Estrogenic Compounds Downstream From Three Small Cities in Eastern Nebraska: Occurrence and Biological Effect1

Abstract: Recent studies have detected estrogenic compounds in surface waters in North America and Europe. Furthermore, the presence of estrogenic compounds in surface waters has been attributed, in some cases, to the discharge of wastewater treatment plant (WWTP) effluent. The primary objective of the current study was to determine if WWTP effluent contributes estrogens to the surface waters of Nebraska. A second objective of this study was to determine if estrogens were found in concentrations sufficient enough to manifest feminizing effects on fish. These objectives were satisfied by deploying polar organic chemical integrative samplers (POCIS) and caged fathead minnows at eight field sites. Deployment sites included: three reference sites (Pawnee Creek, the Little Blue River, and the Middle Loup River), two sites upstream of the WWTPs at Grand Island and Columbus, and three sites downstream of the WWTPs at Grand Island, Columbus, and Hastings. Following the seven day deployments, POCIS extracts were analyzed for estrone, 17β‐estradiol, estriol and 17α‐ethinylestradiol using liquid chromatography tandem mass spectrometry (LC/MS/MS). 17β‐estradiol was detected in POCIS from six of the eight field sites with the greatest quantities recovered in POCIS deployed downstream from the Grand Island and Hastings WWTPs. Estrone was detected only in the POCIS deployed downstream from the Grand Island and Hastings WWTPs. Estrogenic effects were detected in caged minnows analyzed for the hepatic mRNA expression of two estrogen‐responsive genes, vitellogenin (vg1) and estrogen receptor α (ERα). Fish deployed at the site where the greatest quantities of estrogens were recovered (Hastings) had significantly higher expression of both vg1 and ERα than fish deployed at any of the other sites. These results confirm that WWTP effluent contributes biologically significant levels of estrogens to Nebraska surface waters.     

Pharmaceuticals and other organic chemicals in selected north-central and northwestern Arkansas streams

Recently, our attention has focused on the low level detection of many antibiotics, pharmaceuticals, and other organic chemicals in water resources. The limited studies available suggest that urban or rural streams receiving wastewater effluent are more susceptible to contamination. The purpose of this study was to evaluate the occurrence of antibiotics, pharmaceuticals, and other organic chemicals at 18 sites on seven selected streams in Arkansas, USA, during March, April, and August 2004. Water samples were collected upstream and downstream from the influence of effluent discharges in northwestern Arkansas and at one site on a relatively undeveloped stream in north-central Arkansas. At least one antibiotic, pharmaceutical, or other organic chemical was detected at all sites, except at Spavinaw Creek near Mayesville, Arkansas. The greatest number of detections was observed at Mud Creek downstream from an effluent discharge, including 31 pharmaceuticals and other organic chemicals. The detection of these chemicals occurred in higher frequency at sites downstream from effluent discharges compared to those sites upstream from effluent discharges; total chemical concentration was also greater downstream. Wastewater effluent discharge increased the concentrations of detergent metabolites, fire retardants, fragrances and flavors, and steroids in these streams. Antibiotics and associated degradation products were only found at two streams downstream from effluent discharges. Overall, 42 of the 108 chemicals targeted in this study were found in water samples from at least one site, and the most frequently detected organic chemicals included caffeine, phenol, para-cresol, and acetyl hexamethyl tetrahydro naphthalene (AHTN).     

Lagrangian Sampling for Emerging Contaminants Through an Urban Stream Corridor in Colorado1

