Heavy metal and selenium levels in feathers of herring gulls (Larus argentatus): Differences due to year,gender, and age at Captree,Long Island

The concentrations of heavy metals (mercury, lead, cadmium, chromium, manganese) and selenium in the feathers of herring gulls (Larus argentatus) from a nesting colony at Captree, Long Island, New York were examined from 1989 to 1993 to determine if there were differences from year to year, and between males and females, adult and young, and dead versus live gulls. Variation in metal levels in regression models was explained by age (all metals), year (all except manganese), and whether the feathers were from live or dead birds (all except lead and chromium). The feathers of adults had significantly higher levels of mercury, lead and manganese than those of young, but lower levels of selenium and cadmium than those of young. Levels in down and fledgling feathers were similar for lead, cadmium and selenium, but fledgling feathers had higher levels for mercury, chromium, and manganese. There were no gender differences in metal levels for adult feathers except for lead (females had higher levels). Levels of mercury and manganese were higher in feathers of live adults whereas levels of cadmium and selenium were higher in the feathers of dead adults.     

Heavy metal and selenium levels in birds at Agassiz National Wildlife Refuge,Minnesota: Food chain differences

The levels of heavy metals and selenium in the eggs and in breast feathers of adult doublecrested cormorant (Phalacrocorax auritus), black-crowned night heron (Nycticorax nycticorax), and franklin's gull (Larus pipixcan) nesting at Agassiz National Wildlife Refuge in Marshall County, northwestern Minnesota were examined. Also examined were metal levels in the feathers of fledgling night herons and gulls, in the feathers of adult and fledgling American bittern (Botaurus lentiginosus), in eggs of American coot (Fulica americana) and eared grebe (Podiceps caspicus), and in feathers of adult Canada geese (Branta canadensis). These species represent different levels on the food chain from primarily vegetation-eating species (geese, coot) to species that eat primarily fish (cormorant). A clear, positive relationship between level on the food chain and levels of heavy metals occurred only for mercury in feathers and eggs. Otherwise, eared grebes had the highest levels of all other metals in their eggs compared to the other species. No clear food chain pattern existed for feathers for the other metals. For eggs at Agassiz: 1) lead, selenium, and manganese levels were similar to those reported in the literature, 2) mercury levels were slightly higher for cormorants and night herons, 3) all species had higher chromium and cadmium levels than generally reported, and 4) eared grebes had significantly higher levels of cadmium than reported for any species from elsewhere. For adult feathers: 1) gulls had higher levels of lead than the other species, 2) cadmium levels were elevated in gulls and adult herons and cormorants, 3) mercury levels showed an increase with position on the food chain, 4) selenium and chromium levels of all birds at Agassiz were generally low and 5) manganese levels in adults were generally higher than in the literature for other species. Adults had significantly higher mercury levels than fledgling gulls, night herons, and bitterns.     

Mercury and other metals in eggs and feathers of glaucous-winged gulls (Larus glaucescens) in the Aleutians

Levels of mercury and other contaminants should be lower in birds nesting on isolated oceanic islands and at high latitudes without any local or regional sources of contamination, compared to more urban and industrialized temperate regions. We examined concentrations of arsenic, cadmium, chromium, lead, manganese, mercury and selenium in the eggs, and the feathers of fledgling and adult glaucous-winged gulls (Larus glaucescens) nesting in breeding colonies on Adak, Amchitka, and Kiska Islands in the Aleutian Chain of Alaska in the Bering Sea/North Pacific. We tested the following null hypotheses: 1) There were no differences in metal levels among eggs and feathers of adult and fledgling glaucous-winged gulls, 2) There were no differences in metal levels among gulls nesting near the three underground nuclear test sites (Long Shot 1965, Milrow 1969, Cannikin 1971) on Amchitka, 3) There were no differences in metal levels among the three islands, and 4) There were no gender-related differences in metal levels. All four null hypotheses were rejected at the 0.05 level, although there were few differences among the three test sites on Amchitka. Eggs had the lowest levels of cadmium, lead, and mercury, and the feathers of adults had the lowest levels of selenium. Comparing only adults and fledglings, adults had higher levels of cadmium, chromium, lead and mercury, and fledglings had higher levels of arsenic, manganese and selenium. There were few consistent interisland differences, although levels were generally lower for eggs and feathers from gulls on Amchitka compared to the other islands. Arsenic was higher in both adult feathers and eggs from Amchitka compared to Adak, and chromium and lead were higher in adult feathers and eggs from Adak compared to Amchitka. Mercury and arsenic, and chromium and manganese levels were significantly correlated in the feathers of both adult and fledgling gulls. The feathers of males had significantly higher levels of chromium and manganese than did females. The levels of most metals in feathers are below those known to be associated with adverse effects in the gulls or their predators. However, levels of mercury in some gull eggs are within a range suggesting that several eggs should not be eaten in one day by sensitive humans.     

