Changes in microflora of a puffer fish Fugu niphobles,with different water temperatures

Grass puffer Fugu niphobles, captured in November 1986 at Shimoda, Japan, and then reared at different temperatures ranging from 10 to 29°C, was examined for microflora changes in the skin, gill and intestines. At 10°C, the skin and gill were colonized mainly by Pseudomonas, Flavobacterium and Moraxella, while at 29°C Vibrio and Flavobacterium appeared abundantly. The intestinal microflora, consisting of Vibrio, Pseudomonas and Flavobacterium, revealed little temperature dependence, although the intrageneric composition of Vibrio changed conspicuously depending on the change of water temperature. Vibrio group 1, tentatively identified as V. alginolyticus, was detected in all tissues examined at 20 and 29°C. This, along with the fact that V. alginolyticus produces tetrodotoxin, suggests that the tetrodotoxin contained in puffer is, at least partly, accounted for by this bacterial species inhabiting intestines.     

Increased seawater temperature and light during early springs accelerate receptacle growth of Fucus vesiculosus in the northern Baltic proper

In many temperate estuarine areas, ongoing climatic changes are expected to lead to higher seawater temperatures, to increased inflow of freshwater and nutrients (due to increased rainfall) and to altered light conditions. During the last two decades, several winters have been unusually mild in southern Finland, and the subsequent springs have been early. This may have consequences for the seasonality of many species, including the bladder-wrack, Fucus vesiculosus, whose reproductive cycle may be especially sensitive. In the present study, F. vesiculosus receptacles from the field were weighed regularly during three warm (early) springs and compared with receptacles from three cold (late) springs. On average, the first sign of receptacle growth in the field occurred 5–6?weeks earlier and receptacles probably matured 2–3?weeks earlier during the warm springs than during the cold springs. This may be due to differences in seawater temperatures among years, but significantly heavier receptacles at 0.8?m compared to at 3.1?m further suggests the importance of light for receptacle growth as a measure of maturation, since there were no depth-related differences in seawater temperatures. Also, when plotting receptacle wet weights against seawater temperatures, the importance of light can be seen as different regression relationships (slopes and intercepts). In an outdoor aquarium experiment, the effects of temperature and light on receptacle growth were also tested, and there were significant differences between shallow and deep specimens, but temperature and light had no effects, except on initial receptacle growth. Since oogonian development, and thereby the time for zygote release, followed the receptacle growth curve, this could mean a mismatch between the timing of F. vesiculosus zygote settlement and the cover of competing filamentous algae, but such species interactions require further investigations in order to better understand their possible consequences.     

Identification of sex and parasitism via pheromones by the Ozark zigzag salamander

Chemical communication appears to be the primary mode of information transfer among woodland salamanders of the genus Plethodon. These pheromones have been shown to convey a wide variety of information, and are used to establish territorial boundaries. Responses to pheromones can include changes in both behavior and energy consumption. The ability of Ozark zigzag salamanders, Plethodon angusticlavius, to detect the sex of a conspecific through chemical substrate markings has not been assessed. In addition, some populations of P. angusticlavius are naturally infected with the ectoparasitic mite Hannemania eltoni. Parasitism can impact both the behavior and metabolism of the host as well as the behavior of others towards the parasitized individual. We examined whether sex and parasitism of the pheromone donor and receiver influenced behavioral and metabolic responses in P. angusticlavius. In laboratory trials, behavior of P. angusticlavius was influenced by the sex and parasite load of the pheromone donors and receivers. Behavioral responses were also influenced by whether the receiver was in its own territory or in the territory of the pheromone donor. Metabolic responses were not affected by the sex or parasite load of the pheromone donor, but males had higher metabolic rates than females, even after adjusting for differences in body size. Overall, our study indicates that both sex and parasitism can be detected via pheromones in this species, and the nature of the response depends on residency status, sex, and parasite load of the receiver.     

