Introduction to and experimental conditions during the first year of the GMR chronic inhalation studies of diesel exhaust

A chronic exposure study was initiated to determine the effects of diesel exhaust on the health of experimental animals. For this purpose, test atmospheres of clean air (control) or freshly diluted diesel exhaust at concentrations of 250, 750, and 1500 μg/m³ were supplied to four 12.6 m³ inhalation chambers which housed rats and guinea pigs. Diesel aerosol size and concentration, as well as chamber temperature and relative humidity, were continually monitored and controlled to maintain the exposure dose levels and an environment of 22±2°C and 50%±20% relative humidity. The concentrations of CO and NO_x were found to be 5.8±1.0 mg/m³ and 7.9±1.0 mg/m³ above ambient in the chamber containing 1500 μg/m³ of particulate. Animals were supplied from the chambers, on a random basis, for both intramural and extramural studies throughout the exposure period. The experiment ran uninterrupted for over twelve months with mean diesel particle mass concentrations within 2% of the target values.     

Genotoxicity of syrian hamster lung cells treated in vivo with diesel exhaust particulates

Polycyclic aromatic hydrocarbons extracted and concentrated from diesel exhaust particulates have been shown to be mutagenic and carcinogenic, but attempts to induce pulmonary tumors through chronic inhalation of diesel exhaust by experimental animals have failed. We have attempted to resolve this incongruity by measuring chromosomal damage in lung tissue of chronically exposed hamsters, using the highly sensitive test for genotoxic chemical agents, sister chromatid exchange (SCE) analysis. To determine the degree of responsiveness of the test system to both diesel exhaust particulates and benzo(a)pyrene (BaP), these agents were instilled intratracheally into anesthetized hamsters as suspensions in 0.25 ml volumes of Hank's balanced salt solution (HBSS). Lung tissues from these animals were subsequently cultured in vitro and chromosomes from the resulting cell divisions were scored for exchanges of chromatin between sister chromatids. Control animals, treated weekly with 0.25 ml of BSS for 10 weeks, showed an average value of 12 SCE's per cell, while animals treated weekly with 200 ng BaP over a 10-week period showed an average of 17 SCE's per cell. HBSS, given as a single treatment, also produced an average of 12 SCE's per cell in control animals, but animals treated with a single instillation of 12.5 μg BaP showed an average SCE value of 19. These data confirmed that the procarcinogen BaP can be metabolically activated by lung cells in vivo and also demonstrated the efficacy of using this technical approach to study the effect of chemical mutagens that enter the lungs. Diesel exhaust particulates, administered in a range from 0 to 20 mg per hamster over a 24 h exposure period, produced a linear SCE dose-response ranging from 12 to 26 SCE's per metaphase. This curve suggested that a concentration of 3 mg of diesel particulates per hamster would not produce a statistically significant increase in SCE's above control values. One group of 8 hamsters, chronically exposed to diesel exhaust particulates for 3 months showed an average of 12 SCE's per cell. This was equivalent to a set of 5 control animals which also showed an average of 12 SCE's per cell. Although the scope of this study was limited, the data demonstrated that diesel exhaust particulates can induce genotoxic damage but a 3-month exposure to 6 mg/m³ of diesel exhaust particulates was insufficient to produce measurable mutagenic changes in lung cells. This negative response is consistent with the results from other studies in which similar exposures failed to produce pulmonary tumors.     

In vivo detection of mutagenic effects of diesel exhaust by short-term mammalian bioassays

Male Chinese hamsters were exposed to diesel exhaust and clean air for six months at the Center Hill Facility of the U.S. Environmental Protection Agency in Cincinnati, Ohio. The animals were kept in specially constructed inhalation chambers and exposed to clean air or diesel exhaust for eight hours daily. The animals were sacrificed and slides prepared to study the mutagenic effects of diesel exhaust by four in vivo short term mammalian bioassays. Sperm morphology bioassay revealed a 2.67-fold increase in sperm abnormalities in the animals exposed to diesel exhaust as compared to those exposed to fresh air. Micronucleus bioassay revealed a 50% increase in the number of micronuclei in polychromatic erythrocytes obtained from animals exposed to diesel exhuast. However, no increase in sister chromatid exchange or chromosomal abnormalities was observed in bone marrow cells of animals treated with diesel exhaust. During these studies a decrease in mitotic index was observed in animals treated with diesel exhaust.     

