Morphophysiological adaptation aspects of different Haloxylon aphyllum (Chenopodiaceae) genotypes along a salinity gradient

The seedlings of Haloxylon aphyllum from seeds with different genetic characteristics, which were collected in three natural subpopulations along a soil salinity gradient, were investigated. The plants grown under various levels of NaCl (0–300 mM) differed in the shoot length, the relative growth rate (calculated for fresh and dry biomass), as well as ions and proline contents. The heterozygous genotypes of xerohalophytic H. aphyllum have shown significant advantages in their productivity and sustainability under moderate and high salinity.     

Genetic Differentiation of the Ural Endemic <Emphasis Type="Italic">Gypsophila uralensis</Emphasis> (Caryophyllaceae) in Relict Fragments of Its Range in Northwestern European Russia

Molecular genetic methods (AFLP and ITS1–5.8S–ITS2 rDNA analysis) have been used to study the level and structure of genetic diversity in relict populations of the Ural endemic Gypsophila uralensis Less. in the northeast of European Russia. Intraspecific genetic differentiation is most clearly manifested between G. uralensis Less. subsp. pinegensis (Perf.) Kamelin locally endemic to the north of Europe (Arkhangelsk oblast, locus classicus) the population of G. uralensis Less. subsp. uralensis on limestone outcrops along the Shchugor River, the Subpolar Urals. The cluster of Timan populations (on limestone outcrops along the Svetlaya, Pizhma, and Myla rivers) is autonomous and genetically heterogeneous. Genetic distances between model G. uralensis s.l. populations are correlated with geographic distances. The size and abundance of relict populations show a descending gradient in the forest zone, and parameters of their genetic diversity [1] and unbiased expected heterozygosity have been found to decrease along this gradient.     

Avian species distribution in different elevation zone forest (Sal, Pine Mixed And Oak) in nainital district of uttarakhand, India

The present study were made to estimate the avian fauna in terms of species richness and diversity and guild structure in forest habitats of Nainital district of Uttarakhand (350–2.450 m asl; 29° N). Field studies were conducted during January 2007 to December 2008. Total 43, 62 and 42 species were recorded from, Haldwani, Bhowali and Nainital forest habitats. Results indicate the species relationship between Bird species richness (BSR) and elevation sections (forest habitats) was not decline simultaneously along elevation; it shows hump shaped. BSR varied considerably along elevational gradient (43 to 62 species), was highest (62 species) at mid elevation (Mixed pine forest, 1.350–1.700 m asl) and decreased (20 species) at high elevation (Conifer forest, 1900–2450 m asl). It seems that mid altitude bulge is not caused by the presence of a group of mid altitude specialists but rather that there is an overlap in the distribution of low land and high elevation specialists at this altitude. A checklist of 79 avian species has also produced of Nainital district forest habitats. It is suggested that this study provide a base line structure for further study on species distribution in different forest habitats and along different elevation section in Western Himalayas (India).     

Tree vegetation of the forest-tundra ecotone in the Western Sayan mountains and climatic trends

Parameters of reproduction of the Siberian stone pine (Pinus sibirica), including radial and apical tree increments, the age structure of stands, the amount of young growth, and its distribution along an altitudinal gradient, have been studied in the forest-tundra ecotone of the Western Sayan. The results show that, over the past 30 years, P. sibirica undergrowth has expanded to the mountain tundra belt, the apical and radial tree increments and stand density have increased, and the life form of many P. sibirica plants has changed from prostrate to erect (single-or multistemmed). These changes correlate with the dynamics of summer temperatures and monthly (in May and June) and annual precipitation. The rise of summer temperatures by 1°C promotes the expansion of P. sibirica undergrowth for approximately 150 m up the altitudinal gradient.     

