共查询到20条相似文献,搜索用时 31 毫秒
1.
Summary. Antennae of six sympatric bark and ambrosia beetles (Scolytidae), Dendroctonus valens LeConte, Gnathotrichus retusus (LeConte), Hylastes tenuis Eichhoff, Ips mexicanus (Hopkins), Ips plastographus maritimus Lanier, and Pseudohylesinus sericeus (Mannerheim), and two scolytid predators, Enoclerus sphegeus (F.) (Cleridae) and Lascontonus tuberculatus Kraus (Colydiidae), were analyzed by gas chromatographic-electroantennographic detection (GC-EAD) for their responses to
synthetic Ips spp. pheromone components, and host and nonhost volatiles.
The beetles emerged from cut logs of pitch canker-infected Monterey pine trees, Pinus radiata D. Don. There were significant disparities in EAD response patterns to the hemiterpene and monoterpene alcohol pheromone
components that are typically produced by Ips spp. Antennae of I. p. maritimus responded strongly to ( ± )-ipsdienol, ( ± )-ipsenol, amitinol, and lanierone; antennae of I. mexicanus responded strongly to (1S,2S)-(–)-cis-verbenol, with weaker responses to ( ± )-ipsdienol, ( ± )-ipsenol, and amitinol; antennae of H. tenuis responded to (1S, 2R)-(–)-trans-ver-benol, with less pronounced responses to (–)-cis-verbenol and 2-methyl-3-buten-2-ol; and antennae of D. valens, G. retusus, and P. sericeus generally responded to all Ips spp. pheromone components except 2-methyl-3-buten-2-ol (D. valens and G. retusus) and E-myrcenol (G. retusus and P. sericeus). Ips mexicanus
responded only to the (–)-enantiomers of ipsenol and ipsdienol, whereas I. p. maritimus responded to (–)-ipsenol, but to both the (+)- and (–)-enantiomers of ipsdienol. The antennae of the two predaceous insects
(E. sphegeus and L. tuberculatus) responded to a range of the Ips spp. pheromone components. Host monoterpenes elicited no antennal responses from E. sphegeus, G. retusus, H. tenuis, and I. mexicanus, but several monoterpenes elicited various levels of responses from D. valens and I. p. maritimus antennae. Interestingly, antennae of female D. valens responded to (–), but not (+)-limonene. α- and β-Pinene elicited weak responses from L. tuberculatus antennae. EAD responses to selected nonhost volatiles were almost identical among the six scolytid species, with trans-conophthorin eliciting the strongest response in most cases, followed by three C6- alcohols and two C8-alcohols. The antennal responses by most of these species to linalool or geranylacetone were very weak; (E)-2-hexenal, (Z)-3-hexenyl acetate, and benzyl alcohol elicited almost no response. The response pattern of P. sericeus to nonhost volatiles differed slightly from the rest of the scolytids: a strong response to linalool, weaker response to
the C8-alcohols. The two predaceous Coleoptera generally had weak, but detectable, responses to nonhost volatiles, except for a
relatively strong response to trans-conophthorin by L. tuberculatus. No notable differences in EAD responses were observed between males and females of the two Ips spp. Our results provide an electrophysiological baseline for future efforts to identify attractive and repellent semiochemicals
(aggregation pheromones, host kairomones, or nonhost interruptants) for this guild of scolytids and their key predators that
are associated with moribund and pitch canker- infected P. radiata. 相似文献
2.
M. Subchev T. Toshova C. Koshio S. Franke A. Tröger R. Twele W. Francke J. A. Pickett L. J. Wadhams C. M. Woodcock 《Chemoecology》2009,19(1):47-54
The plum moth, Illiberis rotundata Jordan (Lepidoptera: Zygaenidae: Procridinae), is a pest of orchards in Japan and China. Few chemical ecological studies
have been directed towards the Zygaenidae and particularly the Procridinae. To investigate the sex pheromone of this species,
extracts of pheromone glands from adult female I. rotundata were analyzed by coupled gas chromatography-electroantennography (GC-EAG) and coupled gas chromatography-mass spectrometry
(GC-MS). Whilst GC-EAG on male moths showed an active peak, identified as 2-butyl (7Z)-dodecenoate, GC-MS also revealed the presence of the homologue 2-butyl (9Z)-tetradecenoate. Electroantennographic investigations, as well as field tests, strongly suggested the natural compounds to
have the (R)-configuration at the stereogenic centre. Field results demonstrate 0.2 mg of a 1:1-mixture of (2R)-butyl (7Z)-dodecenoate and (2R)-butyl (9Z)-tetradecenoate to be a powerful lure that may be used in pest control measures against I. rotundata. The chemical structures of the new pheromone components show the same features as those of other zygaenid species: unsaturated
fatty acids esterified with a short chain chiral alcohol. This is the first example of a two-component blend constituting
the pheromone of a procridinid species. 相似文献
3.
