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1.
Previously we have shown that 1-arylpropane-1,2-diols are catabolic products of L-phenylalanine during idiophasic metabolism of B. adusta that are stereoselectively biosynthesized from a C(7)-unit (ring+benzylic carbon) and a C(2)-unit as predominantly erythro 1R, 2S enantiomers.In order to probe the mechanism of 1-arylpropane-1,2-diol formation, the products of the incubation of isotopically labelled aromatic aldehydes as substrates with Bjerkandera adusta (DAOM 215869) have been characterized. The aromatic aldehydes were benzaldehyde (ring D(5)) and 4-methoxy- and 4-hydroxybenzaldehydes (ring 13C(6)). These aldehydes were all stereoselectively incorporated into the corresponding 1-arylpropane-1,2-diols, including the chloro analogues, as well as into the corresponding alpha-ketols (phenyl acetyl carbinols (PAC's) and 2-hydroxy propiophenones (2-HPP's)) the presumed precursors of the diols. Benzoic acid (ring D(5)) was likewise incorporated into the diols, chlorodiols and alpha-ketols.These results lead us to conclude that the aromatic aldehydes benzaldehyde, 4-hydroxybenzaldehyde and 4-methoxybenzaldehyde are likely C(7)-unit precursors in the carboligation reaction(s) that leads to 1-arylpropane-1,2-diol biosynthesis. The metabolic role of the diols remains to be elucidated but they may be important intermediates in CAM (chlorinated anisyl metabolite) aldehyde-alcohol cycling and also act as substrates for the chlorination/hydroxylation enzymes yet to be identified in white rot fungi. 相似文献
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综述了白腐真菌在不同反应器中合成木质素降解酶系能力的研究进展,包括培养方式与条件、不同反应器的特点及应用、木质素降解酶系的应用及前景. 相似文献
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综述了白腐真菌在不同反应器中合成木质素降解酶系能力的研究进展,包括培养方式与条件、不同反应器的特点及应用、木质素降解酶系的应用及前景。 相似文献
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PCB congener selective biodegradation by the white rot fungus Pleurotus ostreatus in contaminated soil 总被引:6,自引:0,他引:6
Six strains of white rot fungi were tested for their biodegradation ability of low chlorinated polychlorinated biphenyl (PCB) commercial mixture (Delor 103) in real soil system. Phanerochaete chrysosporium and Trametes versicolor did not show any ability to degrade PCBs in soil. On the contrary, four strains of Pleurotus ostreatus were able to remove about 40% of Delor 103 in two months. All P. ostreatus strains decomposed PCBs selectively with the preference for congeners with chlorine atoms in ortho > meta > para position. Degradation efficiency decreased with increasing number of chlorination. 相似文献
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为利用微生物技术去除环境中的氯丹等有机氯农药污染提供依据,研究了4种Tween系列非离子表面活性剂对白腐菌Phlebia lindtneri GB1027降解有机氯农药氯丹及其中间代谢产物的强化效果。结果表明,表面活性剂对氯丹的增溶效果明显,在浓度为2.0 g/L的Tween20、Tween40、Tween60和Tween80溶液中,氯丹的溶解度与在水中的溶解度相比分别提高了16.25、18.21、20.36和20.89倍。低浓度(0.05 ~ 1.0 g/L)的表面活性剂对白腐菌在PDB培养基中的生长具有促进作用,而2.0 g/L的表面活性剂则会抑制菌株的生长。在0.05 ~ 1.0 g/L浓度范围内,各表面活性剂可促进白腐菌对氯丹及其代谢产物的降解效果,且降解率随着表面活性剂浓度的升高而增加。其中,白腐菌在添加了1.0 g/L的Tween80的体系中培养15 d后,对氯丹、氧化氯丹和环氧七氯的降解率分别比对照提高了30.2%、48.0%和34.3%。而当表面活性剂浓度升高至2.0 g/L时,由于菌株的生长受到抑制而导致对降解的促进作用不明显,甚至下降。 相似文献
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白腐菌P.sordida YK-624及其锰过氧化物酶对2-氯酚的转化 总被引:1,自引:0,他引:1
利用白腐菌P.sordida YK-624和产自该菌株的锰过氧化物酶(MnP)处理2-氯酚(2-CP).结果表明,当2-CP质量浓度为50mg/L时,培养5d, P.sordida YK-624能降解50.42%的2-CP;采用300 U的MnP处理150mg/L的2-CP, 24h时2-CP降解率可达75.00%.利用紫外-可见分光光度计、红外光谱仪和高效液相色谱仪对MnP处理过程中的产物进行了分析,表明MnP氧化2-CP过程中有多种产物生成,可能的产物包括醌类和聚合产物. 相似文献
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Irgarol 1051 (2-methylthio-4-tert-butylamino-6-cyclopropylamino-s-triazine), a derivative of s-triazine herbicide, is an antifouling compound used as an alternative to organotins. The compound is highly persistent and is known to be biodegraded only by the white rot fungus, Phanerochaete chrysosporium. We used partially purified manganese peroxidase (MnP) prepared from P. chrysosporium to evaluate its capacity to degrade Irgarol 1051. MnP degraded Irgarol 1051 to two major products, one identified as M1 (identical to GS26575, 2-methylthio-4-tert-butylamino-6-amino-s-triazine) and the other not identified but with same mass spectrum as M1 and a different ultraviolet spectrum. This report clearly demonstrates that this ligninolytic enzyme is involved in the degradation of Irgarol 1051. 相似文献
