Natural and man-made organobromine compounds in marine biota from Central Norway

Brominated organic pollutants were found in selected samples of mollusk tissue, fish liver, as well as in the eggs and livers of shag from three sites in Central Norway. More than 80 organobromines were identified owing to the defined isotope ratio acquired by GC/ECNI-MS. However, only a few peaks could be assigned to anthropogenic brominated flame retardants (polybrominated diphenyl ethers). Most of the organobromine compounds detected were unknown or halogenated natural products. The known halogenated natural products MHC-1 and TBA were abundant in all samples and usually between equally abundant, and up to 50 fold more concentrated than the major polybrominated diphenyl ether congener BDE 47. The halogenated natural products BC-2 (2-MeO-BDE 68) and BC-3 (6'-MeO-BDE 47), were on level with BDE 100 which was the second most abundant BDE congener in many samples. The previously identified natural polybrominated hexahydroxanthene derivatives (PBHDs) were detected for the first time in bird eggs. Being major contaminants in bird eggs, PBHDs were only present at low levels in bird liver from nestlings originating from the same clutch. Such differences were detected for several other major contaminants. One unknown tetrabromo compound particularly abundant in mussels from Munkholmen was studied by GC/MS and the molecular ion was detected at m/z 446. The abundance of the most important unknown compounds did not correlate with BDEs and they most likely represent halogenated natural products. This study supports that halogenated natural products have to be treated as serious contaminants of marine coastal waters. Our data suggest that their abundance is highest in habitats along the shoreline. Thorough examination of these compounds in environmental samples is an important task.     

Variations of 210Po and 210Pb in various marine organisms from Western English Channel: contribution of 210Po to the radiation dose

Measurements of (210)Po were carried out in various marine matrices (mussels, oysters, seaweed, fish, and abalones) and in seawater at several points along the French coast, over a period of 2 years (2003-2005). These measurements contribute to a better knowledge of this element, since few recent data exist for the French coast. Marked seasonal variations have been revealed in some species and there are differences according to the way of life of these species. Activities in mussels (Mytilus edulis) and oysters (Crassostrea gigas) are similar and varying between 90 and 600 Bq kg(-1) (d.w.). Activities in macroalgae (Fucus serratus) are lowest, between 4 and 16 Bq kg(-1) (d.w.). In oyster, abalone (Haliotis tuberculata) and fish (Solea solea, Sparus sp.), the strongest activities are measured in the digestive glands, the gills and the gonads. (210)Po/(210)Pb ratios in all cases have values of more than one for all species. From a significant number of measurements, CFs were calculated for seaweed (between 4.6 x 10(3) and 5.0 x 10(3)) and for molluscs, with highest CFs (>10(5)) found for the digestive gland and gills of the oysters, the digestive gland of the abalones and the liver of fish. Finally, the activities measured have made it possible to estimate the internal dose from chronic exposure due to (210)Po received by the marine organisms (0.05 microGh(-1) for macroalgae, between 0.70 and 1.5 microGh(-1) for mussels and oyster), and the contribution of seafood to the dose received by humans (46-129 microSvy(-1)).     

Perfluoroalkyl compounds in Danish wastewater treatment plants and aquatic environments

This study reports the results of a screening survey of perfluoroalkyl compounds (PFCs) in the Danish environment. The study included point sources (municipal and industrial wastewater treatment plants and landfill sites) and the marine and freshwater environments. Effluent and influent water and sewage sludge were analysed for point sources. Sediment, blue mussels (Mytilus edulis) and liver from plaice (Pleuronectes platessa), flounder (Platichthys flesus) and eel (Anguilla anguilla) were analysed for the freshwater and marine environments. The results obtained show a diffuse PFCs contamination of the Danish environment with concentrations similar to those measured in other countries with the absence of primary contamination sources such as fluorochemical production. PFOS and PFOA were generally the most dominating PFCs measured in both point sources and the aquatic environments. PFCs were found in both inflow and outflow water and sewage sludge from municipal and industrial wastewater treatment plants (WWTPs), indicating that WWTPs can be significant sources to PFCs in the environment. This is also reflected in the locally elevated PFCs concentrations found in fish like eels from shallow freshwater and marine areas. However, the highest PFCs concentrations found in fish in this study was in plaice from the Skagerrak (156 ng/g wet weight PFOS), but it is unknown if this can be related to significant sources in the North Sea region or to differences between species. The concentrations of PFCs were below the detection limit in all analysed freshwater and marine samples of sediment and mussels. Despite the relatively low PFCs concentrations measured in marine fish, the high bioaccumulation potential of PFCs, particularly PFOS, may lead to high concentrations of PFCs in marine mammals as shown by previous investigations.     

