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1.
深圳湾浮游植物的季节变化 总被引:1,自引:0,他引:1
2008年2月至11月对深圳湾的浮游植物和理化环境因子进行了4个季度月的调查,结果共检出浮游植物150种(包括变种和变型):春季66种、夏季72种、秋季54种、冬季50种,其中硅藻门36属108种,甲藻门14属36种,绿藻门3属3种,蓝藻门2属3种。优势种共有湖沼圆筛藻Coscinodiscus lacustris、中肋骨条藻Skeletonema costatum、夜光藻Noctiluca scientillans 3种:春季1种、夏季1种、秋季1种、冬季2种,优势种群由春夏季的湖沼圆筛藻演替至秋季的中肋骨条藻、冬季的中肋骨条藻和夜光藻,没有全年广布优势种;4季均出现的种类共有9种,其中硅藻8种,甲藻1种,各季节间共有种类数在13~31种,Jaccard种类相似性指数范围在0.12~0.35,季节更替明显。多样性指数和均匀度的变化范围分别为0.006~1.724和0.001~0.306,群落结构较脆弱。细胞密度在1.25×107~217.90×107 cells.m-3,夏季最高,春季次之,冬季最低,属季节单峰型变化,与一般亚热带春、秋季出现密度高峰不一致,这与深圳湾陆源营养物质的扰动有关,其无机氮和活性磷酸盐含量均劣于国家海水水质标准的四类水,因此,该海域水质营养类型属于亚热带富营养型。细胞密度与硅酸盐呈极显著的负相关,相关系数为-0.446(p〈0.01,n=36,双尾),与水温呈显著的正相关,相关系数为0.371(p〈0.05,n=36,双尾),与其他因子的相关性不明显。从优势种的种类数和多样性指数分析,深圳湾浮游植物的群落结构已趋于单一化,生态系统抗干扰能力极为脆弱。 相似文献
2.
From 18 to 23 September 1974, investigations on the diel changes in phytoplankton were carried out in the Baltic Sea. Every 4 h, water samples were collected from 2 and 15 m, and PO4, chlorophyll a, temperature, salinity, pH, phytoplankton composition and phytoplankton light photosynthesis relationship were determined. Continuous measurements of surface irradiance and some estimations of zooplankton were also made. P
B
(photosynthesis per unit chlorophyll a at low light levels of 2·10-2 cal cm-2 min-1) revealed only random variation during the sampling period, i.e., 1.0 to 1.6 mg C (mg chlorophyll a)-1 h-1. P
m
B
(Light-saturated photosynthesis per unit of chlorophyll a) displayed pronounced diel fluctuations with the highest value of about 6 mg C (mg chlorophyll a)-1 h-1 around noon, and the lowest value of about 2.5 mg C (mg chlorophyll a)-1 h-1 during the night, during which latter period the value of P
m
B
was more or less constant. Reasons for the diel fluctuations are discussed, and an equation which describes these fluctuations is proposed. Using this equation, the daily phytoplankton production estimated in incubators by a previously described method can be corrected for the time of day at which samples are collected. 相似文献
3.
Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: Evaluation and field results 总被引:67,自引:0,他引:67
To assess bacterioplankton production in the sea, we have developed a procedure for measuring growth based on incorporation of tritiated thymidine into DNA; the accuracy of this procedure was tested under a variety of laboratory and field conditions. By autoradiography, we have found that for all practical purposes our technique is specific for the nonphotosynthetic bacteria and that virtually all of the active bacteria (one-third or more of the total countable bacteria) take up thymidine. We also measured (1) the intracellular isotope dilution of thymidine assessed by parallel experiments with labeled phosphorus, and (2) DNA content of natural marine bacteria (0.2 to 0.6 m size fraction); a conversion factor derived from these data permitted estimation of production from thymidine incorporation results. A very similar conversion factor was independently derived from the empirical relationship between thymidine incorporation and growth of natural bacterioplankton under controlled conditions. Combined results show that this technique, which can be performed rapidly and easily at sea, provides good estimates of production. Data from Southern California Bight waters, which contain oligotrophic as well as moderately eutrophic regions, show that average bacterioplankton doubling times, like those of the phytoplankton, are on the order of a few days, with fastest growth at depths just below those of greatest phytoplankton abundance. Offshore bacterial production is roughly 5 to 25% of the primary production; thus, at a 50% assimilation efficiency, the bacterioplankton would consume 10 to 50% of the total fixed carbon. 相似文献
4.
