Habitat-induced morphological variation influences photosynthesis and drag on the marine macroalga <Emphasis Type="Italic">Pachydictyon coriaceum</Emphasis>

Photosynthesis, growth, distribution, and persistence of macroalgae are determined in part by the physical environment in which they live. Therefore, discerning how macroalgae interact with their physical environment is necessary to better understand their physiological performance. The purpose of this study was to examine what photosynthetic and hydrodynamic costs and benefits the morphology of Pachydictyon coriaceum (Phaeophyta) confers on the thallus in a given environment. Principal components analysis of morphometric measurements of Pachydictyon coriaceum from different flow habitats and depths separated thalli into three distinct morphs: shallow wave-exposed, shallow wave-protected, and deep. To test the hypothesis that thallus morphology affects net photosynthesis (NP), thalli of three morphotypes of P. coriaceum were incubated in an enclosed recirculating flume under three simulated light/water flow environments representing conditions from which the three morphotypes were collected. The wave-protected and deep morphs had significantly higher rates of photosynthesis than the wave-exposed morph for all three simulated environments. The dense, compact shape of the wave-exposed morph readily streamlines with flow and in doing so, potentially shades many of its internal blades likely accounting for its lower biomass-specific NP. Drag coefficients (C _d) were estimated for the three morphotypes over a range of flow velocities between 0.08 and 0.47 m s⁻¹. At lower water flow velocities (0.08–0.21 m s⁻¹), wave-exposed morphs had the lowest C _d among the three morphotypes. But drag coefficients of the three morphotypes converged with increasing flow velocities, and at velocities >0.31 m s⁻¹ there were no differences in C _d among the three morphotypes. The results of this study indicate that the environmentally-shaped morphs influence photosynthesis and, to a lesser degree, hydrodynamic forces acting on P. coriaceum.     

Flow,flapping, and photosynthesis ofNereocystis leutkeana: a functional comparison of undulate and flat blade morphologies

A number of species of macroalagae possess a flat, strap-like blade morphology in habitats exposed to rapidly-moving water whereas those at protected sites have a wider, undulate blade shape. We have explored the functional consequences of flat, narrow vs. wide, undulate blade morphologies in the giant bull kelpNereocystis luetkeana. Our study focused on the behavior of blades in ambient water currents and the consequences of that behavior to breakage and to photosynthesis. In flowing water, the narrow, flat blades flap with lower amplitude and collapse together into a more streamlined bundle than do wide, undulate blades, and hence experience lower drag per blade area at a given flow velocity. If the algae at current-swept sites had ruffled blades, drag forces would sometimes be sufficient to break the stipes. However, flat blades in a streamlined bundle experience more self-shading than do undulate blades, which remain spread out in water currents. Thus, there is a morphological trade-off between reducing drag and reducing self-shading. Photosynthetic¹⁴C-HCO₃ uptake rates decrease in slow flow when the boundary layer along the blade surface across which diffusion takes place is relatively thick. However, blade flapping, which stirs water near the blade surface, enhances carbon uptake rates in slow water currents for both the undulate and the flat morphologies.     

Morphological variation and phenotypic plasticity of buoyancy in the macroalga Turbinaria ornata across a barrier reef

Many aspects of morphology of benthic algae (length, surface area-to-volume ratio, and blade undulation) are plastic traits that vary in response to physical factors (such as light or water flow environment). This study examines whether frond buoyancy is a plastic trait, and whether differences in morphology including buoyancy affect the potential persistence of macroalgae in habitats characterized by different water flow regimes. Fronds of the tropical alga Turbinaria ornata in protected backreef environments in Moorea, French Polynesia possess pneumatocysts (gas-filled floats) and experience positive buoyant forces, whereas fronds in wave-exposed forereef sites either lack pneumatocysts entirely or have very small, rudimentary pneumatocysts and experience negative buoyant forces. Forereef fronds transplanted to the backreef developed pneumatocysts and experienced increased buoyant force indicating that buoyancy is a phenotypically plastic trait in T. ornata. In comparing the potential for dislodgement by drag, drag was greater on forereef fronds at low flow speeds as these fronds were stiffer and did not bend over at low flow speeds and therefore were less streamlined in the flow than backreef algae, which bent easily. The environmental stress factor (ESF) (a measure of the likelihood of detachment for a frond in its habitat) was higher for forereef than backreef fronds at all flow speeds. When examined with respect to the flow velocities likely in their respective habitats however, the chance of detachment for backreef and forereef was similar. Neither backreef nor forereef fronds were predicted to break under normal, non-storm conditions, but both were predicted to break in storms. Strong forereef morphologies are well suited to habitats characterized by rapid flow, whereas the weaker, buoyant, tall backreef fronds are well suited to habitats where crowding and shading is common but hydrodynamic forces are low.     

