Controlling the fate of roxarsone and inorganic arsenic in poultry litter

A growing body of literature reports 3-nitro-4-hydroxyphenylarsonic acid (roxarsone) degradation in poultry litter (PL) to the more toxic inorganic arsenic (As). Aluminum-based drinking-water treatment residuals (WTR) present a low-cost amendment technology to reduce As availability in PL, similar to the use of alum to reduce phosphorus availability. Batch experiments investigated the effectiveness of WTR in removing roxarsone and inorganic As species from PL aqueous suspensions. Incubation experiments with WTR-amended PL evaluated the effects of WTR application rates (2.5-15% by weight) and incubation time (up to 32 d) at two incubation temperatures (23 and 35 degrees C) on As availability in PL. Batch PL aqueous experiments showed the high affinity of As(V), As(III), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA), and roxarsone for the WTR. The 10% WTR amendment rate decreased As availability in PL by half of that of the unamended (no WTR) PL-incubated samples. The reduction in dissolved As concentrations during incubation of WTR-amended PL samples was kinetically limited, being complete within 13 d. Parallel reductions in roxarsone, As(V), and DMA concentrations were observed with liquid chromatography-inductively coupled plasma mass spectrometry, whereas As(III) and MMA concentrations were always <5% of dissolved As. Incubation temperature did not significantly (p > 0.05) influence dissolved As concentrations in the WTR-amended PL. Potential formation of a copper-containing roxarsone metabolite was considered in PL aqueous suspensions with the aid of electrospray mass spectrometry. Further experiments in the field are necessary to ensure that sorbed As is stable in WTR-amended PL.     

Anaerobic treatment with methane recovery of agricultural and village wastes

To provide more efficient utilization of village wastes and agricultural residues and eliminate pollution from current practices anaerobic treatment of such wastes with methane recovery is proposed. This paper describes studies to determine the performance of anaerobic composting for such wastes.Composting of three waste types was investigated: (a) agricultural residues, (b) Village solid waste and 1:1 mixture of (a) and (b). Three 150 gallon reactors with periodic leachate recycling were used for anaerobic composting while three 50 gallon reactors produced an aerobic compost from the same wastes. Reactors were maintained for six months simulating the time between growing seasons. Volatile solids destruction ranged from 10.3 to 38.7%. Biogas production was ranged from 1350 to 1420 1 per k of volatile solids destroyed with a methane content of 55 to 70%. Leachate was monitored throughout for: pH, alkalinity, total and ammonia nitrogen, COD, TOC, total solids, volatile solids and four metals. MPN data was collected for various bacterial groups (total plate count, anaerobic cellulose decomposers, and anaerobic acid producers) to monitor increases and declines in the leachate bacterial population. Physical and chemical testing provided comparisons between finished anaerobic compost and aerobic compost from the same three waste samples.     

Trace element speciation in poultry litter

Trace elements are added to poultry feed for disease prevention and enhanced feed efficiency. High concentrations are found in poultry litter (PL), which raises concerns regarding trace element loading of soils. Trace metal cation solubility from PL may be enhanced by complexation with dissolved organic carbon (DOC). Mineralization of organo-As compounds may result in more toxic species such as As(III) and As(V). Speciation of these elements in PL leachates should assist in predicting their fate in soil. Elemental concentrations of 40 PL samples from the southeastern USA were determined. Water-soluble extractions (WSE) were fractionated into hydrophobic, anionic, and cationic species with solid-phase extraction columns. Arsenic speciation of seven As species, including the main As poultry feed additives, roxarsone (ROX; 3-nitro-4-hydroxyphenylarsonic acid) and p-arsanilic acid (p-ASA; 4-aminophenylarsonic acid), was performed by ion chromatography-inductively coupled plasma-mass spectrometry (IC-ICP-MS). Total As concentrations in the litter varied from 1 to 39 mg kg(-1), averaging 16 mg kg(-1). Mean total Cu, Ni, and Zn concentrations were 479, 11, and 373 mg kg(-1), respectively. Copper and Ni were relatively soluble (49 and 41% respectively) while only 6% of Zn was soluble. Arsenic was highly soluble with an average of 71% WSE. Roxarsone was the major As species in 50% of PL samples. However, the presence of As(V) as the major species in 50% of the PL samples indicates that mineralization of ROX had occurred. The high solubility of As from litter and its apparent ready mineralization to inorganic forms coupled with the large quantity of litter that is annually land-applied in the USA suggests a potential detrimental effect on soil and water quality in the long term.     