Measurement of a respiratory control ratio is deemed very useful in determination of energy balance in respiration. We have defined and computed a control ratio as the ratio of the rate of oxygen consumption in vivo to the calculated maximum rate of oxygen consumption in vitro as measured by enzymatic electron transport rates. In order for a control ratio to serve a useful purpose in calculating in situ oxygen consumption in a water parcel, the ratio must be fairly constant. Our results indicated that this is precisely so in healthy cultures of the 9 species of phytoplankton studied. The data presented in this communication further point towards the potential for making reasonable estimates of phytoplankton oxygen consumption in an ocean water parcel by directly utilizing in vitro electron transport activity measurements. 相似文献
5.
Planktonic bacteria in the Humber Estuary; Seasonal variation in population density and heterotrophic activity 总被引:1,自引:0,他引:1
Seasonal variation in population density and heterotrophic activity (as maximum potential rate, V
max, for glucose mineralization) of bacteria attached to suspended solids, of free bacteria and of total bacteria was examined in the water of the Humber Estuary, north-east England. At the principal site, which was studied over 2 yr, values of density and activity of attached and total bacteria showed marked seasonal periodicity, being low in summer and high from autumn to spring. This was a consequence of density of attached bacteria being dependent on the concentration of suspended solids, which was high in winter and low during summer. Values of density and activity of free bacteria, which were less than those of attached bacteria, showed no seasonal pattern but fluctuated irregularly and were independent of concentration of suspended solids. At two further sites, which were studied over 1 yr only, the density of bacteria varied seasonally in a similar manner, but the activity of attached and total bacteria showed no distinct seasonal pattern — this may, however, be due to limitations of the data rather than to fundamental differences between the sites studied. 相似文献
6.
Phytoplankton productivity of the tidal estuaries and coastal waters of southern Nassau Country, Long Island, New York, USA was determined monthly at 28 stations during 1966. Diatoms alternated with dinoflagellates in dominating the standing crop in the coastal area. The estuaries were characterized by sustained blooms of green flagellates and dinoflagellates during the spring/summer period, 1966. Chlorophyll a ranged from 1.0 to 27.6 mg/m3 in the estuarine area, and 1.45 to 10.15 mg/m3 in adjacent coastal waters. Rate of phytosynthesis per unit weight chlorophyll a for surface samples in the region under study a veraged from 3.1 to 3.5 mgC/mg chlorophyll a/h. At light saturation, however, the ratio varied according to water temperature and species' composition. Primary productivity decreased seawards, with mean values for 1966 of 0.35, 0.22, and 0.16 gC/m3/d for the estuarine, nearshore and offshore areas, respectively.This study was carried out at the Lamont Geological Observatory of Columbia University Palisades; New York, USA.The study was conducted with financial assistance from Nassau County, New York, and the G. Unger Vetlesen Foundation. 相似文献
7.
K. Moebus 《Marine Biology》1972,13(1):1-13
During 1969 and 1970, the antibacterial activity of North Sea water was investigated at Helgoland (southern North Sea). Tests were performed with resting cells of Escherichia coli, Staphylococous aureus and Serratia marinorubra, inoculated in freshly sampled sea water used raw or after filter sterilization. Simultaneously, growth of indigenous bacterial populations which occurred in the presence, as well as in the absence (only 1970), of test bacteria was controlled. Seasonal changes in inactivation of test cells and multiplication of marine bacteria were correlated with the life cycles of several diatom species. Breakdown of phytoplankton flowerings produced the most pronounced influence on antibacterial activity of sea water. Sometimes, enhancement of kill rate could be established during growth periods of various algal species, too. From the results obtained, it is concluded that the inactivation capacity of sea water depends, to a considerable degree, on the availability of nutritive organic matter, the concentration of which changes according to phytoplankton development. Strain-specific differences in resistance to inimical action of sea water are, in part, correlated with the ability of test cells to use, and to compete with, marine bacteria for nutrients from the sea. A somewhat modified interpretation of antibacterial activity of sea water is presented. 相似文献
8.