Abstract: Recent national concerns regarding the environmental occurrence of emerging contaminants (ECs) have catalyzed a series of recent studies. Many ECs are released into the environment through discharges from wastewater treatment plants (WWTPs) and other sources. In 2005, the U.S. Geological Survey and the City of Longmont initiated an investigation of selected ECs in a 13.8‐km reach of St. Vrain Creek, Colorado. Seven sites were sampled for ECs following a Lagrangian design; sites were located upstream, downstream, and in the outfall of the Longmont WWTP, and at the mouths of two tributaries, Left Hand Creek and Boulder Creek (which is influenced by multiple WWTP outfalls). Samples for 61 ECs in 16 chemical use categories were analyzed and 36 were detected in one or more samples. Of these, 16 have known or suspected endocrine‐disrupting potential. At and downstream from the WWTP outfall, detergent metabolites, fire retardants, and steroids were detected at the highest concentrations, which commonly exceeded 1 μg/l in 2005 and 2 μg/l in 2006. Most individual ECs were measured at concentrations less than 2 μg/l. The results indicate that outfalls from WWTPs are the largest but may not be the sole source of ECs in St. Vrain Creek. In 2005, high discharge was associated with fewer EC detections, lower total EC concentrations, and smaller EC loads in St. Vrain Creek and its tributaries as compared with 2006. EC behavior differed by individual compound, and some differences between sites could be attributed to analytical variability or to other factors such as physical or chemical characteristics or distance from contributing sources. Loads of some ECs, such as diethoxynonylphenol, accumulated or attenuated depending on location, discharge, and distance downstream from the WWTP, whereas others, such as bisphenol A, were largely conservative. The extent to which ECs in St. Vrain Creek affect native fish species and macroinvertebrate communities is unknown, but recent studies have shown that fish respond to very low concentrations of ECs, and further study on the fate and transport of these contaminants in the aquatic environment is warranted.     

Wastewater Effluent,Combined Sewer Overflows,and Other Sources of Organic Compounds to Lake Champlain1

Abstract: Some sources of organic wastewater compounds (OWCs) to streams, lakes, and estuaries, including wastewater‐treatment‐plant effluent, have been well documented, but other sources, particularly wet‐weather discharges from combined‐sewer‐overflow (CSO) and urban runoff, may also be major sources of OWCs. Samples of wastewater‐treatment‐plant (WWTP) effluent, CSO effluent, urban streams, large rivers, a reference (undeveloped) stream, and Lake Champlain were collected from March to August 2006. The highest concentrations of many OWCs associated with wastewater were in WWTP‐effluent samples, but high concentrations of some OWCs in samples of CSO effluent and storm runoff from urban streams subject to leaky sewer pipes or CSOs were also detected. Total concentrations and numbers of compounds detected differed substantially among sampling sites. The highest total OWC concentrations (10‐100 μg/l) were in samples of WWTP and CSO effluent. Total OWC concentrations in samples from urban streams ranged from 0.1 to 10 μg/l, and urban stream‐stormflow samples had higher concentrations than baseflow samples because of contributions of OWCs from CSOs and leaking sewer pipes. The relations between OWC concentrations in WWTP‐effluent and those in CSO effluent and urban streams varied with the degree to which the compound is removed through normal wastewater treatment. Concentrations of compounds that are highly removed during normal wastewater treatment [including caffeine, Tris(2‐butoxyethyl)phosphate, and cholesterol] were generally similar to or higher in CSO effluent than in WWTP effluent (and ranged from around 1 to over 10 μg/l) because CSO effluent is untreated, and were higher in urban‐stream stormflow samples than in baseflow samples as a result of CSO discharge and leakage from near‐surface sources during storms. Concentrations of compounds that are poorly removed during treatment, by contrast, are higher in WWTP effluent than in CSO, due to dilution. Results indicate that CSO effluent and urban stormwaters can be a significant major source of OWCs entering large water bodies such as Burlington Bay.     

Assessment of Endocrine‐Disrupting Chemicals Attenuation in a Coastal Plain Stream Prior to Wastewater Treatment Plant Closure

The U.S. Geological Survey is conducting a combined pre/post‐closure assessment at a long‐term wastewater treatment plant (WWTP) site at Fort Gordon near Augusta, Georgia. Here, we assess select endocrine‐active chemicals and benthic macroinvertebrate community structure prior to closure of the WWTP. Substantial downstream transport and limited instream attenuation of endocrine‐disrupting chemicals (EDCs) was observed in Spirit Creek over a 2.2‐km stream segment downstream of the WWTP outfall. A modest decline (less than 20% in all cases) in surface water detections was observed with increasing distance downstream of the WWTP and attributed to partitioning to the sediment. Estrogens detected in surface water in this study included estrone (E1), 17β‐estradiol (E2), and estriol (E3). The 5 ng/l and higher mean estrogen concentrations observed in downstream locations indicated that the potential for endocrine disruption was substantial. Concentrations of alkylphenol ethoxylate (APE) metabolite EDCs also remained statistically elevated above levels observed at the upstream control site. Wastewater‐derived pharmaceutical and APE metabolites were detected in the outflow of Spirit Lake, indicating the potential for EDC transport to aquatic ecosystems downstream of Fort Gordon. The results indicate substantial EDC occurrence, downstream transport, and persistence under continuous supply conditions and provide a baseline for a rare evaluation of ecosystem response to WWTP closure.     