Female adaptation to developmental plasticity in male calling behavior

The katydid Neoconocephalus triops exhibits in North America substantial developmental plasticity of male mating calls. The AM rate of the summer calls is significantly faster than that of the winter calls at the same temperature. In the tropics, where N. triops originated, males express only the fast summer-call phenotype. We tested two alternative hypotheses: (1) call plasticity in the population from North America evolved in response to selection by female preference after N. triops colonized North America, or (2) call plasticity evolved before N. triops expanded into North America and its expression in the novel environment led to adaptive change of female preferences. First, we tested whether call plasticity was present in tropical populations of N. triops. Tropical males expressed the winter-call phenotype when reared under winter conditions, indicating that call plasticity did not evolve in response to temperate climates. Second, we compared female preferences among temperate and tropical populations. We found that the temperature dependence of preferred AM rate was significantly steeper in temperate N. triops than in a tropical population of N. triops. Third, we compared temperature dependence of female preference of the N. triops populations to three Neoconocephalus species without call plasticity. Only temperate N. triops had significantly steeper temperature dependence than the other species. This steeper temperature dependence matched female preference to the fast summer call at high temperatures and to the slow winter call at low temperatures in temperate populations. These results support the hypothesis that female preference changed in N. triops in North America to compensate for the plasticity of male calls.     

Dominance and reproductive behavior of wild house mice in a seminatural environment correlated with T-locus genotype

Summary Behavior of mice whose T-locus genotype was either +/+ or +/t was observed in a seminatural environment. Heterozygous females were less likely to be dominant, less likely to go into behavioral estrus, and less likely to become pregnant than were +/+ females. The relative fitness of +/t as compared with +/+ females was 0.32:1.00. In contrast, +/t males produced 35% more young than did +/+ males. The higher fitness of +/t males was primarily due to a greater production of young on the part of +/t as compared with +/+ subordinate males. Little evidence was found under these naturalistic conditions, of non-random mating with respect to T-locus genotype. The indication of overdominance for +/t males makes it unlikely that heterozygote disadvantage will be sufficient to account for the frequency of t-alleles in natural populations.     

The role of male harassment on female fitness for the dengue vector mosquito Aedes aegypti

Sexual harassment studies in insects suggest that females can incur several kinds of costs from male harassment and mating. Here, we examined direct and indirect costs of male harassment on components of female fitness in the predominantly monandrous mosquito Aedes aegypti. To disentangle the costs of harassment versus the costs of mating, we held females at a low or high density with males whose claspers were modified to prevent insemination and compared these to females held with normal males and to those held with females or alone. A reduced longevity was observed when females were held under high-density conditions with males or females, regardless if male claspers had been modified. There was no consistent effect of harassment on female fecundity. Net reproductive rate (R ₀) was higher in females held at low density with normal males compared to females held with males in the other treatments, even though only a small number of females showed direct evidence of remating. Indirect costs and benefits that were not due to harassment alone were observed. Daughters of females held with normal males at high density had reduced longevity compared to daughters from females held without conspecifics. However, their fitness (R ₀) was higher compared to females in all other treatments. Overall, our results indicate that A. aegypti females do not suffer a fitness cost from harassment of males when kept at moderate densities, and they suggest the potential for benefits obtained from ejaculate components.     

Sex-specific temperature dependence of foraging and growth of intertidal snails

Predicting the biological impacts of climate change requires an understanding of how temperature alters organismal physiology and behavior. Given differences in reproductive physiology between sexes, increases in global temperature may be experienced differently by the males and females of a species. This study tested for sex-specific effects of increased air temperature on foraging, growth, and survival of an intertidal snail, Nucella ostrina (San Juan Island, Washington, 48–30′44″N, 123–08′43″W). Snails exhibited periodic peaks in foraging. Subjecting snails to elevated low tide air temperatures did not alter the timing or magnitude of this pattern. Despite similar temporal patterns in foraging, females foraged more than males, even when the risk of thermal stress was high. While males and females appear to have a similar body temperature threshold for optimal growth, females were more likely to cross that threshold resulting in a loss of body mass when exposed to daily increases in air temperature. These results suggest that the consequences of a warming climate in the short term may be different for males and females of N. ostrina, but also imply longer-term costs of reduced reproductive output, abundance, and distribution of this ubiquitous intertidal predator. Generally, this study points to the possible significance of sex-specific responses in an increasingly warm world.     

