Three new species of free-living marine nematodes from sublittoral sediments in Liverpool Bay

Three new species of free-living marine nematodes are described from sublittoral sediments in Liverpool Bay, UK. Pselionema longiseta n.sp. (Ceramonematoidea) is closest to P. longissimum Gerlach, 1952, from which it is distinguished by the longer cephalic setae and the larger amphids; Laimella filicaudata n.sp. (Chromadoroidea) is closest to L. longicauda Cobb, 1920, from which it is mainly distinguished by the much greater curvature of the spicules and the longer caudal apophyses of the gubernaculum; Pseudonchus decempapillatus n.sp. (Choanolaimoidea) is closest to P. deconincki Warwick, 1969 and is distinguished by the presence of 10, rather than 13, pre-anal supplements in the male, by the longer cephalic setae, and by the presence of numerous caudal setae.     

Studies on the sublittoral free-living nematodes of Liverpool Bay. I. The structure and distribution of the nematode populations

Data collected from a survey of the benthic fauna of Liverpool Bay (UK) have been used to study the distribution and structure, in terms of percent age dominance and percent age frequency, of the nematode populations. Cluster analysis of the faunistic data from individual stations has shown that the populations are not sharply delimited. The relative proportions of their characteristic genera are extremely variable, and apparently influenced by small differences in sediment composition. It is, thus, considered more logical to think in terms of a number of different habitats, each with certain characteristic genera, rather than in terms of a series of discrete associations. Six types of habitat are distinguished on the basis of sediment granulometry: (1) mud and sandy mud; (2) very muddy sand; (3) muddy sand; (4) muddy sand-gravel mixtures; (5) clean sand-gravel mixtures; (6) clean sand. Habitats 1, 2 and 3 were dominated by Sabatieria spp., the degree of dominance apparently being related to the percentage of silt-clay. Habitat 4 was dominated by Neochromadora sp. and Sabatieria spp. and Habitats 5 and 6 by Desmodora sp. Both generic and dominance diversity were very much lower for Habitats 1 and 2 than elsewhere.     

Two new species of Xyala (Nematoda,Monhysteroidea) from sublittoral sediments in Liverpool Bay

Two new species of Xyala (Nematoda, Monhysteroidea) are described from sublittoral sediments in Liverpool Bay, England. Xyala barbata n. sp. is distinguished mainly by the dorsally directed gubernacular apophyses and X. longicaudata n. sp. is distinguished by the long tail, the large amphids and the high, narrow labial rugae.     

Population distribution and structure of the free-living nematodes of Long Island Sound

The distribution and structure of nematode populations in 4 sedimentary environments (muds, muddy sands, fine sands and medium-coarse sands) in Long Island Sound were studied. Mean population densities were highest in muds and muddy sands. Cluster analysis suggested the presence of two basic faunistic units; a mud unit characterized by high species dominance, low species diversity and low species endemism, and a sand unit characterized by low species dominance, high species diversity and high species endemism. Species diversity in all habitats was a direct function of both species richness and equitability. Limited niche separation among deposit feeders, usually the dominant nematode trophic type in muddy sediments, is proposed as the cause for the high species dominance so often characteristic of shallow marine muds. The study afforded the opportunity to examine the quantitative and qualitative aspects of population structure in relation to environmental impact (as indicated by large differences in the heavy metal and organic carbon concentrations within each of the 4 sedimentary regimes). Within each sediment type no differences in population densities, species composition or species diversity of nematodes existed between heavily impacted and apparently non-impacted sediments, or between Long Island Sound and similar coastal regions. These findings cast doubt on (1) the use of heavy metal and organic carbon concentrations as indicators of environmental stress for marine nematodes; (2) the use of diversity indices alone as indicators of environmental deterioration; and (3) the usefulness of field monitoring studies alone for the assessment of pollution impact on marine nematodes.Contribution no. 100 from the Institute of Marine and Atmospheric Sciences, City College of New YorkCommunicated by M.R. Tripp, Newark     

Diversity of free-living marine nematodes (Enoplida) from Baja California assessed by integrative taxonomy