David Sillam-Dussès Etienne Sémon Céline Moreau Irena Valterová Jan Šobotník Alain Robert Christian Bordereau 《Chemoecology》2005,15(1):1-6
Summary. The diterpene neocembrene A or (1E,5E,9E,12R)-1,5,9-trimethyl-12-(1-methylethenyl)-1,5,9-cyclotetradecatriene, known as the trail-following pheromone of the advanced Termitidae Nasutitermitinae Nasutitermes exitiosus and Trinervitermes bettonianus, has been identified after SPME-GC/MS as the major component of the trail-following pheromone of the Rhinotermitidae Prorhinotermitinae, Prorhinotermes canalifrons and P. simplex. In all the other Rhinotermitidae studied until now, the major component of their trail pheromones is dodecatrienol ((3Z,6Z,8E)-dodeca-3,6,8-trien-1-ol). This biochemical data further add to the anatomical and molecular characteristics that give a special status to the taxon Prorhinotermes among Rhinotermitidae. In Prorhinotermes canalifrons and P. simplex, neocembrene A was the only secretory compound specific to the sternal gland surface that could be detected after SPME. It elicited orientation as well as recruitment behavioral effects. However, the comparison of the respective biological activities triggered by neocembrene A and by sternal gland secretion suggests that minor components of the latter are acting in synergy with neocembrene A. 相似文献
4.
Lilley Richard Hardie Jim Merritt L. A. Pickett John A. Wadhams Lester J. Woodcock Christine M. 《Chemoecology》1994,5(1):43-46
Summary The sex pheromone of the grain aphid,Sitobion avenae (Fab.), is identified as (4aS,7S,7aR)-nepetalactone, for which specific olfactory cells are located in the secondary rhinaria of the male antenna. The related (1R,4aS,7S,7aR)-nepetalactol was not produced by females in significant amounts (ca 10,000-fold less than the nepetalactone), although cells in the male antenna were found that responded specifically to this compound. Behavioural activity in the linear track ol-factometer showed attraction of males to the nepetalactone, but not to the nepetalactol. 相似文献
5.
Joachim Ruther 《Chemoecology》2004,14(3-4):187-192
Summary. Field tests were performed to evaluate the
response of the garden chafer, Phyllopertha horticola
(Coleoptera: Scarabaeidae) to known scarab attractants and to
investigate the influence of trap colour and height on the
number of captured beetles. Both sexes were attracted by a
lure mixture composed of geraniol, eugenol, and 2-phenylethyl
propionate (PEP) (ratio 3:7:3). When testing floral volatiles
individually, only geraniol, eugenol and methyl anthranilate
but not PEP was attractive. Response of garden chafer to (Z)-
3-hexen-1-ol was strongly male-biassed. Both the response
to floral volatiles and (Z)-3-hexen-1-ol was increased by
using funnel traps with yellow instead of grey vanes. Traps
positioned at 50 and 125 cm above ground captured significantly
more garden chafers than those at 200 cm. The
strongly male-biassed response to (Z)-3-hexen-1-ol suggests
that in P. horticola (subfamily Rutelinae) orientation towards plant volatiles emitted upon mechanical damage of plants is
part of the male mate finding strategy as recently demonstrated
for cockchafers of the genus Melolontha (subfamily Melolonthinae). Possible application of plant volatiles for
control of P. horticola is discussed. 相似文献
6.
