Acute O 3 damage on first year coppice sprouts of aspen and maple sprouts in an open-air experiment

We studied the effect of high ozone (O(3)) concentration (110-490 nmol mol(-1)) on regenerating aspen (Populus tremuloides) and maple (Acer saccharum) trees at an open-air O(3) pollution experiment near Rhinelander WI USA. This study is the first of its kind to examine the effects of acute O(3) exposure on aspen and maple sprouts after the parent trees, which were grown under elevated O(3) and/or CO(2) for 12 years, were harvested. Acute O(3) damage was not uniform within the crowns of aspen suckers; it was most severe in the mature, fully expanded photosynthesizing leaves. Young expanding leaves showed no visible signs of acute O(3) damage contrary to expectations. Stomatal conductance played a primary role in the severity of acute O(3) damage as it directly controlled O(3) uptake. Maple sprouts, which had lower stomatal conductance, smaller stomatal aperture, higher stomatal density and larger leaf surface area, were tolerant of acute O(3) exposure. Moreover, elevated CO(2) did not ameliorate the adverse effects of acute O(3) dose on aspen and maple sprouts, in contrast to its ability to counteract the effects of long-term chronic exposure to lower O(3) levels.     

Ecological restoration of mined-out areas of dry tropical environment, India

The objective of the present study was to evince the long-term changes after natural revegetation and experimental revegetation of the coal mine spoils with respect to total plant biomass, available plant nutrients, nitrogen transformation and microbial biomass N (MBN) in dry tropical environment of India. Total plant biomass (above- and below-ground), plant available nitrogen, soil nitrogen mineralization and microbial biomass N (MBN) were studied for 2 years in 5 and 10 years old naturally vegetated and revegetated coal mine spoils, and dry tropical forest ecosystem of India. In forest ecosystem, the above ground biomass values ranged from 3,520 to 3,630 kg ha(-1) and belowground from 6,280 to 6,560 kg ha(-1). Plant available nitrogen ranged from 16.76 to 23.21 microg g(-1), net N-mineralization from 9.8 to 48.53 microg g(-1) month(-1) and MBN from 26.4 to 80.02 microg g(-1). In naturally revegetated mine spoil, the above ground biomass values ranged from 1,036 to 1,380 kg ha(-1) and belowground from 2,538 to 3,380 kg ha(-1). Plant available nitrogen ranged from 7.33-17.14 microg g(-1), net N-mineralization from 3.1 to 12.46 microg g(-1) month(-1) and MBN from 14.2 to 35.44 microg g(-1). In revegetated mine spoil, the above ground biomass values ranged from 1,224 to 1,678 kg ha(-1) and belowground from 2,870 to 4,130 kg ha(-1). Plant available nitrogen ranged from 9.4 to 18.83 microg g(-1), net N-mineralization from 4.2 to 16.2 microg g(-1) month(-1) and MBN from 21.6 to 42.6 microg g(-1). The mean plant biomass values in 5 and 10 years mine spoils was lower compared to forest ecosystem by 2.5 and 2 times, respectively. N-mineralization value in 5 year mine spoil was 3.5 times lower and in 10 years mine spoil 2 times lower compared to forest ecosystem. The MBN value was about 2 times lower in both 5 and 10 year mine spoils compared to native forest. MBN was positively related to the re-vegetation age of the mine spoil.     

Critical analysis of the potential of Ipomoea nil'Scarlet O'Hara' for ozone biomonitoring in the sub-tropics

