Associations between metals and the blue mesogleal protein of Cassiopea xamachana

The blue mesogleal pigment of the symbiotic jellyfish, Cassiopea xamachana Bigelow, 1882, is composed of two subunits, a larger glycosylated (35 kDa) moiety and a non-glycosylated (30 kDa) variant in lower concentration. In solution, the subunits assemble in large complexes of at least 10⁶ kDa. The pigment, known as Cassio Blue, appears to mitigate excessive solar radiation while allowing the passage of the wavelengths optimal for photosynthesis by the numerous algal symbionts in the mesoglea of the jellyfish. The pigment is an abundant protein comprising about 6% of all animal protein in the whole jellyfish and about 33% of all animal protein in the oral appendages. The protein also contains a diverse array of metals, notably Ag, Ca, Cu, Fe, Mg, and Zn, with traces of others. Metal stoichiometry varies among isolates averaging about 1 mol of all metals, taken together, for each mole of the pigment. Given the broad array of metals present, the pigment may also serve another purpose, for example, as a metal reservoir or trap. Few other proteins are associated with such a spectrum of metals. In addition, the amino acid sequences of the pigment tryptic peptides have no reasonable matches in any of the sequence databases. Our findings, taken as a whole, suggest that the Cassio pigment is indeed unusual and is likely a representative of a novel category of proteins, the original member of which is rpulFKz1, a chromoprotein endowed with Frizzled and Kringle domains.     

Comparison of telomere length among different life cycle stages of the jellyfish Cassiopea andromeda

The polyp (scyphistoma) of the jellyfish Cassiopea andromeda reproduces asexually repeatedly, while the medusa, the sexually reproducing stage, exhibits a relatively shorter life span. As a first step to understand the mechanism behind the differences in the life spans of the polyp and medusa stages of the jellyfish, we compared the lengths of the telomere region of one targeted chromosome between the polyp and medusa stages using a modified single telomere length assay (STELA). The double-stranded regions of the telomeres were amplified by PCR, and the average length of the PCR products was estimated by densitometry analysis of the gel smear. Chromosomes within cells of the bell region of the medusa were characterized by longer telomeres than those of polyps, asexual propagules, or other regions of the medusa. This is the first study to estimate the telomere lengths of targeted chromosomes in a cnidarian and opens a way to understand the mechanism underlying different life spans of the polyp and medusa stages.     

Threat of heavy metal contamination in eight mangrove plants from the Futian mangrove forest,China

Mangrove plants play an important role in heavy metal maintenance in a mangrove ecosystem. To evaluate the characteristics of heavy metal contamination in the Futian mangrove forest, Shenzhen, China, eight heavy metals in mangrove sediments and plants were monitored, including essential elements such as Cu and Zn, and non-essential elements such as Cr, Ni, As, Cd, Pb and Hg. The results showed that the heavy metals exhibited the following scheme: Zn > As > Cu ≈ Cr > Pb > Ni > Cd ≈ Hg in sediment cores, among which Cd, As, Pb and Hg contents were nearly ten times higher than the background values. There was no significant difference in metal maintenance capability between native and exotic species. In mangrove plants’ leaves and stems, concentrations of Cu, Zn and As were higher than other heavy metals. The low bioconcentration factors for most heavy metals, except for Cr, implied the limited ability of heavy metal accumulation by the plants. Mangrove plants seem to develop some degree of tolerance to Cr. The factor analysis implies that anthropogenic influences have altered metal mobility and bioavailability.     

Predator-inducible changes in blue mussels from the predator-free Baltic Sea

Blue mussels, Mytilus spp., have inhabited the brackish Baltic Sea, an environment lacking predatory crabs and starfish, for several thousand years. In this paper we examined whether Baltic Mytilus that were transplanted to the North Sea showed predator-inducible plasticity like their "marine" conspecifics. Our experiments showed that native North Sea Mytilus changed their morphology when exposed to waterborne scents from shore-crabs and starfish. These predators induced different kinds of changes, with emphasis on shell thickness and adductor muscle size, respectively. Baltic Mytilus responded in a similar way to crab scents, whereas starfish scents had a relatively weak effect on the morphology. Crab and starfish scent induced strengthening of the byssal attachment in North Sea Mytilus, with crabs providing more stimulation than starfish. Baltic mussels also improved the byssal attachment when exposed to either of the predators, but the attachment strength, as well as the response to crabs, were relatively weaker than that of North Sea mussels. We conclude that inducible plasticity still is present in Baltic Mytilus, despite their recent evolution in a predator-free environment. There is probably no strong selective pressure against inducible plasticity, but it could also be maintained in the population by gene flow from Mytilus in the adjoining North Sea. The question whether Baltic Mytilus are M. edulis or M. trossulus may also be relevant for the present results.     

