Biomarkers of pesticide exposure and diabetes in the 1999–2004 National Health and Nutrition Examination Survey

The associations of 8 pesticides and pesticide metabolites with total diabetes (diagnosed and undiagnosed) and pre-diabetes (glycohemoglobin 5.7–6.4%) were evaluated using the National Health and Nutrition Examination Survey (NHANES), 1999–2004. Six of the pesticides were found to be associated with total diabetes in separate adjusted logistic regressions. These pesticides and pesticide metabolites were beta-hexachlorocyclohexane, p,p′-DDE, p,p′-DDT, oxychlordane, trans-nonachlor, and heptachlor epoxide. When the number of compounds elevated was tested, 4 or more, of the 6, elevated had an odds ratio of 4.99 (95% CI 1.97–12.61) compared to none elevated. When the 6 compounds were tested together in a single combined adjusted logistic regression only oxychlordane, a metabolite of chlordane, and heptachlor epoxide, a metabolite of heptachlor, were significantly associated with total diabetes. In the combined adjusted logistic regression, oxychlordane ≥ 14.5 ng/g lipid adjusted had an odds ratios of 1.90 (95% CI 1.09–3.32) compared to oxychlordane < 14.5 ng/g lipid adjusted, and heptachlor epoxide ≥ 14.6 ng/g lipid adjusted had an odds ratio of 1.70 (95% CI 1.16–2.49) compared to heptachlor epoxide < 14.6 ng/g lipid adjusted. Heptachlor epoxide and p,p′-DDT were significantly associated with pre-diabetes in separate adjusted logistic regressions. When these 2 compounds were tested together only heptachlor epoxide remained significantly associated with pre-diabetes. The evidence supporting the relationship between pesticides and pesticide metabolites, with diabetes, was strongest for heptachlor epoxide and oxychlordane, intermediate for p,p′-DDT, and least for beta-hexachlorocyclohexane, p,p′-DDE, and trans-nonachlor. Mirex and dieldrin were not associated with total diabetes or pre-diabetes.     

DDTs in mothers' milk,placenta and hair,and health risk assessment for infants at two coastal and inland cities in China

This study is a one of the very few investigating the dichloro-diphenyl-trichloroethanes (DDTs) (summation of o,p′-DDE, p,p′-DDE, o,p′-DDD, p,p′-DDD, o,p′-DDT, and p,p′-DDT) in multiple human matrices in mothers' milk, placenta and hair collected from residents from two coastal cities: Guiyu (GY) and Taizhou (TZ) and one inland city: Lin'an (LA). TZ (milk: 360 ± 319 ng/g lipid wt.) showed significantly higher concentrations of DDTs than those from LA (milk: 190 ± 131 ng/g lipid wt.), whereas, concentrations of DDTs in GY (milk: 305 ± 109 ng/g lipid wt.) were in between TZ and LA. In addition, levels of DDTs in the human tissues from TZ (placenta: 122 ± 109 ng/g lipid wt.; hair: 79.9 ± 215 ng/g dry wt.) were significantly higher than those from Lin'an (placenta: 49.2 ± 30.2 ng/g lipid wt.; hair: 10.8 ± 7.09 ng/g dry wt.). The above concentrations of DDTs in milk exceeded the Codex Maximum Residue Limits/Extraneous Maximum Residue Limits for milk (20 ng/g lipid wt. whole milk), indicating that the human milk samples were grossly polluted. The present study revealed that human specimens collected from the coastal city (TZ) were more contaminated with inland one (LA), based on the levels of DDTs contained in samples which may be due to the higher dietary exposure to DDTs via consumption of contaminated seafood. The estimated daily intakes of DDTs by GY, TZ and LA infants were 1.69 ± 1.86, 1.48 ± 0.79, and 0.95 ± 0.73 μg/kg body wt./day, respectively which did not exceed 10 μg/kg body wt./day, the provisional tolerable daily intake proposed by the Food and Agriculture Organization/World Health Organization.     

