Viruses in surface and drinking waters

Over 114 different human enteric viruses are known. They are all excreted into sewage, and opportunities exist for them to find their way into water distribution systems. The characteristics of the illnesses caused by enteric viruses make their transmission by water difficult to recognize. Methods are not available for isolating all of the enteric viruses from water, so that finding any type of human virus in drinking water is an indication that other types may also be present. With improvements in technology, our laboratory has recently been able to detect group B coxsackieviruses, rotaviruses, and hepatitis A virus in chlorinated drinking water. At present, there are no uniform methods for concentrating, isolating, and identifying viruses in water. Emphasis should be placed on seeking uniformity in methods so that standards can be set for viruses in water.     

Viruses on shellfish-growing waters

The conversion of the bivalve shellfish to a virus carriage status is the most important consequence of the virus pollution of shellfish-growing waters. Virus carriage has public health implications of significance for consumers of raw or inadequately cooked shellfish, as well as general biological implications of significance for shellfish themselves. The nonculturable human enteric viruses are responsible for most if not all of the illness transmitted by virus-carrying shellfish. Use of feces-associated natural virus in numbers comparable to those found in grossly polluted waters has been instrumental in developing new perspectives of virus carriage and its biological significance. The feasibility of developing a virus standard for virus surveillance of shellfish and its use for assessment of health risks which aris as a consequence of shellfish virus carriage is discussed.     

Decreased influenza virus pathogenesis by infection with germicidal UV-irradiated airborne virus

Respiratory infections are acquired by the inhalation of airborne microorganisms. To evaluate the effect of germicidal UV-irradiation on airborne contagion, infectious clouds of influenza A/PR8/34 virus were generated and exposed to known intensities of UV. Thereafter, the airborne virus was used to infect mice wherein the pathogenesis of the viral pneumonia was evaluated. Increasing doses of UV inactivated infectious airborne virus in a dose-dependent manner and reduced the mortality rates as compared to an infectious cloud of untreated virus. When a sublethal cloud of infectious virus was used, UV-irradiation modified the viral infection, as quantitated by pulmonary virus titers, from a severe pneumonitis to a milder form of the disease. These data demonstrate the efficacy of germicidal UV-irradiation in disinfection of air by reducing the concentration of viable organisms which, in turn, reduces the severity of respiratory infection when transmitted to the susceptible host.     

Quality of currently available methodology for monitoring viruses in the environment

A variety of methods have been proposed, developed, and evaluated for detecting viruses, especially human enteric viruses, in water, wastewater, and other environmental samples, and continued developments and improvements have led to simpler and more reliable methodology. However, the use of currently available methods is still limited primarily to special circumstances, such as investigation of waterborne disease outbreaks, research studies on virus reductions by water and wastewater treatment processes and systems, especially reuse systems, and research monitoring and surveying of natural and treated waters. Widespread and routine virus monitoring is still not possible due to technical limitations and deficiencies of present detection methods and their relatively high cost. Although continued development of new methods and further improvement of existing methods is desirable, it is perhaps more important that current methods be systematically evaluated in carefully designed collaborative (round-robin) studies and quality assurance tests. Until virus detection methods are further improved and systematically evaluated, the establishment of virus standards for such materials as water and edible shellfish requiring routine monitoring appears to be unjustified.     

Human pathogens and their indicators in biosolids: a literature review

A growing beneficial reuse of biosolids in agriculture has led to concerns about potential contamination of water resources and the food chain. In order to comprehend the potential risks of transmission of diseases to the human population, an advanced quantitative risk assessment is essential. This requires good quantitative data which is currently limited due to the methodological limitations. Consequently, further development and standardization of methodologies for the detection, enumeration and viability assessment of pathogens in biosolids is required. There is a paucity of information on the numbers and survival of enteric virus and protozoan pathogens of concern in biosolids. There is a growing urgency for the identification of more reliable alternative indicators, both index and model microorganisms, which could be used for potential public health risk assessment. In this review, we have summarized reported literature on the numbers and fate of enteric pathogens and indicators in biosolids. The advantages and limitations of the use of conventional and alternative index and model microorganisms for the prediction of pathogen presence in biosolids are also discussed.     

