Physiological energetics of the intertidal sea anemone Anthopleura elegantissima

Energy budgets were calculated for individuals of the sea anemone Anthopleura elegantissima (Brandt), collected in 1981 and 1982 from Bodega Harbor, California, USA. Rates of ammonium excretion were measured in high-and low-intertidal, symbiotic and aposymbiotic sea anemones within 24 h of collection. Among symbiotic and aposymbiotic individuals, no differences in excretion rate were found on the basis of intertidal height. However, rates of ammonium excretion in aposymbiotic anemones (2.14 mol NH ₊ ⁴ g^-1 h^-1) were significantly higher than in symbiotic ones (0.288 mol NH ₊ ⁴ g^-1 h^-1). Rates of excretion were used with estimated rates of oxygen uptake to calculate nitrogen quotients (NQ). NQ and RQ values from the literature were used to calculate an oxyenthalpic equivalent [501 kJ (mol O₂)^-1 for R+U], and mass proportions of protein (54%), carbohydrate (44%) and lipid (2%) catabolized during routine metabolism in this species 24 h after feeding. Integrated energy budgets of these experimental anemones were calculated from data on ingestion, absorption and growth, and estimates of translocated energy from the symbiotic algae. Contribution of zooxanthellae to animal respiration based on translocation=90% and RQ=0.97 are 41 and 79% in high-and low-intertidal anemones, respectively. Calculated scope for growth is greater than directly measured growth in both high-and low-intertidal individuals. The deficit, estimated as 30% of assimilated energy in high-intertidal anemones, is attributed to unmeasured costs (specific dynamic effect) or production (mucus). Low-intertidal anemones lost mass during the experiment, implying that the magnitude of the deficit was greater in these anemones than in upper intertidal individuals. Anemones from both shore levels lost zooxanthellae during the experiment, which contributed to energy loss since the contribution of the zooxanthellae is greater in low-intertidal anemones. Scope for growth is preserved in high-intertidal anemones (29% of assimilated energy) because metabolic demands are lower due to aerial exposure, and prey capture rate is higher compared to lowshore anemones. Although possibly underestimated, lower scope for growth in low-shore anemones may result from continuous feeding and digestion processes that are less efficient than those of periodically feeding high-intertidal anemones.     

Effects of starvation,and light and dark on the energy metabolism of symbiotic and aposymbiotic sea anemones,Anthopleura elegantissima

Rates of oxygen and carbon-dioxide exhange were measured in symbiotic and aposymbiotic specimens of the sea anemone Anthopleura elegantissima while fed and starved under light or dark conditions. Respiratory quotients indicated that fed anemones switched from a carbohydrate to a fat catabolism when starved, with the exception that symbiotic individuals starved in the light showed a pronounced carbohydrate catabolism for over 1 month. The source of the carbohydrate was probably photosynthate translocated by the dinoflagellate Symbiodinium (=Gymnodinium) microadriaticum (Freudenthal) living in the anemones' tissues. The starved symbiotic anemones maintained in the light had lipid levels not significantly different from fed controls and 44 to 61% higher than starved aposymbiotic anemones after 1 month. Thus, the quality and quantity of the metabolic flux from the symbionts to the sea anemone were sufficient to conserve the host's lipid reserves.     

Effect of feeding regime and irradiance on the photophysiology of the symbiotic sea anemone Aiptasia pulchella

