短程硝化反硝化生物脱氮的实现途径

短程硝化反硝化是近年来开发的一项新的生物脱氮技术,它具有节约曝气量、节省有机碳源和减少污泥产量等优点,实现短程硝化反硝化生物脱氮技术的关键就是将硝化控制在亚硝酸阶段,阻止亚硝酸盐的进一步氧化。简要介绍了短程硝化反硝化生物脱氮技术的原理和技术特点,通过对短程硝化反硝化影响因素的分析来介绍其实现途径,阐述了温度控制途径、溶解氧控制途径、pH值控制途径、投加抑制剂途径、泥龄控制途径以及纯种分离与固定化技术途径的控制机理及其局限性,并提出了今后的研究方向。     

短程硝化--反硝化生物脱氮工艺的研究进展

短程硝化反硝化生物脱氮工艺是将硝化控制在形成亚硝酸阶段,阻止亚硝酸的进一步硝化,然后直接进行反硝化。本文结合国内外的研究,对短程硝化脱氮技术的实现途径进行了概括和探讨,对该工艺的开发应用（如SHARON工艺、OLAND工艺、CANONT艺和生物膜／活性污泥法结合的短程硝化反硝化工艺）作了简要综述,并指出了该工艺的技术优势和应用价值。     

短程硝化-反硝化生物脱氮技术研究

将短程硝化-反硝化生物脱氮技术与传统生物脱氮技术进行了比较,论述了短程硝化-反硝化生物脱氮技术的机理及特点。分析了实现亚硝酸盐积累的影响因素,包括温度、溶解氧浓度、pH值、分子态游离NH3浓度和泥龄。结合典型工艺,提出了目前短程硝化-反硝化脱氮技术存在的问题及改进建议。     

污水处理厂短程硝化影响因素分析及效果评估

文章基于采用厌氧-缺氧-好氧法(A²O)工艺在某城市污水处理厂形成的稳定短程硝化现象，分析了不同影响因素与短程硝化形成的关联性，评估了实现短程硝化对于该厂能耗、药耗及污染物去除效果的实际影响。     

间歇曝气短程硝化控制新途径的初步试验研究

采用SBR,以间歇曝气的方式控制短程硝化,分别研究了在限制曝气量和不限制曝气量条件下,亚硝酸盐的积累情况.试验表明,间歇曝气可以作为短程硝化的控制条件,在一定条件下,亚硝化率可达到90%以上.限制曝气量时,在低DO情况下,采用间歇曝气控制短程硝化更为有效、经济.     

SBR法处理垃圾渗滤液及其同时硝化反硝化生物脱氮研究

采用SBR系统处理城市垃圾渗滤液,研究了不同C／N、130和MLSS对同时硝化反硝化脱氮效率的影响。结果表明：总氮去除率随着C／N、MLSS升高而上升;DO越低,总氮去除率越高;当进水CODCr与NH3-N浓度分别为420mg／L和112mg／L,DO和MLSS分别为1．5mg／L和5016mg／L时,CODCr、NH3-N及TN去除率分别为81．54％、96．57％和46．66％。根据试验结果,对同时硝化反硝化一个代表周期作了分析。     

同步硝化好氧反硝化生物脱氮机理分析及其研究进展

通过对比传统生物脱氮理论，提出同步硝化好氧反硝化技术优点，对好氧反硝化的机理进行了初步探讨，并从不同角度做了理论分析。同时阐述了同步硝化反硝化技术的控制因素及其研究进展。并对好氧反硝化的应用前景作了展望，提出了好氧反硝化今后的研究方向。     

多孔颗粒载体-膜生物反应器脱氮效果研究

实验探索了高MLSS(溶液悬浮固体浓度)下多孔颗粒载体—膜生物反应器发生短程硝化、反硝化的可能性。研究了溶解氧(DO)、C/N、氨浓度及氨负荷、pH等因素对短程硝化、反硝化和TN去除的影响。实验发现,当控制pH=7.5、DO=1.0mg/L、C/N为6时,该反应器能同时达到较高的NH4 —N和TN出除率。     