汉江下游鱼类早期资源及梯级联合生态调度对鱼类繁殖影响的初步分析

汉江是长江一级支流,鱼类资源较为丰富。目前汉江中下游建有4座水利枢纽工程,对鱼类洄游和繁殖产生较大影响。在鱼类繁殖季节,为促进汉江中下游鱼类繁殖,2018年6月汉江中下游首次实施梯级联合生态调度。该研究在汉江下游汉川段设置监测断面,采集鱼类早期资源,以分析汉江下游鱼类早期资源多样性,及生态调度对促进汉江下游鱼类繁殖的效果。监测结果表明汉江下游卵苗种类共有36种（属）,其中22种为产漂流性卵鱼类。监测期间卵苗总径流量为118.78亿尾（粒）,其中四大家鱼卵苗为0.87亿尾（粒）,占0.73%,其中鱼卵0.69亿粒,推测汉江下游有4处四大家鱼产卵场。梯级联合生态调度期间,汉川江段出现一次鱼类产卵高峰,鱼卵径流量占总径流量的34.1%,其中四大家鱼鱼卵占家鱼卵总径流量的85.5%,表明梯级联合生态调度对汉江下游鱼类,特别是四大家鱼的繁殖具有积极的作用。建议今后继续开展生态调度,并加强监测和分析工作。     

Determination of toxic elements (mercury,cadmium, lead,tin and arsenic) in fish and shellfish samples. Risk assessment for the consumers

Although fish intake has potential health benefits, the presence of metal contamination in seafood has raised public health concerns. In this study, levels of mercury, cadmium, lead, tin and arsenic have been determined in fresh, canned and frozen fish and shellfish products and compared with the maximum levels currently in force. In a further step, potential human health risks for the consumers were assessed. A total of 485 samples of the 43 most frequently consumed fish and shellfish species in Andalusia (Southern Spain) were analyzed for their toxic elements content. High mercury concentrations were found in some predatory species (blue shark, cat shark, swordfish and tuna), although they were below the regulatory maximum levels. In the case of cadmium, bivalve mollusks such as canned clams and mussels presented higher concentrations than fish, but almost none of the samples analyzed exceeded the maximum levels. Lead concentrations were almost negligible with the exception of frozen common sole, which showed median levels above the legal limit. Tin levels in canned products were far below the maximum regulatory limit, indicating that no significant tin was transferred from the can. Arsenic concentrations were higher in crustaceans such as fresh and frozen shrimps. The risk assessment performed indicated that fish and shellfish products were safe for the average consumer, although a potential risk cannot be dismissed for regular or excessive consumers of particular fish species, such as tuna, swordfish, blue shark and cat shark (for mercury) and common sole (for lead).     

Changes in the structure of ichthyofauna in water bodies of Kaluga oblast over the past few decades

The structure of the ichthyofauna in water bodies of Kaluga oblast has undergone significant rearrangements over the past 20 years. The abundance of some species has increased, while that of other species has decreased; spontaneous dispersal of some fishes is underway. Synchronous fluctuations of fish abundance (“waves of life”) take place simultaneously in the Western Dvina, Dnieper, and Volga basins. These processes differ in intensity depending on fish species and are conditioned by a number of factors, including changes in hydrological regimes of rivers, improvement of the ecological state of small rivers, and increased poaching pressure. The results of monitoring the ichthyofauna fauna of small rivers are used as an example to trace the routes of fish dispersal and discuss changes in the composition of fish communities.     

