Environmental degradation of the Obra-Renukoot-Singrauli Area,India, and its impact on natural and derived ecosystems

Summary Vast stretches of the Obra—Renukoot—Singrauli region were once covered with natural forests. The tribal dwellings were small and interspersed. However, there has been rapid industrialisation in recent years. Quarrying for limestone, the establishment of a cement factory, thermal power stations and the construction of the G.B. Pant Sagar reservoir have resulted in a rapid build up of human population, the displacement of the original population, deforestation and conversion of natural forest ecosystems into savanna and marginal croplands. The converted ecosystems are under immense biotic stresses such as lopping, grazing, etc.The rainfall is meagre and erratic, the soils are highly weathered and impoverished, consequently the natural forests, as well as the derived ecosystems, are fragile. Signs of desertification are widespread. A rapid depletion in the wildlife has occurred. The establishment of thermal power stations and chemical and cement factories has also resulted in large scale gaseous air pollution, particularly of SO₂ and HF, pollution due to particulate matter through fly ash and cement dust, and that due to liquid effluents. Surface coal mining has caused extensive damage to the natural ecosystems with growing dumps of overburden. The latter needs to be stabilised.This integrated research project on environmental degradation and its impact on air, soil, water and vegetation was divided into five sub-projects, each supervised by a principal investigator. These sub-projects were: (i) Industrial air pollutants and their effects on ecosystem structure and function; (ii) Analysis of structure and functioning of forest ecosystems; (iii) Analysis of structure and functioning of savanna and agroecosystems; (iv) Analysis of structure and functioning of watershed and water-bodies; and (v) Impact of land-use system, socio-economic- demographic conditions, and environmental perception and behavioural management. In these studies dynamics of plant biomass, productivity and nutrients, and biochemical—physiological responses of plants to pollution were emphasised. Environmental perceptions of the native and displaced populations were also studied.The paper outlines a range of recommendations which should help to improve the environmental quality of the region.This article summarises a Man and the Biosphere research project which was sponsored by the Ministry of Environment and Forests of the Government of India. The team of principal investigators comprised Professors J.S. Singh, D.N. Rao, R.S. Ambasht, K.P. Singh, B.S. Gupta and M. Agrawal. The study was coordinated by Professor J.S. Singh, Professor of Botany at Banaras Hindu University.     

Differential fitness of allelic isozymes in the marine gastropodsLittorina punctata andLittorina neritoides,exposed to the environmental stress of the combined effects of cadmium and mercury pollution

The present study tested the separate and the interactive pollution effects of cadmium and mercury on the electrophoretically detected allelic isozyme frequencies of the enzyme phosphoglucose isomerase for two species of littoral marine gastropods —Littorina punctata andL. neritoides — and the enzyme amino peptidase forL. neritoides. Our results indicate differential survivorship of allelic isozyme genotypes specific for each type of pollutant and for their interaction, as well as trends common to all pollutants. Theoretically the results reflect the adaptive nature of at least some allozymic genotypes in these marine gastropods and seem inconsistent with the neutral theory of allozyme polymorphisms. Practically, the results reinforce earlier conclusions that changes in the frequency of allelic isozymes may be used as a genetic monitor of pollution.     

The impact of some specific air pollutants on agricultural productivity

Summary Unplanned discharges of atmospheric pollutants influenced by intense industrialisation, the population explosion and urbanisation result in injury and damage. The intensity and nature of the damage is a function of the concentration of the pollutant and the duration of exposure (dose). This has immediate (acute) and long (chronic) effects on the quantity and quality of agricultural products.Based on the properties of these pollutants, the resulting effects on the receptor may cause biochemical and physiological modifications at the cellular level as well as whole plant, animal and ecosystem alterations. These may cause significant changes in agricultural output.Mr E.M. Shaibu—Imodagbe is Vice—Principal and a lecturer at the Samaru College of Agriculture, of Ahmadu Bello University. He holds a Master's degree in Pollution and Environmental Control from the University of Manchester, UK, and his research special interests are in pollution damage and environmental quality.     