This study aimed to analyze critically the potential of Ipomoea nil'Scarlet O'Hara' for O(3) biomonitoring in the sub-tropics. Four field experiments (one in each season of 2006) were carried out in a location of the city of S?o Paulo mainly polluted by O(3). Each experiment started with 50 plants, and lasted 28 days. Sub-lots of five plants were taken at intervals between three or four days long. Groups of four plants were also exposed in closed chambers to filtered air or to 40, 50 or 80 ppb of O(3) for three consecutive hours a day for six days. The percentage of leaf injury (interveinal chloroses and necroses), the concentrations of ascorbic acid (AA) and the activity of superoxide dismutase (SOD) and peroxidases (POD) were determined in the 5th, 6th and 7th oldest leaves on the main stem of the plants taken in all experiments. Visible injury occurred in the plants from all experiments. Seasonality in the antioxidant responses observed in plants grown under field conditions was associated with meteorological variables and ozone concentrations five days before leaf analyses. The highest levels of antioxidants occurred during the spring. The percentage of leaf injury was explained (R(2) = 0.97, p < 0.01) by the reduction in the levels of AA and activity of POD five days before the leaf analyses and by the reduction in the levels of particulate matter, and enhancement of temperature and global radiation 10 days before this same day. Although I. nil may be employed for qualitative O(3) biomonitoring, its efficiency for quantitative biomonitoring in the sub-tropics may be compromised, depending on how intense the oxidative power of the environment is.     

Contemporary estimates of atmospheric nitrogen deposition to the watersheds of New York State, USA

Atmospheric inputs of reactive nitrogen (N) to ecosystems are a particular concern in the northeastern USA, including New York State, where rates of atmospheric N deposition are among the highest in the nation. We calculate the seasonal and annual spatial variations of contemporary inorganic atmospheric N deposition loading to multi-scale watersheds across New York State using numerous monitoring datasets of precipitation and ambient atmospheric N concentrations. Our models build upon and refine previous efforts estimating the spatial distribution of N deposition. Estimates of total inorganic wet deposition (NH4-N + NO3-N) across New York ranged from 4.7 to 10.5 kg ha(-1) yr(-1) under contemporary conditions (averaged 2002-2004), and both seasonal and annual predicted rates of inorganic N deposition (NH4-N, NO3-N, and total) fit relatively well with that of observed measurements. Our results suggest that "hot spots" of N deposition are, for the most part, spatially distributed according to geographic positions (i.e., relative location from sources and the Great Lakes system) and elevation. We also detect seasonal variations in deposition, showing that total wet atmospheric inorganic N deposition inputs to watersheds (extracted from the four-digit HUC calculations) are highest during the spring (mean = 2.4 kg ha(-1), stddev = 0.29) and lowest during the winter months (mean = 1.4 kg ha(-1), stddev = 0.23). Results also suggest that wet NO3(-) consistently comprises a slightly higher proportion of wet N deposition than wet NH4+ throughout watersheds of New York, ranging from 2.5 to 6.1 kg NO3-N ha(-1) yr(-1) compared to NH4+, which ranges from 2.2 to 4.4 kg NH4-N ha(-1) yr(-1).     

Evaluation of air pollution phytotoxicity in a seasonally dry tropical urban environment

This study was conducted in the urban environment of Varanasi, India, to evaluate the plant responses to urban air pollution. Twenty sites were selected in four different zones of the city. At each site, seven woody perennials of same age classes were selected. Out of the four zones (I, II, III and IV), zone IV was used as a reference (control) zone as it received the minimum pollution input. Plant species growing in polluted and control areas were compared with respect to foliar dust load, per cent leaf area injury, leaf area, specific leaf weight and chlorophyll, ascorbic acid, SO ₄ ^2– S and total N concentration in the leaves. Results indicated that the air pollution level in Varanasi causes leaf damage, reduces leaf area, specific leaf weight and chlorophyll, ascorbic acid and total N concentrations in the leaves. Sulphur concentration in leaves increased with increasing level of SO₂ in the ambient air. The magnitude of such changes was maximum at the zone receiving maximum pollution load. Carissa carandas was found to be the most sensitive species and Bougainvillea spectabilis, the least. The study shows that the urban air pollution level in Varanasi is detrimental for the growth of plants involved in this study.     

