A novel statistical method for classifying habitat generalists and specialists

We develop a novel statistical approach for classifying generalists and specialists in two distinct habitats. Using a multinomial model based on estimated species relative abundance in two habitats, our method minimizes bias due to differences in sampling intensities between two habitat types as well as bias due to insufficient sampling within each habitat. The method permits a robust statistical classification of habitat specialists and generalists, without excluding rare species a priori. Based on a user-defined specialization threshold, the model classifies species into one of four groups: (1) generalist; (2) habitat A specialist; (3) habitat B specialist; and (4) too rare to classify with confidence. We illustrate our multinomial classification method using two contrasting data sets: (1) bird abundance in woodland and heath habitats in southeastern Australia and (2) tree abundance in second-growth (SG) and old-growth (OG) rain forests in the Caribbean lowlands of northeastern Costa Rica. We evaluate the multinomial model in detail for the tree data set. Our results for birds were highly concordant with a previous nonstatistical classification, but our method classified a higher fraction (57.7%) of bird species with statistical confidence. Based on a conservative specialization threshold and adjustment for multiple comparisons, 64.4% of tree species in the full sample were too rare to classify with confidence. Among the species classified, OG specialists constituted the largest class (40.6%), followed by generalist tree species (36.7%) and SG specialists (22.7%). The multinomial model was more sensitive than indicator value analysis or abundance-based phi coefficient indices in detecting habitat specialists and also detects generalists statistically. Classification of specialists and generalists based on rarefied subsamples was highly consistent with classification based on the full sample, even for sampling percentages as low as 20%. Major advantages of the new method are (1) its ability to distinguish habitat generalists (species with no significant habitat affinity) from species that are simply too rare to classify and (2) applicability to a single representative sample or a single pooled set of representative samples from each of two habitat types. The method as currently developed can be applied to no more than two habitats at a time.     

Effects of Landscape Composition and Wetland Fragmentation on Frog and Toad Abundance and Species Richness in Iowa and Wisconsin, U.S.A.

Abstract: Management of amphibian populations to reverse recent declines will require defining high-quality habitat for individual species or groups of species, followed by efforts to retain or restore these habitats on the landscape. We examined landscape-level habitat relationships for frogs and toads by measuring associations between relative abundance and species richness based on survey data derived from anuran calls and features of land-cover maps for Iowa and Wisconsin. The most consistent result across all anuran guilds was a negative association with the presence of urban land. Upland and wetland forests and emergent wetlands tended to be positively associated with anurans. Landscape metrics that represent edges and patch diversity also had generally positive associations, indicating that anurans benefit from a complex of habitats that include wetlands. In Iowa the most significant associations with relative abundance were the length of the edge between wetland and forest ( positive) and the presence of urban land (negative). In Wisconsin the two most significant associations with relative abundance were forest area and agricultural area ( both positive). Anurans had positive associations with agriculture in Wisconsin but not in Iowa. Remnant forest patches in agricultural landscapes may be providing refuges for some anuran species. Differences in anuran associations with deep water and permanent wetlands between the two states suggest opportunities for management action. Large-scale maps can contribute to predictive models of amphibian habitat use, but water quality and vegetation information collected from individual wetlands will likely be needed to strengthen those predictions. Landscape habitat analyses provide a framework for future experimental and intensive research on specific factors affecting the health of anurans.     

Bird‐community responses to habitat creation in a long‐term,large‐scale natural experiment