Nitrification and denitrification gene abundances in swine wastewater anaerobic lagoons

Although anaerobic lagoons are used globally for livestock waste treatment, their detailed microbial cycling ofN is only beginning to become understood. Within this cycling, nitrification can be performed by organisms that produce the enzyme ammonia monooxygenase. For denitrification, the reduction of nitrite to nitric oxide can be catalyzed by two forms of nitrite reductases, and N,O can be reduced by nitrous oxide reductase encoded by the gene nosZ The objectives of this investigation were to (i) quantify the abundance of the amoA, nirK, nirS, and nosZ genes; (ii) evaluate the influence of environmental conditions on their abundances; and (iii) evaluate their abundance relative to denitrification enzyme activity (DEA). Samples were analyzed via real-time quantitative polymerase chain reaction and collected from eight typical, commercial anaerobic, swine wastewater lagoons located in the Carolinas. The four genes assayed in this study were present in all eight lagoons. Their abundances relative to total bacterial populations were 0.04% (amoA), 1.33% (nirS), 5.29% (nirK), and 0.27% (nosZ). When compared with lagoon chemical characteristics, amoA and nirK correlated with several measured variables. Neither nirS nor nosZ correlated with any measured environmental variables. Although no gene measured in this study correlated with actual or potential DEA, nosZ copy numbers did correlate with the disparity between actual and potential DEA. Phylogenetic analysis ofnosZdid not reveal any correlations to DEA rates. As with other investigations, analyses of these genes provide useful insight while revealing the underlying greater complexity of N cycling within swine waste lagoons.     

Transformation of diphenylarsinic acid in agricultural soils

We investigated the transformation and fate of diphenylarsinic acid (DPAA) during incubation in two types of soils (Entisol and Andisol) under aerobic and anaerobic conditions. Under anaerobic conditions only, DPAA was transformed into methyldiphenylarsine oxide by methylation. Under both aerobic and anaerobic conditions, DPAA was degraded to phenylarsonic acid by dephenylation, and phenylarsonic acid was subsequently methylated to form methylphenylarsinic acid and dimethylphenylarsine oxide. The degradation of DPAA in the Andisol was less extensive than in the Entisol. In autoclaved soil under anaerobic conditions, DPAA underwent little degradation during the 24-wk incubation. In unautoclaved soils, the concentration of DPAA in soil clearly decreased after 24 wk of incubation, indicating that DPAA degradation was driven by microbial activity.     

Reduction of malodorous compounds from a treated swine anaerobic lagoon

There is a need for treatment technologies that can eliminate environmental problems associated with anaerobic lagoons. These technologies must be able to capture nutrients, kill pathogens, and reduce emissions of ammonia and nuisance odors. To meet these needs, a full-scale wastewater treatment plant was installed as a demonstration project on one of three 4360-pig (Sus scrofa) production units in a finishing farm in Duplin County, North Carolina. Once the treatment plant was operational, flow of raw manure into the unit's corresponding lagoon was discontinued and the lagoon was used to store treated wastewater. Water quality was monitored in the converted lagoon and in the two conventional lagoons. A gas chromatographic method was developed to measure concentration of five selected malodorous compounds (phenol, p-cresol, 4-ethylphenol, indole, and skatole) in liquid lagoon samples. Dramatic improvements in the water quality parameters TKN, NH3-N, solids, COD, and BOD in the converted waste lagoon paralleled reductions in malodorous compounds. Nine months after conversion, identified malodorous compounds in liquid extractions averaged 6.6 and 38.8 ng mL(-1) in water from the converted lagoon and the conventional lagoons, respectively. The reduction was particularly marked for p-cresol, 4-ethylphenol, and skatole, all of which make important contributions to swine waste odors due to their characteristic odors and low detection thresholds.     

