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1.
Effects of contrasting modes of larval development on the genetic structures of populations of three species of prosobranch gastropods 总被引:1,自引:0,他引:1
M. G. Hoskin 《Marine Biology》1997,127(4):647-656
In south-eastern Australia, the prosobranch gastropods Morula marginalba (Blainville), Cominella lineolata (Lamarck) and Bedeva hanleyi (Angas) have similar fine-scale distributions, but appear to possess very different dispersal capabilities due to contrasting
modes of larval development. M.marginalba produce planktonic larvae, whereas C. lineolata and B. hanleyi undergo direct development in benthic egg capsules and emerge as crawling juveniles. To test for possible effects of contrasting
life histories on levels of genetic variation within and among populations, a survey was conducted of allozyme variation at
six polymorphic loci in 8 to 9 local populations of each species. Collections of snails were made between June 1992 and November
1993. Sampling ranges spanned between 162 and 180 km of coast. Regardless of larval type, proportions of single-locus genotypes
in each collection were consistent with the recruitment of offspring which had been generated through random mating. However,
genotypic diversity was lower in those species that undergo direct development. Loci surveyed in C. lineolata and B. hanleyi were polymorphic (i.e. frequency of most common allele <95%) in fewer populations than those examined for M.␣marginalba (P <0.001) and, where polymorphisms occurred, also possessed significantly fewer alleles (P <0.001). Consequently, average levels of expected heterozygosity were greater in populations of M. marginalba than in those of either of the other species (P <0.001). Genetic variation among populations, expressed as the standardised variance in allele frequencies (F
ST
), was inversely related to expected larval dispersal capability. The nine collections of M. marginalba showed little overall differentiation (F
ST
= 0.017; P <0.001), reflecting the ability of planktonic larvae to interconnect local populations, and so limit divergence due to drift
and natural selection. In contrast, there were high levels of allelic heterogeneity among the nine collections of C. lineolata (F
ST
= 0.523; P <0.001) and eight collections of B. hanleyi (F
ST
= 0.140; P <0.001). These data imply that for species which undergo direct development, local populations are effectively closed and
evolve largely independent of one another.
Received: 3 May 1996 / Accepted: 12 July 1996 相似文献
2.
The genetic relationships among South-East Asian populations of the scad mackerels Decapterus macarellus, D. macrosoma and D. russelli (Pisces: Carangidae) were investigated. In 1995 and 1996, 216 fish were sampled in seven localities spanning the seas of
Indonesia and were examined for restriction-site polymorphisms using ten restriction enzymes for the mitochondrial (mt) DNA
control region, amplified by the polymerase chain-reaction. The inferred phylogeny of haplotypes led to the recognition of
three distinct mitochondrial lineages or phylads consistent with the distinctions of current taxonomy. All 15 mtDNA haplotypes
found in D. macarellus and all 9 haplotypes found in D. macrosoma were arranged as star-like clusters, suggesting recent evolutionary history. In contrast, the phylad formed by 6 haplotypes
in D. russelli from the Sulawesi Sea exhibited diffuse topology, suggesting that ancestral lineages of this species have been retained to
the present. Average nucleotide-divergence estimates between haplotypes of different phylads were between 0.042 and 0.135,
suggesting ancient separation, in consistency with published allozyme data. High levels of haplotype diversity, but no geographical
heterogeneity, was detected within D. macarellus from the Molucca Sea and the Banda Sea. Populations of D. macrosoma exhibited both significant differences between adjacent regions (Sunda Strait and Java Sea), and broadscale genetic homogeneity
from the South China Sea to the Sulawesi Sea via the Java Sea and Makassar Strait. The geographic isolation of the D. macrosoma population sampled in the Sunda Strait suggests that this region constitute a sharp transition zone between the Indian Ocean
and the Sunda Shelf. Near-monomorphism of haplotypes and low nucleotide diversity (d
X) were observed in the samples of D. macrosoma from the continental shelf (haplotype-diversity estimates, h, = 0.00 to 0.25 ± 0.08 and d
X = 0.000 to 0.002). This was in contrast to the comparatively high haplotype and nucleotide diversities observed in other
pelagic fish species including D. macarellus (h = 0.82 ± 0.05, d
X = 0.012 to 0.015) and D. russelli (h = 0.63 ± 0.12, d
X = 0.016), and in the oceanic D. macrosoma population sampled in the Sunda Strait (h = 0.67 ± 0.31, d
X = 0.005). We hypothesise that this may be the consequence of recent and perhaps repeated bottleneck events that have affected
the D. macrosoma population sampled on the continental shelf.
