Determination of organic micropollutants in rain water for laboratory screening of air quality in urban environment

Rain water may collect organic air pollutants, such as polycyclic aromatic hydrocarbons (PAHs), phthalate ester (PEs), pesticides, and polychrorinated biphenyls (PCBs), that could be present in air in consequence of different factors. The determination of these compounds in rain water samples consequently may be interesting for a first screening of air quality in urban and/or industrial areas. For this purpose, 3 areas of the city of Rieti (central Italy) were selected for rain water sampling: a) the city center, densely populated and with high road traffic; b) a suburban residential area; c) the industrial area, about 3 km out of the city center and along the state road. Wet-only precipitation collectors were used for sampling; solid-phase microextraction or liquid-liquid microextraction techniques followed by GC/MS determinations were applied for sample analysis. PCBs and pesticides were never found in the samples collected. On the contrary, some polycyclic aromatic hydrocarbons and phthalates were always found. Significant and seasonal differences in PAH levels were observed in the 3 different sampling areas, with higher values always found in the city center and during the winter period; on the contrary, PEs levels remained unchanged.     

Investigation of volatile organic compounds and phthalates present in the cabin air of used private cars

The presence of selected volatile organic compounds (VOCs) including aromatic, aliphatic compounds and low molecular weight carbonyls, and a target set of phthalates were investigated in the interior of 23 used private cars during the summer and winter. VOC concentrations often exceeded levels typically found in residential indoor air, e.g. benzene concentrations reached values of up to 149.1 µg m^− 3. Overall concentrations were 40% higher in summer, with temperatures inside the cars reaching up to 70 °C. The most frequently detected phthalates were di-n-butyl-phthalate and bis-(2-ethylhexyl) phthalate in concentrations ranging from 196 to 3656 ng m^− 3.     

The use of extraction chromatography resins to concentrate actinides and strontium from soil for radiochromatographic analyses

An analytical technique utilizing selective extractant resins to concentrate strontium and actinides from soil followed by separation with radiochromatography was evaluated. The technique was tested using uncontaminated soil samples spiked with a radionuclide tracer solution that were either microwave-aided acid digested or leached with a strong acid. Extraction of the strontium and actinides from the acidified solution was accomplished using a serial arrangement of Sr-Resin and TRU-Resin columns. The combined eluate solutions from the extraction resins were treated with HNO(3) and H(2)O(2) to oxidize residual extractant and eluates prior to separation and analysis of the radionuclides by radiochromatography. Chromatograms obtained with larger soil mass loadings resulted in either incomplete peak resolution of the tracers or had highly variable peak elution times, indicative of an ionic interfering constituent(s). Better separations (e.g., chromatograms that resolved all radioactive constituents) were obtained when the sample mass loading was decreased, but with a concurrent decreased sensitivity for the radionuclides. Elemental analyses of the soil were conducted to provide data on the ionic constituents in unprocessed soil and post-processed soil samples. These results identified aluminum as an interfering contributor to the poor performance exhibited by the radiochromatographic separations.     

Fate of oil from two major oil spills: Role of microbial degradation in removing oil from the Amoco Cadiz and IXTOC I spills

The fate of oil was examined in intertidal sediments impacted by the Amoco Cadiz spill in Brittany, France, and in surface waters contaminated by the IXTOC I well blowout in the Bay of Campeche, Mexico. The role of microbial degradation in removing the contaminating hydrocarbons was assessed by laboratory determinations of numbers of hydrocarbon utilizing microorganisms, measurement of microbial hydrocarbon biodegradation potentials, by chemical analyses of samples incubated under controlled conditions and by examination of the evolution of the hydrocarbon mixture in field samples collected after the spill. Analyses included the use of ¹⁴C radiolabelled hydrocarbon tracers, glass capillary-gas chromatography and mass spectrometry. Numbers of hydrocarbon utilizing microorganisms were found to be elevated by several orders of magnitude in oil contaminated samples. Numbers of hydrocarbon utilizing microorganisms appear to provide a useful index of environmental hydrocarbon contamination. However, environmental constraints on microbial processes appear to be most important in determining actual rates of biodegradation rather than numbers of available hydrocarbon utilizing microorganisms. Very high rates of degradation were found in intertidal sediments impacted by the Amoco Cadiz spill. In contrast, rates of degradation in the Gulf of Mexico were found to be much lower, partly due to nutrient limitations and partly due to the formation of large masses of oil-water emulsion or mousse.     