Numerical simulation for impacts of hydrodynamic conditions on algae growth in Chongqing Section of Jialing River, China

Hydrodynamic conditions are important factors for planktonic algae growth, through introducing two parameters which express the optimal velocity and the velocity range for planktonic algae growth, a new velocity factor was put forward for the formula of growth rate. Therefore, the two-dimensional unsteady ecological dynamic model for algae growth was established to analyze the effects of hydrodynamic conditions on algae growth in Chongqing Reach of Jialing River in China. The temporal and spatial distribution of Chlorophyll-a (Chl-a) concentration was simulated numerically for various water levels, under climate conditions in period of high frequency for algae blooms of Three Gorges Reservoir and nutrition status at present in the research reach. The corresponding locations and areas of likely algae blooms were analyzed and forecasted. The results showed that about 0.04 m s⁻¹ was the optimal velocity for algae growth, and the occurrence of algae blooms in large scale is almost impossible because of relatively high water flow velocity for Jialing River.     

Diffusive boundary layers and photosynthesis of the epilithic algal community of coral reefs

The effects of mass transfer resistance due to the presence of a diffusive boundary layer on the photosynthesis of the epilithic algal community (EAC) of a coral reef were studied. Photosynthesis and respiration of the EAC of dead coral surfaces were investigated for samples from two locations: the Gulf of Aqaba, Eilat (Israel), and One Tree Reef on the Great Barrier Reef (Australia). Microsensors were used to measure O₂ and pH at the EAC surface and above. Oxygen profiles in the light and dark indicated a diffusive boundary layer (DBL) thickness of 180–590 μm under moderate flow (~0.08 m s^?1) and >2,000 μm under quasi-stagnant conditions. Under light saturation the oxygen concentration at the EAC surface rose within a few minutes to 200–550% air saturation levels under moderate flow and to 600–700% under quasi-stagnant conditions. High maximal rates of net photosynthesis of 8–25 mmol O₂ m^?2 h^?1 were calculated from measured O₂ concentration gradients, and dark respiration was 1.3–3.3 mmol O₂ m^?2 h^?1. From light–dark shifts, the maximal rates of gross photosynthesis at the EAC surface were calculated to be 16.5 nmol O₂ cm^?3 s^?1. Irradiance at the onset of saturation of photosynthesis, E _k, was <100 µmol photons m^?2 s^?1, indicating that the EAC is a shade-adapted community. The pH increased from 8.2 in the bulk seawater to 8.9 at the EAC surface, suggesting that very little carbon in the form of CO₂ occurs at the EAC surface. Thus the major source of dissolved inorganic carbon (DIC) must be in the form of HCO₃ ^?. Estimates of DIC fluxes across the DBL indicate that, throughout most of the daytime under in situ conditions, DIC is likely to be a major limiting factor for photosynthesis and therefore also for primary production and growth of the EAC.     