Received: 29 September 1997 / Accepted: 3 September 1999 相似文献
3.
Mitochondrial d-loop sequences were analyzed to characterize the phylogeographic and population genetic structure of the
northern clingfish (Gobbiesox maeandricus Girard). Sequence analysis of 378 bp from 111 individuals sampled in 14 localities along the northeast Pacific coast and
within the Strait of Georgia from 1996 to 1999 revealed marked genetic differentiation (Φct=0.247) among regional population groupings. The gene genealogy distinguished two major clades of haplotypes separated by
at least 1.1% sequence divergence. One clade with very low haplotype diversity (h=0.2095, n=18) occurred only within the recently unglaciated Strait of Georgia. The other clade had high haplotype diversity (h=0.8808, n=93) and was found in all populations. High haplotype diversity was found in open coastal populations, both north and south
of the maximum extant of the Wisconsin ice sheet, suggesting that the clingfish range was not pushed to a southern refugium
during the last glacial maximum. A nested clade analysis also did not detect a large northward expansion from a single southern
refugium. The level of sequence divergence and coalescent-based analyses suggest that the observed patterns of polymorphism
are the result of Pleistocene diversification within multiple refugia, followed by population expansion and asymmetrical lineage
introgression.
Received: 5 February 2000 / Accepted: 31 August 2000 相似文献
4.
K. A. Tricklebank 《Marine Biology》2000,136(2):337-348
Parma microlepis (Günther) were collected from Malabar, an urban location close to the centre of Sydney, Australia, and from Jervis Bay, a
reference location 170 km south of the city centre. At each location, fish were collected from two sites separated by 100
to 200 m. The ultrastructure of normal liver tissue is described based on 20 female fish collected from Jervis Bay, where
fish are known to be exposed to low levels of organochlorine contaminants. Alterations in the endoplasmic reticulum, mitochondria,
lysosomes and nuclei of hepatocytes were identified and quantified in the liver tissue of fish from this location and compared
to alterations in 20 female fish collected from Malabar, where fish are exposed to higher concentrations of organochlorine
pesticides such as DDT compounds. There were significant differences in the percentage of hepatocytes with swollen mitochondria
(F = 124.025, df = 2, 2, P = 0.008) and atypical nuclei (F = 22.198, df = 2, 2, P = 0.043) between sites (100 to 200 m apart), but there were no clear differences between the percentage of structural alterations
in the hepatocytes of P. microlepis from Jervis Bay and Malabar. Associations between liver morphology and the organochlorines aldrin, dieldrin, DDE and chlordane
were examined using a Pearson correlation matrix. Significant correlations were detected between the percentage of hepatocytes
with dilated endoplasmic reticulum and the concentrations of the pesticide aldrin (r = 0.600, df = 11, r
crit(α = 0.05) = 0.553). Significant associations were also detected between the percentage of hepatocytes with disorganised endoplasmic
reticulum and the concentrations of dieldrin and DDE residues in fish (r = 0.576, r = 0.567, respectively, df = 13, r
crit (α = 0.05) = 0.514). However, there was little evidence that ultrastructural alterations in fish responded to increasing concentrations
of these pesticides in a consistent dose-response manner.
Received: 20 October 1998 / Accepted: 24 November 1999 相似文献
5.
