Carotenoid-dependent coloration of male American kestrels predicts ability to reduce parasitic infections

The signaling function of sexually selected traits, such as carotenoid-dependent avian plumage coloration, has received a great deal of recent attention especially with respect to parasitism and immunocompetence. We argue that parasite-mediated models of sexual selection may have an implicit temporal component that many researchers have ignored. For example, previous studies have demonstrated that carotenoid-dependent traits can signal past parasite exposure, current levels of parasitism, or the ability of individuals to manage parasitic infections in the future. We examined repeated measures of carotenoid-dependent skin color and blood parasitism in American kestrels (Falco sparverius) to distinguish whether coloration might signal current parasitism or the potential to deal with infections in the future. We found no evidence that coloration was related to current levels of parasitism in either sex. However, coloration of males significantly predicted their response to parasitism; males with bright orange coloration during prelaying, when mate choice is occurring, were more likely than dull yellow males to reduce their levels of infection by the time incubation began. Coloration during prelaying may advertise a male’s health later in the breeding season. For kestrels, the ability to predict future health would be highly beneficial given the male’s role in providing food to his mate and offspring. Coloration of females was not a significant predictor of parasitism in the future, and we provide several possible explanations for this result.     

Multimodal sexual signals in male ocellated lizards Lacerta lepida: vitamin E in scent and green coloration may signal male quality in different sensory channels

The mechanisms that confer honesty to multiple sexual signals of vertebrates remain less known. We suggest that vitamin E in the scent (femoral secretions) and carotenoids in coloration of Lacerta lepida lizards might advertise quality because they are relevant in metabolism as major antioxidants and have a dietary origin. Results showed that there may be similar trade-offs between immunity and both chemical and visual signaling, as males with more vitamin E in secretions and those with more greenish, darker, and saturated carotenoid-dependent colorations had a higher immune response. This suggests that only high-quality males can divert from metabolism and allocate enough vitamin E to secretions and enough carotenoids to coloration. Therefore, information provided by visual and chemical signals is congruent. The use of multiple sensory channels may allow signaling a male quality under different circumstances or reinforce the reliability of the signal when both signals are perceived simultaneously. However, we also found that characteristics of carotenoid-dependent coloration mirror the amounts of vitamin E in secretions. This might support that carotenoids, which are not true antioxidants in the organism, would just reflect and “inform” on the actual contents of metabolic antioxidant vitamin E, which is part of the true system that prevent oxidation. We suggest that the metabolic roles of vitamins might be the actual basis of honesty of many sexual displays, and that carotenoid-based signals might be just the visible part of the whole system.     

One trait, many signals: different information on male quality is enclosed within the same trait in a blenny fish

Colourful ornaments are traditionally evaluated as one trait. However, they could consist of several components, such as total size, colour intensity and extension, each possibly bearing its own message about one aspect of quality. Males of the blenny Salaria pavo exhibit a colourful head crest and solely care for eggs. During the breeding season, the head crest shows a yellow colouration, the intensity and relative extension of which are independent of crest size. Here, we show that: (1) carotenoids are responsible for the head crest yellow patch; (2) activating the immune system by injecting the bacterial antigen lipopolysaccharides affects both the intensity and extent of the yellow colouration; and (3) females assess males on the basis of colour patch expression. However, the response of the yellow patch to the immune challenge was dependent on head crest size. Indeed, males with a larger head crest reacted better to the simulated infection, sustaining a level of yellow patch close to pre-challenge size.     

Fear in grasslands: the effect of Eurasian kestrels on skylark abundances

Predation has received considerable theoretical and empirical support in population regulation. The effect of predators, however, could be achieved in direct (killing) or indirect effects (such as displacement). In this paper, we explored the relationship between Eurasian kestrels Falco tinnunculus and skylarks Alauda arvensis in Mediterranean grasslands. First, we analysed the presence of skylarks in the kestrel diet over 9 years. We also compared a grassland area of experimentally increased kestrel density and a second grassland as control area to evaluate the direct or indirect effect on skylark abundance. We also considered two different habitats, grazed and ungrazed plots. If skylark abundance decreased as the kestrel breeding season progressed in high-density kestrel area compared with the control area, it would suggest a direct effect (predator hypothesis). If skylark abundance remains constant in both areas of contrasting kestrel density, it would suggest that skylarks avoid kestrels (avoidance hypothesis). We found that skylark abundance decreased in the kestrel area from the beginning of kestrel nest-box installation to recent years. The rate of skylark consumption decreased in a 9-year period as kestrel abundance increased, although the total amount skylark consumption did not show a decreasing trend. In addition, skylarks were more abundant in the kestrel-free area than in the kestrel area. Finally, we found that skylark abundance did not change through the kestrel breeding period in relation to grazing. We suggest that an increased breeding density of kestrels during their breeding period may force the skylarks to breed in other areas, which may explain the decline of their abundance.     