Cesium accumulation by fish following acute input to lakes: a comparison of experimental and Chernobyl-impacted systems

An uptake parameter u (L kg⁻¹ d⁻¹) and a loss rate parameter k (d⁻¹) were estimated for the patterns of accumulation and loss of ¹³³Cs by three fish species following an experimental ¹³³Cs addition into a pond in South Carolina, USA. These u and k parameters were compared to similar estimates for fish from other experimental ponds and from lakes that received ¹³⁷Cs deposition from Chernobyl. Estimates of u from ponds and lakes declined with increasing potassium concentrations in the water column. Although loss rates were greater in the experimental ponds, the times required to reach maximum Cs concentrations in fish were similar between ponds and lakes, because ponds and lakes had similar retentions of Cs in the water column. The maximum Cs concentrations in fish were largely determined by initial Cs concentrations in the water column. These maximum concentrations in fish and the times required to reach these maxima are potentially useful indicators for assessments of risks to humans from fish consumption.     

Levels and regional trends of persistent organochlorines and polybrominated diphenyl ethers in Asian breast milk demonstrate POPs signatures unique to individual countries

Human breast milk samples collected in 2007–2008 from four countries, Vietnam (Hanoi), China (Beijing), Korea (Seoul) and Japan (Sendai, Kyoto and Takayama), were analyzed for persistent organic pollutants (POPs) such as dichlorodiphenyltrichloroethane and its metabolites (DDTs), chlordane-related compounds (CHLs), hexachlorocyclohexanes (HCHs), hexachlorobenzene (HCB), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). Comparing with previous surveys, the present study indicates that the DDTs in breast milk from China and Vietnam had gradually decreased during the last decade, but were still 5–10 times higher than those in other nations. The ratios of p,p′-DDE/p,p′-DDT and o,p′-DDT/p,p′-DDT were higher in Beijing than in the other countries, suggesting that there is less fresh intake of commercial DDT products and a possible exposure to dicofol in China. CHL and PCB levels were relatively higher in mothers from Japan, whereas β-HCH and HCB were more common in Chinese women. In Japan, it is suspected that mothers in the urban/coastal area (Sendai) were more continuously exposed to organochlorine pesticides (OCPs) than mothers in the rural/inland area (Takayama). In addition, OCP levels in primiparae were significantly higher than those in multiparae from Japan and Korea. These indicate that both parity and regional factors are major determinants of the levels of OCPs and PCBs in human milk. On the other hand, higher concentrations of PBDEs were observed in mothers' milk from Korea. The congener was dominated by BDE-47 (43–54%), followed by BDE-153 (23–33%) in all regions except for Beijing where BDE-28 (23%) was relatively abundant. In Japanese breast milk, regional and parity-dependent distributions were not observed for PBDEs. Among PBDE congeners, age-dependency was observed for BDE-153, which was negatively correlated (p < 0.05) to the age of mothers in Kyoto (17 participants were housewives), while it increased with age in Sendai (10 participants were clerks). No such correlation was seen for BDE-47, indicating that BDE-47 was ingested and assimilated via different kinetics or routes from BDE-153 in Japan.     

Toxicokinetics of dioxins and other organochlorine compounds in Japanese people: Association with hepatic CYP1A2 expression levels