Ultrastructure and histochemistry of the integument of air-breathing polychaetes from mangrove swamps of sumatra

The integument of S.E. Asian air-breathing polychaetes exhibits a variable ultrastructure with regard to cuticle and epithelial cells. The cuticle varies in thickness, contents of regularly arranged collagen fibrils, and structure of the epicuticle. The basal portion of the epithelium is either of smooth outline, or consists of slender processes of the epidermal cells separated by wide intercellular spaces which are invaded by a loose system of glia cells and nerve fibres. Extracellular spaces within the epidermis of the gills of Dendronereides heteropoda are transformed into blood lacunae. The enzyme content of the epithelial cells is relatively low.Supported by the Deutsche Forschungsgemeinschaft (Sto 75/2, We 380/4).     

Hydrogels for the removal of the methylene blue dye from wastewater: a review

Environmental Chemistry Letters - Water pollution is a major environmental issue with the rapid development of industry. Therefore, advanced technologies and materials are needed to remove...     

A blue carotenoprotein from the marine sponge Suberites domuncula: Purification and properties

A blue carotenoprotein, extracted from the marine spongia Suberites domuncula has been purified. The apoprotein has a molecular weight of about 31,000; the chromophore is not astaxanthin but it is similar to a monohydroxy, mono-expoxycarotene. The amino acid composition of the protein is briefly discussed.     

Impact of heavy metals on bacterial communities from mangrove soils of the Mahanadi Delta (India)

This study aimed to assess soil nutrient status and heavy metal content and their impact on the predominant soil bacterial communities of mangroves of the Mahanadi Delta. Mangrove soil of the Mahanadi Delta is slightly acidic and the levels of soil nutrients such as carbon, nitrogen, phosphorous and potash vary with season and site. The seasonal average concentrations (μg/g) of various heavy metals were in the range: 14 810–63 370 (Fe), 2.8–32.6 (Cu), 13.4–55.7 (Ni), 1.8–7.9 (Cd), 16.6–54.7 (Pb), 24.4–132.5 (Zn) and 13.3–48.2 (Co). Among the different heavy metals analysed, Co, Cu and Cd were above their permissible limits, as prescribed by Indian Standards (Co=17 μg/g, Cu=30 μ g/g, Cd=3–6 μ g/g), indicating pollution in the mangrove soil. A viable plate count revealed the presence of different groups of bacteria in the mangrove soil, i.e. heterotrophs, free-living N₂ fixers, nitrifyers, denitrifyers, phosphate solubilisers, cellulose degraders and sulfur oxidisers. Principal component analysis performed using multivariate statistical methods showed a positive relationship between soil nutrients and microbial load. Whereas metal content such as Cu, Co and Ni showed a negative impact on some of the studied soil bacteria.     

Arsenic speciation in polychaetes (Annelida) and sediments from the intertidal mudflat of Sundarban mangrove wetland, India