Genetic differentiation of black saxaul, Haloxylon aphyllum (Chenopodiaceae), along a soil salinity gradient in the Kyzylkum Desert

Populations of Haloxylon àphyllum in the Kyzylkum Desert have been found to be markedly deficient in heterozygotes at a medium level of genetic diversity (P ₉₅ = 0.56, A = 1.67, H _o = 0.14, H _e = 0.28). Spatial genetic differentiation of these populations have been revealed along a soil salinity gradient (from 0 to 0.5 mmol Na⁺/g), with their genetic diversity reaching a maximum (H _o = 0.21?C0.25, H _e = 0.25?C0.27) in areas with a moderate salinity level (0.05?C0.1 mmol Na⁺/g). Locus Got-2 can serve as a marker of this differentiation (Fst_Got-2 = 0.4).     

Stable nitrogen isotopes (δ15N) in thalli of the lichen Hypogymnia physodes along a altitudinal gradient in the Khangai Plateau, Mongolia

Stable nitrogen isotopes (??¹⁵N) have been determined in the organic matter (OM) of thalli of the lichen Hypogymnia physodes (L.) Nyl. collected at 15 levels along an altitudinal gradient (1250?C2928 m a.s.l.) in forest and alpine meadow communities of the Khangai Plateau, Mongolia. The results show that, in the study region as a whole, no correlation between the ??¹⁵N content and elevation is observed. However, the lichen OM within the mountain forest belt is depleted of the heavy ¹⁵N isotope as elevation increases (from 1250 to 2400 m a.s.l.), whereas in the alpine meadow belt (2500?C2928 m a.s.l) it is enriched with this isotope. In addition, ??¹⁵N values in OM show an inverse dependence on the relative content of total nitrogen in the thalli.     

Effect of three georges reservoir (Yangzi River) on the plant species richness in drawdown zone downstream the tributary river (Pengxihe River)

In order to understand the spatial pattern of plant community and the maintenance mechanism of biodiversity in littoral zone of Three Gorges Reservoir, 110 km stretches of Pengxihe River (from estuary to the backwater of Three Gorges) were chose to study. We investigated species composition and environmental variables along 50 m long stretches of riverbank between the high-water level and the lowest summer water level, at a 5 km interval. The results showed that: (1) along longitudinal gradients, longitudinal species richness, longitudinal shrub species richness and longitudinal herb species richness were significantly related to distance to estuary; (2) along lateral gradient, there were visible different changes in all kinds of types (lateral species richness, lateral tree species richness, lateral shrub species richness, lateral herb species richness). Along lateral gradient, species richness and herb species richness first increased with the elevation then reduced with it, trees and shrubs species richness increased with the elevation. (3) Correlation analyses showed that substrate types were correlated with the longitudinal species richness, without considering the distance to estuary. Soil moisture and substrate heterogeneity were correlated with lateral, species richness. These results indicated that, along longitudinal gradient of the river, distance to estuary plays an important role in species richness, and along lateral gradient spatial heterogeneity due to inundation and flooding plays an important role in forming a new pattern of plant community in the littoral zone of Three Gorges Reservoir Region. Because of special hydrological dynamics, plant in the littoral zone of Three Gorges, are gradually form a unique spatial pattern to some extent, though it had the same change law with riparian plant of a natural river.     

Intraspecific variation in Melandrium album along a radioactive contamination gradient at the Eastern Ural radioactive trace

Specific ecogenetic features of plants and parameters of seed progeny have been studied in Melandrium album cenopopulations from test plots established along the radioactive contamination gradient in the zone of the Eastern Ural Radioactive Trace (EURT), where absorbed radiation doses in buffer and impact plots exceeded the background level by factors of 2–18 and 330–418, respectively. It has been shown that interannual variation in parameters characterizing the viability of seed progeny largely depends on fluctuations of weather conditions. This dependence is more distinct in cenopopulations from the EURT than in control samples, which is indicative of interaction between radiation and other relevant ecological factors. The radioresistance of seed progeny is contingent on its viability, being independent of radiation load at the sites of maternal plant growth. Hermaphrodite plants occur only in irradiated M. album cenopopulations. This character is inherited: all F ₁ descendants of such plants grown in a vegetation experiment had hermaphrodite flowers. The mutability of seed progeny formed in the EURT zone exceeds the background level. The occurrence and frequencies of recorded anomalies has proved to vary by years and, therefore, can be interpreted as nonheritable modifications.     