Concentrations of persistent organochlorine compounds (OCs) including polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and polychlorinated biphenyls (PCBs) in the liver and adipose tissue of Japanese cadavers were measured, and their toxicokinetics were examined in association with hepatic cytochrome P450 (CYP) 1A protein expression levels. Total 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalents (TEQs) were 66 ± 74 and 65 ± 57 pg/g lipid weight (mean ± S.D.) in the liver and adipose tissue, respectively. Total PCBs (sum of 62 congeners targeted), p,p′-dichlorodiphenyl-dichloroethylene (p,p′-DDE) and β-hexachlorocyclohexane (β-HCH) were detected at concentrations over 1 μg/g lipid in both tissues of some specimens. For most of the dioxin-like congeners, total PCBs, p,p′-DDE, oxychlordane, α- and β-HCH, hexachlorobenzene (HCB), and tris(4-chlorophenyl)methane (TCPMe), age-dependent increases in concentrations were found in the adipose tissue of males. No such age-dependent trend was observed in the liver, suggesting that there are different mechanisms underlying the hepatic concentrations of OCs. Immunoblot analyses indicated detectable expression of hepatic CYP1A2 protein, whereas no CYP1A1 protein was detected. The CYP1A2 expression levels were positively correlated with concentrations (on wet weight basis) of 2,3,4,7,8-P₅CDF, the dominant TEQ-contributed congeners in the liver, indicating the induction of this CYP. Hepatic CYP1A2 protein levels were strongly correlated with the liver to adipose concentration (L/A) ratios of PCDD/F congeners with more than 5 chlorine atoms. Together with higher concentrations of the congeners in the liver than in the adipose tissue, the observation on L/A ratios of highly chlorinated PCDD/Fs suggests that induced hepatic CYP1A2 protein is involved in their sequestration in this human population, as observed in model animals (rodents). Nonetheless, the magnitude of hepatic sequestration (L/A ratio) of PCDD/Fs in this human population was lower than in other mammals and birds, reported previously. This study emphasizes the fact that toxicokinetics of some OCs can be affected at least partly by CYP1A2 protein levels in humans. For the extrapolation of their toxicokinetics from model animals to humans, knowledge on the induction and sequestration potencies of CYP1A is necessary.     

Short- and long-term patterns of ¹³⁷Cs in fish and other aquatic organisms of small forest lakes in southern Finland since the Chernobyl accident

We summarize the patterns of ¹³⁷Cs activity concentrations and transfer into fish and other biota in four small forest lakes in southern Finland during a twenty-year period following the Chernobyl accident in April 1986. The results from summer 1986 showed fastest accumulation of ¹³⁷Cs into planktivorous fishes, i.e. along the shortest food chains. Since 1987, the highest annual mean values of ¹³⁷Cs have been recorded in fish occupying the highest trophic levels, for perch (Perca fluviatilis) 13,600 Bq/kg (ww) and for pike (Esox lucius) 20,700 Bq/kg (ww). At the same time, activity concentrations of ¹³⁷Cs in crustacean zooplankton and Asellus aquaticus have ranged between 1000 and 19,500 Bq/kg (dw). In 2006, 5-28% of the 1987 ¹³⁷Cs activity concentration levels were still present in perch and pike. Since 1989 their ¹³⁷Cs activity concentrations in oligohumic seepage lakes have remained significantly higher than in polyhumic drainage lakes due to the increased transfer of ¹³⁷Cs into fish in the seepage lakes with lower electrolyte concentrations, longer water retention times and lower sedimentation rate.     

Transfer of Sr into fish in Finnish lakes

The long-term behavior of ⁹⁰Sr was investigated from 1987 to 1997 in fifteen lakes in southern and central Finland following the 1986 Chernobyl accident. Both water and fish samples (perch, pike, vendace) were analyzed. ⁹⁰Sr stays long in the freshwater ecosystem; the observed half-lives during the study period were generally around 10 years in water and even longer in fish. One lake exhibited very different behavior for ⁹⁰Sr, with elevated levels in fish and water and very short observed half-life in fish, less than 2 years. Concentration factors of ⁹⁰Sr in fish (Bqkg⁻¹ f.w. in fish/Bqkg⁻¹ in water) among the studied lakes significantly correlated with both the Ca concentration and electrical conductivity of the water. More ⁹⁰Sr was transferred into fish in lakes with a low electrical conductivity and a low Ca concentration. Among other water parameters evaluated were pH, color, total nitrogen, and phosphorus.     

Characterization of DDT in factory effluent by thin-layer chromatography

Thin-layer chromatography (TLC) is shown as an effective, inexpensive, reliable, and less time consuming technique for simple and effective monitoring of persistent toxicant-like DDT in its manufacturing plant effluent. Aqueous untreated and treated grab and composit effluent samples were collected, extracted with a solvent and concentrated and chromatographed both for qualitative and quantitative analysis for p, p′-DDT and related compounds. This technique was successful and can form an effective, inexpensive method to be used by the developing countries, which have limited financial resources.     