To determine how the animal and algal components of the symbiotic sea anemone Aiptasia pulchella respond to changes in food availability and culture irradiance, sea anemones from a single clone were maintained at four irradiance levels (320, 185, 115, and 45 E m^-2 s^-1) and either starved or fed for 5 wk. Changes in protein biomass of sea anemones maintained under these conditions were not related to the productivity of zooxanthellae, since the protein biomass of fed A. pulchella decreased with increase in irradiance and there was no difference in protein biomass among starved sea anemones at the four irradiance levels. Except for the starved high-light sea anemones, the density of symbiotic zooxanthellae was independent of culture irradiance within both starved and fed. A. pulchella. Starved sea anemones contained over twice the density of zooxanthellae as fed sea anemones. Within both starved and fed individuals, chlorophyll per zooxanthella increased with decreasing culture irradiance while algal size remained constant (in fed sea anemones) at about 8.80 m diameter. Chlorophyll a: c ₂ ratios of zooxanthellae increased with decreasing culture irradiance in zooxanthellae from starved sea anemones but remained constant in zooxanthellae from fed sea anemones. As estimated from mitotic index data, the in situ growth rates of zooxanthellae averaged 0.007 d^-1 and did not vary with irradiance or feeding regime. Photosynthesis-irradiance (P-I) responses of fed A. pulchella indicated an increase in photosynthetic efficiency with decreasing culture irradiance. But there was no consistent pattern in photosynthetic capacity with culture irradiance. Respiration rates of fed sea anemones also did not vary in relation to culture irradiance. The parameter I _k , defined as the irradiance at which light-saturated rates of photosynthesis are first attained, was the only parameter from the P-I curves which increased linearly with increasing culture irradiance. The daily ratio of net photosynthesis to respiration for A. pulchella ranged from 1.6 to 2.8 for sea anemones maintained at the three higher irradiances, but was negative for those maintained at 45 E m^-2 s^-1. Since the final protein biomass was greatest for sea anemones maintained at the lowest irradiance, these results indicate that sea anemone growth cannot be directly related to productivity of zooxanthellae in this symbiotic association.     

The effects of symbiotic state on heterotrophic feeding in the temperate sea anemone <Emphasis Type="Italic">Anthopleura elegantissima</Emphasis>

The temperate sea anemone Anthopleura elegantissima is facultatively symbiotic with unicellular algae. Symbiotic A. elegantissima can supplement heterotrophic feeding with excess photosynthate from their algal partners, while asymbiotic individuals must rely solely on heterotrophy. A. elegantissima individuals were collected from Swirl Rocks, Washington (48°25′6″ N, 122°50′58″ W) in July 2010, and prey capture and feeding characteristics were measured to determine whether asymbiotic individuals are more efficient predators. Feeding abilities were then measured again after a 3-week exposure to full sunlight or shaded conditions. Freshly collected asymbiotic anemones had larger nematocysts, but symbiotic individuals showed greater nematocyte sensitivity. Sunlight enhanced digestion and reduced cnida density in all anemones regardless of symbiotic state. Results suggest that the phototropic potential of A. elegantissima, as influenced by symbiotic condition, has little effect on heterotrophic capacity. The anemones appear to maximize heterotrophic energy input independent of the presence or identity of their algal symbionts.     

Effects of feeding rate on conversion efficiency and chemical composition of the fish Tilapia mossambica

Average daily rations of 14, 35 or 58 mg Tubifex tubifex worm per gram of the fish Tilapia mossambica Peters represent maintenance, optimum or maximum feeding levels. At these levels, conversion efficiency (K ₁) is 5,9 or 24%. An amount of 65 mg worm/g fish/day, when fed under experimental conditions, is converted with the poorest conversion efficiency (4%). Test individuals fed at 11 to 49 mg worm/g fish/day show a decreasing trend in water content (78.1 to 74.8%), and an increasing trend in fat content (32.1 to 44.2%). Below or above this feeding rate range, water content increases, while fat content decreases. The range of individual variations in fat content is nearly 3 times greater than that of ash and 15 times greater than that of water. Test individuals starved for 60 days lose 2.1 mg dry body weight/g/day. This loss is contributed by calorifically equivalent amounts of fat and protein. The endogenous loss of nitrogen by these individuals averaged 0.18 mg N/g body weight/day.     