Pulmonary function testing of animals chronically exposed to diluted diesel exhaust for 267 days

Thw purpose of this work was to assess the potential effect that chronic inhalation of diesel exhaust may have on lung mechanics and lung volumes. Noninvasive pulmonary function tests have been conducted repeatedly on 25 rats exposed to diesel exhaust at a particulate concentration of 1500 μg/m³, for 20 h/day, days/week for 267 days. The same tests were conducted on 25 clean air control animals. When the data is normalized, there are no apparent functionally significant changes occurring in the lungs that may be attributed to the chronic inhalation of diesel exhaust.     

Farmworker children's residential non-dietary exposure estimates from micro-level activity time series

Farmworkers' children may have increased pesticide exposure through dermal absorption and non-dietary ingestion, routes that are difficult to measure and model. The Cumulative Aggregate Simulation of Exposure (CASE) model, integrates the complexity of human behavior and variability of exposure processes by combining micro-level activity time series (MLATS) and mechanistic exposure equations. CASE was used to estimate residential non-dietary organophosphate pesticide exposure (i.e., inhalation, dermal, and non-dietary ingestion) to California farmworker children and evaluate the micro-activity approach. MLATS collected from children and distributions developed from pesticide measurements in farmworkers' residences served as inputs. While estimated diazinon exposure was greater for inhalation, chlorpyrifos exposure was greater for the other routes. Greater variability existed between children (σ_B² = 0.22–0.39) than within each child's simulations (σ_W² = 0.01–0.02) for dermal and non-dietary ingestion. Dermal exposure simulations were not significantly different than measured values from dosimeters worn by the children. Non-dietary ingestion exposure estimates were comparable to duplicate diet measurements, indicating this route may contribute substantially to aggregate exposure. The results suggest the importance of the micro-activity approach for estimating non-dietary exposure. Other methods may underestimate exposure via these routes. Model simulations can be used to identify at-risk children and target intervention strategies.     

Population exposure to airborne thorium at the high natural radiation areas in India

High natural radiation areas in the coastal and peninsular India were studied for airborne thorium and resultant population exposure due to inhalation. Four locations covering three states viz., Ayiramthengu and Neendakara in Kerala, Kudiraimozhi in Tamil Nadu and Bhimilipatnam in Andhra Pradesh were investigated. External gamma radiation fields 1 m above the monazite ore bodies ranged from 200 to 3000 nGy h^-1. Soil samples showed ²³²Th specific activity varying from 0·1 to 1·5 Bq g^-1 with surface alpha activity in the range of 1·0–12·5 Bq cm^-2. Suspended particulates in the samples ranged from 60–140 μg m^-3 with ²³²Th showing a wider variation of <0·03–0·3 mBq m^-3. There was poor correlation between suspended particulates and long-lived alpha airborne activity . The resuspension factors for ²³²Th were in the range of 1·5×10^-8–7·9×10^-7 cm^-1. Higher resuspension was correlated with dry sand dunes. The upper limits for Committed Effective Dose (CED) due to inhalation of airborne ²³²Th at the respective high natural radiation areas were estimated to range from 50±30 to 300±130 μSv (5–30 mrem) per year per adult member of public assuming an activity median aerodynamic diameter of 1 μm for the airborne particulates.     

Abnormal Cranial Variation in the Sable (Martes zibellinaL.) and the State of Environment

An abnormal cranial variation in the sable (Martes zibellinaL.) from the extreme Russian Northeast was studied. In the populations formed as a result of reacclimation, the proportion of animals with morphological cranial anomalies proved to be significantly higher than in the parental populations (from which the animals for reintroduction were taken). Probable factors responsible for this effect are discussed.     

