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1.
The effects of mycorrhizal fungi and other soil microorganisms on growth of two grasses, Andropogon gerardii Vitm. and Festuca arundinacea Schreb., in heavy metal-contaminated soil and mine tailings were investigated. A. gerardii is highly dependent on mycorrhizal fungi in native prairie, while F. arundinacea is a facultative mycotroph and relies on mycorrhizal symbiosis only in extremely infertile soils. Regardless of microbial amendments, neither plant species was able to establish and grow in the mine tailings. Both plant species grew in the moderately contaminated or non-contaminated soils, although A. gerardii grew in these soils only when mycorrhizal. Other soil microbes significantly improved growth of A. gerardii only in uncontaminated soil, but to a lesser extent than mycorrhizae. Although F. arundinacea was more highly colonized by mycorrhizal fungi than A. gerardii, neither microbial amendment affected growth of fescue in any soil. In several treatments mycorrhizal fungi adapted to uncontaminated soil stimulated plant growth more than mycorrhizae adapted to the moderately contaminated soil. However, mycorrhizal fungi adapted to contaminated soil did not increase the productivity of plant growth in contaminated soil more than fungi adapted to uncontaminated soil. A. gerardii plants inoculated with mycorrhizal fungi retained more Zn in roots than in shoots, confirming earlier reports that mycorrhizal fungi alter the translocation pattern of heavy metals in host plants. In contrast, mycorrhizae did not affect translocation patterns in F. arundinaceae, suggesting that the mycorrhizal dependence of a plant species is correlated with the retention of metals in roots. The correlation between mycorrhizal dependence of a plant species and mycorrhizal alteration of translocation pattern may also explain the inconsistent reports of mycorrhizal effects on translocation of heavy metals in plants. Plant response to mycorrhizal symbiosis may therefore provide a useful criterion for the selection of the plant species to be used in revegetation of contaminated sites.  相似文献   

2.
Greenhouse experiments were conducted to examine the impact of expanded clay (Turface) or porous ceramic (Isolite) amendments on germination, biomass production, plant Zn concentration, and Zn accumulation by Festuca arundinacea grown in mine tailings. Because previous studies have demonstrated that fertilization is essential for plant growth in these tailings, manure was also added to the tailings. Plant growth and germination were greatest if the expanded clay was added topically to the tailings. To a lesser extent, plant growth and germination was also stimulated by topical additions of porous ceramic. However, no benefit was observed if either amendment was mixed into the top 10 cm of the mine tailings. The concentration of Zn in F. arundinacea tissues was lowest if the expanded clay was added topically to the mine tailings. Roots growing in the layer of clay or ceramic amendment appeared to be smaller, finer, and more abundant than the large, coarse roots found throughout the tailings-manure mixture. These results suggest that topical application of an expanded clay or porous ceramic product will increase seed germination and improve plant establishment and growth in contaminated minespoils.  相似文献   

3.
Biochars are biological residues combusted under low oxygen conditions, resulting in a porous, low density carbon rich material. Their large surface areas and cation exchange capacities, determined to a large extent by source materials and pyrolysis temperatures, enables enhanced sorption of both organic and inorganic contaminants to their surfaces, reducing pollutant mobility when amending contaminated soils. Liming effects or release of carbon into soil solution may increase arsenic mobility, whilst low capital but enhanced retention of plant nutrients can restrict revegetation on degraded soils amended only with biochars; the combination of composts, manures and other amendments with biochars could be their most effective deployment to soils requiring stabilisation by revegetation. Specific mechanisms of contaminant-biochar retention and release over time and the environmental impact of biochar amendments on soil organisms remain somewhat unclear but must be investigated to ensure that the management of environmental pollution coincides with ecological sustainability.  相似文献   