Gamma radioactivity in the Iberian Marine environment closest to the NEA dumping site

The distributions of caesium-137 and some naturally occurring gamma-emitters in a range of environmental samples, collected along the north-west Iberian coastline during April 1984, are presented. Samples, including surface waters, algae, sediments and fish, have been analyzed to establish accurate baselines for that region of the continental shelf closest to the NEA dumping site. Results indicate that caesium-137 levels were a factor of three lower than those typical of waters off the west coast of Ireland and are consistent with those expected from global fallout prior to the Chernobyl accident. The mean caesium-137 concentration in sea water at six well-separated stations near the coast was 3·85 ± 0·19 Bq m⁻³, whilst caesium-137 levels in widely consumed species of fish were in the range 1·1–6·2 Bq kg⁻¹ (dry wt). It is estimated that caesium-137, through one year's ingestion of fish and shellfish, generates a collective dose equivalent commitment to the adult Galician population of 0·78 man-Sv, which is approximately 3% of the total annula collective dose equivalent from non-medical sources of radiation.     

137Cs in freshwater fish in Finland since 1986--a statistical analysis with multivariate linear regression models

The accident at the Chernobyl nuclear power plant in 1986 significantly elevated the 137Cs levels of fish in Finnish lakes. About 6200 fish samples from 390 lakes comprising 20 species have been analysed for 137Cs since 1986. The sizes of the lakes varied from a few hectares to about 1000 km2. Activity concentrations of 137Cs in fish still varied widely in 2003, from 16 to 6400 Bq/kg fresh weight. This paper presents the results of statistical analyses with multivariate linear regression models carried out on the empirical data collected since 1986. The statistical analysis resulted in separate models for two time periods describing temporal changes of 137Cs in fish. The explanatory variables were fish species with various feeding habits, the size class of the lake, municipal division, drainage area, time since the deposition and deposition level of the municipality. The calculated values for 137Cs in fish did not differ statistically significantly from the observed values in the validation data. The explanatory variables explained 58% (the first time period) and 72% (the second time period) of the total variability of 137Cs in fish.     

Effect of anthropogenic pollution on oxidative processes in the liver of fish from the Rybinsk Reservoir

The levels of total lipids, peroxidation products, and antioxidant activity in the liver were studied in fish species living in unequally polluted areas of the Rybinsk Reservoir and differing in their ecology and taxonomic position: the bream (Abramis brama L.), roach (Rutilus rutilus L.), burbot (Lota lota L.), and zander (Stizostedion lucioperca L.). The fish from polluted water areas were characterized by higher levels of total lipids and malonic dialdehyde and a deficiency of antioxidants. It is shown that the test parameters depend on the type of feeding and mode of life of the fish species.     

Comparative analysis of land use and lake water quality in rural and urban zones of south Chennai, India

Chennai city, the capital of Tamil Nadu state in South India, has been experiencing rapid expansion since the last two decades, resulting in major changes in land use and degradation of wetlands. Small lakes in the peri-urban areas face severe strain on their environment due to transition of rural to urban conditions, leaving at stake their aquatic health and intended uses. This paper studies the role of urbanization and land use changes in the water quality of peri-urban (Rajakilpakkam) and rural (Vengaivasal) lakes. Water samples were collected and analysed for temperature, total dissolved solids, major ions, nutrients and biological oxygen demand as per standard methods. The temperature, pH and biological oxygen demand did not differ between lakes, while total dissolved solids (p = 0.008), alkalinity (p = 0.000), total hardness (p = 0.001) and phosphate (p = 0.000) were significantly higher in Rajakilpakkam. Seasonal and spatial variations in water quality between the lakes showed the direct impact of rapid and uncontrolled growth of built-up areas in the catchment area, in enhancing waste water inflows with inorganic salts and nutrients in Rajakilpakkam lake compared with Vengaivasal lake. Urbanization of the catchment and encroachments in Rajakilpakkam lake tends to reduce the social interdependence of lake and community and promote disuse, leading to decline in water quality. The impending environmental costs caused by urbanization to these lakes will only be tackled, if the main issues of domestic and industrial discharges and encroachments are addressed properly.     

