Fecal coliform loadings and stocks in buttermilk bay,Massachusetts, USA,and management implications

Abundance of fecal caliform bacteria is a weak index of the presence of human pathogens in wastewater entering coastal waters. In spite of this, use of fecal caliform indices for management purposes is widespread. To gain insight into interpretation of fecal coliform data, we evaluated size of stocks of fecal coliforms in water, sediments, and sea wrack, in Buttermilk Bay, a coastal embayment in Massachusetts. Sediments contained most of the fecal coliforms. Fecal coliforms in sediments were as much as one order of magnitude more abundant than in the water column or in sea wrack. The fecal coliforms in sediments of Buttermilk Bay were so abundant that resuspension of fecal coliforms from just the top 2 cm of muddy sediments could add sufficient cells to the water column to have the whole bay exceed the federal limit of fecal coliforms for shellfishing. The major sources of fecal coliforms to the bay were water-fowls, surface runoff, groundwater, and streams. Waterfowl were the largest source of fecal coliforms during cold months; surface runoff, streams, and groundwater were most important during warm months. Redirection of surface runoff pipes is unlikely to be a very successful management action since contributions via this source are insufficient to account for the measured increases in concentrations of fecal coliforms in water. Removal of waterfowl is also unlikely to be useful, since fecal coliform concentrations leading to closures of shellfish beds and swimming areas are most frequent during warm months when waterfowl are rarest. Rates of loss of fecal caliform cells from the water column by death and tidal exchange were high. Mortality of cells was about an order of magnitude larger than losses by tidal exchange. The amounts of fecal coliforms brought into the bay by waterfowl, surface runoff, groundwater, and streams are an order of magnitude smaller than the losses by mortality and tidal removal. This implies that there is an additional source of fecal coliforms within the bay. We suggest that resuspension of the upper layers of sediments can easily account for the fecal coliforms present in the water. Fecal coliform content of water and shellfish were not correlated. In contrast, sediment and shellfish fecal coliform abundances were significantly related. Monitoring of fecal coliforms in sediments may provide a better assessment of shellfish than sampling of water. The large fecal coliform stock in sediments should be the first priority for management. Efforts ought to be directed toward the reduction of sediment fecal coliform stocks. Lowering nutrient additions to coastal water bodies may be one practical approach.     

Treatment and reuse of sewage sludge

Sewage sludge was treated using composting, fixed-bed and stirred anaerobic digesters. The treatment performance in terms of the physico chemical parameters, bacterial indicators and pathogenic forms were assessed. In addition, the biogas production rate was recorded in the case of anaerobic digesters. Composting of the sewage sludge increased its total solids from 39 to 93% after 6 weeks, while the reduction in organic matter was 40% and the total nitrogen and phosphorus contents increased by 22 and 30%, respectively. Complete removal of salmonellae and faecal coliforms occurred, so that the composted sludge could be used as a soil conditioner and fertilizer. The results of the anaerobic treatment indicated that an organic load of 4.8kg COD m–3 per day achieved the best operating conditions for either the stirred or fixed-bed digester. The mean percentage removals of COD, BOD, faecal coliforms, faecal streptococci and the biogas production rate for the stirred digester were 53, 53, 24 and 29% and 259 L kg–1 COD per day, respectively. The corresponding mean percentage removals and production rate for the experiments with a fixed-bed digester were 61, 62, 33 and 35% and 328 L kg–1 COD per day, respectively. Improvements in the BOD and faecal coliform reductions and the gas production rate of 17, 38 and 21%, respectively, were achieved due to the presence of media (Berl saddles) in the fixed-bed digester. The microbial content of the anaerobically treated sludge is too high to be used as a fertilizer, while that of the composted sludge is low enough for such use.     

