Allelopathic response of <Emphasis Type="Italic">Vetiveria zizanioides</Emphasis> (L.) Nash on members of the family Enterobacteriaceae and <Emphasis Type="Italic">Pseudomonas</Emphasis> spp.

The biological removal of phosphates was carried as a part of treatment strategy. Vetiveria zizanioides (L.) Nash belonging to the family Poaceae was used for biological removal of biostimulants. Vetiver reportedly has mycorrhizal association; besides having potential for removal of PO₄ ⁻³ also showed allelopathic affect on the microorganisms present in the water. In fact after a period of 96 h old roots of this plant have been found to have killing effects on the E. coli, Enterobacter spp. Pseudomonas spp. belonging to the family Enterobacteriaceae. The paper is opening a new face of study.     

Production of vermifertilizer from guar gum industrial wastes by using composting earthworm <Emphasis Type="Italic">Perionyx sansibaricus</Emphasis> (Perrier)

Efforts have been made to convert the guar gum industrial waste into a value-added product, by employing a new earthworm species for vermicomposting e.g. Perionyx sansibaricus (Perrier) (Megascolecidae), under laboratory conditions. Industrial lignocellulosic waste was amended with other organic supplements (saw dust and cow dung); and three types of vermibeds were prepared: guar gum industrial waste + cow dung + saw dust in 40: 30: 30 ratio (T₁), guar gum industrial waste + cow dung + saw dust in 60: 20: 20 ratio (T_2,), and guar gum industrial waste + cow dung + saw dust in 75: 15: 10 ratio (T₃). As compared to initial concentrations, vermicomposts exhibited a decrease in organic C content (5.0–11.3%) and C:N ratio (11.1–24.4%) and an increase in total N (18.4–22.8%), available P (39.7–92.4%), and exchangeable K (9.4–19.7%) contents, after 150 days of vermicomposting. A vermicomposting coefficient (VC) was used to compare of vermicomposting with the experimental control (composting). P. sansibaricus exhibited maximum value of mean individual live weight (742.8 ± 21.1 mg), biomass gain (442.94 ± 21.8 mg), growth rate (2.95 ± 0.15 mg day⁻¹), cocoon numbers (96.0 ± 5.1) and reproduction rate (cocoons worm⁻¹ day⁻¹) (0.034 ± 0.001) in T₂ treatment. In T₃ maximum mortality (30.0 ± 4.01 %) in earthworm population was observed. Overall, T₂ vermibed appeared as an ideal substrate to manage guar gum industrial waste effectively. Vermicomposting can be proposed as a low-input basis technology to convert industrial waste into value-added biofertilizer.     

Effect of bioremediation on the growth of Okro (<Emphasis Type="Italic">Abelmoshus esculetus</Emphasis>) in the Niger Delta soils

This paper communicates the effect of bioremediation on the performance of Okro plant (Abelmoshus esculentus) in a typical Niger Delta soil that has received 5% crude oil pollution level. Biodegrading bacteria such as Pseudomonas fluorescen, Acinetobacteria iwofii, Bacillus subtilus, Arthrobacter globiformis that was isolated from previously polluted soils was introduced into the samples. The treatment combinations are as follows (A) = control without crude oil; B = soil + crude oil, (C) = soil + crude oil + microbes, (D) = soil + crude oil + microbes, (E) = soil + crude oil + microbes + fertilizer (F) soil + microbes and (G) = soil + fertilizer. The treatment (E) gave the highest number of leaves, % crop emergence, plant biomass, microbial population and degradation of petroleum hydrocarbon compared to any of the treatments that had received crude oil. This suggested that fertilizer application does not only stimulate microbial growth but it provides the plant with more available nutrients required for plant growth.     