Temporal and spatial variations of nutrients in the Ten Mile Creek of South Florida, USA and effects on phytoplankton biomass

Water quality throughout south Florida has been a major concern for many years. Nutrient enrichment in the Indian River Lagoon (IRL) is a major surface water issue and is suggested as a possible cause of symptoms of ecological degradation. In 2005-06, water samples were collected weekly from seven sites along Ten Mile Creek (TMC), which drains into the Indian River Lagoon, to investigate and analyze spatial and temporal fluctuations of nutrients nitrogen (N) and phosphorus (P). The objective of this study was to understand the relationships among chlorophyll a concentration, nutrient enrichment and hydrological parameters in the surface water body.High median concentrations of total P (TP, 0.272 mg L(-1)), PO4-P (0.122 mg L(-1)), and dissolved total P (DTP, 0.179 mg L(-1)); and total N (TN, 0.988 mg L(-1)), NO3(-)-N (0.104 mg L(-1)), NH4+-N (0.103 mg L(-1)), and total Kjeldahl N (TKN, 0.829 mg L(-1)), were measured in TMC. The concentrations of TP, PO4-P, DTP, TN, NO3(-)-N, NH4+-N, and TKN were higher in summer and fall than in winter and spring. However, chlorophyll a and pheophytin concentrations during this period in TMC varied in the range of 0.000-60.7 and 0.000-17.4 microg L(-1), with their median values of 3.54 and 3.02 microg L(-1), respectively. The greatest mean chlorophyll a (10.3 microg L(-1)) and pheophytin (5.71 microg L(-1)) concentrations occurred in spring, while the lowest chlorophyll a (1.49 microg L(-1)) and pheophytin (1.97 mug L(-1)) in fall. High concentrations of PO4-P (>0.16 mg L(-1)), DTP (>0.24 mg L(-1)), NO3(-)-N (>0.15 mg L(-1)), NH4+-N (>0.12 mg L(-1)), and TKN (>0.96 mg L(-1)), occurred in the upstream of TMC, while high concentrations of chlorophyll a (>6.8 mug L(-l)) and pheophytin (>3.9 microg L(-l)) were detected in the downstream of TMC. The highest chlorophyll a (11.8 mug L(-l)) and pheophytin (6.06 microg L(-l)) concentrations, however, were associated with static and open water conditions. Hydrological parameters (total dissolved solid, electrical conductivity, salinity, pH, and water temperature) were positively correlated with chlorophyll a and pheophytin concentrations (P < 0.01) and these factors overshadowed the relationships between N and P concentrations and chlorophyll a under field conditions. Principal component analysis and the ratios of DIN/DP and TN/TP in the water suggest that N is the limiting nutrient factor for phytoplankton growth in the TMC and elevated N relative to P is beneficial to the growth of phytoplankton, which is supported by laboratory culture experiments under controlled conditions.     

Biogeochemistry of the Penobscot River watershed, Maine, USA: nutrient export patterns for carbon, nitrogen, and phosphorus

Watershed exports of carbon, nitrogen, phosphorus, major solutes, and suspended sediments were examined during five water years in the Penobscot River basin, which forms part of the Gulf of Maine watershed. Mean annual exports of dissolved organic carbon (DOC) in the Penobscot River were 58 kg C ha(-1) year(-1), whereas cumulative yearly watershed flux of DOC during the study period ranged from 8.6 to 16.1 × 10(10) g C year(-1) and averaged 11.7 × 10(10) g C year(-1). Watershed exports of total soluble N (TN) and total soluble P in the Penobscot River averaged 1.9 and 0.02 kg ha(-1) year(-1), respectively. Companion studies in two other major Maine rivers indicated that mean annual exports of DOC and TN in the Androscoggin River were 40 kg C ha(-1) year(-1) and 2.0 kg N ha(-1) year(-1), whereas exports in the Kennebec River were 43 kg C ha(-1) year(-1) and 2.2 kg N ha(-1) year(-1). Extrapolation of results from this investigation and a previous complementary study indicates that estuaries and coastal waters in the Gulf of Maine receive at least 1.0 × 10(10) g N year(-1) and 2.5 × 10(11) g C year(-1) in combined runoff from the four largest Maine river basins. Soluble exports of Ca + Mg + Na minus wet deposition inputs of cations in the Penobscot system were approximately 1,840 mol(c) ha(-1) year(-1), which represents a minimum estimate of cation denudation from the watershed. Based on its low N and P export rates, the Penobscot River watershed represents an example of reference conditions for use as a benchmark in ecological assessments of river water quality restoration or impairment. In addition, the biogeochemical metrics from this study provide an historical baseline for analysis of future trends in nutrient exports from the Penobscot watershed as a function of changing climatic and land use patterns.     