The performance of constructed wetlands treating primary, secondary and dairy soiled water in Ireland (a review)

In Ireland, no database detailing the design, influent loading rates or performance of constructed wetlands (CWs) exists. On account of this, they are designed without any protocol based on empirical data. The aim of this paper was to provide the first published data on the performance of free-water surface flow (FWSF) CWs treating primary and secondary-treated municipal wastewater, and agricultural dairy soiled water (DSW) in Ireland. In total, the performance of thirty-four FWSF CWs, comprising fourteen CWs treating primary-treated municipal wastewater, thirteen CWs treating secondary-treated municipal wastewater, and seven CWs treating DSW, were examined. In most CWs, good organic, suspended solids (SS) and nutrient removal was measured. At an average organic loading rate (OLR) of 10 and 9 g biochemical oxygen demand (BOD) m(-2) d(-1), CWs treating primary and secondary wastewater removed 95 and 84% of influent BOD. Constructed wetlands treating DSW had an average BOD removal of 98%. At average SS loading rates of 6 and 14 g m(-2) d(-1), CWs treating primary and secondary wastewater had a 96 and an 82% reduction, and produced a final effluent with a concentration of 14 and 13 mg L(-1). Constructed wetlands treating DSW produced a final effluent of 34 mg L(-1) (94% reduction). Similar to other studies, all CWs examined had variable performance in ammonium-N (NH(4)(+)-N) removal, with average removals varying between 37% (for CWs treating secondary wastewater) and 88% (for CWs treating DSW). Variable ortho-phosphorus (PO(4)(3-)-P) removal was attributable to different durations of operation, media types and loading rates.     

CONCENTRATION AND SOURCES OF FECAL AND ORGANIC POLLUTION IN AN AGRICULTURAL WATERSHED1

ABSTRACT: Fecal contamination and organic pollution of an agricultural drainage in northeast Indiana was high. Bacterial counts (total coliform, TC; fecal coliform, FC; and fecal streptococcus, FS) and biochemical oxygen demand (BOD) were used to assess waste concentrations. Coliform counts indicated that sections of the drainage receiving septic effluent had waste concentrations far in excess of public health standards (mean FC = 550,000/100 ml). Areas of drainage remote from septic tank pollution were found to occasionally meet federal public health standards for whole body contact recreation but generally these areas had twice the allowable limit of 200 FC/100 ml. Bacterial contamination was highest during runoff events when the median values for TC, FC, and FS were 5, 3, and 17 times greater, respectively, than the median values during low stream discharge. Surface flows carried contaminants from unconfined livestock operations and fecally contaminated sediment was transported by high waters. During one runoff event a BOD loading of 36.7 kg/km² was recorded and the peak BOD concentration observed was 16 mg/l. A discharge of liquid manure from a confined livestock operation caused a major fish kill. Pollution from septic tanks and unconfined livestock is greatest at high stream discharge when dilution reduces the impact on aquatic life.     

高负荷活性污泥法应用于中小城市污水处理的研究

针对目前二级城市污水处理厂工程投资高、运行费用高的特点,提出适合中小城市污水处理的工艺——高负荷活性污泥法（HAS）。该工艺不设初次沉淀池,负荷高,泥龄短,污泥沉降性能良好,不存在污泥膨胀,对有机物的去除以絮凝、吸附、沉淀作用为主,对COD、BOD,、SS都有较高的去除效果,对氮、磷有一定的去除作用,出水水质可满足《农田灌溉水质标准》的要求。     

Reduction of pathogen indicator organisms in dairy wastewater using an ecological treatment system

Ecological treatment systems can provide a sustainable, plant-based alternative to traditional wastewater treatment. One factor essential to the success of these systems is ensuring their ability to reduce coliform concentrations in wastewater. Wastewater is the primary source of fecal contamination in aquatic ecosystems, containing total and fecal coliforms on the order of 10(8)-10(10) and 10(7)-10(9) CFU L(-1), respectively. This study assessed the ability of an ecological treatment system to reduce concentrations of total coliforms and Escherichia coli from dairy wastewater. Low strength wastewater was pumped into the system during July of 2005 and high strength in September 2005. Wastewater passes through a series of anaerobic, aerobic, and clarifier reactors and wetland cells before exiting the system. Regardless of wastewater strength, average total coliform and E. coli concentrations were consistently reduced by at least 99% from influent to effluent, with the majority of the reduction (76%) occurring in the first two reactors. Relationships between internal concentrations of solids and coliforms indicated that increased reduction of solids may further reduce coliform concentrations. Although U.S. Environmental Protection Agency discharge requirements for E. coli were not always met, the substantial reductions achieved indicate that ecological treatment systems have the potential to successfully reduce coliforms in wastewater to meet discharge limits. The results from this study will be used to guide design and management of future ecological treatment systems, so that larger and more consistent coliform reductions can be achieved.     