We used morphological and molecular approaches to evaluate the diversity of free-living marine nematodes (order Enoplida) at four coastal sites in the Gulf of California and three on the Pacific coast of Baja California, Mexico. We identified 22 morphological species belonging to six families, of which Thoracostomopsidae and Oncholaimidae were the most diverse. The genus Mesacanthion (Thoracostomopsidae) was the most widespread and diverse. Five allopatric species, genetically and morphologically differentiated, were found in two localities in the Gulf of California (M. sp1 and M. sp2) and three in the Pacific coast (M. sp3, M. sp4 and M. sp5). Overall, we produced 19 and 20 sequences for the 18S and 28S genes, respectively. Neither gene displayed intraspecific polymorphisms, which allowed us to establish that some morphological variation was likely either ontogenetic or due to phenotypic plasticity. Although 18S and 28S phylogenies were topologically congruent (incongruence length difference test, P > 0.05), divergences between species were much higher in the 28S gene. Moreover, this gene possessed a stronger phylogenetic signal to resolve relationships involving Rhabdodemania and Bathylaimus. On the other hand, the close relationship of Pareurystomina (Enchilidiidae) with oncholaimids warrants further study. The 28S sequences (D2D3 domain) may be better suited for DNA barcoding of marine nematodes than those from the 18S rDNA, particularly for differentiating closely related or cryptic species. Finally, our results underline the relevance of adopting an integrative approach encompassing morphological and molecular analyses to improve the assessment of marine nematode diversity and advance their taxonomy.     

Analysis of the small-scale spatial patterns of free-living marine nematodes from tidal flats in the East Frisian Wadden Sea

Serial samples with a multiple corer were taken in order to understand the small-scale aggregation patterns in the nematode community. A total of 77 species was found in the sample. Fifteen dominant nematode species were examined by means of a dispersion analysis. Only one species (Metachromadora scotlandica) may be considered randomly distributed. A variogram analysis gave no proof of aggregation in that species, but all the other (aggregated) species displayed different spatial patterns: (1) a maximum/aggregation at the sample's margin, (2) two maxima/aggregations in the centre of the sample, (3) aggregation of individuals distributed at random over the entire sample (Metalinhomoeus typicus) and (4) aggregation of individuals distributed at random only in the centre of the sample (Paralinhomoeus ilenensis). These results allow the prognosis that distribution patterns are repetitive in five species (Microlaimus conothelis, M. marinus, Odontophora rectangula, Oncholaimellus calvadosicus, Viscosia rustica). Finally, by means of MDS-ordination (based on cosine similarity) two nematode associations were distinguished: one group represented by diatom-feeders (sensu lato), the other one comprised of DOM-users (sensu lato). Received: 13 May 1998 / Accepted: 15 January 1999     

Free-living marine nematodes from Biscayne Bay,Florida, II. Oncholaimidae: descriptions of five new species and one new genus (Meyersia)

A new genus, Meyersia, and five new species of the nematode family Oncholaimidae, are described from Biscayne Bay, Florida, and environs, viz., Metoncholaimus pelor n. sp., M. amplus n. sp., Meyersia major n. gen., n. sp., M. minor n. sp., and Filoncholaimus prolatus n. sp. Also included in the genus Meyersia are Adoncholaimus bandaenis Kreis, 1932 and A. meridionalis Kreis, 1932.     

Distribution and abundance of marine nematodes on the kelp Macrocystic integrifolia

The species and distribution of nematodes on the kelp Macrocystic integrifolia Bory, in the Bamfield region of Barkley Sound, British Columbia, Canada were examined. Nine species (belonging to six families) of nematodes were found on the kelp blades. Three species (Monhystera disjuncta, M. refringens and Prochromadorella neapolitana) comprised 91–99% of the nematode fauna and occurred in all monthly samples from July 1978 to November 1979. The three dominant species exhibited seasonal density differences. P. neapolitana occurred mostly in the summer, M. refringens abundance peaked from July to October and M. disjuncta was relatively abundant throughout the year. The other species contributed little to the overall abundance and distribution patterns. All three species occurred in greatest abundance on the lower and middle blades of M. integrifolia in the deep end of the kelp bed. Very few individuals occurred on the upper blades. Nematode distribution on M. integrifolia appeared to be related to blade age and the associated food sources on the blade.     

