活性污泥总DNA提取方法的比较

从处理某制药废水的MBR反应器中采集活性污泥,评价不同DNA提取方法对其总DNA提取效率的影响。DNA提取分细胞裂解和DNA纯化2步,对细胞裂解比较了珠磨匀浆法、反复冻融法、十二烷基磺酸钠（sodium dodecyl sulfate,SDS）裂解法等7种方法;对DNA纯化比较了酚/氯仿纯化法和胶回收纯化法。结果表明,SDS裂解法、酚氯仿纯化法最优。通过条件优化实验,确定SDS裂解酚/氯仿纯化法在污泥量1.1 g,10 000 r/min离心5 min的操作条件下,获得的DNA产量（10 774 μg/g泥重）和纯度（OD₂₆₀∶OD₂₈₀=1.84）等综合指标最好。     

氨氧化菌群的筛选及发酵条件的优化

利用选择性培养基对活性污泥进行连续驯化,筛选出氨氮去除效率较高且稳定的氨氧化菌群。采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术分析了氨氧化菌群在连续传代过程中菌群的结构差异,并对菌群的发酵培养基及发酵条件分别进行了正交优化和单因子优化。结果表明,该菌群的最佳发酵培养基为每升培养基中含碳酸盐缓冲液15mmol,硫酸铵4.2 mmol,磷酸盐缓冲液12.5 mmol,硫酸亚铁0.9μmol,氯化钙0.4 mmol,硫酸镁1.5 mmol;最佳发酵条件为:接种量为14%,装液量为60 mL/250 mL,温度为35℃。在此条件下,菌群对氨氮的去除效果较优化前提高了155%。     

高效氨氧化菌群富集、驯化及其动态变化规律分析

利用选择性培养基对氨氧化菌群进行了连续驯化,得到了氨氮去除效率稳定的氨氧化菌群。采用平板菌落计数法结合聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术考察了氨氧化菌群在连续传代过程中数量及群落结构的动态变化,并考察了pH值、碳源(HCO3-)浓度和氨氮浓度等因素对氨氧化菌群去除氨氮效率的影响。结果表明,通过连续驯化,氨氧化菌的比例由最初的1.8%提高到了31.3%。在碳源浓度为1.5 mg/L,NH4+-N初始浓度为200 mg/L,pH值为8的条件下,菌群对氨氮的去除率达99%以上。     

Influence of tetracycline exposure on the growth of wheat seedlings and the rhizosphere microbial community structure in hydroponic culture

In this study, the effects of tetracycline exposure on wheat growth and the microbial community structure in the rhizosphere were investigated under hydroponic culture conditions. Exposure to various concentrations of tetracycline resulted in significant suppression of the growth of wheat roots and shoots, with minimum doses of 0.8 mg L^?1 and 4 mg L^?1 resulting in inhibition rates of 32% and 15.4%, respectively. Complete inhibition of the growth of these two parts of wheat plants was observed in response to treatment with tetracycline at 20 mg L^?1 and 100 mg L^?1, respectively. However, the germination of wheat seeds was not sensitive to exposure to tetracycline. The effects of tetracycline exposure on the microbial community in the wheat rhizosphere were evaluated through traditional cultivation and molecular biological analyses. The cultivation results indicated that bacteria were the dominant population, being present in concentrations of 1× 10⁸–2.45× 10⁹CFUs mL^?1, although 39% to 87% inhibition occurred in response to tetracycline. The concentration of fungi increased in all tetracycline treated samples to 2.5 to 15.8 times that of the control. The highest concentration of fungi (4.27× 10⁸ CFU mL^?1) was observed in response to 60 mg L^?1 tetracycline after 15 days of cultivation. In this stage, a large amount of fungal colonies was observed on the surface of the culture solution, the wheat roots became rotted and the plants became atrophic or even died. Molecular biological analysis indicated that the bacterial community structure was significantly different in samples that were exposed to high levels of tetracycline (over 20 mg L^?1) than in samples that were exposed to lower concentrations. As the concentration of tetracycline increased, the diversity of the bacteria decreased. Additionally, several dominant sensitive species such as Sphingobacterium multivorum were suppressed by tetracycline, while some resistant species such as Acinetobacter sp. appeared or were conserved. The bacteria population tended to stabilize when the drug concentration exceeded 40 mg L^?1.     