Assessment of six Indian cultivars of mung bean against ozone by using foliar injury index and changes in carbon assimilation, gas exchange, chlorophyll fluorescence and photosynthetic pigments

Six Indian cultivars of Vigna radiata L. (HUM-1, HUM-2, HUM-6, HUM-23, HUM-24 and HUM-26) were exposed with ambient and elevated (ambient + 10 ppb ozone (O₃) for 6 h?day^?1) level of O₃ in open top chambers. Ozone sensitivity was assessed by recording the magnitude of foliar visible injury and changes in various physiological parameters. All the six cultivars showed visible foliar symptoms due to O₃, ranging 7.4 to 55.7 % injured leaf area. O₃ significantly depressed total chlorophyll, photosynthetic rate (Ps), quantum yield (F _v/F _m) and total biomass although the extent of variation was cultivar specific. Cultivar HUM-1 showed maximum reduction in Ps and stomatal conductance. The fluorescence parameters also indicated maximum damage to PSII reaction centres of HUM-1. Injury percentage, chlorophyll loss, Ps, F _v/F _m and total biomass reduced least in HUM-23 depicting highest O₃ resistance (R%).     

Antioxidant responses in the leaves of mercury-treated Eichhornia crassipes (Mart.) Solms.

Eichhornia crassipes (Mart.) Solms. plantlets were grown in 0.1 and 1.0 ppm treatment solutions of Hoagland’s hydroponic solutions modified with Hg(NO₃)₂ in order to examine the specific cellular and biochemical mechanisms involved in the tolerance of this plant exposed to mercury. This study assessed the responses of chloroplast pigments, i.e., carotenoids and chlorophylls, and evaluated the enzymatic and nonenzymatic antioxidant systems. Inductively coupled plasma-atomic emission spectrometry (ICP-AES) revealed varying Hg²⁺ levels in the young and mature leaf tissues, with greater amounts of Hg²⁺ found in the tissues of the young leaves. Total chlorophyll levels, notably those of chlorophyll a, chlorophyll b, and carotenoids, showed significant elevation in young leaf tissues, while a decrease in their levels was observed in mature leaf tissues in comparison to those of the control plants. These results lend support to the protective role of increased chlorophyll and carotenoid levels in the photosynthetic apparatus of young E. crassipes leaves in the presence of Hg²⁺. The antioxidant responses of Hg-treated E. crassipes plants were also measured, revealing a highly significant increase in catalase units, catalase and ascorbate peroxidase activities, and mercury-binding thiols in leaves from Hg-treated plants. Moreover, substantial differences in the degree of oxidative injury between the cells in leaves from the control and Hg-treated plants were evidenced by the lipid peroxidation activities monitored. The Hg-treatment-induced significant decrease in malondialdehyde (MDA) levels was observed in 0.1-ppm Hg(NO₃)₂-exposed plants, while a highly significant increase in MDA levels was noted in 1.0-ppm Hg(NO₃)₂-exposed plants. The high degree of lipid peroxidation at 1.0-ppm Hg treatment was evidently counteracted by the compensatory protective mechanism brought about by the increased levels in chloroplast pigments and the enhanced activities of the antioxidant systems. E. crassipes responded to mercury treatments by enhancing the synthesis of chlorophyll and carotenoid pigments, enzymatic, and nonenzymatic antioxidant substances, concomitantly increasing the antioxidative activities, thus rendering E. crassipes capable of tolerating Hg-induced stress. The potential of E. crassipes as a phytoremediator is evident.     

