建德市国家生态示范区建设成效及其成功经验

建德市生态示范区创建工作是以"2066"工程推进、大力建设绿色食品基地和农村三大面源污染治理为主要特点,取得了生态环境明显好转、经济和环境协调发展的建设成效.其结合当地实际的农村面源治理模式等主要成功经验值得推广.     

四川省农村面源污染状况与治理对策研究

四川省农村面源主要分为农田面源、畜禽养殖粪便污染面源和农村生活污染面源,研究根据排放因子法计算了两种主要考核污染物化学需氧量和氨氮的排放量.根据四川省农村面源污染状况特征提出了面源治理对策措施,其中农田面源治理措施包括:推广测土配方施肥、农田面源生态拦截技术等;畜禽养殖污染治理措施包括:加强小型畜禽养殖污染治理,推广农牧结合、干清粪、发酵床处理工艺等;农村生活污染治理措施包括:推进农村生活污水收集及小型污水处理设施等.     

三台县农业面源污染的生态补偿机制与措施研究

三台县是一个农业大县,随着农业经济的迅速发展,农业面源污染日趋严重。种植业污染、畜禽养殖、农村生活污染成为三台县农业面源污染的主要因素。面源污染具有分散性、随机性和监测成本高的特点。对三台县农业面源污染产生进行了经济学分析,农业面源污染属于外部不经济性,通过建立生态补偿机制,控制农业面源污染。以三台县建立生态县和新农村建设为基础,通过转变农业生产方式,调整产业结构,发展生态农业,推进生态村的建设等生态补偿治理措施,改善三台县的农业生态环境。     

发展循环农业治理农村面源污染

农村面源污染主要是指在农村中各项农业生产活动所生产出的污染物对环境中的污染,这部分环境包括但不限于水体、土壤等。农村面源污染主要是过量使用农业化学物质以及各项农业废弃物未被正确处理所导致的,要解决面源污染问题,必须要从带来污染的源头着手,发展生物物质经济、生态型农业以及节约型农业,以循环农业的方式实现面源污染的治理,以此保护我们赖以生存的环境。     

基于广义收益—成本分析的农村面源污染治理策略

应用污染排放系数法、技术经济评价方法,统筹考虑农村面源污染治理的环境效益与经济收益、技术成本与交易费用,构建面向一般区域的农村面源污染治理策略的分析框架与方法.研究表明,2011年宜兴市农村面源污染排放总氮2880.94t,总磷501.19t,水环境经济损失为3.38亿元.耕地测土配方施肥、规模化养殖沼气工程、分散养殖沼气工程和农村生活污水净化工程的净现值率分别为55.00、2.54、0.52、-0.15.综合考虑技术可行性、交易费用和政府管理支撑能力,近期应优先治理规模化养殖和种植业面源污染,分散养殖和农村生活污水污染可作为远期治理重点.     

玉溪市江川区九溪镇区域环境问题及对策探讨

介绍了九溪镇基本情况,分析了九溪镇点源、面源污染现状,从农业面源污染治理、人居环境整治、水土流失防治、畜禽养殖污染治理、河道综合治理、强化环境管理、健全工作机制等方面提出了治理措施,建议加快产业结构优化调整、强化领导工作、严格环境管理、加强农村环境管理体系建设,强化宣传教育、创建环境优美乡镇和生态镇。     

对开展农村环境联片整治的重要性的初步认识及建议

随着农村经济的不断发展,农村环境污染日趋严重。虽然吉林省在农村环境污染防治和生态保护方面取得了一些成效,但农村环保工作仍然十分薄弱。农村环境连片整治示范项目以饮用水水源保护和人居环境质量改善为主要目标,重点支持农村饮用水水源地保护、生活污水和生活垃圾污染治理、畜禽养殖污染治理与综合利用等项目。基于吉林省农村环境存在的共性问题和污染成因分析,有关人士提出了“切实保护农村饮用水源地,加强村庄生活污染治理力度,畜禽养殖污染治理,工矿企业污染治理,农业面源污染治理”等五方面治理措施,有利于促进全省农村环境的有效改善。     

农业面源污染及防治对策

农村面源污染源分布广泛,没有确切位置,其治理一直是个难题.由于化肥和农药的大量使用,农业面源污染已成为我国农村生态环境恶化的重要原因之一.本文对农业面源污染的特点、危害和现状进行了论述,并提出防治农业面源污染的具体防治对策和建议.     