Genetic differentiation among populations of a broadcast spawning soft coral, Sinularia flexibilis, on the Great Barrier Reef 总被引:2,自引:0,他引:2
The genetic structure of 12 reef populations of the soft coral Sinularia flexibilis (Octocorallia, Alcyoniidae) was studied along the Great Barrier Reef (GBR) at a maximum separation of 1,300 km to investigate
the relative importance of sexual and asexual reproduction, genetic differentiation and gene flow among these populations.
S. flexibilis is a widely distributed Indo-Pacific species and a gamete broadcaster that can form large aggregations of colonies on near-shore
reefs of the GBR. Up to 60 individuals per reef were collected at a minimum sampling scale of 5 m at two sites per reef, from
December 1998 to February 2000. Electrophoretic analyses of nine polymorphic allozymes indicated that genotypic frequencies
in most populations and loci did not differ significantly from those expected from Hardy–Weinberg predictions. Analysis of
multi-locus genotypes indicated a high number of unique genotypes (N
go) relative to the number of individuals sampled (N) in each reef population (range of 0.69–0.95). The maximum number of individuals likely to have been produced sexually (N*) was similar to the number of individuals sampled (i.e. N*:N ˜ 1), suggesting that even repeated genotypes may have been produced sexually. These results demonstrated a dominant role
of sexual reproduction in these populations at the scale sampled. Significant genetic differentiation between some populations
indicated that gene flow is restricted between some reefs (F
ST=0.026, 95% CI= 0.011 − 0.045) and even between sites within reefs (F
ST=0.041, 95% CI=0.027 − 0.055). Nevertheless, there was no relationship between geographic separation and genetic differentiation.
Analyses comparing groups of populations showed no significant differentiation on a north-south gradient in the GBR. The pattern
in the number of significant differences in gene frequencies in pairwise population comparisons, however, suggested that gene
flow may be more restricted among inner-shelf reef populations near to the coast than among mid/outer-shelf populations further
from the coast.
Received: 10 July 2000 / Accepted: 5 October 2000 相似文献
6.
Population genetic theory predicts that marine animal species with planktonic larvae will have less genetic structure than
those with direct development. We compared the genetic structure of four species of littorinid snails – two with planktonic
egg capsules that hatch as planktonic larvae and two with benthic egg masses that hatch as crawl-away juveniles. We used DNA
sequencing and single stranded conformational polymorphism (SSCP) to assess sequence variation in a 480 bp fragment of the
mitochondrial cytochrome b gene and then used an analysis of molecular variance (AMOVA) to estimate Φst for populations from the northeastern Pacific coast. One of the two direct-developing species, Littorina subrotundata, had a moderate amount of population structure (Φst=0.209) as expected but the other direct-developing species, L. sitkana, was nearly fixed for a single haplotype that made it impossible to precisely estimate Φst. One of the two planktonic-developing species, L. scutulata, did not show any significant population structure (Φst=0.004). In contrast to our expectations, the other planktonic-developing species, L. plena, showed some weak but statistically significant population structure (Φst=0.052). We discuss how differences in population genetic structure between species with the same type of development may
reflect differences in their historical demography.
Received: 22 December 1999 / Accepted: 24 July 2000 相似文献
7.
Results of isozyme electrophoresis were used to explore the genetic relationships between several Mediterranean morphs of
Cerithium (Gastropoda: Prosobranchia), for which taxonomy is currently uncertain because of high intraspecific variability and low
interspecific differentiation. The large species, classically known as C. vulgatum Bruguière, 1789 was identified at four sites (two in the French Mediterranean and two in southern Spain). Two different larval
types were found in the French sites, but poecilogony could not be demonstrated. Individuals collected from harbours were
not genetically distinct from open-sea populations of classic C. vulgatum. However, a population in the Embiez lagoon (French Mediterranean) which morphologically resembles C. vulgatum did display distinct genetic traits, supporting its status as a separate species. Of the small Cerithium species usually known as C. rupestre, two sympatric species (C.“rupestre” Risso, 1826 and C. lividulum Risso, 1826) were distinguished. Genotype frequencies within the analysed populations revealed much heterozygote deficiency.