Assessment of metals in down feathers of female common eiders and their eggs from the Aleutians: arsenic, cadmium, chromium, lead, manganese, mercury, and selenium

Concentrations of arsenic, cadmium, chromium, lead, manganese, mercury and selenium were examined in the down feathers and eggs of female common eiders (Somateria mollissima) from Amchitka and Kiska Islands in the Aleutian Chain of Alaska to determine whether there were (1) differences between levels in feathers and eggs, (2) differences between the two islands, (3) positive correlations between metal levels in females and their eggs, and (4) whether there was more variation within or among clutches. Mean levels in eggs (dry weight) were as follows: arsenic (769 ppb, ng/g), cadmium (1.49 ppb), chromium (414 ppb), lead (306 ppb), manganese (1,470 ppb), mercury (431 ppb) and selenium (1,730 ppb). Levels of arsenic were higher in eggs, while chromium, lead, manganese, and mercury were higher in feathers; there were no differences for selenium. There were no significant interisland differences in female feather levels, except for manganese (eider feathers from Amchitka were four times higher than feathers from Kiska). Levels of manganese in eggs were also higher from Amchitka than Kiska, and eider eggs from Kiska had significantly higher levels of arsenic, but lower levels of selenium. There were no significant correlations between the levels of any metals in down feathers of females and in their eggs. The levels of mercury in eggs were below ecological benchmark levels, and were below human health risk levels. However, Aleuts can seasonally consume several meals of bird eggs a week, suggesting cause for concern for sensitive (pregnant) women.     

Heavy metals and selenium in feathers of three shorebird species from Delaware bay

Concentrations of lead, cadmium, mercury, selenium, chromium and manganese were examined in breast feathers of shorebirds migrating north through Cape May, New Jersey in 1991 and 1992. Although we predicted that metal levels would be positively correlated with weight, this was only true for mercury in red knots (Calidris canutus). Selenium was negatively correlated with weight in red knots. No other significant correlation of metal concentrations with weight were found. Lead and mercury were highest in sanderlings (C. alba). Selenium and manganese were highest in red knots, while chromium and cadmium levels were highest in semipalmated sandpipers (C. pusilus). For 1991, interspecific metals differences were significant for all metals except lead. For semipalmated sandpipers, cadmium and chromium concentrations were significantly higher in 1991 while managese concentrations were significantly higher in 1992.     

Heavy metal concentrations in feathers of common loons (Gavia immer) in the Northeastern United States and age differences in mercury levels

Feathers serve as a useful, non-destructive approach for biomonitoring some aspects of environmental quality. Birds can eliminate over 90% of their body burden of mercury by sequestration in growing feathers, and they molt their feathers at least annually. Thus mercury concentrations should not vary in avian feathers as a function of age. We tested the null hypothesis that there are no age differences in the concentrations of mercury, lead, cadmium, selenium, copper, chromium and manganese in the feathers of immature and adult common loons Gavia immer from the Northeastern United States where the species is declining. Adults had significantly higher mean levels of mercury (20245 ppb) than immature loons (9677 ppb), but there were no age-related differences for other elements. Even with the small number of immatures, females had significantly higher levels of mercury than males, although the gender difference was not significant for adults.     

Determination of lead, cadmium and copper in roadside soil and plants in Elazig, Turkey

The concentrations of lead, cadmium and copper in roadside soil and plants in Elazig, Turkey were investigated. Soil samples were collected at distances of 0, 25 and 50 m from the roadside. The concentrations of lead, cadmium and copper were measured by Flame Atomic Absorption Spectrophotometry (FAAS). A slotted tube atom trap (STAT) was used to increase the sensitivity of lead and cadmium in FAAS. Lead concentrations in soil samples varied from 1.3 to 45 mg kg⁻¹ while mean lead levels in plants ranged from120 ng g⁻¹ for grape in point-4 to 866 ng g⁻¹ for apple leaves in point-2. Lead analyses showed that there was a considerable contamination in both soil and plants affected from traffic intensity. Overall level of Cd in soil samples lies between 78 and 527 ng/g while cadmium concentration in different vegetations varied in the range of 0.8–98.0 ng g⁻¹. Concentrations of copper in soil and plant samples were found in the range of 11.1–27.9 mg kg⁻¹ for soil and 0.8–5.6 mg kg⁻¹ for plants. Standard reference material (SRM) was used to find the accuracy of the results of soil analyses.     