Fish Communities of Small Ponds in the Udmurt Republic: Structural Features and Trends in Anthropogenic Successions

The ichthyofauna of 20 small ponds in the Udmurt Republic was studied to estimate the dependence of fish species abundance on pond size and anthropogenic load, as well as trends in the succession of dominant species complexes in ponds exposed to the prevailing impact of agricultural activity. Changes in the ecological structure of fish communities in small ponds upon an increase in the anthropogenic load were analyzed. Some specific features of the species composition of ichthyofauna in technogenic pond biocenoses were revealed.     

Measured and modelled tritium concentrations in freshwater Barnes mussels (Elliptio complanata) exposed to an abrupt increase in ambient tritium levels

To improve understanding of environmental tritium behaviour, the International Atomic Energy Agency (IAEA) included a Tritium and C-14 Working Group (WG) in its EMRAS (Environmental Modelling for Radiation Safety) program. One scenario considered by the WG involved the prediction of time-dependent tritium concentrations in freshwater mussels that were subjected to an abrupt increase in ambient tritium levels. The experimental data used in the scenario were obtained from a study in which freshwater Barnes mussels (Elliptio complanata) were transplanted from an area with background tritium concentrations to a small Canadian Shield lake that contains elevated tritium. The mussels were then sampled over 88 days, and concentrations of free-water tritium (HTO) and organically-bound tritium (OBT) were measured in the soft tissues to follow the build-up of tritium in the mussels over time.The HTO concentration in the mussels reached steady state with the concentration in lake water within one or two hours. Most models predicted a longer time (up to a few days) to equilibrium. All models under-predicted the OBT concentration in the mussels one hour after transplantation, but over-predicted the rate of OBT formation over the next 24 h. Subsequent dynamics were not well modelled, although all participants predicted OBT concentrations that were within a factor of three of the observation at the end of the study period. The concentration at the final time point was over-predicted by all but one of the models. The relatively low observed concentration at this time was likely due to the loss of OBT by mussels during reproduction.     

三峡水库蓄水后长江中游宜昌江段鱼类早期资源群聚动态

基于2007～2018年葛洲坝下宜昌江段鱼类早期资源调查资料的整理分析,采用群落生态学方法对12年来长江中游鱼类早期资源群聚特性和年际变化趋势进行了研究,探讨了三峡水库蓄水后鱼类早期资源的优势种更替过程和群落结构变异.结果显示:12年间共采集到鱼卵5目9科49种,其中产漂流性卵鱼类28种.鱼卵物种数的年际变化趋势不显著,但年间变幅较大(18～30种/a).鱼卵丰度的年际变化表现出明显的趋势性上升,其中"四大家鱼"采集数量和密度呈先下降后上升的"U"型变化趋势.群落结构分析显示,12年间宜昌江段鱼卵物种多样性指数均呈缓慢下降趋势,并在2007～2008、2009～2013和2014～2018年形成三组不同的时间聚类,组间差异显著.SIMPER分析表明,各时期鱼卵群落差异主要受优势种和重要种所决定.2013年前,贝氏(鳖)(Hemiculter.bleekeri)、银鮑(Squalidus argentatus)、银鲴(Xerocypris macrolepis)、草鱼(Ctenopha-ryngodonidellus)等交替出现成为主要优势物种;2013年后,贝氏(鳖)成为主要优势物种,优势种和重要种的鱼卵数量占比进一步上升(大于90％).研究结果指出,2003年三峡水库蓄水后,葛洲坝下宜昌江段鱼类早期资源优势种更替现象逐渐消失,群落结构趋于稳定,繁殖鱼类群落结构正向"单一化"发展.     