Spatial Distribution of Sables in the Middle Ob Region

Results of stationary studies on the spatial location of sables (Martes zibellinaL.) belonging to different age and sex groups are summarized. Mapping the sites where 388 animals were taken, it was found that young animals tended to live near floodplains; adult animals, on watersheds. Analysis of sables taken by commercial hunters (n= 1833) made it possible to estimate the age composition and the degree and spatial distribution of the hunting pressure on sable populations in three areas of the Ob region. The spatial separation of young and reproductive animals is interpreted as a group adaptive response to the specific distribution pattern of different habitats typical of this species and to the pattern of animal elimination. It is proposed to use the specific pattern of animal distribution for maintaining the size of the population that is subject to hunting. Specifically, reserve (reproductive) territories should be organized in watershed areas.     

Structure of fouling communities formed by <Emphasis Type="Italic">Halichondria panicea</Emphasis> (Porifera: Demospongiae) in the White Sea

The structure of fouling communities formed by the sponge Halichondria panicea in the White Sea is described. On artificial substrates exposed in the sea for a long time, this sponge forms overgrowths on the colonies of long-lived organisms such as the mussel Mytilus edulis or the ascidian Styela rustica. The biomass of algae and sedentary animals (other than H. panicea) on the substrate is markedly smaller in areas colonized by this sponge than in neighboring sponge-free areas. Conversely, the biomass of both sedentary and errant polychaetes is greater in H. panicea colonies than in adjacent fouling communities.     

Pulmonary function changes in Chinese hamsters exposed six months to diesel exhaust

Chinese hamsters were exposed for eight hours per day to automotive diesel exhaust emissions which were diluted with air (18 to 1) and had a particulate level of 6.4 mg/m³. Pulmonary function measurements were made after six months exposure. Body weight (BW), lung weight (LW), vital capacity (VC), residual volume by water displacement (RV_w) and by gas dilution (RV_D), alveolar volume (V_A), and carbon monoxide transfer factor (D_LCO) were measured. LW showed a significant increase in the diesel exposed animals (P < 0.01) while VC, RV_W, and D_LCO showed decreases (P < 0.01). Static deflation volume-pressure curves showed depressed deflation volumes for diesel exposed animals when volumes were corrected for body weight and even greater depressed volumes when volumes were corrected for lung weight. However, when volumes were expressed as percent vital capacity, the diesel exposed animals had higher lung volumes at 0 and 5 cm H₂O. Results of the pathological examination of the lung tissue will be necessary for final analysis of our findings. However, preliminary interpretation indicates possible emphysematous changes which are compatible with the observed decrease in D_LCO.     

Nationwide indoor 222Rn and 220Rn map for India: a review

Considering the role of radon in epidemiology, an attempt was made to make a nation-wide map of indoor ²²²Rn and ²²⁰Rn for India. More than 5000 measurements have been carried out in 1500 dwellings across the country comprising urban and nonurban locations. The solid state nuclear track detectors based twin cup ²²²Rn/²²⁰Rn discrimination dosimeters were deployed for the measurement of indoor ²²²Rn, ²²⁰Rn and their progeny levels. The geometric means of estimated annual inhalation dose rate due to indoor ²²²Rn, ²²⁰Rn and their progeny in the dwellings was 0.94 mSvy⁻¹ (geometric standard deviation 2.5). It was observed that the major contribution to the indoor inhalation dose was due to indoor ²²²Rn and its progeny. However, the contribution due to indoor ²²⁰Rn and its progeny was not trivial as it was found to be about 20% of the total indoor inhalation dose rates. The indoor ²²²Rn levels in dwellings was significantly different depending on the nature of walls and floorings.     

Intrapopulation variation in the quality of dandelion seed progeny in zones of chemical and radioactive contamination

Interfamilial variation in metal tolerance and radioresistance of seed progeny from F ₁ plants was studied in the dandelion from cenopopulations growing over several decades in gradients of radioactive or chemical contamination. The progeny from the impact zone of the Eastern Ural Radioactive Trace (EURT) proved to be highly viable but had latent injuries that accounted for a high sensitivity to additional technogenic impact. In the F ₁ generation from the zone of chemical contamination, high seed viability was combined with increased tolerance to additional impact of radiation and heavy metals. No significant differences between responses to challenge with habitual and new factors were revealed in samples from either zone.     