Particle-phase Polycyclic Aromatic Hydrocarbon Emissions from Non-catalysed, In-use Four-stroke Scooters

Metals and radionuclide levels in marine birds of the Aleutians are of interest because they are part of subsistence diets of the Aleut people, and can also serve as indicators of marine pollution. We examined geographic and species-specific variations in concentrations of radionuclides in birds and their eggs from Amchitka, the site of underground nuclear tests from 1965 to 1971, and Kiska Islands (a reference site) in the Aleutians, and the levels of lead, mercury and cadmium in eggs. In 2004 we collected common eiders (Somateria mollissima), tufted puffins (Fratercula cirrhata), pigeon guillemot (Cepphus columba) and glaucous-winged gulls (Larus glaucescens) from Amchitka and Kiska, and eggs from eiders and gulls from the two island. We also collected one runt bald eagle (Haliaeetus leucocephalus) chick from both Amchitka and Kiska Islands. For most species, the levels of radionuclide isotopes were below the minimum detectable activity levels (MDA). Out of 74 cesium-137 analyses, only one composite (gulls) was above the MDA, and out of 14 composites tested for plutonium (Pu-239, 240), only one exceeded the MDA (a guillemots). Three composites out of 14 tested had detectable uranium-238. In all cases, the levels were low and close to the MDAs, and were below those reported for other seabirds. There were significant interspecific differences in metal levels in eggs: gulls had significantly higher levels of cadmium and mercury than the eiders, and eiders had higher levels of lead than gulls. There were few significant differences as a function of island, but eiders had significantly higher levels of cadmium in eggs from Kiska, and gulls had significantly higher levels of mercury on Kiska. The levels of cadmium and mercury in eggs of eiders and gulls from this study were above the median for cadmium and mercury from studies in the literature. The levels of mercury in eggs are within the range known to affect avian predators, but seabirds seem less vulnerable to mercury than other birds. However, the levels of mercury are within the action levels for humans, suggesting some cause for concern if subsistence Aleuts eat a large quantity of eggs.     

Lead and cadmium contaminations in feathers of heron and egret chicks

In birds, concentrations of heavy metal contaminants were associated with their diet and environmental quality. This study presents concentrations of lead and cadmium in feathers and the relationship between concentrations in the feather and stomach content of three species of heron and egret chicks. Cadmium concentrations were higher in the Little Egret (geomean 0.75 μg/g dry weight) and Black-crowned Night Heron (geomean 0.88 μg/g dry weight) chicks than in Grey Heron chicks (geomean 0.48 μg/g dry weight). Lead and cadmium concentrations had significant correlations between feathers and stomach contents of heron and egret chicks. The mean lead and cadmium concentrations in feathers were within the background levels for wild birds. Heron and egret species forage around breeding colony (4–7 km). Lead and cadmium concentrations in heron and egret chicks were moderately associated with those of prey concentrations. These results suggest that contaminant concentrations such as lead and cadmium in feathers of heron and egret chicks can reflect local heavy metal contaminations and serve as a great biological indicator for monitoring heavy metal contamination. In addition, the feathers can be a routine monitoring tool over time and space without harming the wild birds.     

Metals in feathers of sooty tern, white tern, gray-backed tern, and brown noddy from islands in the North Pacific