Transport and metabolic fate of sewage particles in a recipient stream

Although the implementation of wastewater treatment plants (WWTP) has dramatically increased the quality of surface waters in urbanized areas, WWTPs can still discharge noticeable amounts of solutes and particles to recipient streams. Although the fate of WWTP nutrients has received considerable attention, transport and in-stream transformation of sewage-derived particulate organic matter (SDPOM) have not. To investigate the transport and transformation of SDPOM in recipient streams, we experimentally injected fluorescently labeled SDPOM into a headwater stream and tracked its downstream fate at baseflow. Most SDPOM disappeared from the streamwater within a 160-m long reach with an average deposition velocity of 0.14 mm s(-1). We further coupled hydrometric measurements of specific water fluxes through the streambed interface with a mixing model to estimate streambed oxygen removal, and found significantly higher oxygen removal in the deposition (0.75 g O2 m(-2) d(-1)) than in the downstream post-deposition (0.36 g O2 m(-2) d(-1)) subreach. Contrary to our expectations, we did not detect any apparent effect of SDPOM deposition on streambed clogging. Our results show the capacity of a recipient stream to retain SDPOM and to reduce its downstream export, and thus contribute to a better understanding of ecosystem services of human-altered streams.     

Nutrient Uptake in a Large Urban River1

Abstract: Small streams have been shown to be efficient in retaining nutrients and regulating downstream nutrient fluxes, but less is known about nutrient retention in larger rivers. We quantified nutrient uptake length and uptake velocity in a regulated urban river to determine the river’s ability to retain nutrients associated with wastewater treatment plant (WWTP) effluent. We measured net uptake of soluble reactive phosphorus (SRP), dissolved organic phosphorus, ammonium (NH₄), nitrate, and dissolved organic nitrogen in the Chattahoochee River, Atlanta, GA by following the downstream decline of nutrients and fluoride from WWTP effluent on 10 dates under low flow conditions. Uptake of all nutrients was sporadic. On many dates, there was no evidence of measurable nutrient uptake lengths within the reach; indeed, on several dates release of inorganic N and P within the sample reach led to increased nutrient export downstream. When uptake occurred, SRP uptake length was negatively correlated with total suspended solids and temperature. Uptake velocities of SRP and NH₄ in the Chattahoochee River were lower than velocities in less‐modified systems, but they were similar to those measured in other WWTP impacted systems. Lower uptake velocities indicate a diminished capacity for nutrient uptake.     

Chemical transport from paired agricultural and restored prairie watersheds

A five-year record of streamflow and chemical sampling data was evaluated to assess the effects of large-scale prairie restoration on transport of NO3-N, Cl, and SO4 loads from paired 5,000-ha watersheds located in Jasper County, Iowa. Water quality conditions monitored during land use conversion from row crop agriculture to native prairie in the Walnut Creek watershed were compared with a highly agricultural control watershed (Squaw Creek). Combining hydrograph separation with a load estimation program, baseflow and stormflow loads of NO3-N, Cl, and SO4 were estimated at upstream and downstream sites on Walnut Creek and a downstream site on Squaw Creek. Chemical export in both watersheds was found to occur primarily with baseflow, with baseflow transport greatest during the late summer and fall. Lower Walnut Creek watershed, which contained the restored prairie areas, exported less NO3-N and Cl compared with upper Walnut Creek and Squaw Creek watersheds. Average flow-weighted concentrations of NO3-N exceeded 10 mg/L in upper Walnut Creek and Squaw Creek, but were estimated to be 6.6 mg/L in lower Walnut Creek. Study results demonstrate the utility of partitioning loads into baseflow and stormflow components to identify sources of pollutant loading to streams.     