Development of a sprayable slow-release formulation for the sex pheromone of the Mediterranean Corn Borer, <Emphasis Type="Italic">Sesamia nonagroides</Emphasis>

In the FAIR project “Pheromaize”, CT96-1302, the main objective is to provide European growers with a reliable, cost effective and environmentally friendly technology based on pest mating disruption. The project is mainly focused on Mediterranean Corn Borer (MCB), Sesamia nonagroides, the key pest of maize grown under Mediterranean conditions. TNO has developed a sprayable formulation consisting of a biodegradable matrix in which␣the pheromone is dissolved, together with a UV––stabilizer, an antioxidant, a surfactant and a sticker material. During outdoor exposure experiments release of pheromone was found to be high enough for more than 30 days. This formulation has been tested in large scale field experiments by helicopter spraying on 5 ha maize by field partners in Spain, Greece and France.     

Postfire Regeneration in <Emphasis Type="Italic">Pinus pinea</Emphasis> L. and <Emphasis Type="Italic">Pinus pinaster</Emphasis> Aiton in Andalucia (Spain)

The objective of this study was to examine postfire regeneration of tree, shrub, and dwarf shrub species, in relation to levels of damage in four planted pine forests (Pinus pinea, Pinus pinaster) in Andalusia. A prefire vegetation map was used for detailing species composition, vertical structure, and density and another for detailing the extent and intensity of fire damage. Between 3 and 7 years after the fires, an inventory was made of the vegetation in each area, using the step-point method. The information thus obtained was used to determine the amount of cover in the dwarf/shrub and tree layers, the frequency of species in each of the layers, floristic richness, and diversity (Shannon index). The botanical composition of the dwarf and shrub layer was analyzed using TWINSPAN. Variables were poorly correlated with level of fire damage, which suggests that the forests in this study followed the autosuccession model. Because of the artificial origin or seminatural condition, regeneration of the dominant tree species is poor, and it seems unlikely that forests will recover to their prefire state. Therefore action is recommended to restore these ecosystems.     

Application of magnetic field and iron in order to change medicinal products of <Emphasis Type="Italic">Ocimum basilicum</Emphasis>

In the present research, the effects of static magnetic field, with or without iron on the growth, the activity of certain enzymes (like polyphenol oxidase and phenyle alanine ammonialyase), the content of phenolic compounds and the essential oil composition of sweet basil (Ocimum basilicum) were studied. Treatment of plants with static magnetic field with or without iron led to decrease of the growth of plants as well as decrease in the activity of phenyle alanine ammonialyase and of phenolic compounds content. In addition in those plants exposed to magnetic field the amount of essential oils, notably of methyl chavicol, was increased. Exposure of plants to the magnetic field decreased the activity of polyphenol oxidase, whereas treatment of plants with iron when exposed to magnetic field increased polyphenol oxidase activity. In this group the content of essential oil was reduced.     

Phytoremediation of heavy metals by <Emphasis Type="Italic">Eichhornia crassipes</Emphasis>

Eichhornia crassipes was tested for its ability to bioconcentrate 8 toxic metals (Ag, Cd, Cr, Cu, Hg, Ni, Pb, and Zn) commonly found in wastewater from industries. Young plants of equal size were grown hydroponically and amended with 0, 0.1, 0.3, 0.5, 1.0, 3.0, and 5.0 mM of each heavy metal individually for 21 days. The test plant had the lowest and the highest tolerance indices for Hg and Zn, respectively. A significant (P ≤ .05) reduction in biomass production was observed in metal treated plants compared with the control. All strace elements accumulated to higher concentrations in roots than in shoots. Trace element concentrations in tissues and the bioconcentration factors (BCF) were proportional to the initial concentration of individual metal in the growth medium and the duration of exposure. From a phytoremediation perspective, E. crassipes is a promising plant species for remediation of natural water bodies and/or wastewater polluted with low levels of Zn, Cr, Cu, Cd, Pb, Ag and Ni.     