Diel eclosion rhythm of a sublittoral population of the marine insect Pontomyia pacifica

Within the scope of studies on adaptation of insects to intertidal and sublittoral environments, correlations between the reproduction period of the short-lived chironomid Pontomyia pacifica Tokunaga and tidal conditions were examined at the only known Japanese location of this species in 1980. The larval habitat is situated, except for deeper tide pools in the lower midlittoral zone, mainly within the inner sublittoral zone, whose upper area is covered by Hypnea choroides, the dominating algae during the summer. The aerial adults emerge on the surface of the open sea and swarm there independent of the tidal situation above the submersed sublittoral habitat. Eclosion always started during dusk and all reproductive activities were ended within 2.5 h after sunset. The adaptation of P. pacifica (sublittoral habitat, diel eclosion after sunset with mass concentration of the adults on the water surface, no semilunar or lunar timing of the reproduction period and sinking egg masses) corresponds with those of the convergent marine chironomid Clunio balticus from Europe. On the basis of a few laboratory observations with P. pacifica and detailed experiments with C. balticus, it is supposed that the diel eclosion of P. pacifica is also controlled by an endogenous, circadian timing mechanism and the 24-h light-dark cycle as an environmental time cue.Communicated by O. Kinne, Oldendorf/Luhe     

Influence of the mineralogical composition on microbial activities in marine sediments: an experimental approach

We investigated the influence of the mineralogical composition of marine sediments on bacterial activity in experimental microcosms. Calcite and quartz were added to natural marine sediments and microbial response in terms of total bacterial abundance and biomass, β-D-glucosidase exo-enzymatic activity and bacterial incorporation of a radio-labelled (³H-leucine) substrate were investigated for a period of one month. We report here that after 15 days the mineralogical composition of the sediment (calcite vs. quartz) had an impact on bacterial abundance and activity (reduced for ca 15% and 56%, respectively). However, such impact was mitigated or even disappeared in high organic nutrient conditions.     

Studies on portunid crabs from the Eastern Pacific. II. Significance of the unusual distribution of Euphylax dovii

Euphylax dovii Stimpson (Brachyura: Portunidae: Podophthalminae), a tropical Eastern Pacific swimming crab, has distinctive morphological adaptations for pelagic existence. Crabs in collections from the open ocean had a sex ratio approximating 1:1, with no crabs bearing eggs. Samples from the continental shelf of Colombia contained thousands of females, mostly ovigerous, but no males. Egg attachment has posed a major problem in the evolution of decapod crustaceans, and the two genera of portunid crabs thus far observed cannot attach eggs unless females can bury partly in soft sediments. This suggests that mated E. dovii females must migrate into shallow shelf waters to encounter sediments necessary for spawning. The high energetic cost of swimming while carrying eggs and the presence of abundant food for larvae are factors favoring residence of females in shelf waters until hatching is complete.     

Heavy-metal distribution and risk assessment of sediment and fish from El-Mex Bay, Alexandria, Egypt

Heavy metals are increasingly being released into natural waters from geological and anthropogenic sources. The distribution of several heavy metals (Cr, Cu, Cd, Pb, Zn, and Hg) was investigated in muscle, gill, and liver in two different fish species seasonally collected in El-Mex Bay (autumn 2004-summer 2005). In order to evaluate the pollution status of the Bay, the concentrations of the selected metals in the labile and total fractions were analysed in sediment samples collected from eight sites in El-Mex Bay during autumn 2004. Also, the Index of Geoaccumulation (I_geo) for the sediment was estimated. The total and labile fractions of the selected metals in sediment samples were 15.2 and 62.8 μg g^-1 dw for Cu, 1.8 and 5.0 μg g^-1 dw for Cd, 79.1 and 130.3 μg g^-1 dw for Zn, 0.2 and 1.2 μg g^-1 dw for Hg, 35.8 and 93.0 μg g^-1 dw for Pb, and 13.9 and 31.0 μg g^-1 dw for Cr. The concentrations of all metals were lower in flesh than those recorded in liver and gill due to their physiological roles. The metal pollution index for fish was calculated. Health hazard calculations for the contaminated sediments and fish consumption were calculated to evaluate the effect of pollution on health.     

Salinity effects on the coexistence of cryptic species: a case study on marine nematodes

The coexistence of four cryptic species of Rhabditis (Pellioditis) marina (Nematoda: Rhabditidae) at small geographical scale challenges ecological competition theory and was therefore studied in the laboratory at two different salinities, where their performance in combined cultures was compared with that in monospecies cultures. We found that three of the four cryptic species were able to coexist, but that interspecific interactions (competition and facilitation) were common. Salinity had an effect on these interactions, with a shift from contest to scramble competition. This shift may result from an increased population development of two of the four species at the lower salinity in the monospecific cultures. This experiment demonstrates that abiotic conditions may play an important role in achieving coexistence between cryptic species and can alter the interspecific interactions between them.     