Variation in the seed progeny of smooth brome grass,Bromus inermis Leyss., under conditions of chronic irradiation in the zone of the Eastern Ural Radioactive Trace

Long-term studies have been performed on the seed progeny of B. inermis from test plots established along the radioactive contamination gradient in the zone of the Eastern Ural Radioactive Trace (EURT), where dose loads on maternal plants and embryos of seeds from chronically irradiated cenopopulations (with natural background radiation taken into account) exceed control values by factors of 2 to 107. Interannual and individual variation in the viability, mutability, and radiosensitivity of the seed progeny has been revealed. Against the background of high interpopulation variation in test parameters in the EURT zone, weather conditions appear to have no significant effect on seed quality, while such effects are appreciable in background cenopopulations. It has been shown that a positive correlation exist between the initial viability of the seed progeny and their radioresistance. Chronic irradiation in a wide dose range induces an increased frequency of different mutations and morphological modifications. The contents of low-molecular antioxidants in seedlings from impact cenopopulations proved to be higher than in the background sample. The results obtained characterize the potential of protective and restorative mechanisms acquired in the course of evolution that allow plants to survive in the changing environment under exposure to technogenic stress.     

Phylogenogeography and genotaxonomy of Pinus sylvestris L. populations

The phylogenogeographic structure, polymorphism, and differentiation of Pinus sylvestris L. have been studied for the first time by means of allozyme analysis of 143 populations along a network of transects covering the entire species range. The results show that the species in general is characterized by a significant level of differentiation, regardless of its relative uniformity in northern and central parts of the range. Nei??s genetic distances between populations and their gradients in the extreme southern parts of the range, where it has an insular pattern, are seven to eight times greater than in the northern, ??glacial?? zone. Three Pleistocene refugia for the species have been revealed in the Balkans, Southern Urals, and Northern Mongolia. Using analysis of genetic distances between 18 phylogeographic regions and an original genosystematic scale, one subspecies, five geographic races and nine geographic population groups have been distinguished in the P. sylvestris L. species structure.     

AFLP analysis to estimate the genetic contribution of parents to progeny from hybridization between Saxifraga sibirica L. and S. cernua L.

It is shown that the method of amplified fragment length polymorphism (AFLP) can be used to estimate the contribution of parent plants to the genome of the progeny from artificial crosses between Saxifraga cernua and S. sibirica. According to Nei??s (1972) genetic distances between plant groups, F₁ plants are intermediate between the parent species but closer to S. cernua, probably because its genome size is twice that of S. sibirica. Conversely, B₁ plants proved to be closer to S. sibirica, because the hybrid progeny were crossed back to this species.     

Intra- and interspecific morphological variation of some European terrestrial orchids along a latitudinal gradient

Morphological trait variation in populations of rhizomatous (Cypripedium calceolus, Listera ovata) and root-tuber orchid species (Platanthera bifolia, Gymnadenia conopsea) has been studied along a latitudinal gradient in Europe. It has been shown that the structure of shoots changes with transition from the center to the northern or southern periphery of the species range. The reduction in the number of metameres at the range periphery is more distinct in orchids with multimetameric shoots, compared to those with oligometameric shoots. The plasticity of shoot structure observed along the geographic gradient is contingent on climatic factors and can be manifested regionally in cases of long-term temperature deviations. Other morphological parameters, such as shoot height and the sizes of leaves and flowers, can serve as indicators of ecological conditions in habitats.     