Monitoring individual exposure. Measurements of volatile organic compounds in breathing-zone air, drinking water, and exhaled breath

Methods for determining individual exposure to volatile organic compounds (VOC) during normal daily activities were field tested on university student volunteers in Texas and North Carolina. The equipment tested included a personal monitor employing Tenax GC® to collect organic vapors for later analysis by GC-MS, and a specially designed spirometer for collecting samples of expired human breath on duplicate Tenax cartridges. The personal monitor and spirometer proved feasible for collecting abundant quantitative data on most of the 15 target organics. Air exposures to many VOC varied widely, sometimes over three orders of magnitude, among students on the same campus who had been monitored over the same time period and day. A log-linear relationship between breathing-zone air exposures and concentrations in exhaled breath was suggested for three chemicals: tetrachloroethylene, 1,1,1-trichloroethane, and vinylidene chloride. Air was the main route of exposure for all target compounds except the two trihalomethanes (chloroform and bromodichloromethane), which were transmitted mainly through water. Estimated total daily intake through air and water of the target organics ranged from 0.3 to 12.6 mg, with 1,1,1-trichloroethane at the highest concentrations in both geographic areas.     

Bioaccumulation of lead in Xenopus laevis tadpoles from water and sediment

The overall objective of this research was to monitor the uptake kinetics of lead in an amphibian model and correlate metal content with embryo development. Based upon the concentration of lead found in the water and sediment of a Louisiana swamp adjacent to a Superfund site, a controlled laboratory experiment exploring lead uptake from water and sediment by Xenopus laevis tadpoles was conducted. For 5 weeks, tadpoles were exposed to water and a simulated sediment, kaolin, spiked with 1, 5, or 10 times the concentration of lead found in field water and sediment samples. Additionally, organisms were exposed to the 5 x condition for 3 and 6 weeks. The experimental controls consisted of unexposed tadpoles and ones exposed to lead originating from water or sediment exclusively. At the end of the exposure periods, developmental data, i.e., body weight and developmental stage, were recorded, and the tadpoles were analyzed for whole body lead concentration. Lead extraction was accomplished by dry ashing, and its amount was quantified polarographically. Results showed that lead inhibited the normal development of these amphibians, in a manner that generally was more severe as exposure level increased. The hindrance of tadpole development also coincided with an increase in whole body lead concentration at higher exposures. Temporally, at the 5 x exposure concentration, the mean lead level increased with time, but this difference was not statistically significant (P<.05). Additionally, control animals exposed to lead (either in water or in sediment) showed no statistical difference with regard to weight and lead uptake, indicating that lead originating from both water and sediment is incorporated into the tadpole. The controlled laboratory experimental protocol used here is thus capable of investigating the uptake of a single metal (Pb in this case) and determining its effect on the development of tadpoles while differentiating the significance of multiple sources of exposure.     

Comparative evaluation of cellulose nanocrystals from bagasse and coir agro-wastes for reinforcing PVA-based composites

Environment, Development and Sustainability - In order to increase resilience of planters against climate change and bring additional economic benefits, agro-wastes can be exploited for extracting...     

Transdisciplinary research in support of land and water management in China and Southeast Asia: evaluation of four research projects

Transdisciplinary research (TDR) aims at identifying implementable solutions to difficult sustainability problems and at fostering social learning. It requires a well-managed collaboration among multidisciplinary scientists and multisectoral stakeholders. Performing TDR is challenging, particularly for foreign researchers working in countries with different institutional and socio-cultural conditions. There is a need to synthesize and share experience among researchers as well as practitioners regarding how TDR can be conducted under specific contexts. In this paper, we aim to evaluate and synthesize our unique experience in conducting TDR projects in Asia. We applied guiding principles of TDR to conduct a formative evaluation of four consortium projects on sustainable land and water management in China, the Philippines, and Vietnam. In all projects, local political conditions restricted the set of stakeholders that could be involved in the research processes. The set of involved stakeholders was also affected by the fact that stakeholders in most cases only participate if they belong to the personal network of the project leaders. Language barriers hampered effective communication between foreign researchers and stakeholders in all projects and thus knowledge integration. The TDR approach and its specific methods were adapted to respond to the specific cultural, social, and political conditions in the research areas, also with the aim to promote trust and interest of the stakeholders throughout the project. Additionally, various measures were implemented to promote collaboration among disciplinary scientists. Based on lessons learned, we provide specific recommendations for the design and implementation of TDR projects in particular in Asia.     