Body temperature and body size affect flight performance in a damselfly

Flight performance is undoubtedly an important factor for behavioral success in flying insects. Though it is well-known that the flight performance is influenced by body temperature and body size, the relative importance of these factors is not well-understood. We performed laboratory experiments using the male-polymorphic damselfly Mnais costalis with larger territorial males and smaller non-territorial males in a population. We analyzed the effects of body temperature and body size, measured as the thoracic temperature and left hind-wing length, respectively, on two indices of flight performance: maximum lifting force and size-corrected lifting force. The latter is an index of acceleration that is related to aerial agility. The results showed that higher body temperature produced both larger maximum lifting force and larger size-corrected lifting force. In contrast, while larger size produced a larger maximum lifting force, it produced a lower size-corrected lifting force. The results of field measurements showed that territorial males had variable thoracic temperatures depending on the insolation in their territories. In contrast, non-territorial males had less variable and generally higher thoracic temperatures than territorial males as they are mostly found in sunny spots. Until now, the influence of body temperature on behavioral performance has remained unclear although considerable studies have suggested such influence. We showed, here, for the first time, combined effects of body size and body temperature on flight performance. We also showed that body temperature was influenced by the mating strategies of a damselfly. These findings provide new insights into the cost and benefits of territorial behavior in ectothermic animals.     

Estimation of algal growth parameters from vertical primary production profiles

Phytoplankton maximum growth rate and the saturation light intensity, I_s, can be estimated from vertical profiles of primary production by non-linear least-squares estimation. Solution through the normal equations leads to formulae which are relatively simple and easy to implement. The computation of confidence contours is demonstrated, and the results are contrasted to the confidence limits on the parameters individually. These can be quite misleading due to model non-linearity and correlation between parameter estimation.The procedure has been applied to primary production data from Lake Balaton, a shallow lake in Hungary. The growth rate-temperature relation is analysed by separating the parameters into two groups characteristic for “warm” and “cold” water phytoplankton, respectively. A bell-shaped curve is found for “cold” water communities, with an optimum at about 7–9°C, whereas the “warm” water phytoplankton exhibits a strong exponential dependency in the temperature range of interest (up to 25°C). I_s also appears to be related to temperature except for the “cold” water group, where I_s is essentially constant. However, a roughly linear relation with considerably less scatter is obtained when I_s is plotted directly versus day-averaged solar radiation. This apparent fast adaptation is attributed to the extremely short turnover time in Lake Balaton. Maximum growth rates of 10–20 d^?1 have been found for temperatures between 20 and 25°C. These results and a critical appraisal of available literature suggest that the common notion of maximum growth rates being in the order of 1–3 d^?1 needs revision, at least for lakes with relatively high summer temperatures.     

Black spots and female association preferences in a sexual/asexual mating complex (Poecilia, Poeciliidae, Teleostei)

We investigated whether female association preferences for males are influenced by black spot disease (BSD), a parasite induced change of the host phenotype. We compared three different species of fish: a gynogenetic hybrid species, Poecilia formosa (amazon molly) and two sexual species (Poecilia latipinna and Poecilia mexicana), which were involved in the natural hybridisation leading to the amazon molly. Contrary to their sexual relatives, asexual amazon mollies significantly avoided images of males infected with black spot disease. We propose that amazon molly females have direct fitness benefits from choosing healthy males. The adaptive significance of the preference for BSD-uninfected males in the asexual amazon molly is yet unclear but may involve avoidance of predation or parasite infection as well as increased sperm availability.     

