A novel role of computers in the natural products field

We describe several computer programs developed in our laboratory as aids in the determination of unknown molecular structures. These programs include CONGEN [1], for determination of all plausible structural candidates; REACT [2, 3], for simulation of sequences of chemical reactions; and STRUCC, for evaluating candidates and planning new experiments. We stress the importance of symbiosis between chemist and computer, the combination building on their respective strengths to increase the speed and accuracy of structural assignments.     

Multicomponent floral signals elicit selective foraging in bumblebees

Flower constancy, or the tendency of individual pollinators to visit sequentially a single flower type even when other equally rewarding types are available, has important implications for animal-pollinated plants. Yet, the proximal reason for the behaviour still remains poorly understood. Here I show that bumblebees visiting equally rewarding flowers that differ in size and odour are more flower constant and less efficient (visited fewer flowers per minute) than bees visiting flowers that differ in size only and odour only. These results are consistent with the view that flower constancy in pollinators is related to their inability to perceive, process or recall multicomponent floral signals. I discuss these findings in the context of pollinator behavioural mechanisms and the evolution of floral diversity.     

A risk assessment system for alien plant bio-invasion in Xiamen of China

Bio-invasion has caused serious ecosystem damage and enormous economic losses in China, and it has been the greatest factor to island bio-diversity reduction. Xiamen, as an important seaport with a relatively high conservation value, is particularly vulnerable to bio-invasion for its typical island ecosystem as well as frequent human-made disturbance. As a result of field surveys, literature review, and consultation with experts, a list of 67 alien plants identified as major invaders (12 species) and emerging invaders (55 species) in Xiamen has been compiled. Based on the analysis of the current situation of bio-invasion in Xiamen, a risk assessment system for alien plant invasion has been designed using a ranking system and an analytic hierarchy process. The system consists of 17 secondary indices, grouped into 6 primary indices reflecting the different stages in the bio-invasion process: introduction, establishment, dispersion, current range, infestation, and artificial control. Biogeographical, ecological, and experience-linked aspects of the species as well as artificial disturbance were taken into account in the index selection and criterion development. The system was then validated (and worked well) using fifty well-known alien plant species as candidates. Appropriate recommendations are proposed to help local policy-makers prioritize their decisions on such alien plants.     

A prototype of proposed treatment plant for sago factory effluent

Sago processing industries discharge wastewater, which poses a threat to water bodies due to their higher organic loading. Concerns about pollution related problems in the global scenario are persuading all the processing industries to adopt cleaner manufacturing practices. Hence, this study was aimed at developing a cost-effective and eco-friendly treatment plant based on microbial system to treat the sago factory effluent effectively in order to reuse the water for industrial purposes. The experimental results showed that, anaerobic treatment with mixed inoculum for 10 days followed by 6 days of fungal treatment was found to be effective in effluent treatment. The fusant showed a higher efficiency in the treatment of sago factory effluent than the parental strains.     

A novel gas purification system for biologically produced gases

A novel gas purification system employing membrane contactors with dense membranes in the absorption as well as the desorption unit has been tested concerning the separation efficiency of CO₂. The separation efficiency of potassium carbonate (K₂CO₃) promoted by piperazine (PZ) as absorption medium has been tested in the absorption unit. Since the absorbent will be regenerated in the desorption unit, the influence of temperature, sweep flow rate and absorbent type (0.5 M K₂CO₃ and 0.5 M K₂CO₃/0.5 M PZ) on the separation efficiency of CO₂ in the desorption unit has been investigated. Desorption by vacuum has been considered as well.In addition experimental results of the coupling of a bioreactor producing hydrogen by anoxygenic photosynthesis (low and fluctuating product gas flow rates and fluctuating compositions) with a membrane contactor for gas purification are reported. This was done in order to show the applicability of this system to processes producing biogases.     

A 50 MW concentrating solar power plant for Jordan

The potential of concentrating solar power plant (CSPP) technology in Jordan is assessed and the next steps for development of the first CSPP in the country are presented. For this purpose, a prototype of a 50 MW CSPP for electricity generation in Jordan is proposed and analysis of its economic feasibility has been performed. Moreover, a calculation model – using the concept design of the proposed CSPP, and the solar irradiation data – was developed to estimate the energy yield of the plant. Based on the analyzed solar irradiation data, it was found that Jordan has an outstanding potential for CSPP, especially in the southern locations of the country such as Quweira. At the same time Jordan's energy master plan is seeking for independent energy supply and for an increasing share of renewable energies. Furthermore, Jordan's demand curve over a day closely matches the electricity production of the proposed plant. Combination of these factors creates a very favorable situation. Therefore, establishment of the proposed 50 MW CSPP is highly recommended not only in Jordan, but also in many other countries, which have similar political and economical conditions such as those countries located in the Middle East and North Africa (MENA) region.     

A novel marine silk

The discovery of a novel silk production system in a marine amphipod provides insights into the wider potential of natural silks. The tube-building corophioid amphipod Crassicorophium bonellii produces from its legs fibrous, adhesive underwater threads that combine barnacle cement biology with aspects of spider silk thread extrusion spinning. We characterised the filamentous silk as a mixture of mucopolysaccharides and protein deriving from glands representing two distinct types. The carbohydrate and protein silk secretion is dominated by complex β-sheet structures and a high content of charged amino acid residues. The filamentous secretion product exits the gland through a pore near the tip of the secretory leg after having moved through a duct, which subdivides into several small ductules all terminating in a spindle-shaped chamber. This chamber communicates with the exterior and may be considered the silk reservoir and processing/mixing space, in which the silk is mechanically and potentially chemically altered and becomes fibrous. We assert that further study of this probably independently evolved, marine arthropod silk processing and secretion system can provide not only important insights into the more complex arachnid and insect silks but also into crustacean adhesion cements.     