A review of virus and protein sorption by ion exchange resins

This paper reviews the use of ion exchange resins for adsorbing proteins and viruses and for purifying viral suspensions. The analysis of resin performance under different conditions provides some information on the fundamental nature of virus/protein-resin interaction.A variety of systems are considered and the influence of factors such as resin type, resin pretreatment, chemical characteristics of the solution and virus type on adsorption are discussed and compared. Analogies are made between virus-host and virus-resin interactions, both of which are specific to the virus-host or virus-resin pair. Consideration is given to the use of proteins as models for virus adsorption. The use of viral parameters such as iso-electric point and the amino acid content of the protein coat for predicting virus adsorption behavior is also discussed. Anion exchange resins were found to be more effective virus sorbents than cation exchange resins. Cation exchange resins were ineffective in low ionic strength media, while anion exchange resins were most effective in low ionic strength media.     

Viruses in groundwaters

There is no longer any question as to the presence of human enteroviruses in secondary sewage effluents. Sufficient data are available to indicate that viruses can and do percolate through the soil and move with the groundwater. Also, there is no question that a significant number of human illnesses of viral etiology have been associated with the consumption of untreated or chlorinated groundwater obtained from sewage contaminated wells. Although classical epidemiological investigative evidence linking groundwater with enterovirus disease transmission is not currently available, the alternative data presented in this report indicate that virus in groundwater is of sufficient significance to public health to warrant increased viral surveillance of groundwater. Concurrently, efforts should be directed toward increasing enterovirus disease surveillance and reporting to facilitate the earliest possible recognition of an outbreak and the initiation of an epidemiological investigation. Only under these conditions will the magnitude of the significance of viruses in groundwater be elicited.     

Protein futures for Western Europe: potential land use and climate impacts in 2050

Multiple production and demand side measures are needed to improve food system sustainability. This study quantified the theoretical minimum agricultural land requirements to supply Western Europe with food in 2050 from its own land base, together with GHG emissions arising. Assuming that crop yield gaps in agriculture are closed, livestock production efficiencies increased and waste at all stages reduced, a range of food consumption scenarios were modelled each based on different ‘protein futures’. The scenarios were as follows: intensive and efficient livestock production using today’s species mix; intensive efficient poultry–dairy production; intensive efficient aquaculture–dairy; artificial meat and dairy; livestock on ‘ecological leftovers’ (livestock reared only on land unsuited to cropping, agricultural residues and food waste, with consumption capped at that level of availability); and a ‘plant-based eating’ scenario. For each scenario, ‘projected diet’ and ‘healthy diet’ variants were modelled. Finally, we quantified the theoretical maximum carbon sequestration potential from afforestation of spared agricultural land. Results indicate that land use could be cut by 14–86 % and GHG emissions reduced by up to approximately 90 %. The yearly carbon storage potential arising from spared agricultural land ranged from 90 to 700 Mt CO₂ in 2050. The artificial meat and plant-based scenarios achieved the greatest land use and GHG reductions and the greatest carbon sequestration potential. The ‘ecological leftover’ scenario required the least cropland as compared with the other meat-containing scenarios, but all available pasture was used, and GHG emissions were higher if meat consumption was not capped at healthy levels.     

State-of-the-art sustainability analysis methodologies for efficient decision support in green production operations

Over the last three decades, new concepts, strategies, frameworks and systems have been developed to tackle the sustainable development issue. This paper reviews the challenges, perspectives and recent advances in support of sustainable production operations decision-making. The aim of this review is to provide a holistic understanding of advanced scientific analysis methodologies for the evaluation of sustainability, to provide efficient decision support. Over 100 publications have been analysed, and a characterisation of state-of-the-art sustainability analysis methodologies has been produced, which includes life cycle assessment and multi-criteria decision analysis (MCDA), along with their applications to three key areas of production operations: sustainable design, sustainable manufacture and sustainable supply chain management. Distribution of existing work is discussed and future research directions are elicited from the literature. The paper finds three trends in supporting sustainable production operations decisions: (a) sustainability analysis has moved to whole life cycle assessment from single-stage assessment, (b) sustainability analysis has shifted away from single criterion to MCDA and (c) sustainability analysis has evolved from stand-alone approaches to integrated systematic methodologies. The paper concludes that integrated sustainability analysis can provide more efficient and effective support to complex decision-making in sustainable production operations.     

