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1.
The lichen Punctelia subrudecta (Nyl.) Krog was transplanted to 22 biomonitoring sites in a northwestern area of Córdoba city, Argentina and tested for chlorophyll a, chlorophyll b, phaeophytin a, soluble protein, hydroperoxy conjugated dienes (HPCD), malondialdehyde (MDA) concentration and sulphur accumulation. A pollution index (PI) was calculated for each biomonitoring site. The biomonitoring sites were set according to (1) traffic levels, (2) industrial density and (3) distance of the power station close to each site. The biomonitor's chemical response was associated with industries as well as power stations. Significant differences were observed in sulphur content, MDA concentration and PI values in lichen material transplanted to sites with different industrial densities. The higher values for these parameters were found at sites with high industrial levels. At the same time, significant differences were detected for sulphur content in samples at different distances from the power station, with higher accumulation in samples located far from the power plant. For MDA concentration, effect of different levels of industrial density was observed only at points close to the power station, probably because of higher levels of humidity near the power plant. For PI significantly higher values were observed in samples at points far from the power station and with high industrial density. This shows the additive effect of the principal emission sources that act on the response of P. subrudecta to air pollutants.  相似文献   

2.
The lichen Ramalina ecklonii (Spreng.) Mey. and Flot. was transplanted to 29 biomonitoring sites in the southeastern area of Córdoba, Argentina and tested for chlorophyll a, phaeophytin a, conjugated dienes concentration, malondialdehyde, soluble protein content and sulfur accumulation. The biomonitoring sites were determined according to (i) vehicular traffic levels, and (ii) industrial density, i.e. number and type of industries (small and medium) close to each of the sites. Each of the two groups were then broken down into three categories which provided a basis for performance analysis and quantification of the chemical parameters in the biomonitor. A pollution index (P.I.) was calculated based on the ratio of phaeophytin a to chlorophyll a and ratio of sulfur, malonaldehyde and conjugated dienes in transplanted specimens to sulfur, malonaldehyde and conjugated dienes in the freshly picked specimens. Significant differences were observed in sulfur content and P.I. in lichen samples that had been transplanted to sites with different vehicular traffic levels. At the same time, significant differences in the phaeophytin a concentration, phaeophytin a/chlorophyll a ratio and P.I. were observed at sites characterized by different levels of industrial density (all with low-to-medium traffic).  相似文献   

3.
The response of Usnea amblyoclada (Müll. Arg.) Zahlbr. to real concentrations of atmospheric pollutants measured by two automatic monitoring stations, was studied in Córdoba City, Argentina. The influence of different weather conditions on the biomonitor's response was also assessed. The concentration of chlorophyll a, chlorophyll b, hydroperoxy-conjugated dienes, and malondialdehyde were quantified in lichen thalli collected from a clean area and in transplanted thalli after 1 month of exposure in an urban area, from April to October, 1996. The dry weight/fresh weight, chlorophyll b/chlorophyll a and phaeophytin a/chlorophyll a ratios were also calculated. Data sets were evaluated by two-way analysis of variance and correlation analysis. It was observed that hydroperoxy-conjugated dienes, malondialdehyde, chlorophylls, and pigment degradation increase during winter-time, when higher levels of suspended particles, non-methane hydrocarbons, hydrogen sulfide and ozone were also measured. These findings would be connected mainly with meteorological conditions, as most pollutants did not exceed prescribed threshold levels.  相似文献   

4.
The suitability of a set of ecophysiological parameters, to be used as early warning indicator to detect signs of a worsening environment around geothermal power plants, was tested by comparison with the diversity of epiphytic lichens, a well-established indicator of geothermal air pollution. Samples of the lichen Evernia prunastri were transplanted around a geothermal power plant at Larderello (Tuscany, Italy) and at a control site, and integrity of cell membranes, concentration of chlorophyll a, b and carotenoids, chlorophyll integrity and variations in pH of thalli were measured. The results showed that cell membrane damage, expressed by changes in electrical conductivity, could be used to detect early (exposure periods as short as 1 month) deleterious effects of geothermal air pollution.  相似文献   

