, but are not limited to, investigation of joint effects induced by combined exposure of roxithromycin and the pesticides and evaluation of the similar effects in other higher plants. 相似文献
Attachment strength and colonization patterns of one barnacle (Balanus c.f. variegatus) and one polychaete species (Pomatoleios kraussii) on seven artificial substrata with surface tensions between 19 and 64.5 mNm-1 were studied between June 1991 and January 1992 at Laem Than (Chonburi province, Gulf of Thailand). The purpose of the present study was to demonstrate the role that surface tension plays under natural conditions in colonization success by these species. Does stronger adhesion to one substratum result in higher densities of specimens on that particular surface? Although both species adhere much better on substrata with higher surface tension than on surfaces with low surface tension (B. c.f. variegatus: between 0.04×105 and 16.35×105Nm-2 on surfaces of 22 and 33.5 mNm-1, respectively; P. kraussii: between 0.22×105 and 1.73×105Nm-2 on 19 and 33.5 mNm-1, respectively), colonization pattern is not influenced by surface tension. The major factors influencing settlement patterns seem to be space competition for B. c.f. variegatus and attraction to already settled adults by P. kraussii. The results show that the importance of surface tension in marine fouling control is limited because organisms colonize substrata successfully despite lower adhesion. Attachment strength is not a limiting factor under natural conditions. 相似文献
Cells of the coccolithophoridCricosphaera elongata (Droop) Braarud (Prymnesiophyceae) were concentrated by means of tangential ultrafiltration. Ultrastructural features of control cells were typical of the genusCricosphaera. The flagellar apparatus consists of two flagella, a bulbous haptonema, an axosome and a basal helical band, as recently reported in the literature for another coccolithophorid. We observed a very-well developed Golgi apparatus, displaying all the features of the peculiar Golgi of the Prymnesiophytes. Observations with the light microscope did not reveal any difference between control algae and algae contaminated with 1, 10 and 20 µmol Cd 1–1 (i.e., 112 µg 1–1, 1.12 and 2.24 mg Cd 1–1) for 48 h. However, algae treated with the highest cadmium concentration exhibited loss of motility after 6 h contact, but without apparent ultrastructural damage. Qualitative X-ray microanalysis of the dense granules present in Golgi vacuoles of control cells demonstrated the presence of calcium and chlorine and, at a lower level, sulphur and aluminium. The same elements were also identified in the dense part of the lysosomes. In situ,C. elongata produces coccoliths; this did not occur in culture. However, the coccolith-generating apparatus (Golgi complex and Ca2+) was present in culture, and the cells were able to synthesize unmineralized scales. 相似文献
Organophosphate, dichlorvos (2,2-dichlorovinyldimethylphosphate, DDVP), and diazinon (DZN) as well as carbamates are currently found in contaminated water, dust, soil, agricultural waste, and elsewhere in the environment, and can be harmful after accidental or deliberate exposure. Intoxication by these compounds causes a generalized cholinergic crisis due to the inhibition of acetylcholinesterase (AChE), whose major physiological role in mammalian tissues is in nervous transmission. The half-maximal inhibitory concentration (IC50) was found to be higher for carbaryl than DDVP and DZN, about 3–4 times in liver and about 4–6 times in muscles. The half time (t1/2) of inhibition (8?µmol?L?1 DDVP or DZN; 40?µmol?L?1 carbaryl) ranged between 4 and 16?min and decreased as follows: pig?>?cattle?>?sheep for liver tissue, and cattle?>?sheep?>?pig for muscle. Rate constants of inhibition (ki) ranged between 43?×?10?3 and 168?×?10?3?min?1 for liver and from 46?×?10?3 to 115?×?10?3?min?1 for muscles. Very little residual AChE activity (<6.1%) was seen in liver, but more was seen in muscle (<17%). 相似文献