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Evaluation of the individuality of white rot macro fungus for the decolorization of synthetic dye 总被引:1,自引:0,他引:1
Priyanka Pandey Ram Praksh Singh Kailash Nath Singh Paramasivam Manisankar 《Environmental science and pollution research international》2013,20(1):238-249
Introduction
A biosorbent was developed by simple dried Agaricus bisporus (SDAB) and effectively used for the biosorption of cationic dyes, Crystal Violet and Brilliant Green.Materials and methods
For the evaluation of the biosorbent system, all the batch equilibrium parameters like pH, biomass dose, contact time, and temperature were optimized to determine the decolorization efficiency of the biosorbent. The maximum yields of dye removal were achieved at pH 4.0 for Crystal Violet (CV) and pH 5.0 for Brilliant Green (BG), which are closer to their natural pH also.Result and discussion
Equilibrium was established at 60 and 40 min for CV and BG, respectively. Pseudo first-order, pseudo second-order, and intraparticle-diffusion kinetic models were studied at different temperatures. Isotherm models such as Freundlich, Langmuir, and Dubinin–Radushkevich were also studied. Biosorption processes were successfully described by Langmuir isotherm model and the pseudo second-order kinetic model.Conclusions
The biosorption capacity of A. bisporus over CV and BG were found as 21.74 and 12.16 mg gm?1. Thermodynamic parameters indicated that the CV and BG dye adsorption onto A. bisporus is spontaneous and exothermic in the single and ternary systems. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy were used for the surface morphology, crystalline structure of biosorbent, and dye–biosorbent interaction, respectively. This analysis of the biosorption data confirmed that these biosorption processes are ecofriendly and economical. Thus, this biomass system may be useful for the removal of contaminating cationic dyes. 相似文献14.
开放体系下稻草-白腐菌对染料的吸附脱色与降解 总被引:1,自引:0,他引:1
在开放体系下,研究了稻草-白腐菌对孔雀绿和结晶紫的吸附脱色与降解.结果表明,稻草能有效地对孔雀绿和结晶紫进行吸附脱色,其中稻草用量、粒径和pH对稻草吸附染料有一定的影响,在合适条件下稻草对孔雀绿和结晶紫的最大吸附量分别可达到31.89、52.98mg/g;在吸附孔雀绿和结晶紫的稻草中接入白腐菌,发现开放体系下白腐菌能对两种染料进行降解,6d后,孔雀绿和结晶紫的脱色率分别达到93.67%和80.OO%;再生后的稻草基质对孔雀绿和结晶紫也有较好的吸附,最大再生率分别达到74.45%和43.34%.降解过程中白腐菌分泌酶的酶活力与染料及稻草基质降解没有直接的联系. 相似文献
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Lee SM Lee JW Park KR Hong EJ Jeung EB Kim MK Kang HY Choi IG 《Journal of environmental science and health. Part. B》2006,41(4):385-397
The white rot fungus Stereum hirsutum was used to degrade methoxychlor [2,2,2-trichloro-1,1-bis(4-methoxyphenyl)ethane] in culture and the degraded products were extensively determined. The estrogenic activity of the degraded products of methoxychlor was examined using cell proliferation and pS2 gene expression assays in MCF-7 cells. S. hirsutum showed high resistance to methoxychlor 100 ppm, and the mycelial growth was fully completed within 8 days of incubation at 30 degrees C. Methoxychlor in liquid culture medium was gradually converted into 2,2-dichloro-1,1-bis(4-methoxyphenyl)ethane, 2,2-dichloro-1,1-bis(4-methoxyphenyl)ethylene, 2-chloro-1,1-bis(4-methoxyphenyl) ethane, 2-chloro-1,1-bis(4-methoxyphenyl) ethylene, and 1,1-bis(4-methoxyphenyl)ethylene, indicating that methoxychlor is dominantly degraded by dechlorination and dehydrogenation. MCF-7 cells were demonstrated to proliferate actively at the 10-5 M concentration of methoxychlor. However, cell proliferation was significantly inhibited by the incubation with methoxychlor culture media containing S. hirsutum. In addition, the expression level of pS2 mRNA was increased at the concentration (10-5 M) of methoxychlor. The reductive effect of S. hirsutum for methoxychlor was clear but not significant as in the proliferation assay. 相似文献