Bacterial diversity in paclobutrazol applied agricultural soils

The aim of this study was to investigate the bacterial communities on paclobutrazol [(2RS, 3RS)-1-(4-Chlorophenyl)-4, 4-dimethyl-2-(1H-1,2,4-triazol-1-yl) pentan-3-ol]–applied agricultural soils by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR) amplified 16S rDNA gene fragments. Three different agricultural soil samples were collected from paclobutrazol applied mango and waxapple orchards, peanut fields and untreated rice fields as a control for DGGE analysis. The DGGE pattern of PCR- generated 16S rDNA gene fragments indicated that the bacterial populations from four paclobutrazol–applied soils of peanut fields were closely related to each other and two paclobutrazol–applied soils of mango and waxapple orchards harbored closely related bacterial communities. But, paclobutrazol–free agricultural soils comprised relatively a different bacterial group. However, the bacterial populations of mango and waxapple orchard are completely different from the bacterial communities of peanut field. Further purification and sequence analysis of 40 DGGE bands followed by phylogenetic tree assay showed similar results that soil bacteria from paclobutrazol applied mango and waxapple orchard are phylogenetically related. Based on the phylogenetic analysis, the clone M-4 was clad 100 % (bootstrap value) with Mycobacterium sp. The Mycobacterium sp. has been proved to degrade the phenolic compounds such as phenol, 4-chlorphenol, 2,4-dichlorophenol and paclobutrazol molecule containing chlorobenzene ring.     

Variations of Bacterial Community Structure in Flooded Paddy Soil Contaminated with Herbicide Quinclorac

The denaturing gradient gel electrophoresis (DGGE) method was applied to determine the relative genetic complexity of microbial communities in flooded paddy soil treated with herbicide quinclorac (3,7-dichloro-8-quinoline-carboylic acid). The results obtained showed a significant effect of quinclorac on the development of bacterial populations in soils contaminated with different concentrations of the herbicide at the early time after application. In general, however, the number of populations of the same soil sample treated with the same concentration of the quinclorac differed obviously with increasing incubation time within the early 8 weeks. The scale of differences in banding patterns-showed that the microbial community structures of the quinclorac-treated and non-quinclorac-treated soils were not significantly different after 21 weeks of incubation. Quantification, as demonstrated in this paper, was studied by establishing dose-response relationships. Significant pattern variations were quantified. Prominent DGGE bands were excised, cloned and sequenced to gain insight into the identities of predominant bacterial populations. The majority of DGGE band sequences were related to bacterial genera Clostridium, Sphingobacterium, Xanthomonas and Rhodococcus.     

Detection of Class I and II integrons for the assessment of antibiotic and multidrug resistance among Escherichia coli isolates from agricultural irrigation waters in Bulacan,Philippines

Contaminated irrigation water may greatly affect not only the quality of produce but also the people exposed to it. In this study, agricultural irrigation waters in Bulacan, Philippines were assessed and found to be contaminated with Escherichia coli (E. coli) ranging from 0.58 to 4.51 log₁₀ CFU/mL. A total of 79 isolates of E. coli were confirmed through polymerase chain reaction (PCR) amplifying the uidA gene and were tested for phenotypic resistance using 10 antimicrobials through the Kirby–Bauer disc diffusion method. Forty-six isolates (58.22%) were noted to be multidrug resistant (MDR) with high resistance rate to cephalothin, tetracycline, streptomycin, ampicillin, trimethoprim, nalidixic acid, and chloramphenicol. Moreover, this study also examined the prevalence of Class I and II integrons accounting to 67.39% and 17.39%, respectively, of the MDR E. coli strains using multiplex PCR. The results imply that the agricultural water used in Bulacan is contaminated with the fecal material of man or other animals present in the area, and the presence of MDR bacteria, which pose a potential threat to individuals in these areas, is alarming. In addition, detection of integrons could be a good marker for the identification of MDR isolates. Lastly, this study could develop strategies for the proper management of farming sites leading to the detection of food-borne pathogens and prevention of infectious diseases.     