Nitrate in drinking water and risk of death from colon cancer in Taiwan

The relationship between nitrate levels in drinking water and colon cancer has been inconclusive. A matched case-control and a nitrate ecology study were used to investigate the association between colon cancer mortality and nitrate exposure from Taiwan's drinking water. All colon cancer deaths of Taiwan residents from 1999 through 2003 were obtained from the Bureau of Vital Statistics of the Taiwan Provincial Department of Health. Controls were deaths from other causes and were pair matched to the cases by sex, year-of-birth, and year-of-death. Each matched control was selected randomly from the set of possible controls for each case. Data on nitrate-nitrogen (NO3-N) level of drinking water throughout Taiwan have been collected from Taiwan Water Supply Corporation (TWSC). The municipality of residence for cases and controls was assumed to be the source of the subject's nitrate exposure via drinking water. The adjusted odds ratios for colon cancer death for those with high NO3-N levels in their drinking water, as compared to the lowest tertile, were 0.98 (0.84-1.14) and 0.98 (0.83-1.16), respectively. The results of the present study show that there was no statistically significant association between NO3-N in drinking water at levels in this study and risk of death from colon cancer.     

A new generic sub-model for radionuclide fixation in large catchments from continuous and single-pulse fallouts, as used in a river model

This paper presents a new general sub-model for fixation in catchment areas to be used within the framework of a river model for substances such as radionuclides and metals from continuous and single-pulse fallouts. The model has been critically tested using data from 27 European river sites covering a very wide geographical area and contaminated by radiocesium and radiostrontium from the Chernobyl accident and from the nuclear weapons tests (NWT fallout). This modelling approach gives radionuclide concentrations in water (total, dissolved and particulate phases) at defined sites on a monthly basis. The overall river model is based on processes in the upstream river stretch and in the catchment area. The catchment area is differentiated into inflow (approximately dry land) areas and outflow (approximately wetland) areas. The model has a general structure, which can be used for all radionuclides or substances. It is simple to apply in practice since all driving variables may be readily accessed from maps and standard monitoring programs. The driving variables are: latitude, altitude, catchment area, mean annual precipitation and fallout. Note that for large catchments, this model does not require data on the characteristic soil type or the percentage of outflow areas (wet lands) in the catchment, as in most previous models, since in practice it is very difficult to obtain reliable data on characteristic soil type or percentage of outflow areas, especially in large and topographically complex catchments. Modelled values have been compared to empirical data from rivers sites covering a wide domain (catchment areas from 3000 to 3,000,000 km2, precipitation from 400 to 1700 mm/year; fallouts from 1600 to 280,000 Bq/m2; altitudes from 0 to 1000 m.a.s.l. and latitudes from 41 degrees to 72 degrees N). The river model with its sub-model for fixation predicts close to the uncertainty factors given by the empirical data, which have been shown to be about a factor of 1.6 for 137Cs and a factor of 2.2 for 90Sr in river water. The obtained characteristic uncertainty factors for 137Cs from the Chernobyl fallout is 2.4, for 137Cs from the NWT fallout it is 1.3 and for the 90Sr results from the NWT fallout it is 3 using the new model.     

Residues and source identification of persistent organic pollutants in farmland soils irrigated by effluents from biological treatment plants

Sewage and industrial effluents from biological treatment plant have been widely used for agricultural irrigation in north part of China. However, effluents after biological treatment still contain heavy metals and persistent organic contaminants. The persistent organic contaminants accumulated in soil may transfer through the food chains and cause adverse health effects on human or biological effects on soil fauna and flora after long-term application. In present study, field surveys were carried out in the farmlands irrigated by effluents from biological treatment plants that receive sewage wastewater and industrial discharges. Residues of polycyclic aromatic hydrocarbons (PAHs) and organochlorine pesticides (OCPs) in the soils irrigated using both ground water and effluents were compared. The origins of PAHs in the soils were discussed. The results showed that wastewater irrigation could cause accumulation of PAHs in soils close to the pollution discharge. Significantly higher concentrations of PAHs were observed in the sampling sites close to the entrance of main channel in contrast to those along branches and the reference sites. There was no significant relationship between the accumulation of persistent organic pollutants and organic matter content in soil (TOC). Soil contamination of these persistent organic pollutants as affected by effluent irrigation was characterized by the dominant accumulation of high-molecular-weight PAHs (HMW-PAHs). In the case study, concentration of benzo[a]pyrane (BaP, 45.6 ng/g), indeno[1,2,3-cd]pyrene (IcP, 86.3 ng/g), benzo[g,h,i]perlene (BgP, 66.9 ng/g) could exceed the limits of the soil quality standard for biodegraded soils. In identification of the sources, the IcP/BgP values of PAHs in soils were more close to that in air particulates from coal/coke source (1.09+/-0.03 ng/g) [Dickhut RM, Canuel EA, Gustafson KE, Liu K, Arzayus KM, Walkers E, et al. Automotive sources of carcinogenic polycyclic aromatic hydrocarbons associated with particulate matter in the chesapeake bay region. Environ Sci Technol 2000;34:4635-40]. Therefore, both of the PAHs residues in effluents and emission from a nearby coal/coke plant were responsible. Also in this case study, low levels of the OCPs were observed and were not of significant concern in this wastewater irrigation area. Among the different OCPs analyzed, DDTs (mean 8.41 ng/g) and HCHs (mean 2.91 ng/g) were the major components. From the ratios of DDT/DDTs and beta-HCH/HCHs, it indicated that OCPs residues should be from historical usage.     

