EFFECTS OF SELECTED HERBICIDES ON BACTERIAL POPULATIONS IN NONTREATED AND TREATED WATER1

ABSTRACT: Bacterial populations in nontreated and herbicide-treated waters were subjected to three different herbicides. Diuron, dichlobenil, and diquat were added (100 mg/l) to water samples from two fresh water lakes and two herbicide-treated ponds. Total numbers of bacteria were monitored. Bacterial populations in fresh lake water decreased after herbicide additions; however, final populations were significantly greater than the controls. Similar observations were recorded for bacteria in dichlobenil- and diuron-treated waters. Selective enrichment is probably expressed in these experiments.     

BACTERIAL POPULATIONS OF AQUATIC SYSTEMS RECEIVING DIFFERENT TYPES OF STRESS1

ABSTRACT: Bacterial populations in a fast flowing stream receiving chemical wastes and in a slower flowing pond receiving a thermal addition 3–5°C above ambient temperature were compared with populations in a nonpolluted converging stream and an adjacent unheated pond. Parameters measured were total aerobic heterotrophic bacteria, bacterial diversity, percent chromagens of the total bacterial populations, and water temperature. Analyses of monthly samples plated on dilute Standard Plate Count Agar indicated that chemical additives to the waste stream significantly decreased the percent of chromagenic bacteria, and that thermal stress significantly altered total counts and bacterial diversity in the flowing pond. The effects of potential pollutants on these aquatic systems were assessed and are discussed on the basis of results obtained over a 16-month period.     

THE EFFECTS OF DIURON ON BACTERIAL POPULATIONS IN AQUATIC ENVIRONMENTS1

ABSTRACT: The naturally occurring, aerobic, heterotrophic bacterial populations of two impounded ecosystems were studied in a laboratory tank system. One reservoir received runoff from a rural drainage basin only, while the second received treated municipal sewage, industrial waste and heavy recreational use. Water from each reservoir was treated with 1.4 ppm final concentration of diuron and studied for total bacterial counts and nutritional source types. Control tanks were studied concurrently. Total counts increased shortly after diuron addition; however, this was followed by a decrease to a level below the control. Reduction in diversity was significantly greater in water from the polluted reservoir, but chromagenic bacteria were significantly reduced in the non-polluted reservoir. The effects on isolation of specific nutrient types from the two systems following herbicide addition revealed that starch utilizers were the most affected group. Bacteria growing on protein or glucose salts were essentially unaffected. (KEY TERMS: Diuron; Bacteria; Herbicides in Aquatic Environments; Chromagenic Bacteria: Natural Aquatic Bacteria.)     

METABOLIC RESPONSES OF AQUATIC BACTERIAL POPULATIONS TO SELECTED INSECTICIDES1

ABSTRACT: Indigenous bacterial populations from a fresh water reservoir were treated by adding five mg/1 Sevin or Malathion to the water at 23, 28, and 33 C. The degradation of Sevin was evidenced by the increased uptake of O₂ as measured by Warburg manometry. The principal degradation product of Sevin, 1-naphthol, appeared to stimulate metabolic activity of these populations to the same extent as the parent compound. The addition of Malathion to this system appeared, on the other hand, to suppress metabolic activity to some degree. Warburg manometry was shown to be a useful, rapid method for detecting effects of insecticides on aquatic microbial populations.     

RESPONSES OF HETEROTHROPHIC BACTERIAL POPULATIONS TO pH CHANGES IN COAL ASH EFFLUENT1

ABSTRACT: Total culturable heterotrophic bacteria in a coal ash basin and drainage system were monitored over a period of two years. In the first year heavy (bottom) ash was sluiced to the basin resulting in a pH of 6.5. During the second year fly ash was precipitated and added to the sluice lowering the basin pH to 4.6. Sulfate concentrations during 1975 ranged from 16–73 ppm (mean 33) and in 1976 from 44–88 ppm (mean 72). Mean annual basin temperatures were 28.8 and 26.0 C, respectively. Approximately 1500 m in the receiving swamp below the basin, mean pH and temperature were 6.8 and 22.2 C for the first year, and 5.4 and 22.1 C for the second. Total culturable bacteria and diversity (colony types) were reduced at all sampling stations by 44 and 30 percent, respectively, whereas the percentage of the population comprised of chromagenic bacteria increased by 51 percent at the lower pH; Data indicated the pH had a greater effect than did water temperature when temperature was within the range of 15–25 C. The predominant genera within the system in the first year were Bacillus, Sarcina, Achromobacter, Flavobacterium, and Pseudomonas. In the second year, at the lower pH, predominant genera were Pseudomonas, Flavobacterium, Chromobacterium, Bacillus, and Brevibacterium.     