Ecosystem function and resilience are compromised when habitats become fragmented due to land‐use change. This has led to national and international conservation strategies aimed at restoring habitat extent and improving functional connectivity (i.e., maintaining dispersal processes). However, biodiversity responses to landscape‐scale habitat creation and the relative importance of spatial and temporal scales are poorly understood, and there is disagreement over which conservation strategies should be prioritized. We used 160 years of historic post‐agricultural woodland creation as a natural experiment to evaluate biodiversity responses to habitat creation in a landscape context. Birds were surveyed in 101 secondary, broadleaf woodlands aged 10–160 years with ≥80% canopy cover and in landscapes with 0‐17% broadleaf woodland cover within 3000 m. We used piecewise structural equation modeling to examine the direct and indirect relationships between bird abundance and diversity, ecological continuity, patch characteristics, and landscape structure and quantified the relative conservation value of local and landscape scales for bird communities. Ecological continuity indirectly affected overall bird abundance and species richness through its effects on stand structure, but had a weaker influence (effect size near 0) on the abundance and diversity of species most closely associated with woodland habitats. This was probably because woodlands were rapidly colonized by woodland generalists in ≤10 years (minimum patch age) but were on average too young (median 50 years) to be colonized by woodland specialists. Local patch characteristics were relatively more important than landscape characteristics for bird communities. Based on our results, biodiversity responses to habitat creation depended on local‐ and landscape‐scale factors that interacted across time and space. We suggest that there is a need for further studies that focus on habitat creation in a landscape context and that knowledge gained from studies of habitat fragmentation and loss should be used to inform habitat creation with caution because the outcomes are not necessarily reciprocal.     

Determinants of habitat association in a sympatric clade of marine fishes

Triplefin fishes reach their greatest diversity in New Zealand with 26 endemic species, and habitat diversification has been implicated as a key factor in the divergence of this group. Despite this, it is unknown whether species-specific habitat patterns in these sympatric fishes are established by passive processes (e.g. differential mortality) or by habitat selection during settlement. We investigate this question by comparing the habitat associations of new recruits with those of conspecific adults in five species. In addition, the amount of variation in habitat use of conspecific recruits and adults was calculated to identify ontogenetic shifts in habitat association. The results indicated that while there were some differences between recruit and adult habitats, these differences were small in magnitude and habitat use of new recruits was similar to that of adult conspecifics. This finding was further supported by the small difference in variation of habitat use between conspecific recruits and adults. The study suggests that new recruits are actively involved in the selection of habitats at settlement and maintain the use of these throughout demersal life. Habitat use in these territorial species has a large influence on mate choice, thus habitat selection by new recruits would provide a powerful mechanism for pre-zygotic isolation between individuals with different habitat preferences. Together these findings support the notion that habitat diversification has been a major component in the radiation of this sympatric group.     

Songbird abundance and parasitism differ between urban and rural shrublands.

Many studies have examined differences in avian community composition between urban and rural habitats, but few, if any, have looked at nesting success of urban shrubland birds in a replicated fashion while controlling for habitat. We tested factors affecting nest survival, parasitism by the Brown-headed Cowbird (Molothrus ater), and species abundance in shrubland habitat in rural and urban landscapes. We found no support for our hypothesis that nest survival was lower in urban landscapes, but strong support for the hypothesis that survival increased with nest height. We found strong support for our hypothesis that cowbird parasitism was greater in urban than rural landscapes; parasitism in urban sites was at least twice that of rural sites. We found strong support for an urban landscape effect on abundance for several species; Northern Cardinal (Cardinalis cardinalis) and Brown-headed Cowbirds were more abundant in urban landscapes, whereas Field Sparrow (Spizella pusilla) and Blue-winged Warbler (Vermivora pinus) were more abundant in rural sites. There was support for lower abundances of Blue-gray Gnatcatcher (Polioptila caerulea) and Indigo Bunting (Passerina cyanea) with increased housing density. For six other species, edge and trail density or vegetation parameters best explained abundance. Lower abundances and greater parasitism in habitat patches in urban landscapes are evidence that, for some species, these urban landscapes do not fulfill the same role as comparable habitats in rural landscapes. Regional bird conservation planning and local habitat management in urban landscapes may need to consider these effects in efforts to sustain bird populations at regional and local scales.     

