宁夏乌拉尔甘草地上资源的开发利用

报道了系统提取、分离宁夏乌拉尔甘草地上部分的色素类和生物黄酮类等有效成分的工艺及产品开发前景展望.最佳工艺是:料叶用95%的乙醇浸泡后,将色素类和黄酮类用石油醚分离,色素类可以制成叶绿素铜钠盐及叶黄素等,黄酮通过柱层析法制得含量大于80%的甘草叶黄酮产品.该工艺操作简单、连续,可以实现工业化生产.     

菝葜乙醇提取物的抑菌作用

用不同浓度的菝葜乙醇提取物对4种细菌、1种酵母菌和1种霉菌进行抑菌实验。结果表明:菝葜乙醇提取物对金黄色葡萄球菌、苏云金芽孢杆菌、大肠杆菌和枯草芽孢杆菌的最低抑菌浓度分别为0 14、0 18、0 46和0 44(g/12ml培养基),菝葜乙醇提取物对产黄青霉和啤酒酵母的生长无明显抑制作用。研究结果对进一步综合开发利用菝葜植物资源具有重要意义。     

重庆华蓥山山脉菝葜属药用植物资源调查

经调查鉴定,重庆华蓥山山脉有菝葜属药用植物7种,其中菝葜、小叶菝葜、光叶菝葜分布最广.重点介绍了它们的医疗用途、地理分布、资源状况及其开发前景,为更好地开发利用华蓥山山脉菝葜属药用植物资源提供科学依据.     

花生壳中黄酮物质的提取及对油脂的抗氧化作用

对花生壳中黄酮物质的提取工艺进行了优化研究,并对提取物的抗氧化性进行了初步研究。用单因素试验分别对提取溶剂乙醇体积分数、料液比、提取时间及提取温度进行了研究。在此基础上,通过正交试验确定上述4个因素的最佳组合。优化后花生壳中黄酮的最大提取率为92．32％,花生壳中黄酮类化合物的适宜提取条件为乙醇体积分数60％、料液比1：30、提取时间150min、提取温度60℃。花生壳中黄酮类化合物对猪油有明显的抗氧化作用,当添加量为0．2％时抗氧化效果与0．2％BHT接近。     

重庆华蓥山山脉菝葜属药甩植物资源调查

经调查鉴定,重庆华蓥山山脉有菝葜属药用植物7种,其中菝葜、小叶菝葜、光叶菝葜分布最广。重点介绍了它们的医疗用途、地理分布、资源状况及其开发前景,为更好地开发利用华蓥山山脉菝葜属药用植物资源提供科学依据。     

菝葜水煎液抗炎活性的实验研究

目的：探讨菝葜水煎液的抗炎活性。方法：利用巴豆油合剂致小鼠耳肿胀、醋酸致小鼠胸膜炎和皮下植入棉球致小鼠生长肉芽等3个实验模型研究菝葜水煎液的抗炎活性。结果：菝葜水煎液对巴豆油合剂引起的小鼠耳肿胀具有显著的抑制作用，而对醋酸引起的小鼠胸膜炎和皮下棉球引起的小鼠肉芽肿则仅有一定的抑制作用。结论：菝葜水煎液具有一定的抗炎活性。     

星点设计——响应面法优化葎草黄酮的提取工艺

为了探究并优化葎草中黄酮的提取工艺,在单因素实验的基础上,采用星点设计-响应面法设计实验,最终确定了葎草黄酮的最优提取工艺.回归分析和实验结果表明,最佳提取工艺为:乙醇浓度=72％、料液比(W/V)=1∶28、提取时间=1.2h,实际黄酮/生药提取率为2.64235.该方法优于现有的提取方法,得到的工艺条件具有实际应用价值.     

黑木耳和茶叶黄酮复配物的抗氧化活性

研究了黑木耳和茶叶黄酮复合对两种自由基体系的活性清除。结果表明,黑木耳和茶叶黄酮复合对羟基自由基（OH.）和超氧阴离子自由基（O2-.）均有较强的清除作用,且随着用量的增加清除效果增强。在同样用量的情况下,其清除作用明显强于单种黄酮的抗氧化作用,初步说明两种黄酮的复合可增强清除自由基的活性。     

树舌灵芝菌丝体不皂化物GC-MS分析

在树舌灵芝菌丝体化学成分研究中,采用乙醚抽提法获取树舌灵芝菌丝体脂溶性物质,经皂化后使用气相色谱-质谱联用技术(GC-MS)进行分析,共鉴定出19种物质,所得到的丁羟甲苯、乙酰菲、5,6-二氢麦角甾醇对树舌灵芝菌丝体化学成分研究具有较高价值.     