Regine?Gries Aurélia ?Reckziegel Herman?Bogenschütz Hans-Günter?Kontzog Christian?Schlegel Wittko?Francke Jocelyn?G.?Millar Gerhard?Gries
Summary. Our objective was to identify sex pheromone
components of the oak processionary moth, Thaumetopoea
processionea (Lepidoptera: Thaumetopoeidae), whose larvae
defoliate oak, Quercus spp., forests in Eurasia and impact
human health. Coupled gas chromatographic-electroantennographic
detection (GC-EAD) and GC-mass spectrometric
(MS) analyses of pheromone gland extract of female
T. processionea revealed two consistently EAD-active compounds.
They were identified as (Z,Z)-11,13-hexadecadienyl
acetate (Z11,Z13-16:OAc) and (Z,E)-11,13,15-hexadecatrienyl
acetate (Z11,E13,15-16:OAc) by comparative GC,
GC-MS and GC-EAD analyses of insect-produced compounds
and authentic standards. In replicated field experiments
(2000, 2001) in Nordbaden, Südbaden and Sachsen-Anhalt
(Germany), Z11,Z13-16:OAc and Z11,E13,15-16:OAc in
combination, but not singly, attracted significant numbers of
male moths. It will now be intriguing to investigate whether
Z11,E13,15-16:OAc, or its corresponding alcohol or aldehyde,
serves as a pheromone component also in other species of
the Thaumetopoeidae. 相似文献
7.
X. Fu J. Tabata T. Takanashi S. Ohno S. Tatsuki Y. Ishikawa Y. Huang H. Honda 《Chemoecology》2004,14(3-4):175-180
Summary. The sex pheromone of Ostrinia orientalis
(Lepidoptera: Crambidae) was analyzed by gas chromatography–electroantennographic detection (GC–EAD), GC–mass spectrometry and a series of bioassays. Three EAD-active
compounds were detected in the female sex pheromone
gland extract, and identified as tetradecyl acetate (14:OAc),
(Z)-11-tetradecenyl acetate (Z11-14:OAc) and (E)-11-tetradecenyl acetate (E11-14:OAc). The titers (ratio) of 14:OAc, Z11-14:OAc and
E11-14:OAc in 3-day-old virgin
females were 0.49 ng (10), 4.86 ng (98) and 0.10 ng (2),
respectively. In a wind-tunnel bioassay, the 98:2 blend of
Z11- and E11-14:OAc, but not Z11-14:OAc alone, elicited
the same male behavioral responses as virgin females and
crude gland extracts. 14:OAc was inactive by itself, and did
not show any synergistic effect on the binary blend.
Field trapping experiments also confirmed the attractiveness
of the binary blend to O. orientalis
males. Based on
these results, we concluded that the sex pheromone of
O. orientalis
is a 98:2 mixture of Z11-14:OAc and
E11-14:OAc. This sex pheromone is very similar to that of the Z-type European corn borer,
O. nubilalis. The present
finding raises the question of whether O. orientalis
, which is indistinguishable from O. nubilalis
based on external morphology,
is a biologically distinct species independent from
O. nubilalis. 相似文献
8.
Summary Sex pheromone communication in the nine European species of small ermine moths (Yponomeuta) is reviewed in regard to the potential role of pheromones in the speciation process. Six of the nine species studied (viz.,Y. evonymellus, Y. cagnagellus, Y. padellus, Y. irrorellus, Y. plumbellus, andY. vigintipunctatus) use a mixture of (E)-11-and (Z)-11-tetradecenyl acetate in different ratios as primary pheromone components, with combinations of tetradecyl acetate, (Z)-9-tetradecenyl acetate, (Z)-11-hexadecenyl acetate and the corresponding alcohols of the acetates as additional pheromone components. Analysis of (Z)- to (E)-11-tetradecenyl acetate ratios produced by individual females of these species demonstrated significant variation among females of all species. However, the ranges of ratios produced byY. cagnagellus, Y. irrorellus, andY. plumbellus, sharing the same host-plant species, spindle tree, did not overlap. Niche separation of all six species mentioned required consideration of at least one additional pheromone component or of temporal aspects. The remaining three species,i.e. Y. malinellus, Y. mahalebellus andY. rorellus, have pheromones that differ qualitatively.Biosynthetic routes to the pheromone components identified are proposed on the basis of fatty acid pheromone precursors found in the pheromone glands. A phylogenetic tree for the genus is constructed based on allozyme frequency data and changes in pheromone composition are superimposed on this tree. We suggest that the ancestral ermine moth pheromone is a mixture of (Z)-11- and (E)-11-tetradecenyl acetate and the corresponding alcohols, and a scenario of how present-day patterns evolved is outlined. The pheromone differences among the three species using spindle tree as their host-plant might have evolved throughreproductive character displacement upon secondary contact between populations that had already diverged genetically in allopatry. Pheromone differences within the so-calledpadellus-complex (includingY. cagnagellus, Y. mahalebellus, Y. malinellus, Y. padellus, andY. rorellus) in which species might have originated sympatrically, may have evolved byreinforcing selection as these species still hybridise and produce viable offspring when confined in cages. The role of pheromones in reproductive isolation amongYponomeuta species is emphasised by (1) the function of pheromone components of some of the species as behavioural antagonists to other species, (2) the cross-attraction under experimental conditions between allochronic species with similar pheromones, and (3) the formation of hybrids in the laboratory between species that are isolated in nature by pheromone differences. 相似文献
9.
