Hepatotoxic and haematological effects of Nigerian Bonny light crude oil in male albino rats

The effect of Bonny light crude oil was assessed in adult albino rats. The rats were administered with 200, 400, and 800?mg/kg of the crude oil orally for 7 days. Fluid intake was measured daily, and the initial and final animal body weight recorded. Oral crude oil at these doses produced no effect on feed and fluid intake between the test groups and the control group. There was a significant decrease in body weight compared to the control group after 7 days of treatment with the different doses of crude oil.

The toxic effects of the liver and hematological effects were assessed. The packed cell volume (PCV) and total white blood cell (WBC) of the 200?mg/kg group were significantly decreased compared to the respective control values. Administration of 200, 400, and 800 mg/kg of Bonny light crude oil, caused a significant dose-dependent increase in glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels, but a significant decrease in the alkaline phosphatase (AP) level compared to the control. Histological examination indicates that crude oil induced severe pathologic changes in the forms of necrosis and oedema.     

Cr(VI)对脊尾白虾(Exopalaemon carinicauda)幼虾暴露和恢复期肝胰脏的SOD活性、MDA及MTs含量的影响

为了探索水体中Cr(VI)对脊尾白虾(Exopalaemon carinicauda)的毒性效应,通过Cr(VI)的暴露(15 d)和清水恢复实验(15 d),研究了Cr(VI)对脊尾白虾肝胰脏的SOD活性、MDA及MTs含量的影响。结果表明,通过15 d的暴露,5 mg·L-1(高浓度)、0.5 mg·L-1(低浓度)实验组肝胰脏的SOD均显著高于对照组水平(p0.05),15 d的清水恢复后,两个浓度实验组均能够恢复到对照组水平(p0.05)。MDA在暴露阶段呈现持续上升的趋势,且在15 d后两个浓度实验组MDA的含量均显著高于对照组水平(p0.05),15 d的清水恢复后,5 mg·L-1实验组的MDA含量依然显著高于对照组水平(p0.05),0.5mg·L-1实验组的MDA含量基本恢复到对照组水平(p0.05)。通过15 d的暴露,两个浓度实验组MTs的含量均显著高于对照组水平(p0.05),15 d的清水恢复后,两个浓度实验组MTs的含量均能够恢复到对照组水平(p0.05)。     

栀子黄色素对小鼠血脂代谢的影响

目的 探讨扼子黄色素对高脂膳食小鼠血脂水平的影响.方法 将30只小白鼠随机分为三个组：对照组,高脂模型＋扼子黄色素组、高脂模型＋柠檬黄组（0.2、0.5 mg/g体重）,喂养180 d后,眼球采血测定血清TC、TG、MDA含量.结果 扼子黄色素组血清MDA、TC、TG升高幅度较高脂模型＋柠檬黄组明显减少.结论 扼子黄色素对高脂膳食致血脂升高有阻抑作用.表2,参6.     

Evaluation of antioxidant potentials of Morinda morindoides leaf extract

This study investigated antioxidant status of animals given aqueous extract of Morinda morindoides leaves using the levels of reduced glutathione, total-thiol, vitamin C, and vitamin E as well as malondialdehyde concentrations as indices, and its in vitro antioxidant capacity. Thirty rats divided into five groups were used. Group A served as control and were administered distilled water while groups B, C, D, and E were given 100, 200, 400, and 800 mg per kilogram body weight of water-extracted constituents of M. morindoides for 28 days. Total phenolic compounds amounted to 83.6 ± 5.9 mg g^?1 gallic acid equivalent, while total flavonoid content was 9.5 ± 0.9 mg g^?1 pyrocathecol equivalent. Malondialdehyde in plasma was significantly decreased in a dose-dependent manner, ranging from 21% in groups B and C to 84% in groups D and E. Vitamins C and E were significantly increased, in group E by 91% and 17% compared with control. Total thiols and glutathione in plasma were significantly increased, with group E having 2.5-fold and 4.2-fold higher values than control.     

