Determination of Mancozeb in air. Occupational exposure during its use in control of potato late blight

A simple method for the determination of occupational exposure to Mancozeb (an ethylenebisdithio-carbamate) was evaluated. This method is based on filter sampling and determination of the manganese content in the samples by means of atomic absorption spectroscopy. The method shows good linearity and accuracy for determinations in the range 0.05 to 2 mg/m³. Exposure during field application of Mancozeb in potato cultivations was studied and was found, during preparation and filling of the spraying liquid, to be 0.22 mg/m³ on average (n=18). The highest individual eight-hour time-weighted average exposure was 0.05 mg/m³.     

Potential exposure of children and adults to cypermethrin following use of indoor insecticide foggers

The magnitude and distribution of cypermethrin from total release, over-the-counter foggers was studied in a test room and in residences to facilitate evaluation of regulatory exposure algorithms and new human exposure assessments based upon urine biomonitoring. Surface residue (SR) was evenly distributed in a small test room (3.6 μ g cypermethrin/cm²) where thorough mixing of the aerosol occurred. In a residence SR was significantly affected by room size and distance from the fogger. Air levels in the residence were as high as 30 μ g cypermethrin/cm³ after 4.5 h. The availability of surface residues was measured with an automated surface cotton cloth wipe and ethyl acetate extraction. Only 5% of the SR was available from nylon carpet. Tile, wood and linoleum resulted in 30, 10, and 10% of SR being available, respectively. These data are used to estimate cypermethrin exposure of children and adults for comparison with existing regulatory reference dosages and exposure assessments based upon biomonitoring.     

A comparison of the effects of organophosphate insecticide exposure and temperature stress on fluctuating asymmetry and life history traits in Culex quinquefasciatus.

Effects of exposure to a non-chemical (temperature) or chemical (organophosphate insecticide) stressor during larval development were compared in Culex quinquefasciatus mosquitoes. Stress was measured in two ways: using conventional life history traits (survival, development time and a measure of body size) and by calculating the degree of developmental instability from the departure from bilateral symmetry of wing characters (fluctuating asymmetry). Increasing insecticide dose, but not temperature, was observed to elevate wing fluctuating asymmetry in male but not female mosquitoes. Insecticide treatment reduced survival and was associated with a significant reduction in wing trait sizes in both females and males but did not significantly affect development time. Temperature was associated with a significant reduction in all life history traits in both sexes. Therefore wing fluctuating asymmetry in C. quinquefasciatus cannot be used as a general biomonitor of all stress, although it may have potential as a more specific monitor of chemical stress. It needs to be complimented with other measures such as life history and biochemical methods. The significant differences in response between sexes may impact on results of short-term larval exposures to insecticides.     

Titanium dioxide nanoparticles relieve biochemical dysfunctions of fifth-instar larvae of silkworms following exposure to phoxim insecticide

Phoxim insecticide is widely used in agriculture, which is toxic to insect pests and nontarget organisms. The phoxim poisoning is hard to prevent for silkworms. TiO₂ NPs have been widely applied in whitening, brightening foods, toothpaste or sunscreens, and orally-administered drugs. However, whether TiO₂ NPs can increase resistance of silkworm to phoxim poisoning has not been reported. The results demonstrated that added TiO₂ NPs significantly decreased reduction of protein, glucose and pyruvate contents, lactate dehydrogenase, succinate dehydrogenase and malate dehydrogenase activities, and attenuated increases of free amino acids, urea, uric acid and lactate levels, activities of protease, alanine aminotransferase and aspartate aminotransferase in the hemolymph of silkworms caused by phoxim exposure. From the present study, it is clearly evident that added TiO₂ NPs may relieve toxic impacts of phoxim insecticide on silkworm metabolism, which in turn may result in an increase in silk yield.     