Nutrient retention efficiency in streams receiving inputs from wastewater treatment plants

We tested the effect of nutrient inputs from wastewater treatment plants (WWTPs) on stream nutrient retention efficiency by examining the longitudinal patterns of ammonium, nitrate, and phosphate concentrations downstream of WWTP effluents in 15 streams throughout Catalonia (Spain). We hypothesized that large nutrient loadings would saturate stream communities, lowering nutrient retention efficiency (i.e., nutrient retention relative to nutrient flux) relative to less polluted streams. Longitudinal variation in ambient nutrient concentration reflected the net result of physical, chemical, or biological uptake and release processes. Therefore, gradual increases in nutrient concentration indicate that the stream acts as a net source of nutrients to downstream environments, whereas gradual declines indicate that the stream acts as a net sink. In those streams where gradual declines in nutrient concentration were observed, we calculated the nutrient uptake length as an indicator of the stream nutrient retention efficiency. No significant decline was found in dilution-corrected concentrations of dissolved inorganic nitrogen (DIN) and phosphate in 40 and 45% of streams, respectively. In the remaining streams, uptake length (estimated based on the decline of nutrient concentrations at ambient levels) ranged from 0.14 to 29 km (DIN), and from 0.14 to 14 km (phosphate). Overall, these values are longer (lower retention efficiency) than those from nonpolluted streams of similar size, supporting our hypothesis, and suggest that high nutrient loads affect fluvial ecosystem function. This study demonstrates that the efficiency of stream ecosystems to remove nutrients has limitations because it can be significantly altered by the quantity and quality of the receiving water.     

LIMNOLOGY OF DUFFIN CREEK AND THE LOCATION OF CENTURY CITY1

ABSTRACT: Limnological study of Duffin Creek was carried out with the objective of evaluating the impact of the proposed location of Century City. The chemical, biological and bacteriological parameters of one branch of the Creek, which received tertiary treated effluent, were found to be fairly high. Considering the flow conditions of the Creek in relation to the total discharge of the treated effluent from the proposed Century City, it was recommended that the effluent be piped 3–4 miles downstream. The plan for Century City has since been abandoned     

Respective contributions of point and non-point sources of E. coli and enterococci in a large urbanized watershed (the Seine river, France)

Because the large rivers of the Seine watershed have a low microbiological water quality, the main sources of fecal contamination were investigated in the present study. The inputs of the point (wastewater treatment plants (WWTPs) effluents) and non-point sources (surface runoff and soil leaching) of fecal bacteria were quantified for Escherichia coli and intestinal enteroccoci used as bacterial indicators. In order to assess the contamination through non-point sources, fecal indicators abundance was estimated in samples collected in small streams located in rural areas upstream from all point sources; these small rivers were characterized by the land use of their watershed. Bacterial indicator numbers were also measured in effluents of WWTPs, some using classical treatment (settling followed by activated sludge process) and some using an additional disinfection stage (UV irradiation). These data were used to estimate the respective importance of each type of source at the scale of the whole Seine river watershed taking into account the land use and the population density. It shows the predominant importance of the point sources of fecal indicator bacteria at the scale of the whole watershed. In a scenario in which activated sludge treatment would be complemented with UV in all WWTPs located in this watershed, the non-point sources of fecal indicator bacteria would be dominant.     

EFFECTS OF WATERSHED BEST MANAGEMENT PRACTICES ON HABITAT AND FISH IN WISCONSIN STREAMS1

ABSTRACT: We evaluated the effectiveness of watershed‐scale implementations of best‐management practices (BMPs) for improving habitat and fish attributes in two coldwater stream systems in Wisconsin. We sampled physical habitat, water temperature, and fish communities in multiple paired treatment and reference streams before and after upland (barnyard runoff controls, manure storage, contour plowing, reduced tillage) and riparian (stream bank fencing, sloping, limited rip‐rapping) BMP installation in the treatment subwatersheds. In Spring Creek, BMPs significantly improved overall stream habitat quality, bank stability, instream cover for fish, abundance of cool‐ and coldwater fishes, and abundance of all fishes. Improvements were most pronounced at sites with riparian BMPs. Water temperatures were consistently cold enough to support coldwater fishes such as trout (Salmonidae) and sculpins (Cottidae) even before BMP installation. We observed the first‐time occurrence of naturally reproduced brown trout (Salmo trutta) in Spring Creek, indicating that the stream condition had been improved to be able to partially sustain a trout population. In Eagle Creek and its tributary Joos Creek, limited riparian BMPs led to localized gains in overall habitat quality, bank stability, and water depth. However, because few upland BMPs were installed in the subwatershed there were no improvements in water temperature or the quality of the fish community. Temperatures remained marginal for coldwater fish throughout the study. Our results demonstrate that riparian BMPs can improve habitat conditions in Wisconsin streams, but cannot restore coldwater fish communities if there is insufficient upland BMP implementation. Our approach of studying multiple paired treatment and reference streams before and after BMP implementation proved effective in detecting the response of stream ecosystems to watershed management activities.     