Assessment of AWT systems in the metro Atlanta area

A study was conducted to evaluate the performance of six advanced wastewater treatment facilities using biological nutrient removal processes as the primary mechanism for removing carbon, nitrogen, and phosphorus from domestic wastewater. One year of operating data was obtained from monthly operating reports provided by the Georgia Environmental Protection Division (EPD) in Atlanta. Additional information about facility operations and the types of chemicals used was gathered through review of EPD files and interviews with plant personnel. Data evaluated were: influent and effluent five-day Biochemical Oxygen Demand (BOD(5)); influent and effluent total suspended solids (TSS); influent Total Kjeldahl Nitrogen and effluent Total Nitrogen; and influent and effluent Total Phosphorus (TP). Although varying from plant to plant, effluent requirements for BOD(5), TSS, ammonia, and TP were met. Chemicals utilized, design capacity, and monthly effluent concentrations are presented in this study.     

AN ANALYSIS OF COLIFORM BACTERIA IN THE UPPER ILLINOIS WATERWAY1

ABSTRACT: During the summer of 1971 about 150 water samples were examined for total and fecal coliform bacteria in the Upper Illinois Waterway at 19 river stations. The data per station were found to be normal geometric distributed. Bacteria densities changed with sampling dates and generally decreased with water movement downstream. Several sewage treatment effluents made marked pulses along the bacterial die-off curves. The observed fecal coliform results were evaluated in terms of the Illinois Pollution Control Board's standards. The FC:TC ratio on the waterway for each station were presented. Using Chick's Law, coliforms death rates were estimated. Efforts to correlate observed total and fecal densities with temperature, flow, algal densities, dissolved oxygen, and 5-day biochemical oxygen demand were not successful. (KEY TERMS: algae; biochemical oxygen demand; coliform bacteria; dissolved oxygen; flow; stream survey; temperature; water pollution; water quality standards)     

Performance assessment of a wastewater treatment plant producing effluent for irrigation in Egypt

A conventional activated sludge treatment facility was the subject of this study. The assessment was directed at determining the characteristics of the raw wastewater, the quality of the treated effluent and the efficiency of the various treatment units. Furthermore, the water quality along the effluent irrigation canal was monitored. The assessment of the quality of the treated effluent for irrigation is based on the guidelines established by the World Health Organization and the Egyptian decree 9/89 for the use of wastewater in agriculture. The results of the study indicated that the concentration of the raw wastewater was considered moderate. The mean values of the COD (Chemical Oxygen Demand), BOD (Biological Oxygen Demand) and total suspended solids (TSS) were around 250, 102 and 142 mg l–1, respectively. This was attributed to the high quantities of wastewater from industrial sources. The overall efficiency of the treatment facility was good. The mean residual COD, BOD and TSS were 25, 8 and 21 mg l–1 and the corresponding percentage removal values were 90, 92 and 85%, respectively. The maximum percentage removal of oil and grease was 84% with a mean residual concentration of 24 mg l–1. The total viable count (22°C and 37°C), faecal coliform and aecal streptococci were reduced by 99.9% compared to only 99.5% for Salmonella. Bacteriological examination of the dried sludge indicated a reduction of nine logs of faecal coliform and faecal streptococci, as compared to thickened sludge. Analysis of the Ni, Cu, Pb and Cr in the dried sludge indicated that their concentrations are within the permissible limits. Zinc exceeded the consent standards by 50%. The results of the analyses of samples collected at the beginning of the irrigation canal indicated insignificant changes from the characteristics of the final effluent. Samples collected at a distance of 2km along the irrigation canal showed mean reductions in the COD and BOD of 28.6 and 47%, respectively, which could be attributed to sedimentation and/or a self-purification effect. An increase in the total nitrogen, total phosphorus and total viable count was also recorded, which could be due to seepage from the agricultural land. From the data available it is evident that the treated wastewater could be used for restricted irrigation. The design and implementation of a monitoring programme is recommended.     