Biological responses of workplace particles and their association with adverse health effects on miners

Epidemiological research has demonstrated the relationship between exposure to quartz dust and an elevated risk of pneumoconiosis and possible elevated risk of cancer. The current study was designed to evaluate the biological responses of workplace particles containing crystalline silica using an in vitro cell test. Respirable particle samples were sampled from four tin mines, where the standardized mortality ratio (SMR) for pneumoconiosis was 51.6 and SMR for lung cancer was 2.2 in dust-exposed miners. Alveolar macrophages (AM) are considered as the target cells for primary dust effects. The samples were then measured at 15, 30, 60 and 120 microg particle per 10(6) AM for cytoxicity with the release of glucuronidase, lactate dehydrogenase, for reactive oxygen damage with H(2)O(2) release, and for ability to induce fibrosis using the secretion of tumor necrosis factor-alpha (TNF-alpha). Pure quartz (DQ12) and corundum were used as controls. The results showed the samples from tin mines caused a higher cytoxicity when compared to corundum, yet lower when compared to quartz. However, reactive oxygen species release (148-177 nmol/3 x 10(5) AM in high concentration of 120 microg/10(6) AM) induced by the samples were significantly higher than that induced by quartz (57 nmol/3 x 10(5) AM) and corundum (62 nmol/3 x 10(5) AM). Furthermore, particle samples induced higher TNF-alpha secretion than corundum, the samples from Limu tin mine induced much higher TNF-alpha levels than that induced by DQ12 quartz. The results from the in vitro tests help elucidate the degree of hazard of dust particles in tin mines. The in vitro reaction patterns of AM also constitute a powerful tool to monitor biological and pathogenic responses of humans following dust particle exposure.     

Effects of treated sewage sludge levels on temporal variations of some soil properties of a Typic Xerofluvent soil in Menemen Plain, Western Anatolia, Turkey

The aim of this study is to determine effects of treated sewage sludge (TSS) levels as an organic matter (OM) resource on temporal variations of some soil properties of a Typic Xerofluvent soil. The experiment was conducted in Menemen Plain, in the Western Anatolia Region of Turkey (latitudes 38 degrees 34'48.22'-38 degrees 34'49.24' N; longitudes 27 degrees 1'23.05-27 degrees 1'24.14' E) in the years of 2003 and 2004. Moist TSS was added to the soil at the rates of 0, 30, 60 and 90 t ha(-1) on May 1, 2003. Peanut (Arachis hypogaea) was planted as first crop. On the other hand, mixture of green barley (Hordeum vulgare) and common vetch (Vicia sativa L.) was planted as second crop. During the experiment, soil samples were taken in five different periods (1st, June 18, 2003; 2nd, November 13, 2003; 3rd, April 30, 2004; 4th, October 10, 2004 and 5th, May 12, 2004). The results showed that increasing TSS application to Typic Xerofluvent soil was significantly increased total salt, OM, total porosity, micro porosity, macro porosity, field capacity, wilting point, available water content, structure stability index and aggregation percentage values of soil when compared with control. Meanwhile, particle density, dry bulk density and nonaggregated silt + clay values of soil decreased. On the other hand, soil reaction (pH), lime content and total silt + clay values of soil did not significantly change. In the course of time, depending on decomposing of TSS organic materials in soil, effect of TSS levels on soil properties decreased particularly in the last periods. For this reason, it can be recommended that 90 t ha(-1) moist TSS can be added once in 2 years for improving soil properties of Typic Xerofluvent soil, which are characterized by low OM content.     

