共查询到20条相似文献,搜索用时 31 毫秒
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Andrew B. Sparks Eric T. Wang Craig A. Struble Wade Barrett Renee Stokowski Celeste McBride Jacob Zahn Kevin Lee Naiping Shen Jigna Doshi Michel Sun Jill Garrison Jay Sandler Desiree Hollemon Patrick Pattee Aoy Tomita-Mitchell Michael Mitchell John Stuelpnagel Ken Song Arnold Oliphant 《黑龙江环境通报》2012,32(1):3-9
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Yun-Ling Zheng Maryann Demaria Dongkai Zhen Theresa J. Vadnais Diana W. Bianchi MD 《黑龙江环境通报》1995,15(10):897-905
Monoclonal antibody to fetal haemoglobin (a2γy2) has been proposed as a fetal-specific reagent. We developed an intracellular staining protocol that combines fluorescein isothiocyanate or phycoerythrin conjugated anti-γ with the DNA binding dye Hoechst 33342 to identify and flow sort fetal erythroblasts from maternal blood. Our preliminary observations on anti-γ-positive cells sorted from four different pregnant women are described here, using fluorescence in situ hybridization (FISH) with chromosome-specific probes to identify fetal cells. Our data demonstrate that far fewer candidate fetal cells are sorted with this protocol than by current cell surface staining methods that employ the monoclonal antibody CD71. This results in increased fetal cell sorting purities. With this protocol, standard FISH techniques require modification due to the rigorous fixation with 4 per cent paraformaldehyde. Our initial data indicate the promise of this approach. 相似文献
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