Improvement of fruit and vegetable waste anaerobic digestion performance and stability with co-substrates addition

The effect of fish waste (FW), abattoir wastewater (AW) and waste activated sludge (WAS) addition as co-substrates on the fruit and vegetable waste (FVW) anaerobic digestion performance was investigated under mesophilic conditions using four anaerobic sequencing batch reactors (ASBR) with the aim of finding the better co-substrate for the enhanced performance of co-digestion. The reactors were operated at an organic loading rate of 2.46–2.51 g volatile solids (VS) l⁻¹ d⁻¹, of which approximately 90% were from FVW, and a hydraulic retention time of 10 days. It was observed that AW and WAS additions with a ratio of 10% VS enhanced biogas yield by 51.5% and 43.8% and total volatile solids removal by 10% and 11.7%, respectively. However FW addition led to improvement of the process stability, as indicated by the low VFAs/Alkalinity ratio of 0.28, and permitted anaerobic digestion of FVW without chemical alkali addition. Despite a considerable decrease in the C/N ratio from 34.2 to 27.6, the addition of FW slightly improved the gas production yield (8.1%) compared to anaerobic digestion of FVW alone. A C/N ratio between 22 and 25 seemed to be better for anaerobic co-digestion of FVW with its co-substrates. The most significant factor for enhanced FVW digestion performance was the improved organic nitrogen content provided by the additional wastes. Consequently, the occurrence of an imbalance between the different groups of anaerobic bacteria which may take place in unstable anaerobic digestion of FVW could be prevented.     

Evaluation of Biogas Yield of Selected Ratios of Cattle,Swine, and Poultry Wastes

Production of biogas from animal wastes could lessen the problems of energy shortage and indiscriminate animal waste disposal. A study of anaerobic digestion of selected ratios of cattle, swine, and poultry wastes was carried out to evaluate their biogas yields. Cattle (C), swine (S), and poultry (P) wastes were mixed as C:S:P in the following ratios: 1:0:0 (control), 1:0:1, 4:1:3, 2:1:1, 4:3:1, and 1:1:0 by mass to obtain six samples of of 0.4 kg each, referred to as samples 1 to 6 respectively. A quantity (0.1 kg) of inoculum (obtained by pre-fermenting equal masses of poultry waste and water for 50 days under anaerobic condition) and 0.5 kg of water were added to each of the samples. The resulting slurries were digested in triplicates for 30 days in 1.3 L laboratory-scale anaerobic digesters. The volume of biogas produced was obtained by downward displacement of water in a measuring cylinder. The cumulative biogas yields of samples 1 to 6 were 332.5, 497.5, 487.5, 467.5, 457.5, and 430.0 cm³/kg slurry respectively. The cumulative biogas yields of samples 2 and 3 were significantly (p < 0.05) higher than those of the other samples but not significantly (p > 0.05) different from each other. However, the cumulative biogas yield of sample 1 was significantly (p < 0.05) lower than those of the other samples. The study revealed that a blend of equal masses of cattle and poultry wastes is optimum for biogas production.     