SBR系统中pH与MLSS对同步硝化反硝化的影响

本文研究了SBR系统中pH值、MISS的变化对同步硝化反硝化的影响。结果表明：在进水水质和反应条件相同时,将pH值控制在8．5,出水水质最好,COD去除率达到90．0％,总氮去除率达到99．4％;在进水水质和反应条件相同,反应器中MLSS为520mg／L时,出水水质最好,COD去除率达到85馏％,总氮去除率达到99．1％。     

同时硝化反硝化生物脱氮技术研究进展

对同时硝化反硝化（SND）生物脱氮新技术的机理进行了较全面的探讨，对影响SND的控制因素及其研究现状进行了简单的综述，最后指出了实现SND存在的一些急需解决的问题。     

Rapid Assessment of Urban Wetlands: Do Hydrogeomorphic Classification and Reference Criteria Work?

The Hydrogeomorphic (HGM) functional assessment method is predicated on the ability of hydrogeomorphic wetland classification and visual assessment of alteration to provide reference standards against which functions in individual wetlands can be evaluated. The effectiveness of this approach was tested by measuring nitrogen cycling functions in forested wetlands in an urbanized region in New Jersey, USA. Fourteen sites represented three HGM classes and were characterized as “least disturbed reference” or “non-reference” based on initial visual assessment. Water table levels and in situ rates of net nitrogen mineralization, net nitrification, and denitrification were measured over one year in each site. Hydrological alterations, resulting in consistently low or flashy water table levels, were not correlated with a priori designations as reference and non-reference. Although the flat-riverine wetland class had lower net nitrification and higher denitrification rates than riverine or mineral flat wetland classes, this difference was attributable to the lack of hydrologically-altered wetlands in the flat-riverine class, and thus more consistently wet conditions. Within all HGM classes, a classification based on the long-term hydrological record that separated sites with “normal,” saturated hydrology from those with “altered,” drier hydrology, clearly distinguished sites with different nitrogen cycling function. Based on these findings, current practices for designating reference standard sites to judge wetland functions, at least in urbanized regions, are ineffective and potentially misleading. At least one year of hydrological monitoring data is suggested to classify wetlands into groups that have different nutrient cycling functions, particularly in urban landscapes.     

Potential nitrification and denitrification on different surfaces in a constructed treatment wetland

Improved understanding of the importance of different surfaces in supporting attached nitrifying and denitrifying bacteria is essential if we are to optimize the N removal capacity of treatment wetlands. The aim of this study was therefore to examine the nitrifying and denitrifying capacity of different surfaces in a constructed treatment wetland and to assess the relative importance of these surfaces for overall N removal in the wetland. Intact sediment cores, old pine and spruce twigs, shoots of Eurasian watermilfoil (Myriophyllum spicatum L.), and filamentous macro-algae were collected in July and November 1999 in two basins of the wetland system. One of the basins had been constructed on land that contained lots of wood debris, particularly twigs of coniferous trees. Potential nitrification was measured using the isotope-dilution technique, and potential denitrification was determined using the acetylene-inhibition technique in laboratory microcosm incubations. Nitrification rates were highest on the twigs. These rates were three and 100 times higher than in the sediment and on Eurasian watermilfoil, respectively. Potential denitrification rates were highest in the sediment. These rates were three times higher than on the twigs and 40 times higher than on Eurasian watermilfoil. The distribution of denitrifying bacteria was most likely due to the availability of organic material, with higher denitrification rates in the sediment than on surfaces in the water column. Our results indicate that denitrification, and particularly nitrification, in treatment wetlands could be significantly increased by addition of surfaces such as twigs.     