Experimental prey species preferences of Hexaplex trunculus (Gastropoda: Muricidae) and predator–prey interactions with the Black mussel Mytilus galloprovincialis (Bivalvia: Mytilidae)

Hexaplex trunculus is one of the most widespread Mediterranean species of muricid gastropod and lives on rocky, sandy-mud and mud substrata. Although common in the Adriatic Sea, relatively little is known about its ecology especially feeding behaviour. The aim of this study was to explore the aspects of the feeding behaviour of H. trunculus using Arca noae, Modiolus barbatus and Mytilus galloprovincialis as experimental prey. Prey species preference, predator size, prey size choice, feeding rates, handling times and mode and place of attack were analysed. Typically, only M. galloprovincialis was attacked: A. noae rarely at the byssal gape and M. barbatus never. Small (40 mm) H. trunculus could not easily attack large M. galloprovincialis (65 mm) and preferred small (20 mm) and medium (35 mm) sized prey. Conversely, medium (55 mm) and large (70 mm) H. trunculus fed randomly on M. galloprovincialis of all three sizes. The feeding strategy adopted by H. trunculus individuals varied with respect to size. A tendency to drill the prey shell was recorded for small predators, whereas marginal chipping was adopted more frequently by large individuals. On average small, medium and large H. trunculus consumed 2.4±1.6 (range 0–4), 1.2±1.6 (range 0–4) and 2.0±2.1 (range 0–6) M. galloprovincialis, respectively. There was a statistically significant difference in prey-handling time with respect to the method of access adopted, predator and prey sizes. The time required to access a M. galloprovincialis individual by marginal chipping was considerably less than that required for drilling. H. trunculus consumed an average of 0.60±0.80 g M. galloprovincialis tissue dry weight over a 5-week period, that is, ~40% of its own tissue body weight. This translates to an average-sized (55 mm shell height) H. trunculus consuming ~18 M. galloprovincialis of 50 mm shell length (minimum marketable size) per year. H. trunculus showed no preference to drill either the left or right valves of M. galloprovincialis but generally attacked the posterior shell margin.     

Host feeding and nutrient sufficiency for zooxanthellae in the sea anemone Aiptasia pallida

Nutrient sufficiency of zooxanthellae in the sea anemone Aiptasia pallida cultured in low nutrient seawater depends on the availability of particulate food to the host. Zooxanthellae in anemones unfed for 20 to 30 d exhibited the following characteristics of nutrient deficiency: cell division rates decreased; chlorophyll a content gradually decreased from 2 to <1 pg cell^–1; and C:N ratios increased from 7.5 to 16. Over a 3-mo period, algal populations in unfed anemones gradually decreased, indicating that zooxanthellae were lost faster than they were replaced by division. The mitotic index of zooxanthellae in unfed anemones was stimulated either by feeding the host or by the addition of inorganic N and P to the medium. Whether algae are nutrient-limited in hosts under field conditions has not been examined fully; however, C:N ratios in zooxanthellae from field-collected hosts are slightly higher (9.4 vs 7.5) than in hosts fed to repletion in laboratory cultures. This observation might indicate N limitation in the field.     

Feeding and predator-avoidance by the rose anemone Urticina piscivora

We investigated the influence of feeding rate on the behavior of the rose anemone Urticina piscivora. We predicted that anemones would travel less and be less willing to detach from the substrate in response to contact with a predator (Dermasterias imbricata) as feeding rate was experimentally increased. As predicted, anemones receiving 0 mussels (Mytilustrossulus) daily moved significantly more than those receiving either 1 or 2 mussels daily. Anemones receiving 0 mussels daily also detached significantly quicker in response to predator contact compared to the 1 mussel per day group, which in turn detached significantly quicker than did the 2 mussels per day group. A field test of the same prediction gave mixed results. We also examined whether anemones could detect feeding rates of neighbors. Movement rates of anemones receiving 1 mussel daily when neighbors received 0 mussels daily were compared with those when neighbors received 2 mussels daily. We predicted that if the subjects could detect feeding rates of neighbors, they should move less when neighbors received less food than themselves (since their position is relatively good) than when neighbors received more food. The results support this interpretation. Received: 21 August 1996 / Accepted: 22 October 1996     

Effects of food ration and feeding regime on growth and reproduction of the sea urchin Strongylocentrotus droebachiensis