4.
In soils containing elevated levels of zinc, plant growth may be impaired because of Zn interference with P uptake by plants and because of detrimental effects of Zn toxicity itself. Because mycorrhizal fungi are known to improve uptake of plant P, the beneficial effects of mycorrhizal symbiosis on Zn tolerance of Andropogon gerardii Vitm. were assessed in soil amended with various levels of Zn and P. In the absence of P amendment, mycorrhizal fungi stimulated plant growth, but the degree of benefit depended on the inoculum source and the soil Zn level. Mycorrhizal fungi from a Zn contaminated site were more effective in increasing plant biomass at higher levels of Zn in the soil, whereas plant growth at lower levels of soil Zn was greater with mycorrhizal fungi from a non-contaminated site. Mycorrhizal fungus inoculation had no effect on shoot Zn concentration; however, inoculation significantly improved the plant P nutrition and therefore resulted in a high shoot P/Zn concentration ratio at all the soil Zn levels. To a certain extent, addition of P to the soil alleviated the Zn toxicity that had inhibited plant growth, but plant biomass tended to decrease with increasing soil Zn levels. Although P amendment improved P uptake, it also resulted in increased shoot Zn uptake.  相似文献   

5.
Zn accumulation in Solanum nigrum grown in naturally contaminated soil in the presence of different types of organic amendments was assessed. Under the same conditions, the response of the plant to inoculation with two different isolates of arbuscular mycorrhizal fungi (AMF) (Glomus claroideum and Glomus intraradices) was also evaluated. S. nigrum grown in the non-amended soil always presented higher Zn accumulation in the tissues, with the addition of amendments inducing reductions of up to 80 and 40%, for manure and compost, respectively, and enhancing plant biomass yields. The establishment of S. nigrum in the Zn contaminated soil combined with the application of amendments led to a 70-80% reduction in the amount of Zn leached through the soil. The use of S. nigrum in combination with manure appeared as an effective method for reducing the effects of soil contamination, diminishing Zn transfer to other environmental compartments via percolation.  相似文献   

6.
Aided phytostabilization is a technology that uses metal tolerant plants and organic and/or inorganic amendments to reduce soil metal bioavailability, while improving soil health. Our objective was to determine the effects of the application of amendments [sheep manure (SHEEP), poultry litter (POULTRY), cow slurry (COW), and paper mill sludge mixed with poultry litter (PAPER)], together with the growth of a metallicolous Festuca rubra L. population, on (i) chemical and microbial indicators of soil health and (ii) soil ecotoxicity, during the aided phytostabilization of a Zn/Pb contaminated mine soil. Amendment application led to an increase in soil pH, organic matter content, and inorganic salts, resulting in a decrease in Pb and Zn CaCl2-extractable concentrations in soil, which, in turn, contributed to lower ecotoxicity and a stimulation of plant growth and soil microbial communities. The factor most affecting the metal extractability was probably soil pH. POULTRY was the best amendment in terms of increasing plant growth, chlorophylls content, and soil microbial biomass and activity, but resulted in higher levels of phytoavailable Pb and Zn. SHEEP and PAPER were more effective at reducing metal CaCl2-extractability and, consequently, led to lower values of metal accumulation in plant tissues, thereby reducing the risk of metals entering into the food chain. When combined with the application of organic amendments, the metallicolous F. rubra population studied here appears an excellent candidate for aided phytostabilization. Our results indicate that the application of organic amendments is essential for the short-term recovery of highly contaminated metalliferous soils during aided phytostabilization.  相似文献   

7.
随着工业和经济的发展,土壤重金属污染越来越严重,因此重金属污染土壤的修复迫在眉睫。植物修复技术因其经济、环保等优点已经成为国内外研究的热点,但存在修复植物稀少的问题。将其他技术与植物修复技术联用可能是突破植物修复这一局限性的关键举措之一。介绍了基因工程技术与植物修复技术联用的研究进展;总结了微生物技术与植物修复技术联用中植物根际促生细菌、菌根真菌的作用;概述了材料化学技术联合植物修复的应用现状,着重介绍了土壤改良剂和螯合剂的实际应用。最后对基因工程、微生物、材料化学等技术联合植物修复技术在研究和应用中的未来方向进行了前景展望,旨在为植物修复技术发展提供更多新的思路和参考。  相似文献   