Levels of heavy metals are usuallyhigher in adult than young birds because they eatlarger, more contaminated prey, or because they havehad longer to accumulate metals in their tissues.Further, levels of contaminants are usually less inbirds nesting on remote, offshore islands than inbirds breeding closer to mainland areas that areurbanized and industrialized. We examined the featherlevels of arsenic, cadmium, chromium, lead, manganese,mercury, and selenium in adult sooty terns (Sterna fuscata), gray-backed terns (Sterna lunata), and brown noddy (Anous stolidus, and adult and young white terns (Gygis alba, fromMidway Atoll, and in adult and young sooty terns andadult brown noddy from Manana Island, Hawaii (chicksof other terns were not available). We tested andrejected the null hypotheses that metal levels are notsignificantly different among species, ages, andlocations. Despite their small size, adult white ternshad the highest levels of lead, arsenic and tin. Brownnoddies had the highest levels of cadmium, chromium,manganese, and selenium. Sooty and white terns had thehighest levels of mercury. Sooty tern adults hadsignificantly higher levels of cadmium, mercury, andselenium than young, while young had significantlyhigher levels of arsenic and manganese. White ternadults had significantly higher levels of selenium andtin than young, while young had higher levels ofcadmium and mercury than adults. Except for mercury,there were significant inter-location differenceswithin species in all heavy metals. Contrary toexpectation, where the differences were great, metalsgenerally were higher in the feathers of terns andnoddies from Midway than from Manana.     

Metals in tern eggs in a New Jersey estuary: A decade of change

We compared levels of eight heavy metals in common tern eggs collected in 1982 (n=24) from coastal New Jersey with archived specimens collected from the same colonies in 1971 (N=9). Levels of all metals, except cobalt, showed a significant decrease over the 11 year period, with lead and nickel showing the greatest decline. Mercury levels declined over 50% based on geometric means. Cadmium and mercury showed the lowest levels in both years. Pair-wise correlation coefficients on log-transformed data revealed lower correlations in 1971, whereas most metal pairs showed significant correlations in 1982. Mercury levels showed significant positive correlations with cobalt, cadmium, and nickel in 1971, but were not correlated with levels of any other metal in 1982.     

Locational differences in metal concentrations in feathers of Australasian gannet (Morus serrator) in New Zealand

Levels of lead, cadmium, mercury, selenium, manganese and chromium were examined in breast feathers of Australasian Gannets (Morus serrator) from New Zealand waters, where they breed on coastal rocky stacks, islands, and occasionally coastal headlands. There were significant differences in the concentrations of all metals except mercury, with gannets from Ninety Mile Beach having significantly lower levels of all metals than those from the other beaches. Ninety Mile Beach is on the northern tip of the North Island, away from urbanization and industrialization, whereas the other beaches are closer to Auckland, the largest city in New Zealand. The levels of most metals were similar or lower than those reported for feathers from elsewhere around the world. Mercury, however, was at the high end, being exceeded only by raptors and gulls from industrialized countries. Several metal levels were highly correlated, indicating that gannets were usually exposed to several metals at once.     

The use of bioaccumulation, biomarkers and histopathology diseases in shape Procambarus clarkii to establish bioavailability of Cd and Zn after a mining spill

Individuals of the crayfish Procambarus clarkii (males and females) were exposed simultaneously to cadmium and zinc during 21 days. Exposure concentrations were those determined at the Guadiamar river after the Aznalcóllar mining spill (SW, Spain): 10 and 30 μg L⁻¹ of cadmium and 1000 and 3000 μg L⁻¹ of zinc. Three biomarkers (MT: metallothioneins like proteins, VTG: vitellogenin/vitellin like proteins and histopathology) together with heavy metal bioaccumulation were determined in soft tissues of male and female P. clarkii. At the concentrations tested, increasing cadmium exposure resulted in increasing cadmium bioaccumulation and increasing subletal effects (induction of MT, VTG and histopathological damage in tissues). Nevertheless, although increasing zinc exposure showed increasing VTG induction and histopathological damages, not a positive relationship was determined with MT induction. Concerning to responses determined in male and female crayfishes only differences were found between sexes at the highest cadmium exposure concentration related to bioaccumulation in hepatopancreas tissues. Biomarkers responses to heavy metal contamination in this crayfish, even VTG induction not before tested in heavy metal contamination assessment in crustaceans resulted potential tools for the monitoring of heavy metal environmental contamination.     