Towards carrying capacity assessment for aquaculture in the Bolinao Bay, Philippines: A numerical study of tidal circulation

The increase in intensive aquaculture production in the Bolinao Bay, Philippines reached the point of harmful influence to production stock. Up to the present, there has been no estimation of aquaculture carrying capacity which is based on quantification of processes responsible for (a) water quality inside the units, (b) impact to the seabed and (c) water quality in the whole Bolinao Bay. The numerical estimation of tidal circulation, which is the most dominant part of the hydrodynamical regime in the Bolinao Bay, represents an unavoidable step in the carrying capacity determination considering points (a), (b) and (c). The hydrodynamical model we apply is a free surface, 3D finite element tidal model, forced with sea elevation dynamics at three open boundaries. It incorporates an increase in bottom stress drag coefficients in the coral areas and advanced transport corrected advection scheme. The model simulations of water flow show good agreement with measured currents in the central part of the Bolinao Bay, southern and northeastern channel, while agreement in the areas in vicinity of northern channel is not as tight. In order to provide an insight into the water exchange in aquaculture units, to support local water quality models and seabed deposition models (points (a) and (b)), the area is mapped with neap tide mean, spring tide mean and 14-day mean current velocity contours. The highest 14-day mean velocities are attained in the southern channel (>17 cm s⁻¹), while the mean velocities characteristic for northern part (<6 cm s⁻¹) and shallow areas of coral reefs (<2 cm s⁻¹) are much lower. Area-mean difference between spring tide and 14-day mean velocities is estimated to be 18.32% (std=7.31%), while difference between neap tide and 14-day mean velocities is 17.62 % (std=11.19%). To support global basin-wide water quality models (point (c)), retention of water in the bay is estimated by Lagrangian and Eulerian calculation procedure of mean residence time field. Both calculations estimate the highest (no-wind) residence times (Lan: 25.4 days, Eul: 21.03 days) in the central northern part, but most of the area (Lan: 73.11%, Eul: 79.31%) is characterized by residence time values significantly lower than 15 days. The results are readily applicable for upcoming implementation in the models of local (aquaculture units, seabed) and basin-wide (Bolinao Bay) nutrient dynamics, primary and secondary production, organic matter decomposition and oxygen dynamics.     

An experimental analysis of lunule function in the sand dollar Mellita quinquiesperforata

Coefficients of lift and drag for fifty lunulate sand dollars [Mellita quinquiesperforata (Leske 1778)], poised over a flat, sandy surface, were determined at seven different air stream velocities. Coefficients of static friction were obtained experimentally on level sand in standing water. Critical velocity, at which a sand dollar would be dislodged, is defined as that current velocity in which the force of drag is equal to the product of residual weight (i.e. weight-lift) and the coefficient of static friction. Small individuals have lower critical velocities than large ones: 26 cm s^?1 and 43 cm s^?1 for individuals 48 and 94 mm in diameter, respectively. Measurements were repeated for 20 of the controls, their lunules smoothly filled with modelling clay, and 20 from which lunule spines were removed. In every case, blocking the lunules decreased critical velocity, by an average of 15%; this was shown to be statistically significant (P>0.01). Removal of spines, which partially occlude the lunules, significantly (P>0.01) increased critical velocity by an average of 13%. Direct measurements in a water tunnel showed that calculated values of critical velocity were slightly too high, but otherwise confirmed the role of the lunules. Allometric analysis showed that as sand dollars increase in size, the distances of the lunules from the center of the disk increase less rapidly than the radii. A principal components' analysis of profile measurements of nine species indicated that lunulate and non-lunulate sand dollars are quite different in form. Lunulate species tend to be thinedged, flat-domed and larger in diameter. Some alternative ideas of lunule function are discussed and it is shown that there are no allometric or experimental data to support any of the suggested feeding hypotheses.     

Characterisation and classification of hoarfrost samples collected in Poland (2003–2005) by discriminant analysis

Discharges of nutrients, urea, dissolved organic matter and heavy metals by a sewage underwater pipeline are analysed in comparison to environmental conditions in a shallow coastal zone. Variable thermo-haline stratifications of the water column and currents in upper (2.62–34.97 cm s^?1) and deeper (0.83–10.91 cm s^?1) layers drive vertical diffusion and lateral transport of wastewaters. Loads of reactive phosphorus (0.13 tons d^?1) and ammonium (1.62 tons d^?1) by the pipeline are not negligible compared to the major river loads in the gulf. High concentrations of urea (≤11.51 μmol N dm^?3) were found in the area of wastewater release. Ammonium uptake (6.14–534 nmol N dm^?3 h^?1) strongly exceeded nitrate uptake (0.19–138 nmol N dm^?3 h^?1), indicating that discharges of ammonium by the pipeline are actively assimilated by plankton community even at low levels of light. Distribution of Zn (≤27.7 ppb), Cu (≤25.6 ppb), Cd (≤0.80 ppb) and Pb (≤13.5 ppb) in the water column and the measurement of their complex-forming capacity in seawater did not indicate a persistent perturbation of the pelagic environment due to heavy metals.     