Soil dissipation of juvenile hormone analog insecticide pyriproxyfen and its effect on the bacterial community

This investigation was undertaken to examine the dissipation rate of pyriproxyfen as well as the change in the soil bacterial community. Residues of pyriproxyfen were measured using high performance liquid chromatography (HPLC) and the changes in bacterial community were determined by comparing the 16S rDNA bands on patterns by denaturing gradient gel electrophoresis (DGGE). The dissipation of pyriproxyfen was affected by both the concentration applied and incubation temperature. Lower concentrations (1 mg Kg(-1)) and higher incubation temperatures (30 and 40°C) showed more rapid dissipation rates. The population of microbial community decreased rapidly after incubation with 10 mg Kg(-1) of pyriproxyfen for 91 days, indicating the toxicity of pyriproxyfen toward bacterial communities in a closed soil ecosystem. Lower concentrations of pyriproxyfen showed less toxicity toward the microbial community. From cluster analysis, the structure of the bacterial community showed roughly a 60 % similarity throughout the experiment period in the control experiment, indicating the stability within soil microbiota without chemical agitation. However, the similarity was lower than 50 % both in the one and 10 mg Kg(-1) of insecticide pyriproxyfen spiked experiment, indicating the soil bacterial community changed after the insecticide pyriproxyfen was applied.     

Effects of lindane on Daphnia magna during chronic exposure

Abstract

Acute and chronic toxicity tests with lindane were conducted on Daphnia magna. The 24‐hr static LC50 was 1.64 mgL^‐1. The sublethal effects of 0.16, 0.25, 0.32, 0.60 and 0.80 mgL^‐1 lindane on the survival, reproduction and growth of D. magna were monitored for 21 days. The algae Nannochloris oculata (5 × 10⁵ cellsmL^‐1) was used to feed the daphnids. The parameters used to determined the effect of the pesticide on D. magna were:mean total young per female, mean brood size, days to first brood, intrinsic rate of natural increase (r), growth, and survival. Reproduction as well as survival was significantly reduced at lindane concentrations of 0.25 mgL^‐1 and higher. The intrinsic rate of natural increase (r) decreased with increasing concentrations of lindane. Growth, as measured by body length, was depressed significantly at 0.25 mgL^‐1 lindane and higher. The chronic data was used to formulate an acute/chronic ratio.     

Translocation of aged cyclodiene insecticide residues from soil into forage crops and pastures at various growth stages under field conditions.

Field trials were conducted to measure translocation of pesticides by summer and winter forage/pasture species from soil containing aged residues of heptachlor and, to a lesser extent, dieldrin. Substantial amounts of heptachlor epoxide, and lesser amounts of gamma-chlordane were translocated to plants from contaminated soil. Residue levels varied with crop species and stage of plant development. In summer crops residues were higher in soybean > cowpeas > lab-lab > Sorghum > millet > sweet saccaline at the grazing and mature stages. Compared to glasshouse studies undertaken previously, residues in crops grown under field conditions were much lower. This apparently reflects the lower soil moisture levels and the reduced rates of translocation. Heptachlor residues in winter crops were highest in Saia oats > Berseem clover > Haifa clover > Cassia oats > Tetila ryegrass > Schooner barley > Shaftal clover > Hunter river lucerne at the grazing stage. There were no detectable levels in barley and oats at the mature stage. No dieldrin residues were translocated into the various crop species.     

A simple empirical model to predict forest insecticide ground-level deposition from a compendium of field data

Deposit data from 205 aerial forest insecticide applications conducted in field trials by the Canadian Forest Service, Great Lakes Forestry Centre over a 15-year period are summarized. Deposit measurements were taken under “worst case” scenarios in the sense that direct applications were made over water bodies, and ground samplers were intentionally placed in open or cleared areas of forest. The median % deposit on shoreline collectors (32 separate applications) was 5.7%, on mid-stream collectors (44 separate applications) was 6.2%, and on forest floor collectors (129 separate applications) was 4.9%. Forest floor deposit was most closely associated with application rate and droplet size (r = 0.624, p < 0.001 and r = 0.662, p = 0.011, respectively) but these variables combined only explained 44% of the variation in deposit. Data from all three collector types were grouped by 10% deposit increments and combined to provide a data set from all deposition scenarios. A negative exponential model was fitted to the proportion of these combined sites regressed on % deposit in 10% increments and plotted as a deposit probability distribution curve (p < 0.001, r² = 0.992). The probability distribution curve indicated that 5–10% deposit would be expected about 57–91% of the time, whereas 50% deposit or greater would be expected about 2% of the time or less. In a probabilistic risk assessment for aerially applied insecticides in a conifer-dominated forest environment, the probability distribution curve based on empirical data presented here can be used to refine the characterization of exposure scenarios from which effects estimates can be derived.     