8.
We tested the effects of three amendments (a biosolid compost, a sugar beet lime, and a combination of leonardite plus sugar beet lime) on trace element stabilisation and spontaneous revegetation of a trace element contaminated soil. Soil properties were analysed before and after amendment application. Spontaneous vegetation growing on the experimental plot was studied by three surveys in terms of number of taxa colonising, percentage vegetation cover and plant biomass. Macronutrients and trace element concentrations of the five most frequent species were analysed. The results showed a positive effect of the amendments both on soil chemical properties and vegetation. All amendments increased soil pH and TOC content and reduced CaCl(2)-soluble-trace element concentrations. Colonisation by wild plants was enhanced in all amended treatments. The nutritional status of the five species studied was improved in some cases, while a general reduction in trace element concentrations of the aboveground parts was observed in all treated plots. The results obtained show that natural assisted remediation has potential for success on a field scale reducing trace element entry in the food chain.  相似文献   

9.
We investigated the relationship of the zonal pattern followed by the vegetation in a polluted Mediterranean salt marsh, in semiarid south-eastern Spain, with the microbiological and biochemical properties (labile C fractions, oxidoreductases and hydrolases) of the rhizosphere soil of two halophyte species, Arthrocnemum macrostachyum and Sarcocornia fruticosa, and with the degree of arbuscular mycorrhizal (AM) colonisation in their rhizospheres. Levels of plant biomass and cover were inversely related to heavy metal contents and salinity. The concentrations of Fe, Cu, Mn and Pb extracted with DTPA hardly varied among the different zones of the salt marsh. The dehydrogenase and phosphatase activities, the soluble C and water-soluble carbohydrates concentrations and the extent of root colonisation were greater in the salt marsh zones of lower soil salinity and lower metal concentration. Urease and beta-glucosidase activities were not detected in the salt marsh. Plant biomass and cover showed positive relationships with mycorrhizal colonisation (R=0.773, P<0.001; R=0.874, P<0.001, respectively). Mycorrhizal colonisation was negatively correlated with the contents of Pb and Zn in plant tissues. This work supports the view that reduced plant uptake of toxic metals, particularly lead, could be involved in the beneficial effects of AM fungi on plant development in Mediterranean salt marshes contaminated with mining wastes.  相似文献   

10.
A 6.6 ha grassland, established on a former chemical waste site adjacent to a residential area, contains arsenic (As) in surface soil at concentrations 200 times higher than UK Soil Guideline Values. The site is not recognized as statutory contaminated land, partly on the assumption that mobility of the metalloid presents a negligible threat to human health, groundwater and ecological receptors. Evidence for this is evaluated, based on studies of the effect of organic (green waste compost) and inorganic (iron oxides, lime and phosphate) amendments on As fractionation, mobility, plant uptake and earthworm communities. Arsenic mobility in soil was low but significantly related to dissolved organic matter and phosphate, with immobilization associated with iron oxides. Plant uptake was low and there was little apparent impact on earthworms. The existing vegetation cover reduces re-entrainment of dust-blown particulates and pathways of As exposure via this route. Minimizing risks to receptors requires avoidance of soil exposure, and no compost or phosphate application.  相似文献   

11.
Field trials concerning the establishment of plant cover on a deposit of wastes from the Ammeberg zinc mine in central Sweden were carried out during 1976-1985. Different soil conditioners and manures were applied and plant species cultivars were evaluated with regard to plant biomass, vigour, durability and content of zinc, lead and cadmium. Sewage sludge and topsoil led to better establishment of grasses than did municipal waste, straw and hydraulic seeding. After 2 years, Festuca rubra and Poa pratensis dominated the swards. Other species (Dactylis glomerata, Bromus inermis, Lolium perenne, Phleum nodosum, Festuca pratensis and F. arundinacea) constituted only a minor part of the stand. After 10 years, F. rubra was the most dominant species, while native Agrostis tenuis had invaded 20-50% of the area within the plots. Merlin was the clearly dominant red fescue cultivar. The concentration of zinc in shoots (616 mg kg(-1) dw) was about 10% of that in the soil. Zinc concentration decreased with increasing biomass above ground. It increased with age in Scots pine needles and was very high in birch leaves. Grasses survived longer than legumes in the zinc sand waste. Among the surviving grasses was a group with high (3800 mg kg(-1) dw) and a group with low (320 mg kg(-1) dw) zinc concentrations. The low group included Merlin red fescue and Sobel creeping bent. The cultivar Merlin contained a much lower zinc concentration than the other cultivars of red fescue (375 and 624 mg kg(-1) dw, respectively). A large amount of root biomass was present in plots with dominating Merlin red fescue (1715 g m(-2)), 97% of which was concentrated in the top 10 cm of the soil. The concentration of zinc in the roots was very high (13 000-25 000 mg kg(-1) dw). Nitrate fertilizer, especially ammonium nitrate, and acidic water (pH 4.3) increased zinc leaching.  相似文献   