In situ swimming and settlement behaviour of larvae of an Indo-Pacific coral-reef fish, the coral trout Plectropomus leopardus (Pisces: Serranidae)

Late larvae of the serranid coral trout Plectropomus leopardus (Lacepède), captured in light traps, were released during the day both in open water and adjacent to two reefs, and their behaviour was observed by divers at Lizard Island, northern Great Barrier Reef. Coral trout larvae (n = 110) were present in light-trap catches from 18 November to 3 December 1997, including new moon (30 November). The swimming speed of larvae in open water or when swimming away from reefs was significantly greater (mean 17.9 cm s⁻¹) than the speed of larvae swimming towards or over reefs (mean 7.2 cm s⁻¹). Near reefs, larvae swam at average depths of 2.7 to 4.2 m, avoiding 0 to 2 m. In open water, swimming depth varied with location: larvae >1 km east of Lizard Island swam steeply downward to >20 m in 2 to 4 min; larvae >1 km west oscillated between 2.6 and 13 m; larvae 100 to 200 m east of Lizard Island oscillated between 0.8 and 15 m. Nearly all larvae swam directionally in open water and near reefs. In open water, the average swimming direction of all larvae was towards the island, and 80% (4 of 5) swam directionally (p < 0.05, Rayleigh's test). Larvae swam directionally over the reef while looking for settlement sites. The frequency of behaviours by larvae differed between two reefs of different exposure and morphology. Depending on site, 26 to 32% of larvae released adjacent to reefs swam to open water: of these, some initially swam towards or over the reef before swimming offshore. In some cases, offshore-swimming seemed to be due to the presence of predators, but usually no obvious cause was observed. Depending on the reef, 49 to 64% of the larvae settled. Non-predatory reef residents aggressively approached 19% of settlers. Between 5 and 17% of the larvae were eaten while approaching the reef or attempting to settle, primarily by lizardfishes but also by wrasses, groupers and snappers. A higher percentage of larvae settled in the second week of our study than in the first. Average time to settlement was short (138 s ± 33 SE), but some larvae took up to 15 min to settle. Average settlement depth was 7.5 to 9.9 m, and differed between locations. No settlement took place on reef flats or at depths <4.2 m. Larvae did not appear to be selective about settlement substrate, but settled most frequently on live and dead hard coral. Late-stage larvae of coral trout are capable swimmers with considerable control over speed, depth and direction. Habitat selection, avoidance of predators and settlement seem to rely on vision. Received: 7 July 1998 / Accepted: 26 January 1999     

水动力条件对藻类影响的研究进展

水动力过程是影响水体富营养化状态和水华爆发的重要因素,水动力因素对藻类影响的研究对于富营养化水体藻类控制具有重要意义。归纳分析近年来关于流速、流态对藻类生长和种类变化的研究报道;就水动力条件对藻类的影响及其作用机理等详细地进行了文献综述。水动力条件对藻类生长的影响分为流速和流态两个方面,不论是单一藻种还是混合藻类,低流速、小扰动有利于藻类的生长和聚集,流速增大则导致Chla浓度先递增后递减,不同藻类的临界流速并不相同;藻类生长随着湍流程度的增加而逐渐受到抑制,抑制作用与水流流态（层流、过渡流、湍流）无明显相关关系,水体流态的变化造成水流剪应力的变化,藻类种类的差异导致其对水流剪应力的响应变化。水动力条件变化引起的藻类种群结构变化,主要表现为水体混合加剧导致优势种群的转换。水动力条件对藻类影响的作用原理主要是引起了光强的改变、细胞长度的变化、营养盐运送及捕食行为变化等。综观当前的研究成果,水动力能否真正阻止藻类细胞的生长或聚集,影响藻类生长或种类变化的扰动的最低水平以及水动力对藻类影响的作用机理是这一领域未来研究的重点所在。     