Predicting structural complexity of reefs and fish abundance using acoustic remote sensing (RoxAnn)

This study determined whether the acoustic roughness of Caribbean reef habitats is an accurate proxy for their topographic complexity and a significant predictor of their fish abundance. Fish abundance was measured in 25 sites along the forereef of Glovers Atoll (Belize). At each site, in situ rugosity (ISR) was estimated using the “chain and tape” method, and acoustic roughness (E1) was acquired using RoxAnn. The relationships between E1 and ISR, and between both E1 and ISR and the abundance of 17 common species and the presence of 10 uncommon species were tested. E1 was a significant predictor of the topographic complexity (r ² = 0.66), the abundance of 10 common species of surgeonfishes, pomacentrids, scarids, grunts and serranids and the presence of 4 uncommon species of pomacentrids and snappers. Small differences in E1 (i.e. ∆0.05–0.07) reflected in subtle but significant differences in fish abundance (~1 individual 200 m⁻² and 116 g 200 m⁻²) among sites. Although we required the use of IKONOS data to obtain a large number of echoes per site, future studies will be able to utilise RoxAnn data alone to detect spatial patterns in substrate complexity and fish abundance, provided that a minimum of 50 RoxAnn echoes are collected per site.     

Antipredator strategies of house finches: are urban habitats safe spots from predators even when humans are around?

Urbanization decreases species diversity, but it increases the abundance of certain species with high tolerance to human activities. The safe-habitat hypothesis explains this pattern through a decrease in the abundance of native predators, which reduces predation risk in urban habitats. However, this hypothesis does not consider the potential negative effects of human-associated disturbance (e.g., pedestrians, dogs, cats). Our goal was to assess the degree of perceived predation risk in house finches (Carpodacus mexicanus) through field studies and semi-natural experiments in areas with different levels of urbanization using multiple indicators of risk (flock size, flight initiation distance, vigilance, and foraging behavior). Field studies showed that house finches in more urbanized habitats had a greater tendency to flock with an increase in population density and flushed at larger distances than in less urbanized habitats. In the semi-natural experiment, we found that individuals spent a greater proportion of time in the refuge patch and increased the instantaneous pecking rate in the more urbanized habitat with pedestrians probably to compensate for the lower amount of foraging time. Vigilance parameters were influenced in different ways depending on habitat type and distance to flock mates. Our results suggest that house finches may perceive highly urbanized habitats as more dangerous, despite the lower number of native predators. This could be due to the presence of human activities, which could increase risk or modify the ability to detect predators. House finches seem to adapt to the urban environment through different behavioral strategies that minimize risk.     

Multiple predators indirectly alter community assembly across ecological boundaries

Models of habitat selection often assume that organisms choose habitats based on their intrinsic quality, regardless of the position of these habitats relative to low-quality habitats in the landscape. We created a habitat matrix in which high-quality (predator-free) aquatic habitat patches were positioned adjacent to (predator-associated) or isolated from (control) patches with single or two species of caged predators. After 16 days of colonization, larval insect abundance was reduced by 50% on average in both the predator and predator-associated treatments relative to isolated controls. Effects were largely similar among predator treatments despite variation in number of predator species, predator biomass, and whether predators were native or nonnative. Importantly, the strength of effects did not depend on whether predators were physically present. These results demonstrate that predator cues can cascade with equal strength across ecological boundaries, indirectly altering community assembly via habitat selection in intrinsically high-quality habitats.     

Estimation of Core Terrestrial Habitat for Stream-Breeding Salamanders and Delineation of Riparian Buffers for Protection of Biodiversity

