共查询到13条相似文献,搜索用时 0 毫秒
1.
A reliable and sensitive competitive real-time fluorescent quantitative immuno-PCR (RTFQ-IPCR) assay using a molecular beacon
was developed for the determination of trace fluoranthene (FL) in the environment. Under optimized assay conditions, FL can be
determined in the concentration range from 1 fg/mL to 100 ng/mL, with y = 0.194x + 7.859, and a correlation coe cient of 0.967 was
identified, with a detection limit of 0.6 fg/mL. Environmental water samples were successfully analyzed, recovery was between 90% and
116%, with intra-day relative standard deviation (RSD) of 6.7%–12.8% and inter-day RSD of 8.4%–15.2%. The results obtained from
RTFQ-IPCR were confirmed by ELISA, showing good accuracy and suitability to analyze FL in field samples. As a highly sensitive
method, the molecular beacon-based RTFQ-IPCR is acceptable and promising for providing reliable test results to make environmental
decisions. 相似文献
2.
Determinationofpolychlorinatedbiphenylcongenersinenvironmentalsamples¥ChuShaogang;YangChun;XuXiaobai(ResearchCenterforEco-Env... 相似文献
3.
为了建立用于水环境中大肠杆菌的酶联免疫快速检测方法,针对水中大肠杆菌属多种血清型的特征,制备了水环境样品中大肠杆菌的多特征抗原,包括全菌体抗原、破碎全菌体抗原、菌体抗原、鞭毛抗原和菌毛抗原;采用这5种大肠杆菌抗原分别免疫新西兰大白兔,获得了5种大肠杆菌多克隆抗体,抗体效价高、纯度好,具有较强且稳定的特异性结合抗原的功能;间接ELISA方法表明,全菌体抗体和破碎全菌体抗体的效价均大于1×105,性能优良.基于上述抗体,建立了水中大肠杆菌间接ELISA检测方法,实际水样的检测结果表明,检测限可达104个/L. 相似文献
4.
5.
Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay
Yang Chuanhe Luo Xueyun Liu Chang Li Wenyan Li Yiepeng Zhao Danyu Ji Rong 《环境科学学报(英文版)》1994,6(1):116-122
DetermnationofochratoxinAingrainbymonoclonalantibody-basedenzyme-linkedimmunosorbentassayYangChuanhe;LuoXueyun;LiuChang;LiWen... 相似文献
6.
单克隆抗体间接竞争ELISA测定水体中氟虫腈 总被引:6,自引:0,他引:6
制备了氟虫腈单克隆抗体 ,在此基础上建立了水体中氟虫腈测定的间接竞争性酶联免疫吸附测定 (ELISA)法 .在优化条件下 ,氟虫腈测定的线性浓度范围为 1 0 -1 μg·L-1 ~ 1 0 3μg·L-1 ,最低检出浓度 (I1 0 )为 0 0 8μg·L-1 ,检测灵敏度 (I50 )为6 6 8μg·L-1 ;常见的几种与氟虫腈结构类似的农药的交叉反应率均低于 1 % ,不干扰氟虫腈的检测 ;2 0 %丙酮、5 %甲醇、2 %二甲基甲酰胺、2 %乙腈和 2 %乙酸乙酯基本不影响免疫测定 .利用建立的间接竞争性ELISA方法检测不同水样中氟虫腈 ,结果表明不同水样均可直接进行测定 ,方法的精密度和准确度均符合残留测定的要求 相似文献
7.
采用微波消解土壤并结合螯合树脂类SPE预处理柱去除消解液中重金属,原子荧光光谱法测定土壤中的汞。本方法前处理操作过程简单,用酸量少,避免了测定元素的挥发损失,汞加标回收率为92.8%-104.5%,方法准确度与精密度均令人满意。 相似文献
8.
Determination of herbicides atrazine and butachlor in soil by high performance liquid chromatography
Xu Weibing 《环境科学学报(英文版)》1996,8(4):504-506
Determinationofherbicidesatrazineandbutachlorinsoilbyhighperformanceliquidchromatography¥XuWeibing(ResearchCenterforEco-Envir... 相似文献
9.