Environmental impact of CO<Subscript>2</Subscript>, Rn,Hg degassing from the rupture zones produced by Wenchuan <Emphasis Type="Italic">M</Emphasis><Subscript>s</Subscript> 8.0 earthquake in western Sichuan,China

The concentrations and flux of CO₂, ²²²Radon (Rn), and gaseous elemental mercury (Hg) in soil gas were investigated based on the field measurements in June 2010 at ten sites along the seismic rupture zones produced by the May 12, 2008, Wenchuan M _s 8.0 earthquake in order to assess the environmental impact of degassing of CO₂, Rn and Hg. Soil gas concentrations of 344 sampling points were obtained. Seventy measurements of CO₂, Rn and Hg flux by the static accumulation chamber method were performed. The results of risk assessment of CO₂, Rn and Hg concentration in soil gas showed that (1) the concentration of CO₂ in the epicenter of Wenchuan M _s 8.0 earthquake and north end of seismic ruptures had low risk of asphyxia; (2) the concentrations of Rn in the north segment of seismic ruptures had high levels of radon, Maximum was up to level 4, according to Chinese code (GB 50325-2001); (3) the average geoaccumulation index I _geo of soil Hg denoted the lack of soil contamination, and maximum values classified the soil gas as moderately to strongly polluted in the epicenter. The investigation of soil gas CO₂, Rn and Hg degassing rate indicated that (1) the CO₂ in soil gas was characterized by a mean \(\updelta^{13}C_{CO2}\) of ?20.4 ‰ and by a mean CO₂ flux of 88.1 g m^?2 day^?1, which were in the range of the typical values for biologic CO₂ degassing. The maximum of soil CO₂ flux reached values of 399 g m^?2 day^?1 in the epicenter; (2) the soil Rn had higher exhalation in the north segment of seismic ruptures, the maximum reached value of 1976 m Bq m^?2 s^?1; (3) the soil Hg flux was lower, ranging from ?2.5 to 18.7 n g m^?2 h^?1 and increased from south to north. The mean flux over the all profiles was 4.2 n g m^?2 h^?1. The total output of CO₂ and Hg degassing estimated along seismic ruptures for a survey area of 18.17 km² were approximately 0.57 Mt year^?1 and 688.19 g year^?1. It is recommended that land-use planners should incorporate soil gas and/or gas flux measurements in the environmental assessment of areas of possible risk. A survey of all houses along seismic ruptures is advised as structural measures to prevent the ingress of soil gases, including CO₂ and Rn, were needed in some houses.     

Change in zooxanthellae and mucocyte tissue density as an adaptive response to environmental stress by the coral, <Emphasis Type="Italic">Montastraea annularis</Emphasis>

Results from controlled in situ experimentation conducted on the leeward reef tract of Curaçao, Netherlands Antilles, indicate that the coral Montastraea annularis exhibits a complex, yet consistent, cellular response to increasing sea surface temperature (SST) and decreasing irradiance. This was determined by simultaneously quantifying and tracking the tissue density of zooxanthellae and mucocytes using a novel technique that integrates the lectin histochemical stain wheat germ agglutinin (WGA) with high-resolution (200 nm) optical epifluorescence microscopy. Coral colonies growing at 6-m water depth (WD) and an irradiance of 100.2 ± 6.5 μmol m^?2 s^?1 were treated with a shading experiment for 11 days that reduced irradiance to 34.9 ± 6.6, 72.0 ± 7.0 and 90.1 ± 4.2 μmol m^?2 s^?1, respectively. While a significant decrease in the density of both zooxanthellae and mucocytes were observed at all shade levels, the largest reduction occurred between the natural non-shaded control (44,298 ± 3,242 zooxanthellae cm^?2; 4,853 ± 346 mucocytes cm^?2) and the highest shading level (13,982 ± 1,961 zooxanthallae cm^?2; 2,544 ± 372.9 mucocytes cm^?2). Colonies were also sampled during a seasonal increase in SST of 1.5°C, where the density of zooxanthellae was significantly lower (from 54,710 ± 1,755 to 34,322 ± 2,894 cells cm^?2) and the density of mucocytes was significantly higher (from 6,100 ± 304 to 29,658 ± 3,937 cells cm^?2). These observations of coral cellular response to environmental change provide evidence to support new hypotheses for coral survival and the complex role played by mucus in feeding, microbial associations and resilience to increasing SST.     