Abstract:  Many species of wildlife depend on riparian habitats for various life-history functions (e.g., breeding, foraging, overwintering). Although this unique habitat is critical for many species, delineations of riparian zones and buffers for various taxa are lacking. Typically when buffer zones are determined to mitigate edge effects, they are based on criteria that protect aquatic resources alone and do not consider impacts to wildlife and other terrestrial resources. Using two different survey methods (area-constrained daytime searches and nighttime visual encounter searches), we estimated core terrestrial habitat and buffer widths for stream-breeding salamanders in southern Appalachian streams from May to August 2004. A core terrestrial habitat of 27.0 m encompassed 95% of the salamander assemblage (four species of stream plethodontids), and an additional 50 m (to buffer edge effects) yielded a total buffer of 77.0 m. When each species of the assemblage was analyzed separately, the maximum core terrestrial habitat needed for the Blue Ridge two-lined salamander ( Eurycea wilderae ), a dominant member and the farthest-ranging species from the stream, was 42.6 m. Thus, we recommend an overall buffer width of 92.6 m in southern Appalachian streams. To protect stream amphibians and other wildlife dependent on riparian areas, land managers and policy makers must consider conserving more than aquatic resources alone. Developing core terrestrial habitat estimates and buffer zone widths for wildlife populations is a critical first step in the conservation of many semiaquatic organisms and protecting biodiversity.     

Macrofaunal responses to structural complexity are mediated by environmental variability and surrounding habitats

Investigating the context that surrounds each habitat is crucial to understand local responses of assemblages of species to habitats. Here, I tested whether responses of benthic macroinvertebrates to the structural complexity of experimental habitats were mediated by the characteristics of their surrounding habitats (i.e. rockpools or emergent-rock surfaces). Each type of surrounding habitat provided particular biotic (e.g. algal growth) and abiotic (e.g. temperature, water movement) conditions that were expected to affect benthic assemblages. The results show that (1) composition of entire assemblages was affected by the matrix and type of habitat; (2) effects of the matrix on the number of species varied depending on the different types of habitats; (3) abundant species showed specific responses to type of habitat, independently of the matrix; and (4) relationships between numbers of species and two major environmental variables (i.e. micro-algal biomass and sediment) varied depending on the type of habitats and the surrounding matrix. Generally, these findings demonstrate that understanding the consequences of the spatial structure of these habitats is essential to advance our knowledge on patterns of abundance and distributions of functionally important species and ultimately the structure of intertidal assemblages.     

Spatial repartition and ontogenetic shifts in habitat use by coral reef fishes (Moorea,French Polynesia)

This study explores the extent to which ontogenetic habitat shifts modify spatial patterns of fish established at settlement in the Moorea Island lagoon (French Polynesia). The lagoon of Moorea Island was divided into 12 habitat zones (i.e. coral seascapes), which were distinct in terms of depth, wave exposure, and substratum composition. Eighty-two species of recently settled juveniles were recorded from March to June 2001. Visual censuses documented changes in the distribution of juveniles of each species over time among the 12 habitats. Two patterns of juvenile habitat use were found among species. Firstly, some species settled and remained in the same habitat until the adoption of the adult habitats (i.e. recruitment; e.g. Chaetodon citrinellus, Halichoeres hortulanus, Rhinecanthus aculeatus). Secondly, others settled to several habitats and then disappeared from some habitats through differential mortality and/or post-settlement movement (e.g. 65–70 mm size class for Ctenochaetus striatus, 40–45 mm size class for Epinephelus merra, 50–55 mm size class for Scarus sordidus). A comparison of the spatial distribution of juveniles to that of adults (61 species recorded at both stages) illustrated four patterns of subsequent recruitment in habitat use: (1) an increase in the number of habitats used during the adult stage (e.g. H. hortulanus, Mulloidichthys flavolineatus); (2) a decrease in the number of habitats adults used compared to recently settled juveniles (e.g. Chrysiptera leucopoma, Stethojulis bandanensis); (3) the use of different habitat types (e.g. Acanthurus triostegus, Caranx melampygus); and (4) no change in habitat use (e.g. Naso litturatus, Stegastes nigricans). Of the 20 most abundant species recorded in Moorea lagoon, 12 species modified the spatial patterns established at settlement by an ontogenetic habitat shift.Communicated by T. Ikeda, Hakodate     

Marine reserve design: optimal size, habitats, species affinities, diversity, and ocean microclimate.