产甲烷厌氧污泥降解含氮杂环化合物动力学与微生物种群的分析

研究了以焦化废水中典型的含氮杂环化合物吡啶、吲哚作为单一受试物质下,厌氧污泥的降解动力学。并通过应用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术,分析对比降解上述2种受试物质的厌氧污泥微生物多样性的差异,从分子生物学水平上阐明厌氧污泥微生物多样性的差异以及不同专性菌群的产生是导致不同受试物质下厌氧污泥降解速率快慢的主要原因之一。结果表明,在2种受试物质下厌氧污泥体系中均出现了Methanogenic prokaryote,说明上述系统中的厌氧污泥产甲烷活性良好,是产甲烷厌氧污泥。厌氧污泥对2种受试物质降解的容易程度为吡啶>吲哚。     

Detection of Escherichia coli in a cattle manure composting process by selective cultivation and colony polymerase chain reaction

Livestock manure is suitable for use as a composting material. However, various intestinal microbes, such as Escherichia coli, are significant components of such manures. Thus, it is desirable that the level of intestinal microbes, and particularly opportunistic pathogens, in compost is inspected and counted regularly. The sensitivity and specificity of detection of E. coli in compost have been improved by selective cultivation followed by colony polymerase chain reaction (PCR) using the ECO primer. Indeed, the sensitivity of this method is higher than that of DNA extraction from compost and PCR. In this study, changes in numbers of E. coli present in a field-scale composting process over time was assessed using selective cultivation and colony PCR. Numbers of ECO-positive colonies after 24 h decreased, with a concomitant rise in compost temperature. ECO-positive colonies were not detected from 33 to 48 h. However, ECO-positive colony numbers increased beginning on day 4 and continuing until day 42. Thus, it seems likely that the high temperatures reached during the composting process did not affect E. coli numbers in the final compost. Additionally, selective cultivation followed by colony PCR using specific primers is an appropriate method of determining levels of cultivable pathogens in composted materials.     

Development and Integration of Quantitative Real-Time PCR Methods for Detection of Mitochondrial DNA and Methanobrevibacter smithii nifH Gene as Novel Microbial Source Tracking Tools

A novel microbial source tracking (MST) method based on the detection of human and non-human markers was developed and applied to track the origin of fecal pollution in water systems. Mitochondrial DNA sequences were used to develop new quantitative real-time polymerase chain reaction (qPCR) assays for dog, poultry, and gull. The targets were included as part of a toolbox including human, cow, pig, and sheep assays. A primer and probe set for the detection of the human-specific nifH gene of Methanobrevibacter smithii was also designed as an indicator of human fecal contamination. The assays were tested for specificity and applied to fecal-spiked surface waters and environmental samples collected from two river catchments impacted by sources of human and non-human fecal contamination. The MST methods described were applicable to both spiked waters and environmental samples, and using the two approaches the origin of fecal pollution could be successfully determined in mixed source fecally polluted waters.     

土壤改良剂对油菜富集238U、226 Ra及232 Th的影响

通过网室盆栽试验,研究了施加磷矿粉(PR)、羟基磷矿粉(HA)、豆渣(SM)、骨碳(BC)及硫酸亚铁等土壤改良剂对油菜(Brassica oleracea L.)自铀矿区污染农田土壤吸收铀(238U)、镭(226Ra)及钍(232Th)的影响.结果表明,不同处理下油菜茎叶对238U、226Ra及232Th的富集系数分别在3.6×10-3～6.2×10-3、6.2×10-3～10.0×10-3及0.9×10-4～2.0×10-4之间.上述不同改良剂处理均能在一定程度上降低植物对238U、226Ra及232Th的吸收,以SM、HA处理效果较显著,其中SM处理下238U、226Ra及232Th的富集系数分别比对照降低42.9%、39%和71%.SM及HA对降低铀矿区污染土壤中植物对上述核素的吸收和富集具有潜在的应用价值.     