Estimating river flow effects on water ages by hydrodynamic modeling in Little Manatee River estuary, Florida, USA

The water age in a tidal river in Florida, Little Manatee River, has been investigated in this study by the application of a three-dimensional hydrodynamic model. In response to a pulse dye release in the upper end of the river boundary, the hydrodynamic model determines the water age for a given location by recording the time for the dye to reach the given river location. The hydrodynamic model uses horizontal curvilinear orthogonal grids to represent the complex river system that includes several bayous and tributaries. The model has was calibrated and verified in previous study by using two continuous data sets for a 6 month period. Satisfactory model verifications indicate that the hydrodynamic model is capable of quantifying the mixing and transport process for calculating the water age in the tidal river. For 17 freshwater inflow scenarios in the Little Manatee River, the hydrodynamic model was applied to simulate water ages along the main channel of the river at 2-km interval. Flow rates in the 17 scenarios varying from 0.26 to 76.56 m³/s cover the range of the observed flows in the Little Manatee River. Water ages from model predictions range from the minimum 1.2 days under the maximum 76.56 m³/s inflow condition to the 50 days under the minimum 0.26 m³/s inflow condition. Empirical regression equations at three selected stations, with the correlation coefficient R² above 0.96, were derived from numerical model simulations to correlate water ages to freshwater inflows. The empirical water-age equation derived from hydrodynamic model simulations can be used to provide quick and low-cost estimations of water ages in response to various inflow scenarios for studying physical–chemical and biological processes in the river.     

Differences in kelp morphology between wave sheltered and exposed localities: morphologically plastic or fixed traits?

The ability of algae to change the shape of their thallus in response to the environment may be of functional and ecological importance to the alga, with many species of macroalgae exhibiting a great range of morphological variation across wave exposure gradients. However, differences in morphology detected between sheltered and exposed environments cannot determine whether such differences represent plastic responses to the local environment or whether morphology is genetically fixed. This study tested for differences in the morphology of the common kelp, Ecklonia radiata, between wave sheltered and exposed environments, and reciprocally transplanted juveniles to distinguish the nature of such differences (i.e. plastic vs fixed traits). Differences between exposure environments were consistent with known effects of exposure (i.e. a wide, thin thallus at sheltered sites and a narrow, thick thallus with a thick stipe at exposed sites). The reciprocal transplant experiment confirmed that morphological plasticity was the mechanism enabling this alga to display different patterns in morphology between exposure environments. Individuals transplanted to the exposed environment underwent a rapid and extreme response in morphology, which was not apparent in individuals transplanted to the sheltered environment that responded more slowly. These results suggest that stressors typical of sheltered environments (i.e. diffusion stress) may not be as influential (if at all) compared to stressors typical of exposed environments (i.e. breakage, dislodgement) in differentiating morphological characters between exposure environments.     

Spatial distributions,source apportionment and ecological risk of SVOCs in water and sediment from Xijiang River,Pearl River Delta

Xijiang River is an important drinking water source in Guangxi Province, China. Along the Xijiang River and surrounding tributary, the pollution profile of three important groups of semi-volatile organic compounds, including polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs) and phthalate esters (PAEs), was analyzed. Relatively low levels of PAHs (64–3.7 × 10² ng L^?1) and OCPs (16–70 ng L^?1), but high levels of PAEs (7.9 × 10²–6.8 × 10³ ng L^?1) occurred in the water. Comparatively, low levels of OCPs (39–1.8 × 10² ng g^?1) and PAEs (21–81 ng g^?1), but high levels of PAHs (41–1.1 × 10³ ng g^?1) were found in sediment. Principal component analyses for source identification indicated petroleum-derived residues or coal and biomass combustion, and vehicular emission was the main sources for PAHs. The OCPs sources of each category were almost independent, whereas the new input of HCHs and p,p′-DDTs probably existed in some areas. PAEs were mainly originated from personal care products of urban sewage, plastic and other industrial sources. Ecological risk through the risk quotient analysis indicated a small or significant potential adverse effect on fish, daphnia and green algae. Nevertheless, the integrated risk of all pollutants should be taken into account in future study.     