Exposure to inhaled THM: Comparison of continuous and event-specific exposure assessment for epidemiologic purposes

Trihalomethanes (THMs) (chloroform, bromoform, dibromochloromethane, and bromodichloromethane) are the most abundant by-products of chlorination. People are exposed to THMs through ingestion, dermal contact and inhalation. The objective of this study was to compare two methods for assessing THM inhalation: a direct method with personal monitors assessing continuous exposure and an indirect one with microenvironmental sampling and collection of time–activity data during the main event exposures: bathing, showering and swimming. This comparison was conducted to help plan a future epidemiologic study of the effects of THMs on the upper airways of children. 30 children aged from 4 to 10 years were included. They wore a 3M^™ 3520 organic vapor monitor for 7 days. We sampled air in their bathrooms (during baths or showers) and in the indoor swimming pools they visited and recorded their time–activity patterns. We used stainless steel tubes full of Tenax^® to collect air samples. All analyses were performed with Gas Chromatography and Mass Spectrometry (GC-MS). Chloroform was the THM with the highest concentrations in the air of both bathrooms and indoor swimming pools. Its continuous and event exposure measurements were significantly correlated (r_s = 0.69 p < 0.001). Continuous exposures were higher than event exposures, suggesting that the event exposure method does not take into account some influential microenvironments. In an epidemiologic study, this might lead to random exposure misclassification, thus underestimation of the risk, and reduced statistical power. The continuous exposure method was difficult to implement because of its poor acceptability and the fragility of the personal monitors. These two points may also reduce the statistical power of an epidemiologic study. It would be useful to test the advantages and disadvantages of a second sample in the home or of modeling the baseline concentration of THM in the home to improve the event exposure method.     

Temperature adaptation of the heart in the grass snake (Natrix natrix L.), common European viper (Vipera berus L.), and steppe viper (Vipera renardi Christoph) (Reptilia: Squamata: Serpentes)

Changes in the heart rate (HR) in more and less thermophilic reptiles (Natrix natrix L., Vipera berus L., and V. renardi Christoph) exposed to heating from 0 to 40°C under anesthesia have been found to depend on the previous adaptation of the animals to the temperature parameters characteristic of the natural habitats of their populations.     

Potential source of asbestos in non-asbestos textile manufacturing company

Recently, a worker with lung carcinoma and a metastatic brain tumor was diagnosed as having a work-related disease. He had been employed in a non-asbestos textile company for 25 years. Consequently, to identify and explore possible causative agents for lung cancer in a non-asbestos textile manufacturing company and establish a causal relationship between exposure and lung cancer, an epidemiological investigative study was conducted and the work processes the worker was engaged in were examined. Air samples were taken from the workplace and during the drilling processes, and a suspected causative material was analyzed. The study revealed that the subject had been employed in the non-asbestos textile manufacturing company for 25 years from 1973 and his responsibilities included repairing spinning machines. In particular, the subject was involved in drilling B-bushings that were used to protect against gear abrasion in the spinning machines. An analysis of the B-bushings using a transmission electron microscope equipped with an energy dispersive X-ray analyzer indicated that they contained crocidolite asbestos fibers. Air samples obtained when drilling the B-bushings clearly indicated that the subject had most likely been exposed to crocidolite fibers when installing the B-bushings in the spinning machines. The frequency and duration of the work suggested that there would be a sufficient degree of exposure to crocidolite fibers to cause lung cancer. Except for smoking and asbestos exposure, no other chemical exposure was suspected for developing lung cancer in the workplace. Smoking appeared to be more of a potentiating risk factor in conjunction with the asbestos exposure. Accordingly, this case may provide significant evidence in identifying the cause of the mesothelioma or lung carcinoma found among workers in non-asbestos textile manufacturing companies elsewhere.     