Tracing nitrate transport and environmental impact from intensive swine farming using delta nitrogen-15

Natural-abundance delta15N showed that nitrate generated from commercial land application of swine (Sus scrofa domesticus) waste within a North Carolina Coastal Plain catchment was being discharged to surface waters by ground water passing beneath the sprayfields and adjacent riparian buffers. This was significant because intensive swine farms in North Carolina are considered non-discharge operations, and riparian buffers with minimum widths of 7.6 m (25 ft) are the primary regulatory control on ground water export of nitrate from these operations. This study shows that such buffers are not always adequate to prevent discharge of concentrated nitrate in ground water from commercial swine farms in the Mid-Atlantic Coastal Plain, and that additional measures are required to ensure non-discharge conditions. The median delta15N-total N of liquids in site swine waste lagoons was +15.4 +/- 0.2% vs. atmospheric nitrogen. The median delta15N-NO3 values of shallow ground water beneath and adjacent to site sprayfields, a stream draining sprayfields, and waters up to 1.5 km downstream were + 15.3 +/- 0.2 to + 15.4 +/- 0.2%. Seasonal and spatial isotopic variations in lagoons and well waters were greatly homogenized during ground water transport and discharge to streams. Neither denitrification nor losses of ammonia during spraying significantly altered the bulk ground water delta15N signal being delivered to streams. The lagoons were sources of chloride and potassium enrichment, and shallow ground water showed strong correlation between nitrate N, potassium, and chloride. The 15N-enriched nitrate in ground water beneath swine waste sprayfields can thus be successfully traced during transport and discharge into nearby surface waters.     

POTENTIAL IMPACT OF EARTHEN WASTE STORAGE STRUCTURES ON WATER RESOURCES IN IOWA1

ABSTRACT: Earthen waste storage structures (EWSS) associated with large confined (concentrated) animal feeding operations (CAFOs) were evaluated for their potential to impact water resources in Iowa. A representative sample of 34 EWSS from a digital database of 439 lagoons and basins permitted between 1987 and 1994 was analyzed. Eighteen percent (6 of 34) directly overlie alluvial aquifers that are used widely for potable water supply. Ninety‐four percent (29 of 31) were constructed below the water table based on EWSS depth data. At 65 percent of EWSS (22 of 34), 50 percent or more of the manure‐spreading area (MSA) has a water‐table depth less than 1.6 m. At 74 percent of EWSS (25 of 34), 90 percent or more of the MSA contains soil with vertical K exceeding 25.4 mm/hr. Seventy‐one percent (24 of 34) occur where 10 percent or less of the MSA is frequently flooded. No significant differences were found among leakage rates due to aquifer vulnerability class or surficial material. However, at least 50 percent of EWSS (14 of 28) leaked at rates significantly greater than 1.6 mm/d under the new construction standard. The estimated 5,000 unregulated CAFOs may have a greater potential to impact water resources in Iowa.     

Piggery waste treatment by anaerobic digestion and nutrient removal by ionic exchange

Piggery wastes must be treated before their disposal. The high solids content and high chemical oxygen demand of piggery wastes indicated that anaerobic biological treatment could be successfully applied as primary treatment. For that reason, a comparison between upflow anaerobic sludge bed reactor (UASB) and anaerobic fixed bed reactor (AFBR) at a similar organic volumetric loading rate of 5 kg DQO/m³ day was carried out. 60% of the piggery waste COD was removed with the AFBR compared to 40% with the UASB, thus showing a better performance of the AFBR. After 1-h sedimentation secondary process, both anaerobic effluents were treated by ionic exchange with natural zeolite due to their high values of ammoniacal nitrogen (NH⁺₄ plus free NH₃). The high removal of nutrients reported (90%) shows zeolite to be a good choice as tertiary treatment.     