Pre-copulatory sexual selection in the cigarette beetle Lasioderma serricorne

Traditional concepts of sexual selection and sexual conflict make different predictions about the costs and benefits to females of exposure to males with higher mating success. The traditional concepts of sexual selection assume that females benefit from their mate choices, whereas sexual conflict assumes that the females suffer greater costs by mating with males who have greater mating success and thus reduce their fitness. In order to understand how mate choice evolves, it is necessary to estimate the overall effect of mate choice on female fitness. However, relatively few studies have conducted that investigation. In this study, we investigated the direct and indirect effects of mating with attractive males on the fitness of females in the cigarette beetle Lasioderma serricorne. Mating with attractive males increased the number of female offspring but did not affect female longevity. Additionally, we found evidence that attractive males sire highly attractive sons. Thus, mating with an attractive male provides direct and indirect benefits but no fitness cost to female L. serricorne.     

Dissecting the mechanisms underlying old male mating advantage in a butterfly

Selection is expected to maximize an individual’s own genetic reward regardless of the potential fitness consequences for its sexual partners, which may cause sexual conflict. Although performance in holometabolous insects typically diminishes with age, old male mating advantage has been documented in a few species. Whether this pattern arises from female preference for older males based on, e.g., pheromone blends (intersexual selection), or from increased eagerness to mate in older compared to younger males is currently debated. We explore the mechanistic basis of old male mating advantage, using a series of experiments including behavioral as well as manipulative approaches, in the tropical butterfly Bicyclus anynana. Consistent with the residual reproductive value hypothesis, old male mating advantage was associated with a greater eagerness to mate, evidenced by a two times higher flying and courting activity in older than in younger males. In contrast, we found only limited support for a contribution of female preference for older males based on pheromone composition, although male sex pheromones clearly do play a role in mating success. Our results suggest that male behavior may play a primary role in old male mating advantage, and that pheromones are likely of secondary importance only. Male mating success was related to higher overall pheromone titers rather than variation in a single component. A dominant importance of male behavior in determining mating success may result in sexual conflict.     

Colony take-over and brood survival in temporary social parasites of the ant genus Formica

Parasites reduce host fitness, and so instigate counter adaptations by their hosts. In temporary social parasitism, usurpers must not only enter the colony unharmed, but also have their eggs reared by the host workers. We introduced parasitic Formica lugubris and Formica aquilonia queens into queen right and orphaned fragments of three host species, Formica cinerea, Formica picea and Formica fusca, and show that workers of all three host species kill over 40 % of the introduced queens within 10 days, regardless of the presence/absence of a resident queen, and parasite species. More parasite queens died in F. cinerea than in F. picea and F. fusca. There were no major differences in survival between the parasite species (except that F. lugubris survived longer than F. aquilonia in F. fusca colonies compared to F. picea colonies), but parasite queens survived longer in orphaned than in queen right fragments of F. fusca. Experimental introduction of parasite (F. aquilonia) eggs into orphaned colonies of F. fusca showed that none of the parasite eggs were reared until pupation; whereas on average, 12 % of the con-specific hetero-colonial eggs introduced in the same manner were reared until pupation. In all colonies that received parasite brood, all offspring consisted of worker-laid males, whereas the corresponding value was 50 % for colonies that received con-specific hetero-colonial brood. Thus, when the risks of entering host colonies and brood failure are combined, the rate of successful colony take-over is very low. Moreover, the host workers can to some extent alleviate the costs of parasitism by producing a final batch of own offspring.     

The effect of perceived female parasite load on post-copulatory male choice in a sex-role-reversed pipefish

The last several decades of research in behavioral ecology have resulted in a deeper appreciation of post-mating processes and sexual conflict in sexual selection. One of the most controversial aspects of sexual selection is cryptic mate choice. Here, we take advantage of male pregnancy in a sex-role-reversed pipefish (Syngnathus typhle) to quantify cryptic choice based on perceived parasite load and other sources of variance in female fitness. Studies have shown that S. typhle males preferentially mate with females with lower parasite loads and that a male’s perception of female parasite load can be altered by tattooing females. We manipulated the apparent parasite load of females in controlled mating experiments to test the hypothesis that post-copulatory sexual selection is dependent on a male’s perception of female parasite load in pipefish. Our results provided no evidence for cryptic male choice based on perceived female parasite load. However, we found evidence that eggs from larger females were more likely to result in viable offspring than eggs from smaller females and that the first female to mate with a male transferred more eggs per copulation on average. Overall, our results show that potential for post-copulatory sexual selection does exist in pipefish, but the male’s perception of female parasite load does not play a major role in this process.     