F
ST
values (fixation index measuring the effects of population subdivision) suggest a higher genetic differentiation for C. lividulum populations than for C. vulgatum populations. We assume that a high larval dispersal capability (via planktotrophy) allows a high gene flow between populations
of C. vulgatum.
Received: 24 November 1998 / Accepted: 24 September 1999 相似文献
8.
Samples of the Antarctic octopus Pareledone turqueti were taken from three locations on the Scotia Ridge in the Southern Ocean. The genetic homogeneity of these populations was
investigated using isozyme electrophoresis. Whilst panmixia appeared to be maintained around South Georgia (F
ST = 0) gene flow between this island and Shag Rocks, an island only 150 km away but separated by great depths, was extremely
limited (F
ST = 0.74). These results are examined with respect to the discontinuous distribution of P. turqueti throughout Antarctica. An estimate of effective population size was also calculated (N
e = 3600).
Received: 7 March 1997 / Accepted: 27 March 1997 相似文献
9.
The anemone Amphianthus inornata is found at bathyal depths living on colonies of the gorgonian Acanella arbuscula. Previous studies of the morphology and reproductive stage of this anemone, during different times of the year, have indicated
that it reproduces sexually on a seasonal basis. A small proportion of the study population were also reported to be undergoing
asexual reproduction by fission. The anemone Kadosactis commensalis is also bathyal, but lives mainly on the holothurian Paroriza prouhoi. Previous morphological studies have indicated that K. commensalis is a protandrous hermaphrodite that exhibits non-seasonal sexual reproduction only. In the present study, allozyme electrophoresis
was used to examine the prevalence and genetic consequences of asexual reproduction in a population of Amphianthus inornata from 2 200 m in the Rockall Trough, North Atlantic Ocean. Genetic evidence, from five randomly selected polymorphic enzyme
loci, for asexual reproduction in this species was weak. Exact tests indicated that genotype frequencies did not differ significantly
from those expected under Hardy–Weinberg equilibrium. F
IS
(correlation of homologous alleles with reference to local population, assuming random mating) values also did not differ
significantly from zero, and observed heterozygosity (H
o
=0.446) and genotypic diversity (G
o
=17.0387) were very similar to Hardy–Weinberg expected frequencies (H
e
=0.446; G
*
e
=17.0010). Evidence suggests that the contribution of asexual reproduction to recruitment in the study population of A. inornata is low. For a single population of K. commensalis from 4 850 m on the Porcupine Abyssal Plain, North Atlantic Ocean, the hypothesis that inbreeding due to reduced occurrence
of outcrossing between anemones on a single holothurian host was examined by electrophoresis of ten randomly selected enzyme
loci. Single-locus genotypic frequencies were significantly different from expected frequencies for one locus P≤ 0.05, hexokinase-1 (Hex-1)]. F
IS
values were significantly different from zero for two enzyme loci (Hex-1 and Hex-2, P≤ 0.01 and P≤ 0.05, respectively), and the overall observed heterozygosity was lower than the expected heterozygosity (H
o
=0.125, H
e
=0.140). The hypothesis of inbreeding could not be rejected by the present study, although sample size was small (N=55), leading to possible bias in tests of significance. Genetic variation in A. inornata was higher than that recorded for most eukaryotes, although interlocus sampling error for only five loci is high. High genetic
variability has been found in other sea anemones, and has been related to high longevity and mixed reproductive modes. Genetic
variation in K. commensalis was in the higher range of that found in other eukaryotes, and is not unusual for anemones.
Received: 5 August 1996 / Accepted: 11 December 1996 相似文献
10.