Evaluating Microbial Indicators of Environmental Condition in Oregon Rivers

Traditional bacterial indicators used in public health to assess water quality and the Biolog system were evaluated to compare their response to biological, chemical, and physical habitat indicators of stream condition both within the state of Oregon and among ecoregion aggregates (Coast Range, Willamette Valley, Cascades, and eastern Oregon). Forty-three randomly selected Oregon river sites were sampled during the summer in 1997 and 1998. The public health indicators included heterotrophic plate counts (HPC), total coliforms (TC), fecal coliforms (FC) and Escherichia coli (EC). Statewide, HPC correlated strongly with physical habitat (elevation, riparian complexity, % canopy presence, and indices of agriculture, pavement, road, pasture, and total disturbance) and chemistry (pH, dissolved O2, specific conductance, acid-neutralizing capacity, dissolved organic carbon, total N, total P, SiO2, and SO4). FC and EC were significantly correlated generally with the river chemistry indicators. TC bacteria significantly correlated with riparian complexity, road disturbance, dissolved O2, and SiO2 and FC. Analyzing the sites by ecoregion, eastern Oregon was characterized by high HPC, FC, EC, nutrient loads, and indices of human disturbance, whereas the Cascades ecoregion had correspondingly low counts of these indicators. The Coast Range and Willamette Valley presented inconsistent indicator patterns that are more difficult to characterize. Attempts to distinguish between ecoregions with the Biolog system were not successful, nor did a statistical pattern emerge between the first five principle components and the other environmental indicators. Our research suggests that some traditional public health microbial indicators may be useful in measuring the environmental condition of lotic systems.     

Modeling manure-borne bromide and fecal coliform transport with runoff and infiltration at a hillslope

Hillslope vegetated buffers are recommended to prevent water pollution from agricultural runoff. However, models to predict the efficacy of different grass buffer designs are lacking. The objective of this work was to develop and test a mechanistic model of coupled surface and subsurface flow and transport of bacteria and a conservative tracer on hillslopes. The testing should indicate what level of complexity and observation density might be needed to capture essential processes in the model. We combined the three-dimensional FEMWATER model of saturated-unsaturated subsurface flow with the Saint-Venant model for runoff. The model was tested with data on rainfall-induced fecal coliforms (FC) and bromide (Br) transport from manure applied at vegetated and bare 6-m long plots. The calibration of water retention parameters was unnecessary, and the same manure release parameters could be used both for simulations of Br and FC. Surface straining rates were similar for Br and bacteria. Simulations of Br and FC concentrations were least successful for the funnels closest to the source. This could be related to the finger-like flow of the manure from the strip along the bare slopes, to the transport of Br and FC with manure colloids that became strained at the grass slope, and to the presence of micro-ponds at the grassed slope. The two-dimensional model abstraction of the actual 3D transport worked well for flux-averaged concentrations. The model developed in this work is suitable to simulate surface and subsurface transport of agricultural contaminants on hillslopes and to evaluate efficiency of grass strip buffers, especially when lateral subsurface flow is important.     

Evaluation of municipal wastewater treatment plants with different technologies at Las Rozas, Madrid (Spain)

Eight small-scale municipal wastewater treatment plants were evaluated over a period of 19 months in the suburb of Las Rozas in Madrid (Spain). Four plants used compact extended aeration, two used conventional activated sludge, two used conventional extended aeration, one used a rotary biodisc reactor and the other used a peat bed reactor. The best results were obtained from the plants that used conventional technologies and the biodisc. Conventional activated sludge and extended aeration had higher removal efficiencies for ammonia, TSS, COD and BOD(5) and produced good quality final effluents for final disposal in accordance with the discharge standard. Empirical equations that correlated the concentration of dissolved oxygen in the effluents with the efficiencies of TSS, ammonia, COD and BOD(5) removals for all plants evaluated were obtained. The performance of the plants using compact extended aeration was affected more than those using conventional technologies or rotary biodisc when the capacity exceeded that of its initial design.     