A simple empirical model to predict forest insecticide ground-level deposition from a compendium of field data

Deposit data from 205 aerial forest insecticide applications conducted in field trials by the Canadian Forest Service, Great Lakes Forestry Centre over a 15-year period are summarized. Deposit measurements were taken under "worst case" scenarios in the sense that direct applications were made over water bodies, and ground samplers were intentionally placed in open or cleared areas of forest. The median % deposit on shoreline collectors (32 separate applications) was 5.7%, on mid-stream collectors (44 separate applications) was 6.2%, and on forest floor collectors (129 separate applications) was 4.9%. Forest floor deposit was most closely associated with application rate and droplet size (r = 0.624, p < 0.001 and r = 0.662, p = 0.011, respectively) but these variables combined only explained 44% of the variation in deposit. Data from all three collector types were grouped by 10% deposit increments and combined to provide a data set from all deposition scenarios. A negative exponential model was fitted to the proportion of these combined sites regressed on % deposit in 10% increments and plotted as a deposit probability distribution curve (p < 0.001, r2 = 0.992). The probability distribution curve indicated that 5-10% deposit would be expected about 57-91% of the time, whereas 50% deposit or greater would be expected about 2% of the time or less. In a probabilistic risk assessment for aerially applied insecticides in a conifer-dominated forest environment, the probability distribution curve based on empirical data presented here can be used to refine the characterization of exposure scenarios from which effects estimates can be derived.     

Impact of smoking on in-vehicle fine particle exposure during driving

Indoor smoking ban in public places can reduce secondhand smoke (SHS) exposure. However, smoking in cars and homes has continued. The purpose of this study was to assess particulate matter less than 2.5 μm (PM_2.5) concentration in moving cars with different window opening conditions. The PM_2.5 level was measured by an aerosol spectrometer inside and outside moving cars simultaneously, along with ultrafine particle (UFP) number concentration, speed, temperature and humidity inside cars. Two sport utility vehicles were used. Three different ventilation conditions were evaluated by up to 20 repeated experiments. In the pre-smoking phase, average in-vehicle PM_2.5 concentrations were 16–17 μg m^?3. Regardless of different window opening conditions, the PM_2.5 levels promptly increased when smoking occurred and decreased after cigarette was extinguished. Although only a single cigarette was smoked, the average PM_2.5 levels were 506–1307 μg m^?3 with different window opening conditions. When smoking was ceased, the average PM_2.5 levels for 15 min were several times higher than the US National Ambient Air Quality Standard of 35 μg m^?3. It took longer than 10 min to reach the level of the pre-smoking phase. Although UFP levels had a similar temporal profile of PM_2.5, the increased levels during the smoking phase were relatively small. This study demonstrated that the SHS exposure in cars with just a single cigarette being smoked could exceed the US EPA NAAQS under realistic window opening conditions. Therefore, the findings support the need for public education against smoking in cars and advocacy for a smoke-free car policy.     

Toxicological response of silver catfish (Rhamdia quelen) after acute exposure to a commercial insecticide containing thiamethoxam

Abstract

This study assessed the hematological, enzymatic and osmoregulatory responses of silver catfish (Rhamdia quelen) exposed to sublethal concentrations (1.125 and 3.750?µg/L) of a commercial thiamethoxam-containing insecticide used on rice crops. Groups of 6 fish per tank (in triplicate, n?=?3, total 54 fish) were exposed for up to 96?h to different concentrations of the compound. After this period, fish were placed in clean water for 48?h. Two fish from each tank (6 per treatment) that had been exposed to the insecticide for 24?h were anesthetized with eugenol and blood was collected to evaluate hematological and biochemical parameters. Blood, liver and muscle were collected for determination of metabolic parameters, plasma cortisol, Cl^-, Na⁺ and K⁺ levels and H⁺-ATPase and Na⁺/K⁺-ATPase activity in the gill. H⁺-ATPase activity was higher in fish exposed to 1.125?µg/L insecticide at 24?h compared to control (0.0?µg/L). Differences in cortisol levels were evidenced throughout the experimental period. These results indicated that exposure to the insecticide changed the hematological, biochemical and metabolic profile of the animals, suggesting concern about environmental safety. Therefore, we discourage the use of this pesticide in areas that come into contact with water bodies inhabited by fish.     