Atmospheric NO2 and NH3 deposition into a typical agro-ecosystem in Southeast China

The dry deposition of atmospheric nitrogen (including NO(2) and NH(3)) into a typical agro-ecosystem in Southeast China during 2006-2007 was estimated. Results indicated that the dry deposition velocities of NO(2) and NH(3) ranged from 0.04-0.24 cm s(-1) and 0.09-0.47 cm s(-1), respectively. The higher values appeared in the non-crop growing period. Concentrations of atmospheric NO(2) and NH(3) ranged from 24.64-104.10 μgN m(-3) and 14.40-389.6 μgN m(-3), respectively. Variation of the NH(3) mixing ratio showed a clear double-peak. NO(2) and NH(3) deposition fluxes were 74.68-80.75 kgN ha(-1), which was equivalent to 162.4 and 175.5 kg ha(-1) of urea applied in 2006-2007. The N deposition fluxes were 13.91-40.38 and 5.33-22.73 kgN ha(-1) in peanut and rice growing periods, accounting for 8.18%-40.38% and 2.13%-23.06% of N fertilizer usages, respectively. NO(2) and NH(3) deposition were significant for the red soil farmland.     

Developmental strategies for sustainable ecosystem on mine spoil dumps: a case of study

An important goal of ecological rehabilitation is to accelerate natural successional processes to increase biological productivity, soil fertility and biotic control over biogeochemical fluxes within the recovering ecosystems. A new approach called Microbe Assisted Green Technology (MAGT) is an integrated biotechnological approach developed at National Environmental Engineering Research Institute (NEERI) through exhaustive laboratory as well as field studies and serve as a model for land reclamation and development of lush green vegetation on mine overburdens. One year old seedlings of native tree species were planted on 6.3 ha area of manganese mine overburden at Gumgaon under Manganese Ore India Ltd., Maharashtra, India. Continuous efforts resulted in nutrient rich soil with high N, P, K and organic carbon; well developed biodiversity, including bacteria, fungi, higher plants (more than 350 species) and different classes of animals. Planted trees accumulated 698 t ha( - 1) above ground biomass and 143 t ha( - 1) below ground mass. This was achieved in 18 years by MAGT, which otherwise takes hundreds of years.     

Persistence of metsulfuron-methyl in wheat crop and soil

Possible bioaccumulation of pesticides in crop produce may cause ailing effect on animal and human. Thus there is a need to evaluate these chemicals in the soil and crop produce at harvest. Metsulfuron-methyl is a post-emergence herbicide. It is highly active to control broad-leaf weeds in cererals, pasture and plantation crops. Metsulfuron-methyl was applied at 3, 4, 5, and 8 g a.i. ha(-1) rates, after 30 days of sowing in wheat as post-emergence herbicide. Soil samples treated with metsulfuron-methyl were collected after 30 and 60 days along with control and at harvest after herbicide application and analyzed for residues by High Performance Liquid Chromatography (HPLC) using photo diode array detector at 220 nm. Wheat grains and straw samples were sampled at harvest. At harvest the residue level of metsulfuron-methyl in soil was found below the detection limit at 3-5 g a.i. ha(-1) application rates and 0.002 microg g(-1) at 8 g a.i. ha(-1), respectively. No residues of metsulfuron-methyl were detected in wheat grains at 3-4 g a.i. ha(-1) rates. However 0.002 microg g(-1) residues were detected in wheat straw at 5 and 8 g a.i. ha(-1) application rates. It can be concluded that metsulfuron-methyl application at 3-4 g a.i. ha(-1) can be safely applied to the wheat crop as post-emergence herbicide.     