微纳米曝气对水库沉积物细菌及磷功能菌群落的影响

为探究微纳米曝气对水库沉积物细菌及磷功能菌群落的影响,以三明市东牙溪水库为研究对象,通过Illumina高通量测序,分析了曝气前后沉积物细菌、聚磷菌与有机解磷菌的群落组成、结构及其与环境因子的相关性。结果表明,微纳米曝气对底部产生充氧作用,进而对沉积物微生物的群落造成一定影响,微纳米曝气后,变形菌门(Proteobacteria)的相对丰度上升了24.7%,厚壁菌门(Firmicutes)上升了8.3%,而绿弯菌门(Chloroflexi)下降了5.9%,酸杆菌门(Acidobacteria)下降了7.0%。曝气前,沉积物中聚磷菌的优势菌属包括Syntrophus(26.79%)、Anaeromyxobacter(14.6%)、Nitrospira(12.6%)和Anaerolinea(11.3%);而曝气后,聚磷菌优势菌属则为Pseudomonas(65.1%)和Clostridium(22.9%)。有机解磷菌的优势菌属在微纳米曝气前后同样发生了改变：在曝气前,优势菌属为Azospira、Mesorhizobium、Pseudomonas、Chelatococcus、Variovorax,分别占8.6%、5.4%、5.3%、5.2%、和5.2%;在曝气后,优势菌属则为Azospira、Variovorax、Chelatococcus、Pseudomonas、Acidovorax,分别占11.3%、6.5%、6.1%、5.9%和5.8%。冗余分析结果表明,不管是聚磷菌还是有机解磷菌,其群落组成与沉积物表层的溶解氧(DO)均显著相关(P<0.05)。微纳米曝气工程的实施,对水库沉积物微生物的群落组成、结构有一定影响。     

Detection of dioxygenase genes present in various activated sludge

GOAL, SCOPE AND BACKGROUND: Activated sludge from refineries contains various microorganisms that could utilize aromatics under aerobic conditions due to the oxygenase enzymes. Dioxygenase enzymes are oxygenases, which are involved in the ring cleavage step of aromatic hydrocarbons. In this study, the selected catabolic loci involved in ring cleavage have been monitored in the activated sludge samples at different time intervals. The investigation of the dioxygenase genes in the Effluent Treatment Plants (ETPs) and evaluation of their presence at different time points provides a clue for the aromatic utilizing potential of the inherent microbial flora. METHODS: The catabolic gene loci pheB, xylE, tod-isp, bed and nahG responsible for the enzymes catechol 1,2-dioxygenase, catechol 2,3-dioxygenase, toluene dioxygenase-iron-sulphur protein component, benzene dioxygenase and naphthalene dioxygenase were used respectively. The time dependent change in eubacterial population was demonstrated by the amplification of 16S rDNA product, followed by restriction digestion. The template DNA was obtained from the activated sludge collected from ETPs. The supporting physiological data for the overall performance of sludge was developed using respirometric analysis. The on-site COD and MLSS analysis for ETP was used in final evaluation. The study was carried out with samples collected from three different ETPs and also from a selected ETP at different time intervals. RESULTS AND DISCUSSION: The respirometric studies were carried out with phenol, catechol, toluene, and naphthalene to arrive at the target genotypes for further study by PCR protocol. The respirometric analysis coupled with the COD and MLSS analysis represented the physiological capacity of the various sludges. Initially, the tracking protocol was optimized by using different sludge samples, which were collected from refineries. The selected genotypes were amplified and their presence has been confirmed using Southern analysis. The gene loci tod-isp, bed and xylE were commonly observed at various time intervals of the sludge from the same source. The gene loci pheB and nahG were found to be relatively rare. CONCLUSION: The 16S rDNA PCR products after restriction digestion produced different DNA fingerprint patterns, suggesting that the microbial community composition was diverse in the three sources. Similarly, the presence of the catechol 2,3-dioxygenase, benzene dioxygenase and toluene dioxygenase genes confirmed the aromatic degrading potential in the various sludges. The probes could not pick the nahG and pheB genes. However, the respirometeric assay suggested that the oxidative capacity to use naphthalene as a substrate exists. RECOMMENDATION AND PERSPECTIVE: Our study of the diversity at various time points from the ETP provided an overview of the shifts of the catabolic composition of the sludge. This also depends on the influential parameters like the incoming pollutant level and the environmental conditions that are prevailing and often changing from time to time. The results of direct DNA extraction and PCR amplification do reflect the relative abundance of a particular catabolic genotype, which could be used to monitor the efficiency of treatment.     