This paper documents the concentration of total arsenic and individual arsenic species in four soft-bottom benthic polychaetes (Perenereis cultifera, Ganganereis sootai, Lumbrinereis notocirrata and Dendronereis arborifera) along with host sediments from Sundarban mangrove wetland, India. An additional six sites were considered exclusively for surface sediments for this purpose. Polychaetes were collected along with the host sediments and measured for their total arsenic content using inductively coupled plasma mass spectrometry. Arsenic concentrations in polychaete body tissues varied greatly, suggesting species-specific characteristics and inherent peculiarities in arsenic metabolism. Arsenic was generally present in polychaetes as arsenate (As^V ranges from 0.16 to 0.50 mg kg^?1) or arsenite (As^III ranges from 0.10 to 0.41 mg kg^?1) (30–53 % as inorganic As) and dimethylarsinic acid (DMA^V <1–25 %). Arsenobetaine (AB < 16 %), and PO₄-arsenoriboside (8–48 %) were also detected as minor constituents, whilst monomethylarsonic acid (MA^V) was not detected in any of the polychaetes. The highest total As (14.7 mg kg^?1 dry wt) was observed in the polychaete D. arborifera collected from the vicinity of a sewage outfall in which the majority of As was present as an uncharacterised compound (10.3 mg kg^?1 dry wt) eluted prior to AB. Host sediments ranged from 2.5 to 10.4 mg kg^?1 of total As. This work supports the importance of speciation analysis of As, because of the ubiquitous occurrence of this metalloid in the environment, and its variable toxicity depending on chemical form. It is also the first work to report the composition of As species in polychaetes from the Indian Sundarban wetlands.     

Geographic variation in allele frequency of the gamete recognition protein M7 lysin throughout a mosaic blue mussel hybrid zone

Divergence at gamete recognition loci is hypothesized to result in speciation in broadcast spawning invertebrates. Many gamete recognition loci evolve quickly and show patterns of positive selection, yet the advantage of divergence is rarely known. M7 lysin is a sperm protein in the Mytilus edulis species complex that shows evidence of adaptive evolution. This locus is polymorphic with two distinct clades within Mytilus galloprovincialis, one of which, the D clade, shows the strongest signal of positive selection. We tested whether the geographic patterns in allele frequency were consistent with the hypothesis that positive selection on D clade alleles (G _D ) was due to reinforcement. Populations of M. edulis showed little evidence of introgression of G _D alleles, but there was no consistent evidence of reproductive character displacement. The lack of consistent patterns expected of reinforcement suggests that another mechanism is likely responsible for the adaptive divergence of M7 lysin.     

Fate of petroleum hydrocarbons taken up from food and water by the blue crab Callinectes sapidus

Radiolabeled paraffinic and aromatic hydrocarbons, including benzopyrene, fluorene, naphthalene, methylnaphthalene, methylcholanthrene, hexadecane, heptadecane and dotriacontane, were taken up from food and water by the blue crab Callinectes sapidus. In 2 days, approximately 10% of the benzopyrene and fluorene were taken up from the water when their concentrations were 2.5 and 30 g/l, respectively. When given food with radiolabeled hydrocarbons, 2 to 10% of the hydrocarbons were assimilated by the carbs, with the remainder excreted. After uptake of hydrocarbon from water or food, a major pathway for the elimination of hydrocarbon and metabolites was through fecal material. All hydrocarbons used in the study were metabolized, with similar rates for both paraffinic and aromatic hydrocarbons. More than 50% of the radioactivity assimilated by the crabs was in the hepatopancreas, suggesting that the hepatopancreas was the site of hydrocarbon metabolism. Twenty-five days aftex exposure to radiolabeled hydrocarbons, radioactivity was found only in the hepatopancreas. The hepatopancreas contained highly polar hydrocarbon metabolites, including dihydroxy-compounds and their conjugates, while blood contained both monohydroxy-and dihydroxy-compounds. No evidence was found of storage of hydrocarbons by any of the crab tissues.     

Levels of heavy metals in blue whiting caught from the eastern Black Sea area of Turkey

This paper presents data on the concentrations of 5 metals, copper (Cu), cadmium (Cd), iron (Fe), zinc (Zn) and lead (Pb) in Blue Whiting sampled from the Eastern Black Sea coast of Turkey. The highest metal concentrations of Cu, Cd, Fe, Zn and Pb were recorded in Blue Whiting with the values of 2.71, 0.601, 14.137, 15.322 and 1.078 μg g^‐1 dry weight, respectively. On average the metal concentrations in Blue Whiting followed the order of Zn > Fe > Cu > Pb > Cd. Temporal differences of concentrations of these metals were significant (p < 0.05). Spatial fluctuations of Cu, Cd, Fe, Zn and Pb concentrations in Blue Whiting were also significant (p < 0.01). It was found that the concentrations of Cu, Cd, Fe, Zn and Pb in the muscle in Blue Whiting were below the limit of Public Health Regulation in Turkey.     