Growth and fecundity of earthworms: <Emphasis Type="Italic">Perionyx excavatus</Emphasis> and <Emphasis Type="Italic">Perionyx sansibaricus</Emphasis> in cattle waste solids

Epigeic earthworms (Oligochaeta) have been appeared as key organisms to convert organic waste resources into value-added products, i.e., vermicompost and worm biomass. The assessment of reproduction potential of composting earthworm may be beneficial for large-scale earthworm production. Although, the waste minimizing potential of Perionyx excavatus and Perionyx sansibaricus is well proved, but little information is available about their fecundity rate. In this study, the efforts have been made to explore the growth and reproduction biology of P. excavatus and P. sansibaricus, using cattle waste solid as culture substrate, under laboratory conditions. Earthworms were weighed weekly and number of cocoons produced per week assessed. Biomass productions, fecundity, maturation, natality all were significantly different between P. excavatus and P. sansibaricus. The highest mean individual biomass was 767.7 ± 18.4 mg and 612.6 ± 20.6 mg, respectively in P. sansibaricus and P. excavatus. However, the highest cocoon numbers occurred in P. excavatus (492.3 ± 13.6), significantly higher than P. sansibaricus (269.6 ± 17.1). Fecundity was slightly different in both species: 1.38 ± 0.77 cocoons adult worm⁻¹ week⁻¹ (P. excavatus) and 1.58 ± 0.74 cocoons adult worm⁻¹ week⁻¹ (P. sansibaricus). The hatchling success rate (%) was highest in P. excavatus. Overall natality (juveniles adult⁻¹ week⁻¹) was highest in P. sansibaricus (1.52) than P. excavatus (1.26), which suggests that P. sansibaricus may be a better candidate for rapid propagation of earthworms in cattle waste solid.     

Alteration in Rhizosphere Soil Properties of Afforested <Emphasis Type="Italic">Rhamnus lycioides</Emphasis> Seedlings in Short-Term Response to Mycorrhizal Inoculation with <Emphasis Type="Italic">Glomus intraradices</Emphasis> and Organic Amendment

The reestablisment of autochthonous plant species is an essential strategy for recovering degraded areas under semiarid conditions. A field experiment was carried out to assess the short-term effect of two reafforestation methods involving mycorrhizal inoculation and compost addition on soil quality parameters and Rhamnus lycioides seedling growth. The nutrient content (NPK) and enzymatic activities (dehydrogenase, urease, protease-BAA, acid phosphatase and β-glucosidase) increased and bulk density decreased in the rhizosphere soil with the organic amendment. Biomass C of rhizosphere soil increased by at least 240% with respect to the control soil after mycorrhizal inoculation and the combination of compost addition + mycorrhizal inoculation. Both mycorrhizal inoculation and composted organic residue addition increased R. lycioides seedling growth in the same proportion. In the short term, we conclude that the application of both reafforestation methods not only enhances the establishment of R. lycioides seedlings, but also improves soil quality.     

Investigating efficiency of <Emphasis Type="Italic">Lampito mauritii</Emphasis> (Kinberg) and <Emphasis Type="Italic">Perionyx ceylanensis</Emphasis> Michaelsen for vermicomposting of different types of organic substrates