EFFECTS OF NITRATE AND PHOSPHATE CONCENTRATION ON NATURAL AQUATIC BACTERIAL POPULATIONS1

ABSTRACT: Natural aquatic bacterial populations in three streams located at the Savannah River Project, Aiken, S.C. have been studied in relation to the effects of ambient temperatures, dissolved River Project, Aiken, oxygen (DO), nitrate and phosphate concentrations. ³ 3 This work was supported in part by Contract #AT (38–1–824) with the U.S. Atomic Energy Commission, and was done in part in connection with AEC Contract #AT (07–2)–1.
Samples collected at monthly intervals for a period of one year from each system, were plated in duplicate at each of two dilutions on 1/4 strength Standard Plate Count Agar (Difco). After incubation at 25±1 °C for four days, total colony forming units, percent chromagens, and number of colony types (diversity) were determined and colonies were picked for identification. Temperatures were generally equal in two of the streams throughout the year, being lowest in Upper Three Runs (U3R) and Tirns Branch (TB), and highest in the Ash Basin System (ABS). DO content did not vary appreciably between the streams. Nitrates and phosphates were lowest in U3R, next lowest in TB, higher in the last station in ABS, and highest in the ash basin per se. Total colony forming units were highest in the ash basin, whereas chromagen percentage and diversity were highest in the last station in ABS. Results of these studies indicate that high nitrates and phosphates, in the absence of high organic carbon concentrations, have little, if any detrimental effect on the stability of natural aquatic bacterial populations.     

THE EFFECTS OF AGE AND WATER FLUCTUATIONS ON FISH POPULATIONS1

ABSTRACT Two lakes having similar soil types were studied to determine the effects of age and water fluctuations on plankton, benthos and fish populations. Bluff Lake is an older man-made lake which is drawn down in the mid-summer. Oktibbeha County Lake is a young lake and the water levels are maintained. Chemistry data from the two lakes indicate that their chemical properties are very similar. Neither lake would be considered very fertile. Net plankton populations in Bluff Lake and Oktibbeha County Lake were comparable when analyzed on a number of organisms per liter basis. Fluctuations of water levels did not seem to have an effect on the net plankton populations. The benthic population in Bluff Lake is slightly higher than that found in Oktibbeha County Lake. This is true for both numbers and weight per square meter. The species composition of benthic organisms in the two lakes were similar. Based on one-acre samples from each lake, Bluff Lake has a more balanced fish population than does Oktibbeha County Lake. Neither, however, seems to support populations of game fish in which a high percentage of these are in the available or harvestable range. Both lakes contain high populations of gizzard shad.     

AN IN SITU COMPARISON OF THE EFFECTIVENESS OF FOUR ALGICIDES1

ABSTRACT: Four algicides (copper citrate, copper ethanolamine, simazine, and diuron) were tested for effectiveness using in situ bio-assays. Bioassays were conducted in clear polyethylene bags and algicide effectiveness measured by chlorophyll a, productivity, and algal counts. The body of water where this testing took place had a high pH (9.2), and high levels of total dissolved solids (610 mg/l) and nutrients. The order of effectiveness found in this study was simazine > diuron > copper ethanolamine > copper citrate.     

AQUATIC TRANSPORT OF HEAVY METALS IN THE URBAN ENVIRONMENT1

ABSTRACT: A study has been conducted for the past two years on a 4.6 mile stretch of the Saddle River near Lodi, New Jersey. The primary objectives of this study were two fold; initially, the amounts of various heavy metals being contributed to the Saddle River by stormwater runoff, rainfall, and individual tributaries, etc., were investigated to better delineate the distribution of various sources of heavy metals to the aquatic environment. Secondly, a series of benthal deposits from the Saddle River were analyzed to determine the fate of these metals once introduced into the receiving stream. A mass balance analysis of heavy metals in the Saddle River was performed to determine the amount of these materials contributed from unrecorded sources. The results of this study seemed to demonstrate the importance of considering the potential scouring of river sediments as a secondary source of metals in determinations of this type. The distribution of metals in precipitation samples collected in this study was found to be similar to that in runoff, with lead and zinc predominating. Relative concentrations of metals in precipitation as compared to those of stormwater were relatively insignificant. Metal concentrations of bottom sediments were found to vary considerably from sample to sample.     