Potential mineralization of four herbicides in a ground water--fed wetland area

Herbicides may leach from agricultural fields into ground water feeding adjacent wetlands. However, only little is known of the fate of herbicides in wetland areas. The purpose of the study was to examine the potential of a riparian fen to mineralize herbides that could leach from an adjacent catchment area. Slurries were prepared from sediment and ground water collected from different parts of a wetland representing different redox conditions. The slurries were amended with O2, NO3-, SO4(2-), and CO2, or CO2 alone as electron acceptors to simulate the in situ conditions and their ability to mineralize the herbides mecoprop, metsulfuron-methyl, isoproturon and atrazine. In addition, the abundance of bacteria able to utilize O2, NO3-, SO4(2-) + CO2, and CO2 as electron acceptors was investigated along with the O2-reducing and methanogenic potential of the sediment. The recalcitrance to bacterial degradation depended on both the type of herbicide and the redox conditions pertaining. Mecoprop was the most readily degraded herbicide, with 36% of [ring-U-14C]mecoprop being mineralized to 14CO2 under aerobic conditions after 473 d. In comparison, approximately 29% of [phenyl-U-14C]metsulfuron-methyl and 16% of [ring-U-14C]isoproturon mineralized in aerobic slurries during the same period. Surprisingly, 8 to 13% of mecoprop also mineralized under anaerobic conditions. Neither metsulfuron-methyl nor isoproturon were mineralized under anaerobic conditions and atrazine was not mineralized under any of the redox conditions examined. The present study is the first to report mineralization of meco-prop in ground water in a wetland area, and the first to report mineralization of a phenoxyalcanoic acid herbicide under both aerobic and anaerobic conditions.     

Effects of ionic strength, temperature, and pH on degradation of selected antibiotics

Aqueous degradation rates, which include hydrolysis and epimerization, for chlortetracycline (CTC), oxytetracycline (OTC), tetracycline (TET), lincomycin (LNC), sulfachlorpyridazine (SCP), sulfadimethoxine (SDM), sulfathiazole (STZ), trimethoprim (TRM), and tylosin A (TYL) were studied as a function of ionic strength (0.0015, 0.050, or 0.084 mg/L as Na(2)HPO(4)), temperature (7, 22, and 35 degrees C), and pH (2, 5, 7, 9, and 11). Multiple linear regression revealed that ionic strength did not significantly affect (alpha = 0.05) degradation rates for all compounds, but temperature and pH affected rates for CTC, OTC, and TET significantly (alpha = 0.05). Degradation also was observed for TYL at pH 2 and 11. No significant degradation was observed for LNC, SCP, SDM, STZ, TRM, and TYL (pH 5, 7, and 9) under study conditions. Pseudo first-order rate constants, half-lives, and Arrhenius coefficients were calculated where appropriate. In general, hydrolysis rates for CTC, OTC, and TET increased as pH and temperature increased following Arrhenius relationships. Known degradation products were used to confirm that degradation had occurred, but these products were not quantified. Half-lives ranged from less than 6 h up to 9.7 wk for the tetracyclines and for TYL (pH 2 and 11), but no degradation of LIN, the sulfonamides, or TRM was observed during the study period. These results indicate that tetracyclines and TYL at pH 2 and 11 are prone to pH-mediated transformation and hydrolysis in some cases, but not the sulfonamides, LIN nor TRM are inclined to degrade under study conditions. This indicates that with the exception of CTC, OTC, and TET, pH-mediated reactions such as hydrolysis and epimerization are not likely removal mechanisms in surface water, anaerobic swine lagoons, wastewater, and ground water.     

Biogas production from slaughterhouse wastewaters and use of biofertilizer obtained for biodegradation of aromatic hydrocarbons