This work, carried out within the framework of the PRISMA II project, aims at evaluating the effects of the Po River flow on primary productivity, measured by C with in situ sample incubations. A total of four cruises was carried out in late winter and early summer (1996-1998) along transects from the coast offshore. In both seasons, the highest primary productivity was found in the most oligohaline stations. The effects of the Po River inputs were therefore clearly evident in the westernmost area, both in high productivity values and in the shape of productivity profiles. In late winter, extensive blooms of Skeletonema costatum and Pseudo-nitzschia delicatissima resulted in productivity values that were sometimes higher than in early summer. In early summer 1996, greatly reduced river flow clearly influenced productivity which was much lower than in 1997. 相似文献
9.
DMSP-lyase activity in five marine phytoplankton species: its potential importance in DMS production 总被引:7,自引:0,他引:7
Activity of DMSP-lyase, which cleaves dissolved DMSP (henceforth DMSPd-lyase), was examined in five axenically cultured phytoplankton species, including both DMSP-producing and non-DMSP-producing
species. High DMSPd-lyase activity was found in two DMSP producers, Heterocapsa triquetra strain NIES-7 and Scrippsiella trochoidea strain NIES-369 (Dinophyceae). The DMS production rates at 100 nM DMSPd were 0.5 fmol cell−1 min−1 for H. triquetra and 0.3 fmol cell−1 min−1 for S. trochoidea. In a non-DMSP producer, Heterosigma akashiwo strain NIES-6 (Raphidophyceae), the DMSPd-lyase activity was not found. Two DMSP-producing Prymnesiophyceae species, Isochrysis galbana strain CCMP-1323 and Gephyrocapsa oceanica strain NIES-353, did not show any obvious activity either, in contrary to other authors' findings on Phaeocystis sp., another DMSP-producing Prymnesiophyceae species. The comparison of the DMSPd-lyase activity of the two Dinophyceae species with bacterial DMSP consumption and DMS production activity in Tokyo Bay showed
that the DMSPd-lyase activity of H. triquetra and S. trochoidea could be an important mechanism for DMS production during their blooms.
Received: 9 April 1999 / Accepted: 10 December 1999 相似文献
10.
Nucleic acids and ATP to assess microbial biomass and activity in a marine biosedimentary system 总被引:2,自引:0,他引:2
Sedimentary DNA, RNA and ATP concentrations were measured at six stations on a tidal flat in the German Wadden Sea from April
to December 1995 to determine factors controlling microbial abundance and activity. Two stations (1 and 2) were located in
a mussel bed (Mytilus edulis) in the middle of the flat, and the other four were arranged in a line following ebb tide direction. DNA and ATP concentrations
were converted into carbon equivalents using literature coefficients and considered to represent total microbial biomass and
viable microbial biomass, respectively. The difference between CDNA and CATP was considered as detrital biomass. CDNA and detrital CDNA biomass both increased from spring to summer and decreased in autumn to reach lowest values in December. The most prominent
seasonal increase was observed at the mussel bed stations and two adjacent stations. CATP biomass peaked in early spring, especially at the mussel bed stations, and showed a secondary peak in summer. Regression
analysis yielded a highly significant relation between the silt content of the sediment and CDNA and CATP. However, CDNA and CATP biomass are not correlated if silt is taken into account. The relation between silt and microbial biomass in combination
with the decrease of the silt content with distance from the mussel bed indicate the importance of mussel beds for the microbial
activity on the tidal flat, most likely through deposition of feces and pseudofeces. The low silt content and microbial biomass
in winter are attributed to resuspension events. The zero intercepts of the regressions between DNA and ATP or RNA suggest
that extracellular DNA is absent in our samples. Hence, detrital CDNA biomass in our samples probably consists of intact but dormant cells. Compared to other marine habitats the percentage of
detrital CDNA biomass on the tidal flat is quite high, i.e. up to >95% of the total CDNA. However some doubt is expressed about the validity of factors used to convert ATP and DNA to carbon. The proportion of active
biomass, expressed by the ratio CATP/CDNA being the complement of the detrital CDNA percentage shows a steady decrease with time and with diminishing distance towards the mussel beds. The role of a mussel
bed as a biosedimentary system influencing spatial and temporal trends in specific activity is discussed.