To determine the effects food ration and feeding regime on growth and reproduction of Strongylocentrotus droebachiensis (Müller), sea urchins in laboratory aquaria were fed kelp (Laminaria longicruris) supplied at either a high (H, ad libidum daily) or a low (L, ad libidum 1 d wk⁻¹) ration in two successive 12-wk intervals during the reproductive period. After 24 wk, urchins fed the high ration continuously (HH) or for the last 12 wk only (LH) had a significantly greater mean gonad index [(gonad weight/total body weight) × 100] and body weight than urchins fed the low ration continuously (LL) or for the last 12 wk only (HL). Urchins in the HL treatment had a significantly greater gonad index than those in the LL treatment; there was no significant difference in gonad index between the LH and HH treatments. Females had a greater gonad index than males in the low ration (LL and HL) treatments at the end of the experiment; there was no significant difference between sexes in the high ration (LH, HH) treatments. Gametogenesis proceeded to maturation in all treatments and some individuals spawned at the end of the experiment. Females in the high ration (HH and LH) treatments had a greater proportion of nutritive phagocytes in their ovaries than females in the low ration treatments, but there was no effect of feeding treatment on oocyte or ovum size. Feeding treatment had no effect on the relative abundance of nutritive phagocytes in the testes, although the proportion of spermatocytes was higher (and that of spermatozoa lower) in the high ration than in the low ration treatments. Urchins in the high ration treatments had a lower mean jaw height index [(jaw height/test diameter) × 100] and greater mean test diameter than those in the low ration treatments at the end of the experiment, although these differences were not statistically significant. Feeding rate on kelp at the end of the experiment was significantly greater for urchins in the low ration than in the high ration treatments. Our experimental results show that even relatively low rations of kelp support somatic and gonadal growth in S. droebachiensis. Increasing the supply of kelp, particularly during the period of active gametogenesis, results in maximal rates of growth and reproduction. These results suggest that populations of S. droebachiensis in barrens may derive a substantial proportion of their nutrition from drift kelp, which may contribute to their persistence in these habitats. They also explain the large body size, high reproductive effort and fecundity of urchins grazing on kelp beds. These findings have important implications for understanding the dynamics of natural populations of S. droebachiensis and for development of effective aquacultural practices. Received: 17 February 1997 / Accepted: 5 March 1997     

Host selection,location, and association behaviors of anemonefishes in field settlement experiments

Nine anemonefish species were reared in the laboratory, and individuals were released in the field (Lizard Island, Australia and Madang, Papua New Guinea) at different distances and orientations away from natural (anemone species the fishes are found with in nature) and unnatural species of host anemones. Experiments were conducted to examine factors that could affect the settlement behaviors of the fishes: current velocity, distance and orientation of the fishes to the anemones, chemical vs visual cues, and presence of conspecific or heterospecific fishes. The fishes were usually attracted toward natural host species of anemones but not towards unnatural host anemone species nor to pieces of dead coral. Host selection during settlement provided the best explanation for the host specificity patterns displayed by anemonefishes in nature. The fishes used chemical cues released from the anemones to identify and locate the appropriate host species and could effectively locate the anemones from a maximum distance of 8 m downstream. Fishes released upstream or to the side of anemones (where anemone chemicals were assumed to be reduced or absent) were much less successful in locating anemones. The ability of the fishes to locate natural host anemones was strongly reduced when there was no water current. The presence of resident anemonefishes on host species of anemones did not influence the attraction behavior of anemonefishes released downstream from the anemones. Once the released fishes got close to or entered the anemones, the resident fishes would generally bite and chase them until the recruits left the anemones. Most fishes were not stung upon initial contact with the anemones.     

Predator shoaling moderates the confusion effect in blue-green chromis,Chromis viridis

Summary In experiments, blue-green chromis [Chromis viridis (Cuvier 1830)] were fed on either scattered or aggregated swarms of brine shrimp (Artemia sp.). Ten runs with each prey dispersion treatment were performed with shoals of one, two, five and ten chromis. The mean lag in reaching peak feeding rate for fish fed on aggregated prey was significantly shorter in the larger chromis shoals. In contrast, with the scattered treatment all such lags were similar and very short. As foraging proceeded, higher feeding rates were observed in the larger feeding shoals, regardless of prey dispersion. Prey capture success (i.e. the rate of retention of intercepted prey) declined with time, but was significantly higher in groups of ten fish. Two main conclusions emerge. Firstly, grouping facilitated initiation of feeding by individuals preying on concentrated swarms and reduced the delay in reaching a maximum feeding level. This may have been due to a suppression of the confusion effect through reduced reliance upon vigilance. Secondly, reduced vigilance allowed larger shoals of chromis to feed effectively over more extended periods. Trends of increasing shoal cohesion and decreasing prey retention rate with time were consistent with a postulated increase in antipredator vigilance with declining feeding motivation.     