The anaerobic digestion of industrial wastes produces a biogas that is an alternative to the use of fossil fuels for energy production. At the end of this process, the stabilized biomass presents high levels of nutrients, which can be used both as biofertilizers in agriculture and for the biodegradation of contaminants in the soil through improvement of the microbiota. Thus, this study aimed to evaluate biogas production by industrial wastes and to use the biofertilizer for the bioremediation of soils previously contaminated with gasoline. The biomass (420 mL) generated approximately 10 liters (L) of methane and 3 L of other gases. Anaerobic incubation reduced total and volatile solids, as well as biochemical oxygen demand, chemical oxygen demand, and the carbon and nitrogen contents of the biomass. The bioremediation experiment showed that 15 days after contamination with gasoline, the addition of the biofertilizer improved the degradation efficiency of monoaromatic hydrocarbons; however, the degradation of polyaromatic hydrocarbons was less time efficient. So, we conclude that the anaerobic incubation of industrial wastes generates a high amount of biogas, and that biofertilizer deposition into contaminated soil does not affect the efficiency of the degradation of aromatic hydrocarbons after 30 days. Novelty or significance : Anaerobic incubation of industrial wastes generates a high calorific value gas, which can be used as an alternative source of energy. And, the resulting biomass, called biofertilizer, can be used to remediate soils contaminated with hydrocarbons.     

石油工业废弃物处置及其生物治理趋势

鉴于我国石油工作者对石油工业废弃物的普遍关注，现在介绍生物治理的基本概念及ＳＰＥ石油工程师协会中有关含油污泥生物处置的途径及其突出优越性。生物液／固处理工艺（ＬＳＴ）是目前含油污泥处置现场最经济可行的处置方案。这种好氧的ＬＳＴ工艺过程，能够比较充分地代谢和降解石油污泥中的油和脂并大量除去多环芳烃类（ＰＡＨ）化合物及其它有机化合物，明显地降低了石油废弃物对人、畜的毒害程度。其工艺较简单，操作也简易，凡经此工艺处理后的废弃物不留残毒和后患，因此早已被世界上先进国家的炼油业视为处理含油污泥的有效选择了。石油工业废弃物的生物防治和生物降解工艺较大程度地领先于目前我国现场正在执行的各种处理含油废弃物的处理工艺。     

Bioconversion of marine crustacean shell waste

This paper appraises the potential of marine crustacean shell waste from the world's fishing industry for bioconversion to industrial products and envisages almost total low-waste utilisation of such materials. Techniques for the processing of waste shells are considered and compared. We additionally investigate what is considered a vital early stage in shell processing - the viability of a lactic acid fermentation on demineralised shell for deproteinisation under both aerobic and anaerobic conditions. An integrated bioconversion scheme is proposed whereby protein, pigment (astaxanthin) and the main biopolymer constituent, chitin, can be isolated for industrial or commercial use. The scheme also suggests further conversion of the chitin to its monomer, N-acetylglucosamine, for use as a sugar or as a medium source for production of microbial biomass protein.     

Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry

The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strain of the enteropathogens at 10(7) cells g(-1) dry weight. Survival data was fitted to the Weibull model. The survival curves in aerobic conditions generally showed a concave curvature, while the curvature was convex in anaerobic conditions. The estimated survival times showed that E. coli O157:H7 survived significantly longer under anaerobic than under aerobic conditions. Survival ranged from approximately. 2 weeks for aerobic manure and slurry to more than six months for anaerobic manure at 16 °C. On average, in 56.3% of the samplings, the number of recovered E. coli O157:H7 cells by anaerobic incubation of Petri plates was significantly (p < 0.05) higher in comparison with aerobic incubation. Survival of Salmonella serovar Typhimurium was not different between aerobic and anaerobic storage of LB broth or manure as well as between aerobic and anaerobic incubation of Petri dishes. The importance of changes in microbial community and chemical composition of manure and slurry was distinguished for the survival of E. coli O157:H7 in different oxygen conditions.     

