Changes in total cyanide content of tissues from cassava plants infested by mites (Mononychellus tanajoa) and mealybugs (Phenacoccus manihoti)

Dietary and industrial uses of cassava (Manihot esculenta Crantz) are threatened by the known presence of cyanide in the crop and its products. The toxic HCN content of cassava may be altered by some intrinsic and extrinsic factors in and around the plant, but the extent of any shifts in this regard due to stress conditions created by cassava green spider mites (CGM) Mononychellus tanajoa and mealybugs (CM) (Phenacoccus manihoti, now spreading epiphytotically in almost all cassava-growing areas in Africa, is unknown. In this study, we report variations in the total HCN content of leaf, stem and root tissues of six cassava clones differentially infested by these pests.     

Observations on the economic importance of tomato leaf miner (Liriomyza bryoniae) (agromyzidae)

Yield losses can occur as a result of leaf miner infestation, but are not dependent on severity of damage alone; proximity to fruit at an early stage of development is very important. If damage on leaves adjacent to a truss reached 30 mines/leaf at the time fruit was half-swollen, a 10% loss of yield resulted. At three levels of infestation investigated, losses amounted to 5, 13 and 20% of monetary returns. Although it was found that growing soft plants and keeping de-leafing to the minimum would maximise yields, it appeared that the direct effect of these cultural practices on yield was more important than their indirect effects on leaf miner damage.     

The role of soil organic matter in maintaining the productivity and yield stability of cereals in China

The role of soil organic matter (SOM) in agricultural systems has been widely studied in conjunction with the potential for greenhouse gas mitigation. However, the link between SOM accumulation in croplands, crop productivity and yield stability has not yet been clearly established. In this paper, we collected data on provincial yearly crop productivity (yields, total cropland area) during 1949–1998 and the average SOM contents in croplands sampled and determined from the National Soil Survey in 1979–1982 of mainland China. The cereal productivity was assessed both with an overall mean of 1949–1998 and with the mean values for different time periods within this overall time, respectively. The yield variability within a single stabilizing stage, and between the fluctuating years, was calculated as a negative measure of yield stability. The correlation between SOM and cereal productivity was very significant for most provinces, but the relationship has become less significant as we approach the present. Moreover, the average yield variability was very significantly and negatively correlated with the cropland SOM level. The findings support our previous hypothesis from case studies, that C sequestration in China's croplands may provide win–win benefits, by enhancing crop productivity and stabilizing yield. This offers a sound basis as a greenhouse gas mitigation strategy by promoting C sequestration in croplands, and enhancing food security in China's agriculture.     

Effects of height and habitat type on egg parasitism by Trichogramma minutum and T. pretiosum (Hymenoptera: Trichogrammatidae)

Heliothis virescens (Fabricius) eggs were sampled to determine the effects of height and habitat type (soybean field vs. uncultivated vegetation) on rates of p arasitism by Trichogramma minutum Riley and T. pretiosum Riley. The potentially confounding effects of plant associations and host density and distribution were eliminated by sampling with laboratory-produced eggs deposited on cloths, rather than with eggs naturally deposited by endemic hosts.A 3-way contingency table analysis showed that parasitism (whether the egg cloths were parasitized or not) was significantly associated with height and habitat type in 1981, with higher rates of parasitism in the uncultivated habitat and at the greater height. Parasitism was independent of height and habitat in 1982. Rates of parasitism by the two Trichogramma species were not clearly associated with habitat type, but were associated with height. T. minutum consistently parasitized egg cloths positioned at greater heights than those parasitized by T. pretiosum. While the behavioral mechanisms responsible are unknown, this inter-specific difference could have important implications with regard to selecting the most suitable of these two Trichogramma species for a particular inundative release program.     

Toxic effects of perfluorononanoic acid on the development of Zebrafish (Danio rerio) embryos

Perfluorononanoic acid (PFNA) is a nine-carbon perfluoroalkyl acid widely used in industrial and domestic products. It is a persistent organic pollutant found in the environment as well as in the tissues of humans and wildlife. There is a concern that this chemical might be a developmental toxicant and teratogen in various ecosystems. In the present study, the toxic effects of PFNA were evaluated in zebrafish (Danio rerio) embryos. One hour post-fertilization embryos were treated with 0, 25, 50, 100, 200, 300, 350, and 400 μmol/L PFNA for 96 hr in 6-well plates. Developmental phenotypes and hatching rates were observed and recorded. Nineteen genes related to oxidative stress and lipid metabolism were examined using Quantitative RT-PCR and confirmed by whole mount in situ hybridization (WISH). Results showed that PFNA delayed the development of zebrafish embryos, reduced the hatching rate, and caused ventricular edema and malformation of the spine. In addition, the amount of reactive oxygen species in the embryo bodies increased significantly after exposure to PFNA compared with that of the control group. The Quantitative RT-PCR and WISH experiments demonstrated that mRNA expression of the lfabp and ucp2 genes increased significantly while that of sod1 and mt-nd1 decreased significantly after PFNA exposure. The mRNA expression levels of gpx1 and mt-atp6 decreased significantly in the high concentration group. However, the mRNA expression levels of both ppara and pparg did not show any significant variation after exposure. These findings suggest that PFNA affected the development of zebrafish embryos at relatively low concentrations.     