“Not All Environmentalists Are Like That … ”: Unpacking the Negative and Positive Beliefs and Perceptions of Environmentalists

ABSTRACT

Limited research to date has qualitatively explored the perceptions members of the public who are not environmental activists hold of environmentalists. Therefore a qualitative survey was conducted with 89 US residents aged 21–53 (M_age?=?32.74, SD_age?=?7.89) to obtain an in-depth understanding of how non-activists within the public perceive environmentalists. Data obtained were analyzed using thematic analysis and demonstrated that non-activist perceptions of environmentalists contained both positive and negative components. Environmentalists were seen to value nature and to be actively involved in bringing about positive environmental change (positive component), yet were also viewed as aggressive in their behaviors and stubborn in their beliefs (negative component). Further still, it was found that environmentalists were more likely to be perceived positively when they engaged in individual-level, private sphere behaviors (such as recycling), and negatively when they engaged in collective-level, public sphere behaviors (such as protesting). These findings not only challenge the assumption that members of the public typically evaluate environmentalists negatively, they also outline why some individuals may fail to identify as an environmentalist and engage in pro-environmental behavior. Furthermore, they also provide some insight as to why some environmentalists find it difficult to advocate for system change that results from collective action within the public sphere.     

Airborne radioactivity on the Scottish Solway coast

A brief survey of the concentrations of radionuclides in surface air at five locations along the Scottish shoreline of the Irish Sea has been made in response to concern that airborne resuspension of marine radioactivity may be causing an inhalation hazard. Airborne concentrations of ⁴⁰K, ¹⁰⁶Ru, ¹³⁷Cs, ¹⁴⁴Ce, ²³⁸Pu, ^{239 + 240}Pu and ²⁴¹Am are reported. Plutonium isotopic ratios indicate the presence of radioactivity of marine origin attributable to low-level waste disposals from the fuel-reprocessing plant at Sellafield in Cumbria. Calculation of the dose resulting from inhalation shows that the risks to members of the public are negligible. Suggestions are made for development of these studies in future.     

Noninvasive molecular genetic analysis in studying the ecology of the snow leopard: Problems and prospects

Molecular genetic methods were used to perform the species and individual identification of 117 samples of hair and feces of carnivores collected in natural habitats. The species were identified for 85.5% of the samples. The analysis of the nucleotide sequences of the cytochrome b mitochondrial gene was used to identify the samples of hair and feces of snow leopards (Uncia uncia, 40%), red foxes (Vulpes vulpes, 48%), wolves (Canis lupus, 6%), and lynx (Lynx lynx, 5%). The analysis of eight microsatellite loci allowed the genotypes of 13 snow leopards to be described. The sex of 11 animals was identified.     

Reframing Ecotage as Ecoterrorism: News and the Discourse of Fear

This study examines how certain Western institutional discourses reproduce particular human relationships with nature. The analysis focuses upon the institutional setting of the zoo, examining long-standing multi-voiced debates about zoos and exploring the contemporary zoo's conservation discourses and cultural, lexical, and spatial elements of gaze and power. The author contextualizes zoo discourses within Western ideological environmental dialectics, including those of Mastery–Harmony, Othering–Connection, and Exploitation–Idealism. The author relates these discussions to her empirical observations of how certain discursive themes are reproduced and complicated within a leading American zoo. In the tradition of critical research that advocates for social change, the essay concludes with analysis-driven discussion about possibilities for zoos to transform their core configurations to more progressively work as agents for systemic cultural and environmental change.     

Coat color polymorphism in populations of the northern mole vole in the Ural Region

Collections made in the course of long-term field studies on ecology of the northern mole vole Ellobius talpinus Pall. in the Ural Region and neighboring areas (more than 2000 individuals from 24 points of the species range) were used to analyze geographic variation in its coat color (color morphs). On the basis of long-term observations (1985–1999) on marked animals from a polymorphic population (Kurtamyshskii raion, Kurgan oblast), the life spans of males and females and the dependence of life span on population density and structure were estimated in animals of different color morphs. Each color morph of E. talpinus was shown to have specific features of the seasonal dynamics of age structure and migrations.