The effect of environmental conditions on the ability of a constructed wetland to disinfect municipal wastewaters

Constructed wetlands are widely used all over the world for the treatment of municipal wastewaters, which are characterized by high concentrations of pathogens. The objectives of this study were (1) to study the effect of solar radiation and temperature on the ability of a constructed wetland to reduce the concentration of total coliforms (TC), and (2) to evaluate the relationship between the presence of Salmonella spp. in the outflow and the concentration of TC. The results of this study showed that under Mediterranean environmental conditions, the percentage reduction in coliforms was lower during winter compared to all other seasons. Maximum removal of coliforms was achieved under conditions of high solar radiation and temperature. In addition, solar radiation was found to play a greater role in coliform die-off at low temperatures than at high temperatures. Finally, it was found that the probability of Salmonella spp. appearance in the outflow of the wetland was related to the concentration of TC. The increase in coliform bacteria in the effluents also increased the chances of Salmonella appearance. The risk of Salmonella spp. appearance in the outflow is minimized when the concentration of TC is below 10²/100 mL.     

Multiple pollutant discharge permit markets,a challenge for wastewater treatment plants

This study focuses on the challenge of using a multiple pollutant transferable discharge permit market for operating wastewater treatment plants. It uses an analytical case of Sefidrud River in Iran with two checkpoints. It shows that the operating limitations for simultaneous biochemical oxidation demand (BOD) and total nitrogen (TN) removal may convert the economically optimal waste load allocation (WLA) to a framework with lack of incentives. Therefore, water quality trading (WQT) may lose its economical advantages. In this case, a third parameter, such as dissolved oxygen is recommended as an index for assigning market interactions. In spite of economical and practical success, this approach made WLA become a more complicated and uncertain policy. It was totally concluded that using single parameter WQT is only recommended for areas with small agricultural activities or lakes. Otherwise, the integrated discharged permit and reclaimed water market is proposed instead for simultaneous BOD and TN management.     

多管发酵法与其改进法检测水中粪大肠菌群的比较

粪大肠菌群是水体中粪便污染的重要指示菌,对于评价水质污染程度具有重要的卫生学意义。多管发酵法是传统的粪大肠菌群检测方法,但其步骤繁杂、周期长。目前,已有部分监测及科研人员对其进行了改进,笔者采用改进法与国标多管发酵法进行比较验证,认为Ec培养法可完全替代国标多管发酵法,纸片法亦可用于除酸性水样及菌量大的原污水外的其他环境水样中粪大肠菌群的检测。     

Prospects for a self-sustainable sewage treatment system: a case study on full-scale UASB system in India's Yamuna River Basin

The government of India decided to launch a project to implement 16 full-scale Upflow Anaerobic Sludge Blanket (UASB) reactors (with a total capacity of 598,000 m(3)/d) in the Yamuna River basin under its Yamuna Action Plan (YAP). A polishing pond called the Final Polishing Unit (FPU) was utilized for post-treatment. This paper evaluates the sewage treatment efficiency of the combined system of full-scale UASB reactors and polishing ponds under Indian climatic conditions. Results have shown that the effluent from the sewage treatment plants (STPs) investigated failed to comply with applicable discharge standards in terms of BOD, SS, and fecal coliform removal. Therefore, it is proposed that such proper operation and maintenance as removing excess sludge and scum be conducted in order to increase treatment efficiency. Moreover, trained and experienced workers are also required to operate and maintain the systems, along with a scientific approach.     

Fate of pathogenic microorganisms and indicators in secondary activated sludge wastewater treatment plants

This study was undertaken to investigate the removal of pathogenic microorganisms and their indicators in a laboratory scale biological treatment system that simulated the secondary treatment process of a wastewater treatment plant (WWTP). Four groups of microorganisms including bacteria, viruses, protozoa and helminths as well as the selected indicators were employed in the investigation. The results demonstrated that approximately 2-3 log10 removal of the microbial indicators was achieved in the treatment process. The log removal of Clostridium perfringens spores was low due to their irreversible adsorption to sludge flocs. The laboratory treatment system demonstrated a similar removal capability for Escherichia coli and the bacterial indicators (total coliforms, enterococci and particles <2.73 microm/L). The MS-2 bacteriophage, measured as a viral indicator, showed a lower removal than poliovirus, which may be considered as a worst case scenario for virus removal. The results of using particle profiling as an indicator for protozoa and helminths appeared to be inaccurate. The removal performance for bacterial and protozoan pathogens and their indicators in a full scale WWTP and the laboratory treatment system was compared.     