Assessment of trace metal concentration in feathers of seabird (Larus dominicanus) sampled in the Florianópolis, SC, Brazilian coast

Cd, Co, Cr, Cu, Mn, Ni, Zn, and Pb were measured in feather samples of adult, subadult, and juvenile of Larus dominicanus, sampled in the Florianólis, SC, in the south of Brazil in December 2005, by flame atomic absorption spectrophotometry. The average of the distribution of Cd concentration in adult feathers (0.072 μg g^???1) was significantly different than that found in juvenile feathers (0.021 μg g^???1). Cu concentration averages were not significantly different between adults (13.30 μg g^???1), subadults (9.67 μg g^???1), and juveniles (13.76 μg g^???1). For adults and juveniles there was significant difference in feather concentrations for Cd, Co, Cr, Ni, and Pb. The distribution of Mn concentration averages in feathers differs between adults (11.36 μg g^???1) and juveniles (1.184 μg g^???1). Ni concentration averages of adults (5.92 μg g^???1) were significantly higher than those of juveniles (2.23 μg g^???1). For Pb, concentration averages were significantly higher in adults (7.53 μg g^???1) than in juveniles (1.47 μg g^???1). The concentration of Co and Cr in juvenile and subadults are statistically different when compared with the adults. In the present study, levels of Cd, Co, Cr, Mn, Ni, Zn, and Pb increased with age. The concentrations of essential trace elements in L. dominicanus were generally comparable to values reported in other studies. With non-essential metals (Cd, Pb, and Ni), in our study, L. dominicanus had lower values than those reported for their northern Atlantic counterparts.     

Comparison of arsenic, cadmium, chromium, lead, manganese, mercury and selenium in feathers in bald eagle (Haliaeetus leucocephalus), and comparison with common eider (Somateria mollissima), glaucous-winged gull (Larus glaucescens), pigeon guillemot (Cepphus columba), and tufted puffin (Fratercula cirrhata) from the Aleutian Chain of Alaska

There is an abundance of field data for levels of metals from a range of places, but relatively few from the North Pacific Ocean and Bering Sea. In this paper we examine the levels of arsenic, cadmium, chromium, lead, manganese, mercury and selenium in feathers from common eiders (Somateria mollissima), glaucous-winged gulls (Larus glaucescens), pigeon guillemots (Cepphus columba), tufted puffins (Fratercula cirrhata) and bald eagles (Haliaeetus leucocephalus) from the Aleutian Chain of Alaska. Our primary objective was to test the hypothesis that there are no trophic levels relationships for arsenic, cadmium, chromium, lead, manganese, mercury and selenium among these five species of birds breeding in the marine environment of the Aleutians. There were significant interspecific differences in all metal levels. As predicted bald eagles had the highest levels of arsenic, chromium, lead, and manganese, but puffins had the highest levels of selenium, and pigeon guillemot had higher levels of mercury than eagles (although the differences were not significant). Common eiders, at the lowest trophic level had the lowest levels of some metals (chromium, mercury and selenium). However, eiders had higher levels than all other species (except eagles) for arsenic, cadmium, lead, and manganese. Levels of lead were higher in breast than in wing feathers of bald eagles. Except for lead, there were no significant differences in metal levels in feathers of bald eagles nesting on Adak and Amchitka Island; lead was higher on Adak than Amchitka. Eagle chicks tended to have lower levels of manganese than older eagles.     

Organochlorines and selenium in California night-heron and egret eggs

Exceptionally high concentrations of DDE were found in black-crowned night-heron (Nycticorax nycticorax) (geometric mean 8.62 g g^–1 wet wt.) and great egret (Casmerodius albus) (24.0 g g^–1) eggs collected from the Imperial Valley (Salton Sea), California in 1985. DDE concentrations in 14 of the 87 (16%) randomly selected night-heron eggs from six colonies (two in San Francisco Bay, three in the San Joaquin Valley, and one at Salton Sea) were higher than those associated with reduced reproductive success of night-herons (8 g g^–1). In addition, mean shell thickness of night-heron eggs collected from the San Joaquin Valley and from San Francisco Bay during 1982–1984 was significantly less than pre-DDT thickness and was negatively correlated (r=–0.50, n=75, P<0.0001) with DDE concentration. Mean selenium concentration in night-heron eggs from Salton Sea (1.10 g g^–1) was significantly higher than in eggs from three locations in the San Joaquin Valley, and in egret eggs from Salton Sea.     