Emergent aspects of environment: A creative challenge

Summary Our environmental situation continues to decline faster than ever. This is demonstrated by, the growing spread of pollution of both the water and atmospheric systems, erosion of topsoil, tropical deforestation, desertification, mass extinction of species, depletion of the ozone layer, and climatic dislocations. Compounding the impact of these problems is growth in human numbers in developing countries, together with the associated factor of extensive poverty. The author asks why our environmental situation should continue to decline, despite a remarkable outburst of awareness on the part of the general public and its political leaders since the time of the Stockholm Conference on the Human Environment. The author concludes that the problem does not lie with inadequate effort, rather with misdirected effort: we persist in tackling the symptoms of problems, rather than the problems themselves, let alone the sources of the problems. The paper broaches these issues within a context of fast-growing interdependence among the community of nations — both economic and ecological interdependence — which presents yet more problems, but also raises many opportunities for creative action.Professor Norman Myers is a consultant in environment and development. He has worked since 1970 on the general subject area of environment and natural resources, with emphasis on species, gene reservoirs and tropical forests. He was undertaken this consultancy work for the Rockefeller Brothers Fund, US National Academy of Sciences, the World Bank, OECD, the Smithsonian Institution, UN agencies, World Resources Institute, US National Research Council and IUCN, among other organizations. His main professional interest lies with resource relationships between the developed and developing worlds.Among his recent publications areThe Sinking Ark (Pergamon Press, Oxford and New York, 1979);Conversion of Tropical Moist Forests (National Research Council, Washington D.C., 1980);A Wealth of Wild Species (Westview Press, Boulder, Colorado, 1983);The Primary Source (W. W. Norton, New York, 1984); andThe Gaia Atlas of Planet Management (Doubleday, New York, and Pan Books, London, 1985).Norman Myers is an Advisory Board Member and regular contributor toThe Environmentalist.     

Choosing restorative environments across the lifespan: A matter of place experience

Past research on restorativeness has emphasized mainly the potential of natural environments. In our hypothesis, built environments are also likely to be recognized as restorative places. In this study, focusing on restorative experiences more than on environments alone, attention is drawn on the relative importance of the four restorative components proposed by “attention restoration theory”—being-away, extent, fascination, compatibility—in leisure experiences of people at different stages of the lifespan, and on the characterization of these experiences in terms of relaxation and excitement. We also take account of the time available for restoration and the context in which the need for restoration may emerge, according to three models of the relationships between work and leisure: spill-over, compensation and segmentation. Results show that natural and built environments can have different restorative potentials in relation to the stage of the lifespan and to the time available for restoration; moreover, in people's perception, the four restorative components differ from each other in their relative importance. The social and affective dimensions came out as important features of restorative experiences. Finally, relaxation and excitement in leisure patterns were shown to be differentially related to work characteristics. Briefly, restorativeness emerged as the result of a global “place experience”.     

An ecological analysis of predominant land uses in the Brazilian Amazon

Summary The land uses that now predominate in Brazil's Amazon Region are unlikely to produce sustainable yields. They also tend to close off potentially sustainable alternative uses. Cattle pasture — either productive or abandoned — now occupies most deforested land. Small farmers plant pasture after using the land for a year or two under annual crops, while large cattle ranches plant pasture directly after clearing. The principal motive for planting pasture is often its low cost and high effectiveness as a means of securing speculative land claims — not beef production.Pasture and cattle yields are low and, after use for about a decade, the planted grasses are out-competed by secondary forest species or inedible grasses. Depletion of available phosphorus in the soil is a major cause of yield decline; Brazil's relatively modest phosphorus deposits, virtually all of which are outside of Amazonia, make fertiliser use not feasible for the vast areas now rapidly being converted to pasture. Converting a substantial portion of Amazonia to pasture would have potential climatic effects. Areas that can be planted in annual and perennial crops are restrained by world markets, as well as by soil quality and Brazil's limited stocks of the inputs needed for intensive agriculture.Recent initiatives for agricultural-ecological in Brazil's Amaoznian states could be a first step toward more rational land use. Immediate measures are needed to slow deforestation, to discourage unsustainable uses and to make sustainable alternatives profitable.Professor Philip Fearnside is currently Research Professor in the Department of Ecology at the National Institute for Research in the Amazon. The paper has been modified from an earlier version presented at the International Symposium on Alternatives to Deforestation held in Belém, Pará, Brazil in January 1988.     

Michoacán centre for science and technology education a summary of the project

Summary This paper looks at the problems of science and technology education in Mexico, and proposes ways in which the quality of education at every level can be improved. It advocates an interdisciplinary approach, and from that approach to science education, the development of environmental education.Prof. Gutiérrez-Vázquez received his training in microbiology and biochemistry in the National Polytechnic Institute in his native Mexico City, receiving his degree in 1954. He went from instructor to assistant professor (1950–1960), becoming a full professor, deputy dean and then dean of the Faculty of Biology in the same Institute (1961–1969); he published two books and some 20 papers dealing with the physiology of tubercle bacillus and other mycobacteria, as well as with other topics on microbiology. More recently (1970 to date), he became a full professor in the Center for Advanced Studies of the same Institute, where he has been head of the Department of Educational Research (1972–1981) and carried out research and development projects on science education for children and adolescents. During this period he has published some 20 more books and 30 odd papers, and written more than 80 science programmes for commercial television.     