基于加强农村面源污染综合治理的研究

随着农村土地制度的改革和社会经济发展,农村土地得到加速开发,随之而来的是极度追求经济利益的最大化而造成的土地过度开发,从而引发的面源污染问题。绿色发展的背景下,面源污染治理问题是农村发展工作的重中之重,本文探究面源污染的原因,结合当前农村发展现状,提出有效的防范和治理手段。     

倒水河武汉段水环境现状调查与保护对策

针对倒水河武汉段多次出现水质超标现象开展流域范围内水质状况及污染负荷数据收集和现状调研,分析得出流域范围内存在的主要问题为农业农村面源污染形势严峻、污水治理基础设施建设管理不足、水文水资源影响及上游水质恶化,结合倒水河实际提出系列水环境治理措施:农业农村污染控制、城镇生活污染物防治、实施连通湖泊保护工程、完善流域污染监控及联防联控工作机制。     

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis （DGGE） and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity （both in gene richness and diversity index values） and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor （50 mg/kg） recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos （150 mg/kg） caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos （250 mg/kg） produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes （bands） responded differently to the impact of agrochemicals： four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the influence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affiliated with the alpha- and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.     

Arsenic uptake by arbuscular mycorrhizal maize （Zea mays L.） grown in an arsenic-contaminated soil with added phosphorus

The effects of arbuscular mycorrhizal (AM) fungus (Glomus mosseae) and phosphorus (P) addition (100 mg/kg soil) on arsenic (As) uptake by maize plants (Zea mays L.) from an As-contaminated soil were examined in a glasshouse experiment.Non-mycorrhizal and zero-P addition controls were included.Plant biomass and concentrations and uptake of As,P,and other nutrients,AM colonization,root lengths,and hyphal length densities were determined.The results indicated that addition of P significantly inhibited root colonization and development of extraradical mycelium.Root length and dry weight both increased markedly with mycorrhizal colonization under the zero-P treatments,but shoot and root biomass of AM plants was depressed by P application.AM fungal inoculation decreased shoot As concentrations when no P was added,and shoot and root As concentrations of AM plants increased 2.6 and 1.4 times with P addition,respectively.Shoot and root uptake of P,Mn,Cu,and Zn increased,but shoot Fe uptake decreased by 44.6%,with inoculation, when P was added.P addition reduced shoot P,Fe,Mn,Cu,and Zn uptake of AM plants,but increased root Fe and Mn uptake of the nonmycorrhizal ones.AM colonization therefore appeared to enhance plant tolerance to As in low P soil,and have some potential for the phytostabilization of As-contaminated soil,however,P application may introduce additional environmental risk by increasing soil As mobility.     

Degradation of metabolites accumulated of benzo[a]pyrene by coupling Penicillium Chrysogenum with KMnO4

Several main metabolites of benzo[a]pyrene （BaP） formed by Penicillium chrysogenum, Benzo[a]pyrene-1,6-quinone （BP 1,6- quinone）, trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene （BP 7,8-diol）, 3-hydroxybenzo[a]pyrene （3-OHBP）, were identified by high-performance liquid chromatography （HPLC）. The three metabolites were liable to be accumulated and were hardly further metabolized because of their toxicity to microorganisms. However, their further degradation was essential for the complete degradation of BaP. To enhance their degradation, two methods, degradation by coupling Penicillium chrysogenum with KMnO4 and degradation only by Penicillium chrysogenum, were compared; Meanwhile, the parameters of degradation in the superior method were optimized. The results showed that （1） the method of coupling Penicillium chrysogenum with KMnO4 was better and was the first method to be used in the degradation of BaP and its metabolites; （2） the metabolite, BP 1,6-quinone was the most liable to be accumulated in pure cultures; （3） the effect of degradation was the best when the concentration of KMnO4 in the cultures was 0.01% （w/v）, concentration of the three compounds was 5 mg/L and pH was 6.2. Based on the experimental results, a novel concept with regard to the bioremediation of BaP-contaminated environment was discussed, considering the influence on environmental toxicity of the accumulated metabolites.     

Impact of atrazine and nitrogen fertilizers on the sorption of chlorotoluron in soil and model sorbents

Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior of chlorotoluron. At the same concentration of N, sorption of chlorotoluron decreased as the concentration of atrazine increased on the day 0 and 6 in soil, respectively. The sorption of chlorotoluron increased from 0 to 6 d when soils were preincubated with deionized water, ammonium sulfate and urea solution for 6 d. That indicated incubation time was one of the most important factors for the sorption of chlorotoluron in nitrogen fertilizers treatments. The individual sorption isotherms of chlorotoluron in rubbery polymer and silica were strictly linear in single solute system, but there were competition sorption between pesticides or between pesticides and nitrogen fertilizers. That indicated the sorption taken place by concurrent solid-phase dissolution mechanism and sorption on the interface of water-organic matter or water-mineral matter.     