Jim Hardie Stephen F. Nottingham Glenn W. Dawson Richard Harrington John A. Pickett Lester J. Wadhams 《Chemoecology》1992,3(3-4):113-117
Summary Transparent plastic water traps, baited with the synthetic aphid sex pheromone components (4aS,7S,7aR)-nepetalactone and (1R,4aS,7S,7aR)-nepetalactol or control lures, were placed in four semi-sheltered field sites at a height of 1.1 m. Although males of twenty-one aphid species were collected from the water traps, onlySitobion fragariae (Walker) was abundant. In total, 8 pheromone traps produced 102 males compared with only 10 males in 8 control traps. The sex pheromone released by sexual femaleS. fragariae was identified as the nepetalactone used in the lures. Aphid sex pheromones may be more species-specific than previously thought and the presence of a host plant is not essential for males to locate sexual females. 相似文献
10.
Summary. Females of both species start their pheromone-releasing activity on the second day after emergence at the beginning of the
photophase. During the present work, a peak of calling activity with close to 100% of active Ph. nigrescentella females was registered 1.5 hour after the light had been put on. The high pheromone release behaviour with 50% active females
lasted for 3 hours. The calling activity of the group of females was about 6 h/day. The beginning of a photophase under laboratory
conditions or an early morning in nature is a common period for sex pheromone release in the genus Phyllonorycter. (8Z,10E)-tetradecadien-1-yl acetate (8Z,10E-14:Ac), (8Z,10E)-tetradecadien-1-ol (8Z,10E-14:OH) and (8E,10Z)-tetradecadien-1-yl acetate (8E,10Z-14:Ac) in the ratio 96:4:traces as well as 8Z,10E-14:Ac and 8Z,10E-14:OH in the ratio 88:12 collected by Solid Phase Micro Extraction (SPME) were found to be specific for the calling periods
of virgin Phyllonorycter insignitella and Ph. nigrescentella females respectively. Field trapping experiments demonstrated that all three compounds are important for the attraction of
Ph. insignitella males while only 8Z,10E-14:Ac is the essential sex pheromone component for Ph. nigrescentella. The pheromone activity of all three compounds is reported for the first time. Addition of either 8Z,10E-14:OH or 8E,10Z-14:Ac to 8Z,10E-14:Ac did not have a significant effect on the attraction of Ph. nigrescentella males, while the efficiency of the three component blend was 5 times lower as compared to that of 8Z,10E-14:Ac. Our data demonstrate that 8Z,10E-14:OH and 8E,10Z-14:Ac play a dual function, they are minor sex pheromone components of Ph. insignitella essential for attraction of conspecific males and show an allelochemical, antagonistic effect on Ph. nigrescentella males and, thus, ensuring specificity of the mate location signal in two related Phyllonorycter species. 相似文献
11.
Martin J. Steinbauer Fredrik Östrand Tom E. Bellas Anna Nilson Fredrik Andersson Erik Hedenström Michael J. Lacey P. Florian Schiestl 《Chemoecology》2004,14(3-4):217-223
Summary. The autumn gum moth, Mnesampela privata
(Guenée) (Lepidoptera: Geometridae), is native to Australia
and can be a pest of plantation eucalypts. Field-collected
and laboratory-reared female autumn gum moths were
dissected to remove glands likely to contain components of
the sex pheromone. Using gas chromatography (GC) and
combined gas chromatography–mass spectrometry (GC-MS),
three compounds were identified from female extracts,
namely (3Z,6
Z,9
Z)-3,6,9-nonadecatriene, 1-hexadecanol
and 1-octadecanol (confirmed by comparison with synthetic
samples). Nonadecatriene elicited an antennal response in
male autumn gum moth during gas chromatographic
analyses combined with electroantennographic detection
(GC-EAD). In electroantennogram (EAG) recording male
M. privata antennae responded to the nonadecatriene. Nonadecatriene was synthesised via Kolbe electrolysis,
starting with (9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid
(linolenic acid) and propanoic acid or via an alternative
four-step method also starting from linolenic acid. In field
trials (3Z,6Z,9Z)-3,6,9-nonadecatriene proved attractive
to male moths. Thus, we conclude that (3Z,6Z,9Z)-3,6,9-
nonadecatriene is a sex pheromone component of autumn
gum moth. This component has been identified in extracts
from other geometrids in the same subfamily, Ennominae.