THE EFFECTS OF DIPOTASSIUM ENDOTHALL ON THE ZOOPLANKTON AND WATER QUALITY OF A SMALL POND1

ABSTRACT: Zooplankton samples and dissolved oxygen-temperature readings and water samples were taken from a treatment and control pond on 41 and 42 separate occasions, respectively, both before and after the application of 5.0 mg/l (a.i.) dipotassium endothall to the 0.31 ha treatment pond on May 31, 1973. Seasonal fluctuations in the density of Cladocera and Copepoda coincided quite closely in both ponds and were similar to the fluctuations reported in the literature to be typical of temperate region ponds and lakes. An apparent seasonal shift in generic composition from .Daphnia spp. in May to Ceriodaphnia spp. in June to Chydorus spp. from July/early September occurred in both ponds and is thought to be at least partially due to fish predation. No apparent changes in species composition or generic densities of Cladocerans was noted in the treatment pond that did not also occur in the control pond. There were no noticeable effects of the dipotassium endothall on either the Calanoida or Cyclopoida suborders of Copepoda. A later pulse of Ostracoda in the treatment pond, when compared to the control, may have been due to the dipotassium endothall or to a combination of the effects of the herbicide on the macrophytes and the method of sampling. A decrease in dissolved oxygen below saturation, occurring in the treated pond from 3–21 days after treatment, was attributed to an increase in biological oxygen demand associated with weed-kill. There was no noticeable increase in plant nutrients (N and P) in the treated pond following herbicide application, nor were there any apparent changes in the other chemical parameters studied.     

EFFECTS OF THE URBAN ENVIRONMENT ON HEAVY RAINFALL DISTRIBUTION1

ABSTRACT: A network of 225 recording raingages was operated over an area of 5200 km² in the St. Louis region during 1971-1975, in conjunction with an extensive investigation of urban effects on precipitation. Study of urban-induced effects on the frequency of heavy rainstorms has revealed a pronounced increase in the occurrence of storms producing 25 mm (1 inch) or more of rain. The increase is greatest in an area that is frequently in the path of storms passing across two urban-industrial regions. Analyses of raincells (rain intensity centers) within heavy convective storms shows a pronounced increase in water yield from cells exposed to potential urban effects, compared with those exposed only to the surrounding rural environment. Naturally-occurring heavy cells tend to undergo the greatest enhancement from urban exposure. Other analyses indicate an above-average frequency of excessive rain rates for periods of five minutes to two hours downwind of the urban-industrial complex. It is concluded that urban-induced intensification of short-duration rainstorms is sufficient to merit inclusion in the design and operation of urban-area hydrologic systems that control the flow of surplus storm water.     

EFFECTS OF SIMULATED CLOUD SEEDING ON STREAMFLOW OF SELECTED WATERSHEDS IN PENNSYLVANIA1

A mathematical model was developed to simulate the hydrologic behavior of five small watersheds in central Pennsylvania. Continuous hydrographs for the 6-month period, April to September 1964, were simulated. Synthesized rainfall cycles consisting of increasing rainfall by 10, 20, and 30 percent to simulate the effects of cloud seeding were processed through the watershed model to determine the effects on low flow augmentation. Other rainfall cycles used consisted of increasing every third storm by 30 percent and of developing a rainfall cycle by processing daily radiosonde data through a mathematical cumulus cloud model to obtain a prediction of rainfall following seeding. A comparison of actual and predicted hydrographs indicated that simulated cloud seeding resulted in significant monthly and seasonal water yields. In general, the results of the study appear to indicate that on a theoretical basis cloud seeding would be a feasible method of augmenting low stream-flow during the summer months on watersheds in the northern Appalachian region.     