Effect of herbivory by the slug, Mariaella dussumieri, and certain insects on growth and competitive success of two sympatric annual weeds

The effect of plant-eating by the slug, Mariaella dussimieri Gray, and by certain lepidopteran insects and their larvae was studied under field conditions, on the growth of two sympatric annual weeds, Galinsoga ciliata (Raf.) Blake and G. parviflora Cav., in pure and mixed stands. Under the influence of herbivory, both weeds generally produced a greater number of leaves, but the leaf area per plant was drastically reduced. Plant survival and seed output decreased in both weeds due to herbivory, but the effect was more severe in G. parviflora, especially in the mixed stands. Herbivory also reduced the dry matter yield and delayed flowering in G. parviflora. Relatively poor growth of G. parviflora in the mixed stand exposed to herbivory could be attributed to its preferential feeding by the slug and insects which reduced the competitive ability of this weed against G. ciliata.     

Seasonal occurrence of the yellow stem borer Scirpophaga incertulas (Walker) on deepwater rice in Bangladesh and Thailand

Deepwater rice is grown in South and Southeast Asia on 11 million hectares flooded deeply during the monsoon. Surveys for the incidence of stem borers in deepwater rice in Bangladesh from 1977 to 1980, and in Thailand during 1981 and 1982, showed that borer incidence and species composition were remarkably similar. Scirpophaga incertulas (Walker) usually comprised more than 90% of the borer population and was almost exclusively present during the main flooding period. Chilo polychrysus (Meyr.) comprised 11% and Sesamia inferens (Walker) 6% of the population in the pre-flood and ripening stages.Low borer activity in the early part of the season was explained by a low nucleus population, the synchronous planting of deepwater rice, and severe moisture stress and high temperatures in the pre-flood period. In Bangladesh, a severe drought in 1979 limited Scirpophaga incertulas populations to unusually low levels until the flowering stage. In Thailand, drier conditions, the lack of an intervening rice crop in the dry season and a longer pre-flood period, appeared to severely restrict early season borer activity. The onset of flooding and stem elongation provided a more favourable environment for S. incertulas. Borer activity increased steadily during the first 3–4 months of flooding, to average 23% damaged stems (26 m^?2) and 13 borer immature stages/100 stems (13 m^?2) by the flowering stage. Borer activity continued at about the same level as the water receded, to reach maximum annual levels of 38–44% damaged stems (35 m^?2) at the late-ripening stage. At harvest, 60% of the fields were at outbreak level (>40% damaged stems). Such high levels of damage are explained by the long growth period of deepwater rice, favourable weather during the flooding period, and the optimum habitat of the elongated stem. S. incertulas populations then crashed to low levels; the larvae entering diapause in the field stubble. Although related to actual damage, the deadheart and whitehead symptoms grossly under-estimated the extent of stem damage.     

Oviposition markers in Callosobruchus maculatus F. and Callosobruchus rhodesianus pic. (coleoptera,bruchidae): Assymmetry of interspecific responses

The bean weevils Callosobruchus maculatus (F.) and C. rhodesianus (Pic.) both laid fewer eggs on previously infested adzuki beans than on fresh beans, even after eggs were removed. A variety of experimental treatments demonstrated the existence of an oviposition marker with ether soluble components. Callosobruchus rhodesianus was inhibited from ovipositing by the C. maculatus marker, but not vice versa.     