However, to our knowledge this is the first example where
(3Z,6Z,9Z)-3,6,9-nonadecatriene has been found in females
and also proved attractive to male moths when presented on its
own. Our results are discussed in relation to other geometrid
pheromones. 相似文献
12.
Summary. In a local population of Ostrinia zaguliaevi Mutuura & Munroe (Lepidoptera: Crambidae), extensive variation was found in the blend ratio of three sex pheromone components,
(Z)-9-tetradecenyl acetate (Z9-14:OAc, 10.2-63.8%), (Z)-11-tetradecenyl acetate (Z11-14:OAc, 32.2-86.8%), and (E)-11-tetradecenyl acetate (E11-14:OAc, 2.1-11.9%). The variation was observed over a three-year period (2002-2004). Mother-daughter
regression analyses have shown that although the heritability of the minor component E11-14:OAc was not significant, the heritability
of the proportions of Z9-14:OAc and Z11-14:OAc were substantial (0.5–0.6). In artificial selection experiments, the mean % Z9-14:OAc in the sex pheromone changed significantly within three generations (37% in the control line, 48 and 52% for two
lines selected for increase, and 23 and 30% for two lines selected for decrease). Despite these changes, the amounts of fatty
acyl pheromone precursors, (Z)-9-, (Z)-11- and (E)-11-tetradecenoate, in the pheromone gland were not significantly affected by the selection. Taken together, variation in
the pheromone blend of O. zaguliaevi is likely to be attributable to a few genes involved in the reduction or acetylation of fatty acyl pheromone precursors,
the last two steps in pheromone biosynthesis. 相似文献
13.
Robert R. Carlton Stanley G. Deans Alexander I. Gray Peter G. Waterman 《Chemoecology》1991,2(1):69-71
Summary The antifungal activity of kaempferol-3-(2,3-diacetoxy-4-p-coumaroyl)rhamnoside, a new flavonol glycoside isolated from the leaves ofMyrica gale, has been investigated. The flavonoid was found to have varying inhibitory activity against five species of fungi isolated from the leaves ofMyrica gale in the field. 相似文献
14.
Summary. Three components that elicited antennal response from male Choristoneura conflictana were found from female gland extracts analyzed using a coupled gas chromatographic-electroantennographic detector system.
The main component in gland extracts was (Z)-11-tetradecenal (Z11-14:Ald). Two minor components also elicited antennal response: (E)11-tetradecenal (E11-14:Ald) and (Z)-11- tetradecen-1-ol (Z11-14:OH). Analysis of effluvia indicated that calling virgin females release mostly Z11-14:Ald and trace amounts of Z11-14:OH. Field and wind tunnel behavioral studies showed that Z11-14:Ald alone attracted male moths in a dose response pattern. Tests comparing male response to blends of components detected
in gland extracts showed that addition of 1.8% of E11-14:Ald to Z11-14:Ald did not influence male moths in the wind tunnel, but resulted in significantly lower trap captures in the field.
The threecomponent blend [Z11-14:Ald (100), E11-14:Ald (1.8), Z11-14:OH (11)], was less attractive than Z11-14:Ald alone in both field and wind tunnel studies. Traps baited with two virgin female moths were equally attractive to
males as traps baited with the three-component synthetic blend but less attractive than traps baited with Z11-14:Ald alone. Field tests of various blends of the two components (Z11-14:Ald, Z11-14:OH) detected in the females’ effluvia showed that the addition of 1–10% Z11-14:OH to Z11-14:Ald did not affect the males’ response to Z11-14:Ald. Our data demonstrate that female C. conflictana release sex pheromone components in a different ratio than they are stored in the pheromone gland. The sex pheromone is comprised
of a single component, Z11-14:Ald, that can be used to monitor mated and virgin male C. conflictana throughout their flight period. 相似文献
15.