12.
Several wetland plant species appear to have constitutive metal tolerance. In previous studies, populations from contaminated and non-contaminated sites of the wetland plants Typha latifolia, Phragmites australis, Glyceria fluitans and Eriophorum angustifolium were found to be tolerant to high concentrations of metals. This study screened three other species of wetland plants: Alisma plantago-aquatica, Carex rostrata and Phalaris arundinacea for innate tolerance to zinc. The degree of tolerance was compared to known zinc-tolerant E. angustifolium and Festuca rubra Merlin. It was found that A. plantago-aquatica and P. arundinacea did not posses innate tolerance to zinc, but that C. rostrata was able to tolerate elevated levels of zinc, at levels comparable to those tolerated by E. angustifolium and F. rubra Merlin. The findings support the theory that some wetland angiosperm species tend to be tolerant to exposure to high levels of metals, regardless of their origin.  相似文献   

13.
A nursery experiment was conducted to evaluate the potential role of arbuscular mycorrhizal (AM) fungi in encouraging the vegetation cover on bauxite residue (red mud) sites. An alkali tolerant bermudagrass (Cynodon dactylon) adapted to local conditions were grown in red mud with different amendments with and without AM fungi to assess mycorrhizal effects on plant growth, mineral nutrition, metal uptake and neutralization of bauxite residue. Inoculation of AM fungi significantly increased the plant growth, nutrient uptake and reduced Fe, Al accumulation in plant tissue and also improved the soil physico-chemical and biochemical properties. Gypsum and sludge amended treatments inoculated with AM fungi had maximum biomass, nutrient uptake and reduced accumulation of metals. The neutralization of red mud was significant in presence of AM fungi than control. The experiment provided evidence for the potential use of bermudagrass in combination with AM fungi for ecological restoration of bauxite residue sites.  相似文献   

14.
Background, aim, and scope  Tobacco, Nicotiana tabacum, is a widely used model plant for growth on heavy-metal-contaminated sites. Its high biomass and deep rooting system make it interesting for phytoextraction. In the present study, we investigated the antioxidative activities and glutathione-dependent enzymes of different tobacco clones optimized for better Cd and Zn accumulation in order to characterize their performance in the field. Main features  The improved heavy metal resistance also makes the investigated tobacco clones interesting for understanding the plant defense enzyme system in general. Freshly harvested plant material (N. tabacum leaves) was used to investigate the antioxidative cascade in plants grown on heavy metal contaminated sites with and without amendments of different ammonium nitrate and ammonium sulfate fertilizers. Materials and methods  Plants were grown on heavily polluted soils in north-east Switzerland. Leaves were harvested at the field site and directly deep frozen in liquid N2. Studies were concentrated on the antioxidative enzymes of the Halliwell–Asada cycle, and spectrophotometric measurements of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), superoxide dismutase (SOD, EC 1.15.1.1), glutathione peroxidase (GPX, EC 1.11.1.9), glutathione reductase (GR, EC 1.6.4.2), glutathione S-transferase (GST, EC 2.5.1.18) were performed. Results and discussion  We tried to explain the relationship between fertilizer amendments and the activity of the enzymatic defense systems. When tobacco (N. tabacum) plants originating from different mutants were grown under field conditions with varying fertilizer application, the uptake of cadmium and zinc from soil increased with increasing biomass. Depending on Cd and Zn uptake, several antioxidant enzymes showed significantly different activities. Whereas SOD and CAT were usually elevated, several other enzymes, and isoforms of GST were strongly inhibited. Conclusions  Heavy metal uptake represents severe stress to plants, and specific antioxidative enzymes are induced at the cost of more general reactions of the Halliwell–Asada cycle. In well-supplied plants, the glutathione level remains more or less unchanged. The lack of certain glutathione S-transferases upon exposure to heavy metals might be problematic in cases when organic pollutants coincide with heavy metal pollution. When planning phytoremediation of sites, mixed pollution scenarios have to be foreseen and plants should be selected according to both, their stress resistance and hyperaccumulative capacity.  相似文献   