Acid surface waters: the prognosis for fish

Summary The extent of surface water acidification in Northern Europe and in Britain is reviewed and information is given on the degree to which changes in water quality appear to have influenced fish populations. The relationship between water quality and fish has been clarified by laboratory exposures, demonstrating that levels of calcium and aluminium, as well as acidity, are important for fish. It is not easy, however, to transpose laboratory findings to the field. Other determinants of a fishery should not be ignored — they include morphological and physical characteristics of lakes and streams, and biological characteristics such as food supply and species interactions.The recovery or restoration of fisheries in acid waters is crucially dependent on replacement of calcium and reduction of aluminium to bring the water quality into a range suitable for fish. While reduction of sulphur emissions, or of the atmospheric deposition of all acid generating materials, may be expected to have some effect on lake sulphate concentrations, the degree to which acidity might be realistically reduced is quite uncertain.Dr. G. D. Howells was, until recently, Head of Biology at the Central Electricity Research Laboratories of the United Kingdom's Central Electricity Generating Board (CEGB). Her previous experience includes her first degree, obtained in New Zealand, a subsequent research degree from the University of Cambridge, and three years at the University of New York studying the effects of power station siting on the Hudson River. She has a wide experience of studying pollution problems. This paper was presented at the Institution of Environmental Sciences Conference,An Update on Acid Rain, November 1984, and is published by permission of the Central Electricity Generating Board.     

The biodiversity crisis and the future of evolution

A large proportion of existing species — possibly half, conceivably even more — may be lost within the foreseeable future. But this may not prove to be the most consequential outcome of the current biodiversity crisis. More significant could be the disruption and degradation of several basic processes of evolution. It appears likely that for mass extinction episodes (MEEs) in the geological past, the recovery period usually lasted at least five million years. Because of certain unique features of the present MEE — notably the near elimination of biomes such as tropical forests, wetlands and coral reefs, which have served as powerhouses of evolution in the past — the bounce-back phase could extend several times longer than five million years. Among distinctive features of future evolution could be; in the short term, homogenization of biotas, a proliferation of opportunistic species, an outburst of speciation among particular taxa, and a pest-and-weed ecology; and, in the long term, a decline of biodisparity, the elimination of megavertebrates, an end to speciation among large vertebrates, and multiple constraints on origination, innovation and adaptive radiation. These disruptive phenomena would rank among the most prominent departures in the entire course of evolution. Full knowledge and understanding of what may characterize future evolution remains largely a black hole of research. As a consequence, conservation policies fail to reflect a further problem of the biodiversity prospect, perhaps exceeding the better recognized problem of the mass extinction of species.Professor Norman Myers is an Editorial Board member and regular contributor toThe Environmentalist. He is an Honorary Visiting Fellow at Green College, Oxford. This paper is a greatly expanded version of a preliminary probing in a popular magazine a decade ago (Myers, 1985). It has been prompted by a major international conference organized by the US National Academy of Sciences, scheduled for late 1996.     

Phenomenology and the clark experience

The author argues that his research and teaching interests are very much a legacy from Clark University, especially because of events there during the years 1970–72. The essay first identifies four factors—place, time, people, and atmosphere—which seem to have shaped developments in environment-behavior research during that time at Clark and considers how those developments touched the author personally. Second, the author reviews his present research interests in terms of three themes: fostering careful looking and seeing; examining environmental experience; and establishing a phenomenology of place and place-making. He argues that each of these themes is in part a product of his Clark experience during the early 1970s.     