5.
This study examined the chemical response of Ligustrum lucidum Ait. and Ligustrum lucidum Ait. f. tricolor (Rehd.) Rehd. when exposed to different sources of atmospheric pollutants. Dry weight/fresh weight ratio, specific leaf area (SLA), sulfur content, chlorophyll concentration, carotenoids, soluble proteins, malondialdehyde (MDA) and hydroperoxy conjugated dienes (HPCD) were determined for leaf samples taken from different sites in the city of Córdoba, Argentina. These sites were categorized in terms of their traffic levels and industrial levels. Both L. lucidum as well as L. lucidum f. tricolor accumulated sulfur in their leaves in sites with high traffic levels, the former being the most sensitive to this type of pollution. Ligustrum lucidum exhibited significantly low concentrations of soluble proteins, in sites with high industrial pollution levels. Ligustrum lucidum f. tricolor did not exhibit a significant response to industrial pollution.  相似文献   

6.
The results of a survey aimed at investigating whether NO2 and NH3 emitted by road traffic can influence lichen diversity, lichen vitality and the accumulation of nitrogen in lichen thalli are reported. For this purpose, distance from a highway in a rural environment of central Italy was regarded as the main parameter to check this hypothesis. The results of the present survey indicated that road traffic is not a relevant source of NH3. On the other hand, NO2 concentrations, although rather low, were negatively correlated with distance from the highway according to a typical logarithmic function. No association between NO2 concentrations and the diversity of epiphytic lichens was found, probably because of the low NO2 values measured. Also bark properties were not influenced by distance from the highway. Accumulation of nitrogen, reduction in the content of chlorophyll a, chlorophyll b and total carotenoids were found in transplanted thalli of Evernia prunastri, but NO2 was not responsible for these changes, which were probably caused by applications of N-based fertilizers.  相似文献   

7.
The objective of the present study was to test the concentrations of some elements in the transplanted lichen Usnea amblyoclada transplanted in Córdoba, Argentina, and to investigate the relative air quality of the area as indicated by a Pollution Index. Analyses of Cu, Co. Pb, Fe, Ni, Mn, S and Zn in addition to analyses of physiological parameters were performed after the transplantation period. No significant differences were observed among the sampling stations for the physiological parameters, except the dry weight/fresh weight ratio. The concentration of most elements was similar to or lower than those found in non-polluted and even polluted areas. The significant correlation found between Cu, Pb and Zn with the content of hydroperoxy conjugated dienes suggests an important oxidative effect probably caused by these ions. The distribution patterns of the elements were quite similar, with maximum values around a cement plant and the metallurgical industries. The Pollution Index distribution pattern does not coincide with the elements distribution, due to the fact that the index values probably reflected the emissions of gaseous phytotoxic pollutants.  相似文献   

8.
In June of 1997, several thalli of the lichen species Alectoria sarmentosa were transplanted from a remote area (Bonavista) to an urban area (St John's) on the island of Newfoundland, Canada. The purpose was to assess the response of these epiphytic lichens to a change in the level of atmospheric sulphur pollution as measured by sulphur concentration and isotopic composition. The dominant source of atmospheric sulphur in the Bonavista area is sea spray, therefore, lichens growing there have relatively high sulphur isotopic compositions and low concentrations (approximately + 15 per/thousand, 250 ppm). Atmospheric sulphur in the St John's area is dominated by anthropogenic sources, primarily oil burning. Lichens in this area have lower isotopic compositions and higher concentrations (approximately + 6 per thousand, 500 ppm). The transplanted lichens were monitored monthly for a period of 1 year. In all experiments the sulphur isotopic composition decreased and the sulphur concentration increased linearly. It is estimated that, within 18 months, transplanted A. sarmentosa would be indistinguishable from the same species naturally growing in the transplant site, both in terms of sulphur concentration and isotopic composition.  相似文献   

9.
The present study was focused on the effect of increasing urbanization including industrial and traffic activity on the accumulation of heavy metals and possible damage of selected physiological parameters (composition of assimilation pigments, membrane lipid peroxidation, and membrane integrity) of an epiphytic foliose lichen, Flavoparmelia caperata (L.) Hale. The lichen samples were collected from three different localities in and around Kolkata, India, two sites being from the urban area and one from the relatively non-polluted sub-urban area. The results showed that thalli from the urban sites have significantly higher concentrations of Fe, Cr, Cu, Zn, and Pb compared to those collected from the sub-urban site. Physiological parameters of damage also exhibited stress symptoms in thalli from the urban sites—decreased chlorophyll a indicating less photosynthetic efficiency, and increase in lipid peroxidation and electrolyte conductivity indicating cell membrane injuries. Correlation studies among metals pinpointed vehicular traffic as the main source of pollution in this area.  相似文献   