The role of plant leaf attributes in linking land use to ecosystem function in temperate grassy vegetation

We tested predictions of the relative changes in plant leaf traits in response to land uses in Australian eucalypt grassy ecosystems. Predictions were determined from responses observed in European landscapes in relation to disturbances associated with agricultural land uses. We measured specific leaf area (SLA) and leaf dry matter content (LDMC) across five land uses: reference sites (closest to pre-European state), native pastures (unfertilized), fertilized pastures, sown pastures (cultivated and fertilized) and enriched grassland (previously fertilized, no longer grazed). Leaves were expected to have higher SLA and lower LMDC at sites with increasing fertility and/or disturbance.The predictions were confirmed, with SLA increasing progressively in land uses associated with (1) grazing; (2) grazing and fertilization; (3) grazing, fertilization and cultivation. Values for LDMC were closely (but inversely) correlated with those of SLA. For both traits, there were relationships with available soil phosphorus but not with soil total nitrogen. The positive correlation of SLA with phosphorus was not evident above 30 mg kg⁻¹, the recommended level of phosphorus for improved pastures.Results confirm patterns of leaf-trait response to disturbance that reflect fundamental constraints to plant survival in habitats with different levels of resources and disturbances. A conservative strategy for low productivity undisturbed habitats is associated with low SLA and high dry matter content in contrast to fertile disturbed habitats which select for high SLA and low dry matter content. The changes in leaf traits across land uses resulted from species substitution rather than variation within species across sites, and most notably the replacement of native by annual exotic species as land use intensifies.Recommended fertilization rates for pasture production convert the ground layer to plants with soft, digestible leaves, that are responsive to fertilizer and desirable for livestock production. However, fertilization also drastically reduces the diversity of native plants and annual plants tend to dominate. The trade-off associated with high production includes increased vulnerability to soil erosion, due to reduced plant cover and low persistence of cover. If alternative ecosystem values such as erosion control, water quality, salinity control and biodiversity persistence are required, incentives may be needed to offset the loss of production that can be gained from fertilizer application.     

The role of tending ants in host plant selection and egg parasitism of two facultative myrmecophilous butterflies

Ovipositing adult females of myrmecophilous lycaenids are expected to select plants based on ant presence in order to maximize the survivorship of immature stages. Usually, larvae feed ants with honey-like solutions and, in turn, ants ward off parasitoids. Nonetheless, a rarely investigated approach is whether ant partners can also extend their protective behavior towards lycaenids eggs. Here, we investigated the ant-related oviposition pattern of Allosmaitia strophius and Rekoa marius; then, we compared egg parasitism according to the presence of ants. Lycaenid oviposition and egg parasitism (in percent) were experimentally compared in ant-present and ant-excluded treatments. The study plant, Heteropterys byrsonimifolia, is an extrafloral nectaried shrub which supports several ant species. We sampled 280 eggs, of which 39.65 % belonged to A. strophius and 60.35 % to R. marius. Both lycaenids eggs were significantly more abundant on branches with ants, especially those with Camponotus crassus and Camponotus blandus, two ant species known to attend to lycaenids. A. strophius and R. marius parasitism was 4.5- and 2.4-fold higher, respectively, in ant-present treatments, but the results were not statistically significant. Our study shows that ant-mediated host plant selection in lycaenids might be much more widespread than previously thought, and not restricted to obligate myrmecophilous species. Tending ants may be inefficient bodyguards of lycaenid eggs, because unlike larvae which release sugared liquids, eggs do not offer obvious rewards to ants. Ants can ward off parasitoids of larvae, as observed elsewhere, but our findings show that positive ant–lycaenid interactions are conditional and depend on immature ontogeny.     

A novel approach of preparing press-powders for cleaner production of ceramic tiles

China produces almost half of ceramic tiles in the world. However, serious air pollution and high energy consumption in the preparation of ceramic press-powders by traditional wet process (WP) have become major barriers for the sustainable development of ceramic tile manufacturing industry. A novel cleaner production process, named by the authors as Droplet-Powder Granulation Process (DPGP), was proposed and tested in this work. Droplet-powder granulator, a key facility of DPGP, was designed. In the granulator, ceramic suspension was directly sprayed to form fine suspension droplets and ceramic dry powder was simultaneously jetted and dispersed to enwrap, insulate and adsorb the suspension droplets so as to form coarse granules that were then rolled, tamped and dried into press-powders. Lab experiments of preparing press-powders by DPGP were carried out. The as-prepared DPGP press-powders consist of solid granules with nearly spherical shape and approximately normal grain size distribution, and present favorable flowability and relatively high bulk density, with the similar properties of the press-powders as those prepared by WP. Moreover, as compared with WP, DPGP can reduce both pollutant emission of 98% dust, 65% sulfur oxides and 71% nitrogen oxides and resource consumption of 24% energy and 69% water. Therefore, DPGP is an innovative approach for cleaner production of ceramic press-powders.