Seasonal dynamics of denitrification along topohydrosequences in three different riparian wetlands

We investigated the seasonal patterns of denitrification rates and potentials in soil profiles along the topohydrosequence formed at the upland-wetland interface in three riparian wetlands with different vegetation cover (i.e., forest, understory vegetation, and grass). Denitrification was measured using the acetylene inhibition method on soil cores and slurries, which provided a means of comparing the relative activity of this process in different locations. We evaluated, on a seasonal basis, the respective importance of the vegetative cover and the hydromorphic gradient as factors limiting denitrification. Regardless of the season, vegetation type, or lateral position along each topohydrosequence in the riparian wetlands, strong significant gradients of both in situ and potential denitrification rates were measured within a soil profile. Results confirm that the upper organic soil horizon is the most active, when in contact with the ground water. In deeper soil horizons, denitrification activity was low (from 0.004 to 0.5 mg N kg(-1) dry soil d(-1)), but contributed significantly to the reduction of ground water NO3- load along the riparian ground water flowpath (from 9.32 to 0.98 mg NO3-N L(-1)). Along the soil topohydrosequence, the denitrifying community of the upper soil horizons did not vary significantly on a seasonal basis despite the large seasonal ground water fluctuations. Along each topohydrosequence, the denitrification-limiting factor gradually shifted from anaerobiosis to NO3- supply. In situ denitrification rates in the forested, understory vegetation and grass sites were not significantly different. This result emphasizes the importance of the topography of the valley rather than the vegetation cover in controlling denitrification activity in riparian wetlands.     

Quantifying nitrogen process rates in a constructed wetland using natural abundance stable isotope signatures and stable isotope amendment experiments

This study describes the spatial variability in nitrogen (N) transformation within a constructed wetland (CW) treating domestic effluent. Nitrogen cycling within the CW was driven by settlement and mineralization of particulate organic nitrogen and uptake of NO3-. The concentration of NO3- was found to decrease, as the delta15N-NO3- signature increased, as water flowed through the CW, allowing denitrification rates to be estimated on the basis of the degree of fractionation of delta15N-NO3-. Estimates of denitrification hinged on the determination of a net isotope effect (eta), which was influenced byprocesses that enrich or deplete 15NO3- (e.g., nitrification), as well as the rate constants associated with the different processes involved in denitrification (i.e., diffusion and enzyme activity). The influence of nitrification on eta was quantified; however, it remained unclear how eta varied due to variability in denitrification rate constants. A series of stable isotope amendment experiments was used to further constrain the value of eta and calculate rates of denitrification, and nitrification, within the wetland. The maximum calculated rate of denitrification was 956 +/- 187 micromol N m(-2) h(-1), and the maximum rate of nitrification was 182 +/- 28.9 micromol N m(-2) h(-1). Uptake of NO3- was quantitatively more important than denitrification throughoutthe wetland. Rates of N cycling varied spatially within thewetland, with denitrification dominating in the downstream deoxygenated region of the wetland. Studies that use fractionation of N to derive rate estimates must exercise caution when interpreting the net isotope effect. We suggest a sampling procedure for future natural abundance studies that may help improve the accuracy of N cycling rate estimates.     

Denitrification in alluvial wetlands in an urban landscape

Riparian wetlands have been shown to be effective "sinks" for nitrate N (NO3-), minimizing the downstream export of N to streams and coastal water bodies. However, the vast majority of riparian denitrification research has been in agricultural and forested watersheds, with relatively little work on riparian wetland function in urban watersheds. We investigated the variation and magnitude of denitrification in three constructed and two relict oxbow urban wetlands, and in two forested reference wetlands in the Baltimore metropolitan area. Denitrification rates in wetland sediments were measured with a 15N-enriched NO3- "push-pull" groundwater tracer method during the summer and winter of 2008. Mean denitrification rates did not differ among the wetland types and ranged from 147 +/- 29 microg N kg soil(-1) d(-1) in constructed stormwater wetlands to 100 +/- 11 microg N kg soil(-1) d(-1) in relict oxbows to 106 +/- 32 microg N kg soil(-1) d(-1) in forested reference wetlands. High denitrification rates were observed in both summer and winter, suggesting that these wetlands are sinks for NO3- year round. Comparison of denitrification rates with NO3- standing stocks in the wetland water column and stream NO3- loads indicated that mass removal of NO3- in urban wetland sediments by denitrification could be substantial. Our results suggest that urban wetlands have the potential to reduce NO3- in urban landscapes and should be considered as a means to manage N in urban watersheds.     