10.
The paper reports a case of evident and widespread leaf damage on trees in southern Tuscany (Central Italy) attributed to the input of pollutants produced in a geothermal area. The main potentially phytotoxic substances are boron and hydrogen sulphide. Trees affected are conifers as well as both evergreen and deciduous broadleaves. In the present study the possible impact of geothermal pollutants on Quercus pubescens leaves has been considered. Leaf samples coming from three sampling locations (S1 inside the geothermal area; S2 on the margins; S3 outside) and three consecutive dates (June, July and August) were analyzed for the following parameters: sulphur and boron concentration; leaf area; leaf mass per area; chlorophyll fluorescence (Fv/Fm); chlorophyll a, chlorophyll b and carotenoid concentrations. Anatomical and ultrastructural observations were also performed. In all sampling location sulphur and boron concentrations are greater than the background values recorded in southern Tuscany in a previous survey. The sulphur concentration in leaves was higher in S1 than S2 and S3, but did not increase throughout the survey period. Boron reached the greatest concentrations in S2 and showed a continuous increase over the study period. Leaves subjected to a higher load of pollutants were smaller in size (in terms of leaf area), but were more sclerophyllous. Damaged chloroplasts and reduced Fv/Fm values were observed at S1 and S2, but chlorophyll concentration values were higher at S1. Such an apparent anomaly can possibly be explained by the onset of compensation and recovery mechanisms. Foliar injuries appeared to be related to boron concentration.  相似文献   

11.
Goals, Scope and Background Among other substances, sulphur dioxide (SO2), nitric oxide (NO) and nitrogen dioxide (NO2) are parameters which are routinely measured to describe basic air quality. Organic extracts of airborne particulate matter contain mutagenic chemical compounds of different origins. The aim of the study was to find correlations between routine monitoring data and mutagenic activity of organic extracts of simultaneously drawn samples.Methods Specimens were collected over a period of two years at 8 sampling sites in south-west Germany. Simultaneously, concentrations of NO, NO2, and SO2 were measured on-line within the framework of the official air monitoring network of Baden-Württemberg, Germany. Dust samples were collected for biotesting using high volume air samplers equipped with glass fibre filters. After sampling was completed, filters were extracted and samples were prepared for biological testing. Mutagenic activity was tested by means of the plate incorporation assay (Ames test) using S. typhimurium TA98 and TA100 tester strains. During the first year of the study, all tests have been performed with and without metabolic activation. Additionally, a series of tests has been performed in parallel with TA98 and TA98NR.Results and Discussion Comparison of Ames test data obtained with and without metabolic activation indicates no statistically significant difference between both methods. Therefore, during the second year of the study, all tests have been performed without metabolic activation. Average yearly activities at the sampling sites were between 1 und 27 Revertants per m3 (Rev/m3). High activities were preferably found at congested sites (Karlsruhe, up to 95 Rev/m3). However, peak values of over 100 Rev/m3 were found in other places where pollution by traffic is significantly lower. The reason for these high level values is not evident. Tests performed using TA98NR tester strain indicate a significant share (average 31%) of compounds requiring activation by nitroreductase for mutagenic activity. Average mutagenic activity can be correlated to routine monitoring parameters. Comparison of averaged data for particular sampling sites indicates significant correlation between nitric oxide and mutagenic activity in TA98 (r2=0.90), while correlation between nitrogen dioxide (0.84) or sulphur dioxide (0.52) and mutagenic activity is weaker. For TA100, correlations are generally weaker than for TA98. Comparison of data for mutagenic activity and routine monitoring data of distant sites being sampled simultaneously shows parallel behaviour.Conclusions Results from this study show that mutagenic activity can be compared to seasonal and local variations of gaseous indicator air pollutants. Tester strain TA98 generally shows the best correlations. Although pollution by particle-bound mutagenic substances is significantly higher during the cold season than during summer on average, mutagenic activity of airborne dust is not a continuous effect. During winter, peak levels as well as low pollution periods can occur. Even during winter time mutagenic activity can reach very low levels typical for summertime. Comparison of results for distant sampling sites where samples have been collected simultaneously indicate that “classical” indicators of air pollution and bacterial mutagenicity of organic extracts from airborne particulate matter are influenced by connected effects. Seasonal trend of mutagenic activity, in particular, is similar to the concentrations of nitrogen oxide. NO is a strong indicator for vehicle exhaust gases. It is concluded that the average mutagenic activity at particular sites can be estimated using NO concentrations as an indicator.  相似文献   