The vermicomposting ability of Lampito mauritii (Kinberg) and Perionyx ceylanensis Michaelsen was evaluated by using three different types of organic substrates such as leaf litter of Polyalthia longifolia, Pennisetum typhoides cobs (pearl millet) and a weed, Rottboellia exaltata (whole plant except the roots) in combination with cowdung (1:1). Vermicomposting studies (120 days) conducted to optimize the number of worms required for efficient conversion based on the reduction of C/N ratio, percentage decomposition of organic substrates, total number and biomass of earthworms recovered from the vermibed substrates clearly showed that vermibeds with 4 kg of organic materials can hold about 60–80 L. mauritii and about 90–120 P. ceylanensis for efficient decomposition. The percentage decomposition of each organic substrate treated with different numbers of L. mauritii (20, 40, 60, 80 and 100 earthworms) and P. ceylanensis (30, 60, 90, 120 and 150) showed significant difference (P < 0.001) between numbers of worms introduced per vermibed but the difference between substrates was not significant within the treatments. Vermicomposting resulted in significant increase in electrical conductivity (28.54–49.82%), total nitrogen (43.96–90.83%), total phosphorus (27.42–68.10%) and total potassium (27.42–113.18%), whereas decrease in organic carbon (35.05–49.74%), C/N ratio (55.48–73.18%) and C/P ratio (50.46–66.90%) in different vermibeds introduced with L. mauritii and P. ceylanensis. Both the earthworm species can be used for vermicomposting different organic substrates; however, duration of vermicomposting with P. ceylanensis is not as much of L. mauritii. The use of L. mauritii for vermicomposting of other substrates has been well established by other workers also but standardization of P. ceylanensis, a locally available species, for vermicomposting of different organic substrates is a new finding and the species could be useful for vermiconversion of organic substrates under local conditions.     

Introduced Species and Management of a <Emphasis Type="Italic">Nothofagus/Austrocedrus</Emphasis> Forest

Isla Victoria (Nahuel Huapi National Park, Argentina), a large island dominated by native Nothofagus and Austrocedrus forest, has old plantations of many introduced tree species, some of which are famed invaders of native ecosystems elsewhere. There are also large populations of introduced deer and shrubs that may interact in a complex way with the introduced trees, as well as a recently arrived population of wild boar. Long-standing concern that the introduced trees will invade and transform native forest may be unwarranted, as there is little evidence of progressive invasion, even close to the plantations, despite over 50 years of opportunity. Introduced and native shrubs allow scattered introduced trees to achieve substantial size in abandoned pastures, but in almost all areas neither the trees nor the shrubs appear to be spreading beyond these sites. These shrub communities may be stable rather than successional, but the technology for restoring them to native forest is uncertain and probably currently impractical. Any attempt to remove the exotic tree seedlings and saplings from native forest would probably create the very conditions that would favor colonization by exotic plants rather than native trees, while simply clear-cutting the plantations would be unlikely to lead to regeneration of Nothofagus or Austrocedrus. The key to maintaining native forest is preventing catastrophic fire, as several introduced trees and shrubs would be favored over native dominant trees in recolonization. Deer undoubtedly interact with both native and introduced trees and shrubs, but their net effect on native forest is not yet clear, and specific management of deer beyond the current hunting by staff is unwarranted, at least if preventing tree invasion is the goal. The steep terrain and shallow soil make the recently arrived boar a grave threat to the native forest. Eradication is probably feasible and should be attempted quickly.     

Influence of different nitrogen rich supplements during cultivation of <Emphasis Type="Italic">Pleurotus </Emphasis><Emphasis Type="Italic">florida</Emphasis> on corn cob substrate

The cultivation of white rot edible fungus Pleurotus florida was performed in polybags. The corn cob was employed as basal substrate while eight different additives such as urea, ammonium sulphate, gram flour, soybean meal, ground nut cake and molasses were used with corn cob. Three different levels of variable combinations with corn cob were evaluated in response to different parameters of mushroom viz., mycelial growth, spawn running, primordial initiation, fruit body yield and its biological efficiency. Each additive at different combinations showed variable impact on the different stages of mushroom life cycle. The primordial initiation was observed for the first time during 20.2–35.1 days. The biological efficiencies in every supplemented set were increased over un-supplemented control set. Increasing the level of additives, the biological efficiency was negatively affected at higher levels. The cotton seed cake was found the best supplement producing 93.75% biological efficiency while soybean meal was the second best additive producing 93.00% yield. The highest growth rate, rapid mycelia run, early primordial initiation, highest yield and biological efficiency were recorded in the combination of corn cob and cotton seed cake at 2% (98 + 2) level.     