Circulating levels of environmental contaminants are associated with dietary patterns in older adults

BackgroundFood intake contributes substantially to our exposure to environmental contaminants. Still, little is known about our dietary habits' contribution to exposure variability.ObjectiveThe aim of this study was to assess circulating levels of environmental contaminants in relation to predefined dietary patterns in an elderly Swedish population.MethodsDietary data and serum concentrations of environmental contaminants were obtained from 844 70-year-old Swedish subjects (50% women) in the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study. Dietary data from 7-day food records was used to assess adherence to a Mediterranean-like diet, a low carbohydrate–high protein diet and the WHO dietary recommendations. Circulating levels of 6 polychlorinated biphenyl markers, 3 organochlorine pesticides, 1 dioxin and 1 polybrominated diphenyl ether, the metals cadmium, lead, mercury and aluminum and serum levels of bisphenol A and 4 phthalate metabolites were investigated in relation to dietary patterns in multivariate linear regression models.ResultsA Mediterranean-like diet was positively associated with levels of several polychlorinated biphenyls (118, 126, 153, and 209), trans-nonachlor and mercury. A low carbohydrate–high protein diet was positively associated with polychlorinated biphenyls 118 and 153, trans-nonachlor, hexachlorobenzene and p, p′-dichlorodiphenyldichloroethylene, mercury and lead. The WHO recommended diet was negatively related to levels of dioxin and lead, and borderline positively to polychlorinated biphenyl 118 and trans-nonachlor.ConclusionDietary patterns were associated in diverse manners with circulating levels of environmental contaminants in this elderly Swedish population. Following the WHO dietary recommendations seems to be associated with a lower burden of environmental contaminants.     

Anomalous behavior of trace element magnesium in fish tissues

Six fish species were collected: summer trout (Salmo alar), rock fish (Roccus lineatus), pink snapper (Lutianus ava), croaker (Stellifer lancelatus), sheepshead (Diplodus holbrooki) and spanish mackeral (Scomberomorus maculatus). Seven trace elements were determined by flame atomic absorption spectrophotometry. The results indicate that the trace elements iron, copper, zinc, mercury, chromium, and nickel show higher levels in the livers than in the flesh of all the six fish species. Surprisingly, magnesium concentration levels were consistently higher in flesh than in the livers of all six fish species.     

Characteristics of parasitic infection in the least cisco, Coregonus sardinella valenciennes, 1848, in the Ural tributaries of the lower Ob

Data on the parasite fauna of the least cisco, Coregonus sardinella Valenciennes, 1848 in Ural tributaries of the Ob downstream of the city of Salekhard have been obtained for the first time. The formation patterns of the parasite fauna in this species and the rate (prevalence and intensity) of infection by Ichthyocotylurus erraticus (Odening, 1969), a parasite specific to coregonids, have been revealed. Infection of the least cisco with six parasite species (out of the 12 species found) occurs as the fish feed on zooplankton and benthic organisms. The dominant parasites are trematode larvae of the genera Ichthyocotylurus and, in some years, Diplostomum; this composition of dominants is evidence that the least cisco fattens up mainly in shallow areas. All sexually mature fish of this species were found to be infected by I. erraticus, but the average intensity of infection was rather low: the abundance index of the parasite did not exceed 40 ind. per fish. Analysis of the distribution of I. erraticus metacercariae in the least cisco shows that the relationship in the host-parasite system is stable and the parasite has no pathogenic effect on individuals of older age classes.     

