Age-related settlement success by cyprids of the barnacleBalanus amphitrite,with special reference to consumption of cyprid storage protein

Newly molted (0-d-old) cyprids of the barnacleBalanus amphitrite Darwin were prevented from settling for 0 to 14 d at four different temperatures (25, 20, 15 and 5°C treatments). The effect on settlement success of prolonging the cyprid lifetime was evaluated using a nitrilocellulose membrane assay. In addition, protein extract prepared from these cyprids was analyzed using gel electrophoresis to characterize the effect of age on protein content and composition. Settlement success was significantly affected for larvae aged at 25 (P < 0.001), 20 (P < 0.001) and 15°C (P < 0.05), while differences in settlement success between age groups was negligible at 5°C (P = 0.09). Settlement success of cyprids increased with time for up to 3 d (P < 0.001, Phase 1), following which settlement success significantly declined (P < 0.001, Phase 11). Temperature had no significant effect on settlement in Phase I (P = 0.17), but did enhance the decline in settlement success with age during Phase II (P < 0.001). Gel electrophoresis revealed a significant decline in the quantity of the cyprid storage protein CMP (Cyprid Major Protein) with increasing age at 25, 20 and 15°C, but CMP levels remained constant at 5°C. These results suggest that, upon molting to the cyprid stage, larvae may still require a settlement-competence attainment period. This may be achieved by CMP utilization during Phase I, depletion of which during Phase II may be responsible for reduction in settlement success with cyprid age such that remaining CMP stores can no longer support the production of adult structures following settlement.     

Initial contact,exploration and attachment of barnacle (Balanus amphitrite) cyprids settling in flow

Settlement responses of barnacle (Balanus amphitrite) cyprids to boundary-layer flows were examined in laboratory flume-experiments. The leading-edge configuration of flat plates was altered in order to manipulate flows without changing surface topography or freestream velocity. Settlement along the plates correlated strongly with downstream gradients in shear stress. Analyses of video images taken during the experiments indicate that cyprids first contact plates in regions where plate-ward advection is high, and subsequent exploratory movement along the plate is oriented with flow direction at the plate surface. After exploration, cyprids reject a surface more frequently in a fast flow (10 cm s^–1 freestream velocity) than in a slow flow (5 cm s^–1), but rejection occurs in shear stresses well below the threshold that would prevent attachment and exploration. A higher rejection rate does not result in lower settlement, however, since contact rate is higher in fast than slow flows. The movement of cyprids in flow thus appears to be a passive transport process during the initial contact stage of settlement, but an active behavioral response to flow direction and shear stress during later stages of exploration and attachment.     

Influence of food concentration,temperature and salinity on the larval development ofBalanus amphitrite

Influence of food concentration (0.5, 1 and 2 x 10⁵ cell ml^–1 ofSkeletonema costatum), temperature (20 and 30°C) and salinity (15, 25 and 35) on the larval development ofBalanus amphitrite (Cirripedia: Thoracica) was examined. The mortality rate at 20°C was lower than at 30°C in general. Increase in food concentration from 0.5 to 1 x 105 cells ml^–1 improved the survival rate, but this was not evident when food concentration was increased to 2 x 105 cells ml^–1. The results indicate that food availability and temperature jointly determine the energy allocation for metamorphic progress. It was observed that the influence of the tested variables varied with instar. At 20 °C the mean duration of the second instar exceeded 3 d and was much longer than other instar durations. The fourth, fifth and sixth instars and the total naupliar period showed that the effect of different salinities at given food concentrations was negligible at 20°C, while at 30°C there was a marked decrease in duration with increasing salinity.     