Tapping practices and SAP yields of the NIPA palm (NIPA Fruticans) in Papua New Guinea

Methods of tapping the nipa palm (Nipa fruticans Wurmb.) were developed in the course of a 3-year study conducted in seven villages in the Baimuru district of the Gulf Province of Papua New Guinea. The experimental sites were located about 1.5–23 km from the sea. Their plant density varied from 4700 to 2400 palms per ha and the mean height of the plants was nearly 13 m.Five pre-treatment patterns were tested. Once-a-week treatment, maintained for 12 weeks, yielded an average of 155 ml of sap/24 h/palm for one month, whereas 3–5-times-a-week treatment, for 5–12 weeks, yielded an average of 1300 ml of sap. The duration of treatment depended on the state of development of the flower stalk. The pre-treatment pattern chosen as the standard was to bend the stalk 12 times in one direction, to pat with the hands the length of the stalk backwards and forwards 64 times, and to give the base four kicks; this was repeated four times a week.In the course of the 3-year study it was found that the average sugar content of fresh nipa sap is rather high, about 16.4% w/v, as compared with, for example, about 12% w/v of sugar cane. The results of the present study are discussed with reference to producing fuel alcohol from the Papua New Guinea nipa palm.     

The role of environmental management system on introduction of new technologies in the metal and chemical/paper/plastics industries

Pollution prevention with the use of modern cleaner technologies in industrial sectors is the cornerstone of successful environmental policy certified according to the requirements of the international standard ISO 14001. The analyses were performed with the objective of assessing general aspects of technology modernisation as a result of the ISO 14001 certification in industrial enterprises in order to develop a better understanding whether the ISO 14001 certification can accelerate initiatives for the adoption of new and cleaner technologies within the certified firms on one hand, and, on the other hand, to find out to what extent it helped to upgrade their environmental performance. The research was performed within Slovene metal and chemical (including pharmaceuticals, paper and plastics) manufacturing companies with an additional emphasis on firms which are committed to implementing the IPPC directive. In general, certified enterprises consider ISO 14001 as a very useful tool in promoting and adopting new cleaner technologies. ISO 14001 seems to be particularly important to create better conditions for the technology changes in companies which are committed to the IPPC Directive. Companies in chemical and related industries, to a much higher extent, used predominantly modified technologies to diminish their environmental impacts, while companies in metal industries, to a higher extent, used a combination of existing and new technologies after ISO 14001 certification. It seems that better environmental performance is associated with higher productivity in ISO 14001 certified firms.     

The role of two distinct subunit types in the architecture of keyhole limpet hemocyanin (KLH)

The Science of Nature -     

Response of the bird cherry-oat aphid (Rhopalosiphum padi) to climate change in relation to its pest status, vectoring potential and function in a crop-vector-virus pathosystem

Global climate change threatens world food production via direct effects on plant growth and alterations to pest and pathogen prevalence and distribution. Complex relationships between host plant, pest, pathogen and environment create uncertainty particularly involving vector-borne diseases. We attempt to improve the understanding of the effects of climate change via a detailed review of one crop-vector-pathogen system.The bird cherry-oat aphid, Rhopalosiphum padi, is a global pest of cereals and vector of yellow dwarf viruses that cause significant crop losses in cereals. R. padi exhibits both sexual and parthenogenetic reproduction, alternating between crops and other host plants. In Australia, only parthenogenesis occurs due to the absence of the primary host, thus the aphid continuously cycles from grasses to cereals, allowing for continuous virus acquisition and transmission.We have reviewed the potential impact of future climate projections on R. padi population dynamics, persistence, abundance, dispersal and migration events as well as the interactions between vector, virus, crop and environment, all of which are critical to the behaviour and development of the vector and its ability to transmit the virus. We identify a number of knowledge gaps that currently limit efforts to determine how this pathosystem will function in a future climate.     

Breeding skylarks (Alauda arvensis) on organic and conventional arable farms in The Netherlands

The aim of this study was to analyse the effects of differences in cropping pattern between organic and conventional arable farms on the breeding activity of skylarks and to assess the effects of arable crop management on skylark nest survival. Skylark nest density was seven times higher on organic farms than on conventional farms (0.63 vs. 0.09 nest per 10 ha). Skylarks showed a strong preference for spring cereals, lucerne and grass leys, all of which were mainly or exclusively grown on organic farms. On organic farms nests were initiated during the entire breeding season, but on conventional farms no nesting activity was found during the peak of the season (early May to early June). On organic farms 27% of all nests was successful. Increasing the availability of suitable breeding habitat during the peak of the breeding season on conventional farms might provide one means of enhancing breeding skylark populations. On organic farms, crop management should focus on reducing nest loss due to farming operations.     