Does human activity impact the natural antibiotic resistance background? Abundance of antibiotic resistance genes in 21 Swiss lakes

Antibiotic resistance genes (ARGs) are emerging environmental contaminants, known to be continuously discharged into the aquatic environment via human and animal waste. Freshwater aquatic environments represent potential reservoirs for ARG and potentially allow sewage-derived ARG to persist and spread in the environment. This may create increased opportunities for an eventual contact with, and gene transfer to, human and animal pathogens via the food chain or drinking water. However, assessment of this risk requires a better understanding of the level and variability of the natural resistance background and the extent of the human impact. We have analyzed water samples from 21 Swiss lakes, taken at sampling points that were not under the direct influence of local contamination sources and analyzed the relative abundance of ARG using quantitative real-time PCR. Copy numbers of genes mediating resistance to three different broad-spectrum antibiotic classes (sulfonamides: sul1, sul2, tetracyclines: tet(B), tet(M), tet(W) and fluoroquinolones: qnrA) were normalized to copy numbers of bacterial 16S rRNA genes. We used multiple linear regression to assess if ARG abundance is related to human activities in the catchment, microbial community composition and the eutrophication status of the lakes. Sul genes were detected in all sampled lakes, whereas only four lakes contained quantifiable numbers of tet genes, and qnrA remained below detection in all lakes. Our data indicate higher abundance of sul1 in lakes with increasing number and capacity of wastewater treatment plants (WWTPs) in the catchment. sul2 abundance was rather related to long water residence times and eutrophication status. Our study demonstrates the potential of freshwater lakes to preserve antibiotic resistance genes, and provides a reference for ARG abundance from lake systems with low human impact as a baseline for assessing ARG contamination in lake water.     

First report of pyrethroid bioaccumulation in wild river fish: A case study in Iberian river basins (Spain)

For the first time, this work described pyrethroid bioaccumulation in edible river fish samples. We analyzed 42 whole fish samples collected in 4 different Iberian rivers. All samples were positive to these insecticides. Levels of concentration ranged from 12 to 4938 ng g^− 1 lipid weight (lw). Moreover, isomeric characterization was carried out. Our results remarked a general preference of cis isomers in bioaccumulation. Finally, the enantiomeric evaluation showed that there was an enantioselective bioaccumulation of some pyrethroids, depending on the studied species. Pyrethroid concentrations were compared with levels obtained for other common pollutants, such as flame retardants, personal care products, hormones and pharmaceuticals. The highest values corresponded to pyrethroid insecticides, even though, pyrethroid levels are safe for human consumption taken into account the current regulations.     

Influence of fish size and sex on mercury/PCB concentration: importance for fish consumption advisories

Fish advisories for polychlorinated biphenyls (total-PCBs) and mercury are often given on a length-specific basis and fish sex is usually not considered. The relationship between concentration and length is well established for mercury, however its suitability for total-PCBs and the influence of sex over the large scale covered by most monitoring programs is not well known. Here we use what is perhaps the largest consistent sport fish contaminant dataset to evaluate the relationship between total-PCB/mercury and length and sex. We evaluated seven of the most commonly consumed fishes from the Canadian Great Lakes and two Ontario (Canada) inland lakes. For mercury, the relationship between concentration and length was significant (p<0.05) in most fish species and locations. For total-PCB, this relationship was also generally significant in Chinook salmon and lake trout, which are the species with the most advisories for this compound. In contrast, significant relationships were found less often for whitefish, northern pike, smallmouth bass, walleye, and especially yellow perch. However, mercury usually drives fish consumption advisories for these species. Overall, our results support the protocol of issuing contaminant advice on a length-specific basis in fish monitoring programs with reasonable confidence when at least a moderate number of samples over the natural size range of a species are included in the analysis. Sex differences were common for walleye (males>females, p<0.05) but not other species, suggesting that an equal number of male and female walleye should be used in deriving fish advisories for walleye.     

Comparative Analysis of Variation in Ecological Forms of Altai Osman, <Emphasis Type="Italic">Oreoleuciscus potanini</Emphasis> (Cyprinidae), in Lakes Ayrag,Khyargas, and Nogon (Western Mongolia)

Analysis of variation in morphological characters of Potanin Altai osman, Oreoleuciscus potanini, from two freshwater and one brackish water lakes of Western Mongolia has shown that specimens of different ecological forms—herbivorous and piscivorous—form two integral and, at the level under consideration, internally indivisible units in the system of phenetic diversity of the species O. potanini.