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Dichomitus squalens efficiently decolorized both Orange G and Remazol Brilliant Blue R (RBBR) at concentrations of 0.5gl(-1) and 3gl(-1) in static and shaken culture and also on solid medium within 14d. The presence of the dyes in the culture medium mostly caused a decrease in biomass production and in growth rate, which was more significant in the case of RBBR. After 14d of cultivation, electron microscopy showed substantial morphological changes in mycelia of D. squalens growing in media containing dyes. The hyphae deformations were more intensively manifested in solid media than in liquid culture. In all the cases, the morphological changes were more prominent in the presence of RBBR. Higher concentrations of both dyes brought about more intensive changes. The toxicity of synthetic dyes Orange G and RBBR was tested using a bioassay based on the growth inhibition of duckweed Lemna minor. Two endpoints such as the number of fronds and their weight were studied during the bioassay. The results showed higher toxicity of RBBR than that of Orange G. The toxicity of both dyes decreased after the decolorization process. 相似文献
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重金属和有机物复合污染是水处理中的难题。本研究发现一株白腐真菌Ganoderma Sinensis不仅可以通过富集作用去除重金属镉(Cd),而且对蒽具有降解能力。当Cd的浓度为10~30 mg/L时,该菌株对Cd的去除率为6.78%~21.9%;当蒽的浓度为10~30 mg/L时,菌株对蒽的降解率为18.25%~33.3%;当Cd和蒽同时存在于培养基中时,随着Cd浓度的增加(10~30 mg/L),对菌株的生物量及蒽降解的抑制作用也在增大;而随着蒽浓度的增加(10~30 mg/L),菌株的生物量及Cd的富集能力并未受到显著的影响。实验结果表明,该菌株有望用于镉和蒽复合污染的水体处理。 相似文献
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Bioremediation of crude oil polluted soil by the white rot fungus, <Emphasis Type="Italic">Pleurotus tuberregium</Emphasis> (Fr.) Sing. 总被引:1,自引:0,他引:1
Isikhuemhen OS Anoliefo GO Oghale OI 《Environmental science and pollution research international》2003,10(2):108-112
Bioremediation has become an attractive alternative to physicochemical methods of remediation of polluted sites. White rot fungi (WRF) are increasingly being investigated and used in bioremediation, because of their ability to degrade an extremely diverse range of very persistent or toxic environmental pollutants. The white rot fungus, Pleurotus tuberregium, was examined for its ability to ameliorate crude oil polluted soil. This was inferred from the ability of the polluted soil to support seed germination and seedling growth in Vigna unguiculata, at 0, 7 and 14 days post treatment. Results obtained from the present study showed that bioremediation of soil contaminated with crude oil was possible, especially when the fungus had been allowed to establish and fully colonize the substrate mixed with the soil. There were significant improvements in % germination, plant height and root elongation values of test plants, when seeds were planted 14 days post soil treatment. At 1 to 5% crude oil pollution, % germination values were comparable with the values in control plants in the 14 days treatment, and significantly higher than values obtained in the day 0 treatment. Also, at the highest level of crude oil pollution (15%), there was about 25% improvement in % germination value over the 0 day treatment. This trend of improvement in values was also observed for plant height, root elongation and biomass accumulation as well as decreased total hydrocarbon content. 相似文献
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共基质对白腐菌降解喹啉的影响 总被引:1,自引:1,他引:0
选用吲哚、苯酚和氨氮作为喹啉降解的共基质.通过白腐菌BP对共基质的降解,研究了白腐菌BP对不同共基质降解体系中喹啉的降解过程及其反应动力学,同时研究了共基质物质对白腐菌BP漆酶活力、生物量增长速率和降解体系pH的影响.结果显示,白腐菌BP对不同共基质降解体系中的喹啉均具有较高的降解率,共基质物质苯酚、吲哚和氨氮对喹啉的降解有一定的抑制作用;共基质降解体系使白腐菌BP漆酶系统启动更加迅速,漆酶活力峰值提前出现;共基质降解体系能促进白腐菌BP的生长,缩短白腐菌BP的生长周期;秸秆滤出液培养基中,pH为6.00~8.00时,白腐菌BP对喹啉均具有较强的降解能力. 相似文献