Bacterial diversity of the sediments transiting through the gut of Holothuria scabra (Holothuroidea; Echinodermata)

This work analyzes bacterial diversity of sediments transiting through the gut of Holothuria scabra which is an important bioturbator in tropical shallow waters. This edible holothurian species has a social and economic importance for coastal populations in many developing countries. Bacterial biodiversity was analyzed by sequencing the 16S rRNA of bacterial cultures and clones. DAPI and FISH methods were used to determine and compare the number of bacteria found in the various gut compartments. A total of 116 phylotypes belonging to the γ-Proteobacteria (60.5 %), α-Proteobacteria (24.5 %), Bacteroidetes (6 %), Actinobacteria (2.75 %), Fusobacteria (1.75 %), Firmicutes (1.75 %), Cyanobacteria (1.75 %) and δ-Proteobacteria (1 %) were identified. The number of bacteria is significantly greater (1.5×) in the foregut than in the ambient sediments. The number of bacteria significantly decreases in the midgut and remains stable until defecation. Some γ-Proteobacteria, especially Vibrio, are less affected by digestion than other bacterial taxa. The season has an impact on the bacterial diversity found in the sediments transiting through the gut: in the dry season, γ-Proteobacteria are the most abundant taxon, while α-Proteobacteria dominate in the rainy season. Vibrio is the most frequent genus with some well-known opportunistic pathogens like V. harveyi, V. alginolyticus and V. proteolyticus. Findings show that sediment-associated microbial communities are significantly modified by H. scabra during their transit through the gut which supports the view that holothurians play a substantial role in the structuring of bacterial communities at the sediment–seawater interface.     

Higher calcification costs at lower temperatures do not break the temperature-size rule in an intertidal gastropod with determinate growth

The vast majority of ectothermic organisms grow larger when developing at cooler environmental temperatures, a pattern frequently referred to as the temperature-size rule (TSR). Assuming that this reaction norm has adaptive significance, life history theory predicts that converse patterns may evolve if favored by natural selection, namely if the costs associated with complying to the TSR outweigh the benefits. Calcifying ectotherms may comprise such an exception not following the TSR, because calcification is expected to be more costly at lower temperatures thus increasing associated costs. To test this hypothesis, we reared wild-caught juveniles of the intertidal gastropod Monetaria annulus and compared their shell sizes at the end of the juvenile stage between two rearing temperatures. Contrary to our prediction, M. annulus does follow the TSR, suggesting that increased calcification costs at lower temperatures are not high enough to break the TSR. Such plastic responses should be considered when interpreting geographical patterns such as latitudinal size clines, which may be caused at least partly by phenotypic plasticity.     

Growth dynamics of the seagrass <Emphasis Type="Italic">Halophila nipponica</Emphasis>, recently discovered in temperate coastal waters of the Korean peninsula

Seagrass species in the genus Halophila are usually distributed in tropical or subtropical areas, but a Halophila species identified as H. nipponica was first observed in temperate coastal regions of Korea in 2007. Since this species mainly occurs in warm temperate regions influenced by warm currents, we hypothesized that H. nipponica may exhibit different growth patterns from those of other temperate seagrass species in Korea, instead showing similar growth dynamics to tropical/subtropical species. The growth and morphology of H. nipponica in relation to coincident measurements of environmental factors were investigated from July 2008 to September 2009 to examine the growth dynamics of this species. Water temperature at the study site ranged from 9.7°C in January to 25.1°C in August. Shoot density, biomass, and productivity exhibited significant seasonal variation, increasing during summer and decreasing during winter. Productivity was severely restricted to nearly ceasing at water temperatures less than 15°C, and winter minimum growth lasted until May. The optimal temperature for H. nipponica growth was approximately 25°C, which was the maximum water temperature at the study site, and no growth reduction in high summer water temperature was observed. Thus, H. nipponica on the temperate coast of Korea exhibited a distinctly different growth pattern from those of temperate seagrass species in Korea, which have shown great reductions in growth at water temperatures higher than 20°C. Higher below- to above-ground ratio and leaf burial into sediments with shorter leaf petioles during winter might be overwintering strategies in this species. The growth patterns of H. nipponica at the study site imply that this species still possess the tropical characteristics of the genus Halophila.     