有机废水资源化技术--发酵产氢

氢作为一种清洁高效的可再生能源日益受到人们的重视.利用有机废水发酵产氢,既保护环境又可获得氢能,是一条符合可持续发展战略的废水资源化途径,极具发展前景.本文介绍了发酵产氢微生物的种类及其产氢机理,对发酵产氢的影响因素进行了分析;总结了有机废水发酵产氢的研究现状,并探讨了今后的研究方向.     

Root penetration of sealing layers made of fly ash and sewage sludge

Fly ash and sewage sludge are suggested materials for constructing sealing layers covering mine tailings impoundments. Little is known, however, of their effect on vegetation or resistance to root penetration. We investigate: (i) the ability of different plant species to grow in sealing layers comprising fly ash and sewage sludge, (ii) the impact on plant growth of freshly hardened fly ash compared to aged and leached ash, and (iii) the plant stress response to fly ashes of different properties. A 6-mo greenhouse study using birch (Betula pendula Roth.), Scots pine (Pinus sylvestris L.), Kentucky bluegrass (Poa pratensis L.), and willow (Salix viminalis L.) demonstrated that no roots could grow into a compacted layer consisting only of ash, while a 6:4, ash-sludge mixture admitted roots into the upper part and a 1:9, ash-sludge mixture was totally penetrated (to 15 cm in depth) by roots of willow and Scots pine. Freshly hardened ash prevented root growth more effectively than aged ash did, as was observed in tests using reed canarygrass (Phalaris arundinacea L.) and pea (Pisum sativum L.). Furthermore, extracts of highly alkaline ash were more toxic to pea in a 48-h toxicity test than less alkaline ash was. However, stress responses to diluted ash extracts of lower pH, measured as enzyme capacities in dwarf bean (Phaseolus vulgaris L.), were more related to the metal and ion contents. Root penetration of sealing layers is most effectively prevented if little sewage sludge is added, and if ash of high alkalinity is chosen.     

The role of nitrilotriacetate in copper uptake by tobacco

In growth chamber experiments we studied the effect of nitrilotriacetate (NTA) on Cu uptake by tobacco (Nicotiana tabacum L.). Plants were exposed for 6 d to 126 microM Cu and 500 microM NTA in nutrient solutions without and with 10 g L(-1) montmorillonite. Approximately seven times less Cu was dissolved in the montmorillonite solutions than in the nutrient solutions alone. In the absence of NTA, montmorillonite effectively competed with plant roots for Cu, although Cu remained bound to the roots. Nitrilotriacetate increased Cu uptake and translocation into shoots of tobacco by a factor of 3.5 from the nutrient solution and by a factor of 26 from the montmorillonite nutrient solution. Neither growth reduction nor any other visible sign of Cu toxicity was found in the presence of NTA with Cu concentrations of 190 mg kg(-1) in the shoots. In the absence of NTA, high Cu concentrations in root samples led to a brownish discoloration of the roots.     

Infrared monitoring of dinitrotoluenes in sunflower and maize roots

Infrared microspectroscopy (IMS) is emerging as an important analytical tool for the structural analysis of biological tissue. This report describes the use of IMS coupled to a synchrotron source combined with principal components analysis (PCA) to monitor the fate and effect of dinitrotoluenes in the roots of maize and sunflower plants. Infrared imaging revealed that maize roots metabolized 2,4-dinitrotoluene (DNT) and 2,6-DNT. The DNTs and their derivative aromatic amines were predominantly associated with epidermis and xylem. Both isomers of DNT altered the structure and production of pectin and pectic polysaccharides in maize and sunflower plant roots. Infrared peaks diagnostic for aromatic amines were seen at the 5 mg L concentrations for both DNTs in maize and sunflower treated tissue. However, only infrared peaks for nitro groups, not aromatic amines, were present in the maize treated at 10 mg L For sunflower, the 10 mg L level was toxic and also produced very dark root systems making spectra difficult to obtain. Maize and sunflower seem unable to metabolize effectively at concentrations higher than about 5 mg L DNT in hydroponic solution. Based on the results of this study, IMS combined with PCA can be an effective means of determining the fate and metabolism of organic contaminants in plant tissue when isotopically labeled compounds are not available.     