Application of a new type of moving bio-film in aerobic sequencing batch reactor (aerobic-SBR)

A moving bio-film (MB), made from the inner tube of used tyres was applied in a conventional-aerobic-SBR for increasing the system efficiency and quality of bio-sludge due to good sedimentation (the density of 1.925+/-0.21 g/cm(3)), non-biodegradability and re-usability of the media without any regeneration. The total bio-sludge mass of the MB-aerobic-SBR was about 30% higher than that of the conventional-aerobic-SBR resulting in a reduction of the F/M value of the system and amount of suspended bio-sludge waste. The amount of suspended bio-sludge waste, SVI and SRT of the MB-aerobic-SBR under a low organic loading of 80+/-9.3g BOD(5)/m(3)-d were 1,485+/-146 mg/d, 51+/-3.7 ml/g and 10.1+/-5.1 days, respectively while they were 1,800+/-152 mg/d, 69+/-4.0 ml/g and 8.3+/-5.3 days, respectively in the conventional-aerobic-SBR. The BOD(5), TKN and TP removal efficiencies of the MB-aerobic-SBR were about 1-2, 2-3 and 10-12% higher, respectively, than that of the conventional-aerobic-SBR. Also, the BOD(5) and COD removal efficiencies of the MB-aerobic-SBR were higher than 95% even when the system was operated with synthetic wastewater containing 800 mg/l BOD(5) under a very low HRT of 1.5 days (organic loading of 528+/-50.8 g BOD(5)/m(3)-d). The effluent BOD(5), COD, total kjeldahl nitrogen, total phosphorus and suspended solids of the MB-aerobic-SBR under a high organic loading of 528+/-50.8 g BOD(5)/m(3)-d were 45+/-5.1, 37+/-3.6, 4.1+/-1.0, 1.5+/-0.80 and 41+/-2mg/l, respectively.     

Evaluating microbial purification during soil treatment of wastewater with multicomponent tracer and surrogate tests

Soil treatment of wastewater has the potential to achieve high purification efficiency, yet the understanding and predictability of purification with respect to removal of viruses and other pathogens is limited. Research has been completed to quantify the removal of virus and bacteria through the use of microbial surrogates and conservative tracers during controlled experiments with three-dimensional pilot-scale soil treatment systems in the laboratory and during the testing of full-scale systems under field conditions. The surrogates and tracers employed included two viruses (MS-2 and PRD-1 bacteriophages), one bacterium (ice-nucleating active Pseudomonas), and one conservative tracer (bromide ion). Efforts have also been made to determine the relationship between viruses and fecal coliform bacteria in soil samples below the wastewater infiltrative surface, and the correlation between Escherichia coli concentrations measured in percolating soil solution as compared with those estimated from analyses of soil solids. The results suggest episodic breakthrough of virus and bacteria during soil treatment of wastewater and a 2 to 3 log (99-99.9%) removal of virus and near complete removal of fecal coliform bacteria during unsaturated flow through 60 to 90 cm of sandy medium. Results also suggest that the fate of fecal coliform bacteria may be indicative of that of viruses in soil media near the infiltrative surface receiving wastewater effluent. Concentrations of fecal coliform in percolating soil solution may be conservatively estimated from analysis of extracted soil solids.     

污泥龄对剩余污泥减量化的影响

为了解决污水厂生化系统剩余污泥产量过多的问题,采用延长污泥龄(SRT)的方法,对剩余污泥减量化的影响进行了研究。实验得知:污泥龄由8d增至16d时,污泥表观产率系数下降28.53%,而污泥龄由8d增至24d时,污泥表观产率系数下降51.12%。污泥龄的延长对CODCr的去除能力没有明显的影响,但使出水NH3-N浓度和TP浓度均升高,通过调整碳氮磷源的比例降低出水NH3-N和TP浓度。实验结果表明:SRT的延长,不仅降低活性污泥工艺中的剩余污泥的产量,同时还能降低处理与处置的费用,有发展前景,值得深入研究。