Received: 26 January 2000 / Accepted: 3 July 2000 相似文献
11.
Kinetic studies on the NADP-linked glutamate dehydrogenase (GDH) activity in 8 species of marine phytoplankton have been carried out. The pH optima, linearity of reactions, apparent energies of activation, and apparent K
m values for NADPH, NH
4
>+
and -ketoglutarate were determined. Based on these data, an accurate, sensitive enzyme assay procedure is presented which was successfully tested on shipboard during a recent cruise in an upwelling region. Preliminary studies on inhibition and activation of the NADP-linked GDH activity have also been carried out. In addition to NADP-linked GDH activity, NAD-linked GDH activity was discovered in certain species of phytoplankton. The possible physiological roles of NAD-and NADP-linked GDH enzymes are discussed.Contribution No. 911 from the Department of Oceanography, University of Washington. This research was supported by the National Science Foundation Grants GA-34165 and GX-33502. 相似文献
12.
S. Mackinson G. Daskalov J.J. Heymans S. Neira H. Arancibia M. Zetina-Rejón H. Jiang H.Q. Cheng M. Coll F. Arreguin-Sanchez K. Keeble L. Shannon 《Ecological modelling》2009
Fishing mortality and primary production (or proxy for) were used to drive the dynamics of fish assemblages in 9 trophodynamic models of contrasting marine ecosystems. Historical trends in abundance were reconstructed by fitting model predictions to observations from stock assessments and fisheries independent survey data. The model fitting exercise derives values for otherwise unknown parameters that specify the relative strength of trophic interactions and, in some instances, a time series anomaly for changes in primary production. We measured how much better or worse were model predictions when bottom-up forcing by primary production were added to top-down forcing by fishing. Searching for cross system patterns, the relative contribution of fishing and changes in primary production, mediated through trophic interactions, are evaluated for the ecosystems as a whole and for selected similar species in different ecosystems. The analysis provides a simple qualitative way to explain which forcing factors have most influence on modeled dynamics. Both fishing and primary production forcing were required to obtain the best model fits to data. Fishing effects more strongly influenced 6 of 9 of the ecosystems, but primary production was more often found to be the main factor influencing the selected pelagic and demersal fish stock trends. Examination of sensitivity to ecological and model parameters suggests that the results are the product of complex food-web interactions rather than simple deterministic responses of the models. 相似文献
13.
Tests have been carried out, both on phytoplankton cultures and in the field, on the relation between the ratio of 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU)-enhanced to normal fluorescence (F
D
/F
N
) and the specific photosynthetic rate (P/B). In all cases observed, significant linear correlations were found between these two values. Differences in this relation were observed according to species and physiological conditions. Nutrient stress that occurs in batch cultures decreases both F
D
/F
N
and P/B, while lowered light intensity has a different effect on both, increasing F
D
/F
N
and decreasing P/B. This is interpreted as indicating that F
D
/F
N
is an index of photosynthetic efficiency at different light levels and an indicator of the specific photosynthetic rate (P/B) when physiological conditions vary at a given light intensity. The practical use of DCMU-enhanced fluorometry to estimate instantaneous P/B values in the field is discussed, stressing the frequent calibrations needed, as in all in situ fluorometry studies. 相似文献
14.
Seasonal differences in the locomotor activity pattern of Podarcis sicula held in constant conditions in the laboratory were found to be associated with systematic differences in both the freerunning period of locomotor rhythms () and the circadian activity time (). In order to establish whether the pineal played a role in the control of seasonal changes in circadian parameters, we examined the effects of pinealectomy in constant conditions on the locomotor behavior of lizards displaying the bimodal activity pattern typical of summer. In most lizards pinealectomy lengthened , shortened and abolished the bimodal activity pattern. Pinealectomy induced a sudden transition from the typical locomotor behavior of summer, characterized by a marked bimodal pattern, short and long , to the typical locomotor behavior of autumn, characterized by a unimodal pattern, a long and short . These results demonstrate that the pineal plays a central role in the seasonal reorganization of the circadian system that occurs in P. sicula. 相似文献
15.