巢湖流域河流鱼类群落的时空分布

基于2013年4月和10月对巢湖流域66个河道样点的调查数据,初步研究了巢湖流域河流鱼类群落的时空变化特征。主要研究结果显示,鱼类多样性无显著性的水系间、生态分区间的变化,但随季节和河流级别显著变化:10月份的个体数显著高于4月份,2级河流的物种数和个体数均显著大于1级、3级和4级河流。鱼类群落结构的季节动态显著,随生态分区显著变化,但不受水系、河流级别的显著影响。在二级生态分区水平上,仅西南森林生态亚区的鱼类群落结构与其他5个生态亚区的显著差异;宽鳍鱲、吻虾虎鱼等在西南森林生态亚区具有更高多度,而鲫、鰐、鲤等物种在其他生态亚区多度更高。     

洞庭湖通江水道鱼类资源周年动态及其洄游特征研究

为了解洞庭湖通江水道鱼类资源的周年动态及其洄游特征,2017年5月～2018年6月,对洞庭湖通江水道的仔鱼和幼鱼、成鱼进行了逐月调查。(1)共采集仔鱼19种,数量以■(Hemiculter leucisculus)最多(占总数的76. 6%),其次为子陵吻虾虎鱼(Rhinogobius giurinus)(占8. 8%)和似鳊(Pseudobrama simoni)(占4. 3%);仔鱼主要出现在4～9月,密度以6月最高(848. 8 Ind./1 000 m~3)。(2)采集幼鱼、成鱼42种,数量以短颌鲚(Coilia brachygnathus)(占总数的30. 3%)最多,其次为贝氏■(Hemiculter bleekeri)(占15. 3%)和■(占12. 2%);幼鱼、成鱼丰度以8月最高(为729尾/船/日)。(3) 4～7月,江湖洄游型鱼类的运动指数在0. 1～0. 7之间,呈现入湖趋势; 9～次年3月,江湖洄游型鱼类的运动指数在-0. 4～-0. 1之间,呈现出湖趋势;径流量是幼鱼、成鱼出、入湖的主要驱动因子。     

Effect of habitat conditions on proteinase and glycosidase activities in the gut microbiota of crucian carp in a wide range of pH

Significant differences in pH dependence of proteolytic and amylolytic enzyme activities in the gut microbiota between crucian carps from different habitats (the Dniester River and Kuchurgan Reservoir) have been revealed for the first time. The proteolytic activity in fish from these water bodies reaches a peak at pH values varying from 7 to 10, while the peak of amylolytic activity is consistently observed at pH 7.0. Both proteolytic and amylolytic activities are higher in fish from the Kuchurgan Reservoir (characterized by increased trophicity and high abundance of bacterioplankton) than in fish from the Dniester. For comparison, the effect of pH on proteinase and glycosidase activities in the gut mucosa and chyme of the same fish has been studied.     

Evidence of genetic damage in grass gobies and mussels from the Venice lagoon

The presence of genetic damage has been investigated in two native species of the Venice lagoon: the common mussel Mytilus galloprovincialis and the grass goby Zosterisessor ophiocephalus. Two sampling campaigns were performed in summer 1998 and 1999. Aromatic-like DNA adducts were analysed in selected tissues of gobies and mussels by using the 32P-postlabelling assay. In 1999, micronuclei and other nuclear abnormalities were additionally scored on gill cells and haemocytes of individual mussels whereas inorganic (As, Cd, Cr, Hg, Ni, Pb, Sn) as well as organic contaminants (polycyclic aromatic hydrocarbons, polychlorinated biphenyls and other chlorinated compounds) were measured in the total mussel pulp. Compared to the lagoon inlet area, gobies and mussels from the industrial district (Marghera) showed significant DNA adduct levels and increased frequencies of cytogenetic alterations (evidence of genetic damage was absent or inconsistent in other sites). The substantial levels of aromatic and chlorinated contaminants detected in mussels from Marghera also support the exposure of native organisms to genotoxic agents.     