The structure of genotypic diversity and the possibility of sexual reproduction in populations of <Emphasis Type="Italic">Tulipa riparia</Emphasis> (Liliaceae)

The structure of genotypic diversity in eight populations of the clonal triploid (2n = 3x = 36) tulip Tulipa riparia inhabiting floodplain communities in the Southern Urals was studied. Analysis of nine polymorphic enzyme systems (ADH, IDH, SkDH, PGI, PGM, FDH, NADHdh, C-EST, GOT) was performed to identify clones. Both monoclonal (D = 0) and polyclonal populations with multiple genotypes from 3 to 12 (D from 0.73 to 0.91) were identified. The polyclonal populations had a high level of heterozygosity. Dispersal of propagules (bulbs) along rivers and even from one bank of a river to the opposite was detected. Some clones disperse along riverbanks to the distance of hundreds meters (up to four kilometres). At the same time, there is an effect of “isolated” or “closed” populations. Almost each sequentially arranged population in one river system have its own set of genotypes. Assessment of the state of the reproductive system components indicates presence of a rare sexual reproduction supporting genetic diversity of T. riparia.     

Responses of reproduction and important value of dominant plant species in different plant functional type in Kobresia meadow to temperature increase

Responses of reproduction and IV (important value) of dominant plant species in different PFT to warming were studied at a Kobresia meadow in the Tibetan Autonomous State of Qinghai Province, China (37°29′–37°45′ N, 101°12′–101°33′ E, 3900 m asl) using the temperature gradient method formalized by the ITEX. Responses of Elymus nutans and Poa pratensis (Gramineous PFT) to increasing temperature were similar. The numbers of tillers, buds and IV increased in the chambers treated with higher temperature compared to the control without treatments (CK). Responses of Kobresia humilis and Carex alrofusca (Cyperaceae PFT) to increasing temperature were different, that is, the numbers of tillers and IV reached the maximum in different temperature among species, the numbers of buds decreased with the temperature increasing. The number of buds and IV of Lagotis brachystachya (Forbs PFT) decreased with the warming, but the number of stolons was initially large, and then decreased with increasing temperature. The number of buds of Ranunculus brotherusii (Forbs PFT) increased with the temperature increasing in the first year, but decreased in the second year; and IV decreased with the temperature warming. Under conditions of continued warming in the future, PFT structure will be significantly changed, Cyperaceae PFT dominant plant species original position will be replaced by Gramineae PFT dominant plant species. Cyperaceae plants will become the dominant species, and some species belonging to Forbs PFT will be eliminated from the community.     

Gradient Genogeographic Analysis of <Emphasis Type="Italic">Pinus sylvestris</Emphasis> L. Populations in Europe

Gradients of Nei's genetic distances between 15 samples of Pinus sylvestris L. trees were analyzed along three submeridional transects, from Scandinavia to the Mediterranean and Transcaucasia. As a result, distinct chorogenetic differentiation was revealed between the populations of this species growing in climatically specific regions of northern and southern Europe. Considerable Nei's distances (DN = 0.045–0.056) and their gradients, combined with differences in stable phenotypic characters (the composition of monoterpenes and morphological parameters of cones) determined at the Pyrenean and Caucasian mountain borders, suggest that the P. sylvestris species structure includes two South Eurasian subspecies, the Pyrenean P. sylvestris L., ssp. iberica Svoboda and the Transcaucasian P. sylvestris L., ssp. hamata (Stev.) Fomin.     

Structural Changes in the Carabid Fauna of Forest Ecosystems under a Toxic Impact

Changes in the abundance, species composition, and biomorphological structure of the carabid fauna were studied in birch and fir–spruce forests growing in a zone exposed to emissions from a copper-smelting plant in the Middle Urals. The dynamic density of carabids decreased and their species composition and the ratio of life forms changed along the pollution gradient, but the principle of organization of carabidocenoses and the structure of dominance in them remained undisturbed.     