Summary. The major component of the trail pheromone of
the myrmicine ant Crematogaster castanea
has been identified as (R)-2-dodecanol from
the tibial glands of the hind legs. The substance gave activity comparable to the
contents of 8 tibial glands at a concentration of 1 pg per 32 cm trail. 相似文献
16.
Summary. The basic chemical structure of the sex
pheromone of the pine sawfly Microdiprion pallipes
(Fallén) has earlier been identified as the propionate
ester of (2S,3S,7R/S,11R/S)/(2R,3R,7R/S,11R/S)-3,7,11-
trimethyl-2-tridecanol. We now report the results from
further investigations on the male response to individual
stereoisomers and to blends of stereoisomers, both in electroantennographic
(EAG) recordings and in field trapping
experiments. We also present our attempts to determine the
stereochemistry of the compounds present in females of
M. pallipes. By comparing gas chromatograms and mass
spectra obtained from natural extracts with those from synthetic
compounds it was found that the females contain one
or more of the four (2S,3S,7R/S,11R/S)-3,7,11-trimethyl-2-
tridecanol isomers (SS++-1). The active pheromone component
is the corresponding propionate ester 2. In EAG
experiments, males responded most strongly to five propionate
ester samples, namely two four-isomer blends: SS++-2
and SR++-2, and three individual stereoisomers: SSSR-,
SRRR- and SRSR-2. In a series of field trapping experiments
it was found that males were attracted to the SR++-2
four-isomer blend and to the individual isomer SSSR-2.
Based on the EAG-recordings and field responses of males
and the stereoisomers found in the females, we suggest that
the propionate ester of (2S,3S,7S,11R)-3,7,11-trimethyl-2-
tridecanol (SSSR-2) is used as a main component of the sex
pheromone in M. pallipes. Apparently the males react to
other stereoisomers in addition to that or those produced by
the females. 相似文献
17.
The bark beetles Pityogenes bidentatus and Pityogenes quadridens (Coleoptera, Curculionidae, Scolytinae) are sibling species that feed and reproduce in bark areas on branches of Scotch pine, Pinus sylvestris. To identify aggregation pheromone components of both species, hindguts and head/thoraxes of males and females of both species feeding in hosts were extracted in pentane and analyzed by gas chromatography and mass spectrometry. Hindguts of male P. bidentatus contained grandisol as the major component along with small amounts of (4S)-cis-verbenol and other monoterpenes. Dose–response bioassays in the laboratory showed the components were attractive at 0.2 ng/min to walking beetles from a distance of ≥25 cm. In the field in southern Sweden, grandisol and (4S)-cis-verbenol were weakly attractive alone when released at rates of 0.05 and 0.5 mg/day, respectively, from a slow-rotating trap pair. Catch increased 3.6- to 13-fold when the two components were released together. The male proportion of the catch was 0.8 early in the flight period, declined to 0.5 on the peak flight day, and then declined further during the next 2 weeks to 0.2 on the last day of the flight period. Hindguts of male P. quadridens contained (2S,5R)- and (2S,5S)-chalcogran, as well as (E)-2-(3,3-dimethylcyclohexylidene)ethanol (E-grandlure 2) and E/Z-mixture of 2-(3,3-dimethylcyclohexylidene)acetaldehyde (grandlures 3 and 4), while female hindguts had only a trace amount of chalcogran. Laboratory studies proved E-grandlure 2 is an essential pheromone component for P. quadridens. Field bioassays with a slow-rotating trap pair in which the attractiveness of blends containing various candidate components were compared with that of less complete mixtures, showed that chalcogran and E-grandlure 2 were synergistic aggregation pheromone components of P. quadridens. Field tests also showed that grandisol (from P. bidentatus) reduced attraction of P. quadridens to its aggregation pheromone, whereas E-grandlure 2 and chalcogran (from P. quadridens) reduced response of P. bidentatus to its aggregation pheromone. Our results suggest that aggregation pheromone components from males of each species not only attract conspecifics but also aid individuals in avoiding interspecific mating and competition for food and spatial resources within the bark phloem layer. 相似文献
18.