Quantity of trace arsenic in grain and soil was determined by hydride nondispersive atomic fluorescence method.Optimum conditions of measurement were selected in the experiment including the pH of the medium, reductive agent, the concentration and flowing velocity of KBH4, rate of carrier gas and atomized temperature. The reference sample of rice C was determined and the linear relationship of the calibration curve was plotted, indicating that the method was highly precise. In the experiment of recovery rate, the method was quite satisfactory.Based on the determination of hundreds of samples, it is proved that atomic fluorescence method is rapid, sensitive and low in interference. It is very efficient on determination of trace arsenic in soils and grains, especially in grains. 相似文献
10.
采用实时定量RT-PCR(Q-RT-PCR)方法,对暴露60d雌二醇的雄性青鳉鱼肝脏内卵黄蛋白原(VTG)和卵壳前体蛋白(CHG)基因表达进行研究.结果表明,0.1,1.0,10.0,100.0ng/L的雌二醇使肝脏内VTG-I,VTG-II,CHG-L和CHG-H基因表达被显著诱导.其中VTG-I显示出较好的剂量—效应关系,是较为理想的生物标记物基因.在0.1ng/L雌二醇暴露组中,VTG-I和VTG-II的表达量均显著高于对照组(P<0.05),表明本方法具有检测环境中低剂量雌二醇暴露的潜力. 相似文献
11.
Determination of 4-tert-octylphenol in surface water samples of Jinan in China by solid phase extraction coupled with GC-MS 总被引:1,自引:0,他引:1
Octylphenols, considered as xenoestrogens, mainly exist as 4-tert-octylphenol (OP) in aquatic environments. The high stability and accumulation of OP in aquatic systems have caused endocrine disruption. The OP in surface water in Jinan, China was analyzed by gas chromatography-mass spectrometry (GC-MS) coupled with solid phase extraction (SPE). Water samples were extracted by SPE on a cartridge system containing C-18 as sorbent. To increase sensitivity and selectivity, OP was derivatized to 4-tert-octyl-phenoxy silane. With the use of phenanthrene-d10 as internal standard, the detection limit based on signal-to-noise ratio (S/N = 3) was 0.06 ng/mL. The average recovery was from 84.67% to 109.7%. The precision of the method given as the relative standard deviations (RSD) was within the range 6.24%-12.96%. In the target water samples, the concentrations of OP were as follows: 15.88-71.24 ng/L for Jinxiuchuan Reservoir, 3.850-26.68 ng/L for the city moat, 6.930-41.56 ng/L for Daming Lake, 66.03-474.2 ng/L for Xiaoqing River, 14.66-17.72 ng/L for the Yellow River, and 10.60-26.43 ng/L for Queshan Reservoir. The Xiaoqing River was seriously polluted due to the discharge of wastewater from Jinan. Jinxiuchuan Reservoir had a higher concentration of OP compared with the Yellow River and Queshan Reservoir, which is ascribed to the surrounding human activities. These data are reported for the first time, providing strong support for the control of OP pollution in Jinan. 相似文献
12.
悬浮液-流动注射-氢化发生原子荧光法测定沉积物中的Pb 总被引:2,自引:0,他引:2
研究了一种把悬浮液进样与氢化发生相结合原子荧光光谱法测定沉积物中Pb的方法。控制悬浮液的粒径在0.088mm以下,用磁力搅拌器搅拌以保证悬浮液分散均匀稳定,方法中以盐酸作介质,K3Fe(CN)6为氧化剂,采用水溶液标准作标准曲线测定了沉积物中的Pb,该法的线性范围为0~200μg/L,检出限为0.26μg/L,回收率为92%~107%,精密度小于6.5%。将此法用于标准物质的分析,测定值均在标准值范围之内,结果令人满意,具有实际应用价值。 相似文献