Augusto Foà Gaia Mouteforti Lucia Minutini Augusto Innocenti Cecilia Quaglieri Monica Flamini 《Behavioral ecology and sociobiology》1994,34(4):267-274
The daily pattern of locomotor activity of the ruin lizard Podarcis sicula in its natural environment changes from unimodal in spring (with only one activity peak per day) to bimodal in summer (with two well-separated activity peaks per day) and it becomes unimodal again in autumn. In order to establish whether such seasonal changes in pattern might be at least in part controlled by endogenous temporal programs, lizards were collected at different times of the year and immediately after capture their locomotor behavior was tested in the laboratory under constant temperature (29°C) and in darkness. For some individuals tested in the laboratory the locomotor pattern previously expressed in the field was known. Seasonal differences in pattern have been unequivocally found to have an endogenous component, as most lizards in constant conditions retained the locomotor pattern shown in the field during the same season. Besides, in the bimodal lizards the freerunning period of locomotor rhythms () was significantly shorter and circadian activity time (a) longer than in the unimodal ones. Altogether the data are compatible with the idea that both the interdependent changes of and a and the changes in locomotor pattern occurring seasonally in the circadian activity rhythms of P. sicula would depend on changes in the phase relationship between mutually coupled oscillators which drive these rhythms.
Correspondence to: A. Foà 相似文献
16.
Seasonal and areal changes in standing stocks of phytoplankton,zooplankton and micronekton in the eastern tropical pacific 总被引:1,自引:0,他引:1
Five standing stocks were measured together at similar latitudes and longitudes on seasonally repetitive cruises in 3 areas — western, eastern, and southern — of the eastern tropical Pacific. The stocks were chlorophyll a at 0 to 150 m depth (mg/m2), night and day zooplankton at 0 to 200 m depth (ml/1000 m3), and night crustacean micronekton and fish-pluscephalopod micronekton at 0 to 200 m depth (ml/1000 m3). The logarithms of the measurements of each stock in each area were subjected to analysis of variance with the following factors: season (2 month period), latitude, and longitude. Seasonal coverage was most comprehensive, with 7 successive periods, in the western area (approximately 16° N to 3° S latitude, 100° to 122° W longitude). Most stocks in most parts of the western area had a simple seasonal cycle of low amplitude, with a single maximum and minimum that usually differed by a factor <2; some stocks in some parts of the area exhibited no seasonal cycle; all statistically significant cycles, except for fish-plus-cephalopod micronekton, were similar in phase. In the other two areas, located broadly to the east and south of the western area, suitable measurements were made at only 2 periods (opposite seasons) of the year. There were indications of phase differences between chlorophyll a and zooplankton in the eastern area, which should be further investigated. Most standing stocks declined gradually from east to west, and were higher in known upwelling areas and areas of shoal thermocline than elsewhere. 相似文献
17.
Dark survival strategies in marine phytoplankton assessed by cytometric measurement of metabolic activity with fluorescein diacetate 总被引:5,自引:0,他引:5
F. J. Jochem 《Marine Biology》1999,135(4):721-728
Cytometric quantification of cellular fluorescence upon cleavage of fluorescein diacetate (FDA) is presented as a sensitive and rapid technique to assess phytoplankton metabolic activity during exposure to prolonged darkness of 10 to 12 d. Two distinct types of metabolic response to darkness are distinguished: Type I cells (Brachiomonas submarina, Pavlova lutheri, Chrysochromulina hirta) adapt to prolonged darkness by reducing their metabolism to a lower level of activity (∼10% of initial in P. lutheri, C. hirta, ∼0.5% in B. submarina) within few days, whereas Type II cells (Prymnesium parvum, Bacteriastrum sp., unidentified pennate diatom) continue with unchanged activity. Type I cells were able to maintain their initial cell abundance and commenced rapid cell growth upon re-illumination after 12 d of darkness. Among Type II cells, diatoms were able to maintain cell abundance and growth capacity as well, whereas P. parvum was not. Type I cells are expected to exhibit competitive advantages in environments with frequent or long dark periods. Bacterivory further supports dark survival in C. hirta. Received: 10 May 1999 / Accepted: 20 September 1999 相似文献
18.