12.
Epiphytic lichens were sampled on mango trees (Mangifera spp.) in 32 sites in urban centres and surrounding rural areas in seven provinces of upper northern Thailand. Species were recorded on each tree and frequency estimated in a grid of ten 100-cm2 units. Analysis of macrolichen data showed that lichen diversity was inversely correlated with human population, and that lichen diversity was lowest in the cities with the highest population. The distribution and frequencies of species belonging to families Physciaceae and Parmeliaceae contributed to the groups identified by cluster analysis that corresponded to gradients in rainfall and population density. Comparison with modelled pollution data and local pollution records showed greater correspondence of lichen data with the effects of PM10 than with anthropogenic sulphur.  相似文献   

13.
An extensive investigation was carried out for the characterisation of the air particulate composition in Florence. The aim was to determine the aerosol elemental concentrations, as well as to identify pollution sources. For our investigation, the external Particle-Induced X-Ray Emission-Particle-Induced gamma-Ray Emission beam facility of the Istituto Nazionale di Fisica Nucleare, Van de Graaff accelerator at the Physics Department of the Florence University was used. We report the results of the analysis of a long temporal series (approximately 1 yr) of PM10 particulate samples, collected on Millipore filters on a daily basis in three different sites (characterised by different urban settings). Daily concentrations of more than 20 elements were detected. The long sampling period (approximately 1 yr) allowed a comparison with the air quality recommended values and the identification of seasonal variations. Four main sources (traffic, oil-combustion, soil-dust, and wind transported sea-salt) were extracted with the help of Principal Component Analysis (PCA). An absolute PCA showed traffic to be the major source both in the high traffic site and in the urban background site.  相似文献   

14.
The lichen Ramalina celastri was employed as an in situ biomonitor for the study of air quality and heavy metal distribution in the central region of the Argentine Republic. The thallus content of copper, iron, manganese, cobalt, zinc, nickel and lead was determined by atomic absorption spectrophotometry. Pigments, hydroperoxy conjugated dienes, malondialdehyde and sulfur were also measured. Some of these parameters were used to calculate a pollution index. Geographical distribution patterns were obtained for the different metals by mapping. Thus, zinc shows high levels in industrial and agricultural zones. The highest nickel values were found in urban-industrial zones located at the centre and south of the study area. The same was observed for sulfur. The pollution index distribution map showed that major physiological damage in the biomonitor was found in central and southeastern areas that coincide with Cordoba City and agricultural zones. So, the effect would seem to be related to urban activities and the use of pesticides.  相似文献   

15.
Samples of the vascular plant Tradescantia pallida and the lichen Usnea amblyoclada were exposed from October 2004 to April 2005 in three sites with different local sources of air pollution in Córdoba city, Argentina. Simultaneous determinations of the ambient levels of total suspended particles were made for each site. Young inflorescenses of T. pallida were collected in November, February and April and the frequency of micronuclei was determined on early tetrads of pollen mother cells. Physiological parameters and the elemental composition of lichen thalli were measured from samples exposed and replaced every month. Significant differences among sampling sites were observed in the frequency of micronuclei measured in T. pallida as well as in many physiological parameters and elements accumulated in lichen thalli. The mass of particulate material as well as the concentration of Ca, Mn, Cu, Zn and Sr was significantly different in different sampling sites, too. These results suggest that in situ biomonitoring using both higher plants and lichens may be of use to characterize air pollution in areas devoid of instrumental monitoring techniques or where it is necessary to explore the distribution of air contaminants at a microscale.  相似文献   

16.
Oryza sativa L. cv. GR3 was grown near to a fertilizer plant and the growth of its seed progeny was evaluated at sites near to, and distant from, the fertilizer plant. The grain yield was reduced in plants grown at the polluted site, with reduced panicle length and increased sterility index. This decrease in grain yield was higher in the progeny plants than in the normal polluted plants. The polluted progeny grown at a control site showed a normal yield, but an increased sterility index. Accumulation of total sulphur and fluoride was higher in the leaves of polluted rice plants. It was evident that tolerance to air pollution was reduced in the progeny plants due to an additional dosage of sulphur and fluoride pollutants and to unfavourable climatic factors. Further, pollution effects were not carried over in progeny plants when grown in an unpolluted environment.  相似文献   