We surveyed patterns of allelic variation within twelve samples of the pipi Donax deltoides Lamarck from beaches separated by up to 1200 km but connected to varying degrees by the East Australian Current. We used
these data to test the prediction that the irregular patterns of water movement would cause genetic differentiation in pipis,
so that there would be more genetic variation within and among the more southern regions than the northern regions. We found
that six loci were at least moderately variable within all samples, and there were no clear geographic patterns in allelic
frequencies. In general, genotype frequencies within samples were consistent with predictions for an outcrossed, sexually
reproducing species, and we detected no evidence of population subdivision. Within samples, with the exception of the peptidase
loci, single-locus genotype frequencies were in close agreement with expectations for Hardy–Weinberg equilibrium. We observed
no significant linkage disequilibrium for any pairwise comparison of loci in any sample. Our hierarchical analysis of genetic
variation revealed little variation among all samples (F
st = 0.009). Loci showed consistently low levels of subdivision (F
st from 0.003 to 0.018). We found almost no variation among the four geographic regions sampled (F
rt = 0.001). All variation was therefore attributable to variation among samples within regions (F
sr = 0.010). These data imply that larvae are moving between regions and that levels of present or recent gene flow are high,
and support the conclusions of other studies which have inferred widespread gene flow for animals dispersing via planktonic,
outcrossed larvae in parts of this region. This implies that the East Australian Current is sufficient to produce strong larval
connections despite its intermittent nature. If existing levels of population subdivision reflect current levels of gene flow,
then these data imply that D. deltoides represents a single fishery on the east coast of Australia.
Received: 16 September 1996 / Accepted: 25 September 1996 相似文献
11.
Samples of the scleractinian coral Pocillopora damicornis were collected from six sites located around four islands in the Ryukyu Archipelago, southern Japan, and subjected to allozyme
electrophoresis. Seven polymorphic loci were examined for their allelic patterns. The ratio of observed to expected genotypic
diversity (0.30 < G
o
:G
e
< 0.64), the ratio of the observed number of genotypes to the number of individuals (0.47 < N
g
:N
i
< 0.75), and deviations from Hardy–Weinberg equilibrium indicated that asexual reproduction plays a major role in the maintenance
of established populations. However, populations were not completely dominated by a single or a few clones, and most clones
were represented by only a few individual samples. The high frequency of typhoons in the region suggests that, in P. damicornis, fragmentation caused through occasional exposure to powerful waves is a major mode of asexual reproduction, but asexual
production of planulae may also be contributing to the maintenance of populations. A significant genetic differentiation (F
ST) was found between the six populations examined (0.027 < F
ST < 0.092, average F
ST = 0.056). The moderate gene flow is discussed according to characteristics of the larval stage of the species, and to circulation
patterns in the region.
Received: 7 August 1998 / Accepted: 18 May 1999 相似文献
12.
This paper reports data on 28 allozyme loci in wild and artificially reared sea bass (Dicentrarchus labrax) samples, originating from either coastal lagoon or marine sites in the Mediterranean Sea. F
ST analysis (θ estimator) indicated strong genetic structuring among populations; around 34% of the overall genetic variation
is due to interpopulation variation. Pairwise θ estimates showed that, on average, the degree of genetic structuring was much
higher between marine populations than between samples from lagoons. Six polymorphic loci showed differences in allele frequencies
between marine and lagoon samples. Multivariate analyses of individual allozymic profiles and of allele frequencies suggested
that different arrays of genotypes prevail in lagoons compared to marine samples, particularly at those loci that, on the
basis of previous acclimation experiments, had been implicated in adaptation to freshwater. On the other hand, variation at
“neutral” allozyme loci reflects to a greater extent the geographic location of populations. Allozyme differentiation was
also studied in a D. labrax population from the Portuguese coast. Average genetic distance between this population and the Mediterranean populations
was quite high (Nei's D = 0.236) and calls into question the taxonomic status of the Portuguese population. Finally, genetic relationships between
D. labrax and D. punctatus were evaluated. Average Nei's D was 0.648, revealing high genetic differentiation between the two species, even for two sympatric populations of these species
in Egypt; thus gene flow was not indicated between species.
Received: 24 October 1996 / Accepted: 27 November 1996 相似文献
13.