The risks of radiation

Summary The risks of radioactivity are a really complicated matter, yet they are much better known than are the risks relating to thousands of chemical poisons that occur in our environment. The greatest mistakes are probably made in the definition of safety margins. Except for the bombs dropped in Japan and one other case in the Marshall Islands, there has always—luckily—been a wide safety margin between fallout radiation and doses dangerous to health; the margin has actually been about 1000-fold. The Chernobyl dose of 0.5 mGy/year that we received is only 1/1000 of the acute dose of 0.5 Gy which would cause a slight and nonpermanent change in the blood picture.There is no such safety margin with respect to many air pollutants. The safety standards for sulfuric or nitric oxides, ozone and so on, have been set only just below the level that already causes a health hazard, and these standards are exceeded once in a while. Otherwise, traffic would have to be forbidden and many industrial plants, especially power stations using coal, would have to stop working whenever a low-temperature inversion occurred. Environmental radioactivity does not represent a likely health risk in Finland unless a nuclear war breaks out. Air pollutants, on the contrary, are a real and almost daily health risk that should be carefully considered when decisions about our energy production are being made.In spite of what happened at Chernobyl, global consumption of nuclear power will double by the year 2000, since there are about 140 nuclear power plants presently under construction. It is not likely that another catastrophe like Chernobyl will happen, yet nuclear plant accidents are of course possible, even if their likelihood is diminished by improving reactor safety and even if any eventual damage could be expected to be smaller. If a reactor is hooded by a containment structure, no significant release of radioactive materials should be possible even in case of an accident. However, we must be prepared to protect the population with carefully planned civil defense and radiation protection measures.I would like to sum up all of this as follows: The risks of radiation are a fact, but the fear they arouse is often disproportionate. There are risks related to all other methods of energy production as well; nuclear power is no exception in that respect. It is good that we aim at greater safety, but I hope that false presumptions will not lead us to mistaking a less safe alternative for a safer one.     

Water/acid gas interfacial tensions and their impact on acid gas geological storage

Acid gas geological disposal is a promising process to reduce CO₂ atmospheric emissions and an environment-friendly and economic alternative to the transformation of H₂S into sulphur by the Claus process. Acid gas confinement in geological formations is to a large extent controlled by the capillary properties of the water/acid–gas/caprock system, because a significant fraction of the injected gas rises buoyantly and accumulates beneath the caprock. These properties include the water/acid gas interfacial tension (IFT), to which the so-called capillary entry pressure of the gas in the water-saturated caprock is proportional. In this paper we present the first ever systematic water/acid gas IFT measurements carried out by the pendant drop technique under geological storage conditions. We performed IFT measurements for water/H₂S systems over a large range of pressure (up to P = 15 MPa) and temperature (up to T = 120 °C). Water/H₂S IFT decreases with increasing P and levels off at around 9–10 mN/m at high T (≥70 °C) and P (>12 MPa). The latter values are around 30–40% of water/CO₂ IFTs, and around 20% of water/CH₄ IFTs at similar T and P conditions. The IFT between water and a CO₂ + H₂S mixture at T = 77 °C and P > 7.5 MPa is observed to be approximately equal to the molar average IFT of the water/CO₂ and water/H₂S binary mixtures. Thus, when the H₂S content in the stored acid gas increases the capillary entry pressure decreases, together with the maximum height of acid gas column and potential storage capacity of a given geological formation. Hence, considerable attention should be exercised when refilling with a H₂S-rich acid gas a depleted gas reservoir, or a depleted oil reservoir with a gas cap: in the case of hydrocarbon reservoirs that were initially (i.e., at the time of their discovery) close to capillary leakage, acid gas leakage through the caprock will inevitably occur if the refilling pressure approaches the initial reservoir pressure.     

Methane fermentation of dung from litter-free reared domestic animals — an optimisation study

Data on the quantity (27 453 tons from litter-free reared animals in Bulgaria, only) and the chemical and energy characteristics of dung produced in intensive management farms for domestic animals suggests that technologies combining biogenic elements recycling with energy utilistation and dung decontamination are expedient to be applied on these types of farms. To this effect a fermenter was designed and a mathematical model (a Chen-Hashimoto model based computer programme) was applied, as a result of which the optimum methane fermentation parameters were determined. The technological methane output (Y_v) — indicator of biogas production efficiency (output/dm³ fermentor volume) showed an optimum at temperature 55°C and period of exchange 6 days. The methane output per unit mineralised organic matter in the substrate (B) — assumed as an indicator of ecological efficiency (maximum organic matter degradation) exhibited an optimum at 33°C for 15 days period of exchange.     