围生期双酚A暴露对子代大鼠脾脏免疫功能的影响

为了研究围生期双酚A(BPA)暴露对子代脾脏免疫功能的影响,选择F344大鼠,按体重随机分为对照、低、中、高4个剂量组(BPA暴露剂量分别为0、4、40、400mg·kg-1·d-1),从雌鼠妊娠第0天开始至子鼠出生后30天,每天灌胃染毒1次,观察子鼠脾脏组织形态学的变化,并采用实时定量PCR检测IL-2、IL-12、IFN-γ、TNF-α4种细胞因子mRNA表达的改变.结果表明,1)与对照组相比,低剂量组子鼠脾重、脾脏指数均显著增加(p<0.05),体重无显著变化(p>0.05);中剂量组子鼠脾脏指数显著增加(p<0.05),体重和脾重无显著变化(p>0.05);高剂量组子鼠体重、脾重均显著降低(p<0.05),脾脏指数无显著变化(p>0.05).2)与对照组相比,各暴露组子鼠脾脏IL-2、IL-12、IFN-γ、TNF-α4种细胞因子mRNA表达均显著降低(p<0.01).以上结果提示,BPA可能能够透过胎盘屏障并通过乳汁传递给子代,降低子代的机体免疫功能.     

4-硝基酚对大鼠肝脏的毒性及氧化损伤

研究环境内分泌干扰物4-硝基酚(4-nitrophenol,PNP)对大鼠肝脏功能的毒性作用及其对核因子相关因子-2(Nrf2)通路的影响.20只SD雄性大鼠随机分成4个组,分别为对照组、1、10和100 mg·kg-1体重PNP处理组,连续皮下注射28d,检测肝脏的结构变化、氧化损伤和Nrf2及其相关基因的表达情况.结果表明,与对照组相比,100 mg·kg-1PNP处理组大鼠的血清肝功能主要指标ALT、AST、AKP活性和TBIL含量显著性升高p＜005);100 mg·kg-1组肝脏GSH-PX、CAT和SOD活性显著性降低p＜005);大鼠肝脏中Nrf2及其下游基因NQOI和HO-1 mRNA表达水平在1mg·kg-1组显著升高(p＜0.05),10、100 mg·kg-1组有升高趋势;100 mg ·kg-1组肝脏显微和超微结构都发现有不同程度的损伤.结果提示,皮下注射1 mg·kg-1 PNP引起了大鼠肝脏氧化损伤,机体可能通过提高Nrf2及其相关基因mRNA的表达水平来抵抗PNP引起的肝脏损伤;皮下注射100 mg·kg-1 PNP改变了肝脏的正常生理功能,造成肝细胞超微结构病理损伤,引起肝脏毒性.     

Protective role of L-ascorbic acid against cypermethrin-induced oxidative stress and lipid peroxidation in Wistar rats

Cypermethrin is a synthetic pyrethroid insecticide used for pest control in agriculture and as an acaricide in man and animals. This study was undertaken with the objective to investigate the propensity of cypermethrin to induce oxidative stress in rats following repetitive dermal exposure and its possible attenuation by L-ascorbic acid. Results obtained showed that cypermethrin significantly (p < 0.05) increased malonaldehyde levels, activity of catalase in rat erythrocytes and plasma protein levels. Whereas, activities of glutathione (GSH), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were significantly (p < 0.05) reduced in the cypermethrin exposed rats as compared to the control. Supplementation of L-ascorbic acid in cypermethrin-exposed rats decreased lipid peroxidation of erythrocytes, total plasma protein and catalase activity significantly (p < 0.05) compared to non-cypermethrin-exposed group. However, L-ascorbic acid did not alleviate the negative effects of cypermethrin on the activities of SOD and GSH. This study revealed that the presence of L-ascorbic acid diminishes the adverse effects of cypermethrin on some oxidative stress parameters.     

Effects of different concentrations of tetracycline and oxytetracycline on the growth and ecotoxicity in lettuce北大核心CSCD