Spatial and Temporal Patterns of Endocrine Active Chemicals in Small Streams Indicate Differential Exposure to Aquatic Organisms

Alkylphenolic chemicals (APCs) and hormones were measured six times from February through October 2007 in three Minnesota streams receiving wastewater to identify spatial and temporal patterns in concentrations and in estrogen equivalency. Fish were collected once during the study to evaluate endpoints indicative of endocrine disruption. The most commonly detected APCs were 4‐tert‐octylphenol and 4‐nonylphenol and the most commonly detected hormones were estrone and androstenedione. Chemical concentrations were greatest for nonylphenol ethoxycarboxylates (NPECs) (5,000‐140,000 ng/l), followed by 4‐nonlylphenol and 4‐nonylphenolethoxylates (50‐880 ng/l), 4‐tert‐octylphenol and 4‐tert‐octylphenolethoxylates with concentrations as great as 130 ng/l, and hormones (0.1‐54 ng/l). Patterns in chemicals and estrogen equivalency indicated that wastewater effluent is a pathway of APCs and hormones to downstream locations in this study. However, upstream contributions can be equally or more important indicating alternative sources. This study indicates that aquatic organisms experience both spatially and temporally variable exposures in the number of compounds, total concentrations, and estrogenicity. This variability was evident in fish collected from the three rivers as no clear upstream to downstream pattern of endocrine disruption endpoints emerged.     

A river water quality model integrated with a web-based geographic information system

Scientists often use mathematical models to assess river water quality. However, the application of the models in environmental management and risk assessment is quite limited because of the difficulty of preparing input data and interpreting model output. This paper presents a study that links ArcIMS, a Web-based Geographic Information System (GIS) software to ROUT, a national and regional scale river model which evolved from the US Environmental Protection Agency's Water Use Improvement and Impairment Model, to create a WWW-GIS-based river simulation model called GIS-ROUT. GIS-ROUT is used to predict chemical concentrations in perennially flowing rivers throughout the continental United States that receive discharges from more than 10,000 publicly owned wastewater treatment plants (WWTPs). The WWTP chemical loadings are calculated from per capita per day disposal of product ingredients and the population served by each plant. Each WWTP, containing data on treatment type and influent and effluent flows, is spatially associated with a specific receiving river segment. Based on user defined treatment-type removal rates for a particular chemical, an effluent concentration for each WWTP is calculated and used as input to the river model. Over 360,000 km of rivers are modeled, incorporating dilution and first order loss of the chemical in each river segment. The integration of spatial data, GIS, the WWW, and modeling in GIS-ROUT makes it possible to organize and analyze data spatially, and view results on interactive maps as well as tables and distribution charts. The integration allows scientists and managers in different locations to coordinate and share their estimations for environmental exposure and risk assessments.     

Legacy Effects of Colonial Millponds on Floodplain Sedimentation,Bank Erosion,and Channel Morphology,Mid‐Atlantic,USA1