Norton’s <Emphasis Type="Italic">Sustainability</Emphasis>: Some Comments on Risk and Sustainability

Bryan Norton’s 2005 book Sustainability describes a pragmatic approach to environmental philosophy that stresses philosophy’s role as one of mediating between scientific and ordinary language. But on two topics, Norton’s approach is not pragmatic enough. In the case of his discussion of risk, he accedes to a scientific notion that fails to acknowledge the way that ordinary usage of the word risk involves pragmatic links to human action and moral responsibility. With respect to the word sustainability, his analysis fails to acknowledge important scientific work that characterizes the functional integrity of system cycling, opting instead for usage grounded either in economic accounting or in an even less substantive sense of a broad social movement for environmental improvement. On each of these topics, adherence to the pragmatic orientation of Norton’s philosophy results in a different analysis of the concepts in question.     

Physiological and Morphological Response Patterns of <Emphasis Type="Italic">Populus deltoides</Emphasis> to Alluvial Groundwater Pumping

We examined the physiological and morphological response patterns of plains cottonwood [Populus deltoides subsp. monilifera (Aiton) Eck.] to acute water stress imposed by groundwater pumping. Between 3 and 27 July 1996, four large pumps were used to withdraw alluvial groundwater from a cottonwood forest along the South Platte River, near Denver, Colorado, USA. The study was designed as a stand-level, split-plot experiment with factorial treatments including two soil types (a gravel soil and a loam topsoil over gravel), two water table drawdown depths (∼0.5 m and >1.0 m), and one water table control (no drawdown) per soil type. Measurements of water table depth, soil water potential (Ψ_s), predawn and midday shoot water potential (Ψ_pd and Ψ_md), and D/H (deuterium/hydrogen) ratios of different water sources were made in each of six 600-m² plots prior to, during, and immediately following pumping. Two additional plots were established and measured to examine the extent to which surface irrigation could be used to mitigate the effects of deep drawdown on P. deltoides for each soil type. Recovery of tree water status following pumping was evaluated by measuring stomatal conductance (g _s ) and xylem water potential (Ψ_xp) on approximately hourly time steps from before dawn to mid-afternoon on 11 August 1996 in watered and unwatered, deep-drawdown plots on gravel soils. P. deltoides responded to abrupt alluvial water table decline with decreased shoot water potential followed by leaf mortality. Ψ_pd and percent leaf loss were significantly related to the magnitude of water table declines. The onset and course of these responses were influenced by short-term variability in surface and ground water levels, acting in concert with physiological and morphological adjustments. Decreases in Ψ_pd corresponded with increases in Ψ_md, suggesting shoot water status improved in response to stomatal closure and crown dieback. Crown dieback caused by xylem cavitation likely occurred when Ψ_pd reached −0.4 to −0.8 MPa. The application of surface irrigation allowed trees to maintain favorable water status with little or no apparent cavitation, even in deep-drawdown plots. Two weeks after the partial canopy dieback and cessation of pumping, g _s and Ψ_xp measurements indicated that water stress persisted in unwatered P. deltoides in deep-drawdown plots.     

Impact of Busy Roads on Breeding Success in Pied Flycatchers <Emphasis Type="Italic">Ficedula hypoleuca</Emphasis>

The impact of dense traffic on the breeding success in pied flycatchers (Ficedula hypoleuca) was studied using roadside nest-boxes. Nest site selection and breeding success of flycatchers were observed in relation to the distance from the road. The number of occupied territories was no higher closer to the road than it was deeper inside the forest. The distance to the road had no effect on the laying date, clutch size, or brood size. However, nests closer to the road were more likely to fail at the chick stage. The number of broods that were closer to roads and were lost completely was significantly higher than those further away. As a consequence, the number of fledglings per breeding attempt decreased closer to roads. The nestlings typically died as older chicks when the parent birds were providing them with maximum quantities of food. A possible reason for the declining reproductive success was the traffic-related mortality of parent birds, as weather conditions were not especially adverse for raising healthy offspring.