EFFECTS OF FOREST HERBICIDE APPLICATIONS ON STREAMWATER CHEMISTRY IN SOUTHWESTERN BRITISH COLUMBIA1

ABSTRACT: The herbicide glyphosate was applied to portions of two watersheds in southwestern British Columbia to kill vegetation that was competing with Pseudotsuga menziesii (Douglas-fir) plantations. This application had little significant effect on streamwater chemistry (K⁺, Na²⁺, Mg²⁺, Ca²⁺, Cl^-, NOs₃^-, NH₄⁺, PO4^3-, SO₄⁼, and SiO₂ concentrations, electrical conductivity, and pH) when vegetation cover in a watershed was reduced by 4%, but had significant (P>0.05) effects, which lasted for at least five years, when cover was reduced by 43%. In this case, most parameters increased in value following the application, with K⁺ and Mg²⁺ concentrations and pH values exhibiting the most prolonged increases and NO₃^- concentrations exhibiting the greatest percentage increases. Sulphate and dissolved SiO₂ concentrations decreased following the application. Streamwater chemical fluxes showed similar trends to concentrations except that changes in fluxes were less significant and no decreases were observed. Forest management induced losses of NO₃-N in streamwater during the first five post-treatment years in the study area decreased in the order: herbicide application (approximately 40 kg/ha) < clearcutting and slashburning (approximately 20 kg/ha) < clearcutting (approximately 10 kg/ha). In watersheds similar to those of the study area, herbicide application is likely to have a greater impact on streamwater chemistry, in general, than would clearcutting or clearcutting followed by slashburning.     

RESPONSE OF BACTERIA TO THE PRESENCE OF CARBARYL IN WATER. I. RESPONSE OF BACTERIAL INDICATORS VS. RESPONSE OF PATHOGENIC BACTERIAL SPECIES1

ABSTRACT: Escherichia coli, Streptococcus faecalis, Salmonella typhimurium, and Staphylococcus aureus were tested by plate counts and Warburg respirometry to determine effects of the presence of carbaryl in water. E. coli and S. typhimurium were initially stimulated in the presence of the insecticide whereas S. faecalis and S. aureus were inhibited. Over a period of days, all bacteria died off more rapidly in the presence of carbaryl. The implications of these data in regards to bacteriological testing of water quality are discussed.     

CONCENTRATION AND SOURCES OF FECAL AND ORGANIC POLLUTION IN AN AGRICULTURAL WATERSHED1

ABSTRACT: Fecal contamination and organic pollution of an agricultural drainage in northeast Indiana was high. Bacterial counts (total coliform, TC; fecal coliform, FC; and fecal streptococcus, FS) and biochemical oxygen demand (BOD) were used to assess waste concentrations. Coliform counts indicated that sections of the drainage receiving septic effluent had waste concentrations far in excess of public health standards (mean FC = 550,000/100 ml). Areas of drainage remote from septic tank pollution were found to occasionally meet federal public health standards for whole body contact recreation but generally these areas had twice the allowable limit of 200 FC/100 ml. Bacterial contamination was highest during runoff events when the median values for TC, FC, and FS were 5, 3, and 17 times greater, respectively, than the median values during low stream discharge. Surface flows carried contaminants from unconfined livestock operations and fecally contaminated sediment was transported by high waters. During one runoff event a BOD loading of 36.7 kg/km² was recorded and the peak BOD concentration observed was 16 mg/l. A discharge of liquid manure from a confined livestock operation caused a major fish kill. Pollution from septic tanks and unconfined livestock is greatest at high stream discharge when dilution reduces the impact on aquatic life.     

THE EFFECTS OF RECENT CHANGES IN ATMOSPHERIC CIRCULATION ON THE HYDROLOGY OF THE KANKAKEE RIVER1

ABSTRACT: In North America the four successive winters from 1974-1975 through 1977–1978 were very different from each other in terms of atmospheric circulation and resulting surface weather conditions. The first year of the sequence there was a near normal circulation pattern. The following years were characterized by the gradual amplification of an upper atmosphere ridge over the West Coast coupled with an eastward displacement of a long-wave trough east of the Rocky Mountains. These changes in circulation brought below normal temperatures to the Midwest, below normal precipition and increasing snowfall which reached record levels in February 1978. These atmospheric changes brought about changes in the flow of the Kankakee River-Total runoff in the winter half-year dropped as precipitation and temperatures dropped; there was a marked retarding of winter runoff and the yield of the watershed increased.     