15.
Dahiya S  Shanwal AV  Hegde AG 《Chemosphere》2005,60(9):1253-1261
Zinc adsorption was studied in the soils of three nuclear power plant sites of India. 65Zn was used as a radiotracer to study the sorption characteristics of Zn(II). The sorption of zinc was determined at 25 and 45 degrees C at pH 7.8+/-0.2 in the solution of 0.01 M Ca(NO3)2 as supporting electrolyte. The sorption data was tested both in Freundlich and Langmuir isotherms and could be described satisfactorily. The effect of organic matter and other physico-chemical properties on the uptake of zinc was also studied in all the soil samples. The results showed that the cation exchange capacity, organic matter, pH and clay content were the main contributors to zinc sorption in these soils. The adsorption maximum was found to be higher in the soil on Kakarpara Atomic Power Plant sites soils having high organic matter and clay content. The zinc supply parameters of the soils are also discussed. In the desorption studies, the sequential extraction of the adsorbed zinc from soils showed that the diethylene triamine penta acetic acid extracted maximum amount of adsorbed zinc than CaCl2 and Mg(NO3)2. The zinc sorption on the soil and amount of zinc retention after extractants desorption shows a positively correlation with vermiculite and smectite mineral content present in the clay fraction of the soil. The amount desorbed by strong base (NaOH) and demineralised water was almost negligible from soils of all the sites, whereas the desorption by strong acid (HNO3) was 75-96% of the adsorbed zinc.  相似文献   

16.
The applicability of a mature compost as a soil amendment to promote the growth of native species for the phytorestoration of a mine-affected soil from a semi-arid area (SE Spain), contaminated with trace elements (As, Cd, Cu, Mn, Pb and Zn), was evaluated in a 2-year field experiment. The effects of an inorganic fertiliser were also determined for comparison. Bituminaria bituminosa was the selected native plant since it is a leguminous species adapted to the particular local pedoclimatic conditions. Compost addition increased total organic-C concentrations in soil with respect to the control and fertiliser treatments, maintained elevated available P concentrations throughout the duration of the experiment and stimulated soil microbial biomass, while trace elements extractability in the soil was rather low due to the calcareous nature of the soil and almost unaltered in the different treatments. Tissue concentrations of P and K in B. bituminosa increased after the addition of compost, associated with growth stimulation. Leaf Cu concentration was also increased by the amendments, although overall the trace elements concentrations can be considered non-toxic. In addition, the spontaneous colonisation of the plots by a total of 29 species of 15 different families at the end of the experiment produced a greater vegetation cover, especially in plots amended with compost. Therefore, the use of compost as a soil amendment appears to be useful for the promotion of a vegetation cover and the phytostabilisation of moderately contaminated soils under semi-arid conditions.  相似文献   