Free-living marine nematodes from Biscayne Bay,Florida, I. Comesomatidae: the male of Laimella longicauda Cobb, 1920, and description of Actarjania new genus

The male of Laimella longicauda Cobb, 1920 is described as having short arcuate spicules and a gubernaculum with caudally directed apophyses. The genus Paracomesoma Schuurmans Stekhoven, 1950 is reinstated to accommodate species of Laimella sensu Chitwood, 1937. Paramesonchium n. gen. is proposed to accommodate Laimella (?) serialis Wieser, 1954. Actarjania n. gen. (type species A. splendens n. sp.) differs from Sabatieria in having irregular lateral rows of longitudinal punctations and short arcuate spicules with distinctive distal extremities.     

Nutrient cycling between the water column and a marine sediment. I. Organic carbon

The carbon flow through the sediments at a station located in 18.3 m of water off the Scripps Institution of Oceanography, San Diego, California (USA) was determined. The parameters studied [and their mean rates of input (+) or output (-) to the benthos] were macro-detritus (+0.028 gC m^-2day^-1), fallout of particulate debris (+3.3 gC m^-2day^-1), benthic net photosynthesis during the day (-0.06 gC m^-2 daylight period^-1), burial (0 gC m^-2day^-1), benthic respiration at night (-0.28 gC m^-2 night period^-1), and resuspension (-3.0 gC m^-2day^-1). Resuspension of sediment at this station was found to have a controlling effect on the sediment organic carbon content. Benthic photosynthesis was able to provide 79% of the organic carbon required by the benthos for respiration during the daylight hours. A carbon-flow diagram linking together all of the above measurements is presented.     

Life history of the nematode Theristus anoxybioticus from sublittoral muddy sediment at methane seepages in the northern Kattegat,Denmark

Field and laboratory studies from 1989 to 1994 of the nematode Theristus (Penzancia) anoxybioticus Jensen, 1995 inhabiting muddy sediment at methane seepages in the northern Kattegat, Denmark, have revealed a series of biological features of adults and juveniles not observed before in free-living marine nematodes. Reproductive adults were present in April and May only and inhabited the uppermost cm of sediment, while juveniles inhabited deep anoxic and sulphidic sediment layers during the remaining months of the year. This points to a downwards migration of newly hatched juveniles and an upwards migration during adulthood. Gut content analyses showed that adults feed mainly on benthic diatoms by swallowing whole cells while juveniles probably feed on bacteria; this differentiation in food clearly relates to the main bulk of potential food items in their respective microhabitats. The generation time is estimated to be one year, with a juvenile life span of about nine months, which is four to eight times longer than that found in related aerobic species of similar body size. Adults survived in oxic water but 50% died within 15 h in hypoxic water (5 to 6% atm sat), and all died within 12 h in anoxic water. Juveniles on the other hand died within 0.5 h in oxic water but survived anoxic water for at least 15 d. These results suggest that juveniles of T. anoxybioticus are adapted to an anaerobic life style.     

Temperature-induced changes in the formation of sulphide in a marine sediment

The influence of temperature upon sulphide formation was investigated with sediment sulphureta incubated at constant temperatures in the laboratory. The concentrations of sulphate, sulphide and pyrite were periodically measured and it was found that, in addition to a decrease in the rate of sulphide formation with temperature, there was a changes in the origin of the sulphide. Thus, at 5° and 10°C, the majority of sulphide originated from organic sulphur, while sulphate contributed the greater proportion of the sulphide at 20° and 30°C. Such changes presumably reflect those in the natural enviroment during winter and summer.     

Marine calanoid resting eggs in Norway: Abundance and distribution of two copepod species in the sediment of an enclosed marine basin

Abundance and distribution of calanoid resting eggs in sediment were examined in 1988–1989 in an enclosed marine basin (Svartatjønn; western Norway). The basin undergoes rotenone treatment and draining every winter. Egg densities were highest in autumn, when up to 2 × 10⁶ eggs m^–2 were found, and lowest in summer. Egg densities were also highest in the deeper parts of the basin and in the upper 1 cm of sediment. Hatching was initiated in late January by pumping warmer deep water from the outside area into the basin. Evidence is given for resting-egg formation inEurytemora affinis Poppe, 1880 andAcartia clausi Gurney, 1931 (present phenotype reclassified asA. teclae by Bradford 1976: N. Z. Jl mar. Freshwat. Res. 10: 159–202). Seasonal differences in inhibition of hatching, together with the longevity and tolerance of these eggs, strongly suggest that they are diapause eggs.