Technetium-99 in the Irish marine environment

Technetium-99 activity concentrations in seawater and biota from Irish coastal waters are presented. Time series measurements of 99Tc in seawater and Fucus vesiculosus from the western Irish Sea show that activity concentrations have increased in line with the increase in discharges of 99Tc from Sellafield. The peak in activity concentrations in both seawater and Fucus vesiculosus occurred in 1997 approximately two years after the peak in 99Tc discharges. The highest activity concentration recorded in Fucus vesiculosus showed a 29-fold increase over the mean concentration for the period 1988-1993. Technetium-99 activity concentrations were measured in fish, lobsters, prawns, mussels and oysters landed at major fishing ports on the east and northeast coasts of Ireland between 1996 and 1998. Concentration factors for 99Tc in the brown seaweed Fucus vesiculosus and certain species of fish, crustaceans and molluscs from the Irish Sea were estimated. In general, these concentration factors were higher than those in the literature which were derived from laboratory studies, but agreed well with values which were based on field studies. The mean committed effective doses to Irish typical and heavy seafood consumers due to 99Tc in the period 1996-1998 were 0.061 and 0.24 microSv, respectively.     

A study of radium bioaccumulation in freshwater mussels, Velesunio angasi, in the Magela Creek catchment, Northern Territory, Australia

Freshwater mussels, Velesunio angasi, along Magela Creek in Australia’s Northern Territory were examined to study radionuclide activities in mussel flesh and to investigate whether the Ranger Uranium mine is contributing to the radium loads in mussels downstream of the mine. Radium loads in mussels of the same age were highest in Bowerbird Billabong, located 20 km upstream of the mine site. Variations in the ratio of [Ra]:[Ca] in filtered water at the sampling sites accounted for the variations found in mussel radium loads with natural increases in calcium (Ca) in surface waters in a downstream gradient along the Magela Creek catchment gradually reducing radium uptake in mussels. At Mudginberri Billabong, 12 km downstream of the mine, concentration factors for radium have not significantly changed over the past 25 years since the mine commenced operations and this, coupled with a gradual decrease of the ²²⁸Ra/²²⁶Ra activity ratios observed along the catchment, indicates that the ²²⁶Ra accumulated in mussels is of natural rather than mine origin. The ²²⁸Th/²²⁸Ra ratio has been used to model radium uptake and a radium biological half-life in mussels of approximately 13 years has been determined. The long biological half-life and the low Ca concentrations in the water account for the high radium concentration factor of 30,000-60,000 measured in mussels from the Magela Creek catchment.     

观音岩水电站蓄水前后金沙江攀枝花江段鱼类群落结构及变化特征

基于2013~2017年金沙江干流攀枝花江段的鱼类资源监测数据，分析了观音岩水电站蓄水前后该江段的鱼类群落结构特征及变化情况。5年共监测到鱼类65种，长江上游特有鱼类共20种，5年均监测到的鱼类有12种，其中11种为喜流水性鱼类。各年度鱼类多样性指数呈现一定波动。ABC曲线及W统计表明2013和2014年鱼类群落结构未受到扰动，但在观音岩水电站蓄水运行后受到严重干扰。CA和NMDS分析显示鱼类群落可以分为两组，组I包括2013和2014年，组II包括2015、2016和2017年，one-way ANOSIM显示这2组在统计学上没有显著性差异。对聚类结果进行SIMPER分析结果显示，在观音岩水电站运行后，产漂流性卵的鱼类丰度明显降低，如圆口铜鱼、长鳍吻鮈等。因此，为了更好地保护攀枝花段的鱼类资源，应采取包括就地或迁地保护、生态调度和人工繁殖放流的针对性措施。     

Experimental studies on the biokinetics of 134Cs and 24Am in mussels (Mytilus galloprovincialis).

The biokinetics of 134Cs and 241Am in mussel species contaminated through water pathway has been studied under laboratory conditions. At equilibrium, the concentration factors for 134Cs and 241Am in small and large mussels were 2.80 and 2.57 and 200 and 150, respectively. The concentration factor of 134Cs in soft parts of the mussels was significantly high than whole body and shell tissue. However, the concentration factors of 241Am in soft parts and shell tissue samples were found to have similar rates. The depuration kinetics of the radionuclides were described by two-component exponential models. The biological half-lives at slow components between small and large mussels did not change significant, and were found to be 46.8-46.5 and 72.2-75.3 days for 134Cs and 241Am. The depuration kinetics of 134Cs and 241Am in soft parts described a single-component exponential model and the biological half-lives were found to be 29.4 and 41.1 days, respectively.     