17.
This study evaluated the effect of alkaline industrial by-products such as flyash (FA) and redmud (RM) on phosphorus (P) mobilisation in abattoir wastewater irrigated soils, using incubation, leaching and plant growth (Napier grass [Pennisetum purpureum]) experiments. The soil outside the wastewater irrigated area was also collected and treated with inorganic (KH2PO4 [PP]) and organic (poultry manure [PM]) P treatments, to study the effect of FA and RM on P mobilisation using plant growth experiment. Among the amendments, FA showed the highest increase in Olsen P, oxalic acid content and phosphatase activity. The highest increase in Olsen P for PM treated non-irrigated soils showed the ability of FA and RM in mobilising organic P better than inorganic P (PP). There was over 85 % increase in oxalic acid content in the plant growth soils compared to the incubated soil, showing the effect of Napier grass in the exudation of oxalic acid. Both amendments (FA and RM) showed an increase in phosphatase activity at over 90 % at the end of the 5-week incubation period. The leaching experiment indicated a decrease in water soluble P thereby ensuring the role of FA and RM in minimising P loss to water bodies. FA and RM showed an increase in plant biomass for all treatments, where FA amended soil showed the highest increase as evident from FA’s effect on Olsen P. Therefore, the use of FA and RM mobilised P in abattoir wastewater irrigated soils and increased biomass production of Napier grass plants through root exudation of oxalic acid.  相似文献   

18.
Metal tolerance and phytoextraction potential of two common sorrel (Rumex acetosa L.) accessions, collected from a Pb/Zn contaminated site (CS, Lanestosa) and an uncontaminated site (UCS, Larrauri), were studied in fertilized and non-fertilized pots prepared by combining soil samples from both sites in different proportions (i.e., 0%, 33%, 66% and 100% of Lanestosa contaminated soil). The original metalliferous mine soil contained 20480, 4950 and 14 mg kg(-1) of Zn, Pb and Cd, respectively. The microcosm experiment was carried out for two months under greenhouse controlled conditions. It was found that fertilization increased mean plant biomass of both accessions as well as their tolerance. However, only the CS accession survived all treatments even though its biomass decreased proportionally according to the percentage of contaminated mine soil present in the pots. This metallicolous accession would be useful for the revegetation and phytostabilization of mine soils. Due to its high concentration and bioavailability in the contaminated soil, the highest values of metal phytoextracted corresponded to Zn. The CS accession was capable of efficiently phytoextracting metal from the 100% mine soil, indeed reaching very promising phytoextraction rates in the fertilized pots (6.8 mg plant(-1) month(-1)), similar to the ones obtained with hyperaccumulator plants. It was concluded that fertilization is certainly worth being considered for phytoextraction and revegetation with native plants from metalliferous soils.  相似文献   

19.
Plant communities on plots with different metal pollution levels were compared in a field study in order to select the most suitable plant species for the direction of secondary succession toward the targeted grassland vegetation. The vegetational succession showed a gradual increase in plant cover and the number of plant species on the less polluted locations. Two predominant grass species Calamagrostis varia and Sesleria caerulea were selected for phytostabilisation, but a severely reduced seed germination capacity obstructed their use in practice. The mycorrhizal succession showed a gradual replacement of non-mycorrhizal with mycorrhizal plant species. Similar levels of arbuscular mycorrhizal colonisation of a particular plant species may be developed within each growing season regardless of the levels of pollution, with the exception of vesicle/intraradical spore formation. The results suggest that lower overall mycorrhizal colonisation levels and increased vesicle/spore formation may be a part of a mycorrhizal strategy at the most polluted locations.  相似文献   

20.
Due to anthropogenic activities, large extends of soils are highly contaminated by Metal Trace Element (MTE). Aided phytostabilisation aims to establish a vegetation cover in order to promote in situ immobilisation of trace elements by combining the use of metal-tolerant plants and inexpensive mineral or organic soil amendments. Eight years after Coal Fly Ash (CFA) soil amendment, MTE bioavailability and uptake by two plants, Lolium perenne and Trifolium repens, were evaluated, as some biological markers reflecting physiological stress. Results showed that the two plant species under study were suitable to reduce the mobility and the availability of these elements. Moreover, the plant growth was better on CFA amended MTE-contaminated soils, and the plant sensitivity to MTE-induced physiological stress, as studied through photosynthetic pigment contents and oxidative damage was lower or similar. In conclusion, these results supported the usefulness of aided phytostabilisation of MTE-highly contaminated soils.  相似文献   

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