Diffusive gradient in thin FILMS (DGT) compared with soil solution and labile uranium fraction for predicting uranium bioavailability to ryegrass

The usefulness of uranium concentration in soil solution or recovered by selective extraction as unequivocal bioavailability indices for uranium uptake by plants is still unclear. The aim of the present study was to test if the uranium concentration measured by the diffusive gradient in thin films (DGT) technique is a relevant substitute for plant uranium availability in comparison to uranium concentration in the soil solution or uranium recovered by ammonium acetate. Ryegrass (Lolium perenne L. var. Melvina) is grown in greenhouse on a range of uranium spiked soils. The DGT-recovered uranium concentration (C_DGT) was correlated with uranium concentration in the soil solution or with uranium recovered by ammonium acetate extraction. Plant uptake was better predicted by the summed soil solution concentrations of UO₂²⁺, uranyl carbonate complexes and UO₂PO₄⁻. The DGT technique did not provide significant advantages over conventional methods to predict uranium uptake by plants.     

长江上游不同地形条件下的土地利用/覆盖变化

基于1〖DK〗∶10万的1980、2000和2005年的土地利用/覆盖数据和90 m分辨率的DEM数据,采用转移概率矩阵和地形 面积频度方法,分析了1980~2005年长江上游地区不同地形因子条件下的土地利用/覆盖变化。结果表明：1）1980~2000年,草地、林地和农业用地面积变化趋势与2000~2005年相反。后5 a,可能由于退耕的土地出现反弹现象增多,草地和林地面积出现缩减而农业用地面积增加。两个分析时段主要的土地利用/覆盖类型变化趋势类似,包括农业用地向林地和草地的转化,林地向农业用地的转化以及草地向裸地的转化。2）农业用地和城镇用地主要分布于低海拔区而林地、草地则主要分布于高海拔区。低海拔区主要是退耕还林和城市化过程。高海拔区主要是林地的退化消失和草地退化沙化过程。3）农业用地在坡度段10~25°所占面积比例最大。林地用地面积比例随坡度变陡呈现规则递增,城镇用地分布趋势与之相反。近年裸地趋向分布于缓坡区。坡度0~5°的区域主要是草地退化沙化过程;5~10°坡度段主要是退耕还林过程;大于10°的区域造林工程与森林退化并存。4）各种土地利用/覆盖类型对不同坡向的适宜性相差不大。各种坡向上土地利用/覆盖类型变化类似.     

Reproduction of the Siberian spruce in the timberline ecotone of the Iremel’ Massif

Quantitative and qualitative characteristics of cones and seeds and the emergence and survival of seedlings in the Siberian spruce (Picea obovata Ledeb.) were studied in different parts of the timberline ecotone on the Iremel’ Massif, the Southern Urals, from 2003 to 2006. In the productive year 2003, a decrease in the number of cones (from 110000 to 3000 per hectare), the yield of seeds (from 13 × 10⁶ to 0.09 × 10⁶ per hectare), and their laboratory germination rate (from 34 to 14%) with an increase in elevation above sea level was recorded. In 2004, significant differences in the number of seedlings per hectare were revealed between the lower, middle, and upper parts of the ecotone (85 200, 19 800, and 0–400 ind., respectively). It has been shown that the greater part of seedlings (76–96%) perish during the first cold period. New generations of spruce trees are formed only after productive years. Seed production and seedling mortality in spruce apparently depend not only on air temperature and soil moistening but also on snow depth and soil temperature, which change more abruptly along the altitudinal gradient.     

Comparative analysis of the parasitocenoses of amphibians from the family Ranidae (Anura) in the urbanization gradient

The parasitofauna of frogs from the fam. Ranidae (R. arvalis, R. temporaria, and P. ridibundus) has been studied in the urbanization gradient. A total of 22 parasitic species belonging to the following four taxonomic groups have been found: Protozoa, Trematoda, Monogenoidea, and Nematoda. The transformation of parasitocenoses with the growth of urbanization along with the preservation of specifics typical for the adaptive subzone in each amphibian species is accompanied with their depletion as well as with an increase in the proportion of trematodes, which results in a decrease in the system stability and increases the probability of parasitic pollution of the environment.