Fast and complete removal of the 5-fluorouracil drug from water by electro-Fenton oxidation

Cytostatic drugs are a troublesome class of emerging pollutants in water owing to their potential effects on DNA. Here we studied the removal of 5-fluorouracil from water using the electro-Fenton process. Galvanostatic electrolyses were performed with an undivided laboratory-scale cell equipped with a boron-doped diamond anode and a carbon felt cathode. Results show that the fastest degradation and almost complete mineralization was obtained at a Fe²⁺ catalyst concentration of 0.2 mM. The absolute rate constant for oxidation of 5-fluorouracil by hydroxyl radicals was 1.52 × 10⁹ M^?1 s^?1. Oxalic and acetic acids were initially formed as main short-chain aliphatic by-products, then were completely degraded. After 6 h the final solution mainly contained inorganic ions (NH₄ ⁺, NO₃ ^? and F^?) and less than 10% of residual organic carbon. Hence, electro-Fenton constitutes an interesting alternative to degrade biorefractory drugs.     

Deep-sea caging of the mussel Mytilus galloprovincialis: Potential application in ecotoxicological studies

We experimented with caging the Mediterranean mussel (Mytilus galloprovincialis) at various depths for 69 d to measure basic physiological parameters, histological response and bio-accumulation of contaminants in a deep-sea contaminated area. In preliminary experiments, we demonstrated, under artificial pressure conditions, the ability of mussels Mytilus galloprovincialis to tolerate rapid immersion (at a speed of up to 120 m min^?1). In situ experiments were performed using submerged lines enabling mussels to be maintained at depths ranging of 40–1550 m with survival rates ranging from 80 to 38%, respectively. No significant differences in condition indexes were observed between treated and control specimens. However, histological observations demonstrated a clear reduction in thickness of the digestive epithelium with increasing depth exposure. By determining the contaminants in caged mussels, we found the following values for chromium accumulation: 27.4 μg g^?1 dry weight at 580 m depth and 9.8 μg g^?1 dry weight at 1550 m. Selected stations were located downstream of an industrial effluent at 420 m. The biological and environmental consequences of deep-sea contamination demonstrate the suitability of caged mussels for monitoring contaminant accumulation.     

Development and testing of a micro wind tunnel for on-site wind erosion simulations

Wind erosion processes affect soil surfaces across all land uses worldwide. Understanding the spatial and temporal scales of wind erosion is a challenging undertaking because these processes are diverse and highly variable. Wind tunnels provide a useful tool as they can be used to simulate erosion at small spatial scales. Portable wind tunnels are particularly valued because erosion can be simulated on undisturbed soil surfaces in the field. There has been a long history of use of large portable wind tunnels, with consensus that these wind erosion simulation tools can meet real world aerodynamic criteria. However, one consequence of striving to meet aerodynamic reality is that the size of the tunnels has increased, making them logistically difficult to work with in the field and resulting in a tendency to homogenise naturally complex soil surfaces. This homogenisation is at odds with an increasing awareness of the importance that small scale processes have in wind erosion. To address these logistical and surface homogenisation issues we present here the development and testing of a micro wind tunnel (MWT) designed to simulate wind erosion processes at high spatial resolution. The MWT is a duct-type design—0.05 m tall 0.1 m wide and with a 1.0 m working section. The tunnel uses a centrifugal motor to suck air through a flow‐conditioning section, over the working section and then through a sediment collection trap. Simulated wind velocities range from 5 to 18 m s^?1, with high reproducibility. Wind speeds are laterally uniform and values of u _* at the tunnel bed (calculated by measuring the pressure gradients within the MWT) are comparable with those of larger tunnels in which logarithmic profiles can be developed. Saltation sediment can be added. The tunnel can be deployed by a single person and operated on slopes ranging from 0 to 10°. Evidence is presented here that the MWT provides new and useful understanding of the erodibility of rangelands, claypans and ore stockpiles.     