四环素类抗生素(TCs)是目前我国应用广泛、用量最大的一类抗生素,畜禽粪便和土壤中存在TCs残留,影响蔬菜作物的生长发育. TCs因水溶性较高更容易被植物转运和积累,植物对TCs耐受性机理研究仍不足.为更全面探究土壤TCs对蔬菜的毒性作用,研究不同浓度四环素(TC)和土霉素(OTC)分别对生菜的抗生素残留、生长特征及抗氧化酶系统的影响.结果显示,在0(对照)、2、10、50、250 mg/kg 5个施用水平下,生菜叶片抗生素含量逐渐增加,且土霉素含量始终大于四环素含量.与对照相比,抗生素浓度在50 mg/kg以上时对生菜生长具有显著抑制作用,其中,株高、根长、地上部和地下部鲜重与叶片TC残留量具显著负相关.生菜叶片的脯氨酸含量随浓度增加呈先增加后降低的趋势,在10 mg/kg时达到最大.并且低浓度(2 mg/kg)促进抗氧化酶基因SOD、POD21和CAT的表达,高浓度抗生素(50、250 mg/kg)对其产生抑制作用,10 mg/kg的抗生素处理对SOD、POD21和CAT基因表达的影响在抗生素种类上存在差异.本研究表明抗生素浓度超过50 mg/kg对生菜生长产生抑制作用,脯氨酸和抗氧化酶SOD、POD、CAT的转录水平及其酶活性能快速响应抗生素胁迫,可作为生菜对抗生素抗性的辅助评价指标.(图8表3参19)     

Induction of micronuclei in bone marrow cells and hepatotoxicity of one of the most common over-the-counter pyrethroid insecticide products in Nigeria

Increasing environmental pollution in the tropics is creating a breeding ground for mosquitoes, leading to increasingly frequent use of insecticides to combat home infestation of mosquitoes, the vector of malarial parasites. Household members are therefore more prone to aerosol exposure and subsequent health effects. We assessed the hepatotoxic and clastogenic effects in rats exposed to different levels of aerosols of one of the insecticides most commonly in used in Nigeria. There were significant (p?2 in the groups of rats exposed to the aerosols when compared with the control group. In addition, rats exposed to the insecticide aerosols have elevated activities of serum enzymes: γ-glutamyl transferase, aspartate amino transferase and alanine amino transferase, as well as number of micronuclei scored in the bone marrow, at levels that are significantly (p?相似文献   

Chemopreventive role of Indigofera aspalathoides against 20-methylcholanthrene-induced carcinogenesis in mouse

This investigation was undertaken to evaluate the chemopreventive effect of hydroalcoholic extract of Indigofera aspalathoides (HAIA) against 20-methylcholanthrene (20-MC)-induced carcinogenesis in mice. Tumor was induced by single subcutaneous administration of 20-MC (200 µg per mouse) in Swiss albino mice. After 24 h of 20-MC administration, HAIA was administered at the doses of 250 and 500 mg kg^?1 body weight orally for 90 consecutive days. Mice of all groups were observed for 15 weeks to record tumor incidence (fibrosarcoma) and survival time. After 15 weeks the mice were sacrificed for the estimation of hematological profiles like hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), and liver biochemical parameters, namely lipid peroxidation, reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT). HAIA treatment markedly reduced tumor incidence and prolonged the life span of sarcoma-bearing mice as compared to 20-MC control mice. Hematological profiles were significantly (p < 0.001) restored to normal levels in HAIA-treated mice as compared to 20-MC control mice. HAIA treatment significantly (p < 0.001) modulated the aforesaid liver biochemical parameters as compared to 20-MC control. The results concluded that I. aspalathoides demonstrated a remarkable chemopreventive effect in chemical-induced carcinogenesis in mouse. The potential chemopreventive action may be due to its antioxidant and detoxifying properties.     

明日叶查尔酮对小鼠H22肝癌细胞增殖活性的抑制作用

为研究明日叶查尔酮(AC)对小鼠H22+肝癌细胞增殖的抑制作用,采用如下方法进行实验:1)将50只荷H22肝癌小鼠随机分为5组,每组10只.低、中、高剂量组每日分别灌胃给予5、20和40 mg·kg-1明日叶查尔酮,肿瘤对照组给予生理盐水,环磷酰胺(CTX)组隔天腹腔注射20 mg· kg-1的CTX.饲养10 d后处...     