The design of marine reserves is complex and fraught with uncertainty. However, protection of critical habitat is of paramount importance for reserve design. We present a case study as an example of a reserve design based on fine-scale habitats, the affinities of exploited species to these habitats, adult mobility, and the physical forcing affecting the dynamics of the habitats. These factors and their interaction are integrated in an algorithm that determines the optimal size and location of a marine reserve for a set of 20 exploited species within five different habitats inside a large kelp forest in southern California. The result is a reserve that encompasses approximately 42% of the kelp forest. Our approach differs fundamentally from many other marine reserve siting methods in which goals of area, diversity, or biomass are targeted a priori. Rather, our method was developed to determine how large a reserve must be within a specific area to protect a self-sustaining assemblage of exploited species. The algorithm is applicable across different ecosystems, spatial scales, and for any number of species. The result is a reserve in which habitat value is optimized for a predetermined set of exploited species against the area left open to exploitation. The importance of fine-scale habitat definitions for the exploited species off La Jolla is exemplified by the spatial pattern of habitats and the stability of these habitats within the kelp forest, both of which appear to be determined by ocean microclimate.     

Habitat and landscape characteristics underlying anuran community structure along an urban-rural gradient

Urbanization has been cited as an important factor in worldwide amphibian declines, and although recent work has illustrated the important influence of broad-scale ecological patterns and processes on amphibian populations, little is known about the factors structuring amphibian communities in urban landscapes. We therefore examined amphibian community responses to wetland habitat availability and landscape characteristics along an urban-rural gradient in central Iowa, USA, a region experiencing rapid suburban growth. We conducted call surveys at 61 wetlands to estimate anuran calling activity, and quantified wetland habitat structure and landscape context. We used canonical correspondence analysis (CCA) to examine patterns in anuran community structure and identify the most important variables associated with those patterns. Urban density at the landscape scale had a significant negative influence on overall anuran abundance and diversity. While every species exhibited a decrease in abundance with increasing urban density, this pattern was especially pronounced for species requiring post-breeding upland habitats. Anurans most affected by urbanization were those associated with short hydroperiods, early breeding activity, and substantial upland habitat use. We suggest that broad-scale landscape fragmentation is an important factor underlying anuran community structure in this region, possibly due to limitations on the accessibility of otherwise suitable habitat in fragmented urban landscapes. This study underscores the importance of a regional approach to amphibian conservation in urban and urbanizing areas; in fragmented landscapes, a network of interconnected wetland and upland habitats may be more likely to support a successful, diverse anuran community than will isolated sites.     

Functional Equivalency between Rice Fields and Seminatural Wetland Habitats

Abstract: Evaluating the potential for anthropogenic habitats to act as surrogates for the natural habitats they replace is a key issue in conservation biology. In California, flooded rice fields are used by numerous aquatic birds during winter. If this habitat functions similarly to more natural wetlands, increased flooding may help replace the extensive wetlands that occurred in the region prior to agricultural development. I tested whether food abundance, perceived predation threat, foraging performance, and the way in which birds allocate their time to different behaviors differed between flooded rice fields and seminatural wetlands for several species of aquatic bird. When appropriate, I also compared flooded and unflooded fields. Invertebrate densities did not differ among habitats. Seminatural wetlands had less rice grain but more seeds from other plants than the two rice habitats. The frequency with which predators passed over a feeding area was lower in flooded fields than in unflooded fields or seminatural wetlands. Most differences in feeding performance and time allocation among habitats were small and statistically insignificant. For some species, feeding efficiency was greater in seminatural wetlands than in flooded fields. Increasing attack rates and the amount of time spent feeding when in flooded fields, however, may allow birds to compensate for reduced efficiency. Multivariate analyses showed that group size, predation threat, time of day, date, and water depth often were associated with behaviors, but that these variables rarely accounted for habitat differences. Flooded fields apparently provide equivalent foraging habitat to seminatural wetlands and, because of reduced predation threat, may be a safer habitat for waterbirds. Thus, if managed appropriately, one of the world's dominant forms of agriculture can provide valuable waterbird habitat.     