Biodegradation of insecticide carbofuran by Paracoccus sp. YM3

A bacterium (Paracoccus sp. YM3) capable of degrading carbofuran was isolated from carbofuran-contaminated sludge. The strain was shown to metabolize carbofuran (50 mg L^?1) to carbofuran-7-phenol in minimal salt medium within 6 days in which the pesticide was the only source of carbon. Carbofuran and its main metabolite were analyzed by high performance liquid chromatography (HPLC). The addition of an other carbon source led to accelerated biodegradation. The relevant degrading-enzyme was intracellular and inducible. A tobacco hypersensitivity experiment showed that YM3 could eliminate carbofuran in soils effectively and safely. This is the first report of a Paracoccus sp. that could degrade carbofuran. The present study may provide a basis for biotreatment of wastewaters and bioremediation of carbofuran-contaminated soils.     

Characterisation of particulate exposure during fireworks displays

Little is known about the level and content of exposure to fine particles (PM_2.5) among persons who attend fireworks displays and those who live nearby. An evaluation of the levels of PM_2.5 and their elemental content was carried out during the nine launches of the 2007 Montréal International Fireworks Competition. For each event, a prediction of the location of the firework plume was obtained from the Canadian Meteorological Centre (CMC) of the Meteorological Service of Canada. PM_2.5 was measured continuously with a photometer (Sidepak?, TSI) within the predicted plume location (“predicted sites”), and integrated samples were collected using portable personal samplers. An additional sampler was located on a nearby roof (“fixed site”). The elemental composition of the collected PM_2.5 samples from the “predicted sites” was determined using both a non-destructive energy dispersive ED-XRF method and an ICP-MS method with a near-total microwave-assisted acid digestion. The elemental composition of the “fixed site” samples was determined by the ICP-MS with the near-total digestion method. The highest PM_2.5 levels reached nearly 10 000 μg m^?3, roughly 1000 times background levels. Elements such as K, Cl, Al, Mg and Ti were markedly higher in plume-exposed filters. This study shows that 1) persons in the plume and in close proximity to the launch site may be exposed to extremely high levels of PM_2.5 for the duration of the display and, 2) that the plume contains specific elements for which little is known of their acute cardio-respiratory toxicity.     

Biodegradation of insecticide carbofuran by Paracoccus sp. YM3

A bacterium (Paracoccus sp. YM3) capable of degrading carbofuran was isolated from carbofuran-contaminated sludge. The strain was shown to metabolize carbofuran (50 mg L(-1)) to carbofuran-7-phenol in minimal salt medium within 6 days in which the pesticide was the only source of carbon. Carbofuran and its main metabolite were analyzed by high performance liquid chromatography (HPLC). The addition of an other carbon source led to accelerated biodegradation. The relevant degrading-enzyme was intracellular and inducible. A tobacco hypersensitivity experiment showed that YM3 could eliminate carbofuran in soils effectively and safely. This is the first report of a Paracoccus sp. that could degrade carbofuran. The present study may provide a basis for biotreatment of wastewaters and bioremediation of carbofuran-contaminated soils.     

The effect of different ventilation modes on in-vehicle carbon monoxide exposure

In-vehicle carbon monoxide (CO) concentration profiles were monitored in a passenger vehicle driven along a heavily traveled route of a commercial/residential area of Beirut, Lebanon, under several ventilation modes. Trips were conducted during morning rush hours in spring and summer time. Concomitant monitoring of car-exterior CO level, ambient CO level and wind speed was also undertaken. The highest mean CO exposure was experienced for the “windows closed, vents closed” and “windows closed, AC on recirculation” ventilation settings, with mean CO levels of 37.4 and 30.8 ppm, respectively, exceeding the 1-h air quality guidelines. The exposure was less significant for other ventilation modes with respective mean values of $10.8–19\mathrm{ppm}$. Mean car-exterior CO levels were lower than the 1-h air quality guidelines, but exceeded the 8-h CO exposure guidelines. Ambient CO levels were low and non-representative of the personal exposure of individuals neither inside nor in the vicinity of road vehicles. In-vehicle CO levels revealed moderate to good correlations to out-vehicle CO levels for ventilation modes allowing for outdoor air intake, and no correlation to ambient CO levels and wind speed. Infiltration as a result of indoor–outdoor air exchange and intrusion from engine combustion/exhaust infiltration constituted the main sources of observed in-vehicle CO levels.     