Abstract: Many rivers and streams of the Mid‐Atlantic Region, United States (U.S.) have been altered by postcolonial floodplain sedimentation (legacy sediment) associated with numerous milldams. Little Conestoga Creek, Pennsylvania, a tributary to the Susquehanna River and the Chesapeake Bay, is one of these streams. Floodplain sedimentation rates, bank erosion rates, and channel morphology were measured annually during 2004‐2007 at five sites along a 28‐km length of Little Conestoga Creek with nine colonial era milldams (one dam was still in place in 2007). This study was part of a larger cooperative effort to quantify floodplain sedimentation, bank erosion, and channel morphology in a high sediment yielding region of the Chesapeake Bay watershed. Data from the five sites were used to estimate the annual volume and mass of sediment stored on the floodplain and eroded from the banks for 14 segments along the 28‐km length of creek. A bank and floodplain reach based sediment budget (sediment budget) was constructed for the 28 km by summing the net volume of sediment deposited and eroded from each segment. Mean floodplain sedimentation rates for Little Conestoga Creek were variable, with erosion at one upstream site (?5 mm/year) to deposition at the other four sites (highest = 11 mm/year) despite over a meter of floodplain aggradation from postcolonial sedimentation. Mean bank erosion rates range between 29 and 163 mm/year among the five sites. Bank height increased 1 m for every 10.6 m of channel width, from upstream to downstream (R² = 0.79, p < 0.0001) resulting in progressively lowered hydraulic connectivity between the channel and the floodplain. Floodplain sedimentation and bank erosion rates also appear to be affected by the proximity of the segments to one existing milldam, which promotes deposition upstream and scouring downstream. The floodplain and bank along the 28‐km reach produced a net mean sediment loss of 5,634 Mg/year for 2004‐2007, indicating that bank erosion was exceeding floodplain sedimentation. In particular, the three segments between the existing dam and the confluence with the Conestoga River (32% of the studied reach) account for 97% of the measured net sediment budget. Future research directed at understanding channel equilibria should facilitate efforts to reduce the sediment impacts of dam removal and legacy sediment.     

WALNUT CREEK WATERSHED MONITORING PROJECT,IOWA MONITORING WATER QUALITY IN RESPONSE TO PRAIRIE RESTORATION1

ABSTRACT: Land use and surface water data for nitrogen and pesticides (1995 to 1997) are reported for the Walnut Creek Watershed Monitoring Project, Jasper County Iowa. The Walnut Creek project was established in 1995 as a nonpoint source monitoring program in relation to watershed habitat restoration and agricultural management changes implemented at the Neal Smith National Wildlife Refuge by the U.S. Fish and Wildlife Service. The monitoring project utilizes a paired‐watershed approach (Walnut and Squaw creeks) as well as upstream/downstream comparisons on Walnut for analysis and tracking of trends. From 1992 to 1997, 13.4 percent of the watershed was converted from row crop to native prairie in the Walnut Creek watershed. Including another 6 percent of watershed farmed on a cash‐rent basis, land use changes have been implemented on 19.4 percent of the watershed by the USFWS. Nitrogen and pesticide applications were reduced an estimated 18 percent and 28 percent in the watershed from land use changes. Atrazine was detected most often in surface water with frequencies of detection ranging from 76–86 percent. No significant differences were noted in atrazine concentrations between Walnut and Squaw Creek. Nitrate‐N concentrations measured in both watersheds were similar; both basins showed a similar pattern of detection and an overall reduction in nitrate‐N concentrations from upstream to downstream monitoring sites. Water quality improvements are suggested by nitrate‐N and chloride ratios less than one in the Walnut Creek watershed and low nitrate‐N concentrations measured in the subbasin of Walnut Creek containing the greatest amount of land use changes. Atrazine and nitrate‐N concentrations from the lower portion of the Walnut Creek watershed (including the prairie restoration area) may be decreasing in relation to the upstream untreated component of the watershed. The frequencies of pesticide detections and mean nitrate‐N concentrations appear related to the percentage of row crop in the basins and subbasins. Although some results are encouraging, definitive water quality improvements have not been observed during the first three years of monitoring. Possible reasons include: (1) more time is needed to adequately detect changes; (2) the size of the watershed is too large to detect improvements; (3) land use changes are not located in the area of the watershed where they would have greatest effect; or (4) water quality improvements have occurred but have been missed by the project monitoring design. Longer‐term monitoring will allow better evaluation of the impact of restoration activities on water quality.     