Summary
Ips duplicatus withI. typographus co-inhabiting Norway spruce (Picea abies (L.) Karst.) would benefit from a pheromone blend distinct from that of the larger competitorI. typographus. GC-MS analysis showed thatI. duplicatus males feeding in the host produced ipsdienol (Id),cis-verbenol (cV),trans-verbenol (tV), myrtenol (Mt), andE-myrcenol (EM) and traces of 2-methyl-3-buten-2-ol (MB).I. duplicatus produced Id in approximately racemic form (48.9-54.5% (+)-(S)-isomer). The amounts of Id and EM released over a 9 day period had a maximum of 250 and 5 ng/h/male, respectively, on day 2. Exposure ofI. duplicatus males to myrcene and -pinene resulted in the production of small amounts of Id, cV, tV, Mt, andtrans-pinocarveol, but not of EM. In laboratory bioassays with walking beetles, the pheromone component Id alone was weakly attractive while EM was inactive, but in binary combination with Id strongly synergized attraction. A combination of EM and Id at a release rate equivalent to 100–200 males was more attractive in the field than 70 unmated males in a spruce log. The addition of myrcene ( a suggested pheromone precursor of Id) to Id did not enhance trap caches, while addition of EM increased catches > 10-fold. Subtracting EM from a blend of Id, EM, cV and MB drastically reduced trap catches while subtraction of cV or MB or both had no significant effect. Addition of EM over a wide concentration range to the synthetic pheromone ofI. typographus did not reduce the attraction of females of this species in the laboratory. A two-species pheromone interaction field test releasingI. typographus pheromone components (MB + cV) at 10–1000 male equivalents (ME) andI. duplicatus pheromone (Id + EM) at 0, 10–1000 ME in all possible combinations showed both positive intraspecific dose-response effects and an interspecific inhibition. Higher release rates of EM appeared to inhibitI. typographus, especially males. In a tree colonization model, the response of the two competing species to their respective pheromones show a good separation during the mass-attack with a small initial cross-attraction. It remains to be shown whether either of the two pheromone systems have in fact evolved in the present sympatry, or if they are an incidental effect of ancestry of these phylogenetically distantIps. 相似文献
19.
Summary. Male locust borers, Megacyllene robiniae
(Förster), responded to females only after contacting them
with their antennae, indicating that mate recognition was
mediated by a contact sex pheromone. GC-MS analyses of
whole-body extracts of males and females determined that the
profiles of compounds in the extracts were qualitatively similar,
but differed considerably in the ratios of compounds
between sexes. Biological activities of reconstructed blends
of the most abundant straight-chain (nC23, nC24, nC25, nC26),
methyl-branched (3me-C23, 3me-C25), and unsaturated
(Z9:C23, Z9:C25, Z9:C27 compounds in extracts from females
were assessed in arena bioassays, assessing four distinct steps
in the mating behavior sequence of males (orientation, arrestment,
body alignment, mounting and attempting to couple the
genitalia). Males were unresponsive to freeze-killed, solventwashed
females treated with blends of straight-chain and
methyl-branched alkanes, but responded strongly to females
treated with the blend of alkenes. Further trials determined
that the complete sequence of mating behaviors, up to and
including coupling the genitalia, was elicited by Z9:C25 alone.
Z9:C25 comprised 16.4 ± 1.3% of the total hydrocarbons in
whole-body hexane extracts of females and was co-dominant
with two other hydrocarbons that were not active. In contrast,
in solid phase microextraction (SPME) wipe samples from
several areas of the cuticle, Z9:C25 appeared as the single
dominant peak, comprising 34.6 – 37.8% of the sampled
hydrocarbons. Our data indicate that Z9:C25 is a contact sex
pheromone of M. robiniae, being the most abundant hydrocarbon
on the surface of the cuticular wax layer of females
where it is readily accessible to the antennae of males. 相似文献
20.
大同盆地是典型的高砷地下水分布区。利用从地方性砷中毒严重病区山阴县采集的高砷地下水样品,用稀释培养法实验研究了外加砷源对地下水中微生物数量的影响;同时基于生物学可培养法和16S rDNA序列比对法,选取代表性高砷水样,研究了耐砷菌的种群特征。结果表明,外加砷源对地下水中微生物数量影响显著,高浓度砷会抑制大部分微生物生长,使微生物数量减少;低浓度砷对微生物生长具有一定促进作用。通过多次分离、纯化从3个不同砷含量地下水样中分离到多株砷抗性菌,经鉴定属于主要为Bacillus、Pseudomonas、Paenibacillus、Aeromonas、Enterobacter5个属。从RDP(Ribosomal Database Project)分析显示3个水样可培养微生物组成不同,都有生存能力强能够耐低浓度NaAsO2的Bacillales,优势耐砷菌是γ-proteobacteria,其中Enterbacter具有耐高浓度NaAsO2的能力。 相似文献