On the roofs of subtidal crevices, the giant cuttlefish (Sepia apama) of southern Australia lays clutches of lemon-shaped eggs which hatch after 3 to 5 mo. Diffusion of oxygen through the capsule
and chorion membrane to the perivitelline fluid and embryo was modelled using the equation V˙
O2 = G
O2(P
O2out−P
O2in), where V˙
O2 = rate of oxygen consumption, G
O2 = oxygen conductance of the capsule, and P
O2 values = oxygen partial pressures across the capsule. During development, V˙
O2 rose exponentially as the embryo grew, reaching 5.5 μl h−1 at hatching. Throughout development, the capsule dimensions enlarged by absorption of water into the perivitelline space,
increasing G
O2 by a combination of increasing surface area, and decreasing thickness of the capsule. These processes maintained P
O2in high enough to allow unrestricted V˙
O2 until shortly before hatching. Diffusion limitation of respiration in hatching-stage embryos was demonstrated by (1) increased
embryonic V˙
O2 when P
O2out was experimentally raised, (2) greater V˙
O2 of resting individuals immediately after hatching, and (3) reduced V˙
O2 of hatchlings at experimental P
O2 levels higher than P
O2in before hatching. Thus, low P
O2in may be the stimulus to hatch. Potential problems of diffusive gas-exchange are mitigated by the relatively low incubation
temperature (12 °C), which may be a factor limiting the distribution of the species to cool, southern waters.
Received: 14 August 1999 / Accepted: 24 January 2000 相似文献
14.
In this paper we have two goals. First, we examine the effects of sample size on the statistical power to detect a given amount
of inbreeding in social insect populations. The statistical power to detect a given level of inbreeding is largely a function
of the number of colonies sampled. We explore two sampling schemes, one in which a single individual per colony is sampled
for different sample sizes and a second sampling scheme in which constant sampling effort is maintained (the product of the
number of colonies and the number of workers per colony is constant). We find that adding additional workers to a sample from
a colony makes it easier to detect inbreeding in samples from given number of colonies; however, adding more colonies rather
than more workers per colony always gives greater power to detect inbreeding. Because even relatively large amounts of sib-mating
generate relatively small inbreeding coefficients, detection of even substantial deviations from random mating will require
very large samples. Second, we look at the amount of inbreeding in a large population of the western harvest ant, Pogonomyrmex occidentalis. We find deviations from Hardy-Weinberg equilibrium equivalent to approximately 27% sib-mating in our population ( f = 0.09). Review of past studies on the population structure of other Pogonomyrmex species suggests that inbreeding may be a regular feature of the mating system of these ants. Although P. occidentalisis a swarm-mating species, there are a number of features of its population biology which suggest that the effective population
size may be small. These include topographical variation that potentially breaks the population into demes, variation in the
reproductive output of colonies, and variation in the size of reproductives produced by colonies.
Received: 6 May 1996 / Accepted after revision: 6 October 1996 相似文献
15.
F. Maltagliati A. P. Peru M. Casu F. Rossi C. Lardicci M. Curini-Galletti A. Castelli 《Marine Biology》2000,136(5):871-879
The genetic relationships between morphologically indistinguishable marine and brackish populations of Syllis gracilis Grube, 1840 (Polychaeta: Syllidae) were studied by means of allozyme electrophoresis. Samples of S. gracilis from marine coastal and brackish-water habitats were examined for variation at 13 presumptive loci. In addition, a sample
of the closely related species S. prolifera (Krohn, 1852) was analysed. Five loci were multiallelic in at least one population of S. gracilis and eight loci in S. prolifera. Low to moderate levels of within-population genetic variability were found, with average expected heterozygosity values
ranging from H = 0.068 (±0.043 SE) to 0.187 (±0.069 SE) in the populations of S. gracilis; higher values were found in S. prolifera (H = 0.325 ± 0.076). The presence of various private alleles indicated a marked genetic divergence among populations of S. gracilis, with Nei's genetic distances ranging from D = 0.000 to 0.833 and a highly significant F
ST value. Furthermore, evidence for strong genetic heterogeneity between two sympatric marine populations was found. UPGMA cluster
analysis and multidimensional scaling pointed out a clear genetic divergence between brackish and marine populations. At least
two genetically divergent entities occurred in marine and brackish habitats. This could be due to local adaptation of individuals
coming from marine populations to brackish habitats, but more presumably to the occurrence of a species complex within S. gracilis.