Accumulation, tolerance and impact of aluminium, copper and zinc on growth and nitrate reductase activity of Ceratophyllum demersum (Hornwort)

Ceratophyllum demersum (homwort) was subjected to toxic concentrations of Al (3 and 9 mg l(-1)), Zn (3 and 9 mg l(-1)) and Cu (2.5 and 7 mg l(-1)) in culture solutions for 15 days. The higher dose of Al enhanced the chlorophyll content significantly (p<0.05) in the first 6 days of treatment while other treatments caused marked reductions. Nitrate reductase activity (NRA) was significantly reduced (p<0.05) byAl, Cu and Zn toxicity and ceased completely in plants treated with Cu by the 6th day of treatment. Dry biomass and relative growth rate were reduced significantly (p<0.05) by metal treatment. Tolerance index of the plant was low for Cu (21.62 and 13.43% at low and high doses, respectively) and moderate for Zn (63.74 and 54.85%) and Al (72.83 and 68.79%). Accumulation ofAl, Zn and Cu was threefold at higher doses compared with the lower doses but the bioconcentration factors (BCF) were very low indicating that this plant is not a hyper accumulator of these metals.     

内质网应激在PFOS致大鼠肝损伤中的作用

通过全氟辛烷磺酸(PFOS)28 d大鼠经口染毒评价PFOS肝损伤效应,探讨内质网应激在PFOS毒效应中的作用。Wistar大鼠随机分组,分别以0 mg·kg~(-1)、5 mg·kg~(-1)和10 mg·kg~(-1)PFOS灌胃染毒28 d。HE染色观察大鼠肝脏形态改变。ELISA法测定各组丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)和淀粉酶(AMY)含量变化。紫外分光光度法测定肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性变化。RT-PCR检测肝脏内质网应激标志蛋白表达水平。结果表明,PFOS造成大鼠体重降低、肝重增高(P0.05),组织切片显示肝细胞出现脂质沉积。PFOS不同剂量组大鼠ALT随暴露浓度增加,分别为(50.96±10.02)U·L~(-1)、(71.73±11.55)U·L~(-1),显著高于对照组(P0.05),AST、ALP含量与对照组相比显著上升(P0.05),高剂量组AMY水平为(833.46±63.05)U·L~(-1),与对照组相比显著降低(P0.05)。GSH-Px和SOD水平随PFOS浓度增加出现了显著降低(P0.05),而MDA水平显著升高(P0.05)。内质网应激标志蛋白表达均较对照组显著上升(P0.05)。以上结果说明PFOS可导致大鼠肝细胞损伤,其机制可能与内质网应激调控有关。     

SO2衍生物对C57BL/6小鼠肝脏T细胞亚群CD4+/CD8+的影响

为探讨二氧化硫(SO2)的肝脏免疫毒理效应,利用流式细胞仪(FACS)检测技术,分析了SO2体内代谢衍生物——亚硫酸钠(Na2SO3)与亚硫酸氢钠(NaHSO3)混合液(两者摩尔比为3:1)腹腔注射染毒对C57BL/6小鼠肝脏淋巴细胞T细胞亚群CD4+和CD8+的百分数以及CD4+/CD8+细胞比值的影响.实验组剂量分别为25、100、400mg·kg-1(body weight),染毒一周后,制备肝脏淋巴细胞悬液,经特异性荧光标记的CD4(FITC)、CD8(PE)单克隆抗体染色后,采用FACS流式细胞仪检测T淋巴细胞亚群百分数.研究发现:1)染毒后所有处理组肝脏CD4+T细胞所占的百分数显著升高(p<0.05);2)CD8+T细胞所占的百分数在100mg·kg-1、400mg·kg-1染毒组显著降低(p<0.05);3)CD4+/CD8+的比值在100mg·kg-1、400mg·kg-1染毒组显著升高(p<0.01).研究结果显示:SO2体内衍生物亚硫酸钠和亚硫酸氢钠可使肝脏CD4+/CD8+T细胞的比值显著升高,即SO2衍生物可使肝脏CD4和CD8淋巴细胞比例严重失调而使机体产生免疫紊乱.     

碲化镉量子点对小鼠睾丸组织的氧化损伤（Effects of CdTe Quantum Dots on the Antioxidant Enzymes Activity and Lipid Peroxidation in Testes of Mice）

为探讨碲化镉量子点(CdTeQDs)对小鼠睾丸的急性氧化损伤作用,将20只雄性ICR小鼠随机分成4组:对照组、1d、3d、7d(3个不同时间组),采用尾静脉注射进行一次性染毒2.5μmol·kg-1CdTeQDs,对照组注射等体积生理盐水.染毒后对睾丸的脏器系数以及组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,丙二醛(MDA)含量分别进行测定,从而检测CdTeQDs对睾丸组织的急性氧化损伤作用.结果显示,与对照组相比,随染毒时间的延长,睾丸脏器系数无显著性变化(p>0.05);GSH-Px和SOD活性随染毒时间的延长呈逐渐升高趋势;而CAT活性呈逐渐降低趋势,且与对照组差异显著(p<0.01);MDA含量显著高于对照(p<0.01),且随时间变化不大.CdTeQDs染毒可导致小鼠睾丸组织氧化损伤,其损伤程度与染毒时间之间具有一定的时间-效应关系.     