Tropical forests: The policy challenge

Summary Tropical forests are being destroyed faster than ever, the rate having almost doubled during the 1980s. The main agent of deforestation now turns out to be the displaced peasant or landless farmer, sometimes known as the shifted cultivator; that group accounts for more forest loss than the combined impacts of the commercial logger, the cattle rancher and all other better known sources of deforestation. Yet, the over-riding target of conservation efforts remains the minor players in the forest's decline. All too little attention is directed towards the shifted cultivator, and next to nothing is done to address the problem which this group represents. Policy purviews need to be expanded to include the key factor of the shifted cultivator. In turn, it must determined what impels these people to abandon established farming areas in the countries concerned and to migrate into the forests. It appears that these people are driven by an array of forces: population growth, maldistribution of arable lands, inadequate rural infrastructure and lack of government attention to subsistence agriculture. In other words, the source problem is an amalgam of non-forestry factors, and conservationists will not achieve success in safeguarding the remaining forests unless they direct much more emphasis towards these root causes of deforestation.Dr Norman Myers is a consultant in environment and development, a member of this journal's Advisory Board and a regular contributor to the journal (seeThe Environmentalist,8(3), 187–208 and10(4), 243–256).     

Managing a Subsidized Predator Population: Reducing Common Raven Predation on Desert Tortoises

Human communities often are an inadvertent source of food, water, and other resources to native species of wildlife. Because these resources are more stable and predictable than those in a natural environment, animals that subsist on them are able to increase in numbers and expand their range, much to the detriment of their competitors and species they prey upon. In the Mojave Desert, common ravens (Corvus corax) have benefited from human-provided resources to increase in population size precipitously in recent years. This trend has caused concern because ravens prey on juvenile desert tortoises (Gopherus agassizii), a federally threatened species. In this paper, I discuss management strategies to reduce raven predation on desert tortoises. The recommendations fall into three categories: (1) managing raven populations by reducing access to anthropogenic resources; (2) removing offending ravens or other birds in specially targeted tortoise management zones; and (3) continuing research on raven ecology, raven behavior, and methods of reducing raven predation on tortoises. I also recommend approaching the problem within an adaptive management framework: management efforts should first be employed as scientific experiments—with replicates and controls—to yield an unbiased assessment of their effectiveness. Furthermore, these strategies should be implemented in concert with actions that reduce other causes of desert tortoise mortality to aid the long-term recovery of their populations. Overall, the approaches outlined in this paper are widely applicable to the management of subsidized predators, particularly where they present a threat to a declining species of prey.     

The biodiversity challenge: Expanded hot-spots analysis

Summary This paper aims to throw light on the mass extinction that is overtaking Earth's species. Using an analytic methodology developed for an earlier partial assessment, it focuses on a series of hot-spot areas, these being areas that (a) feature exceptional concentrations of species with high levels of endemism, and (b) face exceptional threats of destruction. The paper identifies another eight such areas, four of them in tropical forests and four in Mediterranean-type zones. The analysis reveals that the four tropical-forest areas contain at least 2,835 endemic plant species in 18,700 km², or 1.1 percent of Earth's plant species in 0.013 percent of Earth's land surface; and that the four Mediterranean-type areas contain 12,720 endemic plan: species in 435,700 km², or 5.1 percent of Earth's plant species in 0.3 percent of the Earth's land surface. Taken together, these eight hot-spot areas contain 15,555 endemic plant species in 454,400 km², or 6.2 percent of Earth's plant species in 0.3 percent of Earth's land surface. This is to be compared with the earlier hot-spots analysis of 10 tropical-forest areas, with 34,400 endemic plant species in 292,000 km², or 13.8 percent of Earth's plant species in 0.2 percent of Earth's land surface.Taking all 18 hot-spot areas together, we find they support 49,955 endemic plant species, or 20 percent of Earth's plant species, in 746,400 km², or 0.5 percent of Earth's land surface. This means that one fifth of Earth's plant species are confined to half of one percent of the Earth's land surface — and they occur in habitats that are mostly threatened with imminent destruction.By concentrating on these hot-spot areas where needs are greatest and where the pay-off from safeguard measures would be greatest, conservationists can engage in a more systematised response to the challenge of large scale impending extinctions.Dr Norman Myers is a consultant in environment and development, a member of this journal's Advisory Board, and a regular contributor to the journal. He is a Senior Fellow of World Wildlife Fund — US. This paper enlarges on an important theme developed by Dr Myers in a contribution which appeared inThe Environmentalist,8(3), 187–208. The research for this paper has been supported by the MacArthur Foundation, Chicago, USA.     