Received: 6 June 1999 / Accepted: 7 February 2000 相似文献
16.
Photosynthetic rates of eight seagrass species from Zanzibar were limited by the inorganic carbon composition of natural
seawater (2.1 mM, mostly in the form of HCO3
−), and they exhibited more than three time higher rates at inorganic carbon saturation (>6 mM). The intertidal species that grew most shallowly, Halophila ovalis, Halodule wrightii and Cymodocea rotundata, showed the highest affinity for inorganic carbon (K
1/2 = ca. 2.5 mM), followed by the subtidal species (K
1/2 > 5 mM). Photosynthesis of H. wrightii, C. rotundata, Cymodocea serrulata and Enhalus acoroides was >50% inhibited by acetazolamide, a membrane-impermeable inhibitor of carbonic anhydrase, indicating that extracellular
HCO3
− dehydration is an important part of their inorganic carbon uptake. Photosynthetic rates of H. wrightii, Thalassia hemprichii, Thalassodendron ciliatum, C. serrulata and E. acoroides were strongly reduced by changing the seawater pH from 8.2 to 8.6 in a closed system. In H. ovalis, C. rotundata and Syringodiumisoetifolium, photosynthesis at pH 8.6 was maintained at a higher level than could be caused by the ca. 30% CO2 concentration which remained in the closed experimental systems at that pH, pointing toward HCO3
− uptake in those species. It is suggested that the ability of H. ovalis and C. rotundata to grow in the high, frequently air-exposed, intertidal zone may be related to a capability to take up HCO3
− directly, since this is a more efficient way of HCO3
− utilisation than extracellular HCO3
− dehydration under such conditions. The inability of all species to attain maximal photosynthetic rates under natural conditions
of inorganic carbon supports the notion that seagrasses may respond favourably to any future increases in marine CO2 levels.
Received: 19 March 1997 / Accepted: 31 March 1997 相似文献
17.
Sexuality and cyst formation of the spring-bloom dinoflagellate Scrippsiella hangoei in the coastal northern Baltic Sea 总被引:3,自引:0,他引:3
The temporal sequence and the magnitude of the sexual reproduction and subsequent cyst deposition of the common spring-bloom
dinoflagellate Scrippsiella hangoei (Schiller) Larsen was studied during spring 1996 on the SW coast of Finland, Baltic Sea. The abundances of the different
size of fractions of S. hangoei (14 to 18 μm, 18 to 22 μm and >22 μm) were monitored in the water column, and the deposition of resting cysts was measured
using moored sediment traps. Cyst sedimentation rates were measured throughout the seasonal cycle in order to estimate cyst
resuspension rates for the quantitative assessment of the fraction of population undergoing encystment. The onset of sexual
reproduction, indicated by a significant increase of the small cells (14 to 18 μm) representing gametes, occurred in a nutrient
replete environment well before the exponential growth phase and peak abundances of vegetative cells. Gamete formation was
followed by high abundances of large cells (>22 μm) representing planozygotes, and subsequent sedimentation of resting cysts.
Approximately 60% of the asexually growing bloom population was estimated to form planozygotes, suggesting that encystment
was an important factor in bloom termination and possibly plays a role in the regulation of the magnitude of the bloom. Finally
encystment accounted for 40% of the entire S. hangoei population, resulting in a considerable loss of the bloom population and an input of the vernal phytoplankton biomass to
the benthos.
Received: 11 December 1998 / Accepted: 8 April 1999 相似文献
18.