Use of Flyash as Environmental and Agronomic Amendments

Coal combustion power plant flyash materials have been reported as useful soil amendments with agronomic and environmental benefits. This paper reports the efficacy of fluidized bed combustion (FBC) and flue gas desulfurization (FGD) byproducts, when amended with dairy, swine, and broiler litter manures, in reducing phosphorus (P) solubility and potential impact on water quality. At a rate of 400 g/kg, FBC reduced water soluble P by 60% for dairy and swine and 50% for broiler litter, as compared to un-treated control samples. Byproduct FGD had little effect when amended into dairy manure, but reduced water soluble P by nearly 80% when amended into swine and broiler manure at a rate of 250 g/kg. The amount of Ca added in the amendments together with pH of the mixture is the major contributing factor in soluble P reduction. Sequential extraction results showed that the flyash treatments shifted water soluble P into mainly bicarbonate extractable P. The latter is still considered available for crop uptake but less vulnerable for environmental losses. Coal combustion byproducts, when amended into manure and used properly, can provide a useful and viable option for improving nutrient management on animal farms.     

Use of flyash as environmental and agronomic amendments

Coal combustion power plant flyash materials have been reported as useful soil amendments with agronomic and environmental benefits. This paper reports the efficacy of fluidized bed combustion (FBC) and flue gas desulfurization (FGD) byproducts, when amended with dairy, swine, and broiler litter manures, in reducing phosphorus (P) solubility and potential impact on water quality. At a rate of 400 g/kg, FBC reduced water soluble P by 60% for dairy and swine and 50% for broiler litter, as compared to un-treated control samples. Byproduct FGD had little effect when amended into dairy manure, but reduced water soluble P by nearly 80% when amended into swine and broiler manure at a rate of 250 g/kg. The amount of Ca added in the amendments together with pH of the mixture is the major contributing factor in soluble P reduction. Sequential extraction results showed that the flyash treatments shifted water soluble P into mainly bicarbonate extractable P. The latter is still considered available for crop uptake but less vulnerable for environmental losses. Coal combustion byproducts, when amended into manure and used properly, can provide a useful and viable option for improving nutrient management on animal farms.     

出生前后全氟辛烷磺酸（PFOS）暴露对大鼠额叶皮质中IGF-Ⅰ、IGF-ⅠR和IGFBP-2 mRNA水平的影响

为研究出生前后不同时期全氟辛烷磺酸(PFOS)暴露对大鼠仔鼠额叶皮质胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、胰岛素样生长因子-Ⅰ受体(IGF-ⅠR)和胰岛素样生长因子结合蛋白-2(IGFBP-2)mRNA水平的影响,将28只受孕Wistar大鼠按3:2:2的比例随机分到对照(C)、低剂量(L)和高剂量(H)组中,从妊娠第0天开始分别自由摄食含0、7.2、14.4mg·kg-(1以饲料中的PFOS计)的粉末状饲料进行连续染毒至出生后28天.采用交叉哺育的方法建立出生前后均不暴露(CC)、仅出生前暴露(LC和HC)、仅出生后暴露(CL和CH)、出生前后均暴露(LL和HH)于PFOS的动物模型.采用半定量RT-PCR法检测各组仔鼠额叶皮质IGF-Ⅰ、IGF-ⅠR和IGFBP-2mRNA水平的变化.结果表明,对照组仔鼠额叶皮质IGF-Ⅰ、IGFBP-2mRNA在PD14时处于较高水平,而在PD1和PD28时水平较低;IGF-ⅠRmRNA在PD1时只处于较低水平,随年龄的增加而逐渐升高.在PD1时,暴露组仔鼠IGF-Ⅰ、IGF-ⅠR、IGFBP-2mRNA水平显著高于对照组(p<0.05).在PD14时,除LC组仔鼠IGF-ⅠRmRNA水平显著高于对照组之外,其余各暴露组仔鼠3者mRNA水平均显著低于对照组(p<0.01).此外,出生后暴露PFOS组的仔鼠额叶皮质IGF-Ⅰ、IGFBP-2mRNA水平降低程度比出生前暴露PFOS组仔鼠更高.在PD28时,暴露组仔鼠IGF-Ⅰ、IGF-ⅠRmRNA水平显著低于对照组(p<0.01),而IGFBP-2mRNA水平与对照组之间的差异无显著性(p>0.05).出生前后不同时期暴露PFOS导致大鼠仔鼠额叶皮质IGF-Ⅰ、IGF-ⅠR和IGFBP-2mRNA水平变化,为PFOS发育神经毒性的研究提供了一定的线索.     