Viruses in wastewaters

Viruses of animals, plants, and bacteria abound in sewage and receiving waters. Their ecological impact has, for the most part, gone unheeded except as it relates to viruses from human sources. Viruses present at levels infective to man have been recovered from waters used for recreational or drinking purposes. Their presence in a water environment virtually always denotes prior contamination by domestic wastes. Neither conventional sewage treatment processes nor the discharge to land or water of sludges produced by these processes achieve full viral control. Many environmental virologists advocate the setting of permissible virus limits for those recreational and potable waters dominated by wastewater effluents. The initiation of regulatory pressure to restrict virus discharges into these water environments has been instituted in Montgomery County, Maryland, and in the states of California and Arizona.     

Assessing the risk of wind-borne spread of foot-and-mouth disease in Australia

The effect the environment has on the spread of animal disease depends on the particular disease and can vary from being minor to being almost the sole determinant. An assessment of the risk of a particular disease outbreak and its consequences must combine the epidemiology of the disease with the variability of the environment. Foot-and-mouth disease is a highly contagious animal disease that spreads readily by close contact with infected animals and infected product. For such spread, the environment has little consequence. However, occasionally, wind-borne viral particles can spread the disease considerable distances. This paper concentrates on the methods used in a study undertaken to assess the potential for the wind-borne spread of foot-and-mouth disease under Australian conditions. The conditions suitable for the virus to persist as an aerosol, the amount of virus produced during an outbreak, and the dose-response to wind-borne virus were determined by considering the epidemiology of the disease. Environmental components considered included an analysis of weather records and livestock distribution data to identify areas at risk, and the use of a Gaussian plume model to estimate the extent of spread.     

长江中下游地区畜禽承载力评估与预警分析

为评估长江中下游地区畜禽养殖的环境风险和承载潜力,以《畜禽养殖业产污系数与排污系数手册》和《畜禽粪污土地承载力测算技术指南》中的相关参数为基础,采用2016年的统计数据,测算了长江中下游地区的畜禽粪便总量、耕地畜禽粪污氮磷负荷和耕地畜禽养殖环境容量。结果表明：（1）2015年长江中下游地区畜禽粪便总量为44 784.09万t,单位耕地畜禽粪污氮、磷负荷分别为71.18、12.71 kg/hm2,畜禽养殖环境容量76 561.60万头猪当量（N）、106 208.50万头猪当量（P）,环境风险指数为0.68（N）、0.84（P）,养殖风险中等,具有一定发展潜力,增量规模分别为实际养殖总量的47%（N）、19%（P）;（2）分省域来看,畜禽粪便资源丰富程度由高到低依次为湖南、湖北、安徽、江西、江苏、浙江和上海;耕地氮负荷由大到小依次为湖南、湖北、江西、安徽、江苏、上海和浙江;耕地磷负荷由大到小依次为湖南、湖北、江西、江苏、安徽、上海和浙江;上海、江苏、浙江、安徽、湖北和湖南畜禽养殖环境风险中等,具有发展潜力,可适当增加养殖数量;江西畜禽养殖环境风险较严重,不具有发展潜力,需要进行总量控制;（3）分市域来看,约59%的市畜禽养殖环境风险中等,约28%的市畜禽养殖环境风险较严重,约10%的市畜禽养殖环境风险较小,仅3%的市畜禽养殖环境风险严重。需按照重点调控区、约束发展区、适度发展区、潜力增长区和重点发展区进行优化,并根据区域种植业结构进行动态调整。     