Evaluating stream water quality through land use analysis in two grassland catchments: impact of wetlands on stream nitrogen concentration

We evaluated the impacts of natural wetlands and various land uses on stream nitrogen concentration in two grassland-dominated catchments in eastern Hokkaido, Japan. Analyzing land use types in drainage basins, measuring denitrification potential of its soil, and water sampling in all seasons of 2003 were performed. Results showed a highly significant positive correlation between the concentration of stream NO3-N and the proportion of upland area in drainage basins in both catchments. The regression slope, which we assumed to reflect the impact on water quality, was 24% lower for the Akkeshi catchment (0.012 +/- 0.001) than for the Shibetsu catchment (0.016 +/- 0.001). In the Akkeshi catchment, there was a significant negative correlation between the proportion of wetlands in the drainage basins and stream NO3-N concentration. Stream dissolved organic nitrogen (DON) and carbon (DOC) concentrations were significantly higher in the Akkeshi catchment. Upland and urban land uses were strongly linked to increases in in-stream N concentrations in both catchments, whereas wetlands and forests tended to mitigate water quality degradation. The denitrification potential of the soils was highest in wetlands, medium in riparian forests, and lowest in grasslands; and was significant in wetlands and riparian forests in the Akkeshi catchment. The solubility of soil organic carbon (SOC) and soil moisture tended to determine the denitrification potential. These results indicate that the water environment within the catchments, which influences denitrification potential and soil organic matter content, could have caused the difference in stream water quality between the two catchments.     

Denitrification in constructed wetlands used for treatment of swine wastewater

Constructed wetland treatment of swine wastewater probably involves substantial denitrification. Our objective was to assess denitrification and denitrification enzyme activity (DEA) in such wetlands in relation to plant communities, N loading, carbon or nitrogen limitations, and water depth. Two wetland cells each 3.6 m wide and 33.5 m long were connected in series. One set of cells was planted with rushes and bulrushes, including soft rush (Juncus effusus L.), softstem bulrush [Schoenoplectus tabernaemontani (K.C. Gmel.) Pallal, American bulrush [Schoenoplectus americanus (Pers.) Volkart ex Schinz & R. Keller], and woolgrass bulrush [Scirpus cyperinus (L.) Kunth]. Another set was planted with bur-reeds and cattails, including American bur-reed (Sparganium americanum Nutt.), broadleaf cattail (Typha latifolia L.), and narrowleaf cattail (Typha angustifolia L.). The sets will be referred to herein as bulrush and cattail wetlands, respectively. Denitrification and DEA were measured via the acetylene inhibition method in intact soil cores and disturbed soil samples that were taken during four years (1994-1997). Although DEA in the disturbed samples was greater than denitrification in the core samples, the measurements were highly correlated (r2 > or = 0.82). The DEA was greater in the bulrush wetlands than the cattail wetlands, 0.516 and 0.210 mg N kg(-1) soil h(-1), respectively; and it increased with the cumulative applied N. The DEA mean was equivalent to 9.55 kg N ha(-1) d(-1) in the bulrush wetlands. We hypothesized and confirmed that DEA was generally limited by nitrate rather than carbon. Moreover, we determined that one of the most influential factors in DEA was wetland water depth. In bulrush wetlands, the slope and r2 values of the control treatment were -0.013 mg N kg(-1) soil h(-1) mm(-1) depth and r2 = 0.89, respectively. Results of this investigation indicate that DEA can be very significant in constructed wetlands used to treat swine wastewater.