Distribution of polybrominated diphenyl ethers (PBDEs) and other persistent organic pollutants in human serum from Greece

Human serum samples (n=61) were collected in Attika, Greece between June and October 2007 and analyzed for polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCD), polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs). Thirty samples were collected from computer clerks of a large computer company in Athens working full-time with computers, and thirty-one from a control population in the Attika region with no computer use. Σ(5)PBDE concentrations (sum of tri- to hexa-BDEs) in all samples (n=61) ranged from 0.68 to 13.3 ng g(-1) lipid, with a median of 1.07 ng g(-1) lipid. These concentrations are on the lower end of those reported from other countries, probably reflecting lower usage of PBDE-containing products or lower exposures to these chemicals. Individual and Σ(5)PBDE concentrations did not statistically differ between the two groups of computer clerks and non-computer users (p>0.05), with the exception of BDE 153 (p=0.033). The predominant congener was BDE 153, followed by BDEs 47, 100, 99, 183, 154 and 28. HBCD was also detected in 70% of the samples. BDE 209 was detected in 8 out 61 samples (13%), with concentrations ranging from 1.18 to 19.1 ng g(-1) lipid, and a median of 2.94 ng g(-1) lipid. No age dependency was found for PBDEs. Σ(11)PCB (sum of PCBs 74, 99, 118, 138, 146, 153, 156, 170, 180, 183 and 187,) in all samples (n=61) ranged from 36 to 402 ng g(-1) lipid, with a median of 110 ng g(-1) lipid. pp'-DDE concentrations ranged from 53.8 to 1649 ng g(-1) lipid, with a median of 268 ng g(-1) lipid. This is the first study to report levels of PBDEs in a possibly occupationally exposed subset of the Greek population.     

Integrated phytobial remediation for sustainable management of arsenic in soil and water

Arsenic (As), cited as the most hazardous substance by the U.S. Agency for Toxic Substance and Disease Registry (ATSDR, 2005), is an ubiquitous metalloid which when ingested for prolonged periods cause extensive health effects leading to ultimate untimely death. Plants and microbes can help mitigate soil and groundwater As problem since they have evolved elaborate detoxification machineries against this toxic metalloid as a result of their coexistence with this since the origin of life on earth. Utilization of the phytoremediation and bioremediation potential of the plants and microbes, respectively, is now regarded as two innovative tools that encompass biology, geology, biotechnology and allied sciences with cutting edge applications for sustainable mitigation of As epidemic. Discovery of As hyperaccumulating plants that uptake and concentrate large amounts of this toxic metalloid in their shoots or roots offered new hope to As phytoremediation, solar power based nature's own green remediation. This review focuses on how phytoremediation and bioremediation can be merged together to form an integrated phytobial remediation which could synergistically achieve the goal of large scale removal of As from soil, sediment and groundwater and overcome the drawbacks of the either processes alone. The review also points to the feasibility of the introduction of transgenic plants and microbes that bring new hope for more efficient treatment of As. The review identifies one critical research gap on the importance of remediation of As contaminated groundwater not only for drinking purpose but also for irrigation purpose and stresses that more research should be conducted on the use of constructed wetland, one of the most suitable areas of application of phytobial remediation. Finally the review has narrowed down on different phytoinvestigation and phytodisposal methods, which constitute the most essential and the most difficult part of pilot scale and field scale applications of phytoremediation programs.