Particulates, not plants, dominate nitrogen processing in a septage-treating aerated pond system

In pond and wetland systems for wastewater treatment, plants are often thought to enhance the removal of ammonium and nitrogen through the activities of root-associated bacteria. In this study, we examined the role of plant roots in an aerated pond system with floating plants designed to treat high-strength septage wastewater. We performed both laboratory and full-scale experiments to test the effect of different plant root to septage ratios on nitrification and denitrification, and measured the abundances of nitrifying bacteria associated with roots and septage particulates. Root-associated nitrifying bacteria did not play a significant role in ammonium and total nitrogen removal. Investigations of nitrifier populations showed that only 10% were associated with water hyacinth [Eichhornia crassipes (Mart.) Solms] roots (at standard facility plant densities equivalent to 2.2 wet g roots L(-1) septage); instead, nitrifiers were found almost entirely (90%) associated with suspended septage particulates. The role of root-associated nitrifiers in nitrification was examined in laboratory batch experiments where high plant root concentrations (7.4 wet g L(-1), representing a 38% net increase in total nitrifier populations over plant-free controls) yielded a corresponding increase (55%) in the non-substrate-limited nitrification rate (V(max)). However, within the full-scale septage-treating pond system, nitrification and denitrification rates remained unchanged when plant root concentrations were increased to 7.1 g roots L(-1) (achieved by increasing the surface area available for plants while maintaining the same tank volume). Under normal facility operating conditions, nitrification was limited by ammonium concentration, not nitrifier availability. Maximizing plant root concentrations was found to be an inefficient mechanism for increasing nitrification in organic particulate-rich wastewaters such as septage.     

"三个代表"重要思想在党的自身建设中的作用

在"三个代表"重要思想的指导和引领下如何加强党的建设的基本途径,即必须加强党的基层组织建设,夯实党的根基;必须加强干部队伍建设,保证干部队伍素质;必须加强党员队伍建设,使党员成为带领群众前进的先锋模范.     

Polyphenol oxidase activity in subcellular fractions of tall fescue contaminated by polycyclic aromatic hydrocarbons

Understanding enzyme responses to contamination with persistent organic pollutants (POPs) is a key step in the elucidation of POP metabolic mechanisms in plants. However, there is little information available on enzyme activity in subcellular fractions of POP-contaminated plants. To our knowledge, this is the first study to investigate the activities of polyphenol oxidase (PPO) in cell fractions of plants under contamination stress from polycyclic aromatic hydrocarbons (PAHs) using a greenhouse batch technique. Three parameters, E(cell), E(cell-n), and P(cell), denoting the amount of PPO activity, cell fraction content-normalized PPO activity, and proportion of PPO activity in each cell fraction, respectively, were used in this study. Contamination with phenanthrene, as a representative PAH, at a relatively high level (>0.23 mg L?1) in culture solution generally stimulated PPO activity in tall fescue (Festuca arundinacea Schreb.) roots and shoots and their cellular fractions. The amount and distribution proportion of PPO activity in each cell fraction of phenanthrene-contaminated roots and shoots were (in descending order): cell solution > > cell wall > cell organelles. Cell solution was the dominant storage domain of PPO activity and contributed 84.0 and 82.8% of PPO activity in roots and shoots, respectively. The cell wall had the highest density of PPO activity in roots and shoots, based on the highest cell fraction content normalized PPO activity in this cell fraction. Our results provide new information on enzyme responses in plant intracellular fractions to xenobiotic POPs and fundamental information on within-plant POP metabolic mechanisms.     

Influence of decomposition on chemical properties of plant- and manure-derived dissolved organic matter and sorption to goethite

Sorption of dissolved organic matter (DOM) plays an important role in maintaining the fertility and quality of soils in agricultural ecosystems. Few studies have examined the effects of decomposition on DOM sorption and chemical characteristics. This study investigated the sorption to goethite (alpha-FeOOH) of fresh and decomposed hydrophilic (HPL) and hydrophobic (HPB) DOM fractions extracted from the shoots and roots of crimson clover (Trifolium incarnatum L.), corn (Zea mays L.), soybean [Glycine max (L.) Merr.], hairy vetch (Vicia villosa L.), and dairy and poultry manures. Sorption was positively related to apparent molecular weight (MWAP), aromaticity as measured by absorptivity at 280 nm, and phenolic acid content. A 10-d laboratory microbial decomposition of the source organic matter generally increased the sorption of the extracted DOM onto goethite. The decomposition effect on sorption was greater for the HPL fractions than for the HPB fractions. There was a decrease in the MWAP values of the DOM samples following sorption to goethite. In many cases the reduction in MWAP was large, indicating a strong preference by goethite for the higher MWAP DOM fractions. The results of this laboratory-based research demonstrate that microbial processes affect the chemical characteristics of DOM which may affect the distribution of soil organic C pools.     

丛枝菌根在矿区生态重建中的应用及其前景分析

目前国内外矿山复垦研究的新热点是应用现代微生物工艺技术加速新覆盖土的改性与熟化。在总结分析国内外微生物复垦技术研究现状的基础上,对丛枝菌根应用于矿区复垦的前景进行了展望。加快这项技术的应用研究,对拓宽我国科学复垦技术研究领域、提高土地复垦率具有重要意义。