悬浮填料生物膜反应器细菌群落季相更替对处理效果的稳定作用

针对生物实验室污水处理难度高及其秋冬季节达标率低的问题,采用改进工艺的悬浮填料生物膜反应器(moving bed biofilm reactor, MBBR)进行连续处理,观察秋冬季节MBBR水质处理效果,利用高通量测序技术研究环境因子水温(Tw)、溶解氧(DO)、pH对生物膜细菌群落更替的影响以及主要微生物种群变化。结果表明,Tw由26 ℃下降到10 ℃期间,反应器COD、${{\rm{NH}}_4^ + }$-N去除率仍然分别保持在75%、80%左右,MBBR出水稳定在一级A标准。变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和浮霉菌门(Planctomycetes)是生物膜主要优势菌门,Tw的下降引起拟杆菌门的相对丰度显著升高。假单胞菌属(Pseudomonas)和黄杆菌属(Flavobacterium)在低Tw下成为优势菌属,动胶菌属(Zoogloea)相对丰度保持稳定。通过冗余分析(RDA)发现,DO与短波单胞菌属(Brevundimonas)、pH与脱氯单胞菌(Dechloromonas)、固氮弧菌属(Azoarcus)具有显著正相关性,Tw与假单胞菌属、黄杆菌属具有显著负相关性。MBBR结果揭示,细菌群落动态更替是MBBR出水水质仍然保持稳定的重要原因。     

Effects of hydrodynamics on the distribution of trace persistent organic pollutants and macrobenthic communities in Bohai Bay

In recent years, the rapid economic development along Bohai Bay, has brought out continuous increasing of the pollution loads in the Bohai Sea, especially by the large coastal reclamation project, Tianjin Binhai New Area. In the period of 2007-2009, we collected the sediments of the main rivers, estuaries, intertidal zone, and near-shore area of Bohai Bay, and Macrobenthos associated with the marine sediments to assess the influence of hydrodynamics in the coastal environment on the pattern of trace contaminants and the macrobenthic community. Based on data derived from these samples, the levels of polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) in sediments followed the order PAHs > OCPs > PCBs > PBDEs. The higher concentrations of PCBs, OCPs, PAHs and PBDEs were found in the estuarine and near-shore environment of the Dagu Drainage River. The spatial distribution of OCPs was different to that of PCBs due to the direction of the velocity field of Bohai Bay in its old and new topography, and the higher water-solubility of OCPs than that of PCBs. The results of the Pearson correlation and the PCA indicate that the medium diameter (MD) of sediments was the predominant factor influencing the distribution of PCBs and OCPs, most sampling sites were characterized mainly by TOC of sediments and biomass of macrobenthos. The results indicate that the distribution of trace contaminants and macrobenthic community in Bohai Bay are mainly affected by the hydrodynamic conditions.     

污泥施用对根际和非根际石灰性土壤中细菌多样性的影响

为研究污泥施用对石灰性土壤细菌多样性的影响,在连续2年种植夏玉米大田系统中,施用不同量的腐熟污泥,采集根际和非根际土壤进行16S rDNA-V3-V4区高通量测序,分析污泥施用对石灰性土壤细菌丰富度、多样性指数、群落结构、功能基因在代谢途径的影响,同时对细菌群落和环境因素之间的相关性进行分析。结果表明：污泥用量分别在3.5～37.5 t·hm⁻²和3.5～7.5 t·hm⁻²有利于非根际和根际土壤OTU数量增加;但污泥在75 t·hm⁻²过量施用时,根际和非根际土壤细菌的Shannon多样性指数显著降低,Simpson指数显著增加,Ace,Chao丰富度指数显著降低。主成分和门水平物种丰度分析表明：污泥施用量不同会造成根际和非根际细菌群落结构差异,并且当污泥施用量为75 t·hm⁻²时,明显降低了非根际土壤放线菌门的丰度;污泥施用量在3.75～37.5 t·hm⁻²时,非根际土壤中拟杆菌门丰度会明显增加。与对照相比,当污泥用量为75 t·hm⁻²时,根际土壤拟杆菌门有明显的增加,增加丰度达1.45倍,但酸杆菌门和放线菌门丰度降低了49.74%和80.57%。冗余分析(RDA)表明,土壤TN、Cd、Cu、TP是影响土壤细菌群落最主要的因素,其中TN、Cd、Cu和细菌Shannon、Simpson多样性指数呈现显著相关性(P< 0.05)。由此可见,连续过量施入污泥会对根际和非根际石灰性土壤中细菌多样性造成不利影响,而且上述微生物多样性变化可作为污泥合理施用的判断依据。     