Greenhouse gas balance for composting operations

The greenhouse gas (GHG) impact of composting a range of potential feedstocks was evaluated through a review of the existing literature with a focus on methane (CH(4)) avoidance by composting and GHG emissions during composting. The primary carbon credits associated with composting are through CH(4) avoidance when feedstocks are composted instead of landfilled (municipal solid waste and biosolids) or lagooned (animal manures). Methane generation potential is given based on total volatile solids, expected volatile solids destruction, and CH(4) generation from lab and field incubations. For example, a facility that composts an equal mixture of manure, newsprint, and food waste could conserve the equivalent of 3.1 Mg CO(2) per 1 dry Mg of feedstocks composted if feedstocks were diverted from anaerobic storage lagoons and landfills with no gas collection mechanisms. The composting process is a source of GHG emissions from the use of electricity and fossil fuels and through GHG emissions during composting. Greenhouse gas emissions during composting are highest for high-nitrogen materials with high moisture contents. These debits are minimal in comparison to avoidance credits and can be further minimized through the use of higher carbon:nitrogen feedstock mixtures and lower-moisture-content mixtures. Compost end use has the potential to generate carbon credits through avoidance and sequestration of carbon; however, these are highly project specific and need to be quantified on an individual project basis.     

Phosphorus flux from bottom sediments in Lake Eucha, Oklahoma

Phosphorus inputs into reservoirs include external sources from the watershed and internal sources from the reservoir bottom sediments. This study quantified sediment P flux in Lake Eucha, northeastern Oklahoma, USA, and evaluated the effectiveness of chemical treatment to reduce sediment P flux. Six intact sediment-water columns were collected from three sites in Lake Eucha near the reservoir channel at depths of 10 to 15 m. Three intact sediment and water columns from each site were incubated for 21 d at approximately 22 degrees C under aerobic conditions, and three were incubated under anaerobic conditions (N2 with 300 ppm CO2); sediment P flux was estimated over the 21 d for each core. The overlying water in the cores was bubbled with air for approximately 1 wk and then treated with aluminum sulfate (alum). The cores were incubated at approximately 22 degrees C for an additional 14 d under aerobic or anaerobic conditions, and sediment P flux after alum treatment was estimated for each core. Sediment P flux was approximately four times greater under anaerobic conditions compared with aerobic conditions. Alum treatment of the intact sediment-water columns reduced (8x) sediment P flux under anaerobic conditions. Internal P flux (1.03 and 4.40 mg m(-2) d(-1) under aerobic and anaerobic conditions, respectively) was greater than external P flux (0.13 mg m(-2) d(-1)). The internal P load (12 Mg yr(-1)) from reservoir bottom sediments was almost 25% of the external P load (approximately 48 Mg yr(-1)) estimated using a calibrated watershed model.     

Sorption and degradation of pesticides in nursery recycling ponds

Knowledge of pesticide distribution and persistence in nursery recycling pond water and sediment is critical for preventing phytotoxicity of pesticides during water reuse and to assess their impacts to the environment. In this study, sorption and degradation of four commonly used pesticides (diazinon, chlorpyrifos, chlorothalonil, and pendimethalin) in sediments from two nursery recycling ponds was investigated. Results showed that diazinon and chlorothalonil were moderately sorbed [K(OC) (soil organic carbon distribution coefficient) from 732 to 2.45 x 10(3) mL g(-1)] to the sediments, and their sorption was mainly attributable to organic matter content, whereas chlorpyrifos and pendimethalin were strongly sorbed (K(OC) > or = 7.43 x 10(3) mL g(-1)) to the sediments, and their sorption was related to both organic matter content and sediment texture. The persistence of diazinon and chlorpyrifos was moderate under aerobic conditions (half-lives = 8 to 32 d), and increased under anaerobic conditions (half-lives = 12 to 53 d). In contrast, chlorothalonil and pendimethalin were quickly degraded under aerobic conditions with half-lives < 2.8 d, and their degradation was further enhanced under anaerobic conditions (half-lives < 1.9 d). The strong sorption of chlorpyrifos and pendimethalin by the sediments suggests that the practice of recycling nursery runoff would effectively retain these compounds in the recycling pond, minimizing their offsite movement. The prolonged persistence of diazinon and chlorpyrifos, however, implies that incidental spills, such as overflows caused by storm events, may contribute significant loads of such pesticides into downstream surface water bodies.