EFFECTS OF MUNICIPAL WASTEWATER DISCHARGES ON AQUATIC COMMUNITIES,BOISE RWER,IDAHO1

ABSTRACT: Aquatic communities in the Boise River were examined from October 1987 to March 1988 to determine whether they were adversely affected by trace elements in effluents from two Boise wastewater treatment facilities. Trace-element concentrations in the Boise River were less than or near analytical-detection levels and were less than chronic toxicity criteria when detectable. Insect communities colonizing artificial substrates upstream and downstream from the wastewater treatment facilities were strongly associated, and coefficients of community loss indicated that effluents had benign enriching effects. The distributions of trace-element-intolerant mayflies indicated that trace-element concentrations in effluents did not adversely affect intolerant organisms in the Boise River. Condition factor of whitefish was significantly increased downstream from the Lander Street wastewater treatment facility and was significantly decreased downstream from the West Boise wastewater treatment facility.     

EFFECTS OF FOREST FERTILIZATION ON STREAM WATER CHEMISTRY IN THE APPALACHIANS1

ABSTRACT: Stream water chemistry was monitored on two watersheds on the Fernow Experimental Forest in north-central West Virginia to determine the effects of forest fertilization on annual nutrient exports. Ammonium nitrate and triple superphosphate were applied simultaneously at rates of 336 kg ha^?1 N and 224 kg ha^?1 P₂O₅, respectively, which are similar to rates used in commercial forest operations. The treatment significantly increased outputs of several ions. Annual outputs of nitrate N increased as much as 18 times over pretreatment levels, and calcium and magnesium increased as much as three times over pretreatment levels the first year after fertilization. Outputs for these nutrients were elevated for all three post-treatment years. Although nitrate N increased significantly, only about 20 percent of the applied fertilizer was accounted for in stream water exports. Outputs of phosphate P declined following fertilization, probably because the watersheds are phosphorus deficient, but by the third year, they slightly exceeded predicted values. Estimated nutrient losses to deep seepage were substantial, especially on the leakier south-facing catchmeat, on which some nutrient losses were equal to or greater than those in stream water. When the nutrient exports associated with both stream discharge and ground water recharge were combined, the percentages of applied N that were lost were similar on the two watersheds, averaging 27.5 percent. Less than 1 percent of the applied P was lost from either watershed in the combination of streamflow and deep seepage.     

THE EFFECTS OF MINE ACID ON THE POND RIVER WATERSHED IN WESTERN KENTUCKY1

ABSTRACT: H₂SO₄ (sulfuric acid) is formed by a chemical process that occurs in unreclaimed coal mines. The highly toxic acid then flows into the lower swamp areas where it causes considerable damage to the ecosystem. The major effect of the acid is the mass destruction of thousands of trees and various other phreatophytic plants. The contamination is so serious that most of the wildlife has migrated out of the affected area of the swamp in order to survive. Certain geological features such as coal bearing monadanocks make the area somewhat sensitive to mining activities and related geologic hazards. New methods of mine acid abatement make the concept of mass reclamation more realistic than at any time in the past. The constant annihilation of swamp life and processes emphasizes the urgent need for reclamation of the swamp.     

FIRST-YEAR EFFECTS OF CLEARCUTTING AN OAK-HICKORY WATERSHED ON WATER YIELD1

ABSTRACT: Two intermittent streams on oak-hickory watersheds in southern Illinois were gaged with a V-notch weir and sampled with an automatic water sampler. Baseline data was collected for a period of three years. Flow volume showed large variations between years and watersheds. Water samples were analyzed for Na, K, Ca, Mg, ortho-P, and NO₃-N. Water quality was consistently high, but there were significant differences between the watersheds during the calibration period. One watershed was clearcut in November 1979. One year of postharvest data has been analyzed. Flow volume increased 95 percent, but there was no evidence of increased sedimentation. There were significant increases in the stream water concentrations of K, Mg, and NO₃-N of 18 percent, 8 percent, and 274 percent, respectively. Nutrient budgets for the site were not adversely affected by the harvest. The clearcutting operation appears to have had a small impact on the watershed due to minimal disturbance during the logging and below normal precipitation the first year following the harvest.