四川省畜禽粪便排放时空分布及污染防控

掌握畜禽粪便的时空分布,有利于高效防控其污染环境的可能。采用排污系数法,估算出2000～2015年间四川省畜禽粪便、COD及氮磷产生量,在此基础上,分析了2015年四川省畜禽粪便的排放时空分布特征与畜禽粪便、氮、磷污染情况。结果表明：（1）2000～2015年,四川省畜禽粪便平均产生量约2.10亿t猪粪当量,其中猪、牛、家禽是当地环境威胁的主要畜禽种类;（2）2015年四川省产沼气潜力可观,但大部分地区受到畜禽粪便污染,其中德阳市、雅安市两地预警级别达Ⅵ级,对环境影响严重;（3）2015年四川省氮、磷耕地负荷的平均值分别为78.45 kg/hm2和13.57 kg/hm2,均低于欧盟的限量标准（氮170 kg/hm2、磷35 kg/hm2）,但大部分地区面临氮、磷污染风险。其中成都市、德阳市、雅安市氮、磷污染风险均较高,自贡市、泸州市等氮、磷污染风险整体不高,但畜禽养殖量近50%环境容量,这些地区应做好畜禽养殖总量控制及污染物消减措施。 关键词： 畜禽养殖;粪便污染;时空分布;污染防控;四川     

基于效果-效率-适应性的养殖废弃物资源化利用管理模式评价框架构建及初步应用

由于产业发展正处于转型阶段,我国畜禽养殖污染治理中的管理问题比技术问题更加突出,选择适宜的组织管理模式尤其重要。融合了效果分析、效率评价和适应性分析,尝试构建一个养殖废弃物资源化利用管理模式综合评价框架,并选择太湖流域4个不同类型的典型案例进行方法例证。研究表明,传统的养殖企业主导型管理模式应用于小型、分散养殖废弃物资源化利用时面临着经济效率低、适应性差难题;农村废弃物处理中心、种植企业和有机肥企业的参与可以改善小型、分散养殖废弃物资源化利用的经济效率;综合考虑效果、效率与适应性,种植企业、有机肥企业主导型模式的适宜推广范围更广。应根据不同地区种植-养殖产业关系、污染治理需求、经济发展水平与政府投入能力等,灵活组合应用不同管理模式。     

Life cycle assessment environmental data for structural steel construction in Greece

The construction industry is a critical sector in relation to sustainable development as its optimisation in terms of the consumption of resources can provide significant environmental benefits. To achieve this, a number of tools and methodologies have been developed to assist decision making. Life cycle assessment is one of the most acknowledged and widely used methodologies to assess the sustainability of construction works. Its efficient use, however, requires environmental data that in some cases are not yet available. Especially for sectors such as steel construction in which the potential for sustainable development is increased due to the recycling and reuse potential of the material, the lack of such data slows sustainability-related progress down. The current research addresses this issue by collecting primary environmental data for cold-formed structural steel and compiling the required data-sets. The data-sets are used to assess the environmental impact of an existing steel building and identify how and to what extent the environment is burdened by its construction. The findings of the research include the aforementioned environmental data which can be used in environmental analyses related to construction projects, while additional conclusions concerning the environmental impact of steel buildings in terms of quantity and type were also drawn.     

A guide towards climate change adaptation in the livestock sector: adaptation options and the role of robust decision-making tools for their economic appraisal

Economic appraisal and technical effectiveness of adaptation options are key criteria for judging climate change adaptation investment decisions in all sectors. Yet relatively little methodological guidance exists for determining the most appropriate appraisal techniques for different adaptation options. This paper provides adaptation options and scopes relevant appraisal methods in agriculture focussing on livestock production specifically. We find that for many adaptation options for livestock agriculture, standard (expected) cost-benefit analysis is an appropriate tool. For adaptation options requiring long lead times or those with long lifetimes, techniques incorporating uncertainty (‘robust’ methods) are more suitable, including real options analysis, portfolio analysis and robust decision-making. From a comprehensive list of adaptation options in the livestock sector, we identify the most appropriate appraisal technique for each option and describe how the robust appraisal tools could be applied to heat stress, flood risk and water management.