An unusual abbreviated larval life in the estuarine burrowing prawn Callianassa kraussi (Crustacea: Decapoda: Thalassinidea)

The larval stages of Callianassa kraussi Stebb. were previously unknown. Larvae were bred out in the laboratory and found to be incapable of swimming. There are two larval stages, lasting 3 to 5 days in sea water of 34 to 35 S at 20 °C. Observations under simulated field conditions in the laboratory, and in the field—where burrow casts were made using a polyester resin, indicated that the larvae metamorphose to the first post-larval stage in the parent burrow, and then burrow directly into the wall of the parent burrow.     

Effect of heavy metals (Hg and Zn) on the growth and phosphate solubilising activity in halophilic phosphobacteria isolated from Manakudi mangrove

The diversity of phosphobacteria in Manakudi mangrove ecosystem of Tamil Nadu was carried out in root and rhizosphere soil samples. The counts of phosphobacteria were found higher in root samples than in soil samples particularly in Hymenachene acutigluma. The abundance of phosphobacterial diversity in Manakudi mangrove showed high degree of positive correlation with the content of phosphate in rhizosphere soil of all the mangrove and associated plant species. Nine phosphobacterial species belonging to 7 genera were reported from Manakudi mangrove ecosystem. All the identified bacterial species are sensitive to both the heavy metals (mercury and zinc) in terms of growth and physiology even at lower concentrations. The content of protein and total sugars were increased by the higher concentrations of heavy metals whereas decreased trend was noticed in lower concentrations of heavy metals.     

Studies on portunid crabs from the Eastern Pacific. II. Significance of the unusual distribution of Euphylax dovii

Euphylax dovii Stimpson (Brachyura: Portunidae: Podophthalminae), a tropical Eastern Pacific swimming crab, has distinctive morphological adaptations for pelagic existence. Crabs in collections from the open ocean had a sex ratio approximating 1:1, with no crabs bearing eggs. Samples from the continental shelf of Colombia contained thousands of females, mostly ovigerous, but no males. Egg attachment has posed a major problem in the evolution of decapod crustaceans, and the two genera of portunid crabs thus far observed cannot attach eggs unless females can bury partly in soft sediments. This suggests that mated E. dovii females must migrate into shallow shelf waters to encounter sediments necessary for spawning. The high energetic cost of swimming while carrying eggs and the presence of abundant food for larvae are factors favoring residence of females in shelf waters until hatching is complete.     

Responses of an estuarine population of the blue mussel Mytilus edulis to heated water from a steam generating plant

An entire bed of the blue mussel Mytilus edulis, consisting of 5,000 individuals/m², died during June, 1971 in the effluent canal of a steam generating plant when the temperature increased above 27°C. Similarly, the population in the intake canal disappeared when temperatures rose above 27°C in August. Laboratory studies showed that M. edulis could not tolerate continuous temperatures above 27°C, and feeding stopped shortly after the mussels were exposed to 25°C. Histopathological studies indicated that the cause of death of this bivalve was associated with degeneration of the frontal and laterofrontal cilia of the columnar epithelium of the gill filaments. In conjuction with this, there was necrosis and sloughing of the epithelium of the intestinal diverticula. Extensive amoebocytic infiltration was noted in the byssogenous cavity, gill filaments and stomach wall.This paper represents part of a dissertation by the first author, submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, University of Rhode Island, January 1973.     

An ichthyotoxic protein in the defensive skin secretion of the Red Sea trunkfish Ostracion cubicus

By the aid of acetone precipitation and RP-HPLC column chromatography coupled to various bioassays of ichthyotoxicity and cytolysis, a new ichthyotoxin designated “boxin” was isolated from the defensive skin secretion of the Red Sea trunkfish Ostracion cubicus. Boxin is a stable, heat and proteolysis resistant protein of 18 kDa. Its protein nature was assessed by spectral analysis, strong proteolysis, amino acid analysis and amino acid sequence determination. Similar to pahutoxin (PHN), an organic cationic surfactant derived from the same secretion, boxin is not effective by injection, and its ichthyotoxicity is achieved only upon delivery to the surrounding water. The latter suggests that fish lethality is mediated by externally allocated target sites (receptors). Boxin, however, differs from PHN by (1) possessing a 30 times higher ichthyotoxicity (mole per mole) and (2) being devoid of PHN's capacity to permeabilize biological membranes. From an ecological point of view, it is noteworthy that polypeptides are very useful in fulfilling allomonal functions in the marine environment due to the high information content inherent in their structures and their solubility in seawater. Received: 22 September 1999 / Accepted: 28 January 2000     