Allozyme variation was used to investigate the genetic structure of the coral reef fishes Stegastes nigricans, Epinephelus merra and Acanthurus triostegus around New Caledonia. Each species was sampled from each of three sites in the ≃1000 km circumference of the lagoon of New
Caledonia. Allelic variation was recorded for each species at 14, 13 and 17 loci, respectively, and heterozygosity diversity
(H
s) was 0.082, 0.065 and 0.116, respectively. Analysis of genetic differentiation between sites produced inconsistent results
between species, with spatial heterogeneity in two species (S. nigricans, F
st
= 0.038; A. triostegus, F
st = 0.049) and homogeneity in one species (E. merra, F
st = 0.000). Hydrological and climatic data from the lagoon suggest that the eastern and western sides of the lagoon are isolated,
since they lie in water masses of different origin. This may explain the genetic differentiation and restricted gene flow
found at a local scale for S. nigricans and A. triostegus. Homogeneity in populations of E. merra is discussed in relation to its low genetic diversity and its reproductive behaviour.
Received: 23 April 1997 / Accepted: 25 September 1997 相似文献
19.
The nurse shark, Ginglymostoma cirratum, inhabits shallow, tropical, and subtropical waters in the Atlantic and the eastern Pacific. Unlike many other species of
sharks, nurse sharks are remarkably sedentary. We assayed the mitochondrial control region and eight microsatellite loci from
individuals collected primarily in the western Atlantic to estimate the degree of population subdivision. Two individuals
from the eastern Atlantic and one from the Pacific coast of Panama also were genotyped. Overall, the mtDNA haplotype (h = 48 ± 5%) and nucleotide (π = 0.08 ± 0.06%) diversities were low. The microsatellite data mirror the mitochondrial results
with the average number of alleles ([`(N)]A \bar{N}_{A} = 9) and observed heterozygosity ([`(H)]O \bar{H}_{O} = 0.58) both low. The low levels of diversity seen in both the mtDNA and the microsatellite may be due to historical sea
level fluctuations and concomitant loss of shallow water habitat. Eight of the 10 pair-wise western Atlantic F
ST estimates for mtDNA indicated significant genetic subdivision. Pair-wise F
ST values for the microsatellite loci indicated a similar pattern as the mtDNA. The western Atlantic population of nurse sharks
is genetically subdivided with the strongest separation seen between the offshore islands and mainland Brazil, likely due
to deep water acting as a barrier to dispersal. The eastern and western Atlantic populations were closely related. The eastern
Pacific individual is quite different from Atlantic individuals and may be a cryptic, sister species. 相似文献
20.
Growth and grazing responses of tintinnid ciliates feeding on the toxic dinoflagellate Heterocapsacircularisquama 总被引:1,自引:0,他引:1
T. Kamiyama 《Marine Biology》1997,128(3):509-515
Growth and feeding rates of two tintinnid species, Favellaazorica and Favellataraikaensis, were determined under various concentrations of the dinoflagellate Heterocapsacircularisquama which has been reported as highly toxic to shellfish. Mean growth rates of F. azorica and F. taraikaensis on a diet of H.circularisquama (ca. 102 cells ml−1) were 2.15 and 1.97 doublings d−1, respectively. These values are similar to those on a diet of Heterocapsatriquetra which is suitable food for various zooplankton. However, growth rates of both tintinnid species decrease with increasing
concentrations of >103 cells ml−1 of H. circularisquama. In particular, H. circularisquama under conditions of >103 cells ml−1 caused mortality in F.taraikaensis, probably due to toxins. Clearance and ingestion rates of F. azorica on H. circularisquama were 4.1 to 27.5 μl ind−1 h−1 and 1.5 to 28.7 cells ind−1 h−1, respectively, at concentrations of <104 cells ml−1 and those of F. taraikaensis were 0.9 to 22.1 μl ind−1 h−1 and 0.1 to 13.0 cells ind−1 h−1, respectively, at concentrations of <103 cells ml−1. Both clearance and ingestion rates on H.circularisquama were higher for replicates fed on H.triquetra. Daily grazing impact of the two species of Favella on the initial stage of a bloom of H.circularisquama were estimated to reach 6 to 50% of H. circularisquama at a concentration of 540 cells ml−1, indicating that grazing by tintinnids such as Favella spp. may significantly regulate the initial stages of blooms of H. circularisquama.
Received: 3 January 1997 / Accepted: 27 January 1997 相似文献