Mercury, Methylmercury, and Selenium Concentrations in Eggs of Common Loons (Gavia immer) from Canada

Concentrations of Hg and Se were determined for a total of 125Common Loon (Gavia immer) eggs collected from lakes in Alberta, Saskatchewan, Ontario, Quebec, New Brunswick and NovaScotia, Canada between 1972 and 1997. Resulting data were compared to Hg and/or Se concentrations known or suspected tocause reproductive impairment in birds. Organic (methyl) Hg analyses were also performed on a subset of 24 loon eggs. Thirty-nine of 125 eggs had total Hg levels exceeding those (0.6 g g^-1 ww, or 2.5 g g^-1 dw)previously reported to be associated with reproductive impairment in common loons (Barr, 1986), and 9 of 125 eggshad Hg concentrations higher than the level associated withreproductive impairment in birds generally 1 g g^-1 ww; (Thompson, 1996). Selenium concentrations in loon egg samples were less than levels known to cause reproductiveimpairment in birds. A weak but significant positive correlation was observed between egg-Hg and -Se concentrations(r = 0.511, p < 0.05). On average, methylmercury accounted for about 87% of total Hg in 24 eggs analysed for both total and organic Hg. In this subset of eggs, the relationship between organic (methyl) Hg and Se was significant (r = 0.538, p = 0.007) while that found between inorganic Hg and Se in the same eggs was not significant (r = 0.353, p = 0.091). This relationship was unexpected and was contrary to relationships established for organic and inorganic Hg vs. Se in adult loon liver and kidney tissue (Scheuhammer et al., 1998b).     

Study of metals concentration levels in Patella piperata throughout the Canary Islands, Spain

In order to assess the extent of metal contamination at rocky shores of the Canarian Archipelago, metal concentrations have been measured in Patella piperata (Gould, 1846), using the standard atomic absorption spectrophotometer technique. Ranges of elements concentrations measured (in μg g⁻¹) found in the biota were: Cd (0.36 ± 0.26 μg g⁻¹ dry wt.), Cu (2.05 ± 0.91 dry wt.), Pb (1.57 ± 1.14 μg g⁻¹dry wt.) and Zn (10.37 ± 4.60 μg g⁻¹ dry wt.). Variation in metal concentrations in Patella, was tested by using non-parametric statistical methods. Cd content had a maximum in the Archipelago Chinijo, northward of Lanzarote Island. The metal concentrations recorded at the clean stations may be considered carefully if they are used like background levels.     

Bioindicator organisms: Heavy metal pollution evaluation in the Ionian Sea (Mediterranean Sea—Italy)

Trace metal concentrations of mercury, cadmium, lead and chromium in Patella caerulea, and Mullus barbatus were investigated to provide information on pollution of Ionian Sea, since these metals have the highest toxic potential. High chromium levels (0.47–0.97 g g^–1 ww) were registered in limpet samples collected from two station near the Gulf of Taranto, while elevated concentration of mercury (0.31–1.50 g g^–1 ww) were found in mullet specimens from Sicily. The metal concentrations recorded at the clean stations may be considered as useful background levels to which to refer for comparison within the Mediterranean area. On the contrary, the high levels of chromium and mercury found respectively in the areas near the Gulf of Taranto and at Capo Passero being of concern in terms of environmental health need frequent monitoring.     

Metal Levels in Raccoon Tissues: Differences On and Off the Department of Energy's Savannah River Site in South Carolina

Levels of arsenic, cadmium, chromium, copper, lead, manganese, selenium, and strontium⁸⁸ were examined in heart, kidney, muscle, spleen and liver of raccoons (Procyon lotor) from four areas on the Department of Energy's Savannah River Site (SRS), including near a former reactor cooling reservoir and a coal ash basin, and from public hunting areas within 15 km of the site. Mercury is mentioned briefly because it is discussed more fully in another paper. We test the hypotheses that there are no differences in metal levels between raccoons on SRS and off the SRS (off-site), and among different locations on the SRS. Although raccoons collected off-site had significantly lower levels of mercury and selenium in both the liver and kidney, there were few consistencies otherwise. There were significantly higher levels of cadmium in liver of on-site compared to off-site raccoons, and significantly higher levels of chromium and strontium⁸⁸ in kidney of on-site compared to off-site raccoons. Copper and manganese were highest in the liver; cadmium, lead, mercury and selenium were highest in the liver and kidney; chromium was highest in the spleen and muscle; arsenic was highest in the heart, and strontium⁸⁸ was slightly higher in the kidney than other organs. Where there were significant differences on site, chromium, manganese were highest in raccoon tissues from Steel Creek; arsenic, lead and selenium were highest in the Ash Basin; cadmium was highest at Upper Three Runs; and strontium⁸⁸ was highest at Upper Three Runs and Steel Creek. The patterns were far from consistent.