印染废水处理的磁混凝-高梯度磁分离协同作用

混凝过程作为工业废水的预处理技术普遍受到重视,在混凝过程中降低污泥产生量并提高污泥分离速度是该技术发展的方向,基于上述目标,通过将磁粉引入絮体使之磁化并在自行研制的高梯度磁分离装置中实现磁混凝与磁分离的协同作用.以高浊度的印染废水作为试验废水,以色度、COD及SS作为考核指标,重点考察了磁混凝反应及磁分离的影响因素.当印染废水的色度约为900倍、COD约为595 mg/L、SS约为500 mg/L时,在pH=8.5、FeSO4500 mg/L、PAM3.5 mg/L、磁粉400 mg/L的适宜磁混凝反应条件下,相应指标去除率比传统混凝法分别高出17.3%、21.7%及24.2%,此时絮团沉降速度增大了64.3%,污泥体积减少了61%,污泥压缩比为0.39.在电流强度8 A、流速2.5 L/min和介质填充率1%的操作条件下,该磁性絮体流经高梯度磁分离装置时的水力表面负荷达到61.0 m3/m2·h,处理出水达到国家二级排放标准.     

温度对生物除磷系统微生物种群关系及动力学的影响

以西安市第三污水处理厂的氧化沟工艺为对象,利用荧光原位杂交技术（FISH）,研究了温度对强化生物除磷系统（EBPR）除磷性能及微生物种群关系的影响。结果表明：当水温低于20 ℃时,厌氧释磷速率和乙酸吸收速率随着温度上升逐渐增加,聚磷菌（PAOs）占总细菌（EUB）的比例也逐渐增加;当水温在20~25 ℃之间,厌氧释磷速率达到最大值,为10.86 mg P·（g VSS·h）-1,乙酸吸收速率随温度升高持续增加,PAOs占EUB的比例也达到最大值,为6.65%;当水温高于25 ℃时,厌氧释磷速率随温度的升高而下降,而乙酸吸收速率则继续增加,聚糖菌（GAOs）的数量已经超过PAOs而成为优势菌,导致处理效果下降。通过对实验数据的统计,得出活性污泥厌氧乙酸吸收速率的温度系数为1.018。     

温度对SBR强化生物除磷工艺除磷性能的影响

以厌氧/好氧交替运行的序批式反应器（SBR）为对象,利用荧光原位杂交技术（FISH）,研究了温度（20、25和30℃）对强化生物除磷（EBPR）的影响。结果表明,温度为20℃时,系统的磷去除率高于98%,厌氧释磷速率和好氧吸磷速率分别为55.70 mg P·（g VSS·h）-1和45.16 mg P·（g VSS·h）-1,聚磷菌（PAOs）占总细菌（EUB）的比例达到90%,而聚糖菌（GAO）的比例只有1%;温度升高到25℃后,除磷效果不断降低,释磷速率和吸磷速率逐渐下降,PAOs的比例下降,而聚糖菌（GAOs）的比例不断增加;温度为30℃时,出水水质恶化,磷去除率仅为67%,释磷速率和吸磷速率分别为33.66 mg P·（g VSS·h）-1和17.55 mg P·（g VSS·h）-1,GAOs的比例高达87%,而PAOs的比例仅为5%,在与PAOs的竞争中,GAOs处于优势,导致除磷效果降低。     

pH、盐度对微生物还原硫酸盐的影响研究

采用厌氧生物处理工艺,研究了在不同盐度下pH连续降低对硫酸盐还原和有机物去除率的影响。实验结果表明,硫酸盐还原菌有很强的适应pH变化的能力,在pH值达到4以下仍有60％的硫酸盐去除率。NaCl浓度由4g／L增加到50g／L抑制了各厌氧菌的活性,导致硫酸盐和有机物去除率的下降,但硫酸盐还原菌耐受性高于产甲烷菌等其他厌氧菌,在NaCl浓度为50g／L下,硫酸盐去除率能达到50％,而有机物去除率则低于30％。qRT—PCR表明了系统菌落中SRB随着环境的变化情况与化学指标结果相一致,该反应器体系中SRB在整个厌氧菌群落中只占了很小部分。