Colorful displays signal male quality in a tropical anole lizard

Parasites influence colorful ornaments and their behavioral display in many animal hosts. Because coloration and display behavior are often critical components of communication, variation in these traits may have important implications for individual fitness, yet it remains unclear whether such traits are signals of quality in many taxa. We investigated the association between ectoparasitic mite load and the color and behavioral use of the throat fan (dewlap) by male Anolis brevirostris lizards. We found that heavily parasitized lizards exhibited lower body condition, duller dewlaps, and less frequent dewlap displays than less parasitized individuals. Our results thus suggest that highly parasitized individuals invest less in both ornamental color and behavioral display of that color. Because the two components of the signal simultaneously provide information on male quality, this study provides novel support for the long-standing hypothesis that colorful traits may function as social or sexual signals in reptiles.     

Parasites and health affect multiple sexual signals in male common wall lizards, Podarcis muralis

Multiple advertising sexual traits may either advertise different characteristics of male condition or be redundant to reinforce reliability of signals. Research has focused on multiple visual traits. However, in animals that use different multiple additional sensory systems, such as chemoreception, different types of traits might have evolved to signal similar characteristics of a male quality using different sensory channels. We examined whether ventral coloration and chemicals in femoral gland secretions of male common wall lizards, Podarcis muralis, are affected by their health state (blood-parasite load and cell-mediated immune response). Our results indicated that less parasitized lizards had brighter and more yellowish ventral colorations and also femoral secretions with higher proportions of two esters of octadecenoic acid. In addition, lizards with a greater immune response had more saturated coloration and secretions with higher proportions of octadecenoic acid methyl ester. We suggest that these signals would be reliable because only healthier males seemed able to allocate more carotenoids to coloration and presumably costly chemicals to secretions. The use of multiple sensory channels may provide more opportunities to signal a male quality under different circumstances, but also may reinforce the reliability of the signal when both types of traits may be perceived simultaneously.     

Carotenoids,immune response and the expression of sexual ornaments in male greenfinches (<Emphasis Type="Italic">Carduelis chloris</Emphasis>)

Allocation trade-offs of carotenoids between their use in the immune system and production of sexual ornaments have been suggested as a proximate mechanism maintaining honesty of sexual signals. To test this idea, we experimentally examined whether carotenoid availability in the diet was related to variation in antibody response to novel antigens in male greenfinches (Carduelis chloris aurantiiventris), a species with extensive carotenoid-dependent plumage colouration. We also measured the cost of mounting a humoral response in terms of circulating carotenoids. Finally, we examined the relationship between plumage colour, immune response and circulating carotenoids. We found that males with carotenoid-supplemented diets showed stronger antibody response than non-supplemented birds. We also found that activation of the immune system significantly reduced circulating carotenoids (24.9% lower in immune-challenged birds than in control birds). Finally, intensity (chroma) of ventral plumage colouration of males, a character directly related to concentration of total carotenoids in feathers, was negatively correlated with the immune response and circulating carotenoids in winter. These results support the idea that carotenoids are a limiting resource and that males trade ornamental colouration against immune response.     

Physiological response to etho-ecological stressors in male Alpine chamois: timescale matters!

From a life history perspective, glucocorticoids secreted by the neuroendocrine system, integrating different sources of stress through an adaptive feedback mechanism, may have important consequences on individual fitness. Although stress responses have been the object of several investigations, few studies have explored the role of proximate mechanisms responsible for the potential trade-offs between physiological stress and life history traits integrating social and environmental stressors. In 2011 and 2012, we collected data on faecal cortisol metabolites (FCM) in a marked male population of Alpine chamois, within the Gran Paradiso National Park (Italy). Using a model selection approach we analysed the effect of potential etho-ecological stressors such as age, social status (territorial vs. non-territorial males), minimum temperature, snow depth and precipitation on FCM variation. To correctly interpret environmentally and socially induced stress responses, we conducted model selections over multiple temporal scales defined a priori: year, cold months, spring, warm months, mating season. Over the year, FCM levels showed a negative relationship with minimum temperature, but altogether, climatic stressors had negligible effects on glucocorticoid secretion, possibly owing to good adaptations of chamois to severe weather conditions. Age was negatively related to FCM during the rut, possibly due to greater experience of older males in agonistic contests. Social status was an important determinant of FCM excretion: while both the ‘stress of subordination’ and the ‘stress of domination’ hypotheses received some support in spring and during the mating season, respectively, previous data suggest that only the latter may have detrimental fitness consequences on male chamois.     