Potential of plant polyphenol oxidases in the decolorization and removal of textile and non-textile dyes

In this study an effort has been made to use plant polyphenol oxidases; potato （Solanum tuberosum） and brinjal （Solanum melongena）, for the treatment of various important dyes used in textile and other industries. The ammonium sulphate fractionated enzyme preparations were used to treat a number of dyes under various experimental conditions. Majority of the treated dyes were maximally decolorized at pH 3.0. Some of the dyes were quickly decolorized whereas others were marginally decolorized. The initial first hour was sufficient for the maximum decolorization of dyes. The rate of decolorization was quite slow on long treatment of dyes. Enhancement in the dye decolorization was noticed on increasing the concentration of enzymes. The complex mixtures of dyes were treated with both preparations of polyphenol oxidases in the buffers of varying pH values. Potato polyphenol oxidase was significantly more effective in decolorizing the dyes to higher extent as compared to the enzyme obtained from brinjal polyphenol oxidase. Decolorization of dyes and their mixtures, followed by the formation of an insoluble precipitate, which could be easily removed simply by centrifugation.     

Removal of heavy metals from sewage sludge by low costing chemical method and recycling in agriculture

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Isolation and preliminary characterization of a 3-chlorobenzoate degrading bacteria

A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several （halo）aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no （chloro）catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.     

Single and joint effects of pesticides and mercury on soil urease

The influence of two pesticides including chlorimuron-ethyl and furadan and mercury （Hg） on urease activity in 4 soils （meadow burozem and phaeozem） was investigated. The soils were exposed to various concentrations of the two pesticides and Hg individually and simultaneously. Results showed that there was a close relationship between urease activity and organic matter content in soil. Chlorimuron-ethyl and furadan could both activate urease in the 4 soils. The maximum increment of urease activity by chlorimuronethyl was up to 14%-18%. There was almost an equal increase （up to 13%-21%） in the urease activity by furadan. On the contrary, Hg markedly inhibited soil urease activity. A logarithmic equation was used to describe the relationship （P〈0.05） between the concentration of Hg and the activity of soil urease in the 4 tested soils. Semi-effect dose （ED50） values by the stress of Hg based on the inhibition of soil urease in the 4 soils were 88, 5.5, 24 and 20 mg/kg, respectively, according to the calculation of the corresponding equations. The interactive effect of chlorimuron-ethyl or furadan with metal Hg on soil urease was mainly synergic at the highest tested concentrations.     

Organochlorine pesticides in soils under different land usage in the Taihu Lake region, China

A field study was conducted in the Taihu Lake region, China in 2004 to reveal the organochlorine pesticide concentrations in soils after the ban of these substances in the year 1983. Thirteen organochlorine pesticides (OCPs) were analyzed in soils from paddy field, tree land and fallow land. Total organochlorine pesticide residues were higher in agricultural soils than in uncultivated fallow land soils. Among all the pesticides, ΣDDX (DDD, DDE and DDT) had the highest concentration for all the soil samples, ranging from 3.10 ng/g to 166.55 ng/g with a mean value of 57.04 ng/g and followed by ΣHCH, ranging from 0.73 ng/g to 60.97 ng/g with a mean value of 24.06 ng/g. Dieldrin, endrin, HCB and α-endosulfan were also found in soils with less than 15 ng/g. Ratios of p,p'-(DDD DDE)/DDT in soils under three land usages were: paddy field ＞ tree land ＞ fallow land, indicating that land usage inlfuenced the degradation of DDT in soils. Ratios of p,p'-(DDD DDE)/DDT ＞1, showing aged residues of DDTs in soils of the Taihu Lake region. The results were discussed with data from a former study that showed very low actual concentrations of HCH and DDT in soils in the Taihu Lake region, but according to the chemical half-lives and their concentrations in soils in 1980s, the concentration of DDT in soils seemed to be underestimated. In any case our data show that the ban on the use of HCH and DDT resulted in a tremendous reduction of these pesticide residues in soils, but there are still high amounts of pesticide residues in soils, which need more remediation processes.     

Alterations of organ histopathology and metallolhionein mRNA expression in silver barb, Puntius gonionotus during subchronic cadmium exposure

Common silver barb,Puntius gonionotus,exposed to the nominal concentration of 0.06 mg/L Cd for 60 d,were assessed for histopathological alterations(gills,liver and kidney),metal accumulation,and metallothionein(MT)mRNA expression.Fish exhibited pathological symptoms such as hypertrophy and hyperplasia of primary and secondary gill lamellae,vacuolization in hepatocytes,and prominent tubular and glomerular damage in the kidney.In addition,kidney accumulated the highest content of cadmium,more than gills and liver.Expression of MT mRNA was increased in both liver and kidney of treated fish.Hepatic MT levels remained high after fish were removed to Cd-free water.In contrast,MT expression in kidney was peaked after 28 d of treatment and drastically dropped when fish were removed to Cd-free water.The high concentrations of Cd in hepatic tissues indicated an accumulation site or permanent damage on this tissue.