A Framework for Conceptualizing Human Effects on Landscapes and Its Relevance to Management and Research Models

Abstract: The concept of habitat fragmentation is limited in its ability to describe the range of possible landscape configurations created by a variety of disturbances. This limitation is especially problematic in landscapes where human use of the habitat matrix occurs at multiple levels and where habitat modification may be a more important consideration than a simple binary classification of habitat versus nonhabitat. We propose a synthesizing scheme that places intact, variegated, fragmented, and relictual landscape states on a continuum, depending on the degree of habitat destruction. At a second level, the scheme considers the patterns of habitat modification that are imposed on remaining habitats. Management for conservation involves halting and sometimes reversing the trends of habitat destruction and modification. Conservation strategies will differ according to the state of alteration of the landscape, but all strategies include some consideration of the degree of modification of the matrix in determining habitat viability. It is convenient for biologists to assess landscape alteration state in terms of the persistence of large structural elements such as trees. Because animal species use habitats differently, however, they also experience the landscape differently. A landscape considered structurally fragmented by humans may be functionally variegated to other species. Therefore, it is necessary to consider the extent to which the entire landscape, including the matrix, is accessible and utilized by organisms with different spatial scales of resource use.     

Quantifying Habitat Requirements of Tree-Living Species in Fragmented Boreal Forests with Bayesian Methods

Abstract:  Quantitative conservation objectives require detailed consideration of the habitat requirements of target species. Tree-living bryophytes, lichens, and fungi are a critical and declining biodiversity component of boreal forests. To understand their requirements, Bayesian methods were used to analyze the relationships between the occurrence of individual species and habitat factors at the tree and the stand scale in a naturally fragmented boreal forest landscape. The importance of unexplained between-stand variation in occurrence of species was estimated, and the ability of derived models to predict species' occurrence was tested. The occurrence of species was affected by quality of individual trees. Furthermore, the relationships between occurrence of species at the tree level and size and shape of stands indicated edge effects, implying that some species were restricted to interior habitats of large, regular stands. Yet for the habitat factors studied, requirements of many species appeared similar. Species occurrence also varied between stands; most of the seemingly suitable trees in some stands were unoccupied. The models captured most variation in species occurrence at tree level. They also successfully accounted for between-stand variation in species occurrence, thus providing realistic simulations of stand-level occupancy of species. Important unexplained between-stand variation in species occurrence warns against a simplified view that only local habitat factors influence species' occurrence. Apparently, similar stands will host populations of different sizes due to historical, spatial, and stochastic factors. Thus, habitat suitability cannot be assessed simply by population sizes, and stands lacking a species may still provide suitable habitat and merit protection.     

Effects of macroalgal structural complexity on nearshore larval and post-larval crab composition

Larval and post-larval crab distribution was surveyed in three different habitats in Kachemak Bay, Alaska to determine temporal and spatial variability. Distribution varied temporally and spatially from June 2005 to September 2006. Nine sites of varying habitat complexity were surveyed monthly using scuba surveys and light traps to measure habitat variables and quantify crab zoeae and megalopae. A total of 10,016 crabs belonging to seven families were identified. Four species comprised the majority (97%) of the total crab assemblages and included Cancer oregonensis, Fabia subquadrata, Telmessus cheiragonus, and Pugettia gracilis. Peak abundances occurred in summer but varied on small temporal scales with species. No single bay-wide variable determined the appearance of all species. Depending on species, appearance may be influenced by seasonality of environmental variables. Spatially, highest abundances occurred in habitats with less structural complexity. Spatial differences in crab abundance may have resulted from variability on large scale physical transport mechanisms and not kelp-mediated flow alterations.     