Short- and long-term time course studies of radiocarbon accumulation in the intracellular end-products of photosynthesis (proteins, polysaccharides, lipids, small metabolites) and extracellular monomers and polymers were conducted at natural light intensity during a 24-h period in Belgian coastal waters dominated by large diatoms species in September, 1983. It is shown that carbon losses observed during the long-term incubation are due to the catabolism of reserve products (polysaccharides and lipids), which occurs both during the light and dark periods and provides carbon and energy for pursuing protein synthesis during the dark. Catabolism rates, as calculated by means of a simple mathematical model, indicate reduced rates of lipid catabolism (1–2% h-1, respectively for the light and dark periods), although polysaccharide catabolism proceeds at much higher rates, namely 20% h-1 during the light and 8% h-1 during the dark period. Assuming that protein synthesis proceeds at a constant rate during the 24-h period and that 1–3 glucan constitutes the main storage product of this diatom population, it is shown that at least 65% of the gross primary production is catabolized by the cells. From this, only 16% are mobilized for dark protein synthesis. The remaining is respired, especially during the light period. 相似文献
19.
Jamie N. Womble Gail M. Blundell Scott M. Gende Markus Horning Michael F. Sigler David J. Csepp 《Marine Biology》2014,161(6):1361-1374
Foraging theory predicts that animals will adjust their foraging behavior in order to maximize net energy intake and that trade-offs may exist that can influence their behavior. Although substantial advances have been made with respect to the foraging ecology of large marine predators, there is still a limited understanding of how predators respond to temporal and spatial variability in prey resources, primarily due to a lack of empirical studies that quantify foraging and diving behavior concurrently with characteristics of prey fields. Such information is important because changes in prey availability can influence the foraging success and ultimately fitness of marine predators. We assessed the diving behavior of juvenile female harbor seals (Phoca vitulina richardii) and prey fields near glacial ice and terrestrial haulout sites in Glacier Bay (58°40′N, ?136°05′W), Alaska. Harbor seals captured at glacial ice sites dived deeper, had longer dive durations, lower percent bottom time, and generally traveled further to forage. The increased diving effort for seals from the glacial ice site corresponded to lower prey densities and prey at deeper depths at the glacial ice site. In contrast, seals captured at terrestrial sites dived shallower, had shorter dive durations, higher percent bottom time, and traveled shorter distances to access foraging areas with much higher prey densities at shallower depths. The increased diving effort for seals from glacial ice sites suggests that the lower relative availability of prey may be offset by other factors, such as the stability of the glacial ice as a resting platform and as a refuge from predation. We provide evidence of differences in prey accessibility for seals associated with glacial ice and terrestrial habitats and suggest that seals may balance trade-offs between the costs and benefits of using these habitats. 相似文献
20.
A laboratory study was conducted to assess the impacts of diazinon, linuron or a fungicidal preparation of mancozeb supplemented with dimethomorph on dehydrogenase activity (DHA) in loamy sand (LS) and sandy loam (SL) soils with different features. The pesticides were applied at the maximum predicted environmental concentrations (PEC) under field conditions and at 5 or 100 times the PEC. More distinct effects were observed in LS than SL soils at all sampling times. For PEC, a significant decrease in DHA was found in LS soil treated with diazinon or the fungicidal preparation on day 1 and during the incubation period, respectively. However, DHA did not decrease in SL soil treated with the pesticides at this dosage. For the higher concentrations, decreased DHA was ascertained in LS soil treated with diazinon and the fungicidal preparation at all sampling times, whereas for linuron this effect was evident on days 14 and 28. By contrast, only 100-fold PEC significantly decreased DHA in SL soil amended with diazinon or linuron, compared with the fungicidal preparation that decreased DHA at five-fold PEC. Our results indicate that DHA was particularly sensitive to the fungicidal preparation in both soils, whereas linuron was less harmful than diazinon. 相似文献