Occurrence of Primidone,Carbamazepine, Caffeine,and Precursors for N‐Nitrosodimethylamine in Drinking Water Sources Impacted by Wastewater1

Abstract: Wastewater impact on drinking water sources was assessed using several approaches, including analysis of three pharmaceuticals and personal care products (PPCPs) – primidone, carbamazepine, and caffeine – as indicators, and determination of precursor concentrations for the disinfection byproduct N‐nitrosodimethylamine (NDMA) using formation potential (FP) tests. Samples were collected in 2006 and 2007 in rivers impacted by wastewater treatment plant (WWTP) discharges, at drinking water treatment plant (DWTP) intakes upstream or downstream from these discharges, and from two WWTP effluents in two watersheds. The levels [10th percentile ? maximum (median)] of primidone, carbamazepine, caffeine, and NDMAFP were 2‐95 (7) ng/l, 2‐207 (18) ng/l, 7‐687 (78) ng/l, and 12‐321 (35) ng/l, respectively. The highest concentrations of primidone, carbamazepine, and NDMA precursors were from one of the WWTP effluents, whereas the highest concentration of caffeine was detected in a river heavily impacted by treated wastewater discharges. The lowest concentrations of the three PPCPs were from a DWTP influent upstream of a metropolitan urban area with minimum wastewater impact. Temporal variations in PPCP and NDMAFP concentrations and streamflows in two selected watersheds were also observed. Furthermore, correlation analysis between caffeine or carbamazepine and primidone was evaluated. The results show that measurement of the two pharmaceuticals and NDMAFP tests can be used to evaluate wastewater impact in different watersheds, whereas caffeine results were more variable.     

Biological Assessment of Aquaculture Effects on Effluent-Receiving Streams in Ghana Using Structural and Functional Composition of Fish and Macroinvertebrate Assemblages

Biological assessment of aquatic ecosystems is widely employed as an alternative or complement to chemical and toxicity testing due to numerous advantages of using biota to determine ecosystem condition. These advantages, especially to developing countries, include the relatively low cost and technical requirements. This study was conducted to determine the biological impacts of aquaculture operations on effluent-receiving streams in the Ashanti Region of Ghana. We collected water, fish and benthic macroinvertebrate samples from 12 aquaculture effluent-receiving streams upstream and downstream of fish farms and 12 reference streams between May and August of 2009, and then calculated structural and functional metrics for biotic assemblages. Fish species with non-guarding mode of reproduction were more abundant in reference streams than downstream (P?=?0.0214) and upstream (P?=?0.0251), and sand-detritus spawning fish were less predominant in reference stream than upstream (P?=?0.0222) and marginally less in downstream locations (P?=?0.0539). A possible subsidy-stress response of macroinvertebrate family richness and abundance was also observed, with nutrient (nitrogen) augmentation from aquaculture and other farming activities likely. Generally, there were no, or only marginal differences among locations downstream and upstream of fish farms and in reference streams in terms of several other biotic metrics considered. Therefore, the scale of impact in the future will depend not only on the management of nutrient augmentation from pond effluents, but also on the consideration of nutrient discharges from other industries like fruit and vegetable farming within the study area.     

Phosphorus concentrations, loads, and sources within the Illinois River drainage area, northwest Arkansas, 1997-2008

In the Ozark Highlands and across the United States, effluent phosphorus (P) sources often have a profound impact on water column concentrations and riverine transport. This study evaluated (i) annual P loads at the Illinois River at Arkansas Highway 59 from calendar year 1997 through 2008, (ii) the relative contribution of effluent P sources to annual riverine P transport, (iii) longitudinal gradients in water column P concentrations downstream from several wastewater treatment plant effluent discharges, and (iv) changes in monthly P loads over the last decade. This study showed that annual P loads have ranged from 64,000 kg to over 426,000 kg and that P transport was positively correlated to hydrology (i.e., the amount of water delivered downstream). The relative contribution of P inputs from municipal facilities has decreased from 40% of the annual P load at the Illinois River at Arkansas Highway 59 to < 15% in recent years. Elevated P concentrations during base flow conditions were traced 45 river km upstream to one municipal effluent discharge, but all effluent discharges influenced P concentrations in the receiving streams. Most important, flow-adjusted monthly P loads showed two distinct trends over time. Flow-adjusted loads significantly increased from 1997 through 2002 and significantly decreased from 2002 through 2008. The concentrations and transport of P within the Illinois River drainage area are significantly decreasing from all the watershed management changes that have occurred, and monitoring should continue to determine if this decrease continues at the same rate over the next several years.