Foraging tactics of an ambush predator: the effects of substrate attributes on prey availability and predator feeding success

The foraging sites selected by an ambush forager can strongly affect its feeding opportunities. Foraging cane toads (Rhinella marina) typically select open areas, often under artificial lights that attract insects. We conducted experimental trials in the field, using rubber mats placed under lights, to explore the influence of substrate color and rugosity on prey availability (numbers, sizes, and types of insects) and toad foraging success. A mat's color (black vs. white) and rugosity (smooth vs. rough) did not influence the numbers, sizes, or kinds of insects that were attracted to it, but toads actively preferred to feed on rugose white mats (50% of prey-capture events, vs. a null of 25%). White backgrounds provided better visual contrast of the (mostly dark) insects, and manipulations of prey color in the laboratory showed that contrast was critical in toad foraging success. Insects landing on rugose backgrounds were slower to leave, again increasing capture opportunities for toads. Thus, cane toads actively select backgrounds that maximize prey-capture opportunities, a bias driven by the ways that substrate attributes influence ease of prey detection and capture rather than by absolute prey densities.     

Feeding ecology of a size-structured predator population,the South American sun-star Heliaster helianthus

Patterns of feeding in a population of Heliaster helianthus (Lamarck), a common and dominant species of starfish indigenous to the Pacific South American coast, were investigated in an intertidal habitat in central Peru from October 1986 to April 1987. The H. helianthus population comprised individuals of 3.5 to 30.2 cm body size (diameter) with two modal size classes. The number of rays ranged between 18 and 40, and individuals with 31 to 33 rays accounted for ca. 42% of the total population. There was a higher rate of increase in ray number with body size amongst small individuals(<13.0 cm diam). H. helianthus is capable of feeding on more than one prey item at a time (average of 5.6 to 13.2 prey items handled, with several predators observed to hold >100), and both the number of prey individuals captured and the total prey biomass were significantly correlated with predator size. Amongst a total of 1132 feeding observations, the largest number of predators (an average of 85.4% of those feeding) were preying on the mussel Semimytilus algosus whilst another mussel, Perumytilus purpuratus, ranked second with 21,9% of predators feeding. The proportion of S. algosus in the diet increased from 65.4% in the smallest predator size-group (10.9 cm diam) to 91.2% in the largest (19.0 cm). In contrast, P. purpuratus and barnacles were more highly represented in the diet of small H. helianthus. The smallest size-group (10.9 cm) had low dietary overlap with larger sizes and less specialized prey utilization. Two geographically separated populations of H. helianthus in Peru and Chile showed contrasting patterns of prey utilization. S. algosus and P. purpuratus comprised 85.5 and 6.5% by number in the diet of the Peruvian population, respectively, whilst corresponding figures for the Chilean population were 8.3 and 60.5%, with barnacles attaining a higher share (22.6%). However, the total number of prey individuals per feeding predator was almost the same in Peru and Chile, with 10.0 and 10.7 individuals, respectively. H. helianthus individuals of different sizes occupy slightly different microhabitats within the intertidal area, which, coupled with differential spatial distribution of prey species, results in the predator population being able to utilize a wide range of resources.     