An exploration of place as a process: The case of Jackson Hole, WY

A variety of theoretical positions and frameworks have been advanced to account for how places become “places”—in other words, how places become meaningful. Most existing frameworks share the idea that a place is a complex concept, given life by people attaching meaning to a physical setting in a variety of ways. This paper explores how places evolve as ever-shifting points of meaning that mark changes in people's lives in response to a variety of influences. Data collected from in-depth personal interviews in and around Jackson, Wyoming, are used to explore the conceptualization of place as a process, rather than a static entity. Three key themes or dimensions that were frequently described by interviewees are examined: (1) life stage/course, (2) searching for a feeling, and (3) commitment to a place. Not only did some respondents describe what made places important to them (the components, such as social ties, or favorite activities), they also described how these attributes worked together to create and maintain place meanings over the course of their lives. During the interviews, it was noted that these dimensions and processes were expressed explicitly as well as implicitly.     

Ecological effects of the frog's legs trade

Summary The author reviews the trade in frogs from India, conducted to provide a culinary delicacy in frogs' legs for the West. Excessive harvesting of frogs upsets the ecological balance in their natural habitats and increases the need for extensive use of insecticides, with consequent additions to both pollution and costs.Dr G.M. Oza is Reader in Botany in the Faculty of Science at the Maharaja Sayajirao University of Baroda. He is also General Secretary of the International (formerly Indian) Society of Naturalists (INSONA), and Founding Editor ofEnvironmental Awareness. He serves as a Member of the Commission on Ecology and the Commission on Education and Training of IUCN — the World Conservation Union. This paper was first submitted for publication in 1986.     

Implications of Land Use Changes on Carbon Dynamics and Sequestration—Evaluation from Forestry Datasets, India

Forests and soils are a major sink of carbon, and land use changes can affect the magnitude of above ground and below ground carbon stores and the net flux of carbon between the land and the atmosphere. Studies on methods for examining the future consequences of changes in patterns of land use change and carbon flux gains importance, as they provide different options for CO₂ mitigation strategies. In this study, a simulation approach combining Markov chain processes and carbon pools for forests and soils has been implemented to study the carbon flows over a period of time. Markov chains have been computed by converting the land use change and forestry data of India from 1997 to 1999 into a matrix of conditional probabilities reflecting the changes from one class at time t to another class time t+1. Results from Markov modeling suggested Indian forests as a potential sink for 0.94 Gt carbon, with an increase in dense forest area of about 75.93 Mha and decrease of about 3.4 Mha and 5.0 Mha in open and scrub forests, if similar land use changes that occurred during 1997–1999 would continue. The limiting probabilities suggested 34.27 percent as dense forest, 6.90 as open forest, 0.4 percent mangrove forest, 0.1 percent scrub and 58 percent as non-forest area. Although Indian forests are found to be a potential carbon sink, analysis of results from transition probabilities for different years till 2050 suggests that, the forests will continue to be a source of about 20.59 MtC to the atmosphere. The implications of these results in the context of increasing anthropogenic pressure on open and scrub forests and their contribution to carbon source from land use change and forestry sector are discussed. Some of the mitigation aspects to reduce greenhouse gas emissions from land use change and forestry sector in India are also reviewed in the study.     

Continuous pollution monitoring using Photobacterium phosphoreum

Photobacterium phosphoreum is a marine bacterium which is used extensively as a bioluminescent indicator of pollutants, where the presence of toxicants diminishes light output. To evaluate the utility of cell immobilisation in continuous toxicity testing, the sensitivity of P. phosphoreum to five gelling agents was evaluated relative to the retention of bioluminescence in 3% NaCl-glycerol suspensions. Following storage at 4°C, the control cultures retained light output for up to 2 weeks before significant decline; alginate-glycerol suspensions were stable for up to 4 weeks and bioluminescence was detectable for up to 6 weeks. Cells stored in agar were no more stable than the control, whereas cells gelled in agarose and low-melting point agarose showed a significant decline in bioluminescence within 2 weeks of storage. Bioluminescence was totally retained in alginate-glycerol suspensions stored at −80°C for up to 12 weeks. P. phosphoreum was successfully immobilised in strontium alginate and showed a dose-related response to four of the five heavy metal ions, SDS and pentachlorophenol tested when responses were followed over a time-course. A flow-through system for Sr-alginate immobilised cells was developed and conditions for operation were optimised. When cells were exposed to a pulse of 4-nitrophenol or salicylate then the nutrient feed continued, bioluminescence declined in response (pulse of 4–6 min) to these pollutants then recovered to a new stable rate of decline which was faster than the pre-exposure rate. These results demonstrate the potential of using immobilised P. phosphoreum in a continuous flow-through system for real-time environmental monitoring of water quality.