Climate, habitat, and species interactions at different scales determine the structure of a Neotropical bat community

Climate, habitat, and species interactions are factors that control community properties (e.g., species richness, abundance) across various spatial scales. Usually, researchers study how a few properties are affected by one factor in isolation and at one scale. Hence, there are few multi-scale studies testing how multiple controlling factors simultaneously affect community properties at different scales. We ask whether climate, habitat structure, or insect resources at each of three spatial scales explains most of the variation in six community properties and which theory best explains the distribution of selected community properties across a rainfall gradient. We studied a Neotropical insectivorous bat ensemble in the Isthmus of Panama with acoustic monitoring techniques. Using climatological data, habitat surveys, and insect captures in a hierarchical sampling design we determined how much variation of the community properties was explained by the three factors employing two approaches for variance partitioning. Our results revealed that most of the variation in species richness, total abundance, and feeding activity occurred at the smallest spatial scale and was explained by habitat structure. In contrast, climate at large scales explained most of the variation in individual species' abundances. Although each species had an idiosyncratic response to the gradient, species richness peaked at intermediate levels of precipitation, whereas total abundance was very similar across sites, suggesting density compensation. All community properties responded in a different manner to the factor and scale under consideration.     

Estuarine Vegetated Habitats as Corridors for Predator Movements

Abstract: The spatial proximity of one habitat to another can strongly influence population and community dynamics. We investigated whether the proximity of intertidal oyster reefs to vegetated estuarine habitats, salt marshes, and seagrass beds, affects the abundance and community structure of benthic macroinvertebrates on reefs and predator-prey interactions between mobile predators and bivalves living on reefs. Benthic macroinvertebrate abundance was highest on reefs spatially separated from salt marshes. Macroinvertebrate species richness was highest on reefs separated from both salt marshes and seagrass beds. Comparisons of predation on juvenile bivalves transplanted to reefs for 7–12 days indicated that survivorship of clams was greatest on reefs spatially separated from both salt marshes and seagrass beds, whereas reef proximity to vegetated habitats did not affect the survivorship of oysters. The foraging behavior of blue crabs may explain patterns of macroinvertebrate abundance and clam survivorship among reefs with different proximity to vegetated habitats. In experiments conducted in 30-m² field enclosures, blue crabs had higher predation rates on hard clams transplanted onto artificial reefs adjacent to salt marshes or seagrass beds than on reefs separated from both habitats by unvegetated sand bottom. Thus, vegetated habitats appeared to act as corridors by facilitating the access of blue crabs to oyster reefs and enhancing the intensity of blue crab predation. Such an understanding of the effects of landscape characteristics of estuarine habitats on their value as habitats for estuarine organisms can be used to predict the consequences of habitat fragmentation on ecosystem function and to improve strategies for habitat and species conservation and restoration.     

Defining Critical Habitats of Threatened and Endemic Reef Fishes with a Multivariate Approach

Understanding critical habitats of threatened and endemic animals is essential for mitigating extinction risks, developing recovery plans, and siting reserves, but assessment methods are generally lacking. We evaluated critical habitats of 8 threatened or endemic fish species on coral and rocky reefs of subtropical eastern Australia, by measuring physical and substratum‐type variables of habitats at fish sightings. We used nonmetric and metric multidimensional scaling (nMDS, mMDS), Analysis of similarities (ANOSIM), similarity percentages analysis (SIMPER), permutational analysis of multivariate dispersions (PERMDISP), and other multivariate tools to distinguish critical habitats. Niche breadth was widest for 2 endemic wrasses, and reef inclination was important for several species, often found in relatively deep microhabitats. Critical habitats of mainland reef species included small caves or habitat‐forming hosts such as gorgonian corals and black coral trees. Hard corals appeared important for reef fishes at Lord Howe Island, and red algae for mainland reef fishes. A wide range of habitat variables are required to assess critical habitats owing to varied affinities of species to different habitat features. We advocate assessments of critical habitats matched to the spatial scale used by the animals and a combination of multivariate methods. Our multivariate approach furnishes a general template for assessing the critical habitats of species, understanding how these vary among species, and determining differences in the degree of habitat specificity. Definición de Hábitats Críticos para Peces Arrecifales Amenazados y Endémicos Mediante un Método Multivariado