青藏高原北缘银石山地区差异隆升机制

青藏高原北缘银石山地区晚新生代区域构造体制总体为南北向挤压，其主要构造表现有EW向断裂发育及其逆冲活动导致断块的差异抬升、渐新世阿克塔什组中两期挤压褶皱构造、新构造平移断裂发育及其形成多种类型的走滑成因型湖泊等。该地区详细的野外地质调查未发现晚新生代伸展构造形迹。其新构造特征的形成机制可解释为：晚新生代岩石圈地幔的拆沉及软流圈地幔的上隆使上部岩石圈破裂和抬升，同时印度板块与塔里木板块持续的南北向挤压作用使测区总体处于强烈的区域挤压应力场中，从而使表壳地块沿破裂面产生逆冲运动，并造成差异隆升。     

How to get fat: nutritional mechanisms of seasonal fat accumulation in migratory songbirds

Many migratory birds accumulate large amounts of lipids as the prime energy source for their long-distance flights. This fat accumulation is mostly under endogenous control, reflecting genetically programmed temporal shifts of the body mass set point. It is accompanied by an increase in daily food intake and food utilisation efficiency and by a seasonal shift in food selection. In particular, seasonal frugivory appears to play a key role in many migrants. Fruits have a high content of fatty acids indispensable for building up the specific depot lipids. In addition, plant secondary compounds seem to play some kind of supportive role, but the mechanisms are not yet known. The effect of being fat on the metabolic situation in migrant birds appears to be similar to the metabolic syndrome in obese humans. The fat migratory bird provides a model through which to study nutritional factors as well as the biochemical and endocrine regulation of food intake, body mass and obesity.     

Botanical composition, soil and forage quality under different management regimes in Russian grasslands

Little is known on how management of Russian native grasslands affects botanical composition, soil and forage properties. Three fields were sampled in the V.V. Alekhin Central-Chernozem Biosphere State Reserve in the Kursk region of Russia: a native grassland (not cultivated for at least 300 years), a grazed/hay field with 4 years of annual harvest followed by 1 year of rest (periodically-cut grazed/hay field), and a yearly-cut grazed/hay field. Soil samples were collected from the top 10 cm and analyzed. Plant species were identified at the sampling sites and this plant material was used to determine total elemental analysis of forage, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), in vitro true digestibility (IVTD) and lignin concentrations. Above-ground live and dead plant material and roots were analyzed for C, N and lignin. Soil sample analysis showed that fields were comparable in terms of soil chemical and physical properties. SOC and N contents were not statistically different in the native and yearly grazed/hay fields. Soil bulk density significantly increased as a result of utilization, from 0.80±0.09 Mg m⁻³ for the native grassland to 0.97±0.06 Mg m⁻³ for the yearly grazed/hay field. A total of 107 different plant species were recorded at the three fields. There were changes in plant composition among the fields. The native grassland field had the least number of plant species (41) followed by the yearly-cut grazed/hay field (68), and the periodically-cut grazed/hay field (87). There was a greater proportion of grass species (20%) in the native grassland field. Dead plant biomass and roots from the grazed/hay fields were higher in N and lignin concentrations. Forage mineral concentration was highest in the periodically-cut hay field. No significant differences were observed in terms of forage properties.     

Songs of two starling species: common traits versus adaptations to the social environment

We analysed, for the first time, songs of the African Red-winged Starling Onychognathus morio and compared their general characteristics with those of the European Starling Sturnus vulgaris. Both species are gregarious during the non-breeding season, but European Starlings tend to nest in colonies, form unstable pair-bonds and are occasionally polygynous, whereas Red-winged Starlings form long-term pair-bonds and occupy exclusive nesting territories. Red-winged Starlings produced the same basic song categories as European Starlings: warbles and whistles. These two categories appeared to be involved in similar social interactions in the two species. However, several aspects of song behaviour differed between the two species: Red-winged Starlings, breeding in isolated nests, preferentially used whistles for long-distance communication and showed a simpler organization of warbling song. Whistles in the Red-winged Starling were mostly shared between birds and, in contrast to the European Starling, were not indicators of individual identity. Also in contrast to the European Starling, female song in Red-winged Starlings appeared very important throughout the breeding period. Our results suggest that some song characteristics in the two species are phylogenetically conserved whereas others are affected by the distinct social systems of the two species.     

Gestational exposure to hydroxyprogesterone caproate suppresses reproductive potential in male rats

Hydroxyprogesterone caproate was administered to pregnant rats at a dose level of 10 and 25 mg/kg body weight on 1st, 7th and 14th gestational day and the male pups (F1 generation) were allowed to grow for 90 days. The effect of gestational exposure to hydroxyprogesterone caproate on fertility was assessed by breeding F1 male rats with control female rats besides analyzing sperm quality and quantity in F1 male rats. The number of implantation sites and viable fetuses was significantly reduced in females mated with F1 males that were exposed to hydroxyprogesterone caproate during embryonic development. The decrease in sperm function was associated with a decrease in sperm motility, sperm viability and sperm count in F1 rats. The study clearly indicates that in utero exposure to hydroxyprogesterone caproate affects fertility in male rats.     