     

Risk management of viral infectious diseases in wastewater reclamation and reuse: Review

Inappropriate usage of reclaimed wastewater has caused outbreaks of viral infectious diseases worldwide. International and domestic guidelines for wastewater reuse stipulate that virus infection risks are to be regulated by the multiple-barrier system, in which a wastewater treatment process composed of sequential treatment units is designed based on the pre-determined virus removal efficiency of each unit. The objectives of this review were to calculate representative values of virus removal efficiency in wastewater treatment units based on published datasets, and to identify research topics that should be further addressed for improving implementation of the multiple-barrier system. The removal efficiencies of human noroviruses, rotaviruses and enteroviruses in membrane bioreactor (MBR) and conventional activated sludge (CAS) processes were obtained by a systematic review protocol and a meta-analysis approach. The log₁₀ reduction (LR) of norovirus GII and enterovirus in MBR were 3.35 (95% confidence interval: 2.39, 4.30) and 2.71 (1.52, 3.89), respectively. The LR values of rotavirus, norovirus GI and GII in CAS processes were 0.87 (0.20, 1.53), 1.48 (0.96, 2.00) and 1.35 (0.52, 2.18), respectively. The systematic review process eliminated a substantial number of articles about virus removal in wastewater treatment because of the lack of information required for the meta-analysis. It is recommended that future publications should explicitly describe their treatment of left-censored datasets. Indicators, surrogates and methodologies appropriate for validating virus removal performance during daily operation of wastewater reclamation systems also need to be identified.     

Chemical tracers as indicator of human fecal coliforms at storm water outfalls

Indicators to distinguish between fecal coliforms of human and animal origin were investigated in water from storm sewer outfalls to a coastal lake during wet and dry weather. The ratio of fecal coliform relative to fecal streptococci count was used as the microbiological indicator. Concentrations of human-activities originated caffeine, anionic surfactant, fluoride, and fluorescence whitening agent (FWA) were used as chemical indicators. The ratio of fecal coliform to fecal streptococci ranged from 0.2 to 3.0, during wet weather making it difficult to interpret the origin of fecal pollution. However, concentrations of caffeine, anionic surfactant, fluoride, and FWA in storm water outflow during wet weather were much higher than those in the lake water during dry weather, indicating the presence of human waste at storm water outfall. Strong correlation between fecal coliform counts and chemical parameter values further indicated the human contribution to the fecal coliform count. In addition, a strong correlation among the chemical parameters suggested that only one of them is needed as chemical tracer to detect the presence of human input.     

Let’s play transformations! Performative methods for sustainability

Coping with global environmental change demands new forms of civic engagement and interaction able to transform passive audiences attending to the drama of unsustainability into committed actors for sustainability. This entails linking diverse sources of scientific knowledge with personal experiences, emotion and ethical judgments. In this paper, we assess the potential as well as the limitations of innovative theatre-based participatory tools and methods aimed at supporting sustainability learning and agent transformation. To this aim, we first review a series of experiences using theatrical performance and introduce the notion of performative methods. Second, we assess to what extent these new approaches can be of relevance in environmental action research and sustainability science, practice and learning. Finally, we list a series of key research questions to further guide methodological innovation in this promising area of sustainability science and practice. Our findings show a growing and successful use of such methodologies worldwide, both in academia and in implementation-oriented approaches. An increasing number of topics and complexity is being embraced by these methods, offering a fertile ground for innovation in participatory sustainability science.     

Evaluation of techniques used in the preparation of diesel extract samples for mutagenicity studies

Diesel exhaust particles were used to compare methods and techniques used in the preparation of particulate matter for microbial mutagenesis testing. Investigated in this study were extraction, concentration, and solvent exchange methodologies as they affected recovery of mutagenic material from diesel samples using a Salmonella typhimurium plate incorporation assay. Solvent removal methods applicable for use in determining the mass concentration of extracts were also evaluated. Results indicated that particle samples Soxhlet extracted with dichloromethane yielded higher levels of mutagenic activity than did comparative samples utilizing sonication. No difference was seen between rotary evaporation or Kuderna-Danish macro concentration of extracts to volumes > 50 mL. In comparison of micro concentration techniques to volumes < 10 mL, vortex evaporation was found to be more efficient than a modified micro Kuderna-Danish method in recovery of mass and mutagenicity. Solvent exchanged samples were found to yield higher recoveries of mutagenic activity than samples taken to dryness and then reconstituted in the bioassay solvent. A dry mass weighing procedure utilizing desiccation was found to be more acceptable than either the use of an infrared heat lamp or nitrogen blowdown for solvent removal.