17.
Distribution of heavy metal contents of urban soils in parks of Seville   总被引:28,自引:0,他引:28  
Several metals in urban soils of Seville were extracted by two methods, which respectively give estimates of the available and ‘quasi total’ contents. Although the soils did not show strong heterogeneity in their general properties, high dispersion of the contents in those metals with greater relative availability, Cu, Pb and Zn, as compared to others suggested that pollution with these three metals could occur in some sampling sites. It was shown that the contents in these metals tend to increase as the historic quarters of the city are approached. Using reference values given by the Québec Ministry of Environment it was shown that those green areas closer to the historic centre present contents in Pb, Zn and particularly Cu that often exceed the acceptable limits for residential, recreational and institutional sites. From background contents for Seville soils estimated from a park located on the outskirts, a pollution load index (PLI) for each sampling site was calculated for the set of these three metals. It was shown that the PLI tends to increase as traffic density increases and as distance from main traffic decreases, but poor regressions were obtained, suggesting that other variables different from traffic should be considered.  相似文献   

18.
Vertical snow sampling and moss bag transplants were used to estimate the local inorganic and organic pollutant load deposited from traffic along a major highway in Finland. The pH and concentrations of Cl(-), NO(3)(-), SO(4)(2-), Ca(2+), Na(+) and polyaromatic hydrocarbons (PAHs) were determined from snow samples collected in winter at different sites along the highway. In summer, moss bags containing 20 g of fresh red-stemmed feather moss (Pleurozium schreberi) were transplanted at the same sites. The moss bag transplants remained exposed to roadside traffic for a period of one month following which the samples were collected and the PAH profiles and concentrations were analysed. The deposition of inorganic and organic pollutants from road traffic was observed up to 60 m from the road. The prevailing winds had a significant effect on the dispersion of pollutants. Snow appears to be a good collector of inorganic pollutants from the atmosphere and can be used to monitor local airborne pollution from road traffic. Snow packs can also be used as passive collectors of organic pollutant loads from road traffic on a local scale. To monitor organic PAH deposition from the road traffic, moss bags appeared to be better indicators compared to snow sampling. The efficiency of moss bags in accumulating PAH compounds indicate that vegetation may be an important sink for traffic pollution.  相似文献   

19.
Abstract

An extensive investigation was carried out for the characterisation of the air particulate composition in Florence. The aim was to determine the aerosol elemental concentrations, as well as to identify pollution sources. For our investigation, the external Particle-Induced X-Ray Emission–Particle-Induced γ-Ray Emission beam facility of the Istituto Nazionale di Fisica Nucleare, Van de Graaff accelerator at the Physics Department of the Florence University was used. We report the results of the analysis of a long temporal series (approximately 1 yr) of PM10 particulate samples, collected on Millipore filters on a daily basis in three different sites (characterised by different urban settings). Daily concentrations of more than 20 elements were detected. The long sampling period (approximately 1 yr) allowed a comparison with the air quality recommended values and the identification of seasonal variations. Four main sources (traffic, oil-combustion, soil-dust, and wind transported sea-salt) were extracted with the help of Principal Component Analysis (PCA). An absolute PCA showed traffic to be the major source both in the high traffic site and in the urban background site.  相似文献   

20.
The sulphur isotope composition of the epiphytic lichens, Hypogymnia physodes (L.) Nyl and Pseudevernia furfuracea (L.) Zopf, of the terricolous lichens, Cladina sp. and Peltigera aphthosa (L.) Willd., and of pine bark, at 31 sites in Finland, appeared to be dependent on lichen and tree species. The negative correlation of sulphur isotope composition with sulphur content was highly significant (P<0.001) in H. physodes and significant (P<0.01) in Pseudevernia furfuracea and Peltigera aphthosa. No correlation between the sulphur isotope composition and sulphur content was found for Cladina sp. and pine bark. The sulphur isotope compositions displayed statistically significant regional variation from south to north. Microenvironmental factors were considered, including phorophyte species and the chemical form of sulphur.  相似文献   

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