Assessment of soil nitrogen and related enzymes as influenced by the incorporation time of field pea cultivated as a catch crop in Alfisol

The effect of the time of catch crop (field pea) incorporation [catch crop incorporated in the autumn (A) or in the spring (B) versus plots without a catch crop (C)] on the soil enzymes related to N transformation (urease – UR, protease – PRO, nitrate reductase – NR, arginine ammonification rate – AAR), the total N and mineral N as well as microbial biomass N (MBN) contents were investigated in a 3-year experiment. The catch crop was sown at the beginning of August and plowed in the autumn in 2008, 2009 and 2010 or left as mulch during the winter. Soil samples for microbial activity were taken from spring barley plots that were grown in 2009, 2010 and 2011 before sowing (March), during the tillering phase (May), shooting (June) and after the harvesting of spring barley (August). The use of catch crop significantly increased the soil mineral and MBN contents as well as the activities of PRO and NR as compared to the control soil. The spring incorporation of the field pea significantly increased the MBN content in contrast to the autumn application, while the activity of N-cycle enzymes were clearly unaffected (UR and AAR) regardless of the time of the incorporation of field pea or else the results were inconsistent (PRO and NR). When the catch crop was incorporated in the spring, a significantly higher content of mineral N as compared to autumn incorporation was noted on only two of the four sampling dates. The enzymatic activity (PRO and AAR) was about 1.3-2.8 times higher in May and June as compared with March and August. Both spring or autumn incorporation of catch crop can be a useful management practice to increase the soil mineral N content and enhance the soil biological activity.     

垃圾渗滤液氨态氮与挥发性脂肪酸馏出规律研究

为了提高垃圾渗滤液氨态氮（NH4^＋-N）与挥发性脂肪酸（VFA）联合滴定分析结果的准确性,试验研究了NH4^＋-N与VFA的馏出规律,并在此基础上对联合滴定方法的优化进行了探讨。结果表明：测得的NH4^＋-N质量浓度（Y1）、VFA浓度（Y2）与馏出液体积（X）分别符合函数关系式;联合滴定分析过程中,渗滤液样品NH4^＋-N质量浓度应稀释至150mg／L-200mg／L,NH4^＋-N馏出液应大于蒸馏液体积的60％;VFA分析应采用“一次蒸馏80％＋二次蒸馏”的方式,且VFA二次馏出液应大于蒸馏液体积的75％（相对误差限εr〈5％）。     

Determination of imazosulfuron persistence in rice crop and soil

Imazosulfuron is a new post-emergence sulfonylurea herbicide. It is highly active at low application rates to control annual and perennial broad-leaf weeds and sedges in rice. There is increasing concerned about the persistence of pesticide residues in soils, crop produce and subsequent contamination of groundwater. Thus persistence of imazosulfuron residues under field condition was evaluated. Imazosulfuron was applied at 30, 40, 50 and 60 a. i. g ha ⁻¹ rates, 4 days after transplanting of rice as post-emergence herbicide. Soil and plant samples treated with imazosulfuron were collected at 60, 90 and 120 days after herbicide application and analyzed for residues. Rice grains and straw samples were sampled at harvest (120 days). Residues of imazosulfuron in soil were not found after 90 and 120 DAS (days after spraying). Rice grains contained 0.006, 0.009 μg g⁻¹ residues at 50 and 60 g ha ⁻¹ application rates. 0.009 and 0.039 μg g⁻¹ residues of imazosulfuron were detected at 50 and 60 g/ha rates respectively in rice straw. Residues of imazosulfuron were not detected applied at 30 and 40 g ha⁻¹ in rice grains and straw, respectively and can be safely applied to the transplanted rice.     

Insights into ozone deposition patterns from decade-long ozone flux measurements over a mixed temperate forest