中国广东核电集团大亚湾核电基地向游人开放

中国广东核电集团2006年12月1日宣布,将向游人开放包括大亚湾核电站和岭澳核电站在内的大亚湾核电基地,以增进公众对核电的认识和理解,普及核电知识.     

Impairment of male reproduction in adult rats exposed to hydroxyprogesterone caproate in utero

Hydroxyprogesterone caproate is one of the most effective and widely used drugs for the treatment of uterine bleeding and threatened miscarriage in women. Hydroxyprogesterone caproate was administered to pregnant rats in order to assess the effect of intraperitoneal exposure to supranormal levels of hydroxyprogesterone caproate on the male reproductive potential in the first generation. The cauda epididymal sperm count and motility decreased significantly in rats exposed to hydroxyprogesterone caproate during embryonic development, when compared with control rats. The levels of serum testosterone decreased with an increase in follicle stimulating hormone and luteinizing hormone in adult rats exposed to hydroxyprogesterone caproate during the embryonic stage. It was suggested that the impairment of male reproductive performance could be mediated through the inhibition of testosterone production.     

Applying integrated assessment methodologies to air quality plans: Two European cases

Air pollution Integrated Assessment Models (IAM) can be used for determining how emissions should be reduced to improve air quality and to protect human health in a cost-efficient way. The application of IAM is also useful to spread information to the general public and to explain the effectiveness of proposed Air Quality Plans. In this paper, the application of the RIAT+ system to determine suitable abatement measures to improve the air quality at a regional/local level is presented for two European cases: the Brussels Capital Region (Belgium) and the Porto Urban Area (Portugal). Both regions are affected with PM10 or NO₂ concentrations that exceed the limit values specified by the European Union legislation. To properly assess air quality abatement measures a surrogate model was used, allowing the implementation of an efficient optimization procedure. This model is derived in both cases through a set of simulations performed using a Chemistry Transport Model fed with different emission reduction scenarios. In addition, internal costs (due to the implementation of emission reduction measures) and external costs (due to population exposure to air pollutant concentrations) of policy options were considered. The application of this integrated assessment modelling system in scenario (Brussels case) and optimization (Porto) modes contributes to identifying some advantages and limitations of these two approaches and also provides some guidance when urban air quality has to be assessed.     

Effect of prenatal exposure to hydroxyprogesterone on steroidogenic enzymes in male rats

Pregnant Wistar strain albino rats were administered hydroxyprogesterone and the steroidogenic potential of the testis was analysed in the next generation adult male rats. In utero exposure to supranormal levels (10 mg/kg body weight or 25 mg/kg body weight) of hydroxyprogesterone decreased the activity levels of marker steroidogenic enzymes (3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase) of adult (mature) rat testis. Hydroxyprogesterone mediation in the suppression of rat testicular steroidogenesis is suggested, which can be correlated to the observed decrease in male reproductive potential in mammals exposed to female hormones during embryonic development.     

Sulfate-containing amendments to reduce methane emissions from rice fields: mechanisms,effectiveness and costs

Application of sulfate-containing amendments is oftensuggested as a mitigation option to reduce methane (CH₄) emissionsfrom rice (Oryza) fields. This paper discusses the mechanism andpotential of this mitigation option, reviews the relevant experimental data,and presents first, indicative costs of application. CH₄ emission datafor rice fields with sulfate-containing amendments are compiled toreinterpret the resulting reduction in CH₄ emission and find a generalrelationship between emission reduction and amount of sulfate applied. Thereduction in CH₄ emission depends on the amount of sulfate applied.However, absolute emission reduction is location specific and cannot bederived from the amount of sulfate (SO^2- ₄) applied only. We established alogarithmic relationship, across locations, between SO^2- ₄ application andfractional emission reduction relative to the emission of the non-amendedcontrol field. Recycling of SO^2- ₄ in the rhizosphere was essential to explainthe observed reductions in CH₄ emission for a number of theexperiments. The cost of applying SO^2- _{_4}-containing fertilizers varies acrosscountries and depends on local fertilizer prices. Since a fractional reductionis obtained, the cost-efficiency in terms of CH₄ mitigation per unitof SO^2- ₄ applied will be highest in high-emitting rice production systems.Provided the proper target areas are selected, the cost of SO^2- ₄-containingfertilizer as a mitigation option to reduce CH₄ emissions in rice fieldsis estimated at 5–10 US dollar per Mg CO₂-equivalent.