Do brain parasites alter host personality? — Experimental study in minnows

Despite that the existence of animal personalities is widely recognized, no consensus has been reached on the relative importance of different ecological factors behind their expression. Recently, it has been suggested that parasites may have a crucial role in shaping animal personalities, but only a very few studies have experimentally tested the idea. We infected Eurasian minnows (Phoxinus phoxinus) with the brain-encysted trematode parasite, Diplostomum phoxini, and studied whether infection could modify the personality of their hosts. Our results show that D. phoxini infection did not affect the mean levels of boldness, activity or exploration, but infected minnows showed higher repeatability in boldness and activity, and reduced repeatability in exploration. We also found that D. phoxini may be able to break the associations (behavioral syndromes) between behavioral traits, but that this effect may be dependent on parasite intensity. Furthermore, the effect of D. phoxini infection on personality of the hosts was found to be nonlinearly dependent on infection intensity. Taken together, our results suggest that D. phoxini parasites may shape the personality of their hosts, but that behavioral consequences of ecologically relevant infection levels may be rather subtle and easily remain undetected if only the mean trait expressions are compared.     

Breeding success of a brood parasite is associated with social mating status of its host

Reproductive success of brood parasites varies considerably both among and within host species, mainly due to differences in host egg-rejection rates and survival of parasitic chicks. Here, we investigated the breeding success of the cuckoo (Cuculus canorus) in one of its major hosts, the great reed warbler (Acrocephalus arundinaceus), with respect to host social mating status. In this passerine, polygynous males provide less parental care to their young per nest than monogamous males. Consequently, their less-assisted females may fledge lower numbers of nestlings than monogamous females. This may be especially true for secondary females, which often receive limited or no paternal help with young at all. Based on these findings, we expected higher cuckoo reproductive success in nests of socially monogamous than polygynous great reed warbler males. More specifically, we predicted lower fledging success of cuckoo young in nests of secondary than primary or monogamous females. In line with the prediction, we found higher cuckoo fledging success in nests of monogamous than polygynous males, monogamous nests being more than twice as successful as secondary nests. We detected, however, only a tendency to lower cuckoo success in primary compared to monogamous nests and no differences between primary and secondary nests. Moreover, neither parasitism nor host egg-rejection rates differed among the nests of different status. Our results show, for the first time, that the social mating status of a host may influence the overall reproductive success of a brood parasite and thus should be considered in further studies.     

The stress of scramble: sex differences in behavior and physiological stress response in a time-constrained mating system

Iteroparous species maximize lifetime reproductive fitness by balancing current and future reproductive investments. In order to maximize fitness in the face of social or environmental heterogeneity, individuals of the same species may vary in whether they prioritize current reproductive opportunity or sacrifice immediate reproduction in order to prioritize survival and future reproductive potential. Glucocorticoid (GC) secretion plays an important role in mediating this trade-off by promoting behavioral and physiological responses associated with survival, often at the expense of nonessential (e.g., reproductive) functions. We used wood frogs (Lithobates sylvaticus [Rana sylvatica]) to test whether males and females differed in their (a) physiological response (plasma corticosterone [CORT] concentration) to standardized handling stress—a proxy for predation threat—and (b) performance of reproductive behaviors that may enhance their conspicuousness to predators. We also tested whether levels of male competition influenced sex differences in these factors, as more intense competition may require males to devote more time to risky reproductive behaviors. We found that females had lower baseline CORT but exhibited a significantly greater CORT response to a stressor and spent less time performing potentially risky behavior (surface floating) than did males. These sex differences were consistent across different levels of male mating competition. Our results reveal that during breeding, males and females may differentially respond to stressors and perform risk-prone behaviors, despite facing the same extreme breeding constraints, providing new insight into the survival-reproduction trade-off of explosively breeding species.