Plant-derived visual signals may protect beetle herbivores from bird predators

Insect herbivores often use chemical signals obtained from their food plants to deter enemies and/or attract sexual partners. Do plant-based visual signals act similarly, i.e., repel consumers' enemies and appeal to potential mates? We explored this question using the pollen-feeding beetle Pygopleurus israelitus (Glaphyridae), a specialized pollinator of Anemone coronaria's chemically defended red-morph flowers. We presented dead beetles, which had fed either on anemones or on cat food, to young domestic chicks on a red (anemone-like) or a green (leaf-like) background. We determined whether the beetles' background color and diet affected the chicks' feeding. Cuticle surface extracts from anemone-fed beetles, but not from cat food-fed beetles, contained a secondary metabolite characteristic of anemones. Latencies to the first picking up and consuming of beetles from green backgrounds were shorter than of beetles from red backgrounds. The picking up order of beetles also indicated that prey from the green background was preferred. The chicks retained this preference when re-tested, 3 days later. Handling times of anemone-fed beetles were longer than of cat food-fed beetles. A previous study showed that glaphyrids improve their mate-finding prospects by orienting to large red anemone flowers. Here, female beetles preferred cat food-fed to anemone-fed males in mate-choice assays, thus anemone-derived chemicals did not increase mating success. Instead, the combined results indicate that A. coronaria's red flowers provide a visual signal that may both deter its herbivore's predators and attract its mates. To our knowledge, this is the first experimental evidence for a potential protective role of plant-derived visual signals for insect herbivores/pollinators.     

Sexual and asexual reproduction of Anthopleura dixoniana (Anthozoa: Actiniaria): periodicity and regulation

Reproduction of the sea anemone Anthopleura dixoniana (Haddon and Shackleton) from the high intertidal zone of southern Taiwan (120°41 E; 22°01N) was studied from April 1987 through March 1989. A. dixoniana spawns once a year, in July, and divides asexually by longitudinal fission throughout the year, with a peak in July. During the spawning season, sea anemones>3 mm pedal dise diameter can be sexed, and display a 1:1 sex ratio. Dividing sea anemones are significantly larger than non-dividing individuals, and increase in body size before fission. Under laboratory conditions, individuals kept at 28 C and fed had larger oocytes and a higher division rate than those kept at 18, 22, 25 or 32°C or starved. The division rate significantly influenced the oocyte diameter. The present study revealed for the first time, that a long photoperiod (14 h hight:10 h dark) significantly enhances the growth of oocytes in A. dixoniana under laboratory conditions.     

Chemische tarnung. Die stoffliche grundlage der anpassung von anemonenfischen an riffanemonen

Dissolved amino acids (³H-phenylalanine, ³H-proline) were accumulated and incorporated into proteins and glycoproteins by sea anemones. On objects (glass rods, pipe cleaners or filter paper) which had touched labelled anemones, tritiated substances could be detected. The mucus of labelled anemones was analysed by disc-electrophoresis. Tritium activity was found in the bands (stained by amino black). Anemone fishes adapted to labelled anemones had 4 times more tritium activity on their surface than control fishes. Most radioactivity was found on those regions which made closest contact with the anemones. The mucus of anemone fishes adapted to labelled anemones was also analysed by disc-electrophoresis. The analysis revealed protein-containing fractions in which ³H-amino acids were incorporated. The pattern of tritium activity in disc-electropherograms from labelled anemones corresponds to that of fishes adapted to those anemones. Anemones produce specific substances which influence the discharge of their nematocytes. These substances have special functions in the normal behaviour of the anemones. The substances provide protection against self-nettling and prevent the discharge of nematocytes into nearby objects which the tentacles continuously contact. The production of these substances by anemones is completely separate from the association with anemone fishes. The fishes thus simply exploit a mechanism existing independently in the anemones. Therefore, it is possible to form, in an aquarium, unnatural associations between anemone fishes and anemone species which never live in association with fishes in their natural biotope. On the basis of previous information, as well as on these new data, it is possible to develop a model which explains the control of nematocyte discharge: Substances with inhibitory qualities (protecting substances) are produced by the anemones themselves, and de-sensitize the sensory inputs of the anemones (nematocytes and sensory cells). Sensitization takes place as soon as the anemones come into contact with “stimulating substances”. This happens if anemones are touched by food objects or by anemone fishes which have been previously isolated from anemones. The surfaces of these fishes are not impregnated with protecting substances. Adapted anemone fishes, neighbouring anemones of the same species and other “adapted” objects are coated with the inhibitory substances and thus do not induce nematocyte discharge.     