Long-term fluxes of ozone (O(3)) were measured over a mixed temperate forest using the aerodynamic gradient method. The long-term average O(3) flux (F) was -366 ng m(-2) s(-1) for the period 2000-2010, corresponding to an average O(3) concentration of 48 μg m(-3) and a deposition velocity v(d) of 9 mm s(-1). Average nocturnal ozone deposition amounted to -190 ng m(-2) s(-1), which was about one third of the daytime flux. Also during the winter period substantial O(3) deposition was measured. In addition, total O(3) fluxes were found to differ significantly among canopy wetness categories. During the day, highest deposition fluxes were generally measured for a dry canopy, whereas a rain-wetted canopy constituted the best sink at night. Flux partitioning calculations revealed that the stomatal flux (F(s)) contributed 20% to the total F but the F(s)/F fraction was subject to seasonal and diurnal changes. The annual concentration-based index AOT40 (accumulated dose over a threshold of 40 ppb) and the Phytotoxic Ozone Dose (POD(1) or accumulated stomatal flux above a threshold of 1 nmol m(-2) s(-1)) were related in a curvilinear way. The O(3) deposition was found to be largely controlled by non-stomatal sinks, whose strength was enhanced by high friction velocities (u(*)), optimizing the mechanical mixing of O(3) into the canopy and the trunk space. The long-term geometrical mean of the non-stomatal resistance (R(ns)) was 136 s m(-1) but lower R(ns) values were encountered during the winter half-year due to higher u(*). The R(ns) was also subject to a marked diurnal variability, with low R(ns) in the morning hours, when turbulence took off. We speculate that non-stomatal deposition was largely driven by scavenging of ozone by biogenic volatile organic compounds (BVOCs) and especially NO emitted from the crown or the forest floor.     

Nitrous oxide fluxes from the littoral zone of a lake on the Qinghai-Tibetan Plateau

Nitrous oxide (N(2)O) fluxes were measured in six littoral mirco-zones of Lake Huahu on Qinghai-Tibetan Plateau in the peak growing season of years of 2006 and 2007. The weighted mean N(2)O flux rate was 0.08 mg N m(-2) h(-1) (ranged from -0.07 to 0.35 mg N m(-2) h(-1)). The result was relatively high in the scope of N(2)O fluxes from boreal and temperate lakes. Emergent plant zones (Hippuris vulgaris and Glyceria maxima stands) recorded the highest N(2)O flux rate (0.11 ± 0.24 and 0.08 ± 0.17 mg N m(-2) h(-1), respectively). Non-vegetated lakeshore recorded the lowest N(2)O flux (0.03 ± 0.11 mg N m(-2) h(-1)), lower than that from the floating mat zone of Carex muliensis (0.05 ± 0.18 mg N m(-2) h(-1)), the floating-leaved plant zone of Polygonum amphibium (0.07 ± 0.11 mg N m(-2) h(-1)), and the wet meadow (0.07 ± 0.15 mg N m(-2) h(-1)). Standing water depths were important factors to explain such spatial variations in N(2)O fluxes. Significant temporal variations in N(2)O fluxes were also found. Such temporal variation in N(2)O flux in the littoral zone may be dependent on the interaction of water regime and thermal conditions, instead of the latter solely. These results showed the importance of the littoral zone of lake, especially the emergent plant zone, as a hotspot of N(2)O fluxes in such grazing meadows.     

Determination of CH4, CO2 and N2O in air samples and soil atmosphere by gas chromatography mass spectrometry, GC-MS

A method for determination of the climate gases CH4, CO2 and N2O in air samples and soil atmosphere was developed using GC-MS. The method uses straightforward gas chromatography (separation of the gases) with a mass spectrometric detector in single ion mode (specific determination). The gases were determined with high sensitivity and high sample throughput (18 samples h(-1)). The LOD (3sigma) for the gases were 0.10 micro L L(-1) for CH4, 20 microL L(-1) for CO2 and 0.02 microL L(-1) for N2O. The linear range (R2 = 0.999) was up to 500 microL L(-1) for CH4, 4000 microL L(-1) for CO2 and 80 microL L(-1) for N2O. The samples were collected in 10 mL vials and a 5 microL aliquot was injected on column. The method was tested against certified gas references, the analytical data gave an accuracy within +/-5% and a precision of +/-3%. The presence of < or = 10% by volume of C2H2 (often used experimentally to prevent N2 formation from N2O) did not interfere with detection for the targeted trace gases.