Transport of Sewage Sludge in a Mixed Water Column

Abstract

Sewage sludge from four publicly-owned treatment works was sampled and characterized in terms of parameters affecting transport at the 106-mile deep ocean disposal site as part of the US Environmental Protection Agency's site monitoring programme. Samples from treatment plants in Passaic Valley, Rahway, and Elizabeth, New Jersey and New York City were characterized in terms of dynamic size distribution, suspended solids and density. the transport characteristics of sludge particles were measured using a 2 metre computer-interfaced laboratory settling column. Experiments were conducted at constant salinity (35 ppt) while varying hydrodynamic mixing, sludge type and concentration using a modified factorial experimental design. Hydrodynamic power dissipation was varied so that the vertical dispersion and rms fluid shear rate ranged between 0-6 cm²S^?1 and 0-30s^?1 respectively. Results indicate that at least 80% of suspended sludge particles will eventually settle under mixed conditions. the average settling velocities ranged between 0.05-4.05 × 10^-3 cm ^s-1. Shear rates above 15 s^?1 inhibited sludge settling due to aggregate breakup and boundary effects, but at a lower shear rate, differential settling and fluid shear were the dominant transport mechanisms. Sludge dilution (1/500-1/5000) had a limited effect on the settling rate. Results from this study can be used to calibrate particle transport models to determine the fate of sludge disposed at an ocean disposal site.     

Interactive effects of UV radiation and enhanced temperature on photosynthesis, phlorotannin induction and antioxidant activities of two sub-Antarctic brown algae

Lessonia nigrescens and Durvillaea antarctica, two large sub-Antarctic brown algae from the southern Chilean coast, were exposed to solar UV radiation in an outdoor system during a summer day (for 11 h) as well as to artificial UV radiation under controlled laboratory conditions at two temperatures (15 and 20 °C) for 72 h. Chlorophyll a fluorescence–based photoinhibition of photosynthesis was measured during the outdoor exposure, while electron transport rates, lipid peroxidation, antioxidant activity and content of phlorotannins were determined at different time intervals during the laboratory exposure. Under natural solar irradiances in summer, both species displayed well-developed dynamic photoinhibition: F _v/F _m values decreased by 70 % at noon coinciding with the levels of PAR >1,500 μmol m^?2 s^?1 and UV-B radiation >1 W m^?2 and recovered substantially in the afternoon. In treatments including UV radiation, recovery in D. antarctica started already during the highest irradiances at noon. The results from laboratory exposures revealed that (a) elevated temperature of 20 °C exacerbated the detrimental effects of UV radiation on photochemical parameters (F _v/F _m and ETR); (b) peroxidative damage measured as MDA formation occurred rapidly and was strongly correlated with the decrease in F _v/F _m, especially at elevated temperature of 20 °C; (c) the antioxidant activity and increases in soluble phlorotannins were positively correlated mainly in response to UV radiation; (d) phlorotannins were rapidly induced but strongly impaired at 20 °C. In general, short-term (2–6 h) exposures to enhanced UV radiation and temperature were effective to activate the photochemical and biochemical defenses against oxidative stress, and they continued operative during 72 h, a time span clearly exceeding the tidal or diurnal period. Furthermore, when algae were exposed to dim light and control temperature of 15 °C for 6 h, F _v/F _m increased and lipid peroxidation decreased, indicating consistently that algae retained their ability for recovery. D. antarctica was the most sensitive species to elevated temperature for prolonged periods in the laboratory. Although no conclusive evidence for the effect of the buoyancy of fronds was found, the interspecific discrepancies in thermo-sensitivity in the UV responses found in this study are consistent with various ecological and biogeographical differences described for these species.