Modeling the effect of daily ration and feeding frequency on growth of redtail shrimp Penaeus penicillatus (Alock) at controlled temperatures

Obtaining optimal growth of redtail shrimp at an economic production cost is essential. An experiment was conducted to evaluate simultaneous effects of daily ration and feeding frequency on growth of redtail shrimp at controlled temperatures. Two series were operated contemporarily, one at 25°C and the other at 30°C. the three daily rations were 5, 15, and 25% of fresh body weight in association with three levels of daily feeding frequency: one, three, and five times. Each series lasted for 80 days. The growth rate and the combined effects were studied on day 40 and day 80 for each series.Response surface analyses showed that, at 25°C, two quadratic polynomial equations could describe the daily growth rates (G₁ and G₂) during growth periods 1 (day 1-day 40) and 2 (day 41-day 80). The optimal daily ration and feeding frequency in terms of maximum growth rate were found at 25°C to be 22.38% of fresh body weight with three feedings/day for growth period 1 and 22.68% of fresh body weight with three feedings/day for growth period 2.At 30°C, two dynamic models were derived for each growth period that included growth optimums of 21.94% and four feedings/day for the first growth period, and 21.27% and four feedings/day for the subsequent growth period.     

Prey ecology and behaviour affect foraging strategies in the Great Cormorant

The fine link between a particular dive pattern and a specific prey item represents a challenging task in the analysis of marine predator–prey relationships. There is growing evidence that prey type affects diving seabirds’ foraging strategies, dive shapes and underwater activity costs. This study investigates whether a generalist diver, the Great Cormorant Phalacrocorax carbo, modifies the time budget allocated to prey-capture behaviour and breathing strategies (reactive vs. anticipatory) with respect to the prey type (pelagic vs. benthic). Video recordings of 91 Great Cormorants show how the ecology and behaviour of their main prey, Mullets (Mugilidae) and Flounders Platichthys flesus, affect dive/surface durations and the diving pattern. The demersal habit and the low mobility of Flounders leads to an easy access to prey with an anticipatory strategy. Moreover, the patchy distribution of this fish species increases prey-capture rates. Conversely, Mullets exploit the whole water column and are highly mobile, and this is reflected in the need of performing two sequential dives to capture a prey, both longer and likely more expensive, with a consequent switch of strategy from reactive in the searching phase to anticipatory breathing during prey-capture events. This study provides evidence that a generalist diver may switch between different foraging strategies, and it shows how each of them may be optimal under particular ecological conditions. These constraints influence the dynamics that operate within the marine food chains and have relevant implications in managing lagoon areas, including fish ponds.     

Oxygen uptake and comparative energetics among eggs and larvae of three subtropical marine fishes

Oxygen uptake was measured and energy budgets were determined for eggs and larvae of bay anchovy (Anchoa mitchilli), sea bream (Archosargus rhomboidalis) and lined sole (Achirus lineatus). Using an energetics model, both minimum rations and prey levels were estimated. The Q_{O 2} [μl (mg dry wt)^-1 h^-1] increased approximately five-fold for all species during development from egg to feedingstage larvae. The weight exponents in power functions, R=aW^b, relating oxygen uptake (R) to dry weight (W) did not differ significantly among species. The exponents were: bay anchovy, 0.9770; sea bream, 0.8382; lined sole, 0.9416. Oxygen uptake was generally lower for bay achovy than for the other species. In the energy budgets issimilation efficiencies of all species were low (24–75%). Gross growth efficiencies ranged from 11–41%, and net growth efficiencies from 38–57%. An estimated 32–83% of ingested energy was excreted in feces and urine. The lavae used a relatively small proportion of ingested energy in metabolism (6–31%). The anchovy had the lowest assimilation and gross growth efficiencies, and the highest excretion rate. Consistent with the better growth and survival observed in previous experiments, sea bream were the most efficient assimilators and had the lowest required minimum rations. Required minimum prey levels for first-feeding larvae ranged from 400 copepod nauplii l^-1 for sea bream and bay anchovy to 1 000 l^-1 for lined sole. The estimated required prey levels were higher than levels at which significant survival had been observed.