沙尘暴对暴露儿童健康效应的研究

为了研究沙尘暴对儿童可能造成的急性危害,以沙尘暴源区——甘肃省武威市的某2所小学3～5年级的1040名学生(年龄8～14岁)为研究对象,在2004年和2005年的3～5月沙尘暴频发期间进行健康状况问卷调查,共调查4场沙尘暴事件,所调查的症状有咳嗽、咯痰、气短、肺部喘鸣、胸部憋闷、咽干口苦、眼睛干涩、流泪、流涕、打喷嚏、心情压抑等11种常见的症状.结果表明,沙尘暴发生当天(滞后0d)儿童各种所查症状发生的相对危险度(RR)均有不同程度地升高,沙尘暴过后(滞后1～5d),儿童各种症状的RR逐渐降低,沙尘暴过后4d(滞后4d),几乎所有症状均恢复正常.暴露儿童在沙尘暴发生当天,除肺部喘鸣和胸部憋闷发生的RR升高不显著外(p>0.05),其他所查症状发生的RR均有显著性升高(p<0.05,p<0.01,p<0.001).沙尘暴过后虽然各种症状RR均有所下降,但下降速度不同:在滞后1～2d,气短、眼睛干涩、流泪和心情压抑等4种症状RR仍显著高于沙尘暴前,直到滞后3d才恢复到正常水平;而咳嗽、咯痰、咽干口苦和打喷嚏等4种症状RR在滞后4d才恢复正常;流涕症状RR在滞后5d才基本恢复正常.不同年度和不同时期的沙尘暴事件对儿童健康症状的影响有所不同.从以上结果可以看出:沙尘暴对儿童的健康既有当日急性效应又有一定的短期滞后效应.     

金钟藤的年轮生长量与气候因子的相互关系

分析了金钟藤(Merremiaboisiana(Gagn.)vanOoststr.)的年轮特征,并探讨了它与温度等气候因子的关系。金钟藤的年轮极不规则,有许多自然隆起和裂缝。金钟藤的年轮生长量通过格子法来估算。结果表明金钟藤年轮的年平均生长量与温度呈极显著正相关,与降水、日照时数和相对湿度相关性不显著。罗浮山地区降水和日照时数基本能够满足金钟藤的生长,结合相关研究结果推测:温度可能是金钟藤快速生长的主要驱动因子。     

中国饮水型砷中毒区的水化学环境与砷中毒关系

中国各饮水型砷中毒区的水化学特点受沉积环境和气候因素所控制,砷中毒的流行和发病程度与其地下水的水化学环境、水中砷的形态和价态有密切联系。在实地考察监测和对台湾、新疆、内蒙古、山西、吉林饮水型砷中毒病区环境和地下水水化学特征总结的基础上,系统分析了饮水水源中总As、As(III)、甲基胂、腐植酸与砷中毒的关系,揭示了不同病区病情差异的原因。研究表明,除台湾外,各砷中毒区均分布在干旱半干旱区;各病区多分布在沉积盆地中心或平原内相对低洼的地带,饮用的地下水均取自中新生代地层;砷中毒病情不仅与总砷含量有明显的剂量-效应关系,还与As(III)和甲基胂的浓度直接相关。台湾、内蒙古和山西病区地下水为富含有机质的复杂还原环境,水中不仅As(III)含量高,且检出有机物、腐植酸和甲基胂,新疆和吉林病区地下水为以无机砷中As(V)为主的氧化环境,吉林病区未检测出甲基胂,这是新疆与吉林病区患病率较低的主要原因。研究成果可为区域防病改水、砷中毒的预报提供重要技术支撑。     

Relationship between photosynthesis and non-photochemical quenching of chlorophyll fluorescence in two red algae with different carotenoid compositions

Algae are continuously exposed to short-term fluctuations in irradiance. We investigate how two red algae species regulate photosynthetic efficiency to cope with such changes and identify some strategies that differ from higher plants. Two red algae, Gracilaria domingensis and Kappaphycus alvarezii, with antheraxanthin and lutein as major xanthophylls, respectively, reacted to the onset of low light (below E _k) with a substantial decrease of NPQ. This is different from higher plants, but similar to previous observations in, e.g. cyanobacteria where it indicates an increase in the effective absorbance cross-section of Photosystem II (PSII) by state transition. Kinetic studies in continuous light revealed a high susceptibility of PSII to light stress ((1-q _P)/NPQ) in K. alvarezii immediately after the sudden onset of high light, followed by a decrease. This was caused by a slower onset of NPQ in K. alvarezii, followed by acclimation. In G. domingensis, susceptibility of PSII to light stress was stable with time, but absolute values of (1-q _P)/NPQ were higher than in K. alvarezii. These observations suggest that K. alvarezii may be better adapted to high light levels, but is less well prepared for large sudden changes in irradiation. In K. alvarezii, photosynthesis continued to increase with increasing irradiation when NPQ was saturated. As (1-q _P) and NPQ were still balanced in this situation, most likely, processes other than photosynthetic oxygen release are responsible for the increasing net O₂ production observed.     