Toxicological effect of MPA-CdSe QDs exposure on zebrafish embryo and larvae

Cadmium selenium (CdSe) quantum dots (QDs) are semiconductor nanocrystals that hold wide range of applications and substantial production volumes. Due to unique composition and nanoscale properties, their potential toxicity to aquatic organisms has increasingly gained a great amount of interest. However, the impact of CdSe QDs exposure on zebrafish embryo and larvae remains almost unknown. Therefore, the lab study was performed to determine the developmental and behavioral toxicities to zebrafish under continuous exposure to low level CdSe QDs (0.05-31.25 mg L⁻¹) coated with mercaptopropionic acid (MPA). The results showed MPA-CdSe exposure from embryo to larvae stage affected overall fitness. Our findings for the first time revealed that: (1) The 120 h LC₅₀ of MPA-CdSe for zebrafish was 1.98 mg L⁻¹; (2) embryos exposed to MPA-CdSe resulted in malformations incidence and lower hatch rate; (3) abnormal vascular of FLI-1 transgenic zebrafish larvae appeared after exposure to MPA-CdSe including vascular junction, bifurcation, crossing and particle appearance; (4) larvae behavior assessment showed during MPA-CdSe exposure a rapid transition from light-to-dark elicited a similar, brief burst and a higher basal swimming rate; (5) MPA-CdSe induced embryos cell apoptosis in the head and tail region. Results of the observations provide a basic understanding of MPA-CdSe toxicity to aquatic organisms and suggest the need for additional research to identify the toxicological mechanism.     

S-metolachlor pulse exposure on the alga Scenedesmus vacuolatus: effects during exposure and the subsequent recovery

In streams and creeks, the aquatic flora is exposed to fluctuating concentrations of herbicides during and following their application. Peak concentrations of herbicides, like the chloroacetanilide S-metolachlor, are usually detected following rain events. In this study, we assessed the effect of S-metolachlor pulse exposure on the algae Scenedesmus vacuolatus. We measured the time-dependency of effects during exposure on algae population and identified the algae development stage most sensitive to S-metolachlor. Furthermore, we assessed the time-to-recovery of the algae following exposure. A 6h pulse exposure at 598mugl(-1) was sufficient to inhibit cell reproduction by 50%. However, the exposure period had to coincide with the cell development stage specifically inhibited by S-metolachlor, which is the end of the cell growth phase. In algae populations composed of cells at all development stages, we initially observed an increase in the size of some algal cells, ultimately leading to an inhibition of the growth rate. In these experimental conditions, effects were observed after 18h of exposure and greatly increased with time. The recovery of algae following exposure to strongly inhibiting S-metolachlor concentrations was delayed and only occurred after 29h. These findings suggest that peak exposure to S-metolachlor may affect the growth of sensitive alga in surface waters, considering that the effects extend beyond the period of exposure.     

A chamberless field exposure system for ozone enrichment of short vegetation

Only few studies have been conducted as yet which focus on the effects of rising tropospheric ozone levels on semi-natural vegetation under free-air conditions. A new technical approach was used to examine the response of calcareous grassland to ozone employing a chamberless fumigation system. Four different ozone regimes were applied (1-, 1.33-, 1.66- and 2-fold ambient air levels) with five replicates each. Ozone enrichment was carried out on circular plots of 2 m in diameter by a computer controlled exposure system. Transparent windscreens encircling each plot accelerated the mixing of ambient air and ozone released. Thus, the use of blowers could be avoided. The exposure system presented here is regarded as an appropriate technique for free-air trace gas enrichment on short vegetation avoiding microclimatic alterations known to affect plant growth and pollutant uptake. Furthermore, the chosen technical set-up was rather cost-effective. Hence, it enabled the establishment of a larger number of replications providing the basis for results of higher statistical power.     

The effect of high UV radiation exposure environment on the novel PVC polymers

Environmental Science and Pollution Research - Although plastic induces environmental damages, almost the consumption of poly(vinyl chloride) never stops increasing. Therefore, this work abstracted...