Effects of two polybrominated diphenyl ethers (BDE-47, BDE-209) on the swimming behavior,population growth and reproduction of the rotifer Brachionus plicatilis

Polybrominated diphenyl ethers (PBDEs) are new kinds of persistent organic pollutants (POPs) and their potential threats to the equilibrium and sustainability of marine ecosystems have raised worldwide concerns. Here, two kinds of PBDEs, tetra-BDE (BDE-47) and deca-BDE (BDE-209) were applied, and their toxic effects on the swimming behavior, population growth and reproduction of Brachionus plicatilis were investigated. The results showed that: (1) The actual concentrations of BDE-47 and -209 in the seawater phase measured by GC–MS (Gas Chromatography−Mass Spectrometer) were much lower than their nominal concentrations. (2) In accordance with the 24-hr acute tests, BDE-209 did not show any obvious swimming inhibition to rotifers, but a good correlation did exist between the swimming inhibition rate and BDE-47 concentration suggesting that BDE-47 is more toxic than BDE-209. (3) Both BDE-47 and -209 had a significant influence on the population growth and reproduction parameters of B. plicatilis including the population growth rate, the ratio of ovigerous females/non-ovigerous females (OF/NOF), the ratio of mictic females/amictic females (MF/AF), resting egg production and the mictic rate, which indicate that these parameters in B. plicatilis population were suitable for monitoring and assessing PBDEs. Our results suggest that BDE-47 and -209 are not acute lethal toxicants and may pose a low risk to marine rotifers at environmental concentrations for short-term exposure. They also accumulate differently into rotifers. Further research data are needed to understand the mechanisms responsible for the effects caused by PBDEs and to assess their risks accurately.     

The mammal assemblage of the hominid site TM266 (Late Miocene, Chad Basin): ecological structure and paleoenvironmental implications

Characterizing the paleoenvironmental context of the first hominids is a key issue for understanding their behavioral and morphological evolution. The present study aims at reconstructing the paleoenvironment of the TM266 vertebrate assemblage (Toros-Menalla, Northern Chad) that yielded the earliest known hominid Sahelanthropus tchadensis (7 Ma). For the first time, a quantitative analysis is carried out on the fossil mammal assemblage associated with that hominid. Two complementary approaches were applied: (1) the analysis of the relative abundances of taxa and their habitat preferences; and (2) the study of the distribution of taxa within three meaningful ecovariables: locomotion, feeding preferences, and body mass. The resulting taxonomic and paleoecological structures are used to reconstruct the diversity and the relative extent of the habitats in that part of northern Chad seven million years ago. The paleoenvironment was composed of open areas with dry and humid grasslands, prevailing over wooded habitats. Water was also widely available as freshwater bodies and certainly swamps. It appears that the high habitat diversity of the landscape is a common feature among paleoenvironments associated with early hominids.     

Explorations on the University's role in society for sustainable development through a systems transition approach. Case-study of the Technical University of Catalonia (UPC)

Focusing on the sustainability challenge, a growing number of Universities are redefining their core strategies in order to adapt to the real social needs and expectations of their stakeholders. Beyond integrating sustainability into the University's internal activities (curriculum, research, operations, etc.), the challenge seems to be integrating the University with the sustainable development paths of society. For the last 10 years the Technical University of Catalonia (UPC) has tackled this challenge through an overarching strategic plan. The latest strategy, UPC Sustainable Plan 2015 has adopted a different focus, which was designed through a participatory approach, involving many internal and external stakeholders.This work aims to use a conceptual framework based on systems transitions to assess the systems change potential of a University strategy, in this case, the sustainability strategy of UPC. The main objectives of this paper are: to present the sustainability strategy participatory design process and its outcomes at UPC; to analyse the strategy change potential through a new transition/systems renewal method that is proposed; and to compare it with the UPCs previous environmental strategy; The work concludes that the systems transition potential is currently higher with the new strategy and discusses the elements that explain this finding.     

Gravity orientation in social wasp comb cells (Vespinae) and the possible role of embedded minerals

Social wasps and hornets maintain their nest in the dark. The building of the combs by all Vespinae is always in the direction of the gravitational force of Earth, and in each cell's ceiling, at least one 'keystone' is embedded and fastened by saliva. The sensory mechanisms that enable both building of sizeable symmetrical combs and nursing of the brood in the darkness merit investigation, and the aim of the present study was to identify and characterize the 'keystones' that exist in the ceiling and in the walls of the social wasp comb cells. Bio-ferrography was used to isolate magnetic particles on slides. These slides, as well as original cells, were analyzed in an environmental scanning electron microscope by a variety of analytical tools. It was found that both the roof and the walls of each comb cell bear minerals, like ferrites, as well as Ti and Zr. The latter two elements are less abundant in the soil around the nest. Ti and Zr are known to reflect infrared (IR) light. IR imaging showed a thermoregulatory center in the dorsal thorax of the adult Oriental hornet. It is not known yet whether these insects can sense IR light.