Relationship between Coefficient of Inbreeding and Parasite Burden in Endangered Gazelles

Abstract: We studied the effects of inbreeding depression on parasite infection in three species of endangered gazelles: Gazella cuvieri , G. dama, and G. dorcas . Coefficients of inbreeding were calculated for all individuals because complete genealogies were available. The levels of inbreeding differ both intra- and interspecifically. We collected samples of feces and determined nematode infection by counting nematode eggs in the samples. At the interspecific level, the species with the highest mean levels of inbreeding (  G. cuvieri ) had the highest levels of gastrointestinal parasites. Analyses done at the intraspecific level revealed a positive relationship between individual coefficient of inbreeding and parasite infection in G. cuvieri , but not in the species with the intermediate and lowest levels of inbreeding. Our findings suggest that high levels of inbreeding may make individuals more susceptible to parasitism, even under favorable environmental conditions, so this factor should be taken into account by those managing endangered species.     

不同施肥方式下紫色水稻土土壤肥力变化规律研究

通过连续 12 a田间小区定位试验 ,研究了在不同施肥方式下 ,连年稻 -麦轮作的滇中地区紫色冲积性潴育型水稻土土壤肥力及产量变化的规律。结果表明 :厩肥 (O)处理与厩肥配合氮磷处理 (ONP)12 a后土壤肥力明显高于对照和单施化肥的 NP、NK、PK、NPK等 4处理 ,土壤有机质含量增加 8.4 g/kg以上 ,速效氮、速效磷、速效钾含量增加 14.6 %以上 ,土壤容重下降 0 .16以上 ;单施化肥的处理 ,土壤肥力的变化与对照无显著差异。     

大学生健康与头发中铅含量的关系

通过实验、调查、查资料的方式，对“大学生发铅与人体健康”这一课题展开讨论。用实验法、问卷调查和社会调查、查资料3种方式进行研究，分析了铅对人体健康的影响及大学生健康与头发中铅含量的关系，提出开发应用天然产物预防铅中毒。结果均与现代医学理论相一致。在教学中开展此项研究，对于复合型人才的培养具有积极的作用。     

图书馆员和谐心理与人文精神关系的初探

论述了图书馆员的和谐心理和人文精神在图书馆和谐服务中的重要作用,分析了馆员不和谐心理产生的主要原因,探讨了实现馆员和谐心理的方法.参6.     

城市室内环境多环芳烃污染与源的相关性

本实验选择了天津市４类典型室内环境和２处室外对照点，共１９个采样点。现场采样测定了１０种ＰＡＨｓ组成含量。结果显示，室内燃煤和室内吸烟是室内环境中多环芳烃排放的主要污染源。同作为对照的室外大气中多环芳烃组成和含量进行了对比，研究了室内环境不同污染源排放多环芳烃组成和含量的特征性。提出了室内燃煤污染同燃煤型室外大气源排放多环芳烃具有相似组成含量特征，而室内烟草烟雾污染源的多环芳烃组成含量特征则与室外     

广州郊区农业土壤重金属含量与土壤性质的关系

研究了广州郊区农业土壤性质和土壤中的重金属含量,并对2者的相关关系进行了分析。结果表明:广州郊区农业土壤中壤土占样品总数的70.5%,土壤pH值平均为5.93,土壤有机质平均含量为31 7g·kg-1,土壤阳离子交换量不高,且各区之间变幅较大;Cu、Pb、Zn、Ni、Cd、As和Hg有效态含量在3种土壤中顺序为菜园土>水稻土>赤红壤;土壤重金属和土壤理化性质间的关系复杂,如有效态Cu、Zn、Ni与土壤有机质含量呈极显著相关,Cd与土壤有机质含量和土壤粘粒含量呈极显著相关,As除与有机质含量和粘粒含量有关,与pH、阳离子交换量也有极显著相关关系。     

The Relationship between the Income Elasticities of Demand and Willingness to Pay

The relationship between income and willingness to pay for collectively provided public/environmental goods is investigated. We show that while the income elasticity of willingness to pay and the ordinary income elasticity of demand are related, knowledge of one is insufficient to determine the magnitude or even the sign of the other. The income elasticity of willingness to pay is influenced by additional factors which are generally unobservable. Examples are provided to illustrate the degree to which the two income elasticities may diverge. Our results indicate that even when goods are demand luxuries they may or may not have income elasticities of willingness to pay which are greater than one.     