丙烯腈诱导的氧化应激对大鼠肝脏内质网应激信号通路的影响

为探讨丙烯腈(acrylonitrile,ACN)诱导的大鼠肝脏氧化损伤对内质网应激(endoplasmic reticulum stress,ERS)信号通路的影响,我们将50只SPF级成年雄性SD大鼠按体重随机分为5组,每组10只。各组分别以12.5、25.0、50.0 mg·kg~(-1)ACN灌胃染毒,N-乙酰半胱氨酸(N-acetylcysteine,NAC)干预组先用300.0 mg·kg~(-1)NAC灌胃30 min后再灌50.0 mg·kg~(-1)ACN,对照组以0.5 mL·(100 g)~(-1)的玉米油灌胃,1次·天~(-1),6天·周~(-1),共计13周。染毒结束后,检测肝脏组织氧化还原酶活力及丙二醛(malondialdehyde,MDA)含量,GRP78、CHOP及caspase-12 mRNA及蛋白表达水平。结果显示:低、中ACN组大鼠肝脏GSH含量显著低于对照组(P0.05);低ACN组大鼠肝脏GSH-Px活力、SOD活力及MDA含量均显著高于对照组(P0.05);中、高ACN组大鼠肝脏CAT活力明显低于对照组(P0.05)。NAC干预后可逆转ACN诱导的大鼠肝脏GSH含量、MDA含量及SOD活力的变化。RT-PCR结果显示,高ACN组大鼠肝脏GRP78、CHOP、caspase-12 mRNA表达水平与对照组比较均升高(P0.05)。NAC干预后,CHOP、caspase-12 mRNA表达水平与高ACN组比较均降低(P0.05)。Western Blot结果显示,高ACN组大鼠肝脏GRP78、CHOP、caspase-12蛋白表达水平与对照组比较均升高(P0.05),NAC干预后可逆转以上作用。结果表明,ACN慢性染毒对大鼠肝脏的氧化损伤可激活ERS信号通路,NAC可减轻氧化损伤的程度而阻断ERS信号通路,这可能是ACN产生肝脏毒性的机制之一。     

3-甲基-4-硝基酚对公鸡睾丸早期发育的影响

研究环境内分泌干扰物3-甲基-4-硝基酚(3-methyl-4-nitrophenol,PNMC)对公鸡睾丸早期发育的影响。48只1 d龄白羽公鸡随机分成3个组,分别为对照组、10和100 mg·L-1PNMC处理组,连续饮水42 d。检测公鸡的生长性能、睾丸重量和指数、睾丸的结构变化、血清激素水平和睾丸中CYP17A1及其相关基因的表达情况。结果显示:与对照组相比,10 mg·L-1PNMC处理组显著降低公鸡的生长性能;睾丸重量和睾丸指数均无显著性变化;睾丸细精管生精细胞均有不同程度的空泡兼或脱落;血清孕酮和雌二醇水平均无显著性差异,睾酮水平随饮水中PNMC浓度的升高呈递减的趋势;公鸡睾丸中CYP17A1、HSD3B2和CYP19A1有升高的趋势,HSD17B4在10 mg·L-1PNMC组呈降低的趋势,在100 mg·L-1PNMC组呈升高的趋势。结果表明:饮水中添加10 mg·L-1PNMC显著影响了公鸡的生长性能;饮水中添加100 mg·L-1PNMC引起公鸡睾丸细精管生精细胞变性、坏死,造成结构病理损伤,从而对生殖系统造成损伤。