Changes in the species composition and abundance of young-of-the-year fishes in the upper Volga stretch of the Kuibyshev Reservoir during the period of 1991 to 2009

The species composition and abundance of young-of-the-year fishes have been analyzed in catches made in the upper Volga stretch of the Kuibyshev Reservoir between 1991 and 2009. It has been found that the juvenile abundance of roach and blue bream has decreased, while that of perch, silver bream, and spined loach has increased, with young-of-the-year juveniles of common rudd, tubenose goby, round goby, and black-striped pipefish appearing in catches. The results of the study show that, against the background of juvenile population decline in many spring-spawning fish species, the abundance and species diversity of young of the year in summer-spawning species has increased during the observation period.     

崇西湿地冬季潮滩鱼类种类组成及多样性分析

根据2006年冬季在崇西湿地进行的光滩和草滩插网渔获调查,首次对冬季长江口潮滩鱼类种类组成、多样性及相关环境因子作了分析。结果表明：冬季崇西湿地潮滩鱼类主要由淡水小型鱼类组成,共23种,隶属7科,20属。光滩和草滩插网的最大优势种皆为油,两种网具的渔获种类组成具一定的相似性。在一个潮周期内,3种多样性指数D、H′ 和J值波动范围较大,小汛潮时多样性指数比其它潮汛低。白天和晚上潮水的鱼类种类数无显著变化（P>0.5）,但在个体数和生物量上白天较晚上潮水高（P<0.5）。水温、潮汐大小对潮滩鱼类丰度影响较大,而与盐度相关较小。此外,天气状况也是影响潮滩鱼类的一个重要因子。总之,潮滩鱼类的种类组成和多样性受到各种环境因子的综合影响。     

长江干流不同江段鱼卵及仔鱼漂流特征昼夜变化的初步分析

根据2007~2009年在长江干流的泸州、珞璜、宜都、武穴4个江段分别采集的数据,对这些江段鱼卵及仔鱼漂流特征的昼夜变化规律进行初步分析。结果显示,长江上游泸州江段和珞璜江段鱼卵及仔鱼昼夜漂流密度有一定差异,但不显著;长江中游宜都江段,鱼卵漂流密度在昼夜时间上有显著性差异（〖WTBX〗n=38,p〖WTBZ〗=000）,表现为6〖DK〗∶00的平均漂流密度13.02 ind./100 m3明显大于18〖DK〗∶00的密度3.28 ind./100 m3,仔鱼夜间漂流密度明显大于白天,于22〖DK〗∶00达到高峰值;武穴江段仔鱼漂流密度在昼夜时间上有显著性差异（〖WTBX〗n=62,p〖WTBZ〗=000）,8〖DK〗∶00的平均漂流密8621 ind./100 m3明显大于18〖DK〗∶00的密度4485 ind./100 m3。不同物种的鱼卵及仔鱼漂流密度亦呈现出一定的昼夜差异,宜都江段贝氏〖FK（W*5/5。1〗〖PS黎明政造字2.EPS;%84%84,BP〗〖FK)〗,草鱼和银鲴鱼卵主要分布在白天,鳙、鲢等种类分布在夜间;另外,鳙、银鮈、铜鱼和翘嘴鲌等多数种类的仔鱼仅在夜间有分布,而飘鱼属的种类主要分布在白天。通过采集到鱼卵发育期推算,四大家鱼、贝氏〖FK（W*5/5。1〗〖PS黎明政造字2.EPS;%84%84,BP〗〖FK)〗、银鲴和翘嘴鲌的繁殖时间主要集中在夜间,而花斑副沙鳅和鳊全天均有繁殖