Occurrence and characteristics of unusual protistan symbionts from surgeonfishes (Acanthuridae) of the Great Barrier Reef,Australia

The occurrence of unusual symbiotic microorganisms was examined in the intestines of a range of fish from the Great Barrier Reef, Australia. The fish taxa examined included 26 species of the family Acanthuridae, as well as representatives of phylogenetically related and herbivorous taxa. The microorganisms, referred to as protists, were only found in herbivorous and detritivorous members of the Acanthuridae. Protists were not found in planktivorous acanthurids, nor in any members of the families Kyphosidae, Pomacentridae, Scaridae, Zanclidae, Siganidae and Bleniidae we examined. In addition, protists were absent from the herbivorous acanthurids A. xanthopterus and A. nigricans. A range of protist forms, characterized by differences in size (8 to 417 m), shape and mode of cell division (daughter-cell production and binary fission), was observed. The occurrence of these forms appeared to be correlated with host feedingecology. Large forms (>100 m) of the protists were only found in acanthurids which fed over hard-reef substrata. Smaller forms were found in sand-grazing and detritivorous species. One of the protist forms appears identical to protists previously reported from Red Sea acanthurids.     

A comparison of Lowry,Bradford and Smith protein assays using different protein standards and protein isolated from the marine diatom Thalassiosira pseudonana

Two spectrophotometric assays for protein commonly used in marine research (Coomassie stain, Bradford; alkaline copper, Lowry) and a more recent assay which has not been applied in this field (bicinchoninic acid, Smith) were compared for homogenates of the marine diatom Thalassiosira pseudonona using bovine serum albumin (BSA) as a standard. When homogenates were prepared by precipitating protein with trichloroacetic acid (TCA) and redissolving in 1 N NaOH, the protein content estimated by the Lowry and Smith assays agreed closely, but was consistently 20% higher than that indicated by the Bradford assay. To determine if this difference was due to the choice of a protein standard, protein from T. pseudonana was purified and compared to BSA, bovine gamma-globulin (BGG), and casein. The reactivity of the purified protein (expressed as the slope of the absorbance vs protein concentration curve) did not differ between cultures grown at high or low irradiance. For the Smith and Bradford assays the reactivity of BSA was not significantly different from algal protein, but for the Lowry assay, algal protein was significantly higher in reactivity than BSA. BGG was not significantly different in reactivity from algal protein for the Lowry and Smith assays, but BGG gave significantly lower absorbances than algal protein in the Bradford assay. These results suggest that BSA is a suitable standard for algal protein in the Bradford assays, while BGG is preferable for the Lowry assay. Either protein standard could be used for the Smith assay. Differences in purified algal protein reactivity compared to BSA could not account for the differences among the assays, nor could interference by chlorophyll a. Precipitating protein with TCA prior to analyses gave lower protein than direct analyses of homogenates for the Lowry and Smith assays, but no differences were found for the Bradford assay. As a result, the Lowry and Smith assays indicated up to 60% greater protein than the Bradford if TCA precipitation was not performed. This may be due to removal of free amino acids and small peptides which are less reactive in the Bradford assay. The 20% higher protein found in the Lowry or Smith vs Bradford assays may be due to different assay sensitivity to small peptides or other compounds which are precipitated along with proteins by TCA. Although the Smith assay is substantially simpler to perform than the Lowry, there appear to be no quantitative differences in the results. It remains unclear which spectrophotometric assay is most accurate, but the Bradford assay is faster and simpler, and is less likely to be affected by non-protein compounds found in marine phytoplankton.     

Cytology of an unusual case of hermaphroditic gonads in the tropical sea urchin Tripneustes gratila from Eilat (Red Sea)

One specimen of the tropical sea urchin Tripneustes gratila, distributed along the rocky subtide of Eilat, Israel, was found to be hermaphroditic, producing fertile sperm and eggs. In this case, internal self-fertilization, producing embryos along the gonads, was also observed.