上海地区果树根癌病发生与土壤环境的关系

果树根癌病(致病菌为根癌土壤杆菌Agrobacterium tumefaciens)目前在上海地区大面积发生,严重危害桃(Amygdalus persicaL.)、梨(Pyrus bretschneideri Rehd.)、苹果(Malus pumila Mill.)等果树的生长。本文采用化学分析及微生物培养等方法,研究了上海地区桃树和梨树根癌病的发生与土壤因子(土壤含水量、pH值、有机质含量),土壤细菌以及与不同果树品种之间的关系。结果表明:土壤含水量、pH值、有机质含量、土壤中微生物的多样性和微生物总体数目与病害的发生没有显著的相关性,但是土壤中存在的个别菌种对病害的发生有显著的影响,初步分析显示Agrobacterium sp.和Bacillus sp.对根癌病的发生具有抑制作用,而Psuedomonas sp.对根癌病的发生可能具有一定的促进作用。同时不同果树品种的病害发生率之间有显著性的差异。     

Phylogeography of two Atlantic squirrelfishes (Family Holocentridae): exploring links between pelagic larval duration and population connectivity

Genetic surveys of reef fishes have revealed high population connectivity within ocean basins, consistent with the assumption that pelagic larvae disperse long distances by oceanic currents. However, several recent studies have demonstrated that larval retention and self-recruitment may be higher than previously expected. To assess connectivity in tropical reef fishes, we contribute range-wide mtDNA surveys of two Atlantic squirrelfishes (family Holocentridae). The blackbar soldierfish, Myripristis jacobus, has a pelagic juvenile phase of about 58 days, compared to about 71 days (~22% longer) in the longjaw squirrelfish, Holocentrus ascensionis. If the pelagic duration is guiding dispersal ability, M. jacobus should have greater population genetic structure than H. ascensionis. In comparisons of mtDNA cytochrome b sequences from 69 M. jacobus (744 bp) and 101 H. ascensionis (769 bp), both species exhibited a large number of closely related haplotypes (h=0.781 and 0.974, π=0.003 and 0.006, respectively), indicating late Pleistocene coalescence of mtDNA lineages. Contrary to the prediction based on pelagic duration, M. jacobus has much less population structure (φ_ST=0.008, P=0.228) than H. ascensionis (φ_ST=0.091, P<0.001). Significant population partitions in H. ascensionis were observed between eastern, central and western Atlantic, and between Brazil and the Caribbean in the western Atlantic. These results, in combination with the findings from 13 codistributed species, indicate that pelagic larval duration is a poor predictor of population genetic structure in Atlantic reef fishes. A key to understanding this disparity may be the evolutionary depth among corresponding taxonomic groups of “reef fishes”, which extends back to the mid-Cretaceous and encompasses enormous diversity in ecology and life history. We should not expect a simple relationship between pelagic larval duration and genetic connectivity, among lineages that diverged 50–100 million years ago.     

荔枝、龙眼和香蕉质量与生产环境的关系

对广州市的荔枝、龙眼和香蕉三大类水果产品质量和生产环境进行了质量监测和评价。结果表明,这三类水果的生产环境均遭受一定程度的污染;水果质量广泛受到氟污染,部分受到Pb、Cd污染;荔枝对镉的吸收富集作用较强。     

菜园土壤的理化性质和微生物生态特征与种植年限的关系

为了研究菜园土壤生态系统的演化，在广州白云区采集共计64个不同种植年限的菜园土壤样本。对土壤丰要物理化学性质和土壤微生物生态特征进行分析，结果表明：土壤粘粒和微网粒含量、土壤全磷、全钾的含量随菜园土的种植年限而增加；呼吸商则随年限升高，土壤细菌／真菌、放线菌／真菌亦有升高的趋势，而微生物碳氮比、土壤微生物商、微生物氮／全氮随年限降低；种植10a左右菜园土壤的Shannon多样性指数和AWCD值最低，40a土壤最高，80a以上土壤的微生物多样性指标有所降低，表明种植80a后的老菜园土壤微生物生态系统有退化的迹象。     

Relationship between conservation biology and ecology shown through machine reading of 32,000 articles

Conservation biology was founded on the idea that efforts to save nature depend on a scientific understanding of how it works. It sought to apply ecological principles to conservation problems. We investigated whether the relationship between these fields has changed over time through machine reading the full texts of 32,000 research articles published in 16 ecology and conservation biology journals. We examined changes in research topics in both fields and how the fields have evolved from 2000 to 2014. As conservation biology matured, its focus shifted from ecology to social and political aspects of conservation. The 2 fields diverged and now occupy distinct niches in modern science. We hypothesize this pattern resulted from increasing recognition that social, economic, and political factors are critical for successful conservation and possibly from rising skepticism about the relevance of contemporary ecological theory to practical conservation.